Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009128

Physical Location & Seq

Genomic Coordinates Forward (+)

10485633 .. 10492407

Gene Structure 6775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1060400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1935 bp

ATGGCTTCCCGGAAACACTTGGCCGCTGTTAAGGGCGCGAAAGTGCTCATGGTTGGGGCAGGTGGTATCGGCTGCGAGCTTCTCAAGACTCTCGCTCTCTCTGGCTTCCAAGATATTCATATTATCGACATGGACACTATAGAAGTGAGTAACCTCAACAGACAATTCCTCTTCCGACAATCACATGTTGGCCAATCCAAAGCCAAAGTTGCTCGGGAAGCTGTCTTAAAATTTAGGCCTGAGATAAGCATAACTTCATACCATGCAAATGTTAAGGATTCTGACTTCAATGTGGACTTCTTTAAGCAATTTAATGTGGTTTTGAATGGACTTGACAACTTAGATGCAAGGCGACATGTGAATCGCCTATGCTTGGGAGCTGATGTTCCCTTGATTGAAAGTGGGACGACTGGGTTCCTTGGACAGGTGACTGTACATGTGAAGGGAAGAACAGAGTGCTATGAGTGTCAACCAAAACCTGCTCCTAAAACTTATCCTGTCTGTACCATTACCAGTACTCCATCAAAGTTTGTTCACTGTATCGTATGGGCAAAGGAACTTCTTTTTGCAAAGTTGTTTGGGGACAAGAATCAGGCAAATGATTTGAATGTGCGTTCTAGTGATGGTGCTAGCTCATCAAAACAGGCTGAAGATGTATTTGAACATAGAGATGATGAAGACATTGAGCAGTATGGAAGGAGAATATATGATCATGTATTTGGTTATAATATTGAGCAAGCTTTGTCTAATGAGGAGACATGGAAGAACCGCAATAGACCAAAGCCTATGTATATTAGGGATGTCATGCTCAATGAGCTCGGTCAACAGAATGGAAGTGTGGAAAAGAATGGTGCAGCCAATGAAGTATATTCAGTATCTGCCATGGCATCTCTAGGAATGAAGAATCCACAGGATATATGGAGCCTTATGGAAAATTCCAGAGTCTTTCTTGAGGCTTTGAAACTATTTTTCATGAAAAGGCAGAAGGAAATTGGAAATCTCACCTTTGATAAAGATGATCAGCTAGCTGTGGAGTTTGTTACTGCTGCTGCAAATATACGGGCTGCTTCTTTTGGAATCCCGTTGCATAGCCTTTTTGAAGCTAAAGGGATTGCTGGTAATATTGTACATGCTGTTGCGACAACGAATGCTATTATTGCTGGGTTGATTGTCATTGAGGCAATCAAGGTGCTGCAAAATGATACAAAGAACTATAGGATGACATATTGTCTAGAACATCCAGCAAGAAAAATGTTGCTCATGCCAGTGGATCCATTTGAGCCTAACAAGTCTTGCTATGTCTGTTCAGAGACACCACTATCACTTGAGATCAATACTCACCGTACAAAGTTGCGGGATGTAGTTGAAAAGATTGTTAGAGCCACGCTCGGGATGGGCTTGCCACTCATTATGCATGGTTCAGCCCTTCTTTATGAAGTTGGTGATGACTTGGACGAAGACATGGTAGCAAATTATGCAGCAAACCTTGATAAGGTGCTGTCTGAGCTTCCTACACCAGTTACCAGTGGGACCATGCTGACAATTGAGGATTTTCTGCAAGAACTGTCGTGCAAGATCAATATTAAACACAGAGAGGAATTTGATGAAGAGAAAGAACCCGATGGAATGGTTCTATCTGGGTGGACTCAAGCTCCTGCAGTGGCACAGGATGACAAGAAGTCTACTGCAAATGCTGGAAGTTCATCAAGTGCATCATCATCTATGGAGCCTGAAAAGACTGATGACATTGAAACTACTCCTGGAAAGAAAAGAAAACTGTCTGAGGTTTCTGAGTCCGCGAATCCAGAATTGTCGAGTGTTGCTGGCAGTAGTACAAGAAATCCCGAGAAATTGGAACTGGTTGATGACGACGATGACCTTGTCATATTTGATAATTGGGATTCGGTCACGGACAAGAAGATAAAGTTGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008641 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016874 GO:0016877 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0019948 GO:0022414 GO:0030054 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0061458 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

