Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010524

Physical Location & Seq

Genomic Coordinates Forward (+)

35830324 .. 35832695

Gene Structure 2372 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ24611

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGCTTCGCCACATCTATCGATCTTCTTCCTTCTCTTCATCATCCCACAGAAAACCCAAACCCAAACCAAAACCGCTCCCCATGGGCTGCGTCCAAGCCAAACCCGAGATGAACGGTTCCACCAGACCTGTGGGGCTCGAAAGGCTCAAGCTGGATAATGGTTATGTGGGGAATGCTCAGAGAAGACCACCGCCGAGTGTGGATGATGGCAGTGGTAACGGCACTGGAGGAGAGGATCATAGTGAGGTGGCCGAGAGAGATAATAATAATAATAATAATAAGCATCCTCCTAGTTCTCGTAGAATTTCTGCTGCTGATGACGACGAGTTGGTCGATGGCTGGCCTAAATGGCTCACTGATAATATTTCCAAAGACGTTTTGGCTGGCTTGGTTCCCAAGAGTGCCGACTCCTATGATAAACTCGACAAGGTGGGCGAAGGAACCTACAGCAATGTTTACAAGGCTCGAGACAGGGACACTGGAAAAATTGTTGCCTTGAAGAAGGTCCGTTTTGATACATCAGAACCTGAGAGTGTTAAATTTATGGCAAGAGAGATTATGGTATTAAGGAAATTGGATCATCCCAATGTGCTTAAGCTTGAAGGATTGGCCACTTCAAGAATGCAATATAGCATTTATCTTGTCTTTGATTTCATGCACTCAGATTTGGCAAAAGTTATTTCCAAAGCCGCAGGGCTTACTGAACCACAGGTCAAGTGCTATATGCATCAACTGCTTTCAGGTCTGCAGCACTGCCATGATCGTGGAATTTTGCATCGAGACATTAAAGGGTCGAACTTGTTGATGGATAAAAATGGGGTTCTAAAAATTGGCGATTTTGGGCTTGCGAATTATTATTCCCCAAAGCCCAAACATCCTCTTACCAACCGAGTTGTGACACTTTGGTACAGAGCTCCCGAGCTGCTGTTGGGTTCTACAGATTATGGAGTTGGAATTGATCTTTGGAGCGCTGGATGCGTCATGGCTGAAATGTTTACAGGGAGGCCACTTATGCCTGGCAGAACAGAGGTTGAGCAACTTCATAGGATTTTTAAGCTTTGTGGCACACCATCTGAGGAATACTGGAAAAAACTGAAGCTTTCTACAACTTTTCGACCTCCACGTTCATACAAACCCAGTCTTCAGGAAGCTCTTAAGGATTTACCTTCATCTTCCTTGGGTCTGTTGGGCACTCTTCTTGCTTTAGATCCTGCCTATCGAGGCAGTGCAGCTCTAGGTCTCAAAAATGAAGTACGTAGAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

421

Amino Acids

46.91

Weight (kDa)

9.42

Isoelectric Point (pI)

