Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0019245

Physical Location & Seq

Genomic Coordinates Forward (+)

31113648 .. 31117687

Gene Structure 4040 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1237500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGGACACTCCGCTGAAGAATCAGATCACCCCCACCACTCCTCTTCCTGCTAAATACGAGGATTCCCCTGTGTTCAACTACATTAGCAATCTTTCCCCGATTGAGCCGGTGAAGTCCGTAGGAAATGATCATCACACTTTCAACTCCCTAGCTTTTGCATCTCTTCCTTCTATTTTCGCTTCGCCGCAGAATCTTTCCGTCCCGGAAACACGGTTCTTACTTAGAAGGCATCACTTCTCTGATTCACCGAAACCCGAGTCCTTCCAGAGTGGAAACCGAAACAGTAAAAGTGATGGAGTTCCGGTTTCCGTTGAGCAAGCGTATTTAGGTACTGACCAACCGGAATGCTTTGCATCCGGAAGTTCTTCAGATGTAGAGGTGGCTAGTGCAGTACCAACTGAGAATTTGGAACCACCGGTTGAGTTTCTAAATACCTTGAAGTATAGTTCTGGTAGCCCACATAGAAATGATGTACATCATTCGAAAGGAAGGCACTCGTTTTTCGAGAGGGAAACACATTTGCGCAGAATTCGAAGAGTTGAGCAGAAGAAAGATGCAGCCGCATGCGATTTGGTGAGGTTGATTTCTGATGATCAGTTAAAATTTGATTTGACAACCATTAAAGAGAATTGTGCGGGGCACGATCCTAAACCAGTAGATCCTGGAGAAATCTCTTTTATGTCAAACATCCTACGAGACAATGATATTGAAGTAGAGGAATCTGCTGGTCCTATTGATTCTTGTGAAGAATGCGAATTGGGAAAGGTCAGTAATCAACCAGAAGGTGTTCGGGGAACTAAGGAAACAGATCAAGCTCCGGCGATACTTTCTGAAACTTTACTGGATAAGTTAAATGTTAATGATCCAAGTGGTATTGTGGATGAAAAGTCGCTGAATTGTATACATTCTAGCTGCAAGCCTAGTTCTCAGTCATATGCAATACGCAGGCGCTGTTTGGATTTTGAAAGGCCAGCAGCTCACAAAAGGAAATCAATAGATGCTAGTGGCGGTTCTTTAGCCTTACCGCAGTCCAGTTGTGAAGTCGCCTCTGTCGAAAAGCAGTTGGTTCAAACTATAAAAGAAACTCCTCCCCAAGTATGTGCAGCAGTTGGTCAAGAACATGACTGTAATAGTCCTGAGAAGAAAAGGCTTAAGTTGCAGCCTGTTGGAGAAAGTTTGGCTTGCAAGCGCTGTAATTGTAAGAGATCAAAATGTTTGAAACTTTATTGCGACTGTTTTGCTGCTGGTCTCTACTGTATTGAGCCTTGTTCATGTCAAGAATGCTTAAACAAGCCTAATTATGAGAATATTGTTTTAGAGAATCGCAAACTGATTGAATCTCGCAACCCACTTGCATTTGCTCCCAAAGTAACTGCAAGCGCCGTTGCTATTCCACAGTACGGGAAGGAAACTAACTCGACTCCGTCTTCAGCTAGGCATAAAAGGGGATGCAATTGTAGAAAATCAAGTTGCTTGAAAAAGTACTGTGAATGCTTCCAGGGTGGTGCTGGATGCGGCATCCTCTGCAGATGTGTAGGGTGTAAAAACACTTTTGGCCGGAAAGATGAATCTAAACAAGCTGAAGTTGGAGGAGATGAATCAGTAGTTCCGAATAAGGATGCACTGAATGTCAGTTTACCGACAGTAAGGGATCAAGATCTCCCAATGGCGCGTTCTGGGATTTTCAGACCTCCAGACCAATTGACATCCTCTTCTAACGGGAAACAGAAAATATTTTCTCTTCATTCTGTGGTATCATCTCCTCAGCTCGAGAAGCATCTTCAGGCAATTCCTGAAGACGGAACTCTTGAGACGTTGGCTAGCAGTGGCTCACATAAGTCATCCTCTCCAAACTCAAAGAGGGTATCGCCTCCTCACAGCGGCAGCGAGTTTGGGTCAGCTTGGAGGGGCGGCAGGAAGTTAGTTCTGAGATCCATTCCACCATTCCCGACTCTGGAATCACCACGAAAGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