ThiF UAE_UbL UBA_e1_thiolCys

Protein Analysis

645

Amino Acids

71.45

Weight (kDa)

5.38

Isoelectric Point (pI)

41.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	8 - 432	3.9e-65	ThiF family
UAE_UbL	PF14732	442 - 534	6.4e-23	Ubiquitin/SUMO-activating enzyme ubiquitin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21470 AT2G21470
fragaria_vesca	FvH4_2g09750 FvH4_2g09750 FvH4_2g09750
malus_domestica	MD05G1060400.v1.1 MD10G1068200.v1.1
prunus_persica	Prupe.8G090100_v2.0.a1
pyrus_communis	pycom05g05140 pycom10g05400
rosa_chinensis	RchiOBHm_Chr6g0266041
rosa_laevigata	RLG00000014122
rosa_multiflora	Rmu_sc0000776.1_g000024
rosa_roxburghii	Rroxscaffold_174G00435160 Rroxscaffold_7G00202040
rosa_rugosa	Rorug06G0022900 Rorug06G0022900
rosa_samantha	Rh6AG144100 Rh6BG144600 Rh6CG141100 Rh6DG129000
rosa_wichuraiana	Rw6G012450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	726
AarI	CACCTGC	1 cut(s)	50
Acc36I	ACCTGC	2 cut(s)	50, 487
AccI	GTMKAC	1 cut(s)	1682
AccII	CGCG	2 cut(s)	38, 1799
AciI	CCGC	4 cut(s)	24, 769, 1354, 1797
AclWI	GGATC	2 cut(s)	1265, 1278
AcoI	YGGCCR	2 cut(s)	21, 190
AcsI	RAATTY	3 cut(s)	230, 934, 1598
AcuI	CTGAAG	1 cut(s)	669
AfaI	GTAC	6 cut(s)	435, 505, 517, 1128, 1345, 1834
AfiI	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
AflIII	ACRYGT	3 cut(s)	184, 355, 436
AgsI	TTSAA	9 cut(s)	289, 325, 398, 607, 662, 961, 1100, 1367, 1751
AhdI	GACNNNNNGTC	2 cut(s)	1227, 1678
AjnI	CCWGG	1 cut(s)	1759
AloI	GAACNNNNNNTCC	2 cut(s)	369, 401
Alw21I	GWGCWC	2 cut(s)	48, 819
Alw26I	GTCTC	2 cut(s)	749, 1304
AlwI	GGATC	2 cut(s)	1265, 1278
AlwNI	CAGNNNCTG	1 cut(s)	878
Ama87I	CYCGRG	3 cut(s)	213, 1388, 1844
AoxI	GGCC	3 cut(s)	21, 190, 236
ApeKI	GCWGC	7 cut(s)	72, 854, 1046, 1049, 1064, 1192, 1478
ApoI	RAATTY	3 cut(s)	230, 934, 1598
AspLEI	GCGC	1 cut(s)	38
AspS9I	GGNCC	1 cut(s)	1530
AsuC2I	CCSGG	1 cut(s)	10
AsuHPI	GGTGA	4 cut(s)	439, 994, 1331, 1454
AsuNHI	GCTAGC	2 cut(s)	629, 1024
AvaI	CYCGRG	3 cut(s)	213, 1388, 1844
AvaII	GGWCC	1 cut(s)	1530
BalI	TGGCCA	1 cut(s)	192
BamHI	GGATCC	1 cut(s)	1270
BanII	GRGCYC	1 cut(s)	819
BbsI	GAAGAC	2 cut(s)	684, 1464
