MD04G1221400.v1.1

Protein tesmin TSO1-like CXC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0019245
Physical Location & Seq
Forward (+)
30271236 .. 30272513
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1221400.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGACACTCCTCTGAAGAACAATCAGACCACCCCCACCACACCTCTTCCTGCTAACTACGAGGATTCCCCTGTGTTCAACTACATTAGCAATCTTTCCCCAATTGAGCCGGTGAAGTCCGCAGGAAATGATCATCACACTTTCAACTCCCTAGCTTTTGCATCTCTTCCTTCTATTTTCGCTTCGCCGCAGAATCTCTCCATCCCCGATACCAGATTTGTACTTAGAAGGCACCACTTCTCTGATTCACCGAAACTTGAGTCCTTTCAGAGTGGAAATCGAAATAGTAAAAGTGATGCAGTTCCAGTTTCTGTTGAGCAATCGTATTTAGGTACTGACCAACCGGAATGCTTTGCATCCGGAAGTTCTTCAGATGCAGTACCAACTGAGAATTTGGAACTACCGGTTGAGTTTCTAAATACCTTAAAGTATAGTTCTGGTAGCCCACATAGCAATGATGTACATTCGAAAGGAAGGCACTCATTTTTTGAGAGGGAAGCGCAATTGTGCAGAATTCGCAGAGTTGAGCAGAAGAAAGACGCAGCCAGAGGCGATTTGGTTAGGTTGATTTCTGATGATCAGTTAAAATTTGATTCCACAACCATTAAAGAGAATCGTGCGGGGCACGATCCTAAGCCAGTAGATCCTGGAGAAATCTCTTTTATGTCAAAAATCCTACGAGACAATTGTTGGTATATGGTCCAGCCCATGATCAATGTTCTAGATTATTCTAGTAAGCAAGAGATGAGGCTGGAGCTTGCTAGACAATTCTAG

Protein Analysis

258

Amino Acids

28.92

Weight (kDa)