TCR

Protein Analysis

659

Amino Acids

71.99

Weight (kDa)

8.0

Isoelectric Point (pI)

67.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TCR	PF03638	396 - 431	8.9e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain
TCR	PF03638	481 - 517	7.8e-12	Tesmin/TSO1-like CXC domain, cysteine-rich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019245)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g03350 FvH4_6g03350
malus_domestica	MD04G1221400.v1.1 MD04G1221500.v1.1 MD12G1237500.v1.1
prunus_persica	Prupe.6G340600_v2.0.a1
pyrus_communis	pycom04g19580 pycom12g21840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	524
AccI	GTMKAC	1 cut(s)	901
AccII	CGCG	1 cut(s)	1672
AccIII	TCCGGA	1 cut(s)	356
AciI	CCGC	9 cut(s)	11, 185, 561, 635, 1008, 1025, 1515, 1881, 1911
AclWI	GGATC	5 cut(s)	638, 653, 857, 1659, 1926
AcoI	YGGCCR	1 cut(s)	1555
AcsI	RAATTY	3 cut(s)	403, 528, 602
AcuI	CTGAAG	6 cut(s)	35, 351, 1413, 1602, 1766, 1815
AfaI	GTAC	5 cut(s)	331, 393, 474, 1400, 1484
AfeI	AGCGCT	1 cut(s)	1190
AfiI	CCNNNNNNNGG	4 cut(s)	1400, 1799, 1880, 1954
AflII	CTTAAG	1 cut(s)	1151
AgeI	ACCGGT	1 cut(s)	415
AgsI	TTSAA	9 cut(s)	76, 142, 439, 710, 965, 1070, 1219, 1337, 1477
AjnI	CCWGG	2 cut(s)	661, 1497
AjuI	GAANNNNNNNTTGG	2 cut(s)	1657, 1689
AloI	GAACNNNNNNTCC	2 cut(s)	197, 229
AluBI	AGCT	8 cut(s)	152, 815, 912, 977, 1433, 1580, 1768, 1901
AluI	AGCT	8 cut(s)	152, 815, 912, 977, 1433, 1580, 1768, 1901
Alw26I	GTCTC	3 cut(s)	690, 1253, 1805
AlwI	GGATC	5 cut(s)	638, 653, 857, 1659, 1926
AlwNI	CAGNNNCTG	1 cut(s)	951
Ama87I	CYCGRG	2 cut(s)	254, 1769
Aor13HI	TCCGGA	1 cut(s)	356
Aor51HI	AGCGCT	1 cut(s)	1190
AoxI	GGCC	2 cut(s)	968, 1555
ApeKI	GCWGC	7 cut(s)	557, 912, 974, 1103, 1159, 1241, 1884
ApoI	RAATTY	3 cut(s)	403, 528, 602
ArsI	GACNNNNNNTTYG	2 cut(s)	242, 274
AsiGI	ACCGGT	1 cut(s)	415
Asp700I	GAANNNNTTC	2 cut(s)	364, 786
AspLEI	GCGC	5 cut(s)	525, 951, 1191, 1382, 1672
AspS9I	GGNCC	1 cut(s)	728
AsuC2I	CCSGG	1 cut(s)	203
AsuHPI	GGTGA	5 cut(s)	19, 121, 237, 586, 1953
