MD05G1067400.v1.1

Guanine nucleotide-binding protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010337
Physical Location & Seq
Forward (+)
12696086 .. 12701262
5177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1067400.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGTCAGTCGCGGAGCTCAAAGAGCGCCACGTGGCGGCCAGCGACACCGTCAACTCGCTCAGAGAGCGGCTGAAGCAGAAGCGCGCTTCCTTGCTGGACACAGATGTGGCTGAGTATGCAAGGTCACAGGGGAAGACTCCGGTCACATTTGGCCCCACCGATCTCGTATGCTGCAGAACCTTGCAAGGTCATACAGGAAAGGTGTATTCGTTAGATTGGACCTCTGAAAAGAATCGAATTGTCAGTGCATCTCAAGACGGGCGTTTAATAGTGTGGAATGCACTAACAAGCCAGAAGACTCATGCCATAAAACTACCTTGTGCATGGGTCATGACTTGTGCTTTCTCACCGACTGGTCAATCTGTTGCTTGCGGTGGTCTTGATAGTGTTTGCTCTATTTTCAACTTGAATTCCCTGTGTGACAAGGATGGGAATCTACCTGTATCAAGAACACTTAGTGGGCATAAGGGTTATGTTTCCTCCTGTCAATACGTTCCAGATGAGGACACTCACCTGATTAGTGGATCTGGAGATCAAACGTGTGTTTTATGGGATATTACTACTGGACTCAGGACTTCAGTTTTTGGAGGTGAATTTCAGTCTGGACACACTGCAGATGTACTGAGTGTGTCCATCAATCAAAGCAACTCGAGATTGTTTGTGTCTGGTTCTTGTGATACAACTGCCCGATTGTGGGATACTCGTGTTGCAAGTCGAGCAGTGCGGACGTTTCATGGTCATGAGGGGGATGTTAATGTGGTAAAGTTCTTTCCAGATGGAAATAGATTTGGAACTGGCTCAGATGATGGAACTTGCAGATTGTTTGACATTAGAACCGGGCACCAGCTCCAAGTATACCATCAGCCACATGGTGATAATGATATCCCACCTGTGAAAACCATTGCATTCTCAATATCAGGAAGGCTTCTCTTTGCTGGATACACAAATGGGGATTGCTATGTATGGGATACTTTGTTGGCAAAGGTGGTTTTGAACCTGGGATCCCTCCAAAACTCACACGAGGCACAGATTAGCTGTTTGGGTTTGTCAGCTGATGGTAGTGCCTTGTGTACAGGAAGTTGGGATACAAATTTAAAGATATGGGCCTTCGGAGGGCATAGGAAAATAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009753 GO:0009755 GO:0009787 GO:0009788 GO:0009791 GO:0009814 GO:0009817 GO:0009845 GO:0009867 GO:0009898 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010033 GO:0010118 GO:0010154 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019897 GO:0019898 GO:0022414 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0030968 GO:0031234 GO:0031461 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045087 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071944 GO:0072593 GO:0080008 GO:0090351 GO:0090696 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:0099402 GO:1901419 GO:1901420 GO:1901700 GO:1901701 GO:1902494 GO:1905360 GO:1905392 GO:1905957 GO:1905958 GO:1990234 GO:2000026 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04011 ko04014 ko04062 ko04151 ko04371 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04740 ko04744 ko04926 ko05032 ko05034 ko05167 ko05200 map04011 map04014 map04062 map04151 map04371 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04740 map04744 map04926 map05032 map05034 map05167 map05200
Pfam Domains
Protein Families
WD40

Protein Analysis

378

Amino Acids

40.91

Weight (kDa)

6.87

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 19 - 368 2.8e-101 G protein beta WD-40 repeat protein
Beta-prop_WDR35_TULP_N PF24797 42 - 95 9e-06 WDR35/TULP4 N-terminal
Beta-prop_WDR5 PF25175 55 - 136 3.6e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 56 - 190 6.4e-14 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 56 - 138 8.8e-11 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 56 - 93 2.6e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 57 - 137 1.5e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 58 - 138 8.8e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 59 - 138 2.6e-12 THOC3 beta-propeller domain
