Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010524

Physical Location & Seq

Genomic Coordinates Reverse (-)

5334692 .. 5338988

Gene Structure 4297 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1032700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGGCTGCGTTCAGGCCAAGCCTCTCACGAACTCCACTCCTGGGGGGCTAGAGAAGCTCAAACTGGATAATGGGTACGTCGCAAATGGTCCCCTTAACGGTCACAGACGATCAACTGGCCAGTTCCATAGGGACCCCTTTAAGCCGCGGCAGCAGAGGGGTGAGGCCAGAATTTCAAATGTCGAGCCTGGCAGTGGAGGAAGCAGCAACGAAAAGGCGGCGGCAGCGGTGGCGGATGAGTGGGGGGCGTTGAATGGTGGTAGTGAGGGAGAGAAAGATGATGGGAGTCACAAAGTTTCTCATAAAGTTTCTGTGGATGATGAGGAGTTTGTGGATGGATGGCCCAAGTGGCTCACTGATAATATTTCGAGAGAGATTTTGGCTGGCTTGGTTCCGAAGAGTGCTGACTCTTTTGACAAGCTTGATAAGGTTGGCGAAGGAACCTACAGTAATGTGTATAAGGCGCGAGACAGGGACTCTGGAAAAATTGTTGCCTTGAAGAAGGTCCGGTTTGATACATCAGAACCCGAGAGTGTTAAATTTATGGCACGAGAGATTATGTTTTTACGGAAATTGGATCATCCCAATGTGGTCAAGCTTGAAGGATTGGCTACGTCAAGAATGCAGTACAGCATTTATCTTGTTTTTGGTTTCATGGTGTCAGATTTGACAAGAATTATTTCCAGCCCTGAAGGGCTTACTGAACCACAGGTCAAGTGCTATATGCATCAACTACTTTCAGGTCTGCAGCATTGTCACGAGAGGGGAATTATACATCGAGACATCAAAGGATCAAACCTATTGATAGATAAAAGTGGAATGCTAAAAATTGCAGATTTTGGGCTTGCAAACTATTATGTTCAAAGCCCTAAGCGCCCACTCACAAACCGAGTTGTGACGCTTTGGTACAGAGCTCCTGAGCTACTGTTAGGTTCTACAGATTATGGAGTTGGCATTGATCTTTGGAGTGCTGGTTGTGTCTTGGCTGAAATGTTTACAGGGAGACCACTTCTGCCTGGTAGAACAGAGGTTGAGCAACTTCATAGGATTTTCAGGCTTTGTGGCACTCCGTCAGAGGAATACTGGAAAAGATTGAAGCTGTCTACAACTTTTAGACCTCCACGTTCATACAAACCCAGTCTTCAAGAAGCTTTCAAGGGTTTCCCTATTTCTTCTTTGGGTCTGTTGAGCACTCTTCTTGCTTTAGATCCTGCTTATCGTGGCTCTGCATCTTCGGCTCTCAGAAACGAATTCTTTTTCAGAAGCCCCTTAGCATGTGAACTTTCTGGTCTACCTATAATCTACAATGAGGATGAAGAACTGAAATTAGCCAATGAGCAGAAAAAGTCTAGGAATTCTAAAGTAAGACGGTCTCGAACACGTGAGTGCCAGAGACAAGATGCATCTGCTGAGAAAGTAAAAGAAACTTGTGCGTCTTCCAACCAGCCAACCTATCATGCATGTAAGAGGAAGGGAAAAGAAAAGATTCAAATAAAAAGAAAAGAAGGAAGAAGAAACGACAATGTCAGGGAGGAGCCTCGTCTAGCCCTTCTCACCTATACTGTTTATTCAAATTTTGAAAGTGAGCCAGGTAGCACTACCAGTAGCAACTCCTCCAGTGCAAACCAGGCAGGCCGAAAAGAAAGCCCAATCTTCTCCCTCTCACCAGTTGAAGGCCTTTCGGGTGCTACCAAGTACGTCAAGAACCTACCCCCATTACCCAAATCTAAAGCGCGTGCCACCAGCAAATACAATCAGATTAACAGGTCTACATCCACTAGAGAGTTCAGAAGGTTCAATCCAAGGGAGCTAGAACACTATGCTCTTGATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

