MD10G1052300.v1.1

Endoplasmic reticulum-Golgi intermediate compartment protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0020163
Physical Location & Seq
Reverse (-)
6925646 .. 6934925
9280 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1052300.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGGCAGGGACGGTGGTATCCTTGAGAAGCTGAGGGGTTTGGACGCCTACCCTAAAATCAATGACGATTTCTTCAGCCGTACGCTCTCCGGCGGCATCATTACCCTCGCATCTTCCCTCCTTATGTTCTTCCTCTTCGTCTCCGAGCTCGGACTATACCTCCACACTGTTACAGAGTCAAAGCTTCTTGTAGATGTTTCAAGAGGAGAAACCCTACATATCAATTTTGATGTGACTTTTCCAGCTATCCGATGCTCATTACTCAGTCTTGACACAATGGATATTAGTGGAGAGCGGCATTTTGACATACGACACGATATAGTCAAGAAACGAATTGATGCGCATGGTAATGTAATAGAAGCTAAGAAGGATGGAATTGGTGCACCAAAGATTGAGAGCCCCTTGCAAAGGCATGGTGGAAGGCTTGAGCATAATGAAAAATATTGTGGATCGTGCTTTGGAGCAGAAACGTCTGATGATGAATGTTGCAACTCGTGTGATGAAGTTCAAGAGGCATATAAGAAAAAAGGTTGGGCATTGACGAATGCAGATTTGATAGACCAGTGCAAACGGGAAGGTTTTATTCAAAAGATAAAAGATGAAGATGGTGAAGGATGTAATATTGAAGGATCCCTTGCAGTTAACAAAGTCGCGGGAAATTTTCATTTTATGCCTGGAAAAAGTTTTCACCAGTCTAACTTTCATGTCCATGATTTGCTGGCTTTCCAGACAGATAGTTACAATATGAGTCACAAGATAAACAGATTAGCTTTTGGTGACCACTTTCCTGGCATGCAAAATCCCCTTGATCGTGTACAATGGATGCAGGAAAGACCTGGTGGTATGTACCAGTATTTCATCAAGGTGGTCCCTACGCTATACACCAATATTAGAGGCCGTACAATTCGCTCAAATCAGTATTCTGTGACAGAGCATTATAAGAGTTCAGAGCTGGGACACTCTCAATTGCCACCAGGAGTTTTCTTCTATTATGATCTTTCTCCAATTAAGGTCACATTCAAAGAAGAACATGTTCCGTTTTTACACTTCGTGACCCATATCTGTGCCATAGTTGGAGGTATATTTACCATTGCAGGACTTGTGGATGCGTTTATATATCACGGTCAAAGAGCAATTAGGAAGATTGAAGCTGGAAAATTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

389

Amino Acids

43.74

Weight (kDa)

