MD04G1220700.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016865
Physical Location & Seq
Forward (+)
30186311 .. 30187294
984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1220700.v1.1.491

Sequence Viewer

Length: 984 bp
ATGATCCCAGCATGTTTCAGCCAGCCCAACACACCCACAAGCACCTCTCAAGTGCCTCAAAACCTCATAACCTGCATATACCAAACTCAGCTTTGCAACTCTCCCACATATCTTACTCTCACCTGGTCCAAAACCCTCTTCTCTCACTCCCTCACCATCCATGCCTCTAACTCTTTCTCCCTCACTATCTCTCTCAACCCGTCAACCTTCTCGTTCTTTCGAACCAGACCCGGTTCCAAATCCATCTCTCTAACCCACTGCCACCACAAAAGAATCAAGCTTTACTGGGACTTCACCCGGGCTGACTTCACTCACAACTCCGCCGAGCCCGAGTCTTGCTTCTACATCGCCATTTCTTGCAATGCAAGGCTTGAATTCTTTCTGGGGGATCTTTCGGACGAGTTGACTCAGCGATCAGGGTTACTTCTGACTCACAAGCTCAGCCAACCGGCTTTACTGTCAAGGCGGGAACATGTGTTCGGGCGGAGGAATTACATATCCAGAGCTCAGTTCTTGGGGTCCAAACACGAAATCGGAATAGAGTGCAGCGAGGGAACACTTAGAGTAAAAGTAGATGGAGTAACAAGCCTCGTTGTCAAAAGGTTGGCTTGGAAATTCAGAGGCAATGAGAAGATTTTTGTTGGCGGAGTAGAATTTGAATTTTATTGGGATGTCTTCAATTGGGTTAATAATCATGGTGGCAGTAATGGGAACGGTCATGGTGTATTTGTATTTCAAGTTGGCGATGGTGGAGTGTGGCCGGAAATGGTAGGTCCTGAGAAGAAGTTGATGAGGAAGAGCTTGTCAACGTCGGCTGCTTCAGCATCAATGTCATCGGTGTCTCCATCGCCGTCGTGCTCGAGCGTGCTGCAATGGGCAGATGAAAGTAGTGATGGGGGTAGGAGTTCATGTTCTTCATCAACTAGGTCGTGTGGTAGTAATGGAGGATTTTCTCTGTTGTTGTATGCTTGGAAGAGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

328

Amino Acids

36.27

Weight (kDa)