36.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	138 - 411	4e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	142 - 350	6.2e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010524)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090 FvH4_2g13090
malus_domestica	MD05G1032700.v1.1 MD10G1033600.v1.1
prunus_persica	Prupe.8G039300_v2.0.a1 Prupe.8G039300_v2.0.a1 Prupe.8G039300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0274351
rosa_laevigata	RLG00000013550
rosa_multiflora	Rmu_ssc0000361.1_g000017
rosa_roxburghii	Rroxscaffold_7G00194310
rosa_rugosa	Rorug06G0081100
rosa_samantha	Rh6AG196300 Rh6BG200500 Rh6CG201400 Rh6DG192200
rosa_wichuraiana	Rw6G017150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	329
AccBSI	CCGCTC	1 cut(s)	76
AciI	CCGC	3 cut(s)	74, 191, 690
AclWI	GGATC	3 cut(s)	243, 585, 1202
AcoI	YGGCCR	2 cut(s)	249, 609
AcsI	RAATTY	3 cut(s)	303, 539, 768
AcuI	CTGAAG	2 cut(s)	1115, 1127
AfaI	GTAC	2 cut(s)	908, 1254
AfeI	AGCGCT	1 cut(s)	970
AflII	CTTAAG	2 cut(s)	593, 1154
AgsI	TTSAA	3 cut(s)	499, 602, 618
AjnI	CCWGG	1 cut(s)	1015
AjuI	GAANNNNNNNTTGG	2 cut(s)	946, 978
AluBI	AGCT	8 cut(s)	151, 598, 914, 922, 1057, 1099, 1151, 1232
AluI	AGCT	8 cut(s)	151, 598, 914, 922, 1057, 1099, 1151, 1232
Alw21I	GWGCWC	1 cut(s)	916
Alw26I	GTCTC	3 cut(s)	462, 774, 1244
AlwI	GGATC	3 cut(s)	243, 585, 1202
AlwNI	CAGNNNCTG	1 cut(s)	527
Ama87I	CYCGRG	3 cut(s)	104, 465, 917
Aor51HI	AGCGCT	1 cut(s)	970
AoxI	GGCC	4 cut(s)	249, 341, 609, 1004
ApeKI	GCWGC	5 cut(s)	87, 311, 748, 922, 1229
ApoI	RAATTY	3 cut(s)	303, 539, 768
AspLEI	GCGC	1 cut(s)	971
AspS9I	GGNCC	1 cut(s)	505
AvaI	CYCGRG	3 cut(s)	104, 465, 917
AvaII	GGWCC	1 cut(s)	505
BaeGI	GKGCMC	1 cut(s)	1193
BalI	TGGCCA	1 cut(s)	611
BanII	GRGCYC	2 cut(s)	138, 916
BbsI	GAAGAC	2 cut(s)	190, 1133
Bbv12I	GWGCWC	1 cut(s)	916
BbvI	GCAGC	5 cut(s)	74, 298, 760, 909, 1241
BccI	CCATC	4 cut(s)	200, 329, 799, 1078
BceAI	ACGGC	1 cut(s)	235
BciT130I	CCWGG	1 cut(s)	1017
BcoDI	GTCTC	3 cut(s)	462, 774, 1244
BfaI	CTAG	2 cut(s)	291, 1235
BfmI	CTRYAG	3 cut(s)	445, 746, 936
BfoI	RGCGCY	1 cut(s)	972
BfrI	CTTAAG	2 cut(s)	593, 1154
BglI	GCCNNNNNGGC	1 cut(s)	349
BisI	GCNGC	6 cut(s)	88, 312, 690, 749, 923, 1230
BlsI	GCNGC	6 cut(s)	89, 313, 691, 750, 924, 1231
