Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010524

Physical Location & Seq

Genomic Coordinates Reverse (-)

4355292 .. 4359104

Gene Structure 3813 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1033600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGGAATAGTTCAGGCCAAGCCTCTCACCAGCTCCGCACCTGGTGGGCTAGAGAAGCTCAAACTGGATAATGGGCACGTCGCAAAAGGGCCGCTTAATGGTCACAGACGATCAACTGGACAGTTCCATAGGGACCCTTTTAAGCCGCAGCAGCAGAGGGGTGAGGCCAGAGTTTCAAATGTCGAGCATGGCAGTGGCGGCAGCAGCAATGAAAAGGCGACGGCGGCGGATGAGGGGGCGGCGTTGACCGGTGGTAGCGAGGGAGAGAAAGATGATGGGAGTCAGAGAGTTTCTTATAAAGTTTCTGTGGATGAGGAGTTGGTGGATGGATGGCCCAAGTGGCTCACTGATAATATTTCGAGGGAGATTTTGGCTGGCTTGGTTCCCAAGAGTGCTGACTCTTACGACAAGCTCGATAAGGTGGGTGAAGGAACCTACAGCAATGTGTATAAGGCTCGAGACAGGGACACTGGAAAAATCGTTGCCTTGAAGAAGGTCCGGTTTGATACATCGGAACCTGAGAGTGTTAAATTTATGGCACGAGAGATTATGTTTTTACGGAAACTGGATCATCCCAATGTAGTCAAGCTTGAAGGATTGGCTACCTCAAGAATGCAGTACAGCATTTATCTTGTTTTTGATTTCATGGTGTCAGATTTGACAAGAATTATTTCCAGCCCTGAAGGGCTTACTGAACCACAGGTCAAGTGCTATATGCATCAATTGCTTTCAGGCATGCAGCACTGTCACGAGAGGGGAATTATACATCGAGACATCAAGGGATCAAACCTATTGTTAGATAAAAGTGGAATGCTAAAAATTGCAGATTTTGGGCTTGCAAACTATTATGTGCAAAGCCCTAAGCGCTCTCTCACAAACCGAGTTGTGACACTTTGGTACAGGGCGCCTGAGCTACTGTTAGGTTCTACAGATTATGGAGTTGGCATTGATCTTTGGAGTGCTGGTTGTGTCTTGGCTGAAATGTTTACAGGGAGACCACTTCTGCCTGGTAGAACAGAGGTTGAGCAACTTCATAGGATTTTCAGGCTTTGTGGCACACCATCAGAGGACTACTGGAAAAGACTGAAGCTATCTACAACTTTTAGACCTCCACGTTCGTACAAACCCAATCTTCAAGAAGCTTTCAAGGATTTCCCTATTTCTTCATTGGGTCTGTTGAGCACTCTCCTTGCTTTAGATCCTGCCTACCGTGGCTCTGCATATTCGGCTCTGAAAAACGAATTCTTTTTCAGAAGCCCCTTAGCATGTGAACTTTCTGGTCTACCTGTAATCTACAATGCGGATGATGAATTGAAACTAGCCAATGAGCATAAAAAGTCTAGGAAAGTAAGGCGGTCTCGAACACGCGAGCGCCAGAGACAAGATGTATCTGCTGAGAAATTAAAAGAAATTTCTGCATCTTCCAACCGGGGCCTACAGGAGCAGGAGAAAACCGTTGATTCAAATTTCGAAGGTGAGCCAGGAAGCACGACAAGTAGCAACTCCTCCAGTGCAAACCAGGAAGGCCGGAAGGAAAGCCCGATCTTCTCCCTCTCCCCAGTCGAAGGTCTTTCAAGTGTTACTAAGAATGTCAAGAACCTACCTCCATTACCCAAGTCTAAAGCGCGAGCCACAAAAGACAATCAGATTAACAGGTCTGCATCCAACAGAGAATTCAGAAGGTTCAATCCAAGGGAGCTAGAACTCTATGCTCGTGATGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

575

Amino Acids

64.1

Weight (kDa)

9.24

