MD05G1124400.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014658
Physical Location & Seq
Forward (+)
24657551 .. 24661781
4231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1124400.v1.1.491

Sequence Viewer

Length: 2220 bp
ATGGGGGGATGTGTTGCTTCCAAGCTGGAGGAAGAAGGAGGAGTTGTGACAATTTGTAGAGAGAGAAAACGGCAGCTGAAGCTGGCGGTGGAGAAAAGATACGCCCTTGCGGAGGCGCACTGTAAGTACGGCCATGCTTTGTTTGCGGTGGCTGCCGCCGTGGACTTCTTTGTTGCAGGCTATTCTTCCCCTCCTAAACCTTACCTCATCACATTTTCGCCTCACTCTCCTCCTCCTCAGCCGCCGGAGTCGGCGGCTCCGCTTCCGATCACTGAGAATGTGATAACCAACCCAATGTTTCTTCAGCAGAGACCGTCTGAGTCGACCCATGAAGCCATTGCTTGTCACTCATGTGGTTCTTCAACATCCTCCGACTCCTCTGAGGAGGAGAGAGAAGAAGAGGAGCAAAAAACAGACGTGAGAGAAGAAGAACAGGGATGTGGGTACTTTTATATGCAAATGCCACCAGCAATGCCGTCACCACAGAGAGACTTTGGGTGGGATTTCTTCAACCCTTTCGACGCCGTGAGGCCGGAGGTCATAAGCGGGTACAGGCGGAGCTCCGACGAGGATTTAAGGGTGGTGAGGGAGGAGGAAGGGATACCTGAGCTGGAAGAGGAAGAAGGGGAGAGTCAGAGAGAAGAAGAGCAGGAAGAAAACCAGGCGGTGGTGGTGGAAGAAAAAGGCAGCGGAGAACAGCGCGACGGCGGAGCTGAGGTGGTGAAGGTGGTTGATGAGGCGGCTAATGCGAGGGAGGGGGAGCAAAAAGGGCTTGCTGTGATTGATACACCAGCTGAAGGGAGGGAGCTTCTGGAAGCACTGAAGGACATTGTGGACCTTTTCTCTAGGGCTTCTGATTCTGCAAAGGATGTGTCCAGGATGCTTGATGCTAATAAATTGCAAATGCAGTCTGGTTTGGAGGAAATTAAAGAGAACTCAACAAAGCTAATTCAAGCTATAACATGGCATAACTCAACTTCATCGTCTAAGCTTTCGTCGTGCAAGAGTCTCGTGGCATCCAGATCTAAGGGTTCGTCAACATGGACGGAATTTAACAATGATCTTTTCAATGATTATGGAGGAATGGATTCAGGAAGTCATTCACTTACTCTAGAAAGGCTATATGCCTGGGAAAAGAAGCTCTATGAAGAAGTTAAGGCCGGAGATATGACAAGGAAACTGTATGAGAAAAAATGCTCGCGACTTAAACACCACGACATAGGAGACAATGAGACCATGGACAAAACTCGAGCTGCAGTGAAGGATTTATATGCCAGGATCTTAATTGCAATTCGGAGTGCCGAGTCAATTTCCAAGAGAATCCAGATACTGAGAGATGAAGAACTGCAGCCTCAAATTGTTGAGCTGTTGAAAGGCTTAATGCGCACCTGGAAAATTATGTTGGAATCTCATGAAACCCAACACAAGATCCTTTCTGAGGTGAAATCTTTTGCCAGCTCTGCATATGGAAAGTTCAGCAACATCTCTCACATGCGTGCAACACTTCAGCTCCTCGCTGAGCTTCAGAATTGGCATGCATGCTTTAAGGAGTACGTCGCTGCACAAAAAGCATATGTTGGAACTCTCCATGGTTGGCTAACCAAGTTCGTGGTCCCTGAAGAAAACTTCTACTCGGGGAGCAGGAGTTCAGCTGCGCCGTTTGGCGTCAGTGGCCCCCCGTTGCTCATTATCTGCCATGATTGGTTATCTTCCCTGGAGAAATTACCTGATACTTTGGTGACCATTGCCTTGAGAAGCTTTACAAAGGACGTGAGGGCTCTGTTGAATCAGCAAGGGGAAGAGCAGAAACAAAAAAGGAAAGTTGACAGCCTAGGCAAAGAGCTCGACCGAAGAGTTTGTGCATTCCAGAAGACAGAGAGCAGGTGTCTGGAGCCAAAGCTCACAGAACAGAAATGCGAGACAGATATGGAACATAAGACGGAGTCCATGACAGAGAAGAAGGAGCAGATAGACATGTTCAGAAGAAAGGTGGACATGGAGAGGGAAAAACACCAAAATTACATTGAGGAAACTCAAAGGATCACATTGAACGGAATCCAGACTGGGTTTTGTTCAGTTTTCGAGTCCTTAGCGGAGTTTTCCAGGGCTTCACAGAAACTGTACAGCGATCTCGTGACTTGCAGTGAAAATGCAGATAAAGCTGGGAACCCCGCGTATATAGAAGGCGGCATCAAGGATGACAAAAACAGCAGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

