Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007693

Physical Location & Seq

Genomic Coordinates Reverse (-)

32897403 .. 32900897

Gene Structure 3495 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1262500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGGTGGTGGGTCGAGTGGCATTCTGATGATGGTGTTTAGGGTAATAATGCTCTGCCACTGCCTCCCTCTATATCTCAATCTCAATCTCAATCTGTATGCGGTAGCGGTAGCAGTAACAACGACTGAAGAATCCGAAGAAGAATTCCAACAACAACTGAGGAGGGCGGTAGATGTGAAGGGAGGCCCCGACTCAGTTGTGTGGGTGGTTCAGCTCTCTGATCTCCATTTCAGCGTCCACCATCCCGACAGAGCTCTCGACTTCAAGAATTTTGTGGGTCCCGCCCTCTCCTTCATCAACCCTTCTCTCGTCTTCATCACCGGTGACCTCACAGATGGAAAAAGCAAGGATTTGTTGACAATGAAGCAAAATGAGGACGAGTGGCTGGAATACCACAACGTCATGGAGGACGTCATCAAAAGGAGTCGACTTGACAAGACCCTCTTCTTTGATCTCAGAGGAAATCACGACAACTTTGGCGTGCCACAGCTCGGCGGCTCCTTTGATTTCTTTTCAAAGTACAGCATCAGTGGCCAGTTGGGCAGAAGACGGAATGTCAACAGTGTCACTCTTCAGACTGGTGATCGGAAGCATCTCTTTGTTGGAGTTGATACCACAATGTCTGTTGGTTTACGAGGTCCAACTAATCTCTTTGGGCATCCAACTGATCAACTATTAGCAGAATTGGATACACACCTCTCTCAGTGGGACTCTAGATCACAAGAACCATTAAGCAAGATTTCCTTTGGGCATTTTCCTCTTTCATTCTCAGTGACCTCAAACTCCGGGAAGAGACTAAAAGATATATTCCTCAATCATTCCATATCAGCTTATATATGTGGGCATCTGCATTCTAGCTTTGGCAGAAACTTGAAGAGGCGCCATCAGTTGAGTCGTCACTTTTTATCCTTGCAAAAGTTTTTCCAGCTTAATGTACACCAGCCCTATTTTCATAGTGCTGTTAACTGTTCAACTGAAGCCCCAGCCGCCAAAGAGTTCTGGGAGTGGGAGATGGGTGACTGGAGGAGGAGCAGAGCCATGAGAATTTTGGCCATTGATAGAGGCCATGTTTCGTATGTTGACATTGACTTCAAGTCAGGCACCAAAAAGACTATCATATTGCCAACTTTTCCCCTGGACTCACGGTTCATGTCAACATCTTCATCCCATCACGAGTATGAATGTCATGATATGGTCCCTTTATCTTATGGGACAGTTAGAGCCCTGGTGTTTTCTGTTTCTCCAATTGTATCAGTTACAACTAAGATCTATGACTCAAGACCTGGATTTCTCAATCTGGTATTTGAGGCACCCATGCAAATGATTGGGGATAACACCTCCAGGGGAGATCTTTATGTTGCTCCATGGAATCATAGAGCCTTTGAGGATCCAGTCCCTAGCAGATATTGGTTGCAAATAGAAGCAACAGACTTCATGGGCAGATCAATTTTATCTGATTTGAGGCCATTTTCTGTTAATGGCCTTACTACTATGACTTCATGGACCTGGAAGGAATTTATGGTCATGGGTTGTCAATGGGATGCCTTGTATTACCCAATGCTCTGGTGTGCTGTATATTTCGTACTCTCCATCCTCCTTATTCCAAAAGCTATCCTCACTTTCTCAGTTAGGCATTTCACTTACAAGAATAAGGGCTTCGTGAATGCTGTAGGATTGGTTTTTCAGGAGCTCTGCAGGCTTCCCGTAGCATGGTTTGGTTTTTTAGGATACTTGTTCTACCTTATATTATTCCCCTGGGTTACTGGGCAAGTTTTCACTGACGGCAAGGATAAAGGATACATGACCTATATGGGGTGGGTGGTCAAATCTTTTAATCAGAAGGGAAAACATGAGTATGTTGGATCTCCAGATGTAATGGTAGTGGTTCTTCCACATCTCGTCTTTGTGGTTTTTCCTGCTCTTTTCCTGACTGCAGCTTTGGCTACTGAAAAACAGATCTCCAGAGAACATTTTCTCTCCGTTACAGGGAAGAAAGAAGATGACTATGGTCCAGAGGAAAAGAGATCTTTATGGTACGATTACTCAGGTAATAGGAGATCAAATTCTGGTGTTGGAAACCGGTGGATTCGAAATGTACTTTTGGTTCTTTGCTTTGCAGTTTGTTGGAAGCACTTTATGAATTGCAGGACTCTCATCAAAGCATATGAGATGAATCCCATTCTCCATTTTCCTGGATATAGCCTAACAATTCCACTACTGTTGGCTTATACGGCCTACAAAACCAGGAGTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos

Protein Analysis

752

Amino Acids

85.95

Weight (kDa)

8.73

Isoelectric Point (pI)

43.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Metallophos	PF00149	69 - 285	1.2e-10	Calcineurin-like phosphoesterase
Ig_TMM62	PF24384	368 - 495	1.1e-52	TMEM62 Ig-like domain
TMEM62_C	PF24394	518 - 731	2.3e-89	TMEM62 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007693)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03305
fragaria_vesca	FvH4_6g00140 FvH4_6g00140
malus_domestica	MD04G1248100.v1.1 MD12G1262500.v1.1
prunus_persica	Prupe.6G364500_v2.0.a1 Prupe.6G364500_v2.0.a1 Prupe.6G364500_v2.0.a1 Prupe.6G364500_v2.0.a1 Prupe.6G364500_v2.0.a1 Prupe.6G364500_v2.0.a1 Prupe.6G364500_v2.0.a1
pyrus_communis	pycom04g21870 pycom12g24150
rosa_chinensis	RchiOBHm_Chr3g0447491
rosa_laevigata	RLG00000025993
rosa_multiflora	Rmu_sc0006572.1_g000004
rosa_roxburghii	Rroxscaffold_6G00425710
rosa_rugosa	Rorug02G0602300
rosa_samantha	Rh3AG002500 Rh3BG002000 Rh3CG002300 Rh3DG002400
rosa_wichuraiana	Rw3G000140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	414
AccB1I	GGYRCC	3 cut(s)	879, 1100, 1309
AccI	GTMKAC	1 cut(s)	427
AciI	CCGC	6 cut(s)	101, 107, 167, 282, 495, 987
AclWI	GGATC	3 cut(s)	1382, 1395, 1870
AcoI	YGGCCR	2 cut(s)	532, 1050
AcsI	RAATTY	5 cut(s)	143, 268, 1044, 1514, 2062
AcuI	CTGAAG	3 cut(s)	147, 557, 996
AcyI	GRCGYC	2 cut(s)	411, 880
AfaI	GTAC	5 cut(s)	521, 936, 1584, 2036, 2097
AfiI	CCNNNNNNNGG	4 cut(s)	491, 1710, 1812, 1986
AgeI	ACCGGT	2 cut(s)	320, 2079
AgsI	TTSAA	5 cut(s)	265, 516, 874, 972, 1093
AhdI	GACNNNNNGTC	2 cut(s)	554, 1093
AjnI	CCWGG	8 cut(s)	1134, 1224, 1282, 1340, 1505, 1754, 2191, 2243
AloI	GAACNNNNNNTCC	2 cut(s)	1130, 1162