AsuII	TTCGAA	2 cut(s)	482, 532
AsuNHI	GCTAGC	1 cut(s)	1820
AvaI	CYCGRG	2 cut(s)	254, 1769
AvaII	GGWCC	1 cut(s)	728
BaeGI	GKGCMC	1 cut(s)	642
BbsI	GAAGAC	2 cut(s)	1419, 1803
BbvCI	CCTCAGC	1 cut(s)	1764
BbvI	GCAGC	7 cut(s)	569, 899, 986, 1115, 1171, 1228, 1896
BccI	CCATC	1 cut(s)	287
BceAI	ACGGC	1 cut(s)	1367
BcgI	CGANNNNNNTGC	2 cut(s)	1220, 1254
BciT130I	CCWGG	2 cut(s)	663, 1499
BclI	TGATCA	2 cut(s)	127, 592
BcnI	CCSGG	1 cut(s)	203
BcoDI	GTCTC	3 cut(s)	690, 1253, 1805
BfaI	CTAG	7 cut(s)	149, 384, 909, 921, 1002, 1434, 1821
BfmI	CTRYAG	1 cut(s)	1525
BfoI	RGCGCY	3 cut(s)	952, 1192, 1383
BfrI	CTTAAG	1 cut(s)	1151
BglII	AGATCT	1 cut(s)	1657
BmcAI	AGTACT	1 cut(s)	1484
Bme1390I	CCNGG	3 cut(s)	203, 663, 1499
Bme18I	GGWCC	1 cut(s)	728
BmeT110I	CYCGRG	2 cut(s)	254, 1769
BmgT120I	GGNCC	1 cut(s)	728
BmiI	GGNNCC	1 cut(s)	411
BmrFI	CCNGG	3 cut(s)	203, 663, 1499
BmtI	GCTAGC	1 cut(s)	1824
BpiI	GAAGAC	2 cut(s)	1419, 1803
BpmI	CTGGAG	2 cut(s)	684, 1677
Bpu10I	CCTNAGC	1 cut(s)	1764
Bpu14I	TTCGAA	2 cut(s)	482, 532
BpuEI	CTTGAG	1 cut(s)	1829
BpuMI	CCSGG	1 cut(s)	203
BsaBI	GATNNNNATC	2 cut(s)	474, 1656
BsaI	GGTCTC	1 cut(s)	1253
BsaJI	CCNNGG	1 cut(s)	1498
BsaWI	WCCGGW	4 cut(s)	301, 340, 356, 415
BsaXI	ACNNNNNCTCC	2 cut(s)	288, 318
Bsc4I	CCNNNNNNNGG	4 cut(s)	1400, 1799, 1880, 1954
Bse118I	RCCGGY	2 cut(s)	106, 415
Bse1I	ACTGG	3 cut(s)	653, 846, 1032
Bse8I	GATNNNNATC	2 cut(s)	474, 1656
BseAI	TCCGGA	1 cut(s)	356
BseBI	CCWGG	2 cut(s)	663, 1499
BseDI	CCNNGG	1 cut(s)	1498
BseGI	GGATG	9 cut(s)	353, 687, 886, 1454, 1517, 1518, 1624, 1706, 1841
BseJI	GATNNNNATC	2 cut(s)	474, 1656
BseLI	CCNNNNNNNGG	4 cut(s)	1400, 1799, 1880, 1954
BseMII	CTCAG	5 cut(s)	390, 941, 1128, 1778, 1919
BseNI	ACTGG	3 cut(s)	653, 846, 1032
BseRI	GAGGAG	5 cut(s)	30, 1077, 1605, 1752, 1863
BseSI	GKGCMC	1 cut(s)	642
BseXI	GCAGC	7 cut(s)	569, 899, 986, 1115, 1171, 1228, 1896
BsgI	GTGCAG	2 cut(s)	408, 1122
Bsh1236I	CGCG	1 cut(s)	1672
BshFI	GGCC	2 cut(s)	970, 1557
BshTI	ACCGGT	1 cut(s)	415
