pycom04g18340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0017154
Physical Location & Seq
Forward (+)
20394439 .. 20395143
705 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18340.1

Sequence Viewer

Length: 705 bp
ATGCAGCTGATCCTCCACTTCCAATCTCGAGCGGTCCTCTCCAAGCAATCCCTTTGCTTCTCATACCCAATTGTTAAAAAAAAAACTTCGCTTCCAAGCAATATCTTTGTTCCAAAATCTCCAATTTTTACCATTCCGCTCTCACTCTGTCGTCAAACCCAGACCCAATATCCAATTCTATGTGCGGGGAAGAAGAATCGGCGCAATGGGTCTCAAAGATTAACAAGATTGCTGCTCCAATTGATGCCGGCCATCGTATCAAACTTCAAGATAGTGCCTCAGCCGTTGGATCTGGTTGTTGAAGAATTATACAGCGGAGACCGCACCGGAGGGGGTCTGGGGATCTGGAAGGGGTTCGGAGGCGGCGGCGATGGGTTCGGAAGAAAACGAAAGAGGAAGTCTTTGTTGCTGTTTCTGTTATATGGGGTTCTGGTGATTTGTGGGTTGGGATTGTTGTTCTGTGGAGATGTGGGAAGCAATGTGCTTTTCTGCGGATTGGGATTTGGGCTTTCGGGGGTTGCTATGGTTCAGTGGTGGGACAAGATTGGTATTTTTACCGTCTTTTTAGGGGGTGTTTTGGTGGGTCTAGGGTTTCAGAGAGAGGAATTGCACAAATTGGGTTTGAAGATTAGGGCTTTTTGTCCAGGAATGGAGGCTGTTACTTGGAGAACAAGAAGAAGAAGAGGTGGAAGAAGAGCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

25.92

Weight (kDa)