9.01

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 19 - 325 7.5e-77 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016865)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03450
malus_domestica MD04G1220700.v1.1 MD12G1237000.v1.1
prunus_persica Prupe.6G339700_v2.0.a1
pyrus_communis pycom04g19530
rosa_laevigata RLG00000025635
rosa_multiflora Rmu_sc0006510.1_g000002
rosa_roxburghii Rroxscaffold_164G00436180 Rroxscaffold_6G00429120
rosa_rugosa Rorug02G0637700
rosa_samantha Rh3BG040700 Rh3CG039200 Rh3DG039900
rosa_wichuraiana Rw3G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 80
AciI CCGC 4 cut(s) 321, 466, 484, 645
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 758
AcsI RAATTY 4 cut(s) 374, 614, 653, 659
AcuI CTGAAG 1 cut(s) 804
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 4 cut(s) 374, 659, 679, 737
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AloI GAACNNNNNNTCC 4 cut(s) 461, 493, 895, 927
AluBI AGCT 5 cut(s) 91, 280, 439, 506, 801
AluI AGCT 5 cut(s) 91, 280, 439, 506, 801
Alw21I GWGCWC 2 cut(s) 508, 860
Alw26I GTCTC 1 cut(s) 846
AlwI GGATC 1 cut(s) 396
Ama87I CYCGRG 3 cut(s) 297, 329, 859
AoxI GGCC 1 cut(s) 758
ApeKI GCWGC 3 cut(s) 546, 815, 868
ApoI RAATTY 4 cut(s) 374, 614, 653, 659
Asp700I GAANNNNTTC 1 cut(s) 378
AspS9I GGNCC 3 cut(s) 126, 519, 773
AsuC2I CCSGG 3 cut(s) 231, 298, 299
AsuHPI GGTGA 3 cut(s) 112, 145, 286
AsuII TTCGAA 1 cut(s) 220
AvaI CYCGRG 3 cut(s) 297, 329, 859
AvaII GGWCC 3 cut(s) 126, 519, 773
BanII GRGCYC 2 cut(s) 330, 508
BbsI GAAGAC 1 cut(s) 667
Bbv12I GWGCWC 2 cut(s) 508, 860
BbvI GCAGC 3 cut(s) 558, 802, 855
BccI CCATC 6 cut(s) 164, 251, 569, 740, 853, 887
BceAI ACGGC 1 cut(s) 835
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 3 cut(s) 231, 298, 299
BcoDI GTCTC 1 cut(s) 846
BfaI CTAG 1 cut(s) 924
BfuAI ACCTGC 1 cut(s) 80
BisI GCNGC 3 cut(s) 547, 816, 869
BlpI GCTNAGC 1 cut(s) 440
BlsI GCNGC 3 cut(s) 548, 817, 870
Bme1390I CCNGG 4 cut(s) 124, 231, 298, 299
Bme18I GGWCC 3 cut(s) 126, 519, 773
BmeT110I CYCGRG 3 cut(s) 297, 329, 859
BmgT120I GGNCC 3 cut(s) 126, 519, 773
BmiI GGNNCC 2 cut(s) 235, 520
BmrFI CCNGG 4 cut(s) 124, 231, 298, 299
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 667
Bpu1102I GCTNAGC 1 cut(s) 440
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 33
BpuMI CCSGG 3 cut(s) 231, 298, 299
BsaJI CCNNGG 1 cut(s) 297
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 895, 925
Bse118I RCCGGY 1 cut(s) 448
Bse1I ACTGG 1 cut(s) 290
Bse3DI GCAATG 3 cut(s) 367, 631, 878
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 2 cut(s) 156, 676
BseMI GCAATG 3 cut(s) 367, 631, 878
BseMII CTCAG 5 cut(s) 101, 422, 454, 521, 768
BseNI ACTGG 1 cut(s) 290
BseXI GCAGC 3 cut(s) 558, 802, 855
BseYI CCCAGC 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 565
BshFI GGCC 1 cut(s) 760
BsiHKAI GWGCWC 2 cut(s) 508, 860
BsiHKCI CYCGRG 3 cut(s) 297, 329, 859
BsiSI CCGG 4 cut(s) 231, 298, 449, 761
BslFI GGGAC 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 846
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 1 cut(s) 760
