Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0019245

Physical Location & Seq

Genomic Coordinates Forward (+)

30277659 .. 30280393

Gene Structure 2735 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1221500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGTTAATGATCCAAGTGGTATTGTGGATGAAAAGTCGCCGAAGTGTATACATTCTAGCTGCAAGTTCTCAGTCATACGCAATACGCAGACGCTGTTTGGATTTTGAAAGGCCAGCAACGTACAAAAGGAAATCAATAGATGCCAGTGGCGATTCTTCAGCCTCAGTACAGTCCAGTTGTGAAGTCGCCTCAGTCGAAAAGCAGTTGGTTCAAACTATAAAAGGTTGTGATTATTCATCTTCTCGGTTACCTGGTATTGGTTTGCACTTGAATGCTCTTGCAACTACTATTGAAAGTAATTTAAGTACGGTTGTCAAGCACGAGGTTCACGCTTCTGAGAGTCAAGCGATTAATTTGCCAAACTCCAAGATATCTAGTACTTCTTTGACTCCCAGTGAGGTTTCTCGTGATGAATCATGTAAAGATAAAGCTCAGGTTGCGGTAACTTCACCCCAAATATGTGCACCAGGTGGTCAAGAACCTGACCATAGTAGTCCTGAGAAGAAAAGGCTTAAGTTGCAGCCTGCTGGAGAAAGCTTGGCTTGCAAACGCTGCAATTGTAAGAGATCAAAATGCTTGAAACTTTATTGCGACTGTTTTGCTGCTGGTCTCTACTGTATTGAGCCTTGTTCATGTCAAGAATGCTTCAACAAGCCTATTTATGAGAACATTGTTTTAGAGAATCGCAAACTGATTGAATCCCGCAACCCACTTGCATTTGCTCCCAAAGTGATTGCAAAAGCCGTTGCTATTCCACAGTATGCGGAGGAAACTAACTCGACTCCGTCTTCAGCTAGGCATAAAAGGGGATGCAATTGTAGAAAATCAAGTTGCTTGAAAAAGTACTGTGAATGCTTTCAGGGCGGTGCTGGATGCGGCATCCTCTGCAGATGTGTAGGGTGTAAAAACACTTTTGGTCGGAAAGATGAAGCTGAACAAGCTGAAGTTGGAGGAGATGAATCAGTAGATCCGAGAAAGGATGCAGTGAATGTCAGTTTACCAACAGTGAGGGATCAAGATCTCCTAATGGCACCTCCGGGGATTTGCAGACCTCCTCCAGGCCAACTGACATCTTCTTCTAAGGGGAGACCGAAAATATTTTCTCTGCATTCTGTTCTGTCATCTTCTCAGCATGAAAAGCATCTTCAGGCAATTCCAGAAGACGTAACTCTTGAGACGTTGGCTAGCAGTTGCTCACAACCTAGCGGTTTGAAGTCAACCTCTCCAAACTCAAAGAGGGTATCGCCTCCTCACAGCAGCGGTGAGTTTGGATCAGCTTGGAGGGGTGGCAGGAAGTTAGTTCTGAGATCCATTCCACCATTCCCAACTCTGGAATCACCACGAAAGCAGTCACTTCCGCCACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

TCR

Protein Analysis

456

Amino Acids

49.4

Weight (kDa)

9.05

Isoelectric Point (pI)