6.44

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 11 - 100 1.8e-29 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 152 - 369 2.1e-84 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22200 AT1G22200
malus_domestica MD05G1045400.v1.1 MD10G1052300.v1.1
prunus_persica Prupe.8G062600_v2.0.a1
pyrus_communis pycom10g03700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
Acc16I TGCGCA 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 295
AccII CGCG 1 cut(s) 653
AciI CCGC 3 cut(s) 93, 295, 653
AclWI GGATC 3 cut(s) 457, 624, 637
AcsI RAATTY 2 cut(s) 658, 1157
AcuI CTGAAG 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 45
AdeI CACNNNGTG 1 cut(s) 1051
AfaI GTAC 4 cut(s) 82, 816, 848, 901
AfiI CCNNNNNNNGG 1 cut(s) 408
AflIII ACRYGT 1 cut(s) 1030
AgsI TTSAA 6 cut(s) 201, 509, 587, 626, 1021, 1148
AjnI CCWGG 4 cut(s) 673, 787, 835, 973
AluBI AGCT 8 cut(s) 31, 148, 184, 245, 362, 770, 952, 1151
AluI AGCT 8 cut(s) 31, 148, 184, 245, 362, 770, 952, 1151
Alw21I GWGCWC 2 cut(s) 150, 385
Alw26I GTCTC 1 cut(s) 145
Alw44I GTGCAC 1 cut(s) 381
AlwI GGATC 3 cut(s) 457, 624, 637
AoxI GGCC 1 cut(s) 895
ApaLI GTGCAC 1 cut(s) 381
ApoI RAATTY 2 cut(s) 658, 1157
Asp700I GAANNNNTTC 1 cut(s) 1032
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 868
AsuHPI GGTGA 3 cut(s) 620, 680, 788
AvaII GGWCC 1 cut(s) 868
BaeGI GKGCMC 1 cut(s) 385
BaeI ACNNNNGTAYC 1 cut(s) 34
BamHI GGATCC 1 cut(s) 629
BanII GRGCYC 2 cut(s) 150, 401
BauI CACGAG 1 cut(s) 493
Bbv12I GWGCWC 2 cut(s) 150, 385
BbvCI CCTCAGC 1 cut(s) 32
BccI CCATC 2 cut(s) 365, 599
BceAI ACGGC 2 cut(s) 63, 882
BciT130I CCWGG 4 cut(s) 675, 789, 837, 975
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 145
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 2 cut(s) 94, 296
BlsI GCNGC 2 cut(s) 95, 297
Bme1390I CCNGG 4 cut(s) 675, 789, 837, 975
Bme18I GGWCC 1 cut(s) 868
BmgT120I GGNCC 1 cut(s) 868
BmiI GGNNCC 2 cut(s) 631, 870
BmrFI CCNGG 4 cut(s) 675, 789, 837, 975
BmsI GCATC 6 cut(s) 105, 119, 242, 328, 813, 1096
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 44, 446
BsaHI GRCGYC 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse1I ACTGG 3 cut(s) 562, 691, 850
Bse3DI GCAATG 1 cut(s) 1089
BseBI CCWGG 4 cut(s) 675, 789, 837, 975
BseGI GGATG 4 cut(s) 376, 620, 828, 1111
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 2 cut(s) 23, 277
BseNI ACTGG 3 cut(s) 562, 691, 850
BseRI GAGGAG 1 cut(s) 219
BseSI GKGCMC 1 cut(s) 385
BseYI CCCAGC 1 cut(s) 952
Bsh1236I CGCG 1 cut(s) 653
BshFI GGCC 1 cut(s) 897
BsiHKAI GWGCWC 2 cut(s) 150, 385
BsiSI CCGG 1 cut(s) 90
BsiWI CGTACG 1 cut(s) 80
BslFI GGGAC 3 cut(s) 23, 854, 969
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 145
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 3 cut(s) 23, 854, 969
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 1 cut(s) 897
Bsp1286I GDGCHC 3 cut(s) 150, 385, 401
Bsp1407I TGTACA 1 cut(s) 814
Bsp143I GATC 4 cut(s) 449, 629, 808, 994
BspACI CCGC 3 cut(s) 93, 295, 653
BspANI GGCC 1 cut(s) 897
BspCNI CTCAG 2 cut(s) 24, 276
BspFNI CGCG 1 cut(s) 653
BspLI GGNNCC 2 cut(s) 631, 870
BspPI GGATC 3 cut(s) 457, 624, 637
BsrBI CCGCTC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 3 cut(s) 562, 691, 850
BssMI GATC 4 cut(s) 449, 629, 808, 994
