pycom12g21840

tesmin TSO1-like CXC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0019245
Physical Location & Seq
Forward (+)
22961441 .. 22963982
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21840.1

Sequence Viewer

Length: 1782 bp
ATGGACACTCCGCTGAAGAATCAGATCACCCCCACCACTCCTCTTCCTGCTAAATACGAGGATTCCCCTGTGTTCAACTACATTAGCAATCTTTCCCCAATTGAGCCGGTGAACTCCGCAGGAAACGATCATCACACTTTCAACTCCCTAGCTTTTGCATCTCTTCCTTCTATTTTCGCTTCGCCGCAGAATCTTTCCGTCCCAGAAACACAGTTCTTACTTAGAAGGCAGCACTTCTCTGATTCACCGAAACCCGAGTCCTTCCAGAGTGGAAACCGAAACAGTAAAAGTGATGGAGTTCCGGTTTCTGTTGAGCAAGCGTATTTAGGTACTGACCAACCGGAATGCTTTGCATCCGGAAGTTCTTCAGATGTAGAGGTAGCTAGTGCAGTACCAACTGAGAATTTGGAACCACCGGTTGAGTTTCTAAATACCTTGAAGTATAGTTCAGGTAGCCCACATAGAAATGATGTACATCATTCGAAAGGAAGGCACTCGCTTTTCGAGAGGGATACACATTTGCGCAGAATTCTGAGAGTTGAGCAGAAGAAAGATGCAGCCGCATGCGATCTGGTGAGGTTGATTTCTGATGATCAGTTAAAATTTGATTTGACAACCATTAAAGAGAAATGTGCGAGGCACGATCCTAAACCAGTAGATCCTGGCGAAATCTCTTTTATGTCAAACATCCTACGAGACAATGATATTGAAGTAGAGGAATCTGCTGGTCCTATCGATTCTAGTGAAGAAAGCGAATTGGGAAAGGTCAGTAATCAACCAGAAGGTATTCGGGGAACTAAGGAAACAGATCAAGCTCCGGCGATACTTTCTGGAACTTTACTGGATAAGTTAAATGTTAATGATCCAAGTGGTATTGTGGATAAAAAGTCGCTGAATTGTATACATTCTAGCTGCAAGCCTAGTTCTCAGTCATACGCAATACGCAGGCGCTGTTTGGATTTTGAAGGGCCAGCAGCTCACCAAAGGAAATCAATAGATGCTAGTGGCAGTTCTTTAGCCTTACCGCAGTCCAGTTGTGAAGTCGCCTCTGTCGAAAAGCAGTTGGTTCAAACTATAAAAGGTTGTGATTATTCATCTTCAAGGTTACCTGGCATTGGTTTGCACTTGAATGCTCTTGCAACTTCTAGTGAAAGTAACTTAAGTAAGGTTGTCAAGCATGATATTTCTGAGAGTCGAGCGACAAATTTTCCAAACTCCAAGATATCTAGTACTTCTTTGACTCCCAGTGAGGTTTCTCGTGATGAATCATGTAAAAATAAAGCTCAGGTTGCAGAAACTCCTCCCCAAGTATGTGCAGCAGTTGGTCAAGAACATGACTGTAATAGTCCTGAGAAGAAAAGGCATGCGCTTAGGTTGCAGCCTGTTGGAGAAAGTTTGGCTTGCAAGCGCTGCAACTGTAAGAGATCAAAGTGTTTGAAACTTTATTGCGATTGTTTTGCTGCTGGTCTCTACTGTATTGAGCCTTGTTCATGTCAAGAATGCTTCAACAAGCCTAATTATGAGAATATTGTTTTAGAGAATCGCAAACTGATTGAATCTCGCAACCCACTTGCATTTGCTCCCAAAGTAACTGCAAGTGCCGTTGCTATTCCACAGCACGGGGTTAGAAATTATAACCGAGATCATCTGTTGCATTTGCATTGTCCTATAAATTATAATCATGTAGACTCACAATGTTGTCTATGCAGGAGGAAACTAACTCGACTCCGTCTTCAGCTAGGCATAAAAGGGGATGCAATTGTAGAAAATCAAGTTGCTTGA

Protein Analysis

594

Amino Acids

65.15

Weight (kDa)

6.7

Isoelectric Point (pI)