10.63

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27440
fragaria_vesca FvH4_6g05580
malus_domestica MD04G1207300.v1.1 MD12G1221400.v1.1
prunus_persica Prupe.6G328200_v2.0.a1
pyrus_communis pycom04g18340 pycom12g20750
rosa_chinensis RchiOBHm_Chr3g0454361
rosa_laevigata RLG00000025450 RLG00000025457
rosa_roxburghii Rroxscaffold_6G00425170
rosa_rugosa Rorug02G0654500
rosa_wichuraiana Rw3G004510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 32, 139
AciI CCGC 8 cut(s) 32, 137, 185, 315, 322, 363, 366, 492
AclWI GGATC 3 cut(s) 4, 297, 350
AcoI YGGCCR 1 cut(s) 249
AgsI TTSAA 3 cut(s) 268, 302, 625
AjnI CCWGG 1 cut(s) 643
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 1 cut(s) 7
AluI AGCT 1 cut(s) 7
Alw26I GTCTC 2 cut(s) 216, 312
AlwI GGATC 3 cut(s) 4, 297, 350
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 2 cut(s) 4, 232
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 1 cut(s) 34
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 16, 219
BccI CCATC 2 cut(s) 260, 365
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 216, 312
BfaI CTAG 1 cut(s) 587
BisI GCNGC 4 cut(s) 5, 233, 364, 367
BlsI GCNGC 4 cut(s) 6, 234, 365, 368
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 645
BmsI GCATC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 21, 53
Bpu10I CCTNAGC 1 cut(s) 279
BsaI GGTCTC 2 cut(s) 216, 312
BsaWI WCCGGW 1 cut(s) 326
Bse118I RCCGGY 1 cut(s) 247
Bse3DI GCAATG 2 cut(s) 211, 484
BseBI CCWGG 1 cut(s) 645
BseMI GCAATG 2 cut(s) 211, 484
BseMII CTCAG 1 cut(s) 293
BseXI GCAGC 2 cut(s) 16, 219
BshFI GGCC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 2 cut(s) 248, 327
BslFI GGGAC 1 cut(s) 551
BsmAI GTCTC 2 cut(s) 216, 312
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 1 cut(s) 251
Bso31I GGTCTC 2 cut(s) 216, 312
BsoBI CYCGRG 1 cut(s) 27
Bsp143I GATC 3 cut(s) 9, 289, 342
BspACI CCGC 8 cut(s) 32, 137, 185, 315, 322, 363, 366, 492
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 1 cut(s) 292
BspPI GGATC 3 cut(s) 4, 297, 350
BspQI GCTCTTC 1 cut(s) 688
BspTNI GGTCTC 2 cut(s) 216, 312
BsrBI CCGCTC 2 cut(s) 32, 139
BsrDI GCAATG 2 cut(s) 211, 484
BsrFI RCCGGY 1 cut(s) 247
BssAI RCCGGY 1 cut(s) 247
BssMI GATC 3 cut(s) 9, 289, 342
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 1 cut(s) 559
Bst6I CTCTTC 2 cut(s) 676, 688
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 3 cut(s) 12, 292, 345
BstMAI GTCTC 2 cut(s) 216, 312
BstMBI GATC 3 cut(s) 9, 289, 342
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 643
BstV1I GCAGC 2 cut(s) 16, 219
BstX2I RGATCY 2 cut(s) 289, 342
BstYI RGATCY 2 cut(s) 289, 342
BsuRI GGCC 1 cut(s) 251
BtgZI GCGATG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 1 cut(s) 249
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 34
CviJI RGCY 6 cut(s) 7, 251, 283, 508, 635, 656
CviKI_1 RGCY 6 cut(s) 7, 251, 283, 508, 635, 656
DdeI CTNAG 1 cut(s) 279
DpnI GATC 3 cut(s) 11, 291, 344
DpnII GATC 3 cut(s) 9, 289, 342
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 2 cut(s) 676, 688
EarI CTCTTC 2 cut(s) 676, 688
Eco31I GGTCTC 2 cut(s) 216, 312
Eco47I GGWCC 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 643
FaiI YATR 6 cut(s) 64, 181, 310, 421, 423, 524
FaqI GGGAC 1 cut(s) 551
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 4 cut(s) 5, 233, 364, 367
Fsp4HI GCNGC 4 cut(s) 5, 233, 364, 367
FspBI CTAG 1 cut(s) 587
GlaI GCGC 1 cut(s) 203
GluI GCNGC 4 cut(s) 5, 233, 364, 367
HaeIII GGCC 1 cut(s) 251
HapII CCGG 2 cut(s) 248, 327
HhaI GCGC 1 cut(s) 204
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HinfI GANTC 1 cut(s) 196
HpaII CCGG 2 cut(s) 248, 327
HphI GGTGA 1 cut(s) 445
Hpy188I TCNGA 4 cut(s) 359, 380, 597, 704
Hpy188III TCNNGA 3 cut(s) 27, 268, 346
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4V TGCA 2 cut(s) 4, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 1 cut(s) 279
HspAI GCGC 1 cut(s) 202
KroI GCCGGC 1 cut(s) 247
KroNI GCCGGC 1 cut(s) 249
Kzo9I GATC 3 cut(s) 9, 289, 342
LguI GCTCTTC 1 cut(s) 688
LmnI GCTCC 1 cut(s) 240
LpnPI CCDG 9 cut(s) 173, 261, 278, 323, 331, 340, 416, 630, 657
Lsp1109I GCAGC 2 cut(s) 16, 219
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 587
MaeIII GTNAC 1 cut(s) 658
MalI GATC 3 cut(s) 11, 291, 344
MbiI CCGCTC 2 cut(s) 32, 139
MboI GATC 3 cut(s) 9, 289, 342
MfeI CAATTG 2 cut(s) 69, 239
MflI RGATCY 2 cut(s) 289, 342
MluCI AATT 7 cut(s) 69, 123, 174, 239, 305, 605, 614
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 9 cut(s) 23, 47, 288, 323, 353, 387, 595, 646, 677
MroNI GCCGGC 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 75, 221
MspA1I CMGCKG 2 cut(s) 7, 315
MspI CCGG 2 cut(s) 248, 327
MspR9I CCNGG 1 cut(s) 645
MunI CAATTG 2 cut(s) 69, 239
Mva1269I GAATGC 1 cut(s) 698
MvaI CCWGG 1 cut(s) 645
MwoI GCNNNNNNNGC 1 cut(s) 321
NaeI GCCGGC 1 cut(s) 249
NdeII GATC 3 cut(s) 9, 289, 342
NgoMIV GCCGGC 1 cut(s) 247
PaeR7I CTCGAG 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 688
PctI GAATGC 1 cut(s) 698
PdiI GCCGGC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 196
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 4 cut(s) 6, 234, 365, 368
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspPI GGNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 289, 342
PvuII CAGCTG 1 cut(s) 7
SapI GCTCTTC 1 cut(s) 688
SaqAI TTAA 2 cut(s) 75, 221
SatI GCNGC 4 cut(s) 5, 233, 364, 367
Sau3AI GATC 3 cut(s) 9, 289, 342
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 645
SetI ASST 2 cut(s) 9, 688
SfaNI GCATC 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 7 cut(s) 69, 123, 174, 239, 305, 605, 614
SsiI CCGC 8 cut(s) 32, 137, 185, 315, 322, 363, 366, 492
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 643
TaaI ACNGT 1 cut(s) 559
TaqI TCGA 1 cut(s) 28
TasI AATT 7 cut(s) 69, 123, 174, 239, 305, 605, 614
TauI GCSGC 2 cut(s) 366, 369
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 2 cut(s) 75, 221
Tru9I TTAA 2 cut(s) 75, 221
TscAI CASTG 1 cut(s) 536
TseI GCWGC 2 cut(s) 4, 232
TspRI CASTG 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.