AluBI	AGCT	9 cut(s)	214, 254, 490, 830, 858, 928, 1610, 1690, 1937
AluI	AGCT	9 cut(s)	214, 254, 490, 830, 858, 928, 1610, 1690, 1937
Alw21I	GWGCWC	2 cut(s)	256, 1692
Alw26I	GTCTC	1 cut(s)	787
AlwI	GGATC	3 cut(s)	1382, 1395, 1870
AoxI	GGCC	7 cut(s)	184, 532, 1050, 1063, 1463, 1480, 2232
ApeKI	GCWGC	1 cut(s)	1934
ApoI	RAATTY	5 cut(s)	143, 268, 1044, 1514, 2062
ArsI	GACNNNNNNTTYG	2 cut(s)	1921, 1953
AsiGI	ACCGGT	2 cut(s)	320, 2079
Asp700I	GAANNNNTTC	1 cut(s)	1971
AspLEI	GCGC	1 cut(s)	882
AspS9I	GGNCC	6 cut(s)	185, 278, 638, 1195, 1503, 2009
AsuC2I	CCSGG	1 cut(s)	787
AsuHPI	GGTGA	4 cut(s)	310, 335, 593, 1028
AsuII	TTCGAA	1 cut(s)	2089
AvaII	GGWCC	5 cut(s)	278, 638, 1195, 1503, 2009
BalI	TGGCCA	2 cut(s)	534, 1052
BamHI	GGATCC	1 cut(s)	1387
BanI	GGYRCC	3 cut(s)	879, 1100, 1309
BanII	GRGCYC	3 cut(s)	256, 1225, 1692
BarI	GAAGNNNNNNTAC	2 cut(s)	1872, 1904
BauI	CACGAG	1 cut(s)	1172
BbsI	GAAGAC	2 cut(s)	304, 553
Bbv12I	GWGCWC	2 cut(s)	256, 1692
BbvI	GCAGC	1 cut(s)	1946
BccI	CCATC	7 cut(s)	25, 249, 329, 891, 1006, 1176, 1598
BceAI	ACGGC	2 cut(s)	1798, 2247
BciT130I	CCWGG	8 cut(s)	1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
BciVI	GTATCC	3 cut(s)	682, 1721, 1790
BclI	TGATCA	1 cut(s)	667
BcnI	CCSGG	1 cut(s)	787
BcoDI	GTCTC	1 cut(s)	787
BfaI	CTAG	3 cut(s)	714, 855, 1398
BfmI	CTRYAG	3 cut(s)	1668, 1693, 1932
BfoI	RGCGCY	1 cut(s)	883
BfuI	GTATCC	3 cut(s)	682, 1721, 1790
BglI	GCCNNNNNGGC	1 cut(s)	540
BglII	AGATCT	4 cut(s)	1266, 1348, 1956, 2024
BisI	GCNGC	3 cut(s)	496, 987, 1935
BlsI	GCNGC	3 cut(s)	497, 988, 1936
Bme1390I	CCNGG	9 cut(s)	787, 1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
Bme18I	GGWCC	5 cut(s)	278, 638, 1195, 1503, 2009
BmeRI	GACNNNNNGTC	2 cut(s)	554, 1093
BmgT120I	GGNCC	6 cut(s)	185, 278, 638, 1195, 1503, 2009
BmiI	GGNNCC	9 cut(s)	187, 279, 280, 499, 881, 1102, 1197, 1311, 1389
BmrFI	CCNGG	9 cut(s)	787, 1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
BmrI	ACTGGG	1 cut(s)	1773
BmsI	GCATC	5 cut(s)	534, 601, 667, 853, 1531
BmuI	ACTGGG	1 cut(s)	1773
BoxI	GACNNNNGTC	1 cut(s)	2007
BpiI	GAAGAC	2 cut(s)	304, 553
BpmI	CTGGAG	4 cut(s)	1042, 1324, 1851, 1945