BsiHKCI	CYCGRG	2 cut(s)	254, 1769
BsiSI	CCGG	8 cut(s)	107, 203, 302, 341, 357, 416, 818, 1558
BslFI	GGGAC	1 cut(s)	185
BslI	CCNNNNNNNGG	4 cut(s)	1400, 1799, 1880, 1954
BsmAI	GTCTC	3 cut(s)	690, 1253, 1805
BsmBI	CGTCTC	1 cut(s)	1805
BsmFI	GGGAC	1 cut(s)	185
BsmI	GAATGC	4 cut(s)	350, 755, 1286, 1496
BsnI	GGCC	2 cut(s)	970, 1557
Bso31I	GGTCTC	1 cut(s)	1253
BsoBI	CYCGRG	2 cut(s)	254, 1769
Bsp119I	TTCGAA	2 cut(s)	482, 532
Bsp1286I	GDGCHC	1 cut(s)	642
Bsp13I	TCCGGA	1 cut(s)	356
Bsp1407I	TGTACA	1 cut(s)	472
BspACI	CCGC	9 cut(s)	11, 185, 561, 635, 1008, 1025, 1515, 1881, 1911
BspANI	GGCC	2 cut(s)	970, 1557
BspCNI	CTCAG	5 cut(s)	391, 940, 1129, 1777, 1920
BspEI	TCCGGA	1 cut(s)	356
BspFNI	CGCG	1 cut(s)	1672
BspLI	GGNNCC	1 cut(s)	411
BspMAI	CTGCAG	1 cut(s)	1529
BspOI	GCTAGC	1 cut(s)	1824
BspPI	GGATC	5 cut(s)	638, 653, 857, 1659, 1926
BspT104I	TTCGAA	2 cut(s)	482, 532
BspTI	CTTAAG	1 cut(s)	1151
BspTNI	GGTCTC	1 cut(s)	1253
BsrFI	RCCGGY	2 cut(s)	106, 415
BsrGI	TGTACA	1 cut(s)	472
BsrI	ACTGG	3 cut(s)	653, 846, 1032
BssAI	RCCGGY	2 cut(s)	106, 415
BssECI	CCNNGG	1 cut(s)	1498
BssNAI	GTATAC	1 cut(s)	902
Bst1107I	GTATAC	1 cut(s)	902
Bst2UI	CCWGG	2 cut(s)	663, 1499
Bst4CI	ACNGT	8 cut(s)	213, 284, 1127, 1235, 1256, 1398, 1487, 1645
Bst6I	CTCTTC	5 cut(s)	48, 168, 529, 1717, 1746
BstAFI	CTTAAG	1 cut(s)	1151
BstAPI	GCANNNNNTGC	1 cut(s)	1524
BstAUI	TGTACA	1 cut(s)	472
BstBI	TTCGAA	2 cut(s)	482, 532
BstC8I	GCNNGC	9 cut(s)	318, 565, 917, 947, 972, 1183, 1187, 1378, 1822
BstDEI	CTNAG	7 cut(s)	221, 399, 798, 927, 1137, 1764, 1928
BstF5I	GGATG	9 cut(s)	353, 687, 886, 1454, 1517, 1518, 1624, 1706, 1841
BstFNI	CGCG	1 cut(s)	1672
BstH2I	RGCGCY	3 cut(s)	952, 1192, 1383
BstHHI	GCGC	5 cut(s)	525, 951, 1191, 1382, 1672
BstMAI	GTCTC	3 cut(s)	690, 1253, 1805
BstMWI	GCNNNNNNNGC	3 cut(s)	1156, 1524, 1774
BstNI	CCWGG	2 cut(s)	663, 1499
BstNSI	RCATGY	1 cut(s)	567
BstSCI	CCNGG	3 cut(s)	201, 661, 1497
BstSFI	CTRYAG	1 cut(s)	1525
BstSLI	GKGCMC	1 cut(s)	642
BstUI	CGCG	1 cut(s)	1672
