Rorug02G0602300

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007693
Physical Location & Seq
Forward (+)
72105505 .. 72116154
10650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0602300.1

Sequence Viewer

Length: 4146 bp
ATGGAGGTTCTTTCATTCTCAATCAATTTGGCTCAAGCTGATTTTGCTTCTATGTTCACTGTTCAATTTCTACTACAAATTAGGACATCACCTTTTCAGGTACTACTTTTCATGATATGGGGATTTAATTGTTGCAAGATTGGATGGGATTCTGCCATGAGAATGAGTGCGAATTTGCGTAATTTATTCTTATATGAGGCCTTTTTGTATTATAACCCACCTCTCATCATGGCCAACATTGGCTATGCAAAAATATTTGATCTTGATCAAAATCATCTCACTCACAAAGAAGTATGGAAGATACTGATGGTTCATTCGATTTTTCATTGTCCTCCCCTCCCCCCATCATCTCTGTATGTGACTATGGGAGCAGCTTGGTTCAATGGTGCTTTTAGCAAGGTGGCAAAGGCAGGGCAGGCTGCAGGCACGAAAACCAGAGAAAAGTTCCATATGGCTGTGTCAAACTTGACTGCAAAGGTAATGCTTACAGACTTTGGCTACATGAAGAAAAGAAGAGGGAAGAAAGGGGAAGGAACAAAGATAGAAGAAAAGAAGGGGGAAGAAAGGGAAGGAGAAGGGAGAGGTGTAGAGATTGGGTCTCTCAAGGGCTGCGACACGAGTTATTGGGAAGACAATGAGAAAGTGTTCAGCGAAATTTTGTGGTGGACAGAATGCAGTGGCGCTATTGTACTTGGCTTGTGGATCATGGGTCAGTTGTTTCCTAGAACTGGTGAGAGACAAGTAGCTAGAATGCGAGTGAGATATTTGAAAGCAGTTCTAAGGCAAGATGTGGGCTACTTTGATCTACATGTCACAAGCACCTCTGAGGTCATCACCAGCATCTCTAGTGATAGCCTCGTGATTCAAGACGTCCTCAGTGAAAAGGTTCCAAACTTTATAATGAACTGCTCTATGTTCTTTGGGAGCTATGTGGCGGCTTTCATAATGCTGTGGAGGCTAGCCATCGTTGCATTTCCGTTTGTGGTGCTTCTGGTAATCCCGGGATTGATTTACGGAAGGATTTTGATGGGACTAGCCAGACAGATTAGTGATGAATACAACAAGGCTGGCACAATAGCAGAACACGCACTGTCATCAATCAGAACCGTTTACGCGTTTGTTGGTGAAAAGAAAGCAATTTCGGAGTTCTCTGCAGCTCTACAAGGCTCAGTGAAGTTGGGATTGAGTCAGGGCCTTGCAAAAGGCTTGGCCATTGGAAGTAACGGCATCGCTTTTGCTATTTGGTCTTTCATGTCCTACTCTGGCAGCAGAATGGTTATGTATCATGGCGCACTTGGAGGCAATGTTATAACAGCTGGTACTGGAATAACCATGGGTGGATTGGCATTCGGGGCAGGGTTGTCAAACCTGAAGTACTTCTCGGAAGCGTGCTCAGCCGCAGAGCGAATATTGGAGGTGATACGGAGAGTCCCCAAGATCGACTCAGACAACATGGAAGGTGAAATGCTAGAGACCGTTTACGGAAAAGTCGAATTCAAGCAGGTTGGCTTCGCCTACCCTTCAAGGCCAGAAAGCATCATCTTCAACAACTTTAACTTGACGGTTCCAGCTGGGAAAACTGTGGCATTGGTGGGTGGTAGTGGCTCGGGAAAGTCGACAGTAATTTCTTTGTTGCAGAGATTTTATGATCCACTTGGAGGTGAGATACTCATTGATGGGGTGGCCATAAAAAATTGTCAGCTCAAGTGGCTTAGGTCCCAGATGGGGCTGGTGAGCCAAGAACCTGCTCTTTTTGCAACCAGCATAAAAGAGAACATACTGTTTGGGAAAGAAGATGCTGACATTGAAGAGGTTATTGAGGCTGGTAAAGCTTCTAATGCTCACGATTTCATCTCTCAGTTGCCTCTGGGATATGACACACAGGTTGGCGAGAAAGGTATTCAAATTTCGGGAGGACAAAAGCAAAGAATAGCCATTGCACGAGCAATCATCAAGAAACCACGAATTCTTCTTCTAGATGAAGCCACCAACGCGTTGGACACAGAATCGGAGTGTATAGTCCAAGAAGCTCTGGACAAAGCAGCGGTGGGACGTACGACGATCATCATCGCCCACCGCCTTTCCACCATCCGAAACGCCGACATTATTGCTCTATTCCAACGTGAGAATGGTCTCTATACCTCGCTTGTCCACTTACAACAAACAGAGAAGCAGAGTGGCCCAGAAGAACACGGACATTACGCCTCGTCATCTATATCAAACCTGGACATTCACAACACAAGAATGTCCAACACAAGCAATCGTAGGCTTTCTATGGTCAGCCGCTCGAATTCTGCCAACTCATTTGCGCAAGGTCGAGCATTATCATTGGTTGCTGGCGAAGATGAGATCGTAGAAGGGCAGAAGCTGCCTGTCCCATCTTTTAGAAGAATGACAGATTTGAACCTTCCGGAATGGAAACAAGCACTTTTGGGGTGCTCCAGTGCTGTACTGTTTGGTGCAGTTCAGCCAGTATACGCATTTGCTATGGGGTCAATGGTGTCGGTTTATTTCTTAACAGATCATGATGAGATCAAGGAGAAGACGAGGATTTACTCACTTTGCTTTCTCGGATTGGCTATTTTCTCTTTGCTTATTAACATCTGCCAGCATTACAACTTTGCCTACATGGGAGAGTACTTGACTAAAAGAGTTAGAGAGAGGATGCTTTCCAAGATTCTTACATTCGAAGTTGGGTGGTTTGATCAAGATGATAATTCTAGTGGTGCCATTTGCTCTAGACTCGCCAAAGATGCCAATGTGGTGAGATCTTTAGTGGGTGATCGGATGGCTCTACTTGTTCAAACTTTCTCAGCTGTGACTATAGCCTGCACAATGGGTTTGGTTATTGCATGGAGACTTGCCTTAGTCATGATAGCAGTAAAACCTATTATCATTGTAAGCATATACACCAGGCTTGTCCTGCTCAAAAGCATGTCCGGGAAAGCCATCAAAGCTCAAGATGAAAGCAGCAAGCTAGCTGCAGAAGCTGTCTCCAACCTCAGAACCATCACTGCCTTCTCATCTCAGGACCGCCTGTTGAAACTGCTCCAAAAGGCCCAAGAAGGCCCACGTAAGGAGAGCATCCGACAATCATGGTTTGGGGGTATAGGGCTCGGCTGTTCTCGAAGCCTTACCTCTATCAGCTGGGCTTTTGACTTTTGGTACGGAGGCAAGCTCGTCGCCAAGGGCTTCGTCACCGCAAAAGCGCTGTTTCAGACTATCATGATATTGGTGAGCACGAGTATGGCCATTGCAGAAGCGGGAAGTATGACTTCGGACCTTGTGAAAGGCTCGGACGCTGTTGCGTCCGTATTTGCCGTGTTGGACCGGTACACAACAATAGAACCCAAAGACCCGGAAGGTTGCGAACCCAAAAGTATAACGGGTCATATAGAACTTCATGACATACATTTTGCGTATCCCACTAGGCCCGATGCAATGATCTTTAATGGATTCTCAATTAATATCGAAGCAAGAAAGTCGACGGCCTTGGTGGGACAAAGTGGATCTGGAAAATCAACAATTATCGGATTAATCGAGAGATTTTATGATCCACTTAAAGGGGAAGTGAGAATTGACGGTCGAAATATAAAGTCGTACCACCTCAGAATGTTAAGGAAACACATAGCGTTGGTTAGCCAAGAGGCAACATTGTTTTCAGGCACCATAAGAGAAAACATCATATACGGCATGTCAGATAAGATTGATGAATTGGAGATAATCGAAGCAGCTAAGGCAGCCAATGCTCATGATTTCATCTCGGGACTCAAGGAGGGATACGACACCTTTTGTGGTTATAGAGGAGTACAACTATCTGGGGGACAAAAGCAACGCATTGCTATAGCCAGAGCTATATTGAGGAACCCGGTGGTGTTACTATTAGATGAATCGACTAGTGCGCTTGATAGCCAATCGGAGAAGGTAGTGCAAGATGCGCTAGAGCGAGTGATGGTGGGGAGGACTAGTGTGGTGGTGGCGCACAGGTTGAGTACCATACAACATTGTGATCTTATCACTGTCCTTGATAAAGGAAGGGTAGTGGAGAGAGGGACTCACTCGTCTCTTTTGGCCAAAGTACCTAAAGGTTCTTACTACTCATTGCTTAGCCTGCAAAGGACGGCATCTGCATCAGAGCTTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

1381

Amino Acids

151.92

Weight (kDa)

8.81

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 228 - 436 5.1e-34 ABC transporter transmembrane region
ABC_tran PF00005 516 - 664 1.1e-33 ABC transporter
ABC_membrane PF00664 811 - 1081 6.1e-49 ABC transporter transmembrane region
