MD12G1237000.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016865
Physical Location & Seq
Forward (+)
31082695 .. 31083687
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1237000.v1.1.491

Sequence Viewer

Length: 993 bp
ATGATCCCAGCATGTTTCGGCCAGCCCAACACACCCACAAGCAGCTCTCAAGTGCCCCAAAACCTCATAACCTGCATATACCAAACTCAGCTTTGCAACTCTCCCACATATCTCACTCTCACCTGGTCCAAAACCTTCTTCTCTCACTCCCTCACCATCCATGCCTCTGACTCGTTCTCCTTCACCGTCTCCTTCAACCCATCAACCTTCTCGTTCTTTCGAACCAGACCGGGCTCCAAATCCATCTCTCTAACCCACAGCAAACACAAAAGCATCAAGCTCTACTGGGACTTCACCCGGGCTGACTTCACTCACAACTCCGCCGAGGCCGAGTCATGCTTCTACATCGCCATTTCTTGCAATGCAAGGCTTGAATTCTTTCTTGGGGATCTTCTGGACGATTTGATGGGGCGATCAGGGTTAGTTCCGACTCACAAGCTCGGCAAACCAGCACTACTGTCAAGGCGGGAACATGTGTTTGGACACAGGAATTACATATCCAGAGCTCAGTTCTTGGGATCCAAACACGAAATTGGAATAGAGTGTAGTAAAGGTACACTTCGACTAAAAGTAGACGGAATAACAAGCCTAGTTGTCAAAAGGTTGGCTTGGAAATTCAGAGGCAATGAGAAGATTTTTGTTGGCGGAGTAGAATTTGAATTTTATTGGGATGTCTTCAATTGGGTTAATAATGAAGGTGGTAGTAATGGGAACGGTCATGGTGTGTTTGTATTTCAAGTCGGCGATGGTGGAGTGTGGCCGGAAATGGTAGGTCCTGAAAAGAAGTTGATGAGGAAGAGCTTGTCAATGTCGACTGCTTCGACAACAGTGCCGTCAATTGCCTCGCTGTCTCCATCACCATCGTGCTCGAGCGTGCTGCAATGGGCAGAGGAAAGTAGTGATGGGGGGAGGAGTTCATGTTCTTCATCAACTAGGTCATGTGGTAGTAATGGGGGGTTTTCTCTGTTGTTGTATGCTTGGAAGAGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

331

Amino Acids

36.49

Weight (kDa)