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 493
Bst2UI CCWGG 4 cut(s) 675, 789, 837, 975
Bst4CI ACNGT 3 cut(s) 14, 169, 1124
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 45
BstAUI TGTACA 1 cut(s) 814
BstC8I GCNNGC 2 cut(s) 720, 794
BstDEI CTNAG 3 cut(s) 32, 263, 363
BstEII GGTNACC 1 cut(s) 776
BstENI CCTNNNNNAGG 1 cut(s) 406
BstF5I GGATG 4 cut(s) 376, 620, 828, 1111
BstFNI CGCG 1 cut(s) 653
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 4 cut(s) 452, 632, 811, 997
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 4 cut(s) 449, 629, 808, 994
BstNI CCWGG 4 cut(s) 675, 789, 837, 975
BstNSI RCATGY 2 cut(s) 796, 1034
BstPI GGTNACC 1 cut(s) 776
BstSCI CCNGG 4 cut(s) 673, 787, 835, 973
BstSLI GKGCMC 1 cut(s) 385
BstUI CGCG 1 cut(s) 653
BstX2I RGATCY 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 788
BstYI RGATCY 1 cut(s) 629
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 897
BtsCI GGATG 4 cut(s) 376, 620, 828, 1111
BtsIMutI CAGTG 2 cut(s) 165, 569
Cac8I GCNNGC 2 cut(s) 720, 794
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 868
CseI GACGC 1 cut(s) 53
CsiI ACCWGGT 1 cut(s) 835
Csp6I GTAC 4 cut(s) 81, 815, 847, 900
CviAII CATG 6 cut(s) 344, 413, 704, 710, 793, 1031
CviQI GTAC 4 cut(s) 81, 815, 847, 900
DdeI CTNAG 3 cut(s) 32, 263, 363
DpnI GATC 4 cut(s) 451, 631, 810, 996
DpnII GATC 4 cut(s) 449, 629, 808, 994
DraIII CACNNNGTG 1 cut(s) 1051
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 2 cut(s) 150, 401
Eco47I GGWCC 1 cut(s) 868
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 58
Eco91I GGTNACC 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 406
EcoO65I GGTNACC 1 cut(s) 776
EcoRII CCWGG 4 cut(s) 673, 787, 835, 973
EcoT38I GRGCYC 2 cut(s) 150, 401
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 6 cut(s) 347, 416, 707, 713, 796, 1034
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 3 cut(s) 23, 854, 969
FatI CATG 6 cut(s) 343, 412, 703, 709, 792, 1030
FauI CCCGC 1 cut(s) 646
Fnu4HI GCNGC 2 cut(s) 94, 296
FokI GGATG 4 cut(s) 383, 627, 835, 1118
FriOI GRGCYC 2 cut(s) 150, 401
Fsp4HI GCNGC 2 cut(s) 94, 296
FspAI RTGCGCAY 1 cut(s) 342
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 2 cut(s) 94, 296
GsaI CCCAGC 1 cut(s) 956
HaeIII GGCC 1 cut(s) 897
HapII CCGG 1 cut(s) 90
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 6 cut(s) 347, 416, 707, 713, 796, 1034
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 2 cut(s) 176, 748
HpaI GTTAAC 1 cut(s) 643
HpaII CCGG 1 cut(s) 90
HphI GGTGA 3 cut(s) 620, 680, 788
Hpy166II GTNNAC 3 cut(s) 383, 643, 815
Hpy188I TCNGA 5 cut(s) 145, 152, 251, 475, 949
Hpy188III TCNNGA 6 cut(s) 201, 269, 325, 509, 727, 1051
Hpy8I GTNNAC 3 cut(s) 383, 643, 815
HpyAV CCTTC 5 cut(s) 361, 414, 569, 605, 620
HpyCH4III ACNGT 3 cut(s) 14, 169, 1124
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 9 cut(s) 383, 406, 489, 548, 567, 638, 796, 826, 1094
HpyF3I CTNAG 3 cut(s) 32, 263, 363
HpySE526I ACGT 1 cut(s) 470
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 6 cut(s) 347, 416, 707, 713, 796, 1034
HspAI GCGC 1 cut(s) 341
KspAI GTTAAC 1 cut(s) 643
Kzo9I GATC 4 cut(s) 449, 629, 808, 994
LmnI GCTCC 1 cut(s) 461
LweI GCATC 6 cut(s) 105, 119, 242, 328, 813, 1096