ABC_tran PF00005 1151 - 1300 1.7e-30 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 213, 899, 1310
AasI GACNNNNNNGTC 1 cut(s) 4060
AatII GACGTC 1 cut(s) 873
Acc16I TGCGCA 1 cut(s) 2310
Acc36I ACCTGC 2 cut(s) 1492, 1753
AccB1I GGYRCC 2 cut(s) 2726, 3665
AccBSI CCGCTC 1 cut(s) 2286
AccI GTMKAC 3 cut(s) 1616, 2475, 3485
AccII CGCG 2 cut(s) 1115, 1994
AccIII TCCGGA 1 cut(s) 2410
AciI CCGC 8 cut(s) 935, 1398, 2045, 2077, 2284, 3034, 3201, 3263
AclWI GGATC 4 cut(s) 710, 1643, 3517, 3548
AcoI YGGCCR 5 cut(s) 231, 1209, 1683, 3249, 4071
AcsI RAATTY 6 cut(s) 172, 654, 1493, 1905, 1965, 2290
AcuI CTGAAG 1 cut(s) 1391
AcyI GRCGYC 1 cut(s) 870
AfeI AGCGCT 1 cut(s) 3210
AfiI CCNNNNNNNGG 5 cut(s) 728, 1658, 1725, 3076, 3563
AflIII ACRYGT 3 cut(s) 808, 1113, 1992
AgeI ACCGGT 1 cut(s) 3330
AhlI ACTAGT 2 cut(s) 3896, 3965
AjnI CCWGG 2 cut(s) 2223, 2912
Alw21I GWGCWC 3 cut(s) 1394, 2441, 3242
Alw26I GTCTC 7 cut(s) 603, 730, 1466, 2138, 2851, 2998, 4068
AlwI GGATC 4 cut(s) 710, 1643, 3517, 3548
AlwNI CAGNNNCTG 2 cut(s) 2368, 2990
Ama87I CYCGRG 3 cut(s) 1000, 1606, 3763
Aor13HI TCCGGA 1 cut(s) 2410
Aor51HI AGCGCT 1 cut(s) 3210
ApoI RAATTY 6 cut(s) 172, 654, 1493, 1905, 1965, 2290
ArsI GACNNNNNNTTYG 2 cut(s) 3398, 3430
AseI ATTAAT 2 cut(s) 3465, 3536
AsiGI ACCGGT 1 cut(s) 3330
Asp700I GAANNNNTTC 3 cut(s) 644, 885, 1376
AspLEI GCGC 7 cut(s) 683, 1292, 2311, 3211, 3904, 3940, 3982
AspS9I GGNCC 9 cut(s) 1192, 1716, 2180, 3031, 3058, 3068, 3280, 3328, 3432
AsuC2I CCSGG 5 cut(s) 1001, 1002, 2941, 3359, 3869
AsuII TTCGAA 1 cut(s) 2688
AsuNHI GCTAGC 2 cut(s) 958, 2977
AvaI CYCGRG 3 cut(s) 1000, 1606, 3763
AvaII GGWCC 4 cut(s) 1716, 3031, 3280, 3328
BalI TGGCCA 5 cut(s) 233, 1211, 1685, 3251, 4073
BanI GGYRCC 2 cut(s) 2726, 3665
BanII GRGCYC 1 cut(s) 3117
BauI CACGAG 4 cut(s) 616, 857, 1941, 3241
BbsI GAAGAC 2 cut(s) 636, 2549
Bbv12I GWGCWC 3 cut(s) 1394, 2441, 3242
BceAI ACGGC 5 cut(s) 1240, 3305, 3504, 3706, 4137
BcgI CGANNNNNNTGC 6 cut(s) 2090, 2124, 3163, 3197, 3465, 3499
BciT130I CCWGG 2 cut(s) 2225, 2914
BciVI GTATCC 2 cut(s) 3432, 3773
BclI TGATCA 2 cut(s) 265, 2704
BcnI CCSGG 5 cut(s) 1001, 1002, 2941, 3359, 3869
BcoDI GTCTC 7 cut(s) 603, 730, 1466, 2138, 2851, 2998, 4068
BcuI ACTAGT 2 cut(s) 3896, 3965
BfmI CTRYAG 5 cut(s) 420, 1152, 2823, 2982, 3843
BfoI RGCGCY 2 cut(s) 684, 3212