612

Amino Acids

68.38

Weight (kDa)

9.41

Isoelectric Point (pI)

42.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	139 - 419	2.3e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	141 - 349	4.8e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010524)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090
malus_domestica	MD05G1032700.v1.1 MD10G1033600.v1.1
prunus_persica	Prupe.8G039300_v2.0.a1 Prupe.8G039300_v2.0.a1 Prupe.8G039300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0274351
rosa_laevigata	RLG00000013550
rosa_multiflora	Rmu_ssc0000361.1_g000017
rosa_roxburghii	Rroxscaffold_7G00194310
rosa_rugosa	Rorug06G0081100
rosa_samantha	Rh6AG196300 Rh6BG200500 Rh6CG201400 Rh6DG192200
rosa_wichuraiana	Rw6G017150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	1103, 1291, 1769
AccII	CGCG	3 cut(s)	148, 466, 1735
AciI	CCGC	6 cut(s)	146, 148, 218, 221, 227, 233
AclWI	GGATC	3 cut(s)	585, 799, 1202
AcoI	YGGCCR	1 cut(s)	118
AcsI	RAATTY	5 cut(s)	171, 539, 1250, 1354, 1573
AcuI	CTGAAG	1 cut(s)	711
AcvI	CACGTG	1 cut(s)	1382
AfaI	GTAC	4 cut(s)	77, 629, 908, 1697
AfiI	CCNNNNNNNGG	4 cut(s)	42, 98, 194, 1673
AflIII	ACRYGT	1 cut(s)	1379
AjnI	CCWGG	5 cut(s)	40, 187, 1015, 1588, 1626
AleI	CACNNNNGTG	1 cut(s)	1384
AluBI	AGCT	8 cut(s)	58, 421, 598, 914, 922, 1099, 1151, 1810
AluI	AGCT	8 cut(s)	58, 421, 598, 914, 922, 1099, 1151, 1810
Alw21I	GWGCWC	2 cut(s)	916, 1193
Alw26I	GTCTC	5 cut(s)	462, 774, 997, 1377, 1387
AlwI	GGATC	3 cut(s)	585, 799, 1202
Ama87I	CYCGRG	1 cut(s)	527
AoxI	GGCC	6 cut(s)	15, 118, 165, 341, 1633, 1675
ApeKI	GCWGC	5 cut(s)	6, 151, 204, 224, 748
ApoI	RAATTY	5 cut(s)	171, 539, 1250, 1354, 1573
Asp700I	GAANNNNTTC	2 cut(s)	679, 1485
AspLEI	GCGC	3 cut(s)	466, 876, 1735
AspS9I	GGNCC	4 cut(s)	89, 133, 342, 505
AsuHPI	GGTGA	3 cut(s)	173, 1546, 1656
AvaI	CYCGRG	1 cut(s)	527
AvaII	GGWCC	3 cut(s)	89, 133, 505
BalI	TGGCCA	1 cut(s)	120
BanII	GRGCYC	1 cut(s)	916
BauI	CACGAG	2 cut(s)	549, 758
BbrPI	CACGTG	1 cut(s)	1382
BbsI	GAAGAC	2 cut(s)	1133, 1428
Bbv12I	GWGCWC	2 cut(s)	916, 1193
BbvI	GCAGC	4 cut(s)	163, 216, 236, 760
