MD05G1045400.v1.1

Endoplasmic reticulum-Golgi intermediate compartment protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0020163
Physical Location & Seq
Reverse (-)
7763194 .. 7771372
8179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1045400.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGGGCAGGGGCGGTGGTATCCTTGAGAAGCTGAGGAGTTTGGACGCCTACCCTAAAATCAATGACGATTTCTTCAGCCGTACACTCTCCGGTGGCATCATTACCCTCGCCTCTTCCCTCCTCATGCTCTTCCTTTTCTTCTCCGAGCTCAGACTATACCTCCACACTGTTACAGAGTCAAAGCTTCTTGTAGATGCTTCAAGAGGAGAAACCCTACATATCAATTTTGATGTAACTTTTCCTGCTATCCGATGCTCATTACTCAGTCTTGACACAATGGATATTAGCGGAGAGCAGCATTTTGACATACGACACCATATAGTCAAGAAACGAATTGATGCGCATGGTAATGTAATAGAAGCTAAGAAGGATGGAATTGGTGCACCAAAGATTGAGAAACCCTTGCAAAGGCATGGTGGAAGGCTTGAACATAATGAAAAATATTGTGGATCGTGCTTTGGAGCAGAAACGTCTGATGATGATTGTTGCAACTCGTGCGATGAAGTTCGTGATGCATATAGGAAAAAAGGTTGGGCACTGACGAATGCAGAGTTGATAGACCAGTGCGAACGGGAAGGTTTTATTCAAAAGGTCAAAGATGAAGATGGTGAAGGATGTAATATTGAAGGATCCCTTGAAGTTAACAAAGTGGCTGGAAATTTTCATTTTATACCTGGAAAGAGTTTTCACCAGTCTAATGTTCATTTGCATGATTTGCTGGCTTTCCCGACAGATAGTTACAATATAAGTCACAAGATAAACAGATTAGCTTTCGGTGACTACTTTCCTGGCGTGCAAAATCCCCTTGATGGTGTACAATGGACGCAGGAAAGACCAAGTGGTGTATACCAGTATTTCATCAAGGTGGTCCCTACGATATACACCAATATTATAGGCCGTACAATTTTCTCAAATCAGTACTCTGTGACAGAGCATTATAAGAGTTCGGAGCTGGGACACTCTCAAATGCTTCCAGGAGTTTTCTTCTTTTATGATCTTTCTCCAATTAAGGTCACATTCAAAGAAGAACATGTTCCGTTTTTACACTTCATGACCCATATTTGTGCCATAATTGGAGGTATATTTACCATTGCAGGACTTATAGATTCGTTTATATACTATGGTCAAAGAGCAATTAAGAAAAAGATCGAAGCTGGAAAATTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

390

Amino Acids

43.85

Weight (kDa)