BsoBI CYCGRG 3 cut(s) 297, 329, 859
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 3 cut(s) 330, 508, 860
Bsp143I GATC 3 cut(s) 3, 388, 413
Bsp1720I GCTNAGC 1 cut(s) 440
BspACI CCGC 4 cut(s) 321, 466, 484, 645
BspANI GGCC 1 cut(s) 760
BspCNI CTCAG 5 cut(s) 100, 421, 453, 520, 769
BspLI GGNNCC 2 cut(s) 235, 520
BspMI ACCTGC 1 cut(s) 80
BspPI GGATC 1 cut(s) 396
BspQI GCTCTTC 1 cut(s) 791
BspT104I TTCGAA 1 cut(s) 220
BsrDI GCAATG 3 cut(s) 367, 631, 878
BsrFI RCCGGY 1 cut(s) 448
BsrI ACTGG 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 3 cut(s) 3, 388, 413
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 2 cut(s) 459, 716
Bst6I CTCTTC 3 cut(s) 143, 791, 968
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 2 cut(s) 23, 866
BstDEI CTNAG 6 cut(s) 87, 408, 440, 507, 560, 777
BstF5I GGATG 2 cut(s) 156, 676
BstKTI GATC 3 cut(s) 6, 391, 416
BstMAI GTCTC 1 cut(s) 846
BstMBI GATC 3 cut(s) 3, 388, 413
BstMWI GCNNNNNNNGC 1 cut(s) 821
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 15, 476
BstSCI CCNGG 4 cut(s) 122, 229, 296, 297
BstV1I GCAGC 3 cut(s) 558, 802, 855
BstV2I GAAGAC 1 cut(s) 667
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 1 cut(s) 760
BtgZI GCGATG 3 cut(s) 331, 759, 831
BtsCI GGATG 2 cut(s) 156, 676
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 2 cut(s) 23, 866
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 3 cut(s) 126, 519, 773
Cfr9I CCCGGG 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 122
CviAII CATG 6 cut(s) 12, 161, 473, 695, 719, 909
DdeI CTNAG 6 cut(s) 87, 408, 440, 507, 560, 777
DpnI GATC 3 cut(s) 5, 390, 415
DpnII GATC 3 cut(s) 3, 388, 413
EaeI YGGCCR 1 cut(s) 758
Eam1104I CTCTTC 3 cut(s) 143, 791, 968
EarI CTCTTC 3 cut(s) 143, 791, 968
EciI GGCGGA 3 cut(s) 310, 499, 660
Ecl136II GAGCTC 1 cut(s) 506
Eco24I GRGCYC 2 cut(s) 330, 508
Eco47I GGWCC 3 cut(s) 126, 519, 773
Eco53kI GAGCTC 1 cut(s) 506
Eco57I CTGAAG 1 cut(s) 804
Eco88I CYCGRG 3 cut(s) 297, 329, 859
EcoICRI GAGCTC 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 773
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 122
EcoT38I GRGCYC 2 cut(s) 330, 508
FaeI CATG 6 cut(s) 15, 164, 476, 698, 722, 912
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 302
FatI CATG 6 cut(s) 11, 160, 472, 694, 718, 908
FauI CCCGC 1 cut(s) 459
Fnu4HI GCNGC 3 cut(s) 547, 816, 869
FokI GGATG 2 cut(s) 143, 683
FriOI GRGCYC 2 cut(s) 330, 508
Fsp4HI GCNGC 3 cut(s) 547, 816, 869
FspBI CTAG 1 cut(s) 924
GluI GCNGC 3 cut(s) 547, 816, 869
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 1 cut(s) 760
HapII CCGG 4 cut(s) 231, 298, 449, 761
Hin1II CATG 6 cut(s) 15, 164, 476, 698, 722, 912
HincII GTYRAC 3 cut(s) 204, 405, 807
HindII GTYRAC 3 cut(s) 204, 405, 807
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 4 cut(s) 273, 332, 406, 430
HpaII CCGG 4 cut(s) 231, 298, 449, 761
HphI GGTGA 3 cut(s) 112, 145, 286
Hpy166II GTNNAC 3 cut(s) 204, 405, 807
Hpy188I TCNGA 4 cut(s) 397, 429, 536, 620
Hpy188III TCNNGA 2 cut(s) 501, 776
Hpy8I GTNNAC 3 cut(s) 204, 405, 807
Hpy99I CGWCG 2 cut(s) 814, 856
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 2 cut(s) 459, 716