Bme1390I	CCNGG	1 cut(s)	1017
Bme18I	GGWCC	1 cut(s)	505
BmeT110I	CYCGRG	3 cut(s)	104, 465, 917
BmgT120I	GGNCC	1 cut(s)	505
BmiI	GGNNCC	3 cut(s)	118, 393, 442
BmrFI	CCNGG	1 cut(s)	1017
BmrI	ACTGGG	1 cut(s)	1131
BmsI	GCATC	4 cut(s)	292, 736, 784, 965
BmuI	ACTGGG	1 cut(s)	1131
BpiI	GAAGAC	2 cut(s)	190, 1133
BpmI	CTGGAG	1 cut(s)	246
BpuEI	CTTGAG	1 cut(s)	131
Bsa29I	ATCGAT	1 cut(s)	19
BsaAI	YACGTR	1 cut(s)	1256
BsaI	GGTCTC	1 cut(s)	1244
BsaJI	CCNNGG	2 cut(s)	81, 1176
Bse1I	ACTGG	4 cut(s)	229, 484, 1088, 1137
Bse3DI	GCAATG	1 cut(s)	457
BseBI	CCWGG	1 cut(s)	1017
BseCI	ATCGAT	1 cut(s)	19
BseDI	CCNNGG	2 cut(s)	81, 1176
BseGI	GGATG	6 cut(s)	41, 208, 283, 580, 874, 980
BseMI	GCAATG	1 cut(s)	457
BseMII	CTCAG	4 cut(s)	191, 519, 675, 1065
BseNI	ACTGG	4 cut(s)	229, 484, 1088, 1137
BseRI	GAGGAG	1 cut(s)	243
BseSI	GKGCMC	1 cut(s)	1193
BseXI	GCAGC	5 cut(s)	74, 298, 760, 909, 1241
BsgI	GTGCAG	1 cut(s)	1248
BshFI	GGCC	4 cut(s)	251, 343, 611, 1006
BshVI	ATCGAT	1 cut(s)	19
BsiHKAI	GWGCWC	1 cut(s)	916
BsiHKCI	CYCGRG	3 cut(s)	104, 465, 917
BslFI	GGGAC	1 cut(s)	488
BsmAI	GTCTC	3 cut(s)	462, 774, 1244
BsmFI	GGGAC	1 cut(s)	488
BsmI	GAATGC	2 cut(s)	178, 627
BsnI	GGCC	4 cut(s)	251, 343, 611, 1006
Bso31I	GGTCTC	1 cut(s)	1244
BsoBI	CYCGRG	3 cut(s)	104, 465, 917
Bsp1286I	GDGCHC	3 cut(s)	138, 916, 1193
Bsp143I	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
Bsp19I	CCATGG	1 cut(s)	81
BspACI	CCGC	3 cut(s)	74, 191, 690
BspANI	GGCC	4 cut(s)	251, 343, 611, 1006
BspCNI	CTCAG	4 cut(s)	190, 520, 674, 1066
BspDI	ATCGAT	1 cut(s)	19
BspLI	GGNNCC	3 cut(s)	118, 393, 442
BspMAI	CTGCAG	1 cut(s)	750
BspPI	GGATC	3 cut(s)	243, 585, 1202
BspTI	CTTAAG	2 cut(s)	593, 1154
BspTNI	GGTCTC	1 cut(s)	1244
BsrBI	CCGCTC	1 cut(s)	76
BsrDI	GCAATG	1 cut(s)	457
BsrI	ACTGG	4 cut(s)	229, 484, 1088, 1137
BssECI	CCNNGG	2 cut(s)	81, 1176
BssMI	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
BssT1I	CCWWGG	2 cut(s)	81, 1176
Bst2UI	CCWGG	1 cut(s)	1017
Bst4CI	ACNGT	1 cut(s)	116
Bst6I	CTCTTC	2 cut(s)	40, 1200
BstAFI	CTTAAG	2 cut(s)	593, 1154
BstAPI	GCANNNNNTGC	1 cut(s)	733
BstBAI	YACGTR	1 cut(s)	1256
BstC8I	GCNNGC	3 cut(s)	341, 385, 846
BstDEI	CTNAG	4 cut(s)	177, 528, 661, 1074
BstDSI	CCRYGG	1 cut(s)	81
BstF5I	GGATG	6 cut(s)	41, 208, 283, 580, 874, 980
