MD04G1207300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017154
Physical Location & Seq
Forward (+)
29256854 .. 29257558
705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1207300.v1.1.491

Sequence Viewer

Length: 705 bp
ATGCAGTTGATCCTCCACTTCCAATCTCGAGCGGTCCTCTCCAAGCAATCCCTTCGCGTCTCCTACCCAATTGTTAAAACCATAACTTCGCTTCCAAGCAATATCTTTGTTCCAAAATCTCCAATTTTTACCATTCCACTCTCCCTCTGTCGTCAAACCCAGACCCAAAATCCAATTCTACGTGCGGGGAAGAACAAACGGCGCAATGGGTCTCACAGATTAACAAGATTGCTGCTCCAATTGATGCCGGCCATCCTATCGAACTTCAAGATAGTGCCTCGGCCGTTGGATCTGGTTGTTGAAGAATTATGTGGCGGAGACGGCACCGGAGGGGGTCTGGGGATCTGGAAGGGGTTCGGAGGCGGCGGCGATGGGTTCGGAAGAAAACGAAAGAGGAAGTCTCTGTTGTTTGTATTATATGGGGTTCTGGTGATTTGTGGGTTGGGATTGTTGTTCTGTGGAGATGTGGGAAGCAATGTGCTTTTCTGCGGATTGGGAATTGGGCTCTCTAGGGTTGCTATGGCTCAGTGGTGGGACAAGATTGGTATTTTTGCCATCTTTTTTGGGGTTGTTTTGGTGGGTCTAGGGTTTCAGAGAGAGGAATTGCACAAATTGGGTTTGAAGATTAGGGCTTTTTGTCCGGGAATGGAGGCTGTTACTTGGAGAACAAGAGGAAGAAGAAGAGGTGGAAGAAGAGCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

25.78

Weight (kDa)