740

Amino Acids

83.34

Weight (kDa)

5.29

Isoelectric Point (pI)

57.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 3 - 59 1.1e-16 Protein of unknown function (DUF630)
DUF632 PF04782 270 - 592 4.2e-105 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014658)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15580
malus_domestica MD05G1124400.v1.1 MD10G1127200.v1.1
prunus_persica Prupe.8G168100_v2.0.a1
pyrus_communis pycom05g11840
rosa_chinensis RchiOBHm_Chr6g0278761
rosa_laevigata RLG00000013195
rosa_multiflora Rmu_sc0004380.1_g000001 Rmu_sc0027143.1_g000002
rosa_roxburghii Rroxscaffold_7G00189650 Rroxscaffold_7G00189920
rosa_rugosa Rorug06G0118700.1
rosa_samantha Rh6AG227500 Rh6BG233100 Rh6CG235300 Rh6DG226800
rosa_wichuraiana Rw6G019960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1872, 2205
Acc16I TGCGCA 1 cut(s) 1385
Acc36I ACCTGC 2 cut(s) 1872, 2205
AccB7I CCANNNNNTGG 1 cut(s) 667
AccI GTMKAC 1 cut(s) 323
AccII CGCG 3 cut(s) 702, 1201, 2174
AclWI GGATC 3 cut(s) 1286, 1423, 2048
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 1 cut(s) 1049
AcuI CTGAAG 7 cut(s) 98, 287, 816, 842, 1490, 1508, 1638
AcyI GRCGYC 2 cut(s) 522, 1665
AfaI GTAC 5 cut(s) 128, 446, 551, 1553, 2123
AfiI CCNNNNNNNGG 4 cut(s) 112, 667, 797, 1438
AflIII ACRYGT 1 cut(s) 1974
AgsI TTSAA 7 cut(s) 363, 511, 953, 1069, 1372, 1786, 2050
AjiI CACGTC 2 cut(s) 418, 1771
AjnI CCWGG 7 cut(s) 660, 875, 1127, 1274, 1388, 1713, 2102
AjuI GAANNNNNNNTTGG 2 cut(s) 1385, 1417
AleI CACNNNNGTG 2 cut(s) 351, 1494
AloI GAACNNNNNNTCC 2 cut(s) 1630, 1662
Alw21I GWGCWC 2 cut(s) 563, 1845
Alw26I GTCTC 6 cut(s) 304, 483, 1013, 1218, 1226, 1913
AlwI GGATC 3 cut(s) 1286, 1423, 2048
AlwNI CAGNNNCTG 2 cut(s) 1330, 2119
Ama87I CYCGRG 2 cut(s) 1248, 1633
AoxI GGCC 4 cut(s) 130, 530, 1158, 1672
ApeKI GCWGC 8 cut(s) 73, 152, 687, 1253, 1348, 1559, 1652, 2211
ApoI RAATTY 1 cut(s) 1049
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
Asp700I GAANNNNTTC 2 cut(s) 1087, 1099
AspA2I CCTAGG 1 cut(s) 1831
AspLEI GCGC 4 cut(s) 118, 702, 1386, 1657
AspS9I GGNCC 3 cut(s) 835, 1612, 1673
AsuHPI GGTGA 5 cut(s) 471, 595, 733, 1453, 1750
AvaI CYCGRG 2 cut(s) 1248, 1633
AvaII GGWCC 2 cut(s) 835, 1612
AvrII CCTAGG 1 cut(s) 1831
BaeI ACNNNNGTAYC 2 cut(s) 1722, 1755
BanII GRGCYC 3 cut(s) 563, 1780, 1845
BauI CACGAG 2 cut(s) 1010, 2132
BbsI GAAGAC 1 cut(s) 1877
Bbv12I GWGCWC 2 cut(s) 563, 1845
BbvCI CCTCAGC 2 cut(s) 237, 714