Bbv12I	GWGCWC	2 cut(s)	48, 819
BbvI	GCAGC	7 cut(s)	59, 866, 1033, 1036, 1051, 1179, 1490
BccI	CCATC	4 cut(s)	529, 617, 1387, 1616
BciT130I	CCWGG	1 cut(s)	1761
BclI	TGATCA	2 cut(s)	709, 1018
BcnI	CCSGG	1 cut(s)	10
BcoDI	GTCTC	2 cut(s)	749, 1304
BfaI	CTAG	5 cut(s)	618, 630, 893, 1025, 1232
BfmI	CTRYAG	3 cut(s)	138, 1213, 1656
BfuAI	ACCTGC	2 cut(s)	50, 487
BisI	GCNGC	8 cut(s)	24, 73, 855, 1047, 1050, 1065, 1193, 1479
BlsI	GCNGC	8 cut(s)	25, 74, 856, 1048, 1051, 1066, 1194, 1480
BmcAI	AGTACT	1 cut(s)	517
Bme1390I	CCNGG	2 cut(s)	10, 1761
Bme18I	GGWCC	1 cut(s)	1530
BmeRI	GACNNNNNGTC	2 cut(s)	1227, 1678
BmeT110I	CYCGRG	3 cut(s)	213, 1388, 1844
BmgT120I	GGNCC	1 cut(s)	1530
BmiI	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1728
BmrFI	CCNGG	2 cut(s)	10, 1761
BmrI	ACTGGG	1 cut(s)	420
BmsI	GCATC	3 cut(s)	334, 896, 1721
BmtI	GCTAGC	2 cut(s)	633, 1028
BmuI	ACTGGG	1 cut(s)	420
BpiI	GAAGAC	2 cut(s)	684, 1464
BplI	GAGNNNNNCTC	2 cut(s)	1371, 1403
BpuEI	CTTGAG	4 cut(s)	68, 971, 1346, 1632
BpuMI	CCSGG	1 cut(s)	10
BsaJI	CCNNGG	2 cut(s)	418, 882
BsaXI	ACNNNNNCTCC	6 cut(s)	369, 399, 502, 532, 1636, 1666
Bsc4I	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
Bse1I	ACTGG	6 cut(s)	415, 513, 1265, 1517, 1524, 1863
BseBI	CCWGG	1 cut(s)	1761
BseDI	CCNNGG	2 cut(s)	418, 882
BseGI	GGATG	6 cut(s)	805, 1224, 1237, 1363, 1398, 1675
BseLI	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
BseMII	CTCAG	4 cut(s)	231, 1494, 1773, 1782
BseNI	ACTGG	6 cut(s)	415, 513, 1265, 1517, 1524, 1863
BseRI	GAGGAG	1 cut(s)	767
BseXI	GCAGC	7 cut(s)	59, 866, 1033, 1036, 1051, 1179, 1490
BseYI	CCCAGC	1 cut(s)	1160
BsgI	GTGCAG	1 cut(s)	873
Bsh1236I	CGCG	2 cut(s)	38, 1799
BshFI	GGCC	3 cut(s)	23, 192, 238
BsiHKAI	GWGCWC	2 cut(s)	48, 819
BsiHKCI	CYCGRG	3 cut(s)	213, 1388, 1844
BsiSI	CCGG	1 cut(s)	10
BslFI	GGGAC	3 cut(s)	418, 596, 1543
BslI	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
BsmAI	GTCTC	2 cut(s)	749, 1304
BsmFI	GGGAC	3 cut(s)	418, 596, 1543
BsmI	GAATGC	1 cut(s)	1153
BsnI	GGCC	3 cut(s)	23, 192, 238
BsoBI	CYCGRG	3 cut(s)	213, 1388, 1844
Bsp1286I	GDGCHC	2 cut(s)	48, 819
Bsp1407I	TGTACA	2 cut(s)	433, 1126