EIF3I PF24805 60 - 123 1.7e-06 EIF3I
Beta-prop_EML_2 PF23414 67 - 191 1.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 68 - 254 5.2e-16 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 70 - 188 7.4e-06 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 78 - 226 3.4e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 89 - 189 7.3e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 109 - 193 1.1e-11 THOC3 beta-propeller domain
WDR55 PF24796 110 - 249 7.8e-06 WDR55
WD40_Prp19 PF24814 148 - 323 3.1e-22 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 148 - 330 6.9e-30 WDR5 beta-propeller domain
WD40 PF00400 150 - 185 1.2e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 201 - 285 7.4e-09 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 201 - 369 2.5e-22 Prp19 WD40 domain
WD40 PF00400 201 - 233 4.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 201 - 288 2.4e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 201 - 369 2.9e-24 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 202 - 304 1.3e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 204 - 370 1.1e-14 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 218 - 324 1.5e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 223 - 370 1e-10 WDR36/Utp21 first beta-propeller
WD40 PF00400 241 - 276 1.4e-08 WD domain, G-beta repeat
WDR55 PF24796 241 - 369 7.7e-08 WDR55
EIF3I PF24805 242 - 312 6.5e-06 EIF3I
WD40_CDC20-Fz PF24807 254 - 370 2.5e-09 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 257 - 370 3.6e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 297 - 370 5.1e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34460 AT4G34460 AT4G34460 AT4G34460
fragaria_vesca FvH4_2g08680 FvH4_2g08680
malus_domestica MD05G1067400.v1.1 MD10G1077300.v1.1
prunus_persica Prupe.8G098100_v2.0.a1
pyrus_communis pycom05g05850 pycom10g06030
rosa_chinensis RchiOBHm_Chr6g0264111
rosa_laevigata RLG00000014290
rosa_multiflora Rmu_sc0002305.1_g000002
rosa_roxburghii Rroxscaffold_176G00431420 Rroxscaffold_7G00203590
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG129200 Rh6CG124200 Rh6DG111100
rosa_wichuraiana Rw6G011170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 840
AccBSI CCGCTC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 855
AccII CGCG 2 cut(s) 11, 84
AciI CCGC 5 cut(s) 11, 35, 67, 372, 724
AclWI GGATC 3 cut(s) 532, 996, 1009
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 3 cut(s) 409, 593, 1090
AcuI CTGAAG 2 cut(s) 92, 561
AcvI CACGTG 1 cut(s) 31
AdeI CACNNNGTG 2 cut(s) 458, 872
AfaI GTAC 2 cut(s) 621, 1072
AfiI CCNNNNNNNGG 4 cut(s) 34, 693, 694, 1113
AflIII ACRYGT 1 cut(s) 539
AgsI TTSAA 3 cut(s) 403, 409, 994
AjnI CCWGG 1 cut(s) 996
AleI CACNNNNGTG 1 cut(s) 104
AloI GAACNNNNNNTCC 2 cut(s) 986, 1018
AluBI AGCT 4 cut(s) 16, 847, 1035, 1052
AluI AGCT 4 cut(s) 16, 847, 1035, 1052
Alw21I GWGCWC 1 cut(s) 18
