Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009128

Physical Location & Seq

Genomic Coordinates Forward (+)

9395097 .. 9401946

Gene Structure 6850 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1068200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1935 bp

ATGGCTTCCCGGAAACACTTGGCCGCTGTAAAGGGCGCGAAAGTGCTCATGGTTGGGGCAGGTGGTATCGGCTGCGAGCTTCTCAAGACTCTCGCTCTCTCTGGCTTCCAAGACATTCATATTATTGACATGGACACTATAGAAGTGAGTAACCTCAACAGACAATTCCTCTTCCGACAATCACATGTTGGGCAATCCAAAGCCAAAGTTGCTCGGGAAGCTGTCTTAAAATTTAGACCTGAGATAAGCATAACTTCATACCATGCCAATGTAAAGGATTCTGACTTCAATGTGGACTTCTTTAAGAACTTTAATGTGGTCTTGAATGGACTTGACAACTTAGATGCTAGACGACATGTGAATCGCCTGTGTTTGGCAGCTGATGTTCCCTTGATTGAAAGTGGGACAACTGGGTTCCTTGGACAGGTGACTGTACATGTGAAGGGAAAAACAGAGTGCTATGAATGTCAGCCAAAACCTGCTCCTAAAACTTATCCTGTCTGTACCATTACCAGTACTCCATCAAAGTTTGTTCACTGTATCGTATGGGCAAAGGAACTTCTCTTTGCAAAGTTGTTTGGGGATAAGAATCAGGCAAATGATTTGAATGTACGTTCTAGTGATGGTGCTAGCTCATCAGAACAGGCTGAAGATGTATTTGAACATAGAAATGATGAAGACATTGAGCAGTATGGAAGAAGAATATATGATCATGTATTTGGTTATAACATTGAGCAAGCTTTGTCTAATGAGGAGACATGGAAGAACCGCAATAGACCAAGGCCTATATATAGTAGGGATGTCATGCCTAATGGGCTCGGTCAACAGAATGGAAGTATGGAAAAGAATGGTGCATCCAATGATGTATATTCAGTATCTGCCATGGCATCTCTAGGAATGAAGAATCCACAGGATATATGGAGCCTTATGGAAAATTCCAAAGTCTTTCTTGAGGCTTTGAAACTATTTTTCTTGAAAAGGCAGAAGGAGATTGGAAATATGACTTTTGATAAAGATGATCAGCTAGCTGTAGAATTTGTTACTTCTGCTGCAAATATACGGGCTGCTTCTTTTGGAATCCCATTGCATAGCCTTTTTGAAGCCAAAGGCATTGCTGGTAATATTGTTCATGCTGTTGCAACAACGAATGCTATTATTGCTGGGTTGATTGTCATTGAGGCAATAAAGGTGCTGCAAAATGATACAAAGAATTATAGGATGACATATTGTCTGGAACATCCAGCAAGAAAAATGCTGCTCATGCCAGTGGATCCATTTGAGCCTAACAAGTCCTGCTATGTCTGTTCAGAGACACCACTATCACTTGAGATCAATACTCACTGCACAAAGTTGCGGGATGTAGTTGAAAAGATTGTTAGAGCCAAGCTTGGGATGAGCTTGCCACTCATTATGCATGGGTCAGCCCTTCTTTATGAAGTTGGTGATGACCTGGACGAAGACATGGTTGCAAATTATGCAGCAAACCTTGATAAGGTGCTGTCTGAGCTTCCTACGCCAGTTACCAGTGGGACCATGCTGACAATTGAGGATCTTCAGCAAGAACTATCATGCAAGATCAATATTAAGCACAGAGAGGAGTTTGATGAAGAGAAGGAACCCGATGGAATGGTTTTATCTGGTTGGACTCAATCTCCTGCGGTGGCACAGGATGACAAGAAGTCTACTGCAAATGCTGGAAGCACGTCAAATGCATCATCATCTATGGAGGCTGAAAGGACTGATGATGTTGAAACTACTCCTGGAAGGAAGAGAAAACTGTCTGAGGTTTCTGAGGCAGTCAATCCAGATATACCAAGTGTTGCTGGCAGTAGTACAAGAAATCCCGAGAAATTGGAACTTGTTGATGACGACGATGAGCTTGTCATATTTGATAATTGGGATTCAGTCACCAGCAAGAAGATAAAGTTGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008641 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016874 GO:0016877 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0019948 GO:0022414 GO:0030054 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0061458 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