Bsp143I	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
Bsp19I	CCATGG	1 cut(s)	882
BspACI	CCGC	4 cut(s)	24, 769, 1354, 1797
BspANI	GGCC	3 cut(s)	23, 192, 238
BspCNI	CTCAG	4 cut(s)	232, 1495, 1774, 1783
BspFNI	CGCG	2 cut(s)	38, 1799
BspHI	TCATGA	1 cut(s)	972
BspLI	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1728
BspMAI	CTGCAG	1 cut(s)	1660
BspMI	ACCTGC	2 cut(s)	50, 487
BspOI	GCTAGC	2 cut(s)	633, 1028
BspPI	GGATC	2 cut(s)	1265, 1278
BsrGI	TGTACA	2 cut(s)	433, 1126
BsrI	ACTGG	6 cut(s)	415, 513, 1265, 1517, 1524, 1863
BssECI	CCNNGG	2 cut(s)	418, 882
BssMI	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
BssT1I	CCWWGG	2 cut(s)	418, 882
Bst2UI	CCWGG	1 cut(s)	1761
Bst4CI	ACNGT	5 cut(s)	433, 539, 1343, 1566, 1779
Bst6I	CTCTTC	2 cut(s)	176, 1602
BstAPI	GCANNNNNTGC	1 cut(s)	1475
BstAUI	TGTACA	2 cut(s)	433, 1126
BstC8I	GCNNGC	6 cut(s)	77, 631, 738, 1026, 1400, 1825
BstDEI	CTNAG	5 cut(s)	240, 340, 1503, 1782, 1791
BstDSI	CCRYGG	1 cut(s)	882
BstENI	CCTNNNNNAGG	2 cut(s)	422, 1490
BstF5I	GGATG	6 cut(s)	805, 1224, 1237, 1363, 1398, 1675
BstFNI	CGCG	2 cut(s)	38, 1799
BstHHI	GCGC	1 cut(s)	38
BstKTI	GATC	5 cut(s)	712, 1021, 1273, 1332, 1578
BstMAI	GTCTC	2 cut(s)	749, 1304
BstMBI	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
BstMWI	GCNNNNNNNGC	6 cut(s)	209, 218, 814, 1157, 1475, 1504
BstNI	CCWGG	1 cut(s)	1761
BstNSI	RCATGY	4 cut(s)	188, 359, 440, 1133
BstSCI	CCNGG	2 cut(s)	8, 1759
BstSFI	CTRYAG	3 cut(s)	138, 1213, 1656
BstUI	CGCG	2 cut(s)	38, 1799
BstV1I	GCAGC	7 cut(s)	59, 866, 1033, 1036, 1051, 1179, 1490
BstV2I	GAAGAC	2 cut(s)	684, 1464
BstX2I	RGATCY	1 cut(s)	1270
BstYI	RGATCY	1 cut(s)	1270
BsuRI	GGCC	3 cut(s)	23, 192, 238
BtgI	CCRYGG	1 cut(s)	882
BtsCI	GGATG	6 cut(s)	805, 1224, 1237, 1363, 1398, 1675
BtsI	GCAGTG	1 cut(s)	1665
BtsIMutI	CAGTG	4 cut(s)	535, 1272, 1531, 1665
BveI	ACCTGC	2 cut(s)	50, 487
Cac8I	GCNNGC	6 cut(s)	77, 631, 738, 1026, 1400, 1825
CaiI	CAGNNNCTG	1 cut(s)	878
CciI	TCATGA	1 cut(s)	972
CfoI	GCGC	1 cut(s)	38
Cfr13I	GGNCC	1 cut(s)	1530
Csp6I	GTAC	6 cut(s)	434, 504, 516, 1127, 1344, 1833
CviQI	GTAC	6 cut(s)	434, 504, 516, 1127, 1344, 1833