BccI	CCATC	3 cut(s)	275, 329, 333
BciT130I	CCWGG	5 cut(s)	42, 189, 1017, 1590, 1628
BcoDI	GTCTC	5 cut(s)	462, 774, 997, 1377, 1387
BfaI	CTAG	5 cut(s)	50, 1350, 1544, 1779, 1811
BfmI	CTRYAG	3 cut(s)	445, 746, 936
BfoI	RGCGCY	1 cut(s)	877
BglI	GCCNNNNNGGC	1 cut(s)	349
BisI	GCNGC	9 cut(s)	7, 146, 149, 152, 205, 219, 222, 225, 749
BlsI	GCNGC	9 cut(s)	8, 147, 150, 153, 206, 220, 223, 226, 750
Bme1390I	CCNGG	5 cut(s)	42, 189, 1017, 1590, 1628
Bme18I	GGWCC	3 cut(s)	89, 133, 505
BmeT110I	CYCGRG	1 cut(s)	527
BmgT120I	GGNCC	4 cut(s)	89, 133, 342, 505
BmiI	GGNNCC	6 cut(s)	91, 134, 135, 393, 442, 1536
BmrFI	CCNGG	5 cut(s)	42, 189, 1017, 1590, 1628
BmrI	ACTGGG	1 cut(s)	1131
BmsI	GCATC	4 cut(s)	736, 1238, 1390, 1412
BmuI	ACTGGG	1 cut(s)	1131
BpiI	GAAGAC	2 cut(s)	1133, 1428
BpmI	CTGGAG	1 cut(s)	1600
Bpu10I	CCTNAGC	3 cut(s)	870, 918, 1270
BsaAI	YACGTR	1 cut(s)	1382
BsaI	GGTCTC	2 cut(s)	997, 1377
BsaJI	CCNNGG	3 cut(s)	41, 146, 1802
BsaWI	WCCGGW	1 cut(s)	507
BsaXI	ACNNNNNCTCC	2 cut(s)	958, 988
Bsc4I	CCNNNNNNNGG	4 cut(s)	42, 98, 194, 1673
Bse1I	ACTGG	8 cut(s)	69, 121, 121, 1088, 1137, 1602, 1617, 1667
BseBI	CCWGG	5 cut(s)	42, 189, 1017, 1590, 1628
BseDI	CCNNGG	3 cut(s)	41, 146, 1802
BseGI	GGATG	7 cut(s)	241, 322, 340, 344, 580, 1318, 1772
BseLI	CCNNNNNNNGG	4 cut(s)	42, 98, 194, 1673
BseMII	CTCAG	3 cut(s)	909, 1255, 1401
BseNI	ACTGG	8 cut(s)	69, 121, 121, 1088, 1137, 1602, 1617, 1667
BseRI	GAGGAG	3 cut(s)	338, 1547, 1603
BseXI	GCAGC	4 cut(s)	163, 216, 236, 760
Bsh1236I	CGCG	3 cut(s)	148, 466, 1735
BshFI	GGCC	6 cut(s)	17, 120, 167, 343, 1635, 1677
BsiHKAI	GWGCWC	2 cut(s)	916, 1193
BsiHKCI	CYCGRG	1 cut(s)	527
BsiSI	CCGG	1 cut(s)	508
BslFI	GGGAC	3 cut(s)	75, 146, 488
BslI	CCNNNNNNNGG	4 cut(s)	42, 98, 194, 1673
BsmAI	GTCTC	5 cut(s)	462, 774, 997, 1377, 1387
BsmFI	GGGAC	3 cut(s)	75, 146, 488
BsmI	GAATGC	2 cut(s)	627, 825
BsnI	GGCC	6 cut(s)	17, 120, 167, 343, 1635, 1677
Bso31I	GGTCTC	2 cut(s)	997, 1377
BsoBI	CYCGRG	1 cut(s)	527
Bsp1286I	GDGCHC	2 cut(s)	916, 1193
Bsp143I	GATC	5 cut(s)	110, 577, 791, 958, 1207
BspACI	CCGC	6 cut(s)	146, 148, 218, 221, 227, 233