ThiF UAE_UbL UBA_e1_thiolCys

Protein Analysis

645

Amino Acids

71.39

Weight (kDa)

5.42

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	8 - 432	9.2e-66	ThiF family
UAE_UbL	PF14732	442 - 534	2.8e-22	Ubiquitin/SUMO-activating enzyme ubiquitin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21470 AT2G21470
fragaria_vesca	FvH4_2g09750 FvH4_2g09750 FvH4_2g09750
malus_domestica	MD05G1060400.v1.1 MD10G1068200.v1.1
prunus_persica	Prupe.8G090100_v2.0.a1
pyrus_communis	pycom05g05140 pycom10g05400
rosa_chinensis	RchiOBHm_Chr6g0266041
rosa_laevigata	RLG00000014122
rosa_multiflora	Rmu_sc0000776.1_g000024
rosa_roxburghii	Rroxscaffold_174G00435160 Rroxscaffold_7G00202040
rosa_rugosa	Rorug06G0022900 Rorug06G0022900
rosa_samantha	Rh6AG144100 Rh6BG144600 Rh6CG141100 Rh6DG129000
rosa_wichuraiana	Rw6G012450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	726
AarI	CACCTGC	1 cut(s)	50
Acc36I	ACCTGC	2 cut(s)	50, 487
AccI	GTMKAC	1 cut(s)	1682
AccII	CGCG	1 cut(s)	38
AciI	CCGC	4 cut(s)	24, 769, 1354, 1658
AclWI	GGATC	3 cut(s)	1265, 1278, 1557
AcoI	YGGCCR	1 cut(s)	21
AcsI	RAATTY	3 cut(s)	230, 934, 1034
AcuI	CTGAAG	2 cut(s)	669, 1538
AfaI	GTAC	5 cut(s)	435, 505, 517, 612, 1834
AfiI	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
AflIII	ACRYGT	3 cut(s)	184, 355, 436
AhdI	GACNNNNNGTC	2 cut(s)	1227, 1678
AjiI	CACGTC	1 cut(s)	1704
AjnI	CCWGG	2 cut(s)	1449, 1759
Alw21I	GWGCWC	1 cut(s)	48
Alw26I	GTCTC	2 cut(s)	749, 1304
AlwI	GGATC	3 cut(s)	1265, 1278, 1557
AlwNI	CAGNNNCTG	1 cut(s)	878
Ama87I	CYCGRG	2 cut(s)	213, 1844
AoxI	GGCC	2 cut(s)	21, 782
ApeKI	GCWGC	7 cut(s)	72, 377, 1049, 1064, 1192, 1255, 1478
ApoI	RAATTY	3 cut(s)	230, 934, 1034
AspLEI	GCGC	1 cut(s)	38
AspS9I	GGNCC	1 cut(s)	1530
AsuC2I	CCSGG	1 cut(s)	10
AsuHPI	GGTGA	3 cut(s)	439, 1454, 1900
AsuNHI	GCTAGC	2 cut(s)	629, 1024
AvaI	CYCGRG	2 cut(s)	213, 1844
AvaII	GGWCC	1 cut(s)	1530
BamHI	GGATCC	1 cut(s)	1270
BanII	GRGCYC	1 cut(s)	819
BbsI	GAAGAC	2 cut(s)	684, 1464
Bbv12I	GWGCWC	1 cut(s)	48
BbvI	GCAGC	7 cut(s)	59, 389, 1036, 1051, 1179, 1242, 1490
BccI	CCATC	3 cut(s)	529, 617, 1616