DdeI	CTNAG	5 cut(s)	240, 340, 1503, 1782, 1791
DpnI	GATC	5 cut(s)	711, 1020, 1272, 1331, 1577
DpnII	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
DriI	GACNNNNNGTC	2 cut(s)	1227, 1678
EaeI	YGGCCR	2 cut(s)	21, 190
Eam1104I	CTCTTC	2 cut(s)	176, 1602
Eam1105I	GACNNNNNGTC	2 cut(s)	1227, 1678
EarI	CTCTTC	2 cut(s)	176, 1602
Ecl136II	GAGCTC	1 cut(s)	817
Eco130I	CCWWGG	2 cut(s)	418, 882
Eco147I	AGGCCT	1 cut(s)	238
Eco24I	GRGCYC	1 cut(s)	819
Eco47I	GGWCC	1 cut(s)	1530
Eco53kI	GAGCTC	1 cut(s)	817
Eco57I	CTGAAG	1 cut(s)	669
Eco88I	CYCGRG	3 cut(s)	213, 1388, 1844
EcoICRI	GAGCTC	1 cut(s)	817
EcoNI	CCTNNNNNAGG	2 cut(s)	422, 1490
EcoRII	CCWGG	1 cut(s)	1759
EcoT14I	CCWWGG	2 cut(s)	418, 882
EcoT22I	ATGCAT	1 cut(s)	1416
EcoT38I	GRGCYC	1 cut(s)	819
ErhI	CCWWGG	2 cut(s)	418, 882
FalI	AAGNNNNNCTT	2 cut(s)	238, 270
FaqI	GGGAC	3 cut(s)	418, 596, 1543
FauI	CCCGC	1 cut(s)	1347
FbaI	TGATCA	2 cut(s)	709, 1018
FblI	GTMKAC	1 cut(s)	1682
Fnu4HI	GCNGC	8 cut(s)	24, 73, 855, 1047, 1050, 1065, 1193, 1479
FokI	GGATG	6 cut(s)	812, 1224, 1231, 1370, 1405, 1682
FriOI	GRGCYC	1 cut(s)	819
Fsp4HI	GCNGC	8 cut(s)	24, 73, 855, 1047, 1050, 1065, 1193, 1479
FspBI	CTAG	5 cut(s)	618, 630, 893, 1025, 1232
GlaI	GCGC	1 cut(s)	37
GluI	GCNGC	8 cut(s)	24, 73, 855, 1047, 1050, 1065, 1193, 1479
GsaI	CCCAGC	1 cut(s)	1164
HaeIII	GGCC	3 cut(s)	23, 192, 238
HapII	CCGG	1 cut(s)	10
HhaI	GCGC	1 cut(s)	38
Hin6I	GCGC	1 cut(s)	36
HinP1I	GCGC	1 cut(s)	36
HincII	GTYRAC	2 cut(s)	470, 824
HindII	GTYRAC	2 cut(s)	470, 824
HindIII	AAGCTT	1 cut(s)	738
HpaII	CCGG	1 cut(s)	10
HphI	GGTGA	4 cut(s)	439, 994, 1331, 1454
Hpy166II	GTNNAC	6 cut(s)	295, 470, 535, 824, 1644, 1683
Hpy188I	TCNGA	6 cut(s)	176, 283, 1309, 1504, 1783, 1792
Hpy188III	TCNNGA	9 cut(s)	85, 215, 939, 950, 973, 1232, 1390, 1805, 1844
Hpy8I	GTNNAC	6 cut(s)	295, 470, 535, 824, 1644, 1683
Hpy99I	CGWCG	1 cut(s)	1874
HpyAV	CCTTC	4 cut(s)	436, 690, 979, 1436
HpyCH4III	ACNGT	5 cut(s)	433, 539, 1343, 1566, 1779
HpyF10VI	GCNNNNNNNGC	6 cut(s)	209, 218, 814, 1157, 1475, 1504
HpyF3I	CTNAG	5 cut(s)	240, 340, 1503, 1782, 1791
HspAI	GCGC	1 cut(s)	36
