Rw7G016480

General transcription factor IIH subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016634
Physical Location & Seq
Reverse (-)
16833556 .. 16835943
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016480.1

Sequence Viewer

Length: 213 bp
ATGGTGAATGCTACTAAAGGATTGTTTATCTCTTGTGACATTCCTATGGCGCAATTCATCATCAATTACAACAATTCACTGCCTCCATCACAAAAGTTCATAATACACGTCTTGGATGGCACTCACTTGTTTGTGCAACAGCATGCAGCTGAGATGATACGAAGTGCCATTTCAGAATTTAGAGACCAGAATTCTTATGAGAAGCCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko03420 map03022 map03420
Pfam Domains
Protein Families
Tfb5

Protein Analysis

70

Amino Acids

7.94

Weight (kDa)

6.23

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tfb5 PF06331 1 - 68 2.4e-26 Transcription factor TFIIH complex subunit Tfb5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016634)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12400 AT1G12400
malus_domestica MD04G1245800.v1.1 MD12G1192500.v1.1
prunus_persica Prupe.5G081100_v2.0.a1
pyrus_communis pycom04g21670 pycom06g06390
rosa_chinensis RchiOBHm_Chr7g0201071
rosa_multiflora Rmu_sc0010648.1_g000001
rosa_roxburghii Rroxscaffold_3G00255540
rosa_samantha Rh7AG187700 Rh7BG191200 Rh7DG193400
rosa_wichuraiana Rw7G016480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 176, 190
AflIII ACRYGT 1 cut(s) 106
AjiI CACGTC 1 cut(s) 109
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
Alw26I GTCTC 1 cut(s) 177
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 2 cut(s) 176, 190
AspLEI GCGC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 16
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 94, 110
BcoDI GTCTC 1 cut(s) 177
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 109
BsaI GGTCTC 1 cut(s) 177
BseGI GGATG 1 cut(s) 121
BseMII CTCAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 177
BsmI GAATGC 1 cut(s) 13
Bso31I GGTCTC 1 cut(s) 177
BspCNI CTCAG 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 177
BstC8I GCNNGC 2 cut(s) 144, 207
BstDEI CTNAG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 121
BstHHI GCGC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 158
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 2 cut(s) 144, 207
CfoI GCGC 1 cut(s) 52
CviAII CATG 1 cut(s) 143
CviJI RGCY 2 cut(s) 149, 205
CviKI_1 RGCY 2 cut(s) 149, 205
DdeI CTNAG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 190
FaeI CATG 1 cut(s) 146
FaiI YATR 4 cut(s) 47, 101, 144, 198
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 147
FspEI CC 9 cut(s) 3, 32, 57, 96, 98, 99, 102, 181, 200
GlaI GCGC 1 cut(s) 51
GluI GCNGC 1 cut(s) 147
HhaI GCGC 1 cut(s) 52
Hin1II CATG 1 cut(s) 146
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 136, 146
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 1 cut(s) 146
HspAI GCGC 1 cut(s) 50
LpnPI CCDG 1 cut(s) 200
Lsp1109I GCAGC 1 cut(s) 158
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 35
MluCI AATT 5 cut(s) 53, 64, 73, 176, 190
MnlI CCTC 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 146
PaeI GCATGC 1 cut(s) 146
PctI GAATGC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 44
SatI GCNGC 1 cut(s) 147
SetI ASST 2 cut(s) 111, 151
SgeI CNNG 6 cut(s) 45, 119, 124, 139, 155, 199
SmiMI CAYNNNNRTG 1 cut(s) 44
SphI GCATGC 1 cut(s) 146
Sse9I AATT 5 cut(s) 53, 64, 73, 176, 190
TaiI ACGT 1 cut(s) 111
TasI AATT 5 cut(s) 53, 64, 73, 176, 190
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 2 cut(s) 46, 88
TspRI CASTG 1 cut(s) 84
XapI RAATTY 2 cut(s) 176, 190
XceI RCATGY 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.