BstV1I	GCAGC	7 cut(s)	569, 899, 986, 1115, 1171, 1228, 1896
BstV2I	GAAGAC	2 cut(s)	1419, 1803
BstX2I	RGATCY	3 cut(s)	658, 1657, 1931
BstYI	RGATCY	3 cut(s)	658, 1657, 1931
BstZ17I	GTATAC	1 cut(s)	902
BsuRI	GGCC	2 cut(s)	970, 1557
BtsCI	GGATG	9 cut(s)	353, 687, 886, 1454, 1517, 1518, 1624, 1706, 1841
BtsI	GCAGTG	1 cut(s)	1831
BtsIMutI	CAGTG	2 cut(s)	1622, 1831
Cac8I	GCNNGC	9 cut(s)	318, 565, 917, 947, 972, 1183, 1187, 1378, 1822
CaiI	CAGNNNCTG	1 cut(s)	951
CfoI	GCGC	5 cut(s)	525, 951, 1191, 1382, 1672
Cfr10I	RCCGGY	2 cut(s)	106, 415
Cfr13I	GGNCC	1 cut(s)	728
Csp6I	GTAC	5 cut(s)	330, 392, 473, 1399, 1483
CspAI	ACCGGT	1 cut(s)	415
CviAII	CATG	3 cut(s)	564, 1121, 1272
CviQI	GTAC	5 cut(s)	330, 392, 473, 1399, 1483
DdeI	CTNAG	7 cut(s)	221, 399, 798, 927, 1137, 1764, 1928
EaeI	YGGCCR	1 cut(s)	1555
Eam1104I	CTCTTC	5 cut(s)	48, 168, 529, 1717, 1746
EarI	CTCTTC	5 cut(s)	48, 168, 529, 1717, 1746
Eco31I	GGTCTC	1 cut(s)	1253
Eco47I	GGWCC	1 cut(s)	728
Eco47III	AGCGCT	1 cut(s)	1190
Eco57I	CTGAAG	6 cut(s)	35, 351, 1413, 1602, 1766, 1815
Eco88I	CYCGRG	2 cut(s)	254, 1769
EcoRI	GAATTC	1 cut(s)	528
EcoRII	CCWGG	2 cut(s)	661, 1497
Esp3I	CGTCTC	1 cut(s)	1805
FaeI	CATG	3 cut(s)	567, 1124, 1275
FalI	AAGNNNNNCTT	2 cut(s)	1165, 1197
FaqI	GGGAC	1 cut(s)	185
FatI	CATG	3 cut(s)	563, 1120, 1271
FauI	CCCGC	1 cut(s)	628
FauNDI	CATATG	1 cut(s)	934
FbaI	TGATCA	2 cut(s)	127, 592
FblI	GTMKAC	1 cut(s)	901
FokI	GGATG	9 cut(s)	340, 674, 893, 1461, 1505, 1524, 1631, 1693, 1828
FspBI	CTAG	7 cut(s)	149, 384, 909, 921, 1002, 1434, 1821
FspI	TGCGCA	1 cut(s)	524
GlaI	GCGC	5 cut(s)	524, 950, 1190, 1381, 1671
GsuI	CTGGAG	2 cut(s)	684, 1677
HaeII	RGCGCY	3 cut(s)	952, 1192, 1383
HaeIII	GGCC	2 cut(s)	970, 1557
HapII	CCGG	8 cut(s)	107, 203, 302, 341, 357, 416, 818, 1558
HhaI	GCGC	5 cut(s)	525, 951, 1191, 1382, 1672
Hin1II	CATG	3 cut(s)	567, 1124, 1275
Hin6I	GCGC	5 cut(s)	523, 949, 1189, 1380, 1670
HinP1I	GCGC	5 cut(s)	523, 949, 1189, 1380, 1670
HpaII	CCGG	8 cut(s)	107, 203, 302, 341, 357, 416, 818, 1558