71.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TCR	PF03638	183 - 218	5.9e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain
TCR	PF03638	268 - 304	4.9e-12	Tesmin/TSO1-like CXC domain, cysteine-rich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019245)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g03350 FvH4_6g03350
malus_domestica	MD04G1221400.v1.1 MD04G1221500.v1.1 MD12G1237500.v1.1
prunus_persica	Prupe.6G340600_v2.0.a1
pyrus_communis	pycom04g19580 pycom12g21840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1030
AccI	GTMKAC	1 cut(s)	48
AciI	CCGC	8 cut(s)	440, 703, 764, 864, 876, 1206, 1260, 1358
AclWI	GGATC	5 cut(s)	4, 962, 1020, 1279, 1302
AcuI	CTGAAG	4 cut(s)	141, 774, 963, 1130
AdeI	CACNNNGTG	1 cut(s)	470
AfaI	GTAC	5 cut(s)	122, 168, 307, 379, 845
AfiI	CCNNNNNNNGG	3 cut(s)	257, 1058, 1330
AflII	CTTAAG	1 cut(s)	512
AgsI	TTSAA	9 cut(s)	107, 212, 271, 293, 580, 649, 698, 838, 1213
AjnI	CCWGG	3 cut(s)	250, 466, 1057
AluBI	AGCT	7 cut(s)	59, 431, 537, 794, 932, 941, 1277
AluI	AGCT	7 cut(s)	59, 431, 537, 794, 932, 941, 1277
Alw21I	GWGCWC	1 cut(s)	466
Alw26I	GTCTC	3 cut(s)	614, 1081, 1169
Alw44I	GTGCAC	1 cut(s)	462
AlwI	GGATC	5 cut(s)	4, 962, 1020, 1279, 1302
AlwNI	CAGNNNCTG	1 cut(s)	93
AoxI	GGCC	2 cut(s)	110, 1060
ApaLI	GTGCAC	1 cut(s)	462
ApeKI	GCWGC	5 cut(s)	59, 520, 552, 602, 1257
AseI	ATTAAT	1 cut(s)	351
Asp700I	GAANNNNTTC	1 cut(s)	855
AsuC2I	CCSGG	1 cut(s)	1038
AsuHPI	GGTGA	3 cut(s)	441, 1274, 1329
AsuNHI	GCTAGC	1 cut(s)	1184
BaeGI	GKGCMC	1 cut(s)	466
BanI	GGYRCC	1 cut(s)	1030
BauI	CACGAG	2 cut(s)	320, 405
BbsI	GAAGAC	2 cut(s)	780, 1167
Bbv12I	GWGCWC	1 cut(s)	466
BbvI	GCAGC	5 cut(s)	46, 532, 539, 589, 1269
BceAI	ACGGC	1 cut(s)	728
BcgI	CGANNNNNNTGC	4 cut(s)	337, 371, 581, 615
BciT130I	CCWGG	3 cut(s)	252, 468, 1059
BcnI	CCSGG	1 cut(s)	1038
BcoDI	GTCTC	3 cut(s)	614, 1081, 1169
BfaI	CTAG	5 cut(s)	56, 375, 795, 1185, 1203
BfmI	CTRYAG	1 cut(s)	886
BfrI	CTTAAG	1 cut(s)	512
BglII	AGATCT	1 cut(s)	1018
BisI	GCNGC	6 cut(s)	60, 521, 553, 603, 877, 1258
BlsI	GCNGC	6 cut(s)	61, 522, 554, 604, 878, 1259
BmcAI	AGTACT	2 cut(s)	379, 845
Bme1390I	CCNGG	4 cut(s)	252, 468, 1038, 1059
BmiI	GGNNCC	1 cut(s)	1032