9.03

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 19 - 328 1e-72 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016865)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03450
malus_domestica MD04G1220700.v1.1 MD12G1237000.v1.1
prunus_persica Prupe.6G339700_v2.0.a1
pyrus_communis pycom04g19530
rosa_laevigata RLG00000025635
rosa_multiflora Rmu_sc0006510.1_g000002
rosa_roxburghii Rroxscaffold_164G00436180 Rroxscaffold_6G00429120
rosa_rugosa Rorug02G0637700
rosa_samantha Rh3BG040700 Rh3CG039200 Rh3DG039900
rosa_wichuraiana Rw3G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 80
AccI GTMKAC 2 cut(s) 573, 812
AciI CCGC 3 cut(s) 321, 466, 645
AclWI GGATC 3 cut(s) 396, 513, 526
AcoI YGGCCR 2 cut(s) 19, 758
AcsI RAATTY 4 cut(s) 374, 614, 653, 659
AfaI GTAC 1 cut(s) 556
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 5 cut(s) 196, 374, 659, 679, 737
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 6 cut(s) 122, 154, 366, 398, 462, 494
AleI CACNNNNGTG 1 cut(s) 862
AloI GAACNNNNNNTCC 2 cut(s) 904, 936
AluBI AGCT 6 cut(s) 45, 91, 280, 439, 506, 801
AluI AGCT 6 cut(s) 45, 91, 280, 439, 506, 801
Alw21I GWGCWC 2 cut(s) 508, 869
Alw26I GTCTC 2 cut(s) 193, 855
AlwI GGATC 3 cut(s) 396, 513, 526
Ama87I CYCGRG 2 cut(s) 297, 868
AoxI GGCC 3 cut(s) 19, 327, 758
ApeKI GCWGC 2 cut(s) 42, 877
ApoI RAATTY 4 cut(s) 374, 614, 653, 659
Asp700I GAANNNNTTC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 126, 773
AsuC2I CCSGG 3 cut(s) 231, 298, 299
AsuHPI GGTGA 5 cut(s) 112, 145, 175, 286, 849
AsuII TTCGAA 1 cut(s) 220
AvaI CYCGRG 2 cut(s) 297, 868
AvaII GGWCC 2 cut(s) 126, 773
BaeGI GKGCMC 1 cut(s) 57
BamHI GGATCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 236, 508
BbsI GAAGAC 1 cut(s) 667
Bbv12I GWGCWC 2 cut(s) 508, 869
BbvI GCAGC 2 cut(s) 54, 864
BccI CCATC 8 cut(s) 164, 208, 251, 400, 740, 862, 868, 896
BceAI ACGGC 1 cut(s) 817
BcgI CGANNNNNNTGC 4 cut(s) 811, 845, 859, 893
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 3 cut(s) 231, 298, 299
BcoDI GTCTC 2 cut(s) 193, 855
BfaI CTAG 2 cut(s) 590, 933
BfuAI ACCTGC 1 cut(s) 80
BisI GCNGC 2 cut(s) 43, 878
BlsI GCNGC 2 cut(s) 44, 879
Bme1390I CCNGG 4 cut(s) 124, 231, 298, 299
Bme18I GGWCC 2 cut(s) 126, 773
BmeT110I CYCGRG 2 cut(s) 297, 868
BmgT120I GGNCC 2 cut(s) 126, 773
BmiI GGNNCC 2 cut(s) 235, 520
BmrFI CCNGG 4 cut(s) 124, 231, 298, 299
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 282
BmuI ACTGGG 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 667
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 33
BpuMI CCSGG 3 cut(s) 231, 298, 299
BsaJI CCNNGG 2 cut(s) 297, 324
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 904, 934
Bse1I ACTGG 1 cut(s) 290
Bse3DI GCAATG 3 cut(s) 367, 631, 887
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 297, 324
BseGI GGATG 2 cut(s) 156, 676
BseMI GCAATG 3 cut(s) 367, 631, 887
BseMII CTCAG 2 cut(s) 101, 521
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 925
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 2 cut(s) 54, 864
BseYI CCCAGC 1 cut(s) 7
BshFI GGCC 3 cut(s) 21, 329, 760
BsiHKAI GWGCWC 2 cut(s) 508, 869
BsiHKCI CYCGRG 2 cut(s) 297, 868
BsiSI CCGG 3 cut(s) 230, 298, 761
BslFI GGGAC 1 cut(s) 302