BbvI GCAGC 7 cut(s) 85, 139, 699, 1240, 1360, 1546, 1639
BceAI ACGGC 7 cut(s) 86, 143, 145, 460, 509, 721, 1642
BcgI CGANNNNNNTGC 4 cut(s) 991, 1025, 1825, 1859
BciT130I CCWGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
BciVI GTATCC 1 cut(s) 594
BcoDI GTCTC 6 cut(s) 304, 483, 1013, 1218, 1226, 1913
BfaI CTAG 3 cut(s) 846, 1112, 1832
BfmI CTRYAG 2 cut(s) 1254, 1346
BfuAI ACCTGC 2 cut(s) 1872, 2205
BfuI GTATCC 1 cut(s) 594
BglII AGATCT 1 cut(s) 1022
BlnI CCTAGG 1 cut(s) 1831
BlpI GCTNAGC 1 cut(s) 1518
Bme1390I CCNGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
Bme18I GGWCC 2 cut(s) 835, 1612
BmeT110I CYCGRG 2 cut(s) 1248, 1633
BmgBI CACGTC 2 cut(s) 418, 1771
BmgT120I GGNCC 3 cut(s) 835, 1612, 1673
BmiI GGNNCC 5 cut(s) 258, 1614, 1675, 1893, 2168
BmrFI CCNGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
BmrI ACTGGG 1 cut(s) 2073
BmsI GCATC 4 cut(s) 870, 877, 1025, 2199
BmuI ACTGGG 1 cut(s) 2073
BpiI GAAGAC 1 cut(s) 1877
BpmI CTGGAG 3 cut(s) 47, 1736, 1910
Bpu10I CCTNAGC 4 cut(s) 237, 606, 714, 2089
Bpu1102I GCTNAGC 1 cut(s) 1518
BpuEI CTTGAG 1 cut(s) 1771
BsaHI GRCGYC 2 cut(s) 522, 1665
BsaI GGTCTC 2 cut(s) 304, 1226
BsaJI CCNNGG 7 cut(s) 159, 1128, 1236, 1588, 1713, 1831, 2103
BsaXI ACNNNNNCTCC 8 cut(s) 30, 60, 702, 732, 1494, 1524, 1630, 1660
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 667, 797, 1438
Bse1I ACTGG 1 cut(s) 2068
Bse3DI GCAATG 3 cut(s) 336, 477, 1743
BseBI CCWGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
BseDI CCNNGG 7 cut(s) 159, 1128, 1236, 1588, 1713, 1831, 2103
BseGI GGATG 7 cut(s) 14, 365, 443, 874, 885, 1016, 2203
BseLI CCNNNNNNNGG 4 cut(s) 112, 667, 797, 1438
BseMI GCAATG 3 cut(s) 336, 477, 1743
BseMII CTCAG 9 cut(s) 251, 264, 309, 372, 597, 705, 1322, 1428, 1509
BseNI ACTGG 1 cut(s) 2068
BseXI GCAGC 7 cut(s) 85, 139, 699, 1240, 1360, 1546, 1639
BseYI CCCAGC 1 cut(s) 2162
BsgI GTGCAG 1 cut(s) 1545
Bsh1236I CGCG 3 cut(s) 702, 1201, 2174
Bsh1285I CGRYCG 1 cut(s) 1849
BshFI GGCC 4 cut(s) 132, 532, 1160, 1674
BsiEI CGRYCG 1 cut(s) 1849
BsiHKAI GWGCWC 2 cut(s) 563, 1845
BsiHKCI CYCGRG 2 cut(s) 1248, 1633
BsiSI CCGG 3 cut(s) 245, 533, 1161
BslFI GGGAC 1 cut(s) 1598
BslI CCNNNNNNNGG 4 cut(s) 112, 667, 797, 1438
BsmAI GTCTC 6 cut(s) 304, 483, 1013, 1218, 1226, 1913
BsmFI GGGAC 1 cut(s) 1598
BsmI GAATGC 1 cut(s) 1862
BsnI GGCC 4 cut(s) 132, 532, 1160, 1674
Bso31I GGTCTC 2 cut(s) 304, 1226
BsoBI CYCGRG 2 cut(s) 1248, 1633
Bsp1286I GDGCHC 3 cut(s) 563, 1780, 1845