BstH2I	RGCGCY	1 cut(s)	972
BstHHI	GCGC	1 cut(s)	971
BstKTI	GATC	6 cut(s)	23, 238, 580, 763, 961, 1210
BstMAI	GTCTC	3 cut(s)	462, 774, 1244
BstMBI	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
BstMWI	GCNNNNNNNGC	5 cut(s)	142, 349, 733, 975, 1012
BstNI	CCWGG	1 cut(s)	1017
BstSCI	CCNGG	1 cut(s)	1015
BstSFI	CTRYAG	3 cut(s)	445, 746, 936
BstSLI	GKGCMC	1 cut(s)	1193
BstSNI	TACGTA	1 cut(s)	1256
BstV1I	GCAGC	5 cut(s)	74, 298, 760, 909, 1241
BstV2I	GAAGAC	2 cut(s)	190, 1133
BstX2I	RGATCY	1 cut(s)	1207
BstXI	CCANNNNNNTGG	2 cut(s)	130, 764
BstYI	RGATCY	1 cut(s)	1207
Bsu15I	ATCGAT	1 cut(s)	19
BsuRI	GGCC	4 cut(s)	251, 343, 611, 1006
BsuTUI	ATCGAT	1 cut(s)	19
BtgI	CCRYGG	1 cut(s)	81
BtsCI	GGATG	6 cut(s)	41, 208, 283, 580, 874, 980
BtsI	GCAGTG	3 cut(s)	217, 751, 1231
BtsIMutI	CAGTG	6 cut(s)	217, 222, 354, 477, 751, 1231
Cac8I	GCNNGC	3 cut(s)	341, 385, 846
CaiI	CAGNNNCTG	1 cut(s)	527
CfoI	GCGC	1 cut(s)	971
Cfr13I	GGNCC	1 cut(s)	505
ClaI	ATCGAT	1 cut(s)	19
CseI	GACGC	2 cut(s)	79, 967
Csp6I	GTAC	2 cut(s)	907, 1253
CviAII	CATG	4 cut(s)	82, 655, 758, 982
CviQI	GTAC	2 cut(s)	907, 1253
DdeI	CTNAG	4 cut(s)	177, 528, 661, 1074
DpnI	GATC	6 cut(s)	22, 237, 579, 762, 960, 1209
DpnII	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
DrdI	GACNNNNNNGTC	1 cut(s)	329
DseDI	GACNNNNNNGTC	1 cut(s)	329
EaeI	YGGCCR	2 cut(s)	249, 609
Eam1104I	CTCTTC	2 cut(s)	40, 1200
EarI	CTCTTC	2 cut(s)	40, 1200
Ecl136II	GAGCTC	1 cut(s)	914
Eco105I	TACGTA	1 cut(s)	1256
Eco130I	CCWWGG	2 cut(s)	81, 1176
Eco24I	GRGCYC	2 cut(s)	138, 916
Eco31I	GGTCTC	1 cut(s)	1244
Eco47I	GGWCC	1 cut(s)	505
Eco47III	AGCGCT	1 cut(s)	970
Eco53kI	GAGCTC	1 cut(s)	914
Eco57I	CTGAAG	2 cut(s)	1115, 1127
Eco88I	CYCGRG	3 cut(s)	104, 465, 917
EcoICRI	GAGCTC	1 cut(s)	914
EcoRII	CCWGG	1 cut(s)	1015
EcoT14I	CCWWGG	2 cut(s)	81, 1176
EcoT22I	ATGCAT	1 cut(s)	729
EcoT38I	GRGCYC	2 cut(s)	138, 916
ErhI	CCWWGG	2 cut(s)	81, 1176
FaeI	CATG	4 cut(s)	85, 658, 761, 985
FaqI	GGGAC	1 cut(s)	488
FatI	CATG	4 cut(s)	81, 654, 757, 981
Fnu4HI	GCNGC	6 cut(s)	88, 312, 690, 749, 923, 1230
FokI	GGATG	6 cut(s)	28, 215, 270, 567, 861, 987
FriOI	GRGCYC	2 cut(s)	138, 916
Fsp4HI	GCNGC	6 cut(s)	88, 312, 690, 749, 923, 1230
FspBI	CTAG	2 cut(s)	291, 1235
GlaI	GCGC	1 cut(s)	970