BfuAI ACCTGC 2 cut(s) 1492, 1753
BfuI GTATCC 2 cut(s) 3432, 3773
BglII AGATCT 1 cut(s) 2768
BlpI GCTNAGC 2 cut(s) 1393, 4106
BmcAI AGTACT 2 cut(s) 1376, 2639
Bme1390I CCNGG 7 cut(s) 1001, 1002, 2225, 2914, 2941, 3359, 3869
Bme18I GGWCC 4 cut(s) 1716, 3031, 3280, 3328
BmeT110I CYCGRG 3 cut(s) 1000, 1606, 3763
BmgT120I GGNCC 9 cut(s) 1192, 1716, 2180, 3031, 3058, 3068, 3280, 3328, 3432
BmiI GGNNCC 6 cut(s) 888, 1566, 1718, 2728, 3667, 3866
BmrFI CCNGG 7 cut(s) 1001, 1002, 2225, 2914, 2941, 3359, 3869
BmtI GCTAGC 2 cut(s) 962, 2981
BpiI GAAGAC 2 cut(s) 636, 2549
BplI GAGNNNNNCTC 4 cut(s) 3162, 3194, 4039, 4071
BpmI CTGGAG 1 cut(s) 2425
Bpu10I CCTNAGC 2 cut(s) 1712, 3735
Bpu1102I GCTNAGC 2 cut(s) 1393, 4106
Bpu14I TTCGAA 1 cut(s) 2688
BpuEI CTTGAG 5 cut(s) 18, 587, 1688, 2943, 3755
BpuMI CCSGG 5 cut(s) 1001, 1002, 2941, 3359, 3869
BsaAI YACGTR 1 cut(s) 3074
BsaBI GATNNNNATC 3 cut(s) 264, 270, 2066
BsaHI GRCGYC 1 cut(s) 870
BsaI GGTCTC 3 cut(s) 603, 1466, 2138
BsaJI CCNNGG 4 cut(s) 1000, 1332, 3186, 3492
BsaWI WCCGGW 2 cut(s) 2410, 3330
BsaXI ACNNNNNCTCC 4 cut(s) 1290, 1320, 3952, 3982
Bsc4I CCNNNNNNNGG 5 cut(s) 728, 1658, 1725, 3076, 3563
Bse118I RCCGGY 1 cut(s) 3330
Bse1I ACTGG 4 cut(s) 733, 1327, 2442, 2471
Bse3DI GCAATG 6 cut(s) 1309, 1935, 3252, 3447, 3837, 4100
Bse8I GATNNNNATC 3 cut(s) 264, 270, 2066
BseAI TCCGGA 1 cut(s) 2410
BseBI CCWGG 2 cut(s) 2225, 2914
BseDI CCNNGG 4 cut(s) 1000, 1332, 3186, 3492
BseGI GGATG 5 cut(s) 149, 2088, 2670, 2793, 3084
BseJI GATNNNNATC 3 cut(s) 264, 270, 2066
BseLI CCNNNNNNNGG 5 cut(s) 728, 1658, 1725, 3076, 3563
BseMI GCAATG 6 cut(s) 1309, 1935, 3252, 3447, 3837, 4100
BseNI ACTGG 4 cut(s) 733, 1327, 2442, 2471
BseRI GAGGAG 1 cut(s) 3819
BseYI CCCAGC 2 cut(s) 1571, 3147
BsgI GTGCAG 2 cut(s) 2481, 2815
Bsh1236I CGCG 2 cut(s) 1115, 1994
Bsh1285I CGRYCG 1 cut(s) 3586
BshNI GGYRCC 2 cut(s) 2726, 3665
BshTI ACCGGT 1 cut(s) 3330
BsiEI CGRYCG 1 cut(s) 3586
BsiHKAI GWGCWC 3 cut(s) 1394, 2441, 3242
BsiHKCI CYCGRG 3 cut(s) 1000, 1606, 3763
BsiSI CCGG 6 cut(s) 1001, 2411, 2940, 3331, 3359, 3869
BsiWI CGTACG 1 cut(s) 2054
BslFI GGGAC 9 cut(s) 1044, 1415, 1702, 2064, 2360, 3513, 3780, 3837, 4066
BslI CCNNNNNNNGG 5 cut(s) 728, 1658, 1725, 3076, 3563
BsmAI GTCTC 7 cut(s) 603, 730, 1466, 2138, 2851, 2998, 4068
BsmBI CGTCTC 1 cut(s) 4068
BsmFI GGGAC 9 cut(s) 1044, 1415, 1702, 2064, 2360, 3513, 3780, 3837, 4066