BciT130I	CCWGG	2 cut(s)	1451, 1761
BclI	TGATCA	2 cut(s)	709, 1018
BcnI	CCSGG	1 cut(s)	10
BcoDI	GTCTC	2 cut(s)	749, 1304
BfaI	CTAG	5 cut(s)	348, 618, 630, 893, 1025
BfmI	CTRYAG	2 cut(s)	138, 1029
BfuAI	ACCTGC	2 cut(s)	50, 487
BglI	GCCNNNNNGGC	1 cut(s)	814
BisI	GCNGC	8 cut(s)	24, 73, 378, 1050, 1065, 1193, 1256, 1479
BlsI	GCNGC	8 cut(s)	25, 74, 379, 1051, 1066, 1194, 1257, 1480
BmcAI	AGTACT	1 cut(s)	517
Bme1390I	CCNGG	3 cut(s)	10, 1451, 1761
Bme18I	GGWCC	1 cut(s)	1530
BmeRI	GACNNNNNGTC	2 cut(s)	1227, 1678
BmeT110I	CYCGRG	2 cut(s)	213, 1844
BmgBI	CACGTC	1 cut(s)	1704
BmgT120I	GGNCC	1 cut(s)	1530
BmiI	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1617
BmrFI	CCNGG	3 cut(s)	10, 1451, 1761
BmrI	ACTGGG	1 cut(s)	420
BmsI	GCATC	4 cut(s)	334, 863, 896, 1721
BmtI	GCTAGC	2 cut(s)	633, 1028
BmuI	ACTGGG	1 cut(s)	420
BpiI	GAAGAC	2 cut(s)	684, 1464
BpuEI	CTTGAG	3 cut(s)	68, 971, 1346
BpuMI	CCSGG	1 cut(s)	10
BsaBI	GATNNNNATC	1 cut(s)	588
BsaJI	CCNNGG	3 cut(s)	418, 779, 882
BsaXI	ACNNNNNCTCC	4 cut(s)	502, 532, 1636, 1666
Bsc4I	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
Bse1I	ACTGG	5 cut(s)	415, 513, 1265, 1517, 1524
Bse3DI	GCAATG	2 cut(s)	1082, 1110
Bse8I	GATNNNNATC	1 cut(s)	588
BseBI	CCWGG	2 cut(s)	1451, 1761
BseDI	CCNNGG	3 cut(s)	418, 779, 882
BseGI	GGATG	7 cut(s)	805, 854, 1224, 1237, 1363, 1398, 1675
BseJI	GATNNNNATC	1 cut(s)	588
BseLI	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
BseMI	GCAATG	2 cut(s)	1082, 1110
BseMII	CTCAG	4 cut(s)	231, 1494, 1773, 1782
BseNI	ACTGG	5 cut(s)	415, 513, 1265, 1517, 1524
BseRI	GAGGAG	2 cut(s)	767, 1610
BseXI	GCAGC	7 cut(s)	59, 389, 1036, 1051, 1179, 1242, 1490
BseYI	CCCAGC	1 cut(s)	1160
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	1 cut(s)	38
BshFI	GGCC	2 cut(s)	23, 784
BsiHKAI	GWGCWC	1 cut(s)	48
BsiHKCI	CYCGRG	2 cut(s)	213, 1844
BsiSI	CCGG	1 cut(s)	10
BslFI	GGGAC	2 cut(s)	418, 1543
BslI	CCNNNNNNNGG	4 cut(s)	373, 424, 1389, 1492
BsmAI	GTCTC	2 cut(s)	749, 1304
BsmFI	GGGAC	2 cut(s)	418, 1543
BsmI	GAATGC	1 cut(s)	1153
BsnI	GGCC	2 cut(s)	23, 784