HpyCH4IV ACGT 1 cut(s) 809
HpyCH4V TGCA 6 cut(s) 75, 96, 360, 365, 546, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 821
HpyF3I CTNAG 6 cut(s) 87, 408, 440, 507, 560, 777
HpySE526I ACGT 1 cut(s) 809
Hsp92II CATG 6 cut(s) 15, 164, 476, 698, 722, 912
Kzo9I GATC 3 cut(s) 3, 388, 413
LguI GCTCTTC 1 cut(s) 791
Lsp1109I GCAGC 3 cut(s) 558, 802, 855
LweI GCATC 1 cut(s) 833
MabI ACCWGGT 1 cut(s) 122
MaeI CTAG 1 cut(s) 924
MaeII ACGT 1 cut(s) 809
MaeIII GTNAC 2 cut(s) 420, 580
MalI GATC 3 cut(s) 5, 390, 415
MboI GATC 3 cut(s) 3, 388, 413
MboII GAAGA 6 cut(s) 130, 643, 667, 793, 808, 906
MfeI CAATTG 1 cut(s) 679
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 3 cut(s) 330, 508, 860
MluCI AATT 6 cut(s) 374, 490, 614, 653, 659, 679
MlyI GAGTC 3 cut(s) 341, 400, 424
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 1 cut(s) 687
MspI CCGG 4 cut(s) 231, 298, 449, 761
MspR9I CCNGG 4 cut(s) 124, 231, 298, 299
MunI CAATTG 1 cut(s) 679
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 821
NciI CCSGG 3 cut(s) 231, 298, 299
NdeII GATC 3 cut(s) 3, 388, 413
NlaIII CATG 6 cut(s) 15, 164, 476, 698, 722, 912
NlaIV GGNNCC 2 cut(s) 235, 520
NmeAIII GCCGAG 1 cut(s) 349
NspI RCATGY 2 cut(s) 15, 476
NspV TTCGAA 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 859
PciI ACATGT 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 273
PkrI GCNGC 3 cut(s) 548, 817, 870
PleI GAGTC 3 cut(s) 340, 400, 424
PpsI GAGTC 3 cut(s) 340, 400, 424
PpuMI RGGWCCY 1 cut(s) 773
PscI ACATGT 1 cut(s) 472
Psp124BI GAGCTC 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 773
Psp6I CCWGG 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 235, 520
PspPI GGNCC 3 cut(s) 126, 519, 773
PspPPI RGGWCCY 1 cut(s) 773
PspXI VCTCGAGB 1 cut(s) 859
PsuI RGATCY 1 cut(s) 388
SacI GAGCTC 1 cut(s) 508
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 3 cut(s) 547, 816, 869
Sau3AI GATC 3 cut(s) 3, 388, 413
Sau96I GGNCC 3 cut(s) 126, 519, 773
SchI GAGTC 3 cut(s) 341, 400, 424
ScrFI CCNGG 4 cut(s) 124, 231, 298, 299
SduI GDGCHC 3 cut(s) 330, 508, 860
SexAI ACCWGGT 1 cut(s) 122
SfaNI GCATC 1 cut(s) 833
Sfr274I CTCGAG 1 cut(s) 859
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 3 cut(s) 126, 519, 773
SlaI CTCGAG 1 cut(s) 859
SmaI CCCGGG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 48, 859
SmoI CTYRAG 2 cut(s) 48, 859
Sse9I AATT 6 cut(s) 374, 490, 614, 653, 659, 679
SsiI CCGC 4 cut(s) 321, 466, 484, 645
SspMI CTAG 1 cut(s) 924
SstI GAGCTC 1 cut(s) 508
StyD4I CCNGG 4 cut(s) 122, 229, 296, 297
TaaI ACNGT 2 cut(s) 459, 716
TaiI ACGT 1 cut(s) 812
TaqI TCGA 2 cut(s) 220, 860
TasI AATT 6 cut(s) 374, 490, 614, 653, 659, 679
TfiI GAWTC 1 cut(s) 273
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 1 cut(s) 263
TseI GCWGC 3 cut(s) 546, 815, 868
TspDTI ATGAA 3 cut(s) 897, 897, 906
TspMI CCCGGG 1 cut(s) 297
TspRI CASTG 1 cut(s) 263
VpaK11BI GGWCC 3 cut(s) 126, 519, 773
XapI RAATTY 4 cut(s) 374, 614, 653, 659
XceI RCATGY 2 cut(s) 15, 476
XhoI CTCGAG 1 cut(s) 859
XmaI CCCGGG 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.