MD12G1221400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017154
Physical Location & Seq
Forward (+)
29848788 .. 29849495
708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1221400.v1.1.491

Sequence Viewer

Length: 708 bp
ATGCAGCAGATCCTTCACTTCCAATTTCAACCGGTCCTCTCCAAGCAATCTCTTTGCTTCTCCTACCCATTTGTTAAAACCAAAACTTCGCTTCCAAGCAATCTCTTTGCTCCAAAATCTCCAATTTTTACCATTCCACTCTCACTCTATCATCAAATCCAGACCCACAATCCAATTCTATGTGCGAGGAAGAACAAACGCCGCAATGGGTCTCAAAGATTAACAAGATTGCTGCTCCAATTGGTGCCGGCCATCGTATCGAACTTCAAGATACTGCCTCAGCCGCTGGATCTGGTTGTTGAAGAATTCTGCAGCGGAGACGGCAGTGGAGGGGGTCTGGGGATCTGGAAGGGGTTCGGAGGCGGCGGCGATGGGTTTGGAAGAAAACGAAAGAGGAAGTCCTTGTTGCTGTTTCTGTTATATGGGGTTCTGGTGATTTGTGCGTTGGGATTGTTGTTTGGAGGAGATGTTGAAAGCAATGTGCTTTTCTACGGATTGGGATTTGGGCTCTCTGGGGTTGCTATGGTTCAGTGGTGGGACAAGATTGGCATTTTTGCAATCTTTTTTGGGGGTGTTTTGGTGGGTCTAGGGTTTCAGAGAGAGGAATTGCAGAAATTGGGTTTGAAGATTAGGGCTTTTTGTCCCGCAATGGAAACTGTTACTTGGAGAACAAGAAGAAGAAGAAGAAGTGGAAGAAGAGCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

26.27

Weight (kDa)