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	135 - 416	5.4e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	139 - 345	1.1e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010524)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090
malus_domestica	MD05G1032700.v1.1 MD10G1033600.v1.1
prunus_persica	Prupe.8G039300_v2.0.a1 Prupe.8G039300_v2.0.a1 Prupe.8G039300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0274351
rosa_laevigata	RLG00000013550
rosa_multiflora	Rmu_ssc0000361.1_g000017
rosa_roxburghii	Rroxscaffold_7G00194310
rosa_rugosa	Rorug06G0081100
rosa_samantha	Rh6AG196300 Rh6BG200500 Rh6CG201400 Rh6DG192200
rosa_wichuraiana	Rw6G017150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	297
AccB1I	GGYRCC	1 cut(s)	904
AccI	GTMKAC	1 cut(s)	1282
AccII	CGCG	2 cut(s)	1368, 1627
AciI	CCGC	9 cut(s)	36, 92, 146, 198, 224, 227, 239, 1301, 1354
AclWI	GGATC	3 cut(s)	576, 790, 1193
AcsI	RAATTY	5 cut(s)	530, 1241, 1410, 1465, 1673
AcuI	CTGAAG	2 cut(s)	702, 1106
AcyI	GRCGYC	1 cut(s)	905
AdeI	CACNNNGTG	1 cut(s)	44
AfaI	GTAC	3 cut(s)	620, 899, 1121
AfeI	AGCGCT	1 cut(s)	866
AfiI	CCNNNNNNNGG	2 cut(s)	98, 1565
AgeI	ACCGGT	1 cut(s)	248
AgsI	TTSAA	9 cut(s)	177, 490, 593, 1136, 1147, 1315, 1464, 1575, 1687
AjiI	CACGTC	1 cut(s)	79
AjnI	CCWGG	4 cut(s)	40, 1006, 1480, 1518
AjuI	GAANNNNNNNTTGG	2 cut(s)	1658, 1690
AluBI	AGCT	8 cut(s)	33, 58, 412, 589, 913, 1090, 1142, 1699
AluI	AGCT	8 cut(s)	33, 58, 412, 589, 913, 1090, 1142, 1699
Alw21I	GWGCWC	1 cut(s)	1184
Alw26I	GTCTC	5 cut(s)	453, 765, 988, 1362, 1372
AlwI	GGATC	3 cut(s)	576, 790, 1193
Ama87I	CYCGRG	1 cut(s)	456
Aor51HI	AGCGCT	1 cut(s)	866
AoxI	GGCC	6 cut(s)	15, 89, 165, 332, 1432, 1525
ApeKI	GCWGC	5 cut(s)	148, 151, 201, 204, 739
ApoI	RAATTY	5 cut(s)	530, 1241, 1410, 1465, 1673
AsiGI	ACCGGT	1 cut(s)	248
Asp700I	GAANNNNTTC	1 cut(s)	670
AspLEI	GCGC	4 cut(s)	867, 907, 1374, 1627
AspS9I	GGNCC	5 cut(s)	89, 133, 333, 496, 1432
AsuC2I	CCSGG	1 cut(s)	1430
AsuHPI	GGTGA	4 cut(s)	19, 173, 437, 1487
AsuII	TTCGAA	1 cut(s)	1470
AvaI	CYCGRG	1 cut(s)	456
AvaII	GGWCC	2 cut(s)	133, 496
BaeGI	GKGCMC	1 cut(s)	78
BanI	GGYRCC	1 cut(s)	904
BauI	CACGAG	3 cut(s)	540, 749, 1713
Bbv12I	GWGCWC	1 cut(s)	1184