Bpu14I	TTCGAA	1 cut(s)	2089
BpuEI	CTTGAG	1 cut(s)	1261
BpuMI	CCSGG	1 cut(s)	787
BsaHI	GRCGYC	2 cut(s)	411, 880
BsaJI	CCNNGG	6 cut(s)	1134, 1224, 1341, 1364, 1754, 1755
BsaWI	WCCGGW	2 cut(s)	320, 2079
Bsc4I	CCNNNNNNNGG	4 cut(s)	491, 1710, 1812, 1986
Bse118I	RCCGGY	2 cut(s)	320, 2079
Bse1I	ACTGG	5 cut(s)	535, 583, 1025, 1391, 1768
BseBI	CCWGG	8 cut(s)	1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
BseDI	CCNNGG	6 cut(s)	1134, 1224, 1341, 1364, 1754, 1755
BseGI	GGATG	5 cut(s)	241, 658, 1163, 1546, 1590
BseLI	CCNNNNNNNGG	4 cut(s)	491, 1710, 1812, 1986
BseMII	CTCAG	7 cut(s)	149, 207, 469, 716, 783, 1638, 2058
BseNI	ACTGG	5 cut(s)	535, 583, 1025, 1391, 1768
BseRI	GAGGAG	3 cut(s)	175, 1039, 1042
BseXI	GCAGC	1 cut(s)	1946
BseYI	CCCAGC	1 cut(s)	982
BshFI	GGCC	7 cut(s)	186, 534, 1052, 1065, 1465, 1482, 2234
BshNI	GGYRCC	3 cut(s)	879, 1100, 1309
BshTI	ACCGGT	2 cut(s)	320, 2079
BsiHKAI	GWGCWC	2 cut(s)	256, 1692
BsiSI	CCGG	3 cut(s)	321, 786, 2080
BslFI	GGGAC	5 cut(s)	264, 722, 1181, 1225, 1379
BslI	CCNNNNNNNGG	4 cut(s)	491, 1710, 1812, 1986
BsmAI	GTCTC	1 cut(s)	787
BsmFI	GGGAC	5 cut(s)	264, 722, 1181, 1225, 1379
BsmI	GAATGC	3 cut(s)	21, 850, 1669
BsnI	GGCC	7 cut(s)	186, 534, 1052, 1065, 1465, 1482, 2234
Bsp119I	TTCGAA	1 cut(s)	2089
Bsp1286I	GDGCHC	3 cut(s)	256, 1225, 1692
Bsp1407I	TGTACA	1 cut(s)	934
Bsp19I	CCATGG	1 cut(s)	1364
BspACI	CCGC	6 cut(s)	101, 107, 167, 282, 495, 987
BspANI	GGCC	7 cut(s)	186, 534, 1052, 1065, 1465, 1482, 2234
BspCNI	CTCAG	7 cut(s)	150, 206, 468, 715, 782, 1637, 2057
BspHI	TCATGA	1 cut(s)	1186
BspLI	GGNNCC	9 cut(s)	187, 279, 280, 499, 881, 1102, 1197, 1311, 1389
BspMAI	CTGCAG	2 cut(s)	1697, 1936
BspPI	GGATC	3 cut(s)	1382, 1395, 1870
BspT104I	TTCGAA	1 cut(s)	2089
BspT107I	GGYRCC	3 cut(s)	879, 1100, 1309
BsrFI	RCCGGY	2 cut(s)	320, 2079
BsrGI	TGTACA	1 cut(s)	934
BsrI	ACTGG	5 cut(s)	535, 583, 1025, 1391, 1768
BssAI	RCCGGY	2 cut(s)	320, 2079
BssECI	CCNNGG	6 cut(s)	1134, 1224, 1341, 1364, 1754, 1755
BssNI	GRCGYC	2 cut(s)	411, 880
BssSI	CACGAG	1 cut(s)	1172
BssT1I	CCWWGG	1 cut(s)	1364
Bst2BI	CACGAG	1 cut(s)	1172
Bst2UI	CCWGG	8 cut(s)	1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