10.61

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27440
fragaria_vesca FvH4_6g05580
malus_domestica MD04G1207300.v1.1 MD12G1221400.v1.1
prunus_persica Prupe.6G328200_v2.0.a1
pyrus_communis pycom04g18340 pycom12g20750
rosa_chinensis RchiOBHm_Chr3g0454361
rosa_laevigata RLG00000025450 RLG00000025457
rosa_roxburghii Rroxscaffold_6G00425170
rosa_rugosa Rorug02G0654500
rosa_wichuraiana Rw3G004510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AciI CCGC 6 cut(s) 202, 284, 315, 363, 366, 645
AclWI GGATC 3 cut(s) 4, 297, 350
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 305
AgeI ACCGGT 1 cut(s) 31
AgsI TTSAA 5 cut(s) 29, 268, 302, 473, 625
Alw26I GTCTC 2 cut(s) 216, 312
AlwI GGATC 3 cut(s) 4, 297, 350
AlwNI CAGNNNCTG 1 cut(s) 286
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 3 cut(s) 4, 232, 312
ApoI RAATTY 1 cut(s) 305
AsiGI ACCGGT 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 445
AvaII GGWCC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 510
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 3 cut(s) 16, 219, 324
BccI CCATC 2 cut(s) 260, 365
BceAI ACGGC 1 cut(s) 337
BcoDI GTCTC 2 cut(s) 216, 312
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 1 cut(s) 310
BisI GCNGC 7 cut(s) 5, 202, 233, 284, 313, 364, 367
BlsI GCNGC 7 cut(s) 6, 203, 234, 285, 314, 365, 368
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bse118I RCCGGY 2 cut(s) 31, 247
Bse3DI GCAATG 3 cut(s) 211, 484, 654
BseMI GCAATG 3 cut(s) 211, 484, 654
BseMII CTCAG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 3 cut(s) 16, 219, 324
BshFI GGCC 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 244
BshTI ACCGGT 1 cut(s) 31
BsiSI CCGG 2 cut(s) 32, 248
BslFI GGGAC 2 cut(s) 551, 627
BsmAI GTCTC 2 cut(s) 216, 312
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 551, 627
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 1 cut(s) 251
Bso31I GGTCTC 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 3 cut(s) 9, 289, 342
BspACI CCGC 6 cut(s) 202, 284, 315, 363, 366, 645
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 246
BspMAI CTGCAG 1 cut(s) 314
BspPI GGATC 3 cut(s) 4, 297, 350
BspQI GCTCTTC 1 cut(s) 691
BspT107I GGYRCC 1 cut(s) 244
BspTNI GGTCTC 1 cut(s) 216
BsrDI GCAATG 3 cut(s) 211, 484, 654
BsrFI RCCGGY 2 cut(s) 31, 247
BssAI RCCGGY 2 cut(s) 31, 247
BssMI GATC 3 cut(s) 9, 289, 342
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 1 cut(s) 691
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 279
BstKTI GATC 3 cut(s) 12, 292, 345
BstMAI GTCTC 2 cut(s) 216, 312
BstMBI GATC 3 cut(s) 9, 289, 342
BstMWI GCNNNNNNNGC 2 cut(s) 283, 321
BstSFI CTRYAG 1 cut(s) 310
BstV1I GCAGC 3 cut(s) 16, 219, 324
BstX2I RGATCY 3 cut(s) 9, 289, 342
BstYI RGATCY 3 cut(s) 9, 289, 342
BsuRI GGCC 1 cut(s) 251
BtgZI GCGATG 1 cut(s) 384
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 2 cut(s) 331, 536
Cac8I GCNNGC 1 cut(s) 249
CaiI CAGNNNCTG 1 cut(s) 286
Cfr10I RCCGGY 2 cut(s) 31, 247
Cfr13I GGNCC 1 cut(s) 34
CspAI ACCGGT 1 cut(s) 31
CviJI RGCY 4 cut(s) 251, 283, 508, 635
CviKI_1 RGCY 4 cut(s) 251, 283, 508, 635
DdeI CTNAG 1 cut(s) 279
DpnI GATC 3 cut(s) 11, 291, 344
DpnII GATC 3 cut(s) 9, 289, 342
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 691
EarI CTCTTC 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 510
Esp3I CGTCTC 1 cut(s) 312
FaiI YATR 4 cut(s) 181, 421, 423, 524
FaqI GGGAC 2 cut(s) 551, 627
FauI CCCGC 1 cut(s) 652
Fnu4HI GCNGC 7 cut(s) 5, 202, 233, 284, 313, 364, 367
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 7 cut(s) 5, 202, 233, 284, 313, 364, 367
FspBI CTAG 1 cut(s) 587
GluI GCNGC 7 cut(s) 5, 202, 233, 284, 313, 364, 367
HaeIII GGCC 1 cut(s) 251
HapII CCGG 2 cut(s) 32, 248
HpaII CCGG 2 cut(s) 32, 248
HphI GGTGA 1 cut(s) 445
Hpy188I TCNGA 3 cut(s) 359, 597, 707
Hpy188III TCNNGA 3 cut(s) 160, 268, 346
HpyAV CCTTC 2 cut(s) 23, 343
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4V TGCA 4 cut(s) 4, 312, 557, 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 321
HpyF3I CTNAG 1 cut(s) 279
KroI GCCGGC 1 cut(s) 247
KroNI GCCGGC 1 cut(s) 249
Kzo9I GATC 3 cut(s) 9, 289, 342
LguI GCTCTTC 1 cut(s) 691
LmnI GCTCC 2 cut(s) 115, 240
LpnPI CCDG 9 cut(s) 45, 173, 261, 272, 278, 323, 331, 416, 498
Lsp1109I GCAGC 3 cut(s) 16, 219, 324
MaeI CTAG 1 cut(s) 587
MaeIII GTNAC 1 cut(s) 658
MalI GATC 3 cut(s) 11, 291, 344
MboI GATC 3 cut(s) 9, 289, 342
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 3 cut(s) 9, 289, 342
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 7 cut(s) 23, 123, 174, 239, 305, 605, 614
MnlI CCTC 8 cut(s) 47, 180, 288, 323, 353, 387, 455, 595
MroNI GCCGGC 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 75, 221
MspA1I CMGCKG 2 cut(s) 286, 315
MspI CCGG 2 cut(s) 32, 248
MunI CAATTG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 701
MwoI GCNNNNNNNGC 2 cut(s) 283, 321
NaeI GCCGGC 1 cut(s) 249
NdeII GATC 3 cut(s) 9, 289, 342
NgoMIV GCCGGC 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 691
PctI GAATGC 1 cut(s) 701
PdiI GCCGGC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 31
PkrI GCNGC 7 cut(s) 6, 203, 234, 285, 314, 365, 368
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 34
PstI CTGCAG 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 286
PsuI RGATCY 3 cut(s) 9, 289, 342
SapI GCTCTTC 1 cut(s) 691
SaqAI TTAA 2 cut(s) 75, 221
SatI GCNGC 7 cut(s) 5, 202, 233, 284, 313, 364, 367
Sau3AI GATC 3 cut(s) 9, 289, 342
Sau96I GGNCC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 510
SfcI CTRYAG 1 cut(s) 310
SinI GGWCC 1 cut(s) 34
Sse9I AATT 7 cut(s) 23, 123, 174, 239, 305, 605, 614
SsiI CCGC 6 cut(s) 202, 284, 315, 363, 366, 645
SspMI CTAG 1 cut(s) 587
TaaI ACNGT 1 cut(s) 658
TaqI TCGA 1 cut(s) 260
TasI AATT 7 cut(s) 23, 123, 174, 239, 305, 605, 614
TauI GCSGC 4 cut(s) 204, 286, 366, 369
Tru1I TTAA 2 cut(s) 75, 221
Tru9I TTAA 2 cut(s) 75, 221
TscAI CASTG 2 cut(s) 331, 536
TseI GCWGC 3 cut(s) 4, 232, 312
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 2 cut(s) 331, 536
VpaK11BI GGWCC 1 cut(s) 34
XapI RAATTY 1 cut(s) 305
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.