AlwI GGATC 3 cut(s) 532, 996, 1009
Ama87I CYCGRG 1 cut(s) 649
AoxI GGCC 3 cut(s) 36, 151, 1104
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 3 cut(s) 409, 593, 1090
AspLEI GCGC 3 cut(s) 27, 84, 86
AspS9I GGNCC 3 cut(s) 152, 219, 1104
AsuC2I CCSGG 1 cut(s) 838
AsuHPI GGTGA 4 cut(s) 339, 503, 602, 884
AvaI CYCGRG 1 cut(s) 649
AvaII GGWCC 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 843
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
BamHI GGATCC 1 cut(s) 1001
BanI GGYRCC 1 cut(s) 840
BanII GRGCYC 1 cut(s) 18
BarI GAAGNNNNNNTAC 2 cut(s) 1069, 1101
BauI CACGAG 2 cut(s) 702, 1019
BbrPI CACGTG 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 140, 302
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 158
BccI CCATC 6 cut(s) 422, 641, 770, 800, 867, 1049
BciT130I CCWGG 1 cut(s) 998
BciVI GTATCC 4 cut(s) 691, 932, 961, 1078
BcnI CCSGG 1 cut(s) 838
BfmI CTRYAG 2 cut(s) 172, 612
BfoI RGCGCY 1 cut(s) 28
BfuI GTATCC 4 cut(s) 691, 932, 961, 1078
BisI GCNGC 3 cut(s) 36, 68, 172
BlsI GCNGC 3 cut(s) 37, 69, 173
Bme1390I CCNGG 2 cut(s) 838, 998
Bme18I GGWCC 1 cut(s) 219
BmeT110I CYCGRG 1 cut(s) 649
BmgT120I GGNCC 3 cut(s) 152, 219, 1104
BmiI GGNNCC 3 cut(s) 154, 842, 1003
BmrFI CCNGG 2 cut(s) 838, 998
BmsI GCATC 1 cut(s) 257
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 140, 302
BpmI CTGGAG 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 838
BsaAI YACGTR 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 997
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 693, 694, 1113
Bse1I ACTGG 3 cut(s) 358, 568, 799
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 1 cut(s) 998
BseDI CCNNGG 1 cut(s) 997
BseGI GGATG 2 cut(s) 433, 754
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 4 cut(s) 34, 693, 694, 1113
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 5 cut(s) 73, 102, 583, 614, 813
BseNI ACTGG 3 cut(s) 358, 568, 799
BsePI GCGCGC 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 1 cut(s) 158
Bsh1236I CGCG 2 cut(s) 11, 84
BshFI GGCC 3 cut(s) 38, 153, 1106
BshNI GGYRCC 1 cut(s) 840
BsiHKAI GWGCWC 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 649
BsiSI CCGG 2 cut(s) 140, 837
BslI CCNNNNNNNGG 4 cut(s) 34, 693, 694, 1113
BsmI GAATGC 2 cut(s) 283, 905
BsnI GGCC 3 cut(s) 38, 153, 1106
BsoBI CYCGRG 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 18, 843
Bsp1407I TGTACA 1 cut(s) 1070
Bsp143I GATC 4 cut(s) 160, 524, 532, 1001
BspACI CCGC 5 cut(s) 11, 35, 67, 372, 724
BspANI GGCC 3 cut(s) 38, 153, 1106
BspCNI CTCAG 5 cut(s) 72, 103, 582, 615, 812
BspFNI CGCG 2 cut(s) 11, 84
BspHI TCATGA 2 cut(s) 330, 739
BspLI GGNNCC 3 cut(s) 154, 842, 1003
BspMAI CTGCAG 2 cut(s) 176, 616
BspPI GGATC 3 cut(s) 532, 996, 1009
BspT107I GGYRCC 1 cut(s) 840
BsrBI CCGCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 900
BsrGI TGTACA 1 cut(s) 1070
BsrI ACTGG 3 cut(s) 358, 568, 799
BssECI CCNNGG 1 cut(s) 997
BssHII GCGCGC 1 cut(s) 82
BssMI GATC 4 cut(s) 160, 524, 532, 1001
BssNAI GTATAC 1 cut(s) 856
BssSI CACGAG 2 cut(s) 702, 1019
Bst1107I GTATAC 1 cut(s) 856
Bst2BI CACGAG 2 cut(s) 702, 1019