BsmI GAATGC 3 cut(s) 677, 756, 1346
Bso31I GGTCTC 3 cut(s) 603, 1466, 2138
BsoBI CYCGRG 3 cut(s) 1000, 1606, 3763
Bsp119I TTCGAA 1 cut(s) 2688
Bsp1286I GDGCHC 4 cut(s) 1394, 2441, 3117, 3242
Bsp13I TCCGGA 1 cut(s) 2410
Bsp1720I GCTNAGC 2 cut(s) 1393, 4106
Bsp19I CCATGG 1 cut(s) 1332
BspACI CCGC 8 cut(s) 935, 1398, 2045, 2077, 2284, 3034, 3201, 3263
BspEI TCCGGA 1 cut(s) 2410
BspFNI CGCG 2 cut(s) 1115, 1994
BspHI TCATGA 6 cut(s) 111, 2524, 2871, 3225, 3403, 3751
BspLI GGNNCC 6 cut(s) 888, 1566, 1718, 2728, 3667, 3866
BspMAI CTGCAG 3 cut(s) 424, 1156, 2986
BspMI ACCTGC 2 cut(s) 1492, 1753
BspOI GCTAGC 2 cut(s) 962, 2981
BspPI GGATC 4 cut(s) 710, 1643, 3517, 3548
BspT104I TTCGAA 1 cut(s) 2688
BspT107I GGYRCC 2 cut(s) 2726, 3665
BspTNI GGTCTC 3 cut(s) 603, 1466, 2138
BsrBI CCGCTC 1 cut(s) 2286
BsrDI GCAATG 6 cut(s) 1309, 1935, 3252, 3447, 3837, 4100
BsrFI RCCGGY 1 cut(s) 3330
BsrI ACTGG 4 cut(s) 733, 1327, 2442, 2471
BssAI RCCGGY 1 cut(s) 3330
BssECI CCNNGG 4 cut(s) 1000, 1332, 3186, 3492
BssNAI GTATAC 1 cut(s) 2476
BssNI GRCGYC 1 cut(s) 870
BssSI CACGAG 4 cut(s) 616, 857, 1941, 3241
BssT1I CCWWGG 3 cut(s) 1332, 3186, 3492
Bst1107I GTATAC 1 cut(s) 2476
Bst2BI CACGAG 4 cut(s) 616, 857, 1941, 3241
Bst2UI CCWGG 2 cut(s) 2225, 2914
Bst6I CTCTTC 2 cut(s) 508, 1803
BstACI GRCGYC 1 cut(s) 870
BstAPI GCANNNNNTGC 2 cut(s) 2368, 3746
BstBAI YACGTR 1 cut(s) 3074
BstBI TTCGAA 1 cut(s) 2688
BstDSI CCRYGG 1 cut(s) 1332
BstF5I GGATG 5 cut(s) 149, 2088, 2670, 2793, 3084
BstFNI CGCG 2 cut(s) 1115, 1994
BstH2I RGCGCY 2 cut(s) 684, 3212
BstHHI GCGC 7 cut(s) 683, 1292, 2311, 3211, 3904, 3940, 3982
BstMAI GTCTC 7 cut(s) 603, 730, 1466, 2138, 2851, 2998, 4068
BstMCI CGRYCG 1 cut(s) 3586
BstNI CCWGG 2 cut(s) 2225, 2914
BstNSI RCATGY 3 cut(s) 812, 2938, 3697
BstSCI CCNGG 7 cut(s) 999, 1000, 2223, 2912, 2939, 3357, 3867
BstSFI CTRYAG 5 cut(s) 420, 1152, 2823, 2982, 3843
BstUI CGCG 2 cut(s) 1115, 1994
BstV2I GAAGAC 2 cut(s) 636, 2549
BstX2I RGATCY 2 cut(s) 2768, 3509
BstXI CCANNNNNNTGG 1 cut(s) 1996
BstYI RGATCY 2 cut(s) 2768, 3509
BstZ17I GTATAC 1 cut(s) 2476
BsuI GTATCC 2 cut(s) 3432, 3773
BtgI CCRYGG 1 cut(s) 1332
BtgZI GCGATG 2 cut(s) 1213, 2053
BtsCI GGATG 5 cut(s) 149, 2088, 2670, 2793, 3084
BtsI GCAGTG 2 cut(s) 682, 3012
BtsIMutI CAGTG 8 cut(s) 57, 682, 883, 1088, 1176, 2449, 3012, 4017
BveI ACCTGC 2 cut(s) 1492, 1753
