MD04G1248100.v1.1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007693
Physical Location & Seq
Forward (+)
32267795 .. 32269096
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1248100.v1.1.491

Sequence Viewer

Length: 999 bp
ATGAAGCAAAATGAGGACGAGTGGCTGGAATACCGCAACGTCATGGAAGACGTCATCAAAAGGAGTCGACTTGACAAGACCCTCTTCTTTGATCTCAGACGACAACTTTGGCGTCCCACAGCTCGGCGGCTCCTTGATTTCTTTTCAAAGTATAGCATCAGTGGCCACTTGGGCAGAAGTCGGAATGTCAACAGTGTCACTCTTCAGGTTTACCTGACATTGAGTTCAACTATAATTGTACGTTCCCAATTCCCATGCCTTGATTACAGATGCTACCGTGCCCTCAAGTTTGATAGCACAATGCCTGTTGGTTTACGAGGTCCGACTAATCTCTTTGCTCATCCAATTGATCAACTATTAGCAGAACTGGATACGCACCTCTCACAGTGGAACTCTAGATCACAAGAACTATTAAGCAAGATTTCCTTCGGGCATTTTCCTCTTTCATTCTCAGCGACCTCAAACTCCGGGAAGAGCCTAAAAGATATATTCCTCAATCATTCCATATCAGCTTATATATGTGGGCATCTGCATTCTAGGTTTGGTAGAAACTTGAAGAGGCGCCATCTGTTGAGTCATCACTTTTTATCGTTGCAGAAGTTTTTCCAGCTTAATGTACATCGGCCGTATTTTCATAGTGCTGTTAACTGTTCAACTGAAGCCCCAGCGGGCAAAGAGTTCTGGGAGTGGGAGATGGGTGAGTGGAAGAGGAGCAGAGCCATGAGAATTTTGGCCATTGATAGAGGCCATGTTTCGTATGTTGACATTGACTTCAAGTCAGGCACCAAAGGACAATCATCTTGCCAACTTTTCCCCTGGACTCACGGTTCACGTCAATATCTTCATCCCATCACGAATATGAATGTCATGATATGGTCCCTTTATCTTACGGCAGTTAGAGCCCTGGTGTTTTCTGTTTCTCCAATTGCATCAATTACGACTAAGATCTATGACTCAAGACCTGGATTTCTCTATCTGGTATTTGACCCATGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

333

Amino Acids

38.67

Weight (kDa)