Bsp1407I TGTACA 1 cut(s) 2121
Bsp143I GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
Bsp1720I GCTNAGC 1 cut(s) 1518
Bsp19I CCATGG 2 cut(s) 1236, 1588
Bsp68I TCGCGA 1 cut(s) 1201
BspANI GGCC 4 cut(s) 132, 532, 1160, 1674
BspCNI CTCAG 9 cut(s) 250, 265, 310, 373, 598, 706, 1323, 1429, 1510
BspFNI CGCG 3 cut(s) 702, 1201, 2174
BspHI TCATGA 1 cut(s) 1411
BspLI GGNNCC 5 cut(s) 258, 1614, 1675, 1893, 2168
BspMAI CTGCAG 2 cut(s) 1258, 1350
BspMI ACCTGC 2 cut(s) 1872, 2205
BspPI GGATC 3 cut(s) 1286, 1423, 2048
BspQI GCTCTTC 2 cut(s) 639, 1794
BspTNI GGTCTC 2 cut(s) 304, 1226
BsrDI GCAATG 3 cut(s) 336, 477, 1743
BsrGI TGTACA 1 cut(s) 2121
BsrI ACTGG 1 cut(s) 2068
BssECI CCNNGG 7 cut(s) 159, 1128, 1236, 1588, 1713, 1831, 2103
BssMI GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
BssNI GRCGYC 2 cut(s) 522, 1665
BssSI CACGAG 2 cut(s) 1010, 2132
BssT1I CCWWGG 3 cut(s) 1236, 1588, 1831
Bst2BI CACGAG 2 cut(s) 1010, 2132
Bst2UI CCWGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
Bst4CI ACNGT 4 cut(s) 122, 315, 1182, 2121
Bst6I CTCTTC 5 cut(s) 393, 609, 639, 1794, 1846
BstACI GRCGYC 2 cut(s) 522, 1665
BstAUI TGTACA 1 cut(s) 2121
BstC8I GCNNGC 8 cut(s) 84, 178, 774, 1199, 1456, 1497, 1536, 1540
BstDSI CCRYGG 3 cut(s) 159, 1236, 1588
BstEII GGTNACC 1 cut(s) 1738
BstENI CCTNNNNNAGG 2 cut(s) 110, 1436
BstF5I GGATG 7 cut(s) 14, 365, 443, 874, 885, 1016, 2203
BstFNI CGCG 3 cut(s) 702, 1201, 2174
BstHHI GCGC 4 cut(s) 118, 702, 1386, 1657
BstKTI GATC 7 cut(s) 270, 1025, 1063, 1281, 1431, 2043, 2131
BstMAI GTCTC 6 cut(s) 304, 483, 1013, 1218, 1226, 1913
BstMBI GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
BstMCI CGRYCG 1 cut(s) 1849
BstNI CCWGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
BstNSI RCATGY 4 cut(s) 1495, 1538, 1542, 1978
BstPI GGTNACC 1 cut(s) 1738
BstSCI CCNGG 7 cut(s) 660, 875, 1127, 1274, 1388, 1713, 2102
BstSFI CTRYAG 2 cut(s) 1254, 1346
BstUI CGCG 3 cut(s) 702, 1201, 2174
BstV1I GCAGC 7 cut(s) 85, 139, 699, 1240, 1360, 1546, 1639
BstV2I GAAGAC 1 cut(s) 1877
BstX2I RGATCY 3 cut(s) 1022, 1278, 1428
BstXI CCANNNNNNTGG 1 cut(s) 1609
BstYI RGATCY 3 cut(s) 1022, 1278, 1428
BsuI GTATCC 1 cut(s) 594
BsuRI GGCC 4 cut(s) 132, 532, 1160, 1674
BtgI CCRYGG 3 cut(s) 159, 1236, 1588
BtrI CACGTC 2 cut(s) 418, 1771
BtsCI GGATG 7 cut(s) 14, 365, 443, 874, 885, 1016, 2203
BtsI GCAGTG 2 cut(s) 1263, 2149
BtsIMutI CAGTG 6 cut(s) 118, 270, 818, 1263, 1675, 2149
BtuMI TCGCGA 1 cut(s) 1201
BveI ACCTGC 2 cut(s) 1872, 2205