BmrFI	CCNGG	4 cut(s)	252, 468, 1038, 1059
BmrI	ACTGGG	1 cut(s)	387
BmsI	GCATC	6 cut(s)	130, 800, 863, 888, 970, 1150
BmtI	GCTAGC	1 cut(s)	1188
BmuI	ACTGGG	1 cut(s)	387
BpiI	GAAGAC	2 cut(s)	780, 1167
BpmI	CTGGAG	2 cut(s)	549, 1041
Bpu10I	CCTNAGC	1 cut(s)	432
BpuEI	CTTGAG	1 cut(s)	1193
BpuMI	CCSGG	1 cut(s)	1038
BsaBI	GATNNNNATC	1 cut(s)	1017
BsaI	GGTCTC	2 cut(s)	614, 1081
BsaJI	CCNNGG	1 cut(s)	1037
Bsc4I	CCNNNNNNNGG	3 cut(s)	257, 1058, 1330
Bse1I	ACTGG	3 cut(s)	144, 174, 393
Bse8I	GATNNNNATC	1 cut(s)	1017
BseBI	CCWGG	3 cut(s)	252, 468, 1059
BseDI	CCNNGG	1 cut(s)	1037
BseGI	GGATG	5 cut(s)	33, 815, 878, 879, 985
BseJI	GATNNNNATC	1 cut(s)	1017
BseLI	CCNNNNNNNGG	3 cut(s)	257, 1058, 1330
BseMII	CTCAG	8 cut(s)	83, 177, 204, 327, 446, 489, 1142, 1295
BseNI	ACTGG	3 cut(s)	144, 174, 393
BseRI	GAGGAG	3 cut(s)	966, 1044, 1239
BseSI	GKGCMC	1 cut(s)	466
BseXI	GCAGC	5 cut(s)	46, 532, 539, 589, 1269
BshFI	GGCC	2 cut(s)	112, 1062
BshNI	GGYRCC	1 cut(s)	1030
BsiHKAI	GWGCWC	1 cut(s)	466
BsiSI	CCGG	1 cut(s)	1037
BslI	CCNNNNNNNGG	3 cut(s)	257, 1058, 1330
BsmAI	GTCTC	3 cut(s)	614, 1081, 1169
BsmBI	CGTCTC	1 cut(s)	1169
BsmI	GAATGC	4 cut(s)	277, 647, 857, 1108
BsnI	GGCC	2 cut(s)	112, 1062
Bso31I	GGTCTC	2 cut(s)	614, 1081
Bsp1286I	GDGCHC	1 cut(s)	466
Bsp143I	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
BspACI	CCGC	8 cut(s)	440, 703, 764, 864, 876, 1206, 1260, 1358
BspANI	GGCC	2 cut(s)	112, 1062
BspCNI	CTCAG	8 cut(s)	82, 176, 203, 328, 445, 490, 1141, 1296
BspLI	GGNNCC	1 cut(s)	1032
BspMAI	CTGCAG	1 cut(s)	890
BspOI	GCTAGC	1 cut(s)	1188
BspPI	GGATC	5 cut(s)	4, 962, 1020, 1279, 1302
BspT107I	GGYRCC	1 cut(s)	1030
BspTI	CTTAAG	1 cut(s)	512
BspTNI	GGTCTC	2 cut(s)	614, 1081
BsrI	ACTGG	3 cut(s)	144, 174, 393
BssECI	CCNNGG	1 cut(s)	1037
BssMI	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
BssNAI	GTATAC	1 cut(s)	49
BssSI	CACGAG	2 cut(s)	320, 405
Bst1107I	GTATAC	1 cut(s)	49
Bst2BI	CACGAG	2 cut(s)	320, 405
Bst2UI	CCWGG	3 cut(s)	252, 468, 1059
Bst4CI	ACNGT	7 cut(s)	171, 310, 596, 617, 759, 848, 1006
BstAFI	CTTAAG	1 cut(s)	512
BstAPI	GCANNNNNTGC	2 cut(s)	552, 885
BstC8I	GCNNGC	4 cut(s)	114, 525, 544, 1186
BstDEI	CTNAG	9 cut(s)	69, 163, 190, 336, 432, 498, 1080, 1128, 1304