9.75

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_TMM62 PF24384 296 - 328 6.7e-07 TMEM62 Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AccB1I GGYRCC 2 cut(s) 561, 782
AccI GTMKAC 1 cut(s) 67
AciI CCGC 3 cut(s) 34, 127, 668
AcoI YGGCCR 3 cut(s) 163, 623, 732
AcsI RAATTY 1 cut(s) 726
AcuI CTGAAG 2 cut(s) 188, 678
AcyI GRCGYC 3 cut(s) 51, 112, 562
AfaI GTAC 2 cut(s) 240, 618
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 5 cut(s) 147, 228, 556, 654, 775
AhdI GACNNNNNGTC 1 cut(s) 775
AjiI CACGTC 1 cut(s) 833
AjnI CCWGG 3 cut(s) 815, 903, 961
AloI GAACNNNNNNTCC 2 cut(s) 811, 843
AluBI AGCT 3 cut(s) 122, 512, 610
AluI AGCT 3 cut(s) 122, 512, 610
AoxI GGCC 4 cut(s) 163, 623, 732, 745
ApoI RAATTY 1 cut(s) 726
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
Asp700I GAANNNNTTC 1 cut(s) 602
AspLEI GCGC 1 cut(s) 564
AspS9I GGNCC 2 cut(s) 320, 876
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 710
AvaII GGWCC 2 cut(s) 320, 876
BaeGI GKGCMC 1 cut(s) 283
BalI TGGCCA 2 cut(s) 165, 734
BanI GGYRCC 2 cut(s) 561, 782
BanII GRGCYC 1 cut(s) 904
BbsI GAAGAC 1 cut(s) 54
BccI CCATC 3 cut(s) 573, 688, 857
BceAI ACGGC 2 cut(s) 610, 906
BciT130I CCWGG 3 cut(s) 817, 905, 963
BciVI GTATCC 1 cut(s) 364
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 469
BfaI CTAG 2 cut(s) 396, 537
BfoI RGCGCY 1 cut(s) 565
BfuI GTATCC 1 cut(s) 364
BglI GCCNNNNNGGC 1 cut(s) 171
BglII AGATCT 1 cut(s) 945
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 4 cut(s) 469, 817, 905, 963
Bme18I GGWCC 2 cut(s) 320, 876
BmeRI GACNNNNNGTC 1 cut(s) 775
BmgBI CACGTC 1 cut(s) 833
BmgT120I GGNCC 2 cut(s) 320, 876
BmiI GGNNCC 4 cut(s) 131, 563, 784, 878
BmrFI CCNGG 4 cut(s) 469, 817, 905, 963
BmsI GCATC 4 cut(s) 165, 260, 535, 938
BpiI GAAGAC 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 269, 940
BpuMI CCSGG 1 cut(s) 469
BsaHI GRCGYC 3 cut(s) 51, 112, 562
BsaJI CCNNGG 2 cut(s) 815, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 372
BseBI CCWGG 3 cut(s) 817, 905, 963
BseDI CCNNGG 2 cut(s) 815, 903
BseGI GGATG 2 cut(s) 340, 844
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 109, 465
BseNI ACTGG 1 cut(s) 372
BseRI GAGGAG 1 cut(s) 724
BseSI GKGCMC 1 cut(s) 283
BseX3I CGGCCG 1 cut(s) 623
BseYI CCCAGC 1 cut(s) 664
Bsh1285I CGRYCG 1 cut(s) 626
BshFI GGCC 4 cut(s) 165, 625, 734, 747
BshNI GGYRCC 2 cut(s) 561, 782
BsiEI CGRYCG 1 cut(s) 626
BsiSI CCGG 1 cut(s) 468
BslFI GGGAC 2 cut(s) 99, 862
BslI CCNNNNNNNGG 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 99, 862
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 4 cut(s) 165, 625, 734, 747
Bsp1286I GDGCHC 2 cut(s) 283, 904
Bsp1407I TGTACA 1 cut(s) 616
Bsp143I GATC 4 cut(s) 91, 349, 398, 945
BspACI CCGC 3 cut(s) 34, 127, 668
BspANI GGCC 4 cut(s) 165, 625, 734, 747
BspCNI CTCAG 2 cut(s) 108, 464
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 4 cut(s) 131, 563, 784, 878
BspQI GCTCTTC 1 cut(s) 467
BspT107I GGYRCC 2 cut(s) 561, 782
BsrGI TGTACA 1 cut(s) 616
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 815, 903
BssMI GATC 4 cut(s) 91, 349, 398, 945
BssNI GRCGYC 3 cut(s) 51, 112, 562
Bst2UI CCWGG 3 cut(s) 817, 905, 963
Bst4CI ACNGT 5 cut(s) 194, 278, 387, 650, 827
Bst6I CTCTTC 5 cut(s) 89, 207, 467, 551, 701
BstACI GRCGYC 3 cut(s) 51, 112, 562