BbvI	GCAGC	5 cut(s)	160, 163, 213, 216, 751
BccI	CCATC	4 cut(s)	269, 320, 324, 1069
BceAI	ACGGC	1 cut(s)	237
BciT130I	CCWGG	4 cut(s)	42, 1008, 1482, 1520
BcnI	CCSGG	1 cut(s)	1430
BcoDI	GTCTC	5 cut(s)	453, 765, 988, 1362, 1372
BfaI	CTAG	5 cut(s)	50, 1319, 1341, 1700, 1723
BfmI	CTRYAG	3 cut(s)	436, 927, 1436
BfoI	RGCGCY	3 cut(s)	868, 908, 1375
BglI	GCCNNNNNGGC	1 cut(s)	340
Bme1390I	CCNGG	5 cut(s)	42, 1008, 1430, 1482, 1520
Bme18I	GGWCC	2 cut(s)	133, 496
BmeT110I	CYCGRG	1 cut(s)	456
BmgBI	CACGTC	1 cut(s)	79
BmgT120I	GGNCC	5 cut(s)	89, 133, 333, 496, 1432
BmiI	GGNNCC	7 cut(s)	134, 135, 384, 433, 516, 906, 1433
BmrFI	CCNGG	5 cut(s)	42, 1008, 1430, 1482, 1520
BmrI	ACTGGG	1 cut(s)	1553
BmsI	GCATC	3 cut(s)	727, 1427, 1670
BmuI	ACTGGG	1 cut(s)	1553
BpmI	CTGGAG	1 cut(s)	1492
Bpu10I	CCTNAGC	3 cut(s)	861, 909, 1261
Bpu14I	TTCGAA	1 cut(s)	1470
BpuEI	CTTGAG	1 cut(s)	592
BpuMI	CCSGG	1 cut(s)	1430
BsaHI	GRCGYC	1 cut(s)	905
BsaI	GGTCTC	2 cut(s)	988, 1362
BsaJI	CCNNGG	3 cut(s)	1210, 1429, 1691
BsaWI	WCCGGW	2 cut(s)	248, 498
BsaXI	ACNNNNNCTCC	2 cut(s)	949, 979
Bsc4I	CCNNNNNNNGG	2 cut(s)	98, 1565
Bse118I	RCCGGY	1 cut(s)	248
Bse1I	ACTGG	7 cut(s)	69, 121, 475, 570, 1079, 1509, 1559
Bse3DI	GCAATG	2 cut(s)	214, 448
BseBI	CCWGG	4 cut(s)	42, 1008, 1482, 1520
BseDI	CCNNGG	3 cut(s)	1210, 1429, 1691
BseGI	GGATG	7 cut(s)	235, 316, 331, 335, 571, 1309, 1661
BseLI	CCNNNNNNNGG	2 cut(s)	98, 1565
BseMI	GCAATG	2 cut(s)	214, 448
BseMII	CTCAG	3 cut(s)	510, 900, 1386
BseNI	ACTGG	7 cut(s)	69, 121, 475, 570, 1079, 1509, 1559
BseRI	GAGGAG	2 cut(s)	329, 1495
BseSI	GKGCMC	1 cut(s)	78
BseXI	GCAGC	5 cut(s)	160, 163, 213, 216, 751
Bsh1236I	CGCG	2 cut(s)	1368, 1627
BshFI	GGCC	6 cut(s)	17, 91, 167, 334, 1434, 1527
BshNI	GGYRCC	1 cut(s)	904
BshTI	ACCGGT	1 cut(s)	248
BsiHKAI	GWGCWC	1 cut(s)	1184
BsiHKCI	CYCGRG	1 cut(s)	456
BsiSI	CCGG	4 cut(s)	249, 499, 1429, 1528
BslFI	GGGAC	2 cut(s)	146, 479
BslI	CCNNNNNNNGG	2 cut(s)	98, 1565
BsmAI	GTCTC	5 cut(s)	453, 765, 988, 1362, 1372
BsmFI	GGGAC	2 cut(s)	146, 479
BsmI	GAATGC	2 cut(s)	618, 816
BsnI	GGCC	6 cut(s)	17, 91, 167, 334, 1434, 1527
Bso31I	GGTCTC	2 cut(s)	988, 1362
BsoBI	CYCGRG	1 cut(s)	456
Bsp119I	TTCGAA	1 cut(s)	1470