61.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCR PF03638 469 - 504 8.3e-11 Tesmin/TSO1-like CXC domain, cysteine-rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019245)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03350 FvH4_6g03350
malus_domestica MD04G1221400.v1.1 MD04G1221500.v1.1 MD12G1237500.v1.1
prunus_persica Prupe.6G340600_v2.0.a1
pyrus_communis pycom04g19580 pycom12g21840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1635, 1677
Acc16I TGCGCA 1 cut(s) 524
AccI GTMKAC 2 cut(s) 901, 1686
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 5 cut(s) 11, 117, 185, 561, 1025
AclWI GGATC 3 cut(s) 638, 653, 857
AcsI RAATTY 4 cut(s) 403, 528, 602, 1204
AcuI CTGAAG 3 cut(s) 35, 351, 1718
AfaI GTAC 4 cut(s) 331, 393, 474, 1231
AfeI AGCGCT 1 cut(s) 1409
AfiI CCNNNNNNNGG 2 cut(s) 1115, 1619
AflII CTTAAG 1 cut(s) 1159
AgeI ACCGGT 1 cut(s) 415
AjnI CCWGG 2 cut(s) 661, 1108
AluBI AGCT 7 cut(s) 152, 383, 815, 912, 977, 1283, 1738
AluI AGCT 7 cut(s) 152, 383, 815, 912, 977, 1283, 1738
Alw26I GTCTC 2 cut(s) 690, 1472
AlwI GGATC 3 cut(s) 638, 653, 857
AlwNI CAGNNNCTG 1 cut(s) 951
Ama87I CYCGRG 1 cut(s) 254
Aor13HI TCCGGA 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 1409
AoxI GGCC 1 cut(s) 968
ApeKI GCWGC 8 cut(s) 229, 557, 912, 974, 1316, 1378, 1410, 1460
ApoI RAATTY 4 cut(s) 403, 528, 602, 1204
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AsiGI ACCGGT 1 cut(s) 415
Asp700I GAANNNNTTC 2 cut(s) 364, 786
AspLEI GCGC 4 cut(s) 525, 951, 1369, 1410
AspS9I GGNCC 2 cut(s) 728, 968
AsuHPI GGTGA 5 cut(s) 19, 121, 237, 586, 971
AsuII TTCGAA 1 cut(s) 482
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 728
BauI CACGAG 1 cut(s) 1257
BbsI GAAGAC 1 cut(s) 1724
BbvI GCAGC 8 cut(s) 241, 569, 899, 986, 1328, 1390, 1397, 1447
BccI CCATC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 1586
BcgI CGANNNNNNTGC 2 cut(s) 1439, 1473
BciT130I CCWGG 2 cut(s) 663, 1110
BciVI GTATCC 1 cut(s) 505
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 2 cut(s) 690, 1472
BfaI CTAG 9 cut(s) 149, 384, 741, 909, 921, 1002, 1146, 1227, 1739
BfoI RGCGCY 2 cut(s) 952, 1411
BfrI CTTAAG 1 cut(s) 1159
BfuI GTATCC 1 cut(s) 505
BmcAI AGTACT 1 cut(s) 1231
Bme1390I CCNGG 2 cut(s) 663, 1110
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 2 cut(s) 728, 968
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 2 cut(s) 663, 1110
BmrI ACTGGG 1 cut(s) 1239
BmsI GCATC 5 cut(s) 167, 362, 544, 988, 1744
BmuI ACTGGG 1 cut(s) 1239
BpiI GAAGAC 1 cut(s) 1724
Bpu10I CCTNAGC 2 cut(s) 1284, 1370
Bpu14I TTCGAA 1 cut(s) 482
Bsa29I ATCGAT 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 1472
BsaWI WCCGGW 4 cut(s) 301, 340, 356, 415
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 1115, 1619