BstAUI TGTACA 1 cut(s) 616
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 3 cut(s) 95, 451, 942
BstF5I GGATG 2 cut(s) 340, 844
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 1 cut(s) 564
BstKTI GATC 4 cut(s) 94, 352, 401, 948
BstMBI GATC 4 cut(s) 91, 349, 398, 945
BstMCI CGRYCG 1 cut(s) 626
BstMWI GCNNNNNNNGC 3 cut(s) 162, 171, 899
BstNI CCWGG 3 cut(s) 817, 905, 963
BstSCI CCNGG 4 cut(s) 467, 815, 903, 961
BstSLI GKGCMC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 945
BstYI RGATCY 1 cut(s) 945
BstZI CGGCCG 1 cut(s) 623
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 4 cut(s) 165, 625, 734, 747
BtrI CACGTC 1 cut(s) 833
BtsCI GGATG 2 cut(s) 340, 844
BtsIMutI CAGTG 3 cut(s) 166, 199, 392
Cac8I GCNNGC 1 cut(s) 670
CciI TCATGA 1 cut(s) 867
CfoI GCGC 1 cut(s) 564
Cfr13I GGNCC 2 cut(s) 320, 876
CseI GACGC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 239, 617
CviAII CATG 7 cut(s) 43, 255, 721, 749, 868, 990, 996
CviQI GTAC 2 cut(s) 239, 617
DdeI CTNAG 3 cut(s) 95, 451, 942
DinI GGCGCC 1 cut(s) 563
DpnI GATC 4 cut(s) 93, 351, 400, 947
DpnII GATC 4 cut(s) 91, 349, 398, 945
DriI GACNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 3 cut(s) 163, 623, 732
EagI CGGCCG 1 cut(s) 623
Eam1104I CTCTTC 5 cut(s) 89, 207, 467, 551, 701
Eam1105I GACNNNNNGTC 1 cut(s) 775
EarI CTCTTC 5 cut(s) 89, 207, 467, 551, 701
EclXI CGGCCG 1 cut(s) 623
Eco24I GRGCYC 1 cut(s) 904
Eco47I GGWCC 2 cut(s) 320, 876
Eco52I CGGCCG 1 cut(s) 623
Eco57I CTGAAG 2 cut(s) 188, 678
EcoRII CCWGG 3 cut(s) 815, 903, 961
EcoT38I GRGCYC 1 cut(s) 904
EgeI GGCGCC 1 cut(s) 563
EheI GGCGCC 1 cut(s) 563
FaeI CATG 7 cut(s) 46, 258, 724, 752, 871, 993, 999
FalI AAGNNNNNCTT 4 cut(s) 68, 100, 410, 442
FaqI GGGAC 2 cut(s) 99, 862
FatI CATG 7 cut(s) 42, 254, 720, 748, 867, 989, 995
FauI CCCGC 1 cut(s) 661
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 128
FokI GGATG 2 cut(s) 327, 831
FriOI GRGCYC 1 cut(s) 904
Fsp4HI GCNGC 1 cut(s) 128
FspBI CTAG 2 cut(s) 396, 537
GlaI GCGC 1 cut(s) 563
GluI GCNGC 1 cut(s) 128
GsaI CCCAGC 1 cut(s) 668
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 4 cut(s) 165, 625, 734, 747
HapII CCGG 1 cut(s) 468
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 564
Hin1I GRCGYC 3 cut(s) 51, 112, 562
Hin1II CATG 7 cut(s) 46, 258, 724, 752, 871, 993, 999
Hin6I GCGC 1 cut(s) 562
HinP1I GCGC 1 cut(s) 562
HincII GTYRAC 4 cut(s) 68, 190, 646, 763
HindII GTYRAC 4 cut(s) 68, 190, 646, 763
HinfI GANTC 4 cut(s) 64, 574, 820, 953
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 1 cut(s) 468
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 7 cut(s) 68, 190, 211, 314, 646, 763, 830
Hpy188I TCNGA 3 cut(s) 98, 183, 324
Hpy188III TCNNGA 4 cut(s) 396, 853, 868, 957
Hpy8I GTNNAC 7 cut(s) 68, 190, 211, 314, 646, 763, 830
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 5 cut(s) 194, 278, 387, 650, 827
HpyCH4IV ACGT 4 cut(s) 39, 51, 241, 832
HpyCH4V TGCA 4 cut(s) 532, 595, 929, 993
HpyF10VI GCNNNNNNNGC 3 cut(s) 162, 171, 899
HpyF3I CTNAG 3 cut(s) 95, 451, 942
HpySE526I ACGT 4 cut(s) 39, 51, 241, 832
Hsp92I GRCGYC 3 cut(s) 51, 112, 562
Hsp92II CATG 7 cut(s) 46, 258, 724, 752, 871, 993, 999
HspAI GCGC 1 cut(s) 562
KasI GGCGCC 1 cut(s) 561
Ksp22I TGATCA 1 cut(s) 349