GluI	GCNGC	6 cut(s)	88, 312, 690, 749, 923, 1230
GsuI	CTGGAG	1 cut(s)	246
HaeII	RGCGCY	1 cut(s)	972
HaeIII	GGCC	4 cut(s)	251, 343, 611, 1006
HgaI	GACGC	2 cut(s)	79, 967
HhaI	GCGC	1 cut(s)	971
Hin1II	CATG	4 cut(s)	85, 658, 761, 985
Hin6I	GCGC	1 cut(s)	969
HinP1I	GCGC	1 cut(s)	969
HindIII	AAGCTT	3 cut(s)	596, 1055, 1097
HinfI	GANTC	1 cut(s)	407
Hpy166II	GTNNAC	2 cut(s)	457, 996
Hpy188I	TCNGA	4 cut(s)	180, 523, 664, 1075
Hpy188III	TCNNGA	5 cut(s)	467, 618, 779, 917, 1145
Hpy8I	GTNNAC	2 cut(s)	457, 996
Hpy99I	CGWCG	1 cut(s)	326
HpyAV	CCTTC	5 cut(s)	40, 431, 496, 596, 1176
HpyCH4III	ACNGT	1 cut(s)	116
HpyCH4IV	ACGT	3 cut(s)	375, 1123, 1255
HpyCH4V	TGCA	6 cut(s)	625, 658, 727, 748, 775, 1229
HpyF10VI	GCNNNNNNNGC	5 cut(s)	142, 349, 733, 975, 1012
HpyF3I	CTNAG	4 cut(s)	177, 528, 661, 1074
HpySE526I	ACGT	3 cut(s)	375, 1123, 1255
Hsp92II	CATG	4 cut(s)	85, 658, 761, 985
HspAI	GCGC	1 cut(s)	969
Kzo9I	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
LmnI	GCTCC	3 cut(s)	81, 919, 966
Lsp1109I	GCAGC	5 cut(s)	74, 298, 760, 909, 1241
LweI	GCATC	4 cut(s)	292, 736, 784, 965
MaeI	CTAG	2 cut(s)	291, 1235
MaeII	ACGT	3 cut(s)	375, 1123, 1255
MaeIII	GTNAC	2 cut(s)	215, 895
MalI	GATC	6 cut(s)	22, 237, 579, 762, 960, 1209
MbiI	CCGCTC	1 cut(s)	76
MboI	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
MboII	GAAGA	8 cut(s)	15, 18, 27, 195, 511, 1133, 1164, 1187
MflI	RGATCY	1 cut(s)	1207
MhlI	GDGCHC	3 cut(s)	138, 916, 1193
MlsI	TGGCCA	1 cut(s)	611
MluCI	AATT	8 cut(s)	303, 486, 539, 572, 768, 828, 850, 954
MluNI	TGGCCA	1 cut(s)	611
MlyI	GAGTC	1 cut(s)	401
MmeI	TCCRAC	1 cut(s)	931
Mox20I	TGGCCA	1 cut(s)	611
Mph1103I	ATGCAT	1 cut(s)	729
MscI	TGGCCA	1 cut(s)	611
MseI	TTAA	6 cut(s)	537, 566, 594, 786, 1053, 1155
MslI	CAYNNNNRTG	3 cut(s)	585, 756, 762
Msp20I	TGGCCA	1 cut(s)	611
MspCI	CTTAAG	2 cut(s)	593, 1154
MspR9I	CCNGG	1 cut(s)	1017
Mva1269I	GAATGC	2 cut(s)	178, 627
MvaI	CCWGG	1 cut(s)	1017
MwoI	GCNNNNNNNGC	5 cut(s)	142, 349, 733, 975, 1012
NcoI	CCATGG	1 cut(s)	81
NdeII	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
NlaIII	CATG	4 cut(s)	85, 658, 761, 985
NlaIV	GGNNCC	3 cut(s)	118, 393, 442
NmeAIII	GCCGAG	2 cut(s)	219, 277
NmuCI	GTSAC	1 cut(s)	895
NsiI	ATGCAT	1 cut(s)	729
PaeR7I	CTCGAG	1 cut(s)	465