Bse118I RCCGGY 2 cut(s) 106, 415
Bse1I ACTGG 4 cut(s) 653, 846, 1032, 1245
Bse8I GATNNNNATC 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 2 cut(s) 663, 1110
BseCI ATCGAT 1 cut(s) 735
BseGI GGATG 3 cut(s) 353, 687, 1759
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 1115, 1619
BseMII CTCAG 6 cut(s) 390, 524, 941, 1179, 1298, 1341
BseNI ACTGG 4 cut(s) 653, 846, 1032, 1245
BseRI GAGGAG 2 cut(s) 30, 1290
BseXI GCAGC 8 cut(s) 241, 569, 899, 986, 1328, 1390, 1397, 1447
BsgI GTGCAG 2 cut(s) 408, 1335
BshFI GGCC 1 cut(s) 970
BshTI ACCGGT 1 cut(s) 415
BshVI ATCGAT 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 6 cut(s) 107, 302, 341, 357, 416, 818
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 1115, 1619
BsmAI GTCTC 2 cut(s) 690, 1472
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 3 cut(s) 350, 1135, 1505
BsnI GGCC 1 cut(s) 970
Bso31I GGTCTC 1 cut(s) 1472
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 482
Bsp13I TCCGGA 1 cut(s) 356
Bsp1407I TGTACA 1 cut(s) 472
BspACI CCGC 5 cut(s) 11, 117, 185, 561, 1025
BspANI GGCC 1 cut(s) 970
BspCNI CTCAG 6 cut(s) 391, 525, 940, 1180, 1297, 1342
BspDI ATCGAT 1 cut(s) 735
BspEI TCCGGA 1 cut(s) 356
BspLI GGNNCC 1 cut(s) 411
BspPI GGATC 3 cut(s) 638, 653, 857
BspT104I TTCGAA 1 cut(s) 482
BspTI CTTAAG 1 cut(s) 1159
BspTNI GGTCTC 1 cut(s) 1472
BsrFI RCCGGY 2 cut(s) 106, 415
BsrGI TGTACA 1 cut(s) 472
BsrI ACTGG 4 cut(s) 653, 846, 1032, 1245
BssAI RCCGGY 2 cut(s) 106, 415
BssNAI GTATAC 1 cut(s) 902
BssSI CACGAG 1 cut(s) 1257
Bst1107I GTATAC 1 cut(s) 902
Bst2BI CACGAG 1 cut(s) 1257
Bst2UI CCWGG 2 cut(s) 663, 1110
Bst4CI ACNGT 5 cut(s) 213, 284, 1340, 1418, 1475
Bst6I CTCTTC 2 cut(s) 48, 168
BstAFI CTTAAG 1 cut(s) 1159
BstAPI GCANNNNNTGC 1 cut(s) 1410
BstAUI TGTACA 1 cut(s) 472
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 8 cut(s) 318, 565, 917, 947, 972, 1365, 1402, 1406
BstDEI CTNAG 9 cut(s) 221, 399, 533, 798, 927, 1188, 1284, 1350, 1370
BstEII GGTNACC 1 cut(s) 1104
BstF5I GGATG 3 cut(s) 353, 687, 1759
BstH2I RGCGCY 2 cut(s) 952, 1411
BstHHI GCGC 4 cut(s) 525, 951, 1369, 1410
BstMAI GTCTC 2 cut(s) 690, 1472
BstMWI GCNNNNNNNGC 3 cut(s) 1289, 1375, 1410
BstNI CCWGG 2 cut(s) 663, 1110
BstNSI RCATGY 2 cut(s) 567, 1367
BstPI GGTNACC 1 cut(s) 1104
BstSCI CCNGG 2 cut(s) 661, 1108
BstV1I GCAGC 8 cut(s) 241, 569, 899, 986, 1328, 1390, 1397, 1447
BstV2I GAAGAC 1 cut(s) 1724
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BstZ17I GTATAC 1 cut(s) 902
Bsu15I ATCGAT 1 cut(s) 735
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 1 cut(s) 970
BsuTUI ATCGAT 1 cut(s) 735
BtsCI GGATG 3 cut(s) 353, 687, 1759
BtsIMutI CAGTG 1 cut(s) 1252
Cac8I GCNNGC 8 cut(s) 318, 565, 917, 947, 972, 1365, 1402, 1406