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 4 cut(s) 91, 349, 398, 945
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 2 cut(s) 135, 711
LweI GCATC 4 cut(s) 165, 260, 535, 938
MaeI CTAG 2 cut(s) 396, 537
MaeII ACGT 4 cut(s) 39, 51, 241, 832
MaeIII GTNAC 1 cut(s) 196
MalI GATC 4 cut(s) 93, 351, 400, 947
MboI GATC 4 cut(s) 91, 349, 398, 945
MboII GAAGA 7 cut(s) 59, 76, 194, 484, 568, 718, 833
MfeI CAATTG 2 cut(s) 345, 924
MflI RGATCY 1 cut(s) 945
MhlI GDGCHC 2 cut(s) 283, 904
MlsI TGGCCA 2 cut(s) 165, 734
MluCI AATT 6 cut(s) 234, 248, 345, 726, 924, 933
MluNI TGGCCA 2 cut(s) 165, 734
Mly113I GGCGCC 1 cut(s) 562
MlyI GAGTC 4 cut(s) 73, 583, 814, 947
MmeI TCCRAC 2 cut(s) 161, 347
Mox20I TGGCCA 2 cut(s) 165, 734
MroXI GAANNNNTTC 1 cut(s) 602
MscI TGGCCA 2 cut(s) 165, 734
MseI TTAA 3 cut(s) 413, 612, 645
MslI CAYNNNNRTG 2 cut(s) 857, 994
Msp20I TGGCCA 2 cut(s) 165, 734
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 4 cut(s) 469, 817, 905, 963
MunI CAATTG 2 cut(s) 345, 924
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 3 cut(s) 817, 905, 963
MwoI GCNNNNNNNGC 3 cut(s) 162, 171, 899
NarI GGCGCC 1 cut(s) 562
NciI CCSGG 1 cut(s) 469
NdeII GATC 4 cut(s) 91, 349, 398, 945
NlaIII CATG 7 cut(s) 46, 258, 724, 752, 871, 993, 999
NlaIV GGNNCC 4 cut(s) 131, 563, 784, 878
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 196
PagI TCATGA 1 cut(s) 867
PciSI GCTCTTC 1 cut(s) 467
PctI GAATGC 1 cut(s) 532
PdmI GAANNNNTTC 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 467
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 4 cut(s) 72, 582, 814, 947
PluTI GGCGCC 1 cut(s) 565
PpsI GAGTC 4 cut(s) 72, 582, 814, 947
Psp6I CCWGG 3 cut(s) 815, 903, 961
PspFI CCCAGC 1 cut(s) 664
PspGI CCWGG 3 cut(s) 815, 903, 961
PspN4I GGNNCC 4 cut(s) 131, 563, 784, 878
PspPI GGNCC 2 cut(s) 320, 876
PsuI RGATCY 1 cut(s) 945
RsaI GTAC 2 cut(s) 240, 618
RsaNI GTAC 2 cut(s) 239, 617
RseI CAYNNNNRTG 2 cut(s) 857, 994
SalI GTCGAC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 3 cut(s) 413, 612, 645
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 4 cut(s) 91, 349, 398, 945
Sau96I GGNCC 2 cut(s) 320, 876
SchI GAGTC 4 cut(s) 73, 583, 814, 947
ScrFI CCNGG 4 cut(s) 469, 817, 905, 963
SduI GDGCHC 2 cut(s) 283, 904
SfaNI GCATC 4 cut(s) 165, 260, 535, 938
SfoI GGCGCC 1 cut(s) 563
SinI GGWCC 2 cut(s) 320, 876
SmiMI CAYNNNNRTG 2 cut(s) 857, 994
SmlI CTYRAG 2 cut(s) 284, 955
SmoI CTYRAG 2 cut(s) 284, 955
Sse9I AATT 6 cut(s) 234, 248, 345, 726, 924, 933
SsiI CCGC 3 cut(s) 34, 127, 668
SspDI GGCGCC 1 cut(s) 561
SspMI CTAG 2 cut(s) 396, 537
StyD4I CCNGG 4 cut(s) 467, 815, 903, 961
TaaI ACNGT 5 cut(s) 194, 278, 387, 650, 827
TaiI ACGT 4 cut(s) 42, 54, 244, 835
TaqI TCGA 1 cut(s) 67
TasI AATT 6 cut(s) 234, 248, 345, 726, 924, 933
TatI WGTACW 1 cut(s) 616
TauI GCSGC 1 cut(s) 130
Tru1I TTAA 3 cut(s) 413, 612, 645
Tru9I TTAA 3 cut(s) 413, 612, 645
TscAI CASTG 3 cut(s) 166, 199, 392
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 5 cut(s) 17, 435, 623, 833, 875
TspRI CASTG 3 cut(s) 166, 199, 392
VpaK11BI GGWCC 2 cut(s) 320, 876
XapI RAATTY 1 cut(s) 726
XbaI TCTAGA 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XmiI GTMKAC 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 2 cut(s) 396, 537
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.