Bsp1286I	GDGCHC	2 cut(s)	78, 1184
Bsp143I	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
BspACI	CCGC	9 cut(s)	36, 92, 146, 198, 224, 227, 239, 1301, 1354
BspANI	GGCC	6 cut(s)	17, 91, 167, 334, 1434, 1527
BspCNI	CTCAG	3 cut(s)	511, 901, 1387
BspFNI	CGCG	2 cut(s)	1368, 1627
BspLI	GGNNCC	7 cut(s)	134, 135, 384, 433, 516, 906, 1433
BspPI	GGATC	3 cut(s)	576, 790, 1193
BspT104I	TTCGAA	1 cut(s)	1470
BspT107I	GGYRCC	1 cut(s)	904
BspTNI	GGTCTC	2 cut(s)	988, 1362
BsrDI	GCAATG	2 cut(s)	214, 448
BsrFI	RCCGGY	1 cut(s)	248
BsrI	ACTGG	7 cut(s)	69, 121, 475, 570, 1079, 1509, 1559
BssAI	RCCGGY	1 cut(s)	248
BssECI	CCNNGG	3 cut(s)	1210, 1429, 1691
BssMI	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
BssNI	GRCGYC	1 cut(s)	905
BssSI	CACGAG	3 cut(s)	540, 749, 1713
BssT1I	CCWWGG	1 cut(s)	1691
Bst2BI	CACGAG	3 cut(s)	540, 749, 1713
Bst2UI	CCWGG	4 cut(s)	42, 1008, 1482, 1520
Bst4CI	ACNGT	5 cut(s)	123, 746, 918, 1211, 1456
BstACI	GRCGYC	1 cut(s)	905
BstAPI	GCANNNNNTGC	1 cut(s)	724
BstBI	TTCGAA	1 cut(s)	1470
BstC8I	GCNNGC	5 cut(s)	376, 737, 837, 1370, 1629
BstDEI	CTNAG	6 cut(s)	519, 861, 909, 1261, 1395, 1584
BstDSI	CCRYGG	1 cut(s)	1210
BstF5I	GGATG	7 cut(s)	235, 316, 331, 335, 571, 1309, 1661
BstFNI	CGCG	2 cut(s)	1368, 1627
BstH2I	RGCGCY	3 cut(s)	868, 908, 1375
BstHHI	GCGC	4 cut(s)	867, 907, 1374, 1627
BstKTI	GATC	6 cut(s)	113, 571, 785, 952, 1201, 1545
BstMAI	GTCTC	5 cut(s)	453, 765, 988, 1362, 1372
BstMBI	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
BstMWI	GCNNNNNNNGC	9 cut(s)	55, 151, 198, 204, 224, 340, 724, 864, 1226
BstNI	CCWGG	4 cut(s)	42, 1008, 1482, 1520
BstNSI	RCATGY	2 cut(s)	739, 1269
BstSCI	CCNGG	5 cut(s)	40, 1006, 1428, 1480, 1518
BstSFI	CTRYAG	3 cut(s)	436, 927, 1436
BstSLI	GKGCMC	1 cut(s)	78
BstUI	CGCG	2 cut(s)	1368, 1627
BstV1I	GCAGC	5 cut(s)	160, 163, 213, 216, 751
BstX2I	RGATCY	1 cut(s)	1198
BstYI	RGATCY	1 cut(s)	1198
BsuRI	GGCC	6 cut(s)	17, 91, 167, 334, 1434, 1527
BtgI	CCRYGG	1 cut(s)	1210
BtrI	CACGTC	1 cut(s)	79
BtsCI	GGATG	7 cut(s)	235, 316, 331, 335, 571, 1309, 1661
BtsI	GCAGTG	1 cut(s)	199
BtsIMutI	CAGTG	5 cut(s)	199, 345, 468, 742, 1516
Cac8I	GCNNGC	5 cut(s)	376, 737, 837, 1370, 1629