6.31

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 11 - 100 3.3e-30 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 152 - 369 2.3e-83 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22200 AT1G22200
malus_domestica MD05G1045400.v1.1 MD10G1052300.v1.1
prunus_persica Prupe.8G062600_v2.0.a1
pyrus_communis pycom10g03700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
Acc16I TGCGCA 1 cut(s) 342
AccI GTMKAC 1 cut(s) 846
AciI CCGC 2 cut(s) 12, 288
AclWI GGATC 3 cut(s) 457, 624, 637
AcsI RAATTY 2 cut(s) 658, 1160
AcuI CTGAAG 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 4 cut(s) 82, 816, 901, 920
AfiI CCNNNNNNNGG 2 cut(s) 408, 809
AflIII ACRYGT 1 cut(s) 1030
AgsI TTSAA 6 cut(s) 201, 428, 587, 626, 638, 1021
AjnI CCWGG 3 cut(s) 673, 787, 973
AluBI AGCT 7 cut(s) 31, 148, 184, 362, 770, 952, 1154
AluI AGCT 7 cut(s) 31, 148, 184, 362, 770, 952, 1154
Alw21I GWGCWC 2 cut(s) 150, 385
Alw44I GTGCAC 1 cut(s) 381
AlwI GGATC 3 cut(s) 457, 624, 637
AoxI GGCC 1 cut(s) 895
ApaLI GTGCAC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 2 cut(s) 658, 1160
Asp700I GAANNNNTTC 1 cut(s) 1032
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 868
AsuHPI GGTGA 3 cut(s) 620, 680, 788
AvaII GGWCC 1 cut(s) 868
BaeGI GKGCMC 2 cut(s) 385, 538
BamHI GGATCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 1 cut(s) 493
Bbv12I GWGCWC 2 cut(s) 150, 385
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 307
BccI CCATC 3 cut(s) 365, 599, 803
BceAI ACGGC 2 cut(s) 63, 882
BciT130I CCWGG 3 cut(s) 675, 789, 975
BciVI GTATCC 1 cut(s) 29
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
BmcAI AGTACT 1 cut(s) 920
Bme1390I CCNGG 3 cut(s) 675, 789, 975
Bme18I GGWCC 1 cut(s) 868
BmgT120I GGNCC 1 cut(s) 868
BmiI GGNNCC 2 cut(s) 631, 870
BmrFI CCNGG 3 cut(s) 675, 789, 975
BmsI GCATC 5 cut(s) 105, 184, 242, 328, 502
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 2 cut(s) 408, 809
Bse1I ACTGG 3 cut(s) 562, 691, 850
Bse3DI GCAATG 1 cut(s) 1089
BseBI CCWGG 3 cut(s) 675, 789, 975
BseGI GGATG 2 cut(s) 376, 620
BseLI CCNNNNNNNGG 2 cut(s) 408, 809
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 3 cut(s) 23, 163, 277
BseNI ACTGG 3 cut(s) 562, 691, 850
BseRI GAGGAG 3 cut(s) 49, 110, 219
BseSI GKGCMC 2 cut(s) 385, 538
BseXI GCAGC 1 cut(s) 307
BseYI CCCAGC 1 cut(s) 952
BshFI GGCC 1 cut(s) 897
BsiHKAI GWGCWC 2 cut(s) 150, 385
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 854, 969
BslI CCNNNNNNNGG 2 cut(s) 408, 809
BsmFI GGGAC 2 cut(s) 854, 969
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 1 cut(s) 897
Bsp1286I GDGCHC 3 cut(s) 150, 385, 538
Bsp1407I TGTACA 1 cut(s) 814
Bsp143I GATC 4 cut(s) 449, 629, 994, 1146
BspACI CCGC 2 cut(s) 12, 288
BspANI GGCC 1 cut(s) 897
BspCNI CTCAG 3 cut(s) 24, 162, 276
BspHI TCATGA 1 cut(s) 1050
BspLI GGNNCC 2 cut(s) 631, 870
BspPI GGATC 3 cut(s) 457, 624, 637
BspQI GCTCTTC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 3 cut(s) 562, 691, 850
BssMI GATC 4 cut(s) 449, 629, 994, 1146
BssNAI GTATAC 1 cut(s) 847
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 493
Bst1107I GTATAC 1 cut(s) 847
Bst2BI CACGAG 1 cut(s) 493
Bst2UI CCWGG 3 cut(s) 675, 789, 975
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 2 cut(s) 118, 134
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 2 cut(s) 495, 715
BstAUI TGTACA 1 cut(s) 814
BstC8I GCNNGC 2 cut(s) 720, 794
BstDEI CTNAG 4 cut(s) 32, 149, 263, 363
BstENI CCTNNNNNAGG 1 cut(s) 406
BstF5I GGATG 2 cut(s) 376, 620
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 4 cut(s) 452, 632, 997, 1149
BstMBI GATC 4 cut(s) 449, 629, 994, 1146
BstMWI GCNNNNNNNGC 2 cut(s) 495, 715
BstNI CCWGG 3 cut(s) 675, 789, 975
BstNSI RCATGY 1 cut(s) 1034
BstSCI CCNGG 3 cut(s) 673, 787, 973
BstSLI GKGCMC 2 cut(s) 385, 538
BstV1I GCAGC 1 cut(s) 307
BstX2I RGATCY 1 cut(s) 629
BstYI RGATCY 1 cut(s) 629
BstZ17I GTATAC 1 cut(s) 847
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 897
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 376, 620
BtsIMutI CAGTG 3 cut(s) 165, 536, 569
Cac8I GCNNGC 2 cut(s) 720, 794
CciI TCATGA 1 cut(s) 1050
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 868
CseI GACGC 2 cut(s) 53, 832
Csp6I GTAC 4 cut(s) 81, 815, 900, 919
CviAII CATG 6 cut(s) 124, 344, 413, 710, 1031, 1051
CviQI GTAC 4 cut(s) 81, 815, 900, 919
DdeI CTNAG 4 cut(s) 32, 149, 263, 363
DpnI GATC 4 cut(s) 451, 631, 996, 1148
DpnII GATC 4 cut(s) 449, 629, 994, 1146
Eam1104I CTCTTC 2 cut(s) 118, 134
EarI CTCTTC 2 cut(s) 118, 134
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 868
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 406
EcoRII CCWGG 3 cut(s) 673, 787, 973
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 6 cut(s) 127, 347, 416, 713, 1034, 1054