BsmAI GTCTC 2 cut(s) 193, 855
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 3 cut(s) 21, 329, 760
BsoBI CYCGRG 2 cut(s) 297, 868
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 4 cut(s) 57, 236, 508, 869
Bsp143I GATC 4 cut(s) 3, 388, 413, 518
BspACI CCGC 3 cut(s) 321, 466, 645
BspANI GGCC 3 cut(s) 21, 329, 760
BspCNI CTCAG 2 cut(s) 100, 520
BspLI GGNNCC 2 cut(s) 235, 520
BspMI ACCTGC 1 cut(s) 80
BspPI GGATC 3 cut(s) 396, 513, 526
BspQI GCTCTTC 1 cut(s) 791
BspT104I TTCGAA 1 cut(s) 220
BsrDI GCAATG 3 cut(s) 367, 631, 887
BsrI ACTGG 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 297, 324
BssMI GATC 4 cut(s) 3, 388, 413, 518
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 4 cut(s) 187, 459, 716, 829
Bst6I CTCTTC 2 cut(s) 791, 977
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 2 cut(s) 23, 875
BstDEI CTNAG 2 cut(s) 87, 507
BstF5I GGATG 2 cut(s) 156, 676
BstKTI GATC 4 cut(s) 6, 391, 416, 521
BstMAI GTCTC 2 cut(s) 193, 855
BstMBI GATC 4 cut(s) 3, 388, 413, 518
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 15, 476
BstSCI CCNGG 4 cut(s) 122, 229, 296, 297
BstSLI GKGCMC 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 54, 864
BstV2I GAAGAC 1 cut(s) 667
BstX2I RGATCY 2 cut(s) 388, 518
BstYI RGATCY 2 cut(s) 388, 518
BsuRI GGCC 3 cut(s) 21, 329, 760
BtgZI GCGATG 2 cut(s) 331, 759
BtsCI GGATG 2 cut(s) 156, 676
BtsIMutI CAGTG 1 cut(s) 834
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 2 cut(s) 23, 875
Cfr13I GGNCC 2 cut(s) 126, 773
Cfr9I CCCGGG 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 122
Csp6I GTAC 1 cut(s) 555
CviAII CATG 7 cut(s) 12, 161, 336, 473, 719, 918, 939
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 2 cut(s) 87, 507
DpnI GATC 4 cut(s) 5, 390, 415, 520
DpnII GATC 4 cut(s) 3, 388, 413, 518
EaeI YGGCCR 2 cut(s) 19, 758
Eam1104I CTCTTC 2 cut(s) 791, 977
EarI CTCTTC 2 cut(s) 791, 977
EciI GGCGGA 2 cut(s) 310, 660
Ecl136II GAGCTC 1 cut(s) 506
Eco24I GRGCYC 2 cut(s) 236, 508
Eco47I GGWCC 2 cut(s) 126, 773
Eco53kI GAGCTC 1 cut(s) 506
Eco88I CYCGRG 2 cut(s) 297, 868
EcoICRI GAGCTC 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 773
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 122
EcoT38I GRGCYC 2 cut(s) 236, 508
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 7 cut(s) 15, 164, 339, 476, 722, 921, 942
FalI AAGNNNNNCTT 4 cut(s) 543, 575, 592, 624
FaqI GGGAC 1 cut(s) 302
FatI CATG 7 cut(s) 11, 160, 335, 472, 718, 917, 938
FauI CCCGC 1 cut(s) 459
FblI GTMKAC 2 cut(s) 573, 812
Fnu4HI GCNGC 2 cut(s) 43, 878
FokI GGATG 2 cut(s) 143, 683
FriOI GRGCYC 2 cut(s) 236, 508
Fsp4HI GCNGC 2 cut(s) 43, 878
FspBI CTAG 2 cut(s) 590, 933
GluI GCNGC 2 cut(s) 43, 878
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 3 cut(s) 21, 329, 760
HapII CCGG 3 cut(s) 230, 298, 761
Hin1II CATG 7 cut(s) 15, 164, 339, 476, 722, 921, 942
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HinfI GANTC 3 cut(s) 170, 332, 430
HpaII CCGG 3 cut(s) 230, 298, 761
HphI GGTGA 5 cut(s) 112, 145, 175, 286, 849
Hpy166II GTNNAC 3 cut(s) 557, 574, 813
Hpy188I TCNGA 3 cut(s) 169, 429, 620
Hpy188III TCNNGA 3 cut(s) 395, 501, 776
Hpy8I GTNNAC 3 cut(s) 557, 574, 813
HpyAV CCTTC 5 cut(s) 145, 190, 202, 217, 689