PcsI	WCGNNNNNNNCGW	2 cut(s)	330, 1120
PctI	GAATGC	2 cut(s)	178, 627
PkrI	GCNGC	6 cut(s)	89, 313, 691, 750, 924, 1231
PleI	GAGTC	1 cut(s)	401
PpsI	GAGTC	1 cut(s)	401
Ppu21I	YACGTR	1 cut(s)	1256
Psp124BI	GAGCTC	1 cut(s)	916
Psp6I	CCWGG	1 cut(s)	1015
PspGI	CCWGG	1 cut(s)	1015
PspN4I	GGNNCC	3 cut(s)	118, 393, 442
PspPI	GGNCC	1 cut(s)	505
PstI	CTGCAG	1 cut(s)	750
PstNI	CAGNNNCTG	1 cut(s)	527
PsuI	RGATCY	1 cut(s)	1207
RsaI	GTAC	2 cut(s)	908, 1254
RsaNI	GTAC	2 cut(s)	907, 1253
RseI	CAYNNNNRTG	3 cut(s)	585, 756, 762
SacI	GAGCTC	1 cut(s)	916
SaqAI	TTAA	6 cut(s)	537, 566, 594, 786, 1053, 1155
SatI	GCNGC	6 cut(s)	88, 312, 690, 749, 923, 1230
Sau3AI	GATC	6 cut(s)	20, 235, 577, 760, 958, 1207
Sau96I	GGNCC	1 cut(s)	505
SchI	GAGTC	1 cut(s)	401
ScrFI	CCNGG	1 cut(s)	1017
SduI	GDGCHC	3 cut(s)	138, 916, 1193
SfaNI	GCATC	4 cut(s)	292, 736, 784, 965
SfcI	CTRYAG	3 cut(s)	445, 746, 936
Sfr274I	CTCGAG	1 cut(s)	465
SinI	GGWCC	1 cut(s)	505
SlaI	CTCGAG	1 cut(s)	465
SmiMI	CAYNNNNRTG	3 cut(s)	585, 756, 762
SmlI	CTYRAG	4 cut(s)	146, 465, 593, 1154
SmoI	CTYRAG	4 cut(s)	146, 465, 593, 1154
SnaBI	TACGTA	1 cut(s)	1256
Sse9I	AATT	8 cut(s)	303, 486, 539, 572, 768, 828, 850, 954
SsiI	CCGC	3 cut(s)	74, 191, 690
SspI	AATATT	1 cut(s)	364
SspMI	CTAG	2 cut(s)	291, 1235
SstI	GAGCTC	1 cut(s)	916
StyD4I	CCNGG	1 cut(s)	1015
StyI	CCWWGG	2 cut(s)	81, 1176
TaaI	ACNGT	1 cut(s)	116
TaiI	ACGT	3 cut(s)	378, 1126, 1258
TaqI	TCGA	9 cut(s)	19, 138, 333, 423, 466, 778, 794, 1114, 1219
TasI	AATT	8 cut(s)	303, 486, 539, 572, 768, 828, 850, 954
TauI	GCSGC	1 cut(s)	692
Tru1I	TTAA	6 cut(s)	537, 566, 594, 786, 1053, 1155
Tru9I	TTAA	6 cut(s)	537, 566, 594, 786, 1053, 1155
TscAI	CASTG	6 cut(s)	217, 229, 361, 484, 758, 1231
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	5 cut(s)	87, 311, 748, 922, 1229
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	7 cut(s)	27, 125, 643, 1031, 1116, 1158, 1263
TspGWI	ACGGA	1 cut(s)	497
TspRI	CASTG	6 cut(s)	217, 229, 361, 484, 758, 1231
Vha464I	CTTAAG	2 cut(s)	593, 1154
VpaK11BI	GGWCC	1 cut(s)	505
XapI	RAATTY	3 cut(s)	303, 539, 768
XcmI	CCANNNNNNNNNTGG	2 cut(s)	127, 376
XhoI	CTCGAG	1 cut(s)	465
XspI	CTAG	2 cut(s)	291, 1235
Zsp2I	ATGCAT	1 cut(s)	729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.