Bst4CI	ACNGT	5 cut(s)	563, 968, 1146, 1216, 2220
Bst6I	CTCTTC	4 cut(s)	449, 576, 785, 869
BstACI	GRCGYC	2 cut(s)	411, 880
BstAUI	TGTACA	1 cut(s)	934
BstBI	TTCGAA	1 cut(s)	2089
BstC8I	GCNNGC	2 cut(s)	482, 1697
BstDEI	CTNAG	8 cut(s)	158, 193, 455, 702, 769, 1263, 1624, 2044
BstDSI	CCRYGG	1 cut(s)	1364
BstEII	GGTNACC	1 cut(s)	323
BstF5I	GGATG	5 cut(s)	241, 658, 1163, 1546, 1590
BstH2I	RGCGCY	1 cut(s)	883
BstHHI	GCGC	1 cut(s)	882
BstMAI	GTCTC	1 cut(s)	787
BstMWI	GCNNNNNNNGC	6 cut(s)	531, 540, 986, 1696, 1940, 2231
BstNI	CCWGG	8 cut(s)	1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
BstPAI	GACNNNNGTC	1 cut(s)	2007
BstPI	GGTNACC	1 cut(s)	323
BstSCI	CCNGG	9 cut(s)	785, 1134, 1224, 1282, 1340, 1505, 1754, 2191, 2243
BstSFI	CTRYAG	3 cut(s)	1668, 1693, 1932
BstV1I	GCAGC	1 cut(s)	1946
BstV2I	GAAGAC	2 cut(s)	304, 553
BstX2I	RGATCY	6 cut(s)	1266, 1348, 1387, 1862, 1956, 2024
BstXI	CCANNNNNNTGG	2 cut(s)	1563, 2192
BstYI	RGATCY	6 cut(s)	1266, 1348, 1387, 1862, 1956, 2024
BsuI	GTATCC	3 cut(s)	682, 1721, 1790
BsuRI	GGCC	7 cut(s)	186, 534, 1052, 1065, 1465, 1482, 2234
BtgI	CCRYGG	1 cut(s)	1364
BtsCI	GGATG	5 cut(s)	241, 658, 1163, 1546, 1590
BtsI	GCAGTG	1 cut(s)	58
BtsIMutI	CAGTG	6 cut(s)	58, 535, 568, 710, 777, 1776
Cac8I	GCNNGC	2 cut(s)	482, 1697
CciI	TCATGA	1 cut(s)	1186
CfoI	GCGC	1 cut(s)	882
Cfr10I	RCCGGY	2 cut(s)	320, 2079
Cfr13I	GGNCC	6 cut(s)	185, 278, 638, 1195, 1503, 2009
CseI	GACGC	1 cut(s)	223
Csp6I	GTAC	5 cut(s)	520, 935, 1583, 2035, 2096
CspAI	ACCGGT	2 cut(s)	320, 2079
CspCI	CAANNNNNGTGG	2 cut(s)	2202, 2237
CviQI	GTAC	5 cut(s)	520, 935, 1583, 2035, 2096
DdeI	CTNAG	8 cut(s)	158, 193, 455, 702, 769, 1263, 1624, 2044
DinI	GGCGCC	1 cut(s)	881
DriI	GACNNNNNGTC	2 cut(s)	554, 1093
EaeI	YGGCCR	2 cut(s)	532, 1050
Eam1104I	CTCTTC	4 cut(s)	449, 576, 785, 869
Eam1105I	GACNNNNNGTC	2 cut(s)	554, 1093
EarI	CTCTTC	4 cut(s)	449, 576, 785, 869
Ecl136II	GAGCTC	2 cut(s)	254, 1690
Eco130I	CCWWGG	1 cut(s)	1364
Eco24I	GRGCYC	3 cut(s)	256, 1225, 1692
Eco47I	GGWCC	5 cut(s)	278, 638, 1195, 1503, 2009
Eco53kI	GAGCTC	2 cut(s)	254, 1690
Eco57I	CTGAAG	3 cut(s)	147, 557, 996