CaiI CAGNNNCTG 2 cut(s) 2368, 2990
CciI TCATGA 6 cut(s) 111, 2524, 2871, 3225, 3403, 3751
CfoI GCGC 7 cut(s) 683, 1292, 2311, 3211, 3904, 3940, 3982
Cfr10I RCCGGY 1 cut(s) 3330
Cfr13I GGNCC 9 cut(s) 1192, 1716, 2180, 3031, 3058, 3068, 3280, 3328, 3432
Cfr9I CCCGGG 1 cut(s) 1000
CseI GACGC 2 cut(s) 3297, 3308
CspAI ACCGGT 1 cut(s) 3330
DrdI GACNNNNNNGTC 1 cut(s) 4060
DseDI GACNNNNNNGTC 1 cut(s) 4060
EaeI YGGCCR 5 cut(s) 231, 1209, 1683, 3249, 4071
Eam1104I CTCTTC 2 cut(s) 508, 1803
EarI CTCTTC 2 cut(s) 508, 1803
Eco130I CCWWGG 3 cut(s) 1332, 3186, 3492
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 3117
Eco31I GGTCTC 3 cut(s) 603, 1466, 2138
Eco47I GGWCC 4 cut(s) 1716, 3031, 3280, 3328
Eco47III AGCGCT 1 cut(s) 3210
Eco57I CTGAAG 1 cut(s) 1391
Eco88I CYCGRG 3 cut(s) 1000, 1606, 3763
EcoO109I RGGNCCY 2 cut(s) 1192, 1716
EcoRI GAATTC 3 cut(s) 1493, 1965, 2290
EcoRII CCWGG 2 cut(s) 2223, 2912
EcoT14I CCWWGG 3 cut(s) 1332, 3186, 3492
EcoT38I GRGCYC 1 cut(s) 3117
ErhI CCWWGG 3 cut(s) 1332, 3186, 3492
Esp3I CGTCTC 1 cut(s) 4068
FalI AAGNNNNNCTT 2 cut(s) 3259, 3291
FaqI GGGAC 9 cut(s) 1044, 1415, 1702, 2064, 2360, 3513, 3780, 3837, 4066
FauI CCCGC 1 cut(s) 3256
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 2 cut(s) 265, 2704
FblI GTMKAC 3 cut(s) 1616, 2475, 3485
FokI GGATG 5 cut(s) 156, 2075, 2677, 2800, 3071
FriOI GRGCYC 1 cut(s) 3117
FspI TGCGCA 1 cut(s) 2310
GlaI GCGC 7 cut(s) 682, 1291, 2310, 3210, 3903, 3939, 3981
GsaI CCCAGC 2 cut(s) 1575, 3151
GsuI CTGGAG 1 cut(s) 2425
HaeII RGCGCY 2 cut(s) 684, 3212
HapII CCGG 6 cut(s) 1001, 2411, 2940, 3331, 3359, 3869
HgaI GACGC 2 cut(s) 3297, 3308
HhaI GCGC 7 cut(s) 683, 1292, 2311, 3211, 3904, 3940, 3982
Hin1I GRCGYC 1 cut(s) 870
Hin6I GCGC 7 cut(s) 681, 1290, 2309, 3209, 3902, 3938, 3980
HinP1I GCGC 7 cut(s) 681, 1290, 2309, 3209, 3902, 3938, 3980
HincII GTYRAC 2 cut(s) 1617, 3486
HindII GTYRAC 2 cut(s) 1617, 3486
HindIII AAGCTT 1 cut(s) 1830
HpaII CCGG 6 cut(s) 1001, 2411, 2940, 3331, 3359, 3869
Hpy166II GTNNAC 9 cut(s) 57, 666, 1111, 1480, 1617, 2152, 2476, 3336, 3486
Hpy8I GTNNAC 9 cut(s) 57, 666, 1111, 1480, 1617, 2152, 2476, 3336, 3486
Hpy99I CGWCG 3 cut(s) 2062, 3185, 3490
HpyCH4IV ACGT 4 cut(s) 870, 2053, 2122, 3073
HpySE526I ACGT 4 cut(s) 870, 2053, 2122, 3073
Hsp92I GRCGYC 1 cut(s) 870
HspAI GCGC 7 cut(s) 681, 1290, 2309, 3209, 3902, 3938, 3980