HphI	GGTGA	5 cut(s)	19, 121, 237, 586, 1953
Hpy166II	GTNNAC	2 cut(s)	902, 1637
Hpy188I	TCNGA	8 cut(s)	24, 241, 370, 589, 832, 1611, 1688, 1929
Hpy8I	GTNNAC	2 cut(s)	902, 1637
HpyAV	CCTTC	6 cut(s)	177, 219, 271, 483, 776, 1399
HpyCH4III	ACNGT	8 cut(s)	213, 284, 1127, 1235, 1256, 1398, 1487, 1645
HpyCH4IV	ACGT	1 cut(s)	1814
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1156, 1524, 1774
HpyF3I	CTNAG	7 cut(s)	221, 399, 798, 927, 1137, 1764, 1928
HpySE526I	ACGT	1 cut(s)	1814
Hsp92II	CATG	3 cut(s)	567, 1124, 1275
HspAI	GCGC	5 cut(s)	523, 949, 1189, 1380, 1670
Kpn2I	TCCGGA	1 cut(s)	356
Ksp22I	TGATCA	2 cut(s)	127, 592
LmnI	GCTCC	2 cut(s)	820, 1366
Lsp1109I	GCAGC	7 cut(s)	569, 899, 986, 1115, 1171, 1228, 1896
MaeI	CTAG	7 cut(s)	149, 384, 909, 921, 1002, 1434, 1821
MaeII	ACGT	1 cut(s)	1814
MaeIII	GTNAC	1 cut(s)	1369
MfeI	CAATTG	2 cut(s)	1453, 1700
MflI	RGATCY	3 cut(s)	658, 1657, 1931
MhlI	GDGCHC	1 cut(s)	642
MlyI	GAGTC	3 cut(s)	266, 1414, 1945
MmeI	TCCRAC	2 cut(s)	1147, 1567
MroI	TCCGGA	1 cut(s)	356
MroXI	GAANNNNTTC	2 cut(s)	364, 786
MseI	TTAA	6 cut(s)	599, 621, 851, 858, 1152, 1286
MslI	CAYNNNNRTG	1 cut(s)	465
MspA1I	CMGCKG	2 cut(s)	13, 1881
MspCI	CTTAAG	1 cut(s)	1151
MspI	CCGG	8 cut(s)	107, 203, 302, 341, 357, 416, 818, 1558
MspR9I	CCNGG	3 cut(s)	203, 663, 1499
MunI	CAATTG	2 cut(s)	1453, 1700
Mva1269I	GAATGC	4 cut(s)	350, 755, 1286, 1496
MvaI	CCWGG	2 cut(s)	663, 1499
MvnI	CGCG	1 cut(s)	1672
MwoI	GCNNNNNNNGC	3 cut(s)	1156, 1524, 1774
NciI	CCSGG	1 cut(s)	203
NdeI	CATATG	1 cut(s)	934
NheI	GCTAGC	1 cut(s)	1820
NlaIII	CATG	3 cut(s)	567, 1124, 1275
NlaIV	GGNNCC	1 cut(s)	411
NsbI	TGCGCA	1 cut(s)	524
NspI	RCATGY	1 cut(s)	567
NspV	TTCGAA	2 cut(s)	482, 532
PaeI	GCATGC	1 cut(s)	567
PaeR7I	CTCGAG	1 cut(s)	1769
PcsI	WCGNNNNNNNCGW	1 cut(s)	1050
PctI	GAATGC	4 cut(s)	350, 755, 1286, 1496
PdmI	GAANNNNTTC	2 cut(s)	364, 786
PflFI	GACNNNGTC	1 cut(s)	1423
PfoI	TCCNGGA	2 cut(s)	201, 661
PinAI	ACCGGT	1 cut(s)	415
PleI	GAGTC	3 cut(s)	265, 1414, 1945
PpsI	GAGTC	3 cut(s)	265, 1414, 1945