BstEII	GGTNACC	1 cut(s)	246
BstENI	CCTNNNNNAGG	1 cut(s)	1056
BstF5I	GGATG	5 cut(s)	33, 815, 878, 879, 985
BstKTI	GATC	7 cut(s)	12, 569, 970, 1015, 1021, 1274, 1310
BstMAI	GTCTC	3 cut(s)	614, 1081, 1169
BstMBI	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
BstMWI	GCNNNNNNNGC	8 cut(s)	437, 517, 543, 552, 861, 885, 938, 1138
BstNI	CCWGG	3 cut(s)	252, 468, 1059
BstPI	GGTNACC	1 cut(s)	246
BstSCI	CCNGG	4 cut(s)	250, 466, 1036, 1057
BstSFI	CTRYAG	1 cut(s)	886
BstSLI	GKGCMC	1 cut(s)	466
BstV1I	GCAGC	5 cut(s)	46, 532, 539, 589, 1269
BstV2I	GAAGAC	2 cut(s)	780, 1167
BstX2I	RGATCY	3 cut(s)	967, 1018, 1307
BstYI	RGATCY	3 cut(s)	967, 1018, 1307
BstZ17I	GTATAC	1 cut(s)	49
BsuRI	GGCC	2 cut(s)	112, 1062
BtsCI	GGATG	5 cut(s)	33, 815, 878, 879, 985
BtsI	GCAGTG	1 cut(s)	990
BtsIMutI	CAGTG	4 cut(s)	151, 400, 990, 1011
Cac8I	GCNNGC	4 cut(s)	114, 525, 544, 1186
CaiI	CAGNNNCTG	1 cut(s)	93
CseI	GACGC	1 cut(s)	99
CsiI	ACCWGGT	2 cut(s)	250, 466
Csp6I	GTAC	5 cut(s)	121, 167, 306, 378, 844
CviAII	CATG	3 cut(s)	417, 633, 1133
CviQI	GTAC	5 cut(s)	121, 167, 306, 378, 844
DdeI	CTNAG	9 cut(s)	69, 163, 190, 336, 432, 498, 1080, 1128, 1304
DpnI	GATC	7 cut(s)	11, 568, 969, 1014, 1020, 1273, 1309
DpnII	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
DraIII	CACNNNGTG	1 cut(s)	470
EciI	GGCGGA	1 cut(s)	1347
Eco31I	GGTCTC	2 cut(s)	614, 1081
Eco32I	GATATC	1 cut(s)	372
Eco57I	CTGAAG	4 cut(s)	141, 774, 963, 1130
Eco91I	GGTNACC	1 cut(s)	246
EcoNI	CCTNNNNNAGG	1 cut(s)	1056
EcoO65I	GGTNACC	1 cut(s)	246
EcoRII	CCWGG	3 cut(s)	250, 466, 1057
EcoRV	GATATC	1 cut(s)	372
Esp3I	CGTCTC	1 cut(s)	1169
FaeI	CATG	3 cut(s)	420, 636, 1136
FalI	AAGNNNNNCTT	2 cut(s)	526, 558
FatI	CATG	3 cut(s)	416, 632, 1132
FauI	CCCGC	1 cut(s)	710
FblI	GTMKAC	1 cut(s)	48
Fnu4HI	GCNGC	6 cut(s)	60, 521, 553, 603, 877, 1258
FokI	GGATG	5 cut(s)	40, 822, 866, 885, 992
Fsp4HI	GCNGC	6 cut(s)	60, 521, 553, 603, 877, 1258
FspBI	CTAG	5 cut(s)	56, 375, 795, 1185, 1203
GluI	GCNGC	6 cut(s)	60, 521, 553, 603, 877, 1258
GsuI	CTGGAG	2 cut(s)	549, 1041
HaeIII	GGCC	2 cut(s)	112, 1062
HapII	CCGG	1 cut(s)	1037
HgaI	GACGC	1 cut(s)	99
Hin1II	CATG	3 cut(s)	420, 636, 1136
HincII	GTYRAC	1 cut(s)	1218
HindII	GTYRAC	1 cut(s)	1218