Eco91I	GGTNACC	1 cut(s)	323
EcoICRI	GAGCTC	2 cut(s)	254, 1690
EcoO109I	RGGNCCY	2 cut(s)	185, 278
EcoO65I	GGTNACC	1 cut(s)	323
EcoRI	GAATTC	1 cut(s)	143
EcoRII	CCWGG	8 cut(s)	1134, 1224, 1282, 1340, 1505, 1754, 2191, 2243
EcoT14I	CCWWGG	1 cut(s)	1364
EcoT38I	GRGCYC	3 cut(s)	256, 1225, 1692
EgeI	GGCGCC	1 cut(s)	881
EheI	GGCGCC	1 cut(s)	881
ErhI	CCWWGG	1 cut(s)	1364
FalI	AAGNNNNNCTT	6 cut(s)	428, 460, 581, 613, 728, 760
FaqI	GGGAC	5 cut(s)	264, 722, 1181, 1225, 1379
FauI	CCCGC	1 cut(s)	289
FauNDI	CATATG	1 cut(s)	2164
FbaI	TGATCA	1 cut(s)	667
FblI	GTMKAC	1 cut(s)	427
Fnu4HI	GCNGC	3 cut(s)	496, 987, 1935
FokI	GGATG	5 cut(s)	228, 645, 1150, 1553, 1577
FriOI	GRGCYC	3 cut(s)	256, 1225, 1692
Fsp4HI	GCNGC	3 cut(s)	496, 987, 1935
FspBI	CTAG	3 cut(s)	714, 855, 1398
GlaI	GCGC	1 cut(s)	881
GluI	GCNGC	3 cut(s)	496, 987, 1935
GsaI	CCCAGC	1 cut(s)	986
GsuI	CTGGAG	4 cut(s)	1042, 1324, 1851, 1945
HaeII	RGCGCY	1 cut(s)	883
HaeIII	GGCC	7 cut(s)	186, 534, 1052, 1065, 1465, 1482, 2234
HapII	CCGG	3 cut(s)	321, 786, 2080
HgaI	GACGC	1 cut(s)	223
HhaI	GCGC	1 cut(s)	882
Hin1I	GRCGYC	2 cut(s)	411, 880
Hin6I	GCGC	1 cut(s)	880
HinP1I	GCGC	1 cut(s)	880
HincII	GTYRAC	6 cut(s)	357, 428, 559, 964, 1081, 1155
HindII	GTYRAC	6 cut(s)	357, 428, 559, 964, 1081, 1155
HpaI	GTTAAC	1 cut(s)	964
HpaII	CCGG	3 cut(s)	321, 786, 2080
HphI	GGTGA	4 cut(s)	310, 335, 593, 1028
Hpy166II	GTNNAC	9 cut(s)	238, 357, 428, 559, 632, 937, 964, 1081, 1155
Hpy188I	TCNGA	8 cut(s)	27, 136, 220, 458, 576, 588, 1456, 1839
Hpy8I	GTNNAC	9 cut(s)	238, 357, 428, 559, 632, 937, 964, 1081, 1155
HpyAV	CCTTC	5 cut(s)	172, 301, 312, 1504, 1834
HpyCH4III	ACNGT	5 cut(s)	563, 968, 1146, 1216, 2220
HpyCH4IV	ACGT	2 cut(s)	399, 411
HpyCH4V	TGCA	8 cut(s)	850, 913, 1318, 1414, 1695, 1934, 2117, 2145
HpyF10VI	GCNNNNNNNGC	6 cut(s)	531, 540, 986, 1696, 1940, 2231
HpyF3I	CTNAG	8 cut(s)	158, 193, 455, 702, 769, 1263, 1624, 2044
HpySE526I	ACGT	2 cut(s)	399, 411
Hsp92I	GRCGYC	2 cut(s)	411, 880
HspAI	GCGC	1 cut(s)	880
KasI	GGCGCC	1 cut(s)	879
KflI	GGGWCCC	1 cut(s)	278
Ksp22I	TGATCA	1 cut(s)	667
KspAI	GTTAAC	1 cut(s)	964
LmnI	GCTCC	4 cut(s)	503, 1029, 1366, 1687