BspANI	GGCC	6 cut(s)	17, 120, 167, 343, 1635, 1677
BspCNI	CTCAG	3 cut(s)	910, 1254, 1402
BspFNI	CGCG	3 cut(s)	148, 466, 1735
BspLI	GGNNCC	6 cut(s)	91, 134, 135, 393, 442, 1536
BspMAI	CTGCAG	1 cut(s)	750
BspPI	GGATC	3 cut(s)	585, 799, 1202
BspTNI	GGTCTC	2 cut(s)	997, 1377
BsrI	ACTGG	8 cut(s)	69, 121, 121, 1088, 1137, 1602, 1617, 1667
BssECI	CCNNGG	3 cut(s)	41, 146, 1802
BssMI	GATC	5 cut(s)	110, 577, 791, 958, 1207
BssSI	CACGAG	2 cut(s)	549, 758
BssT1I	CCWWGG	1 cut(s)	1802
Bst2BI	CACGAG	2 cut(s)	549, 758
Bst2UI	CCWGG	5 cut(s)	42, 189, 1017, 1590, 1628
Bst4CI	ACNGT	5 cut(s)	101, 449, 927, 1371, 1564
Bst6I	CTCTTC	2 cut(s)	392, 1200
BstBAI	YACGTR	1 cut(s)	1382
BstC8I	GCNNGC	4 cut(s)	385, 846, 1633, 1737
BstDEI	CTNAG	5 cut(s)	870, 918, 1241, 1270, 1410
BstDSI	CCRYGG	1 cut(s)	146
BstF5I	GGATG	7 cut(s)	241, 322, 340, 344, 580, 1318, 1772
BstFNI	CGCG	3 cut(s)	148, 466, 1735
BstH2I	RGCGCY	1 cut(s)	877
BstHHI	GCGC	3 cut(s)	466, 876, 1735
BstKTI	GATC	5 cut(s)	113, 580, 794, 961, 1210
BstMAI	GTCTC	5 cut(s)	462, 774, 997, 1377, 1387
BstMBI	GATC	5 cut(s)	110, 577, 791, 958, 1207
BstMWI	GCNNNNNNNGC	8 cut(s)	55, 151, 224, 230, 349, 873, 1235, 1628
BstNI	CCWGG	5 cut(s)	42, 189, 1017, 1590, 1628
BstNSI	RCATGY	2 cut(s)	1278, 1464
BstSCI	CCNGG	5 cut(s)	40, 187, 1015, 1588, 1626
BstSFI	CTRYAG	3 cut(s)	445, 746, 936
BstUI	CGCG	3 cut(s)	148, 466, 1735
BstV1I	GCAGC	4 cut(s)	163, 216, 236, 760
BstV2I	GAAGAC	2 cut(s)	1133, 1428
BstX2I	RGATCY	1 cut(s)	1207
BstYI	RGATCY	1 cut(s)	1207
BsuRI	GGCC	6 cut(s)	17, 120, 167, 343, 1635, 1677
BtgI	CCRYGG	1 cut(s)	146
BtsCI	GGATG	7 cut(s)	241, 322, 340, 344, 580, 1318, 1772
BtsI	GCAGTG	1 cut(s)	199
BtsIMutI	CAGTG	3 cut(s)	199, 354, 1624
Cac8I	GCNNGC	4 cut(s)	385, 846, 1633, 1737
CfoI	GCGC	3 cut(s)	466, 876, 1735
Cfr13I	GGNCC	4 cut(s)	89, 133, 342, 505
Cfr42I	CCGCGG	1 cut(s)	149
CseI	GACGC	2 cut(s)	907, 1422
Csp6I	GTAC	4 cut(s)	76, 628, 907, 1696
CviAII	CATG	4 cut(s)	655, 1275, 1457, 1461
CviQI	GTAC	4 cut(s)	76, 628, 907, 1696
DdeI	CTNAG	5 cut(s)	870, 918, 1241, 1270, 1410
DpnI	GATC	5 cut(s)	112, 579, 793, 960, 1209
DpnII	GATC	5 cut(s)	110, 577, 791, 958, 1207
EaeI	YGGCCR	1 cut(s)	118