Kpn2I TCCGGA 1 cut(s) 2410
Ksp22I TGATCA 2 cut(s) 265, 2704
LmnI GCTCC 4 cut(s) 368, 924, 2444, 3054
MaeII ACGT 4 cut(s) 870, 2053, 2122, 3073
MaeIII GTNAC 6 cut(s) 358, 811, 1220, 2818, 3196, 3876
MbiI CCGCTC 1 cut(s) 2286
MflI RGATCY 2 cut(s) 2768, 3509
MhlI GDGCHC 4 cut(s) 1394, 2441, 3117, 3242
MlsI TGGCCA 5 cut(s) 233, 1211, 1685, 3251, 4073
MluI ACGCGT 2 cut(s) 1113, 1992
MluNI TGGCCA 5 cut(s) 233, 1211, 1685, 3251, 4073
MlyI GAGTC 6 cut(s) 1195, 1436, 1437, 2736, 3762, 4048
MmeI TCCRAC 6 cut(s) 1977, 2143, 2274, 3021, 3112, 3306
Mox20I TGGCCA 5 cut(s) 233, 1211, 1685, 3251, 4073
MroI TCCGGA 1 cut(s) 2410
MroXI GAANNNNTTC 3 cut(s) 644, 885, 1376
MscI TGGCCA 5 cut(s) 233, 1211, 1685, 3251, 4073
MseI TTAA 9 cut(s) 126, 1554, 2516, 2598, 3450, 3465, 3536, 3561, 3617
MslI CAYNNNNRTG 3 cut(s) 161, 2243, 3245
Msp20I TGGCCA 5 cut(s) 233, 1211, 1685, 3251, 4073
MspA1I CMGCKG 5 cut(s) 1316, 1571, 2045, 2816, 3147
MspI CCGG 6 cut(s) 1001, 2411, 2940, 3331, 3359, 3869
MspR9I CCNGG 7 cut(s) 1001, 1002, 2225, 2914, 2941, 3359, 3869
Mva1269I GAATGC 3 cut(s) 677, 756, 1346
MvaI CCWGG 2 cut(s) 2225, 2914
MvnI CGCG 2 cut(s) 1115, 1994
NciI CCSGG 5 cut(s) 1001, 1002, 2941, 3359, 3869
NcoI CCATGG 1 cut(s) 1332
NdeI CATATG 1 cut(s) 450
NheI GCTAGC 2 cut(s) 958, 2977
NlaIV GGNNCC 6 cut(s) 888, 1566, 1718, 2728, 3667, 3866
NmeAIII GCCGAG 1 cut(s) 3096
NmuCI GTSAC 4 cut(s) 358, 811, 2818, 3196
NsbI TGCGCA 1 cut(s) 2310
NspI RCATGY 3 cut(s) 812, 2938, 3697
NspV TTCGAA 1 cut(s) 2688
PagI TCATGA 6 cut(s) 111, 2524, 2871, 3225, 3403, 3751
PceI AGGCCT 1 cut(s) 200
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 3 cut(s) 1488, 1613, 3537
PctI GAATGC 3 cut(s) 677, 756, 1346
PdmI GAANNNNTTC 3 cut(s) 644, 885, 1376
PfeI GAWTC 6 cut(s) 149, 862, 2006, 2677, 3456, 3890
Pfl23II CGTACG 1 cut(s) 2054
PfoI TCCNGGA 1 cut(s) 2939
PinAI ACCGGT 1 cut(s) 3330
PleI GAGTC 6 cut(s) 1194, 1436, 1436, 2736, 3762, 4048
PpsI GAGTC 6 cut(s) 1194, 1436, 1436, 2736, 3762, 4048
Ppu21I YACGTR 1 cut(s) 3074
PpuMI RGGWCCY 1 cut(s) 1716
PscI ACATGT 1 cut(s) 808
PshBI ATTAAT 2 cut(s) 3465, 3536
PsiI TTATAA 3 cut(s) 213, 899, 1310
Psp5II RGGWCCY 1 cut(s) 1716
Psp6I CCWGG 2 cut(s) 2223, 2912
PspFI CCCAGC 2 cut(s) 1571, 3147
PspGI CCWGG 2 cut(s) 2223, 2912
PspLI CGTACG 1 cut(s) 2054
PspN4I GGNNCC 6 cut(s) 888, 1566, 1718, 2728, 3667, 3866