CaiI CAGNNNCTG 1 cut(s) 951
CfoI GCGC 4 cut(s) 525, 951, 1369, 1410
Cfr10I RCCGGY 2 cut(s) 106, 415
Cfr13I GGNCC 2 cut(s) 728, 968
ClaI ATCGAT 1 cut(s) 735
Csp6I GTAC 4 cut(s) 330, 392, 473, 1230
CspAI ACCGGT 1 cut(s) 415
CviAII CATG 7 cut(s) 564, 1178, 1269, 1334, 1364, 1491, 1682
CviQI GTAC 4 cut(s) 330, 392, 473, 1230
DdeI CTNAG 9 cut(s) 221, 399, 533, 798, 927, 1188, 1284, 1350, 1370
Eam1104I CTCTTC 2 cut(s) 48, 168
EarI CTCTTC 2 cut(s) 48, 168
Eco31I GGTCTC 1 cut(s) 1472
Eco32I GATATC 1 cut(s) 1224
Eco47I GGWCC 1 cut(s) 728
Eco47III AGCGCT 1 cut(s) 1409
Eco57I CTGAAG 3 cut(s) 35, 351, 1718
Eco88I CYCGRG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 1104
EcoO65I GGTNACC 1 cut(s) 1104
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 2 cut(s) 661, 1108
EcoRV GATATC 1 cut(s) 1224
FaeI CATG 7 cut(s) 567, 1181, 1272, 1337, 1367, 1494, 1685
FalI AAGNNNNNCTT 2 cut(s) 1384, 1416
FaqI GGGAC 1 cut(s) 185
FatI CATG 7 cut(s) 563, 1177, 1268, 1333, 1363, 1490, 1681
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 2 cut(s) 901, 1686
FokI GGATG 3 cut(s) 340, 674, 1766
FspBI CTAG 9 cut(s) 149, 384, 741, 909, 921, 1002, 1146, 1227, 1739
FspI TGCGCA 1 cut(s) 524
GlaI GCGC 4 cut(s) 524, 950, 1368, 1409
HaeII RGCGCY 2 cut(s) 952, 1411
HaeIII GGCC 1 cut(s) 970
HapII CCGG 6 cut(s) 107, 302, 341, 357, 416, 818
HhaI GCGC 4 cut(s) 525, 951, 1369, 1410
Hin1II CATG 7 cut(s) 567, 1181, 1272, 1337, 1367, 1494, 1685
Hin6I GCGC 4 cut(s) 523, 949, 1367, 1408
HinP1I GCGC 4 cut(s) 523, 949, 1367, 1408
HpaII CCGG 6 cut(s) 107, 302, 341, 357, 416, 818
HphI GGTGA 5 cut(s) 19, 121, 237, 586, 971
Hpy166II GTNNAC 3 cut(s) 112, 902, 1687
Hpy188I TCNGA 6 cut(s) 24, 241, 370, 534, 589, 1189
Hpy188III TCNNGA 8 cut(s) 265, 357, 505, 831, 1259, 1328, 1349, 1496
Hpy8I GTNNAC 3 cut(s) 112, 902, 1687
HpyAV CCTTC 6 cut(s) 177, 219, 271, 483, 776, 959
HpyCH4III ACNGT 5 cut(s) 213, 284, 1340, 1418, 1475
HpyF10VI GCNNNNNNNGC 3 cut(s) 1289, 1375, 1410
HpyF3I CTNAG 9 cut(s) 221, 399, 533, 798, 927, 1188, 1284, 1350, 1370
Hsp92II CATG 7 cut(s) 567, 1181, 1272, 1337, 1367, 1494, 1685
HspAI GCGC 4 cut(s) 523, 949, 1367, 1408
Kpn2I TCCGGA 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 592
LmnI GCTCC 2 cut(s) 820, 1585
Lsp1109I GCAGC 8 cut(s) 241, 569, 899, 986, 1328, 1390, 1397, 1447
LweI GCATC 5 cut(s) 167, 362, 544, 988, 1744
MaeI CTAG 9 cut(s) 149, 384, 741, 909, 921, 1002, 1146, 1227, 1739
MaeIII GTNAC 3 cut(s) 1104, 1154, 1588
MboII GAAGA 9 cut(s) 28, 35, 155, 357, 559, 758, 1089, 1366, 1724
MfeI CAATTG 2 cut(s) 99, 1758
MflI RGATCY 1 cut(s) 658
MlyI GAGTC 5 cut(s) 266, 1201, 1234, 1682, 1719
MmeI TCCRAC 1 cut(s) 1366
MroI TCCGGA 1 cut(s) 356
MroXI GAANNNNTTC 2 cut(s) 364, 786
