pycom06g06390

General transcription factor IIH subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0016634
Physical Location & Seq
Forward (+)
10066101 .. 10067225
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g06390.1

Sequence Viewer

Length: 213 bp
ATGGTGAACGCTGTTAAAGGACTGTTCATCTCTTGTGACATACCGATGGCGCAATTCATCATCAATTACGACAATTCACTGCCTGCTTCACAGAGATTCATAATACACGTCTTGGATAGCACTCACCTTTTTGTGCAGCCCCATGCTGCCGAGATGATAAGAAGCGCCATTGCAGAATTCAGAGATCAGAATTCATATGAGAAGCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko03420 map03022 map03420
Pfam Domains
Protein Families
Tfb5

Protein Analysis

71

Amino Acids

7.95

Weight (kDa)

5.78

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tfb5 PF06331 1 - 68 5.7e-27 Transcription factor TFIIH complex subunit Tfb5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016634)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12400 AT1G12400
malus_domestica MD04G1245800.v1.1 MD12G1192500.v1.1
prunus_persica Prupe.5G081100_v2.0.a1
pyrus_communis pycom04g21670 pycom06g06390
rosa_chinensis RchiOBHm_Chr7g0201071
rosa_multiflora Rmu_sc0010648.1_g000001
rosa_roxburghii Rroxscaffold_3G00255540
rosa_samantha Rh7AG187700 Rh7BG191200 Rh7DG193400
rosa_wichuraiana Rw7G016480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 176, 190
AflIII ACRYGT 1 cut(s) 106
AjiI CACGTC 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 136, 146
ApoI RAATTY 2 cut(s) 176, 190
AspLEI GCGC 2 cut(s) 52, 167
AsuHPI GGTGA 2 cut(s) 16, 116
BbvI GCAGC 2 cut(s) 133, 148
BccI CCATC 1 cut(s) 40
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 2 cut(s) 137, 147
BlsI GCNGC 2 cut(s) 138, 148
BmgBI CACGTC 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 133, 148
BsgI GTGCAG 1 cut(s) 155
Bsp143I GATC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 168
BssMI GATC 1 cut(s) 184
Bst4CI ACNGT 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 52, 167
BstKTI GATC 1 cut(s) 187
BstMBI GATC 1 cut(s) 184
BstV1I GCAGC 2 cut(s) 133, 148
BtrI CACGTC 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 2 cut(s) 52, 167
CviAII CATG 1 cut(s) 143
CviJI RGCY 2 cut(s) 139, 205
CviKI_1 RGCY 2 cut(s) 139, 205
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
EcoRI GAATTC 2 cut(s) 176, 190
FaeI CATG 1 cut(s) 146
FaiI YATR 5 cut(s) 41, 101, 144, 196, 198
FatI CATG 1 cut(s) 142
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 137, 147
Fsp4HI GCNGC 2 cut(s) 137, 147
GlaI GCGC 2 cut(s) 51, 166
GluI GCNGC 2 cut(s) 137, 147
HaeII RGCGCY 1 cut(s) 168
HhaI GCGC 2 cut(s) 52, 167
Hin1II CATG 1 cut(s) 146
Hin6I GCGC 2 cut(s) 50, 165
HinP1I GCGC 2 cut(s) 50, 165
HinfI GANTC 1 cut(s) 96
HphI GGTGA 2 cut(s) 16, 116
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 182, 189
Hpy8I GTNNAC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 136, 173
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 1 cut(s) 146
HspAI GCGC 2 cut(s) 50, 165
Kzo9I GATC 1 cut(s) 184
LpnPI CCDG 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 133, 148
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 35
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MluCI AATT 5 cut(s) 53, 64, 73, 176, 190
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 44
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 184
NlaIII CATG 1 cut(s) 146
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 96
PkrI GCNGC 2 cut(s) 138, 148
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 2 cut(s) 137, 147
Sau3AI GATC 1 cut(s) 184
SetI ASST 2 cut(s) 111, 129
SgeI CNNG 6 cut(s) 45, 95, 119, 124, 155, 163
SmiMI CAYNNNNRTG 1 cut(s) 44
Sse9I AATT 5 cut(s) 53, 64, 73, 176, 190
TaaI ACNGT 1 cut(s) 24
TaiI ACGT 1 cut(s) 111
TasI AATT 5 cut(s) 53, 64, 73, 176, 190
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 136, 146
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 4 cut(s) 16, 46, 88, 183
TspRI CASTG 1 cut(s) 84
XapI RAATTY 2 cut(s) 176, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.