BsoBI	CYCGRG	2 cut(s)	213, 1844
Bsp1286I	GDGCHC	2 cut(s)	48, 819
Bsp1407I	TGTACA	1 cut(s)	433
Bsp143I	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
Bsp19I	CCATGG	1 cut(s)	882
BspACI	CCGC	4 cut(s)	24, 769, 1354, 1658
BspANI	GGCC	2 cut(s)	23, 784
BspCNI	CTCAG	4 cut(s)	232, 1495, 1774, 1783
BspFNI	CGCG	1 cut(s)	38
BspLI	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1617
BspMI	ACCTGC	2 cut(s)	50, 487
BspOI	GCTAGC	2 cut(s)	633, 1028
BspPI	GGATC	3 cut(s)	1265, 1278, 1557
BsrDI	GCAATG	2 cut(s)	1082, 1110
BsrGI	TGTACA	1 cut(s)	433
BsrI	ACTGG	5 cut(s)	415, 513, 1265, 1517, 1524
BssECI	CCNNGG	3 cut(s)	418, 779, 882
BssMI	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
BssT1I	CCWWGG	3 cut(s)	418, 779, 882
Bst2UI	CCWGG	2 cut(s)	1451, 1761
Bst4CI	ACNGT	3 cut(s)	433, 539, 1779
Bst6I	CTCTTC	3 cut(s)	176, 1602, 1763
BstAPI	GCANNNNNTGC	1 cut(s)	1475
BstAUI	TGTACA	1 cut(s)	433
BstC8I	GCNNGC	6 cut(s)	77, 631, 738, 1026, 1400, 1825
BstDEI	CTNAG	5 cut(s)	240, 340, 1503, 1782, 1791
BstDSI	CCRYGG	1 cut(s)	882
BstENI	CCTNNNNNAGG	2 cut(s)	422, 1490
BstF5I	GGATG	7 cut(s)	805, 854, 1224, 1237, 1363, 1398, 1675
BstFNI	CGCG	1 cut(s)	38
BstHHI	GCGC	1 cut(s)	38
BstKTI	GATC	6 cut(s)	712, 1021, 1273, 1332, 1552, 1578
BstMAI	GTCTC	2 cut(s)	749, 1304
BstMBI	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
BstMWI	GCNNNNNNNGC	8 cut(s)	209, 218, 814, 1157, 1261, 1475, 1504, 1513
BstNI	CCWGG	2 cut(s)	1451, 1761
BstNSI	RCATGY	3 cut(s)	188, 359, 440
BstSCI	CCNGG	3 cut(s)	8, 1449, 1759
BstSFI	CTRYAG	2 cut(s)	138, 1029
BstUI	CGCG	1 cut(s)	38
BstV1I	GCAGC	7 cut(s)	59, 389, 1036, 1051, 1179, 1242, 1490
BstV2I	GAAGAC	2 cut(s)	684, 1464
BstX2I	RGATCY	2 cut(s)	1270, 1549
BstYI	RGATCY	2 cut(s)	1270, 1549
BsuRI	GGCC	2 cut(s)	23, 784
BtgI	CCRYGG	1 cut(s)	882
BtrI	CACGTC	1 cut(s)	1704
BtsCI	GGATG	7 cut(s)	805, 854, 1224, 1237, 1363, 1398, 1675
BtsI	GCAGTG	1 cut(s)	1339
BtsIMutI	CAGTG	4 cut(s)	535, 1272, 1339, 1531
BveI	ACCTGC	2 cut(s)	50, 487
Cac8I	GCNNGC	6 cut(s)	77, 631, 738, 1026, 1400, 1825
CaiI	CAGNNNCTG	1 cut(s)	878
CfoI	GCGC	1 cut(s)	38