CfoI	GCGC	4 cut(s)	867, 907, 1374, 1627
Cfr10I	RCCGGY	1 cut(s)	248
Cfr13I	GGNCC	5 cut(s)	89, 133, 333, 496, 1432
CsiI	ACCWGGT	1 cut(s)	40
Csp6I	GTAC	3 cut(s)	619, 898, 1120
CspAI	ACCGGT	1 cut(s)	248
CviAII	CATG	4 cut(s)	188, 646, 736, 1266
CviQI	GTAC	3 cut(s)	619, 898, 1120
DdeI	CTNAG	6 cut(s)	519, 861, 909, 1261, 1395, 1584
DinI	GGCGCC	1 cut(s)	906
DpnI	GATC	6 cut(s)	112, 570, 784, 951, 1200, 1544
DpnII	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
DraIII	CACNNNGTG	1 cut(s)	44
EciI	GGCGGA	1 cut(s)	242
Eco130I	CCWWGG	1 cut(s)	1691
Eco31I	GGTCTC	2 cut(s)	988, 1362
Eco47I	GGWCC	2 cut(s)	133, 496
Eco47III	AGCGCT	1 cut(s)	866
Eco57I	CTGAAG	2 cut(s)	702, 1106
Eco88I	CYCGRG	1 cut(s)	456
EcoO109I	RGGNCCY	2 cut(s)	133, 1432
EcoRI	GAATTC	2 cut(s)	1241, 1673
EcoRII	CCWGG	4 cut(s)	40, 1006, 1480, 1518
EcoT14I	CCWWGG	1 cut(s)	1691
EcoT22I	ATGCAT	1 cut(s)	720
EgeI	GGCGCC	1 cut(s)	906
EheI	GGCGCC	1 cut(s)	906
ErhI	CCWWGG	1 cut(s)	1691
FaeI	CATG	4 cut(s)	191, 649, 739, 1269
FalI	AAGNNNNNCTT	2 cut(s)	78, 110
FaqI	GGGAC	2 cut(s)	146, 479
FatI	CATG	4 cut(s)	187, 645, 735, 1265
FblI	GTMKAC	1 cut(s)	1282
FokI	GGATG	7 cut(s)	242, 323, 338, 342, 558, 1316, 1648
FspBI	CTAG	5 cut(s)	50, 1319, 1341, 1700, 1723
GlaI	GCGC	4 cut(s)	866, 906, 1373, 1626
GsuI	CTGGAG	1 cut(s)	1492
HaeII	RGCGCY	3 cut(s)	868, 908, 1375
HaeIII	GGCC	6 cut(s)	17, 91, 167, 334, 1434, 1527
HapII	CCGG	4 cut(s)	249, 499, 1429, 1528
HhaI	GCGC	4 cut(s)	867, 907, 1374, 1627
Hin1I	GRCGYC	1 cut(s)	905
Hin1II	CATG	4 cut(s)	191, 649, 739, 1269
Hin6I	GCGC	4 cut(s)	865, 905, 1372, 1625
HinP1I	GCGC	4 cut(s)	865, 905, 1372, 1625
HincII	GTYRAC	1 cut(s)	246
HindII	GTYRAC	1 cut(s)	246
HindIII	AAGCTT	2 cut(s)	587, 1140
HinfI	GANTC	3 cut(s)	280, 398, 1460
HpaII	CCGG	4 cut(s)	249, 499, 1429, 1528
HphI	GGTGA	4 cut(s)	19, 173, 437, 1487
Hpy166II	GTNNAC	4 cut(s)	246, 987, 1271, 1283
Hpy188I	TCNGA	8 cut(s)	285, 514, 655, 1066, 1233, 1253, 1647, 1679
Hpy188III	TCNNGA	8 cut(s)	458, 609, 749, 770, 1136, 1359, 1594, 1715
Hpy8I	GTNNAC	4 cut(s)	246, 987, 1271, 1283
Hpy99I	CGWCG	2 cut(s)	83, 223
HpyAV	CCTTC	9 cut(s)	422, 487, 587, 677, 1466, 1517, 1525, 1559, 1674