Eam1104I	CTCTTC	2 cut(s)	392, 1200
EarI	CTCTTC	2 cut(s)	392, 1200
EciI	GGCGGA	1 cut(s)	248
Ecl136II	GAGCTC	1 cut(s)	914
Eco130I	CCWWGG	1 cut(s)	1802
Eco147I	AGGCCT	1 cut(s)	1677
Eco24I	GRGCYC	1 cut(s)	916
Eco31I	GGTCTC	2 cut(s)	997, 1377
Eco47I	GGWCC	3 cut(s)	89, 133, 505
Eco53kI	GAGCTC	1 cut(s)	914
Eco57I	CTGAAG	1 cut(s)	711
Eco72I	CACGTG	1 cut(s)	1382
Eco88I	CYCGRG	1 cut(s)	527
EcoICRI	GAGCTC	1 cut(s)	914
EcoO109I	RGGNCCY	1 cut(s)	133
EcoRI	GAATTC	2 cut(s)	1250, 1354
EcoRII	CCWGG	5 cut(s)	40, 187, 1015, 1588, 1626
EcoT14I	CCWWGG	1 cut(s)	1802
EcoT22I	ATGCAT	3 cut(s)	729, 1405, 1462
EcoT38I	GRGCYC	1 cut(s)	916
ErhI	CCWWGG	1 cut(s)	1802
FaeI	CATG	4 cut(s)	658, 1278, 1460, 1464
FaqI	GGGAC	3 cut(s)	75, 146, 488
FatI	CATG	4 cut(s)	654, 1274, 1456, 1460
FblI	GTMKAC	3 cut(s)	1103, 1291, 1769
Fnu4HI	GCNGC	9 cut(s)	7, 146, 149, 152, 205, 219, 222, 225, 749
FokI	GGATG	7 cut(s)	248, 329, 347, 351, 567, 1325, 1759
FriOI	GRGCYC	1 cut(s)	916
Fsp4HI	GCNGC	9 cut(s)	7, 146, 149, 152, 205, 219, 222, 225, 749
FspBI	CTAG	5 cut(s)	50, 1350, 1544, 1779, 1811
GlaI	GCGC	3 cut(s)	465, 875, 1734
GluI	GCNGC	9 cut(s)	7, 146, 149, 152, 205, 219, 222, 225, 749
GsuI	CTGGAG	1 cut(s)	1600
HaeII	RGCGCY	1 cut(s)	877
HaeIII	GGCC	6 cut(s)	17, 120, 167, 343, 1635, 1677
HapII	CCGG	1 cut(s)	508
HgaI	GACGC	2 cut(s)	907, 1422
HhaI	GCGC	3 cut(s)	466, 876, 1735
Hin1II	CATG	4 cut(s)	658, 1278, 1460, 1464
Hin6I	GCGC	3 cut(s)	464, 874, 1733
HinP1I	GCGC	3 cut(s)	464, 874, 1733
HindIII	AAGCTT	3 cut(s)	419, 596, 1149
HinfI	GANTC	4 cut(s)	286, 407, 476, 1486
HpaII	CCGG	1 cut(s)	508
HphI	GGTGA	3 cut(s)	173, 1546, 1656
Hpy166II	GTNNAC	5 cut(s)	996, 1104, 1280, 1292, 1770
Hpy188I	TCNGA	8 cut(s)	396, 523, 664, 1075, 1244, 1262, 1758, 1790
Hpy8I	GTNNAC	5 cut(s)	996, 1104, 1280, 1292, 1770
Hpy99I	CGWCG	1 cut(s)	83
HpyAV	CCTTC	9 cut(s)	431, 496, 596, 686, 1465, 1499, 1559, 1667, 1785
HpyCH4III	ACNGT	5 cut(s)	101, 449, 927, 1371, 1564
HpyCH4IV	ACGT	5 cut(s)	78, 614, 1123, 1381, 1698
HpyCH4V	TGCA	9 cut(s)	625, 727, 748, 833, 848, 1229, 1403, 1460, 1622
HpyF10VI	GCNNNNNNNGC	8 cut(s)	55, 151, 224, 230, 349, 873, 1235, 1628