HindIII	AAGCTT	1 cut(s)	535
HinfI	GANTC	9 cut(s)	152, 340, 388, 413, 682, 698, 781, 959, 1334
HpaII	CCGG	1 cut(s)	1037
HphI	GGTGA	3 cut(s)	441, 1274, 1329
Hpy166II	GTNNAC	5 cut(s)	49, 328, 464, 998, 1218
Hpy188I	TCNGA	4 cut(s)	337, 921, 972, 1305
Hpy188III	TCNNGA	8 cut(s)	407, 476, 497, 638, 1016, 1157, 1172, 1331
Hpy8I	GTNNAC	5 cut(s)	49, 328, 464, 998, 1218
HpyCH4III	ACNGT	7 cut(s)	171, 310, 596, 617, 759, 848, 1006
HpyCH4IV	ACGT	3 cut(s)	119, 1164, 1178
HpyF10VI	GCNNNNNNNGC	8 cut(s)	437, 517, 543, 552, 861, 885, 938, 1138
HpyF3I	CTNAG	9 cut(s)	69, 163, 190, 336, 432, 498, 1080, 1128, 1304
HpySE526I	ACGT	3 cut(s)	119, 1164, 1178
Hsp92II	CATG	3 cut(s)	420, 636, 1136
Kzo9I	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
LmnI	GCTCC	1 cut(s)	727
Lsp1109I	GCAGC	5 cut(s)	46, 532, 539, 589, 1269
LweI	GCATC	6 cut(s)	130, 800, 863, 888, 970, 1150
MabI	ACCWGGT	2 cut(s)	250, 466
MaeI	CTAG	5 cut(s)	56, 375, 795, 1185, 1203
MaeII	ACGT	3 cut(s)	119, 1164, 1178
MaeIII	GTNAC	4 cut(s)	246, 442, 1165, 1350
MalI	GATC	7 cut(s)	11, 568, 969, 1014, 1020, 1273, 1309
MboI	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
MboII	GAAGA	9 cut(s)	147, 231, 514, 780, 1065, 1068, 1116, 1136, 1172
MfeI	CAATTG	2 cut(s)	556, 814
MflI	RGATCY	3 cut(s)	967, 1018, 1307
MhlI	GDGCHC	1 cut(s)	466
MluCI	AATT	5 cut(s)	298, 352, 556, 814, 1152
MlyI	GAGTC	3 cut(s)	349, 382, 775
MmeI	TCCRAC	2 cut(s)	899, 928
MroXI	GAANNNNTTC	1 cut(s)	855
MseI	TTAA	4 cut(s)	5, 302, 351, 513
MslI	CAYNNNNRTG	1 cut(s)	270
MspA1I	CMGCKG	1 cut(s)	1260
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	1 cut(s)	1037
MspR9I	CCNGG	4 cut(s)	252, 468, 1038, 1059
MunI	CAATTG	2 cut(s)	556, 814
Mva1269I	GAATGC	4 cut(s)	277, 647, 857, 1108
MvaI	CCWGG	3 cut(s)	252, 468, 1059
MwoI	GCNNNNNNNGC	8 cut(s)	437, 517, 543, 552, 861, 885, 938, 1138
NciI	CCSGG	1 cut(s)	1038
NdeII	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
NheI	GCTAGC	1 cut(s)	1184
NlaIII	CATG	3 cut(s)	420, 636, 1136
NlaIV	GGNNCC	1 cut(s)	1032
NmuCI	GTSAC	1 cut(s)	1350
PcsI	WCGNNNNNNNCGW	1 cut(s)	192
PctI	GAATGC	4 cut(s)	277, 647, 857, 1108
PdmI	GAANNNNTTC	1 cut(s)	855
PfeI	GAWTC	6 cut(s)	152, 413, 682, 698, 959, 1334