HpyCH4III	ACNGT	5 cut(s)	123, 746, 918, 1211, 1456
HpyCH4IV	ACGT	2 cut(s)	78, 1114
HpyF10VI	GCNNNNNNNGC	9 cut(s)	55, 151, 198, 204, 224, 340, 724, 864, 1226
HpyF3I	CTNAG	6 cut(s)	519, 861, 909, 1261, 1395, 1584
HpySE526I	ACGT	2 cut(s)	78, 1114
Hsp92I	GRCGYC	1 cut(s)	905
Hsp92II	CATG	4 cut(s)	191, 649, 739, 1269
HspAI	GCGC	4 cut(s)	865, 905, 1372, 1625
KasI	GGCGCC	1 cut(s)	904
KflI	GGGWCCC	1 cut(s)	133
Kzo9I	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
LmnI	GCTCC	3 cut(s)	38, 1441, 1696
Lsp1109I	GCAGC	5 cut(s)	160, 163, 213, 216, 751
LweI	GCATC	3 cut(s)	727, 1427, 1670
MabI	ACCWGGT	1 cut(s)	40
MaeI	CTAG	5 cut(s)	50, 1319, 1341, 1700, 1723
MaeII	ACGT	2 cut(s)	78, 1114
MaeIII	GTNAC	4 cut(s)	101, 746, 886, 1579
MalI	GATC	6 cut(s)	112, 570, 784, 951, 1200, 1544
MboI	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
MboII	GAAGA	5 cut(s)	502, 1124, 1155, 1413, 1537
MfeI	CAATTG	1 cut(s)	722
MflI	RGATCY	1 cut(s)	1198
MhlI	GDGCHC	2 cut(s)	78, 1184
Mly113I	GGCGCC	1 cut(s)	905
MlyI	GAGTC	2 cut(s)	289, 392
MmeI	TCCRAC	2 cut(s)	1449, 1689
Mph1103I	ATGCAT	1 cut(s)	720
MroXI	GAANNNNTTC	1 cut(s)	670
MseI	TTAA	5 cut(s)	96, 141, 528, 1403, 1650
MslI	CAYNNNNRTG	2 cut(s)	192, 576
MspI	CCGG	4 cut(s)	249, 499, 1429, 1528
MspR9I	CCNGG	5 cut(s)	42, 1008, 1430, 1482, 1520
MunI	CAATTG	1 cut(s)	722
Mva1269I	GAATGC	2 cut(s)	618, 816
MvaI	CCWGG	4 cut(s)	42, 1008, 1482, 1520
MvnI	CGCG	2 cut(s)	1368, 1627
MwoI	GCNNNNNNNGC	9 cut(s)	55, 151, 198, 204, 224, 340, 724, 864, 1226
NarI	GGCGCC	1 cut(s)	905
NciI	CCSGG	1 cut(s)	1430
NdeII	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
NlaIII	CATG	4 cut(s)	191, 649, 739, 1269
NlaIV	GGNNCC	7 cut(s)	134, 135, 384, 433, 516, 906, 1433
NmuCI	GTSAC	3 cut(s)	101, 746, 886
NsiI	ATGCAT	1 cut(s)	720
NspI	RCATGY	2 cut(s)	739, 1269
NspV	TTCGAA	1 cut(s)	1470
PaeI	GCATGC	1 cut(s)	739
PaeR7I	CTCGAG	1 cut(s)	456
PcsI	WCGNNNNNNNCGW	1 cut(s)	411
PctI	GAATGC	2 cut(s)	618, 816
PdmI	GAANNNNTTC	1 cut(s)	670
PfeI	GAWTC	1 cut(s)	1460
PinAI	ACCGGT	1 cut(s)	248
PleI	GAGTC	2 cut(s)	288, 392
PluTI	GGCGCC	1 cut(s)	908
PpsI	GAGTC	2 cut(s)	288, 392
PpuMI	RGGWCCY	1 cut(s)	133
PsiI	TTATAA	1 cut(s)	297