MabI ACCWGGT 1 cut(s) 835
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 8 cut(s) 169, 232, 737, 749, 776, 925, 1012, 1051
MalI GATC 4 cut(s) 451, 631, 810, 996
MbiI CCGCTC 1 cut(s) 295
MboI GATC 4 cut(s) 449, 629, 808, 994
MboII GAAGA 8 cut(s) 64, 105, 121, 127, 614, 976, 1037, 1153
MfeI CAATTG 1 cut(s) 965
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 3 cut(s) 150, 385, 401
MluCI AATT 9 cut(s) 223, 333, 375, 658, 903, 965, 1005, 1134, 1157
MlyI GAGTC 2 cut(s) 185, 757
MmeI TCCRAC 1 cut(s) 1054
MnlI CCTC 9 cut(s) 27, 116, 128, 143, 170, 197, 505, 887, 1070
MroXI GAANNNNTTC 1 cut(s) 1032
MseI TTAA 2 cut(s) 642, 1008
MslI CAYNNNNRTG 4 cut(s) 348, 708, 863, 1062
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 4 cut(s) 675, 789, 837, 975
MunI CAATTG 1 cut(s) 965
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 4 cut(s) 675, 789, 837, 975
MvnI CGCG 1 cut(s) 653
NdeII GATC 4 cut(s) 449, 629, 808, 994
NlaIII CATG 6 cut(s) 347, 416, 707, 713, 796, 1034
NlaIV GGNNCC 2 cut(s) 631, 870
NmuCI GTSAC 6 cut(s) 232, 749, 776, 925, 1012, 1051
NsbI TGCGCA 1 cut(s) 342
NspI RCATGY 2 cut(s) 796, 1034
PaeI GCATGC 1 cut(s) 796
PciI ACATGT 1 cut(s) 1030
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 1032
Pfl23II CGTACG 1 cut(s) 80
PkrI GCNGC 2 cut(s) 95, 297
PleI GAGTC 2 cut(s) 184, 756
PpsI GAGTC 2 cut(s) 184, 756
PscI ACATGT 1 cut(s) 1030
PsiI TTATAA 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 4 cut(s) 673, 787, 835, 973
PspEI GGTNACC 1 cut(s) 776
PspFI CCCAGC 1 cut(s) 952
PspGI CCWGG 4 cut(s) 673, 787, 835, 973
PspLI CGTACG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 631, 870
PspPI GGNCC 1 cut(s) 868
PsuI RGATCY 1 cut(s) 629
RsaI GTAC 4 cut(s) 82, 816, 848, 901
RsaNI GTAC 4 cut(s) 81, 815, 847, 900
RseI CAYNNNNRTG 4 cut(s) 348, 708, 863, 1062
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 2 cut(s) 642, 1008
SatI GCNGC 2 cut(s) 94, 296
Sau3AI GATC 4 cut(s) 449, 629, 808, 994
Sau96I GGNCC 1 cut(s) 868
SchI GAGTC 2 cut(s) 185, 757
ScrFI CCNGG 4 cut(s) 675, 789, 837, 975
SduI GDGCHC 3 cut(s) 150, 385, 401
SexAI ACCWGGT 1 cut(s) 835
SfaNI GCATC 6 cut(s) 105, 119, 242, 328, 813, 1096
SinI GGWCC 1 cut(s) 868
SmiMI CAYNNNNRTG 4 cut(s) 348, 708, 863, 1062
SmlI CTYRAG 2 cut(s) 23, 425
SmoI CTYRAG 2 cut(s) 23, 425
SphI GCATGC 1 cut(s) 796
Sse9I AATT 9 cut(s) 223, 333, 375, 658, 903, 965, 1005, 1134, 1157
SsiI CCGC 3 cut(s) 93, 295, 653
SspI AATATT 3 cut(s) 443, 622, 889
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 4 cut(s) 673, 787, 835, 973
TaaI ACNGT 3 cut(s) 14, 169, 1124
TaiI ACGT 1 cut(s) 473
TasI AATT 9 cut(s) 223, 333, 375, 658, 903, 965, 1005, 1134, 1157
TatI WGTACW 1 cut(s) 814
TauI GCSGC 2 cut(s) 96, 298
Tru1I TTAA 2 cut(s) 642, 1008
Tru9I TTAA 2 cut(s) 642, 1008
TscAI CASTG 2 cut(s) 172, 569
TseFI GTSAC 6 cut(s) 232, 749, 776, 925, 1012, 1051
Tsp45I GTSAC 6 cut(s) 232, 749, 776, 925, 1012, 1051
TspDTI ATGAA 7 cut(s) 450, 495, 516, 615, 653, 692, 847
TspGWI ACGGA 1 cut(s) 1026
TspRI CASTG 2 cut(s) 172, 569
VneI GTGCAC 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 868
XagI CCTNNNNNAGG 1 cut(s) 406
XapI RAATTY 2 cut(s) 658, 1157
XceI RCATGY 2 cut(s) 796, 1034
XmnI GAANNNNTTC 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.