Bst2UI CCWGG 1 cut(s) 998
Bst4CI ACNGT 1 cut(s) 49
BstAUI TGTACA 1 cut(s) 1070
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 3 cut(s) 40, 84, 370
BstDEI CTNAG 6 cut(s) 59, 111, 455, 569, 623, 799
BstF5I GGATG 2 cut(s) 433, 754
BstFNI CGCG 2 cut(s) 11, 84
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 3 cut(s) 27, 84, 86
BstKTI GATC 4 cut(s) 163, 527, 535, 1004
BstMBI GATC 4 cut(s) 160, 524, 532, 1001
BstMWI GCNNNNNNNGC 5 cut(s) 22, 64, 73, 116, 716
BstNI CCWGG 1 cut(s) 998
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 836, 996
BstSFI CTRYAG 2 cut(s) 172, 612
BstSLI GKGCMC 1 cut(s) 843
BstUI CGCG 2 cut(s) 11, 84
BstV1I GCAGC 1 cut(s) 158
BstV2I GAAGAC 2 cut(s) 140, 302
BstX2I RGATCY 2 cut(s) 524, 1001
BstYI RGATCY 2 cut(s) 524, 1001
BstZ17I GTATAC 1 cut(s) 856
BsuI GTATCC 4 cut(s) 691, 932, 961, 1078
BsuRI GGCC 3 cut(s) 38, 153, 1106
BtsCI GGATG 2 cut(s) 433, 754
BtsI GCAGTG 2 cut(s) 609, 726
BtsIMutI CAGTG 3 cut(s) 250, 609, 726
Cac8I GCNNGC 3 cut(s) 40, 84, 370
CciI TCATGA 2 cut(s) 330, 739
CfoI GCGC 3 cut(s) 27, 84, 86
Cfr13I GGNCC 3 cut(s) 152, 219, 1104
Csp6I GTAC 2 cut(s) 620, 1071
CviAII CATG 6 cut(s) 302, 324, 331, 734, 740, 869
CviQI GTAC 2 cut(s) 620, 1071
DdeI CTNAG 6 cut(s) 59, 111, 455, 569, 623, 799
DpnI GATC 4 cut(s) 162, 526, 534, 1003
DpnII GATC 4 cut(s) 160, 524, 532, 1001
DraI TTTAAA 1 cut(s) 1095
DraIII CACNNNGTG 2 cut(s) 458, 872
EaeI YGGCCR 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 18
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 2 cut(s) 92, 561
Eco72I CACGTG 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 996
EcoRV GATATC 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 18
FaeI CATG 6 cut(s) 305, 327, 334, 737, 743, 872
FatI CATG 6 cut(s) 301, 323, 330, 733, 739, 868
FblI GTMKAC 1 cut(s) 855
Fnu4HI GCNGC 3 cut(s) 36, 68, 172
FokI GGATG 2 cut(s) 440, 761
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 3 cut(s) 36, 68, 172
GlaI GCGC 3 cut(s) 26, 83, 85
GluI GCNGC 3 cut(s) 36, 68, 172
GsuI CTGGAG 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 3 cut(s) 38, 153, 1106
HapII CCGG 2 cut(s) 140, 837
HhaI GCGC 3 cut(s) 27, 84, 86
Hin1II CATG 6 cut(s) 305, 327, 334, 737, 743, 872
Hin6I GCGC 3 cut(s) 25, 82, 84
HinP1I GCGC 3 cut(s) 25, 82, 84
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 5 cut(s) 136, 232, 298, 433, 567
HpaII CCGG 2 cut(s) 140, 837
HphI GGTGA 4 cut(s) 339, 503, 602, 884
Hpy166II GTNNAC 3 cut(s) 52, 856, 1071
Hpy188I TCNGA 4 cut(s) 62, 226, 802, 1112
Hpy8I GTNNAC 3 cut(s) 52, 856, 1071
HpyAV CCTTC 2 cut(s) 915, 1117
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 4 cut(s) 30, 492, 539, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 64, 73, 116, 716
HpyF3I CTNAG 6 cut(s) 59, 111, 455, 569, 623, 799
HpySE526I ACGT 4 cut(s) 30, 492, 539, 728
Hsp92II CATG 6 cut(s) 305, 327, 334, 737, 743, 872
HspAI GCGC 3 cut(s) 25, 82, 84
Kzo9I GATC 4 cut(s) 160, 524, 532, 1001
LmnI GCTCC 2 cut(s) 13, 852
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 1 cut(s) 257
MaeII ACGT 4 cut(s) 30, 492, 539, 728