5.91

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019245)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03350 FvH4_6g03350
malus_domestica MD04G1221400.v1.1 MD04G1221500.v1.1 MD12G1237500.v1.1
prunus_persica Prupe.6G340600_v2.0.a1
pyrus_communis pycom04g19580 pycom12g21840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 359
AciI CCGC 3 cut(s) 120, 188, 620
AclWI GGATC 2 cut(s) 623, 638
AcsI RAATTY 3 cut(s) 391, 513, 587
AcuI CTGAAG 2 cut(s) 35, 354
AfaI GTAC 4 cut(s) 222, 334, 381, 462
AgeI ACCGGT 1 cut(s) 403
AgsI TTSAA 2 cut(s) 79, 145
AjnI CCWGG 1 cut(s) 646
AluBI AGCT 2 cut(s) 155, 757
AluI AGCT 2 cut(s) 155, 757
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 2 cut(s) 623, 638
AlwNI CAGNNNCTG 1 cut(s) 311
Aor13HI TCCGGA 1 cut(s) 359
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 3 cut(s) 391, 513, 587
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AsiGI ACCGGT 1 cut(s) 403
Asp700I GAANNNNTTC 1 cut(s) 367
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 1 cut(s) 700
AsuHPI GGTGA 2 cut(s) 124, 240
AsuII TTCGAA 1 cut(s) 467
AvaII GGWCC 1 cut(s) 700
BaeGI GKGCMC 1 cut(s) 627
BanI GGYRCC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 554
BccI CCATC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 648
BclI TGATCA 3 cut(s) 130, 577, 711
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 5 cut(s) 152, 722, 732, 762, 772
BisI GCNGC 2 cut(s) 188, 543
BlsI GCNGC 2 cut(s) 189, 544
Bme1390I CCNGG 1 cut(s) 648
Bme18I GGWCC 1 cut(s) 700
BmgT120I GGNCC 1 cut(s) 700
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 648
BmsI GCATC 4 cut(s) 170, 286, 364, 365
BpmI CTGGAG 2 cut(s) 669, 773
Bpu10I CCTNAGC 1 cut(s) 633
Bpu14I TTCGAA 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 278
BsaWI WCCGGW 3 cut(s) 343, 359, 403
Bse118I RCCGGY 2 cut(s) 109, 403
Bse1I ACTGG 2 cut(s) 305, 638
Bse3DI GCAATG 1 cut(s) 460
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 1 cut(s) 648
BseGI GGATG 2 cut(s) 201, 356
BseMI GCAATG 1 cut(s) 460
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 305, 638
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 1 cut(s) 554
BsgI GTGCAG 1 cut(s) 529
BshNI GGYRCC 1 cut(s) 231
BshTI ACCGGT 1 cut(s) 403
BsiSI CCGG 4 cut(s) 110, 344, 360, 404
BsmAI GTCTC 1 cut(s) 675
BsmI GAATGC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 627
Bsp13I TCCGGA 1 cut(s) 359
Bsp1407I TGTACA 1 cut(s) 460
Bsp143I GATC 5 cut(s) 130, 577, 628, 643, 711
BspACI CCGC 3 cut(s) 120, 188, 620
BspCNI CTCAG 1 cut(s) 379
BspEI TCCGGA 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 233
BspPI GGATC 2 cut(s) 623, 638
BspT104I TTCGAA 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 109, 403
BsrGI TGTACA 1 cut(s) 460
BsrI ACTGG 2 cut(s) 305, 638
BssAI RCCGGY 2 cut(s) 109, 403
BssMI GATC 5 cut(s) 130, 577, 628, 643, 711
Bst2UI CCWGG 1 cut(s) 648
Bst6I CTCTTC 2 cut(s) 51, 171
BstAUI TGTACA 1 cut(s) 460
BstBI TTCGAA 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 759
BstDEI CTNAG 3 cut(s) 224, 387, 633
BstF5I GGATG 2 cut(s) 201, 356
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 5 cut(s) 133, 580, 631, 646, 714
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 5 cut(s) 130, 577, 628, 643, 711
BstMWI GCNNNNNNNGC 2 cut(s) 450, 516
BstNI CCWGG 1 cut(s) 648
BstSCI CCNGG 1 cut(s) 646
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 1 cut(s) 554
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BtsCI GGATG 2 cut(s) 201, 356
Cac8I GCNNGC 1 cut(s) 759
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 2 cut(s) 109, 403
Cfr13I GGNCC 1 cut(s) 700
CseI GACGC 1 cut(s) 548
Csp6I GTAC 4 cut(s) 221, 333, 380, 461
CspAI ACCGGT 1 cut(s) 403
CviAII CATG 1 cut(s) 709
CviJI RGCY 8 cut(s) 109, 155, 444, 545, 637, 706, 751, 757
CviKI_1 RGCY 8 cut(s) 109, 155, 444, 545, 637, 706, 751, 757
CviQI GTAC 4 cut(s) 221, 333, 380, 461