Cac8I GCNNGC 8 cut(s) 84, 178, 774, 1199, 1456, 1497, 1536, 1540
CaiI CAGNNNCTG 2 cut(s) 1330, 2119
CciI TCATGA 1 cut(s) 1411
CfoI GCGC 4 cut(s) 118, 702, 1386, 1657
Cfr13I GGNCC 3 cut(s) 835, 1612, 1673
CseI GACGC 2 cut(s) 530, 1654
Csp6I GTAC 5 cut(s) 127, 445, 550, 1552, 2122
CviQI GTAC 5 cut(s) 127, 445, 550, 1552, 2122
DpnI GATC 7 cut(s) 269, 1024, 1062, 1280, 1430, 2042, 2130
DpnII GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 5 cut(s) 393, 609, 639, 1794, 1846
EarI CTCTTC 5 cut(s) 393, 609, 639, 1794, 1846
EciI GGCGGA 2 cut(s) 571, 723
Ecl136II GAGCTC 2 cut(s) 561, 1843
Eco130I CCWWGG 3 cut(s) 1236, 1588, 1831
Eco24I GRGCYC 3 cut(s) 563, 1780, 1845
Eco31I GGTCTC 2 cut(s) 304, 1226
Eco47I GGWCC 2 cut(s) 835, 1612
Eco53kI GAGCTC 2 cut(s) 561, 1843
Eco57I CTGAAG 7 cut(s) 98, 287, 816, 842, 1490, 1508, 1638
Eco88I CYCGRG 2 cut(s) 1248, 1633
Eco91I GGTNACC 1 cut(s) 1738
EcoICRI GAGCTC 2 cut(s) 561, 1843
EcoNI CCTNNNNNAGG 2 cut(s) 110, 1436
EcoO65I GGTNACC 1 cut(s) 1738
EcoRII CCWGG 7 cut(s) 660, 875, 1127, 1274, 1388, 1713, 2102
EcoT14I CCWWGG 3 cut(s) 1236, 1588, 1831
EcoT22I ATGCAT 1 cut(s) 1540
EcoT38I GRGCYC 3 cut(s) 563, 1780, 1845
ErhI CCWWGG 3 cut(s) 1236, 1588, 1831
FaqI GGGAC 1 cut(s) 1598
FauI CCCGC 2 cut(s) 539, 2179
FauNDI CATATG 2 cut(s) 1465, 1573
FblI GTMKAC 1 cut(s) 323
FokI GGATG 7 cut(s) 21, 352, 450, 881, 892, 1003, 2210
FriOI GRGCYC 3 cut(s) 563, 1780, 1845
FspAI RTGCGCAY 1 cut(s) 1385
FspBI CTAG 3 cut(s) 846, 1112, 1832
FspI TGCGCA 1 cut(s) 1385
GlaI GCGC 4 cut(s) 117, 701, 1385, 1656
GsaI CCCAGC 1 cut(s) 2166
GsuI CTGGAG 3 cut(s) 47, 1736, 1910
HaeIII GGCC 4 cut(s) 132, 532, 1160, 1674
HapII CCGG 3 cut(s) 245, 533, 1161
HgaI GACGC 2 cut(s) 530, 1654
HhaI GCGC 4 cut(s) 118, 702, 1386, 1657
Hin1I GRCGYC 2 cut(s) 522, 1665
Hin6I GCGC 4 cut(s) 116, 700, 1384, 1655
HinP1I GCGC 4 cut(s) 116, 700, 1384, 1655
HincII GTYRAC 3 cut(s) 324, 1038, 1825
HindII GTYRAC 3 cut(s) 324, 1038, 1825
HindIII AAGCTT 2 cut(s) 989, 1756
HpaII CCGG 3 cut(s) 245, 533, 1161
HphI GGTGA 5 cut(s) 471, 595, 733, 1453, 1750
Hpy166II GTNNAC 6 cut(s) 163, 324, 835, 1038, 1825, 1993
Hpy8I GTNNAC 6 cut(s) 163, 324, 835, 1038, 1825, 1993
Hpy99I CGWCG 5 cut(s) 524, 569, 707, 1000, 1559
HpyAV CCTTC 9 cut(s) 29, 590, 617, 718, 791, 817, 1255, 1954, 2177
HpyCH4III ACNGT 4 cut(s) 122, 315, 1182, 2121
HpyCH4IV ACGT 3 cut(s) 417, 1554, 1770
HpySE526I ACGT 3 cut(s) 417, 1554, 1770
Hsp92I GRCGYC 2 cut(s) 522, 1665