MaeIII GTNAC 3 cut(s) 123, 142, 419
MalI GATC 4 cut(s) 162, 526, 534, 1003
MbiI CCGCTC 1 cut(s) 67
MboI GATC 4 cut(s) 160, 524, 532, 1001
MboII GAAGA 2 cut(s) 145, 307
MflI RGATCY 2 cut(s) 524, 1001
MhlI GDGCHC 2 cut(s) 18, 843
MluCI AATT 5 cut(s) 237, 409, 593, 1090, 1128
MlyI GAGTC 3 cut(s) 130, 292, 561
MnlI CCTC 8 cut(s) 232, 490, 496, 581, 736, 1015, 1016, 1106
MseI TTAA 3 cut(s) 266, 753, 1094
MslI CAYNNNNRTG 2 cut(s) 104, 738
MspA1I CMGCKG 1 cut(s) 1052
MspI CCGG 2 cut(s) 140, 837
MspR9I CCNGG 2 cut(s) 838, 998
Mva1269I GAATGC 2 cut(s) 283, 905
MvaI CCWGG 1 cut(s) 998
MvnI CGCG 2 cut(s) 11, 84
MwoI GCNNNNNNNGC 5 cut(s) 22, 64, 73, 116, 716
NciI CCSGG 1 cut(s) 838
NdeII GATC 4 cut(s) 160, 524, 532, 1001
NlaIII CATG 6 cut(s) 305, 327, 334, 737, 743, 872
NlaIV GGNNCC 3 cut(s) 154, 842, 1003
NmuCI GTSAC 3 cut(s) 123, 142, 419
OliI CACNNNNGTG 1 cut(s) 104
PaeR7I CTCGAG 1 cut(s) 649
PagI TCATGA 2 cut(s) 330, 739
PauI GCGCGC 1 cut(s) 82
PctI GAATGC 2 cut(s) 283, 905
PfeI GAWTC 2 cut(s) 232, 433
PflFI GACNNNGTC 1 cut(s) 47
PkrI GCNGC 3 cut(s) 37, 69, 173
PleI GAGTC 3 cut(s) 130, 292, 561
PmaCI CACGTG 1 cut(s) 31
PmlI CACGTG 1 cut(s) 31
PpsI GAGTC 3 cut(s) 130, 292, 561
Ppu21I YACGTR 1 cut(s) 31
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 996
PspCI CACGTG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 996
PspN4I GGNNCC 3 cut(s) 154, 842, 1003
PspPI GGNCC 3 cut(s) 152, 219, 1104
PstI CTGCAG 2 cut(s) 176, 616
PsuI RGATCY 2 cut(s) 524, 1001
PsyI GACNNNGTC 1 cut(s) 47
PteI GCGCGC 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 2 cut(s) 621, 1072
RsaNI GTAC 2 cut(s) 620, 1071
RseI CAYNNNNRTG 2 cut(s) 104, 738
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 3 cut(s) 266, 753, 1094
SatI GCNGC 3 cut(s) 36, 68, 172
Sau3AI GATC 4 cut(s) 160, 524, 532, 1001
Sau96I GGNCC 3 cut(s) 152, 219, 1104
SchI GAGTC 3 cut(s) 130, 292, 561
ScrFI CCNGG 2 cut(s) 838, 998
SduI GDGCHC 2 cut(s) 18, 843
SfaNI GCATC 1 cut(s) 257
SfcI CTRYAG 2 cut(s) 172, 612
Sfr274I CTCGAG 1 cut(s) 649
SinI GGWCC 1 cut(s) 219
SlaI CTCGAG 1 cut(s) 649
SmiMI CAYNNNNRTG 2 cut(s) 104, 738
SmlI CTYRAG 2 cut(s) 252, 649
SmoI CTYRAG 2 cut(s) 252, 649
Sse9I AATT 5 cut(s) 237, 409, 593, 1090, 1128
SsiI CCGC 5 cut(s) 11, 35, 67, 372, 724
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 2 cut(s) 836, 996
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 4 cut(s) 33, 495, 542, 731
TaqI TCGA 3 cut(s) 235, 650, 715
TasI AATT 5 cut(s) 237, 409, 593, 1090, 1128
TatI WGTACW 2 cut(s) 619, 1070
TauI GCSGC 2 cut(s) 38, 70
TfiI GAWTC 2 cut(s) 232, 433
Tru1I TTAA 3 cut(s) 266, 753, 1094
Tru9I TTAA 3 cut(s) 266, 753, 1094
TscAI CASTG 3 cut(s) 250, 616, 726
TseFI GTSAC 3 cut(s) 123, 142, 419
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 3 cut(s) 123, 142, 419
TspDTI ATGAA 1 cut(s) 722
TspRI CASTG 3 cut(s) 250, 616, 726
Tth111I GACNNNGTC 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 219
XapI RAATTY 3 cut(s) 409, 593, 1090
XcmI CCANNNNNNNNNTGG 1 cut(s) 866
XhoI CTCGAG 1 cut(s) 649
XmiI GTMKAC 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.