HpyCH4III ACNGT 4 cut(s) 187, 459, 716, 829
HpyCH4V TGCA 5 cut(s) 75, 96, 360, 365, 880
HpyF3I CTNAG 2 cut(s) 87, 507
Hsp92II CATG 7 cut(s) 15, 164, 339, 476, 722, 921, 942
Kzo9I GATC 4 cut(s) 3, 388, 413, 518
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 2 cut(s) 54, 864
LweI GCATC 1 cut(s) 282
MabI ACCWGGT 1 cut(s) 122
MaeI CTAG 2 cut(s) 590, 933
MalI GATC 4 cut(s) 5, 390, 415, 520
MboI GATC 4 cut(s) 3, 388, 413, 518
MboII GAAGA 6 cut(s) 130, 383, 643, 667, 808, 915
MfeI CAATTG 2 cut(s) 679, 837
MflI RGATCY 2 cut(s) 388, 518
MhlI GDGCHC 4 cut(s) 57, 236, 508, 869
MluCI AATT 8 cut(s) 374, 490, 531, 614, 653, 659, 679, 837
MlyI GAGTC 3 cut(s) 164, 341, 424
MmeI TCCRAC 1 cut(s) 452
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 862
MspI CCGG 3 cut(s) 230, 298, 761
MspR9I CCNGG 4 cut(s) 124, 231, 298, 299
MunI CAATTG 2 cut(s) 679, 837
MvaI CCWGG 1 cut(s) 124
NciI CCSGG 3 cut(s) 231, 298, 299
NdeII GATC 4 cut(s) 3, 388, 413, 518
NlaIII CATG 7 cut(s) 15, 164, 339, 476, 722, 921, 942
NlaIV GGNNCC 2 cut(s) 235, 520
NmeAIII GCCGAG 3 cut(s) 349, 355, 420
NspI RCATGY 2 cut(s) 15, 476
NspV TTCGAA 1 cut(s) 220
OliI CACNNNNGTG 1 cut(s) 862
PaeR7I CTCGAG 1 cut(s) 868
PciI ACATGT 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 818
PdmI GAANNNNTTC 1 cut(s) 378
PkrI GCNGC 2 cut(s) 44, 879
PleI GAGTC 3 cut(s) 164, 340, 424
PpsI GAGTC 3 cut(s) 164, 340, 424
PpuMI RGGWCCY 1 cut(s) 773
PscI ACATGT 1 cut(s) 472
Psp124BI GAGCTC 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 773
Psp6I CCWGG 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 235, 520
PspPI GGNCC 2 cut(s) 126, 773
PspPPI RGGWCCY 1 cut(s) 773
PspXI VCTCGAGB 1 cut(s) 868
PsuI RGATCY 2 cut(s) 388, 518
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 1 cut(s) 862
SacI GAGCTC 1 cut(s) 508
SalI GTCGAC 1 cut(s) 811
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 2 cut(s) 43, 878
Sau3AI GATC 4 cut(s) 3, 388, 413, 518
Sau96I GGNCC 2 cut(s) 126, 773
SchI GAGTC 3 cut(s) 164, 341, 424
ScrFI CCNGG 4 cut(s) 124, 231, 298, 299
SduI GDGCHC 4 cut(s) 57, 236, 508, 869
SexAI ACCWGGT 1 cut(s) 122
SfaNI GCATC 1 cut(s) 282
Sfr274I CTCGAG 1 cut(s) 868
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 2 cut(s) 126, 773
SlaI CTCGAG 1 cut(s) 868
SmaI CCCGGG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 862
SmlI CTYRAG 2 cut(s) 48, 868
SmoI CTYRAG 2 cut(s) 48, 868
Sse9I AATT 8 cut(s) 374, 490, 531, 614, 653, 659, 679, 837
SsiI CCGC 3 cut(s) 321, 466, 645
SspMI CTAG 2 cut(s) 590, 933
SstI GAGCTC 1 cut(s) 508
StyD4I CCNGG 4 cut(s) 122, 229, 296, 297
TaaI ACNGT 4 cut(s) 187, 459, 716, 829
TaqI TCGA 5 cut(s) 220, 562, 812, 821, 869
TasI AATT 8 cut(s) 374, 490, 531, 614, 653, 659, 679, 837
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 1 cut(s) 834
TseI GCWGC 2 cut(s) 42, 877
TspDTI ATGAA 3 cut(s) 708, 906, 915
TspGWI ACGGA 1 cut(s) 591
TspMI CCCGGG 1 cut(s) 297
TspRI CASTG 1 cut(s) 834
VpaK11BI GGWCC 2 cut(s) 126, 773
XapI RAATTY 4 cut(s) 374, 614, 653, 659
XceI RCATGY 2 cut(s) 15, 476
XhoI CTCGAG 1 cut(s) 868
XmaI CCCGGG 1 cut(s) 297
XmiI GTMKAC 2 cut(s) 573, 812
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 2 cut(s) 590, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.