DdeI CTNAG 3 cut(s) 224, 387, 633
DpnI GATC 5 cut(s) 132, 579, 630, 645, 713
DpnII GATC 5 cut(s) 130, 577, 628, 643, 711
Eam1104I CTCTTC 2 cut(s) 51, 171
EarI CTCTTC 2 cut(s) 51, 171
Eco47I GGWCC 1 cut(s) 700
Eco57I CTGAAG 2 cut(s) 35, 354
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 646
FaeI CATG 1 cut(s) 712
FaiI YATR 6 cut(s) 432, 450, 665, 696, 698, 710
FatI CATG 1 cut(s) 708
FauI CCCGC 1 cut(s) 613
FbaI TGATCA 3 cut(s) 130, 577, 711
Fnu4HI GCNGC 2 cut(s) 188, 543
FokI GGATG 2 cut(s) 188, 343
Fsp4HI GCNGC 2 cut(s) 188, 543
FspBI CTAG 5 cut(s) 152, 722, 732, 762, 772
GlaI GCGC 1 cut(s) 501
GluI GCNGC 2 cut(s) 188, 543
GsuI CTGGAG 2 cut(s) 669, 773
HapII CCGG 4 cut(s) 110, 344, 360, 404
HgaI GACGC 1 cut(s) 548
HhaI GCGC 1 cut(s) 502
Hin1II CATG 1 cut(s) 712
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 6 cut(s) 65, 193, 245, 260, 593, 613
HpaII CCGG 4 cut(s) 110, 344, 360, 404
HphI GGTGA 2 cut(s) 124, 240
Hpy188I TCNGA 6 cut(s) 15, 27, 244, 270, 373, 574
Hpy188III TCNNGA 2 cut(s) 360, 722
HpyAV CCTTC 3 cut(s) 180, 222, 468
HpyCH4V TGCA 5 cut(s) 161, 299, 356, 377, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 450, 516
HpyF3I CTNAG 3 cut(s) 224, 387, 633
Hsp92II CATG 1 cut(s) 712
HspAI GCGC 1 cut(s) 500
Kpn2I TCCGGA 1 cut(s) 359
Ksp22I TGATCA 3 cut(s) 130, 577, 711
Kzo9I GATC 5 cut(s) 130, 577, 628, 643, 711
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 1 cut(s) 554
LweI GCATC 4 cut(s) 170, 286, 364, 365
MaeI CTAG 5 cut(s) 152, 722, 732, 762, 772
MalI GATC 5 cut(s) 132, 579, 630, 645, 713
MboI GATC 5 cut(s) 130, 577, 628, 643, 711
MboII GAAGA 5 cut(s) 28, 38, 158, 360, 544
MfeI CAATTG 3 cut(s) 102, 503, 685
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 7 cut(s) 102, 391, 503, 513, 587, 685, 767
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 6 cut(s) 21, 54, 55, 486, 542, 741
MroI TCCGGA 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 3 cut(s) 425, 584, 606
MslI CAYNNNNRTG 1 cut(s) 453
MspI CCGG 4 cut(s) 110, 344, 360, 404
MspR9I CCNGG 1 cut(s) 648
MunI CAATTG 3 cut(s) 102, 503, 685
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 1 cut(s) 648
MwoI GCNNNNNNNGC 2 cut(s) 450, 516
NdeII GATC 5 cut(s) 130, 577, 628, 643, 711
NlaIII CATG 1 cut(s) 712
NlaIV GGNNCC 1 cut(s) 233
NspV TTCGAA 1 cut(s) 467
PctI GAATGC 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 5 cut(s) 65, 193, 245, 593, 613
PfoI TCCNGGA 1 cut(s) 646
PinAI ACCGGT 1 cut(s) 403
PkrI GCNGC 2 cut(s) 189, 544
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 646
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 1 cut(s) 233
PspPI GGNCC 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 643
RsaI GTAC 4 cut(s) 222, 334, 381, 462
RsaNI GTAC 4 cut(s) 221, 333, 380, 461
RseI CAYNNNNRTG 1 cut(s) 453
SaqAI TTAA 3 cut(s) 425, 584, 606
SatI GCNGC 2 cut(s) 188, 543
Sau3AI GATC 5 cut(s) 130, 577, 628, 643, 711
Sau96I GGNCC 1 cut(s) 700
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 648
SduI GDGCHC 1 cut(s) 627
SetI ASST 6 cut(s) 46, 157, 334, 425, 566, 759
SfaNI GCATC 4 cut(s) 170, 286, 364, 365
SfuI TTCGAA 1 cut(s) 467
SinI GGWCC 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 7 cut(s) 102, 391, 503, 513, 587, 685, 767
SsiI CCGC 3 cut(s) 120, 188, 620
SspMI CTAG 5 cut(s) 152, 722, 732, 762, 772
StyD4I CCNGG 1 cut(s) 646
TaqI TCGA 2 cut(s) 280, 467
TasI AATT 7 cut(s) 102, 391, 503, 513, 587, 685, 767
TatI WGTACW 2 cut(s) 220, 460
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 5 cut(s) 65, 193, 245, 593, 613
Tru1I TTAA 3 cut(s) 425, 584, 606
Tru9I TTAA 3 cut(s) 425, 584, 606
TseI GCWGC 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 700
XapI RAATTY 3 cut(s) 391, 513, 587
XbaI TCTAGA 1 cut(s) 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 367
XspI CTAG 5 cut(s) 152, 722, 732, 762, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.