HpyF3I	CTNAG	5 cut(s)	870, 918, 1241, 1270, 1410
HpySE526I	ACGT	5 cut(s)	78, 614, 1123, 1381, 1698
Hsp92II	CATG	4 cut(s)	658, 1278, 1460, 1464
HspAI	GCGC	3 cut(s)	464, 874, 1733
KflI	GGGWCCC	1 cut(s)	133
KspI	CCGCGG	1 cut(s)	149
Kzo9I	GATC	5 cut(s)	110, 577, 791, 958, 1207
LmnI	GCTCC	3 cut(s)	919, 1534, 1807
Lsp1109I	GCAGC	4 cut(s)	163, 216, 236, 760
LweI	GCATC	4 cut(s)	736, 1238, 1390, 1412
MaeI	CTAG	5 cut(s)	50, 1350, 1544, 1779, 1811
MaeII	ACGT	5 cut(s)	78, 614, 1123, 1381, 1698
MaeIII	GTNAC	4 cut(s)	101, 287, 755, 895
MalI	GATC	5 cut(s)	112, 579, 793, 960, 1209
MboI	GATC	5 cut(s)	110, 577, 791, 958, 1207
MflI	RGATCY	1 cut(s)	1207
MhlI	GDGCHC	2 cut(s)	916, 1193
MlsI	TGGCCA	1 cut(s)	120
MluNI	TGGCCA	1 cut(s)	120
MlyI	GAGTC	3 cut(s)	295, 401, 470
MmeI	TCCRAC	1 cut(s)	1464
Mox20I	TGGCCA	1 cut(s)	120
Mph1103I	ATGCAT	3 cut(s)	729, 1405, 1462
MroXI	GAANNNNTTC	2 cut(s)	679, 1485
MscI	TGGCCA	1 cut(s)	120
MseI	TTAA	4 cut(s)	96, 141, 537, 1761
MslI	CAYNNNNRTG	2 cut(s)	585, 1384
Msp20I	TGGCCA	1 cut(s)	120
MspA1I	CMGCKG	2 cut(s)	148, 227
MspI	CCGG	1 cut(s)	508
MspR9I	CCNGG	5 cut(s)	42, 189, 1017, 1590, 1628
Mva1269I	GAATGC	2 cut(s)	627, 825
MvaI	CCWGG	5 cut(s)	42, 189, 1017, 1590, 1628
MvnI	CGCG	3 cut(s)	148, 466, 1735
MwoI	GCNNNNNNNGC	8 cut(s)	55, 151, 224, 230, 349, 873, 1235, 1628
NdeII	GATC	5 cut(s)	110, 577, 791, 958, 1207
NlaIII	CATG	4 cut(s)	658, 1278, 1460, 1464
NlaIV	GGNNCC	6 cut(s)	91, 134, 135, 393, 442, 1536
NmuCI	GTSAC	4 cut(s)	101, 287, 755, 895
NsiI	ATGCAT	3 cut(s)	729, 1405, 1462
NspI	RCATGY	2 cut(s)	1278, 1464
OliI	CACNNNNGTG	1 cut(s)	1384
PceI	AGGCCT	1 cut(s)	1677
PctI	GAATGC	2 cut(s)	627, 825
PdmI	GAANNNNTTC	2 cut(s)	679, 1485
PfeI	GAWTC	1 cut(s)	1486
PflFI	GACNNNGTC	1 cut(s)	1523
PkrI	GCNGC	9 cut(s)	8, 147, 150, 153, 206, 220, 223, 226, 750
PleI	GAGTC	3 cut(s)	294, 401, 470
PmaCI	CACGTG	1 cut(s)	1382
PmlI	CACGTG	1 cut(s)	1382
PpsI	GAGTC	3 cut(s)	294, 401, 470
Ppu21I	YACGTR	1 cut(s)	1382
PpuMI	RGGWCCY	1 cut(s)	133
Psp124BI	GAGCTC	1 cut(s)	916
Psp5II	RGGWCCY	1 cut(s)	133
Psp6I	CCWGG	5 cut(s)	40, 187, 1015, 1588, 1626
PspCI	CACGTG	1 cut(s)	1382