11.31

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27440
fragaria_vesca FvH4_6g05580
malus_domestica MD04G1207300.v1.1 MD12G1221400.v1.1
prunus_persica Prupe.6G328200_v2.0.a1
pyrus_communis pycom04g18340 pycom12g20750
rosa_chinensis RchiOBHm_Chr3g0454361
rosa_laevigata RLG00000025450 RLG00000025457
rosa_roxburghii Rroxscaffold_6G00425170
rosa_rugosa Rorug02G0654500
rosa_wichuraiana Rw3G004510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 323
AccBSI CCGCTC 1 cut(s) 32
AccII CGCG 1 cut(s) 57
AciI CCGC 6 cut(s) 32, 185, 315, 363, 366, 489
AclWI GGATC 3 cut(s) 4, 297, 350
AcoI YGGCCR 2 cut(s) 249, 281
AgsI TTSAA 3 cut(s) 268, 302, 622
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
Alw26I GTCTC 4 cut(s) 64, 216, 312, 405
AlwI GGATC 3 cut(s) 4, 297, 350
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 2 cut(s) 249, 281
ApeKI GCWGC 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 642
AsuHPI GGTGA 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 219
BccI CCATC 3 cut(s) 260, 365, 563
BceAI ACGGC 3 cut(s) 215, 268, 337
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BcnI CCSGG 1 cut(s) 642
BcoDI GTCTC 4 cut(s) 64, 216, 312, 405
BfaI CTAG 2 cut(s) 510, 584
BisI GCNGC 3 cut(s) 233, 364, 367
BlsI GCNGC 3 cut(s) 234, 365, 368
Bme1390I CCNGG 1 cut(s) 642
Bme18I GGWCC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 642
BmsI GCATC 1 cut(s) 234
BplI GAGNNNNNCTC 4 cut(s) 21, 53, 385, 417
BpuMI CCSGG 1 cut(s) 642
BsaAI YACGTR 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 278
BsaWI WCCGGW 1 cut(s) 326
Bse118I RCCGGY 1 cut(s) 247
Bse3DI GCAATG 2 cut(s) 211, 481
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 1 cut(s) 252
BseMI GCAATG 2 cut(s) 211, 481
BseMII CTCAG 1 cut(s) 539
BseX3I CGGCCG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 219
Bsh1236I CGCG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 284
BshFI GGCC 2 cut(s) 251, 283
BshNI GGYRCC 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 3 cut(s) 248, 327, 641
BslFI GGGAC 1 cut(s) 548
BsmAI GTCTC 4 cut(s) 64, 216, 312, 405
BsmBI CGTCTC 2 cut(s) 64, 312
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 2 cut(s) 251, 283
Bso31I GGTCTC 1 cut(s) 216
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 3 cut(s) 9, 289, 342
BspACI CCGC 6 cut(s) 32, 185, 315, 363, 366, 489
BspANI GGCC 2 cut(s) 251, 283
BspCNI CTCAG 1 cut(s) 538
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 3 cut(s) 4, 297, 350
BspQI GCTCTTC 1 cut(s) 688
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 216
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 211, 481
BsrFI RCCGGY 1 cut(s) 247
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 3 cut(s) 9, 289, 342
Bst6I CTCTTC 2 cut(s) 676, 688
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 3 cut(s) 12, 292, 345
BstMAI GTCTC 4 cut(s) 64, 216, 312, 405
BstMBI GATC 3 cut(s) 9, 289, 342
BstMCI CGRYCG 1 cut(s) 284
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 640
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 219
BstX2I RGATCY 2 cut(s) 289, 342
BstYI RGATCY 2 cut(s) 289, 342
BstZI CGGCCG 1 cut(s) 281
BsuRI GGCC 2 cut(s) 251, 283
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 1 cut(s) 249
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 34
CseI GACGC 1 cut(s) 46
CviJI RGCY 6 cut(s) 251, 283, 505, 524, 632, 653
CviKI_1 RGCY 6 cut(s) 251, 283, 505, 524, 632, 653
DdeI CTNAG 1 cut(s) 525
DpnI GATC 3 cut(s) 11, 291, 344
DpnII GATC 3 cut(s) 9, 289, 342
EaeI YGGCCR 2 cut(s) 249, 281
EagI CGGCCG 1 cut(s) 281
Eam1104I CTCTTC 2 cut(s) 676, 688
EarI CTCTTC 2 cut(s) 676, 688
EciI GGCGGA 1 cut(s) 330
EclXI CGGCCG 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 34
Eco52I CGGCCG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 507
Esp3I CGTCTC 2 cut(s) 64, 312
FaiI YATR 5 cut(s) 83, 310, 418, 420, 521
FaqI GGGAC 1 cut(s) 548
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 3 cut(s) 233, 364, 367
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 3 cut(s) 233, 364, 367
FspBI CTAG 2 cut(s) 510, 584
GlaI GCGC 1 cut(s) 203
GluI GCNGC 3 cut(s) 233, 364, 367
HaeIII GGCC 2 cut(s) 251, 283
HapII CCGG 3 cut(s) 248, 327, 641
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 204
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HpaII CCGG 3 cut(s) 248, 327, 641
HphI GGTGA 1 cut(s) 442
Hpy188I TCNGA 4 cut(s) 359, 380, 594, 704
Hpy188III TCNNGA 3 cut(s) 27, 268, 346
HpyAV CCTTC 2 cut(s) 62, 343
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 4, 607
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 1 cut(s) 181
HspAI GCGC 1 cut(s) 202
KroI GCCGGC 1 cut(s) 247
KroNI GCCGGC 1 cut(s) 249
Kzo9I GATC 3 cut(s) 9, 289, 342
LguI GCTCTTC 1 cut(s) 688
LmnI GCTCC 1 cut(s) 240
LpnPI CCDG 8 cut(s) 173, 261, 278, 323, 331, 340, 413, 654
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 510, 584
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 1 cut(s) 655
MalI GATC 3 cut(s) 11, 291, 344
MbiI CCGCTC 1 cut(s) 32
MboI GATC 3 cut(s) 9, 289, 342
MboII GAAGA 9 cut(s) 202, 314, 393, 634, 687, 690, 693, 702, 705
MfeI CAATTG 2 cut(s) 69, 239
MflI RGATCY 2 cut(s) 289, 342
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 8 cut(s) 69, 123, 174, 239, 305, 498, 602, 611
MmeI TCCRAC 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 75, 221
MspI CCGG 3 cut(s) 248, 327, 641
MspR9I CCNGG 1 cut(s) 642
MunI CAATTG 2 cut(s) 69, 239
Mva1269I GAATGC 1 cut(s) 698
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 321
NaeI GCCGGC 1 cut(s) 249
NciI CCSGG 1 cut(s) 642
NdeII GATC 3 cut(s) 9, 289, 342
NgoMIV GCCGGC 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 325
NmeAIII GCCGAG 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 688
PctI GAATGC 1 cut(s) 698
PdiI GCCGGC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 353
PfoI TCCNGGA 1 cut(s) 640
PkrI GCNGC 3 cut(s) 234, 365, 368
Ppu21I YACGTR 1 cut(s) 182
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 289, 342
SapI GCTCTTC 1 cut(s) 688
SaqAI TTAA 2 cut(s) 75, 221
SatI GCNGC 3 cut(s) 233, 364, 367
Sau3AI GATC 3 cut(s) 9, 289, 342
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 642
SduI GDGCHC 1 cut(s) 507
SetI ASST 2 cut(s) 184, 688
SfaNI GCATC 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 8 cut(s) 69, 123, 174, 239, 305, 498, 602, 611
SsiI CCGC 6 cut(s) 32, 185, 315, 363, 366, 489
SspMI CTAG 2 cut(s) 510, 584
StyD4I CCNGG 1 cut(s) 640
TaiI ACGT 1 cut(s) 184
TaqI TCGA 2 cut(s) 28, 260
TasI AATT 8 cut(s) 69, 123, 174, 239, 305, 498, 602, 611
TauI GCSGC 2 cut(s) 366, 369
Tru1I TTAA 2 cut(s) 75, 221
Tru9I TTAA 2 cut(s) 75, 221
TscAI CASTG 1 cut(s) 533
TseI GCWGC 1 cut(s) 232
TspRI CASTG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 2 cut(s) 510, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.