Psp5II	RGGWCCY	1 cut(s)	133
Psp6I	CCWGG	4 cut(s)	40, 1006, 1480, 1518
PspGI	CCWGG	4 cut(s)	40, 1006, 1480, 1518
PspN4I	GGNNCC	7 cut(s)	134, 135, 384, 433, 516, 906, 1433
PspPI	GGNCC	5 cut(s)	89, 133, 333, 496, 1432
PspPPI	RGGWCCY	1 cut(s)	133
PsuI	RGATCY	1 cut(s)	1198
RsaI	GTAC	3 cut(s)	620, 899, 1121
RsaNI	GTAC	3 cut(s)	619, 898, 1120
RseI	CAYNNNNRTG	2 cut(s)	192, 576
SaqAI	TTAA	5 cut(s)	96, 141, 528, 1403, 1650
Sau3AI	GATC	6 cut(s)	110, 568, 782, 949, 1198, 1542
Sau96I	GGNCC	5 cut(s)	89, 133, 333, 496, 1432
SchI	GAGTC	2 cut(s)	289, 392
ScrFI	CCNGG	5 cut(s)	42, 1008, 1430, 1482, 1520
SduI	GDGCHC	2 cut(s)	78, 1184
SexAI	ACCWGGT	1 cut(s)	40
SfaNI	GCATC	3 cut(s)	727, 1427, 1670
SfcI	CTRYAG	3 cut(s)	436, 927, 1436
SfoI	GGCGCC	1 cut(s)	906
Sfr274I	CTCGAG	1 cut(s)	456
SfuI	TTCGAA	1 cut(s)	1470
SinI	GGWCC	2 cut(s)	133, 496
SlaI	CTCGAG	1 cut(s)	456
SmiMI	CAYNNNNRTG	2 cut(s)	192, 576
SmlI	CTYRAG	2 cut(s)	456, 607
SmoI	CTYRAG	2 cut(s)	456, 607
SphI	GCATGC	1 cut(s)	739
SsiI	CCGC	9 cut(s)	36, 92, 146, 198, 224, 227, 239, 1301, 1354
SspDI	GGCGCC	1 cut(s)	904
SspI	AATATT	1 cut(s)	355
SspMI	CTAG	5 cut(s)	50, 1319, 1341, 1700, 1723
StyD4I	CCNGG	5 cut(s)	40, 1006, 1428, 1480, 1518
StyI	CCWWGG	1 cut(s)	1691
TaaI	ACNGT	5 cut(s)	123, 746, 918, 1211, 1456
TaiI	ACGT	2 cut(s)	81, 1117
TaqI	TCGA	8 cut(s)	183, 359, 414, 457, 769, 1360, 1470, 1563
TatI	WGTACW	1 cut(s)	618
TauI	GCSGC	5 cut(s)	94, 148, 201, 227, 242
TfiI	GAWTC	1 cut(s)	1460
Tru1I	TTAA	5 cut(s)	96, 141, 528, 1403, 1650
Tru9I	TTAA	5 cut(s)	96, 141, 528, 1403, 1650
TscAI	CASTG	5 cut(s)	199, 352, 475, 749, 1516
TseFI	GTSAC	3 cut(s)	101, 746, 886
TseI	GCWGC	5 cut(s)	148, 151, 201, 204, 739
Tsp45I	GTSAC	3 cut(s)	101, 746, 886
TspDTI	ATGAA	5 cut(s)	225, 634, 1022, 1155, 1323
TspGWI	ACGGA	1 cut(s)	574
TspRI	CASTG	5 cut(s)	199, 352, 475, 749, 1516
VpaK11BI	GGWCC	2 cut(s)	133, 496
XapI	RAATTY	5 cut(s)	530, 1241, 1410, 1465, 1673
XceI	RCATGY	2 cut(s)	739, 1269
XhoI	CTCGAG	1 cut(s)	456
XmiI	GTMKAC	1 cut(s)	1282
XmnI	GAANNNNTTC	1 cut(s)	670
XspI	CTAG	5 cut(s)	50, 1319, 1341, 1700, 1723
Zsp2I	ATGCAT	1 cut(s)	720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.