Lsp1109I	GCAGC	1 cut(s)	1946
LweI	GCATC	5 cut(s)	534, 601, 667, 853, 1531
MaeI	CTAG	3 cut(s)	714, 855, 1398
MaeII	ACGT	2 cut(s)	399, 411
MaeIII	GTNAC	9 cut(s)	115, 323, 565, 772, 896, 1016, 1255, 1759, 1981
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	6 cut(s)	1266, 1348, 1387, 1862, 1956, 2024
MhlI	GDGCHC	3 cut(s)	256, 1225, 1692
MlsI	TGGCCA	2 cut(s)	534, 1052
MluNI	TGGCCA	2 cut(s)	534, 1052
Mly113I	GGCGCC	1 cut(s)	880
MlyI	GAGTC	7 cut(s)	185, 433, 704, 901, 1133, 1268, 2143
MmeI	TCCRAC	7 cut(s)	172, 583, 665, 686, 1840, 2053, 2105
Mox20I	TGGCCA	2 cut(s)	534, 1052
MroXI	GAANNNNTTC	1 cut(s)	1971
MscI	TGGCCA	2 cut(s)	534, 1052
MseI	TTAA	5 cut(s)	731, 930, 963, 1476, 1833
MslI	CAYNNNNRTG	4 cut(s)	26, 1176, 1319, 1854
Msp20I	TGGCCA	2 cut(s)	534, 1052
MspI	CCGG	3 cut(s)	321, 786, 2080
MspR9I	CCNGG	9 cut(s)	787, 1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	3 cut(s)	21, 850, 1669
MvaI	CCWGG	8 cut(s)	1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
MwoI	GCNNNNNNNGC	6 cut(s)	531, 540, 986, 1696, 1940, 2231
NarI	GGCGCC	1 cut(s)	880
NciI	CCSGG	1 cut(s)	787
NcoI	CCATGG	1 cut(s)	1364
NdeI	CATATG	1 cut(s)	2164
NlaIV	GGNNCC	9 cut(s)	187, 279, 280, 499, 881, 1102, 1197, 1311, 1389
NmeAIII	GCCGAG	1 cut(s)	471
NmuCI	GTSAC	5 cut(s)	323, 565, 772, 896, 1016
NspV	TTCGAA	1 cut(s)	2089
PagI	TCATGA	1 cut(s)	1186
PasI	CCCWGGG	1 cut(s)	1755
PctI	GAATGC	3 cut(s)	21, 850, 1669
PdmI	GAANNNNTTC	1 cut(s)	1971
PfeI	GAWTC	4 cut(s)	131, 1369, 2086, 2173
PfoI	TCCNGGA	2 cut(s)	785, 2191
PinAI	ACCGGT	2 cut(s)	320, 2079
PkrI	GCNGC	3 cut(s)	497, 988, 1936
PleI	GAGTC	7 cut(s)	185, 432, 704, 900, 1133, 1268, 2143
PluTI	GGCGCC	1 cut(s)	883
PpsI	GAGTC	7 cut(s)	185, 432, 704, 900, 1133, 1268, 2143
PpuMI	RGGWCCY	1 cut(s)	278
PshAI	GACNNNNGTC	1 cut(s)	2007
Psp124BI	GAGCTC	2 cut(s)	256, 1692
Psp5II	RGGWCCY	1 cut(s)	278
Psp6I	CCWGG	8 cut(s)	1134, 1224, 1282, 1340, 1505, 1754, 2191, 2243
PspEI	GGTNACC	1 cut(s)	323
PspFI	CCCAGC	1 cut(s)	982
PspGI	CCWGG	8 cut(s)	1134, 1224, 1282, 1340, 1505, 1754, 2191, 2243
PspN4I	GGNNCC	9 cut(s)	187, 279, 280, 499, 881, 1102, 1197, 1311, 1389
PspPI	GGNCC	6 cut(s)	185, 278, 638, 1195, 1503, 2009