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 2 cut(s) 854, 969
FatI CATG 6 cut(s) 123, 343, 412, 709, 1030, 1050
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 2 cut(s) 383, 627
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 296
FspAI RTGCGCAY 1 cut(s) 342
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 1 cut(s) 296
GsaI CCCAGC 1 cut(s) 956
HaeIII GGCC 1 cut(s) 897
HapII CCGG 1 cut(s) 90
HgaI GACGC 2 cut(s) 53, 832
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 6 cut(s) 127, 347, 416, 713, 1034, 1054
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 2 cut(s) 176, 1106
HpaI GTTAAC 1 cut(s) 643
HpaII CCGG 1 cut(s) 90
HphI GGTGA 3 cut(s) 620, 680, 788
Hpy166II GTNNAC 5 cut(s) 83, 383, 643, 815, 847
Hpy188I TCNGA 5 cut(s) 145, 152, 251, 475, 949
Hpy188III TCNNGA 6 cut(s) 201, 269, 325, 509, 727, 1051
Hpy8I GTNNAC 5 cut(s) 83, 383, 643, 815, 847
HpyAV CCTTC 5 cut(s) 361, 414, 569, 605, 620
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 8 cut(s) 383, 406, 489, 515, 548, 709, 796, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 495, 715
HpyF3I CTNAG 4 cut(s) 32, 149, 263, 363
HpySE526I ACGT 1 cut(s) 470
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 6 cut(s) 127, 347, 416, 713, 1034, 1054
HspAI GCGC 1 cut(s) 341
KspAI GTTAAC 1 cut(s) 643
Kzo9I GATC 4 cut(s) 449, 629, 994, 1146
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 2 cut(s) 461, 949
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 5 cut(s) 105, 184, 242, 328, 502
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 7 cut(s) 169, 232, 737, 749, 776, 925, 1012
MalI GATC 4 cut(s) 451, 631, 996, 1148
MboI GATC 4 cut(s) 449, 629, 994, 1146
MboII GAAGA 7 cut(s) 64, 105, 121, 130, 614, 976, 1037
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 3 cut(s) 150, 385, 538
MluCI AATT 9 cut(s) 223, 333, 375, 658, 903, 1005, 1071, 1134, 1160
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 8 cut(s) 27, 116, 121, 128, 131, 170, 197, 1070
Mph1103I ATGCAT 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 1032
MseI TTAA 3 cut(s) 642, 1008, 1137
MslI CAYNNNNRTG 4 cut(s) 348, 708, 863, 1062
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 3 cut(s) 675, 789, 975
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 3 cut(s) 675, 789, 975
MwoI GCNNNNNNNGC 2 cut(s) 495, 715
NdeII GATC 4 cut(s) 449, 629, 994, 1146
NlaIII CATG 6 cut(s) 127, 347, 416, 713, 1034, 1054
NlaIV GGNNCC 2 cut(s) 631, 870
NmuCI GTSAC 4 cut(s) 749, 776, 925, 1012
NsbI TGCGCA 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 1 cut(s) 1034
PagI TCATGA 1 cut(s) 1050
PciI ACATGT 1 cut(s) 1030
PciSI GCTCTTC 1 cut(s) 134
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 1032
PfeI GAWTC 1 cut(s) 1106
PfoI TCCNGGA 1 cut(s) 973
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PscI ACATGT 1 cut(s) 1030
PsiI TTATAA 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 3 cut(s) 673, 787, 973
PspFI CCCAGC 1 cut(s) 952
PspGI CCWGG 3 cut(s) 673, 787, 973
PspN4I GGNNCC 2 cut(s) 631, 870
PspPI GGNCC 1 cut(s) 868
PsuI RGATCY 1 cut(s) 629
RsaI GTAC 4 cut(s) 82, 816, 901, 920
RsaNI GTAC 4 cut(s) 81, 815, 900, 919
RseI CAYNNNNRTG 4 cut(s) 348, 708, 863, 1062
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 642, 1008, 1137
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 4 cut(s) 449, 629, 994, 1146
Sau96I GGNCC 1 cut(s) 868
ScaI AGTACT 1 cut(s) 920
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 675, 789, 975
SduI GDGCHC 3 cut(s) 150, 385, 538
SfaNI GCATC 5 cut(s) 105, 184, 242, 328, 502
SinI GGWCC 1 cut(s) 868
SmiMI CAYNNNNRTG 4 cut(s) 348, 708, 863, 1062
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 9 cut(s) 223, 333, 375, 658, 903, 1005, 1071, 1134, 1160
SsiI CCGC 2 cut(s) 12, 288
SspI AATATT 3 cut(s) 443, 622, 889
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 3 cut(s) 673, 787, 973
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 473
TaqI TCGA 1 cut(s) 1149
TasI AATT 9 cut(s) 223, 333, 375, 658, 903, 1005, 1071, 1134, 1160
TatI WGTACW 2 cut(s) 814, 918
TfiI GAWTC 1 cut(s) 1106
Tru1I TTAA 3 cut(s) 642, 1008, 1137
Tru9I TTAA 3 cut(s) 642, 1008, 1137
TscAI CASTG 3 cut(s) 172, 543, 569
TseFI GTSAC 4 cut(s) 749, 776, 925, 1012
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 4 cut(s) 749, 776, 925, 1012
TspDTI ATGAA 7 cut(s) 450, 516, 615, 653, 692, 847, 1039
TspGWI ACGGA 1 cut(s) 1026
TspRI CASTG 3 cut(s) 172, 543, 569
VneI GTGCAC 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 868
XagI CCTNNNNNAGG 1 cut(s) 406
XapI RAATTY 2 cut(s) 658, 1160
XceI RCATGY 1 cut(s) 1034
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 1 cut(s) 1032
ZrmI AGTACT 1 cut(s) 920
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.