PspPI GGNCC 9 cut(s) 1192, 1716, 2180, 3031, 3058, 3068, 3280, 3328, 3432
PspPPI RGGWCCY 1 cut(s) 1716
PstI CTGCAG 3 cut(s) 424, 1156, 2986
PstNI CAGNNNCTG 2 cut(s) 2368, 2990
PsuI RGATCY 2 cut(s) 2768, 3509
PvuII CAGCTG 4 cut(s) 1316, 1571, 2816, 3147
RseI CAYNNNNRTG 3 cut(s) 161, 2243, 3245
SalI GTCGAC 2 cut(s) 1615, 3484
SaqAI TTAA 9 cut(s) 126, 1554, 2516, 2598, 3450, 3465, 3536, 3561, 3617
Sau96I GGNCC 9 cut(s) 1192, 1716, 2180, 3031, 3058, 3068, 3280, 3328, 3432
ScaI AGTACT 2 cut(s) 1376, 2639
SchI GAGTC 6 cut(s) 1195, 1436, 1437, 2736, 3762, 4048
ScrFI CCNGG 7 cut(s) 1001, 1002, 2225, 2914, 2941, 3359, 3869
SduI GDGCHC 4 cut(s) 1394, 2441, 3117, 3242
SfcI CTRYAG 5 cut(s) 420, 1152, 2823, 2982, 3843
SfuI TTCGAA 1 cut(s) 2688
SinI GGWCC 4 cut(s) 1716, 3031, 3280, 3328
SmaI CCCGGG 1 cut(s) 1002
SmiMI CAYNNNNRTG 3 cut(s) 161, 2243, 3245
SmlI CTYRAG 5 cut(s) 33, 602, 1703, 2958, 3770
SmoI CTYRAG 5 cut(s) 33, 602, 1703, 2958, 3770
SpeI ACTAGT 2 cut(s) 3896, 3965
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 8 cut(s) 935, 1398, 2045, 2077, 2284, 3034, 3201, 3263
SspI AATATT 2 cut(s) 255, 1410
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 7 cut(s) 999, 1000, 2223, 2912, 2939, 3357, 3867
StyI CCWWGG 3 cut(s) 1332, 3186, 3492
TaiI ACGT 4 cut(s) 873, 2056, 2125, 3076
TatI WGTACW 5 cut(s) 688, 1374, 2449, 2637, 3808
TauI GCSGC 3 cut(s) 938, 1400, 2286
TfiI GAWTC 6 cut(s) 149, 862, 2006, 2677, 3456, 3890
Tru1I TTAA 9 cut(s) 126, 1554, 2516, 2598, 3450, 3465, 3536, 3561, 3617
Tru9I TTAA 9 cut(s) 126, 1554, 2516, 2598, 3450, 3465, 3536, 3561, 3617
TscAI CASTG 8 cut(s) 64, 682, 883, 1095, 1176, 2449, 3019, 4024
TseFI GTSAC 4 cut(s) 358, 811, 2818, 3196
Tsp45I GTSAC 4 cut(s) 358, 811, 2818, 3196
TspGWI ACGGA 7 cut(s) 966, 1029, 1438, 1497, 2208, 3183, 3301
TspMI CCCGGG 1 cut(s) 1000
TspRI CASTG 8 cut(s) 64, 682, 883, 1095, 1176, 2449, 3019, 4024
VpaK11BI GGWCC 4 cut(s) 1716, 3031, 3280, 3328
VspI ATTAAT 2 cut(s) 3465, 3536
XapI RAATTY 6 cut(s) 172, 654, 1493, 1905, 1965, 2290
XbaI TCTAGA 2 cut(s) 1975, 2738
XceI RCATGY 3 cut(s) 812, 2938, 3697
XcmI CCANNNNNNNNNTGG 3 cut(s) 1339, 1993, 2126
XmaI CCCGGG 1 cut(s) 1000
XmiI GTMKAC 3 cut(s) 1616, 2475, 3485
XmnI GAANNNNTTC 3 cut(s) 644, 885, 1376
ZraI GACGTC 1 cut(s) 871
ZrmI AGTACT 2 cut(s) 1376, 2639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.