PspPPI	RGGWCCY	1 cut(s)	278
PsrI	GAACNNNNNNTAC	2 cut(s)	2088, 2120
PstI	CTGCAG	2 cut(s)	1697, 1936
PsuI	RGATCY	6 cut(s)	1266, 1348, 1387, 1862, 1956, 2024
RsaI	GTAC	5 cut(s)	521, 936, 1584, 2036, 2097
RsaNI	GTAC	5 cut(s)	520, 935, 1583, 2035, 2096
RseI	CAYNNNNRTG	4 cut(s)	26, 1176, 1319, 1854
SacI	GAGCTC	2 cut(s)	256, 1692
SalI	GTCGAC	1 cut(s)	426
SaqAI	TTAA	5 cut(s)	731, 930, 963, 1476, 1833
SatI	GCNGC	3 cut(s)	496, 987, 1935
Sau96I	GGNCC	6 cut(s)	185, 278, 638, 1195, 1503, 2009
SchI	GAGTC	7 cut(s)	185, 433, 704, 901, 1133, 1268, 2143
ScrFI	CCNGG	9 cut(s)	787, 1136, 1226, 1284, 1342, 1507, 1756, 2193, 2245
SduI	GDGCHC	3 cut(s)	256, 1225, 1692
SfaNI	GCATC	5 cut(s)	534, 601, 667, 853, 1531
SfcI	CTRYAG	3 cut(s)	1668, 1693, 1932
SfoI	GGCGCC	1 cut(s)	881
SfuI	TTCGAA	1 cut(s)	2089
SgrAI	CRCCGGYG	1 cut(s)	320
SinI	GGWCC	5 cut(s)	278, 638, 1195, 1503, 2009
SmiMI	CAYNNNNRTG	4 cut(s)	26, 1176, 1319, 1854
SmlI	CTYRAG	1 cut(s)	1276
SmoI	CTYRAG	1 cut(s)	1276
SsiI	CCGC	6 cut(s)	101, 107, 167, 282, 495, 987
SspDI	GGCGCC	1 cut(s)	879
SspMI	CTAG	3 cut(s)	714, 855, 1398
SstI	GAGCTC	2 cut(s)	256, 1692
StyD4I	CCNGG	9 cut(s)	785, 1134, 1224, 1282, 1340, 1505, 1754, 2191, 2243
StyI	CCWWGG	1 cut(s)	1364
TaaI	ACNGT	5 cut(s)	563, 968, 1146, 1216, 2220
TaiI	ACGT	2 cut(s)	402, 414
TaqI	TCGA	4 cut(s)	14, 258, 427, 2089
TatI	WGTACW	3 cut(s)	519, 934, 2095
TauI	GCSGC	2 cut(s)	498, 989
TfiI	GAWTC	4 cut(s)	131, 1369, 2086, 2173
Tru1I	TTAA	5 cut(s)	731, 930, 963, 1476, 1833
Tru9I	TTAA	5 cut(s)	731, 930, 963, 1476, 1833
TscAI	CASTG	6 cut(s)	65, 535, 568, 710, 777, 1783
TseFI	GTSAC	5 cut(s)	323, 565, 772, 896, 1016
TseI	GCWGC	1 cut(s)	1934
Tsp45I	GTSAC	5 cut(s)	323, 565, 772, 896, 1016
TspGWI	ACGGA	2 cut(s)	565, 1969
TspRI	CASTG	6 cut(s)	65, 535, 568, 710, 777, 1783
VpaK11BI	GGWCC	5 cut(s)	278, 638, 1195, 1503, 2009
XapI	RAATTY	5 cut(s)	143, 268, 1044, 1514, 2062
XbaI	TCTAGA	1 cut(s)	713
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1045
XmiI	GTMKAC	1 cut(s)	427
XmnI	GAANNNNTTC	1 cut(s)	1971
XspI	CTAG	3 cut(s)	714, 855, 1398
ZraI	GACGTC	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.