Psp6I	CCWGG	2 cut(s)	661, 1497
PspGI	CCWGG	2 cut(s)	661, 1497
PspN4I	GGNNCC	1 cut(s)	411
PspPI	GGNCC	1 cut(s)	728
PstI	CTGCAG	1 cut(s)	1529
PstNI	CAGNNNCTG	1 cut(s)	951
PsuI	RGATCY	3 cut(s)	658, 1657, 1931
PsyI	GACNNNGTC	1 cut(s)	1423
RsaI	GTAC	5 cut(s)	331, 393, 474, 1400, 1484
RsaNI	GTAC	5 cut(s)	330, 392, 473, 1399, 1483
RseI	CAYNNNNRTG	1 cut(s)	465
SaqAI	TTAA	6 cut(s)	599, 621, 851, 858, 1152, 1286
Sau96I	GGNCC	1 cut(s)	728
ScaI	AGTACT	1 cut(s)	1484
SchI	GAGTC	3 cut(s)	266, 1414, 1945
ScrFI	CCNGG	3 cut(s)	203, 663, 1499
SduI	GDGCHC	1 cut(s)	642
SfcI	CTRYAG	1 cut(s)	1525
Sfr274I	CTCGAG	1 cut(s)	1769
SfuI	TTCGAA	2 cut(s)	482, 532
SinI	GGWCC	1 cut(s)	728
SlaI	CTCGAG	1 cut(s)	1769
SmiMI	CAYNNNNRTG	1 cut(s)	465
SmlI	CTYRAG	3 cut(s)	1151, 1769, 1808
SmoI	CTYRAG	3 cut(s)	1151, 1769, 1808
SphI	GCATGC	1 cut(s)	567
SsiI	CCGC	9 cut(s)	11, 185, 561, 635, 1008, 1025, 1515, 1881, 1911
SspI	AATATT	2 cut(s)	1309, 1734
SspMI	CTAG	7 cut(s)	149, 384, 909, 921, 1002, 1434, 1821
StyD4I	CCNGG	3 cut(s)	201, 661, 1497
TaaI	ACNGT	8 cut(s)	213, 284, 1127, 1235, 1256, 1398, 1487, 1645
TaiI	ACGT	1 cut(s)	1817
TaqI	TCGA	6 cut(s)	482, 504, 532, 1053, 1418, 1770
TatI	WGTACW	2 cut(s)	472, 1482
TauI	GCSGC	5 cut(s)	187, 563, 1518, 1884, 1914
Tru1I	TTAA	6 cut(s)	599, 621, 851, 858, 1152, 1286
Tru9I	TTAA	6 cut(s)	599, 621, 851, 858, 1152, 1286
TscAI	CASTG	2 cut(s)	1629, 1831
TseI	GCWGC	7 cut(s)	557, 912, 974, 1103, 1159, 1241, 1884
TspDTI	ATGAA	5 cut(s)	897, 1260, 1581, 1611, 1733
TspGWI	ACGGA	5 cut(s)	106, 187, 298, 1413, 1815
TspRI	CASTG	2 cut(s)	1629, 1831
Tth111I	GACNNNGTC	1 cut(s)	1423
Vha464I	CTTAAG	1 cut(s)	1151
VpaK11BI	GGWCC	1 cut(s)	728
XapI	RAATTY	3 cut(s)	403, 528, 602
XceI	RCATGY	1 cut(s)	567
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1505
XhoI	CTCGAG	1 cut(s)	1769
XmiI	GTMKAC	1 cut(s)	901
XmnI	GAANNNNTTC	2 cut(s)	364, 786
XspI	CTAG	7 cut(s)	149, 384, 909, 921, 1002, 1434, 1821
ZrmI	AGTACT	1 cut(s)	1484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.