MseI TTAA 5 cut(s) 599, 621, 851, 858, 1160
MslI CAYNNNNRTG 2 cut(s) 465, 1128
MspA1I CMGCKG 1 cut(s) 13
MspCI CTTAAG 1 cut(s) 1159
MspI CCGG 6 cut(s) 107, 302, 341, 357, 416, 818
MspR9I CCNGG 2 cut(s) 663, 1110
MunI CAATTG 2 cut(s) 99, 1758
Mva1269I GAATGC 3 cut(s) 350, 1135, 1505
MvaI CCWGG 2 cut(s) 663, 1110
MwoI GCNNNNNNNGC 3 cut(s) 1289, 1375, 1410
NlaIII CATG 7 cut(s) 567, 1181, 1272, 1337, 1367, 1494, 1685
NlaIV GGNNCC 1 cut(s) 411
NsbI TGCGCA 1 cut(s) 524
NspI RCATGY 2 cut(s) 567, 1367
NspV TTCGAA 1 cut(s) 482
PaeI GCATGC 2 cut(s) 567, 1367
PcsI WCGNNNNNNNCGW 1 cut(s) 1050
PctI GAATGC 3 cut(s) 350, 1135, 1505
PdmI GAANNNNTTC 2 cut(s) 364, 786
PfeI GAWTC 9 cut(s) 19, 62, 190, 242, 719, 737, 1265, 1540, 1556
PflFI GACNNNGTC 1 cut(s) 1728
PinAI ACCGGT 1 cut(s) 415
PleI GAGTC 5 cut(s) 265, 1200, 1234, 1682, 1719
PpsI GAGTC 5 cut(s) 265, 1200, 1234, 1682, 1719
PsiI TTATAA 2 cut(s) 1635, 1677
Psp6I CCWGG 2 cut(s) 661, 1108
PspEI GGTNACC 1 cut(s) 1104
PspGI CCWGG 2 cut(s) 661, 1108
PspN4I GGNNCC 1 cut(s) 411
PspPI GGNCC 2 cut(s) 728, 968
PstNI CAGNNNCTG 1 cut(s) 951
PsuI RGATCY 1 cut(s) 658
PsyI GACNNNGTC 1 cut(s) 1728
RsaI GTAC 4 cut(s) 331, 393, 474, 1231
RsaNI GTAC 4 cut(s) 330, 392, 473, 1230
RseI CAYNNNNRTG 2 cut(s) 465, 1128
SaqAI TTAA 5 cut(s) 599, 621, 851, 858, 1160
Sau96I GGNCC 2 cut(s) 728, 968
ScaI AGTACT 1 cut(s) 1231
SchI GAGTC 5 cut(s) 266, 1201, 1234, 1682, 1719
ScrFI CCNGG 2 cut(s) 663, 1110
SfaNI GCATC 5 cut(s) 167, 362, 544, 988, 1744
SfuI TTCGAA 1 cut(s) 482
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 2 cut(s) 465, 1128
SmlI CTYRAG 1 cut(s) 1159
SmoI CTYRAG 1 cut(s) 1159
SphI GCATGC 2 cut(s) 567, 1367
SsiI CCGC 5 cut(s) 11, 117, 185, 561, 1025
SspI AATATT 1 cut(s) 1528
SspMI CTAG 9 cut(s) 149, 384, 741, 909, 921, 1002, 1146, 1227, 1739
StyD4I CCNGG 2 cut(s) 661, 1108
TaaI ACNGT 5 cut(s) 213, 284, 1340, 1418, 1475
TaqI TCGA 6 cut(s) 482, 504, 735, 1053, 1195, 1723
TatI WGTACW 2 cut(s) 472, 1229
TauI GCSGC 2 cut(s) 187, 563
TfiI GAWTC 9 cut(s) 19, 62, 190, 242, 719, 737, 1265, 1540, 1556
Tru1I TTAA 5 cut(s) 599, 621, 851, 858, 1160
Tru9I TTAA 5 cut(s) 599, 621, 851, 858, 1160
TscAI CASTG 1 cut(s) 1252
TseI GCWGC 8 cut(s) 229, 557, 912, 974, 1316, 1378, 1410, 1460
TspDTI ATGAA 3 cut(s) 1083, 1278, 1479
TspGWI ACGGA 2 cut(s) 187, 1718
TspRI CASTG 1 cut(s) 1252
Tth111I GACNNNGTC 1 cut(s) 1728
Vha464I CTTAAG 1 cut(s) 1159
VpaK11BI GGWCC 1 cut(s) 728
XapI RAATTY 4 cut(s) 403, 528, 602, 1204
XceI RCATGY 2 cut(s) 567, 1367
XmiI GTMKAC 2 cut(s) 901, 1686
XmnI GAANNNNTTC 2 cut(s) 364, 786
XspI CTAG 9 cut(s) 149, 384, 741, 909, 921, 1002, 1146, 1227, 1739
ZrmI AGTACT 1 cut(s) 1231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.