Ksp22I	TGATCA	2 cut(s)	709, 1018
Kzo9I	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
LmnI	GCTCC	5 cut(s)	377, 487, 921, 1657, 1726
Lsp1109I	GCAGC	7 cut(s)	59, 866, 1033, 1036, 1051, 1179, 1490
LweI	GCATC	3 cut(s)	334, 896, 1721
MaeI	CTAG	5 cut(s)	618, 630, 893, 1025, 1232
MaeIII	GTNAC	5 cut(s)	149, 427, 1039, 1519, 1906
MalI	GATC	5 cut(s)	711, 1020, 1272, 1331, 1577
MboI	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
MboII	GAAGA	9 cut(s)	163, 459, 662, 689, 775, 913, 1469, 1619, 1930
MfeI	CAATTG	1 cut(s)	1542
MflI	RGATCY	1 cut(s)	1270
MhlI	GDGCHC	2 cut(s)	48, 819
MlsI	TGGCCA	1 cut(s)	192
MluNI	TGGCCA	1 cut(s)	192
MlyI	GAGTC	4 cut(s)	82, 951, 1639, 1802
MmeI	TCCRAC	1 cut(s)	199
MnlI	CCTC	8 cut(s)	164, 179, 745, 946, 1171, 1540, 1588, 1777
Mox20I	TGGCCA	1 cut(s)	192
Mph1103I	ATGCAT	1 cut(s)	1416
MscI	TGGCCA	1 cut(s)	192
MseI	TTAA	6 cut(s)	30, 227, 273, 303, 312, 1584
MslI	CAYNNNNRTG	3 cut(s)	267, 669, 1265
Msp20I	TGGCCA	1 cut(s)	192
MspA1I	CMGCKG	1 cut(s)	26
MspI	CCGG	1 cut(s)	10
MspR9I	CCNGG	2 cut(s)	10, 1761
MunI	CAATTG	1 cut(s)	1542
Mva1269I	GAATGC	1 cut(s)	1153
MvaI	CCWGG	1 cut(s)	1761
MvnI	CGCG	2 cut(s)	38, 1799
MwoI	GCNNNNNNNGC	6 cut(s)	209, 218, 814, 1157, 1475, 1504
NciI	CCSGG	1 cut(s)	10
NcoI	CCATGG	1 cut(s)	882
NdeII	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
NheI	GCTAGC	2 cut(s)	629, 1024
NlaIV	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1728
NmuCI	GTSAC	2 cut(s)	427, 1906
NsiI	ATGCAT	1 cut(s)	1416
NspI	RCATGY	4 cut(s)	188, 359, 440, 1133
PagI	TCATGA	1 cut(s)	972
PaqCI	CACCTGC	1 cut(s)	50
PceI	AGGCCT	1 cut(s)	238
PciI	ACATGT	3 cut(s)	184, 355, 436
PctI	GAATGC	1 cut(s)	1153
PfeI	GAWTC	7 cut(s)	278, 361, 589, 904, 1077, 1801, 1901
PflFI	GACNNNGTC	1 cut(s)	1880
PfoI	TCCNGGA	2 cut(s)	8, 1759
PkrI	GCNGC	8 cut(s)	25, 74, 856, 1048, 1051, 1066, 1194, 1480
PleI	GAGTC	4 cut(s)	82, 950, 1639, 1801
PpsI	GAGTC	4 cut(s)	82, 950, 1639, 1801
PscI	ACATGT	3 cut(s)	184, 355, 436
PsiI	TTATAA	1 cut(s)	726
Psp124BI	GAGCTC	1 cut(s)	819
Psp6I	CCWGG	1 cut(s)	1759
PspFI	CCCAGC	1 cut(s)	1160
PspGI	CCWGG	1 cut(s)	1759
PspN4I	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1728
