MD04G1245800.v1.1

General transcription factor IIH subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016634
Physical Location & Seq
Reverse (-)
32121885 .. 32124018
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1245800.v1.1.491

Sequence Viewer

Length: 213 bp
ATGGTGAACGCTGTTAAAGGACTGTTCATCTCTTGTGACATACCTATGGCGCAATTCATCATCAATTACGACAATTCACTGCCAGCTTCACAGAGGTTCATAATACATGTCTTGGATAGCACTCACCTTTTTGTGCAGCCCCATGCTGCCGAGATGATAAGAAGCGCCATTGCAGAATTTAGAGACCAGAATTCATATGAGAAGCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko03420 map03022 map03420
Pfam Domains
Protein Families
Tfb5

Protein Analysis

71

Amino Acids

7.95

Weight (kDa)

5.78

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tfb5 PF06331 1 - 68 5.7e-27 Transcription factor TFIIH complex subunit Tfb5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016634)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12400 AT1G12400
malus_domestica MD04G1245800.v1.1 MD12G1192500.v1.1
prunus_persica Prupe.5G081100_v2.0.a1
pyrus_communis pycom04g21670 pycom06g06390
rosa_chinensis RchiOBHm_Chr7g0201071
rosa_multiflora Rmu_sc0010648.1_g000001
rosa_roxburghii Rroxscaffold_3G00255540
rosa_samantha Rh7AG187700 Rh7BG191200 Rh7DG193400
rosa_wichuraiana Rw7G016480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 176, 190
AflIII ACRYGT 1 cut(s) 106
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 136, 146
ApoI RAATTY 2 cut(s) 176, 190
AspLEI GCGC 2 cut(s) 52, 167
AsuHPI GGTGA 2 cut(s) 16, 116
BbvI GCAGC 2 cut(s) 133, 148
BcoDI GTCTC 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 2 cut(s) 137, 147
BlsI GCNGC 2 cut(s) 138, 148
BsaI GGTCTC 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 133, 148
BsgI GTGCAG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 177
Bso31I GGTCTC 1 cut(s) 177
BspTNI GGTCTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 52, 167
BstMAI GTCTC 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 133, 148
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 2 cut(s) 52, 167
CviAII CATG 2 cut(s) 107, 143
CviJI RGCY 3 cut(s) 86, 139, 205
CviKI_1 RGCY 3 cut(s) 86, 139, 205
Eco31I GGTCTC 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 190
FaeI CATG 2 cut(s) 110, 146
FaiI YATR 7 cut(s) 41, 47, 101, 108, 144, 196, 198
FatI CATG 2 cut(s) 106, 142
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 137, 147
Fsp4HI GCNGC 2 cut(s) 137, 147
GlaI GCGC 2 cut(s) 51, 166
GluI GCNGC 2 cut(s) 137, 147
HaeII RGCGCY 1 cut(s) 168
HhaI GCGC 2 cut(s) 52, 167
Hin1II CATG 2 cut(s) 110, 146
Hin6I GCGC 2 cut(s) 50, 165
HinP1I GCGC 2 cut(s) 50, 165
HphI GGTGA 2 cut(s) 16, 116
Hpy166II GTNNAC 1 cut(s) 7
Hpy8I GTNNAC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4V TGCA 2 cut(s) 136, 173
Hsp92II CATG 2 cut(s) 110, 146
HspAI GCGC 2 cut(s) 50, 165
LpnPI CCDG 2 cut(s) 96, 200
Lsp1109I GCAGC 2 cut(s) 133, 148
MaeIII GTNAC 1 cut(s) 35
MluCI AATT 5 cut(s) 53, 64, 73, 176, 190
MnlI CCTC 1 cut(s) 87
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 44
NdeI CATATG 1 cut(s) 196
NlaIII CATG 2 cut(s) 110, 146
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 110
PciI ACATGT 1 cut(s) 106
PkrI GCNGC 2 cut(s) 138, 148
PscI ACATGT 1 cut(s) 106
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 2 cut(s) 137, 147
SetI ASST 4 cut(s) 46, 88, 98, 129
SgeI CNNG 7 cut(s) 45, 95, 119, 124, 155, 163, 199
SmiMI CAYNNNNRTG 1 cut(s) 44
Sse9I AATT 5 cut(s) 53, 64, 73, 176, 190
TaaI ACNGT 1 cut(s) 24
TasI AATT 5 cut(s) 53, 64, 73, 176, 190
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 136, 146
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 4 cut(s) 16, 46, 88, 183
TspRI CASTG 1 cut(s) 84
XapI RAATTY 2 cut(s) 176, 190
XceI RCATGY 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.