Cfr13I	GGNCC	1 cut(s)	1530
Csp6I	GTAC	5 cut(s)	434, 504, 516, 611, 1833
CviQI	GTAC	5 cut(s)	434, 504, 516, 611, 1833
DdeI	CTNAG	5 cut(s)	240, 340, 1503, 1782, 1791
DpnI	GATC	6 cut(s)	711, 1020, 1272, 1331, 1551, 1577
DpnII	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
DriI	GACNNNNNGTC	2 cut(s)	1227, 1678
EaeI	YGGCCR	1 cut(s)	21
Eam1104I	CTCTTC	3 cut(s)	176, 1602, 1763
Eam1105I	GACNNNNNGTC	2 cut(s)	1227, 1678
EarI	CTCTTC	3 cut(s)	176, 1602, 1763
Eco130I	CCWWGG	3 cut(s)	418, 779, 882
Eco147I	AGGCCT	1 cut(s)	784
Eco24I	GRGCYC	1 cut(s)	819
Eco47I	GGWCC	1 cut(s)	1530
Eco57I	CTGAAG	2 cut(s)	669, 1538
Eco88I	CYCGRG	2 cut(s)	213, 1844
EcoNI	CCTNNNNNAGG	2 cut(s)	422, 1490
EcoRII	CCWGG	2 cut(s)	1449, 1759
EcoT14I	CCWWGG	3 cut(s)	418, 779, 882
EcoT22I	ATGCAT	2 cut(s)	1416, 1714
EcoT38I	GRGCYC	1 cut(s)	819
ErhI	CCWWGG	3 cut(s)	418, 779, 882
FalI	AAGNNNNNCTT	4 cut(s)	238, 270, 933, 965
FaqI	GGGAC	2 cut(s)	418, 1543
FauI	CCCGC	1 cut(s)	1347
FbaI	TGATCA	2 cut(s)	709, 1018
FblI	GTMKAC	1 cut(s)	1682
Fnu4HI	GCNGC	8 cut(s)	24, 73, 378, 1050, 1065, 1193, 1256, 1479
FokI	GGATG	7 cut(s)	812, 841, 1224, 1231, 1370, 1405, 1682
FriOI	GRGCYC	1 cut(s)	819
Fsp4HI	GCNGC	8 cut(s)	24, 73, 378, 1050, 1065, 1193, 1256, 1479
FspBI	CTAG	5 cut(s)	348, 618, 630, 893, 1025
GlaI	GCGC	1 cut(s)	37
GluI	GCNGC	8 cut(s)	24, 73, 378, 1050, 1065, 1193, 1256, 1479
GsaI	CCCAGC	1 cut(s)	1164
HaeIII	GGCC	2 cut(s)	23, 784
HapII	CCGG	1 cut(s)	10
HhaI	GCGC	1 cut(s)	38
Hin6I	GCGC	1 cut(s)	36
HinP1I	GCGC	1 cut(s)	36
HincII	GTYRAC	1 cut(s)	824
HindII	GTYRAC	1 cut(s)	824
HindIII	AAGCTT	2 cut(s)	738, 1385
HinfI	GANTC	8 cut(s)	88, 278, 361, 589, 904, 1077, 1645, 1901
HpaII	CCGG	1 cut(s)	10
HphI	GGTGA	3 cut(s)	439, 1454, 1900
Hpy166II	GTNNAC	4 cut(s)	295, 535, 824, 1683
Hpy188I	TCNGA	7 cut(s)	176, 283, 640, 1309, 1504, 1783, 1792
Hpy188III	TCNNGA	8 cut(s)	85, 215, 322, 950, 973, 1232, 1805, 1844
Hpy8I	GTNNAC	4 cut(s)	295, 535, 824, 1683
Hpy99I	CGWCG	1 cut(s)	1874
HpyAV	CCTTC	5 cut(s)	436, 979, 1436, 1606, 1758
HpyCH4III	ACNGT	3 cut(s)	433, 539, 1779
HpyCH4IV	ACGT	2 cut(s)	613, 1703