PspPI	GGNCC	1 cut(s)	1530
PstI	CTGCAG	1 cut(s)	1660
PstNI	CAGNNNCTG	1 cut(s)	878
PsuI	RGATCY	1 cut(s)	1270
PsyI	GACNNNGTC	1 cut(s)	1880
RsaI	GTAC	6 cut(s)	435, 505, 517, 1128, 1345, 1834
RsaNI	GTAC	6 cut(s)	434, 504, 516, 1127, 1344, 1833
RseI	CAYNNNNRTG	3 cut(s)	267, 669, 1265
SacI	GAGCTC	1 cut(s)	819
SaqAI	TTAA	6 cut(s)	30, 227, 273, 303, 312, 1584
SatI	GCNGC	8 cut(s)	24, 73, 855, 1047, 1050, 1065, 1193, 1479
Sau3AI	GATC	5 cut(s)	709, 1018, 1270, 1329, 1575
Sau96I	GGNCC	1 cut(s)	1530
ScaI	AGTACT	1 cut(s)	517
SchI	GAGTC	4 cut(s)	82, 951, 1639, 1802
ScrFI	CCNGG	2 cut(s)	10, 1761
SduI	GDGCHC	2 cut(s)	48, 819
SfaNI	GCATC	3 cut(s)	334, 896, 1721
SfcI	CTRYAG	3 cut(s)	138, 1213, 1656
SinI	GGWCC	1 cut(s)	1530
SmiMI	CAYNNNNRTG	3 cut(s)	267, 669, 1265
SmlI	CTYRAG	4 cut(s)	83, 950, 1325, 1647
SmoI	CTYRAG	4 cut(s)	83, 950, 1325, 1647
SseBI	AGGCCT	1 cut(s)	238
SsiI	CCGC	4 cut(s)	24, 769, 1354, 1797
SspI	AATATT	3 cut(s)	730, 1123, 1582
SspMI	CTAG	5 cut(s)	618, 630, 893, 1025, 1232
SstI	GAGCTC	1 cut(s)	819
StuI	AGGCCT	1 cut(s)	238
StyD4I	CCNGG	2 cut(s)	8, 1759
StyI	CCWWGG	2 cut(s)	418, 882
TaaI	ACNGT	5 cut(s)	433, 539, 1343, 1566, 1779
TaqI	TCGA	2 cut(s)	126, 1814
TaqII	GACCGA	2 cut(s)	809, 1894
TatI	WGTACW	4 cut(s)	433, 515, 1126, 1832
TauI	GCSGC	1 cut(s)	26
TfiI	GAWTC	7 cut(s)	278, 361, 589, 904, 1077, 1801, 1901
Tru1I	TTAA	6 cut(s)	30, 227, 273, 303, 312, 1584
Tru9I	TTAA	6 cut(s)	30, 227, 273, 303, 312, 1584
TscAI	CASTG	4 cut(s)	542, 1272, 1531, 1665
TseFI	GTSAC	2 cut(s)	427, 1906
TseI	GCWGC	7 cut(s)	72, 854, 1046, 1049, 1064, 1192, 1478
Tsp45I	GTSAC	2 cut(s)	427, 1906
TspGWI	ACGGA	1 cut(s)	1925
TspRI	CASTG	4 cut(s)	542, 1272, 1531, 1665
Tth111I	GACNNNGTC	1 cut(s)	1880
VpaK11BI	GGWCC	1 cut(s)	1530
XagI	CCTNNNNNAGG	2 cut(s)	422, 1490
XapI	RAATTY	3 cut(s)	230, 934, 1598
XbaI	TCTAGA	1 cut(s)	1231
XceI	RCATGY	4 cut(s)	188, 359, 440, 1133
XcmI	CCANNNNNNNNNTGG	2 cut(s)	915, 1390
XmiI	GTMKAC	1 cut(s)	1682
XspI	CTAG	5 cut(s)	618, 630, 893, 1025, 1232
ZrmI	AGTACT	1 cut(s)	517
Zsp2I	ATGCAT	1 cut(s)	1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.