PflFI	GACNNNGTC	1 cut(s)	784
PkrI	GCNGC	6 cut(s)	61, 522, 554, 604, 878, 1259
PleI	GAGTC	3 cut(s)	348, 382, 775
PpsI	GAGTC	3 cut(s)	348, 382, 775
PshBI	ATTAAT	1 cut(s)	351
Psp6I	CCWGG	3 cut(s)	250, 466, 1057
PspEI	GGTNACC	1 cut(s)	246
PspGI	CCWGG	3 cut(s)	250, 466, 1057
PspN4I	GGNNCC	1 cut(s)	1032
PstI	CTGCAG	1 cut(s)	890
PstNI	CAGNNNCTG	1 cut(s)	93
PsuI	RGATCY	3 cut(s)	967, 1018, 1307
PsyI	GACNNNGTC	1 cut(s)	784
RsaI	GTAC	5 cut(s)	122, 168, 307, 379, 845
RsaNI	GTAC	5 cut(s)	121, 167, 306, 378, 844
RseI	CAYNNNNRTG	1 cut(s)	270
SaqAI	TTAA	4 cut(s)	5, 302, 351, 513
SatI	GCNGC	6 cut(s)	60, 521, 553, 603, 877, 1258
Sau3AI	GATC	7 cut(s)	9, 566, 967, 1012, 1018, 1271, 1307
ScaI	AGTACT	2 cut(s)	379, 845
SchI	GAGTC	3 cut(s)	349, 382, 775
ScrFI	CCNGG	4 cut(s)	252, 468, 1038, 1059
SduI	GDGCHC	1 cut(s)	466
SexAI	ACCWGGT	2 cut(s)	250, 466
SfaNI	GCATC	6 cut(s)	130, 800, 863, 888, 970, 1150
SfcI	CTRYAG	1 cut(s)	886
SmiMI	CAYNNNNRTG	1 cut(s)	270
SmlI	CTYRAG	2 cut(s)	512, 1172
SmoI	CTYRAG	2 cut(s)	512, 1172
Sse9I	AATT	5 cut(s)	298, 352, 556, 814, 1152
SsiI	CCGC	8 cut(s)	440, 703, 764, 864, 876, 1206, 1260, 1358
SspI	AATATT	1 cut(s)	1098
SspMI	CTAG	5 cut(s)	56, 375, 795, 1185, 1203
StyD4I	CCNGG	4 cut(s)	250, 466, 1036, 1057
TaaI	ACNGT	7 cut(s)	171, 310, 596, 617, 759, 848, 1006
TaiI	ACGT	3 cut(s)	122, 1167, 1181
TaqI	TCGA	2 cut(s)	195, 779
TaqII	GACCGA	1 cut(s)	1105
TasI	AATT	5 cut(s)	298, 352, 556, 814, 1152
TatI	WGTACW	3 cut(s)	166, 377, 843
TauI	GCSGC	1 cut(s)	879
TfiI	GAWTC	6 cut(s)	152, 413, 682, 698, 959, 1334
Tru1I	TTAA	4 cut(s)	5, 302, 351, 513
Tru9I	TTAA	4 cut(s)	5, 302, 351, 513
TscAI	CASTG	4 cut(s)	151, 400, 990, 1011
TseFI	GTSAC	1 cut(s)	1350
TseI	GCWGC	5 cut(s)	59, 520, 552, 602, 1257
Tsp45I	GTSAC	1 cut(s)	1350
TspDTI	ATGAA	7 cut(s)	44, 225, 426, 621, 942, 972, 1149
TspGWI	ACGGA	1 cut(s)	774
TspRI	CASTG	4 cut(s)	151, 400, 990, 1011
Tth111I	GACNNNGTC	1 cut(s)	784
Vha464I	CTTAAG	1 cut(s)	512
VneI	GTGCAC	1 cut(s)	462
VspI	ATTAAT	1 cut(s)	351
XagI	CCTNNNNNAGG	1 cut(s)	1056
XmiI	GTMKAC	1 cut(s)	48
XmnI	GAANNNNTTC	1 cut(s)	855
XspI	CTAG	5 cut(s)	56, 375, 795, 1185, 1203
ZrmI	AGTACT	2 cut(s)	379, 845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.