HpyF10VI	GCNNNNNNNGC	8 cut(s)	209, 218, 814, 1157, 1261, 1475, 1504, 1513
HpyF3I	CTNAG	5 cut(s)	240, 340, 1503, 1782, 1791
HpySE526I	ACGT	2 cut(s)	613, 1703
HspAI	GCGC	1 cut(s)	36
Ksp22I	TGATCA	2 cut(s)	709, 1018
Kzo9I	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
LmnI	GCTCC	2 cut(s)	487, 921
Lsp1109I	GCAGC	7 cut(s)	59, 389, 1036, 1051, 1179, 1242, 1490
LweI	GCATC	4 cut(s)	334, 863, 896, 1721
MaeI	CTAG	5 cut(s)	348, 618, 630, 893, 1025
MaeII	ACGT	2 cut(s)	613, 1703
MaeIII	GTNAC	5 cut(s)	149, 427, 1039, 1519, 1906
MalI	GATC	6 cut(s)	711, 1020, 1272, 1331, 1551, 1577
MboI	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
MfeI	CAATTG	1 cut(s)	1542
MflI	RGATCY	2 cut(s)	1270, 1549
MhlI	GDGCHC	2 cut(s)	48, 819
MluCI	AATT	9 cut(s)	164, 230, 934, 1034, 1210, 1471, 1542, 1850, 1894
MlyI	GAGTC	2 cut(s)	82, 1639
MmeI	TCCRAC	2 cut(s)	199, 1622
Mph1103I	ATGCAT	2 cut(s)	1416, 1714
MseI	TTAA	4 cut(s)	227, 303, 312, 1584
MslI	CAYNNNNRTG	3 cut(s)	267, 669, 1265
MspA1I	CMGCKG	2 cut(s)	26, 380
MspI	CCGG	1 cut(s)	10
MspR9I	CCNGG	3 cut(s)	10, 1451, 1761
MunI	CAATTG	1 cut(s)	1542
Mva1269I	GAATGC	1 cut(s)	1153
MvaI	CCWGG	2 cut(s)	1451, 1761
MvnI	CGCG	1 cut(s)	38
MwoI	GCNNNNNNNGC	8 cut(s)	209, 218, 814, 1157, 1261, 1475, 1504, 1513
NciI	CCSGG	1 cut(s)	10
NcoI	CCATGG	1 cut(s)	882
NdeII	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
NheI	GCTAGC	2 cut(s)	629, 1024
NlaIV	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1617
NmuCI	GTSAC	2 cut(s)	427, 1906
NsiI	ATGCAT	2 cut(s)	1416, 1714
NspI	RCATGY	3 cut(s)	188, 359, 440
PaqCI	CACCTGC	1 cut(s)	50
PceI	AGGCCT	1 cut(s)	784
PciI	ACATGT	3 cut(s)	184, 355, 436
PctI	GAATGC	1 cut(s)	1153
PfeI	GAWTC	6 cut(s)	278, 361, 589, 904, 1077, 1901
PfoI	TCCNGGA	2 cut(s)	8, 1759
PkrI	GCNGC	8 cut(s)	25, 74, 379, 1051, 1066, 1194, 1257, 1480
PleI	GAGTC	2 cut(s)	82, 1639
PpsI	GAGTC	2 cut(s)	82, 1639
PscI	ACATGT	3 cut(s)	184, 355, 436
PsiI	TTATAA	1 cut(s)	726
Psp6I	CCWGG	2 cut(s)	1449, 1759
PspFI	CCCAGC	1 cut(s)	1160
PspGI	CCWGG	2 cut(s)	1449, 1759
PspN4I	GGNNCC	5 cut(s)	416, 923, 1272, 1531, 1617