PspGI	CCWGG	5 cut(s)	40, 187, 1015, 1588, 1626
PspN4I	GGNNCC	6 cut(s)	91, 134, 135, 393, 442, 1536
PspPI	GGNCC	4 cut(s)	89, 133, 342, 505
PspPPI	RGGWCCY	1 cut(s)	133
PstI	CTGCAG	1 cut(s)	750
PsuI	RGATCY	1 cut(s)	1207
PsyI	GACNNNGTC	1 cut(s)	1523
RsaI	GTAC	4 cut(s)	77, 629, 908, 1697
RsaNI	GTAC	4 cut(s)	76, 628, 907, 1696
RseI	CAYNNNNRTG	2 cut(s)	585, 1384
SacI	GAGCTC	1 cut(s)	916
SacII	CCGCGG	1 cut(s)	149
SaqAI	TTAA	4 cut(s)	96, 141, 537, 1761
SatI	GCNGC	9 cut(s)	7, 146, 149, 152, 205, 219, 222, 225, 749
Sau3AI	GATC	5 cut(s)	110, 577, 791, 958, 1207
Sau96I	GGNCC	4 cut(s)	89, 133, 342, 505
SchI	GAGTC	3 cut(s)	295, 401, 470
ScrFI	CCNGG	5 cut(s)	42, 189, 1017, 1590, 1628
SduI	GDGCHC	2 cut(s)	916, 1193
SfaNI	GCATC	4 cut(s)	736, 1238, 1390, 1412
SfcI	CTRYAG	3 cut(s)	445, 746, 936
Sfr303I	CCGCGG	1 cut(s)	149
SgrBI	CCGCGG	1 cut(s)	149
SinI	GGWCC	3 cut(s)	89, 133, 505
SmiMI	CAYNNNNRTG	2 cut(s)	585, 1384
SseBI	AGGCCT	1 cut(s)	1677
SsiI	CCGC	6 cut(s)	146, 148, 218, 221, 227, 233
SspI	AATATT	1 cut(s)	364
SspMI	CTAG	5 cut(s)	50, 1350, 1544, 1779, 1811
SstI	GAGCTC	1 cut(s)	916
StuI	AGGCCT	1 cut(s)	1677
StyD4I	CCNGG	5 cut(s)	40, 187, 1015, 1588, 1626
StyI	CCWWGG	1 cut(s)	1802
TaaI	ACNGT	5 cut(s)	101, 449, 927, 1371, 1564
TaiI	ACGT	5 cut(s)	81, 617, 1126, 1384, 1701
TaqI	TCGA	4 cut(s)	183, 368, 778, 1375
TatI	WGTACW	1 cut(s)	627
TauI	GCSGC	4 cut(s)	148, 151, 221, 224
TfiI	GAWTC	1 cut(s)	1486
Tru1I	TTAA	4 cut(s)	96, 141, 537, 1761
Tru9I	TTAA	4 cut(s)	96, 141, 537, 1761
TscAI	CASTG	3 cut(s)	199, 361, 1624
TseFI	GTSAC	4 cut(s)	101, 287, 755, 895
TseI	GCWGC	5 cut(s)	6, 151, 204, 224, 748
Tsp45I	GTSAC	4 cut(s)	101, 287, 755, 895
TspDTI	ATGAA	4 cut(s)	643, 1031, 1116, 1329
TspGWI	ACGGA	2 cut(s)	583, 1059
TspRI	CASTG	3 cut(s)	199, 361, 1624
Tth111I	GACNNNGTC	1 cut(s)	1523
VpaK11BI	GGWCC	3 cut(s)	89, 133, 505
XapI	RAATTY	5 cut(s)	171, 539, 1250, 1354, 1573
XceI	RCATGY	2 cut(s)	1278, 1464
XmiI	GTMKAC	3 cut(s)	1103, 1291, 1769
XmnI	GAANNNNTTC	2 cut(s)	679, 1485
XspI	CTAG	5 cut(s)	50, 1350, 1544, 1779, 1811
Zsp2I	ATGCAT	3 cut(s)	729, 1405, 1462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.