PspPI	GGNCC	1 cut(s)	1530
PstNI	CAGNNNCTG	1 cut(s)	878
PsuI	RGATCY	2 cut(s)	1270, 1549
PvuII	CAGCTG	1 cut(s)	380
RsaI	GTAC	5 cut(s)	435, 505, 517, 612, 1834
RsaNI	GTAC	5 cut(s)	434, 504, 516, 611, 1833
RseI	CAYNNNNRTG	3 cut(s)	267, 669, 1265
SaqAI	TTAA	4 cut(s)	227, 303, 312, 1584
SatI	GCNGC	8 cut(s)	24, 73, 378, 1050, 1065, 1193, 1256, 1479
Sau3AI	GATC	6 cut(s)	709, 1018, 1270, 1329, 1549, 1575
Sau96I	GGNCC	1 cut(s)	1530
ScaI	AGTACT	1 cut(s)	517
SchI	GAGTC	2 cut(s)	82, 1639
ScrFI	CCNGG	3 cut(s)	10, 1451, 1761
SduI	GDGCHC	2 cut(s)	48, 819
SfaNI	GCATC	4 cut(s)	334, 863, 896, 1721
SfcI	CTRYAG	2 cut(s)	138, 1029
SinI	GGWCC	1 cut(s)	1530
SmiMI	CAYNNNNRTG	3 cut(s)	267, 669, 1265
SmlI	CTYRAG	3 cut(s)	83, 950, 1325
SmoI	CTYRAG	3 cut(s)	83, 950, 1325
Sse9I	AATT	9 cut(s)	164, 230, 934, 1034, 1210, 1471, 1542, 1850, 1894
SseBI	AGGCCT	1 cut(s)	784
SsiI	CCGC	4 cut(s)	24, 769, 1354, 1658
SspI	AATATT	2 cut(s)	1123, 1582
SspMI	CTAG	5 cut(s)	348, 618, 630, 893, 1025
StuI	AGGCCT	1 cut(s)	784
StyD4I	CCNGG	3 cut(s)	8, 1449, 1759
StyI	CCWWGG	3 cut(s)	418, 779, 882
TaaI	ACNGT	3 cut(s)	433, 539, 1779
TaiI	ACGT	2 cut(s)	616, 1706
TaqII	GACCGA	1 cut(s)	809
TasI	AATT	9 cut(s)	164, 230, 934, 1034, 1210, 1471, 1542, 1850, 1894
TatI	WGTACW	3 cut(s)	433, 515, 1832
TauI	GCSGC	1 cut(s)	26
TfiI	GAWTC	6 cut(s)	278, 361, 589, 904, 1077, 1901
Tru1I	TTAA	4 cut(s)	227, 303, 312, 1584
Tru9I	TTAA	4 cut(s)	227, 303, 312, 1584
TscAI	CASTG	4 cut(s)	542, 1272, 1346, 1531
TseFI	GTSAC	2 cut(s)	427, 1906
TseI	GCWGC	7 cut(s)	72, 377, 1049, 1064, 1192, 1255, 1478
Tsp45I	GTSAC	2 cut(s)	427, 1906
TspDTI	ATGAA	8 cut(s)	107, 246, 477, 690, 914, 1118, 1449, 1620
TspRI	CASTG	4 cut(s)	542, 1272, 1346, 1531
VpaK11BI	GGWCC	1 cut(s)	1530
XagI	CCTNNNNNAGG	2 cut(s)	422, 1490
XapI	RAATTY	3 cut(s)	230, 934, 1034
XceI	RCATGY	3 cut(s)	188, 359, 440
XcmI	CCANNNNNNNNNTGG	1 cut(s)	915
XmiI	GTMKAC	1 cut(s)	1682
XspI	CTAG	5 cut(s)	348, 618, 630, 893, 1025
ZrmI	AGTACT	1 cut(s)	517
Zsp2I	ATGCAT	2 cut(s)	1416, 1714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.