HspAI GCGC 4 cut(s) 116, 700, 1384, 1655
Kzo9I GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
LguI GCTCTTC 2 cut(s) 639, 1794
Lsp1109I GCAGC 7 cut(s) 85, 139, 699, 1240, 1360, 1546, 1639
LweI GCATC 4 cut(s) 870, 877, 1025, 2199
MaeI CTAG 3 cut(s) 846, 1112, 1832
MaeII ACGT 3 cut(s) 417, 1554, 1770
MaeIII GTNAC 5 cut(s) 46, 344, 477, 1738, 2134
MalI GATC 7 cut(s) 269, 1024, 1062, 1280, 1430, 2042, 2130
MboI GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
MflI RGATCY 3 cut(s) 1022, 1278, 1428
MhlI GDGCHC 3 cut(s) 563, 1780, 1845
MlyI GAGTC 8 cut(s) 257, 329, 368, 640, 1015, 1313, 1952, 2093
MmeI TCCRAC 4 cut(s) 396, 588, 1383, 1558
Mph1103I ATGCAT 1 cut(s) 1540
MroXI GAANNNNTTC 2 cut(s) 1087, 1099
MseI TTAA 8 cut(s) 575, 927, 1053, 1155, 1206, 1283, 1379, 1545
MslI CAYNNNNRTG 2 cut(s) 351, 1494
MspA1I CMGCKG 4 cut(s) 76, 690, 794, 1652
MspI CCGG 3 cut(s) 245, 533, 1161
MspR9I CCNGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
Mva1269I GAATGC 1 cut(s) 1862
MvaI CCWGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
MvnI CGCG 3 cut(s) 702, 1201, 2174
NcoI CCATGG 2 cut(s) 1236, 1588
NdeI CATATG 2 cut(s) 1465, 1573
NdeII GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
NlaIV GGNNCC 5 cut(s) 258, 1614, 1675, 1893, 2168
NmeAIII GCCGAG 1 cut(s) 1327
NmuCI GTSAC 5 cut(s) 46, 344, 477, 1738, 2134
NruI TCGCGA 1 cut(s) 1201
NsbI TGCGCA 1 cut(s) 1385
NsiI ATGCAT 1 cut(s) 1540
NspI RCATGY 4 cut(s) 1495, 1538, 1542, 1978
OliI CACNNNNGTG 2 cut(s) 351, 1494
PaeI GCATGC 2 cut(s) 1538, 1542
PaeR7I CTCGAG 1 cut(s) 1248
PagI TCATGA 1 cut(s) 1411
PaqCI CACCTGC 2 cut(s) 1872, 2205
PciI ACATGT 1 cut(s) 1974
PciSI GCTCTTC 2 cut(s) 639, 1794
PctI GAATGC 1 cut(s) 1862
PdmI GAANNNNTTC 2 cut(s) 1087, 1099
PfeI GAWTC 6 cut(s) 857, 1088, 1320, 1406, 1786, 2055
PflFI GACNNNGTC 1 cut(s) 1942
PflMI CCANNNNNTGG 1 cut(s) 667
PfoI TCCNGGA 1 cut(s) 875
PleI GAGTC 8 cut(s) 256, 328, 368, 639, 1014, 1312, 1951, 2092
PpsI GAGTC 8 cut(s) 256, 328, 368, 639, 1014, 1312, 1951, 2092
PscI ACATGT 1 cut(s) 1974
Psp124BI GAGCTC 2 cut(s) 563, 1845
Psp6I CCWGG 7 cut(s) 660, 875, 1127, 1274, 1388, 1713, 2102
PspEI GGTNACC 1 cut(s) 1738
PspFI CCCAGC 1 cut(s) 2162
PspGI CCWGG 7 cut(s) 660, 875, 1127, 1274, 1388, 1713, 2102
PspN4I GGNNCC 5 cut(s) 258, 1614, 1675, 1893, 2168
PspPI GGNCC 3 cut(s) 835, 1612, 1673
PspXI VCTCGAGB 1 cut(s) 1248
PstI CTGCAG 2 cut(s) 1258, 1350
PstNI CAGNNNCTG 2 cut(s) 1330, 2119
PsuI RGATCY 3 cut(s) 1022, 1278, 1428
PsyI GACNNNGTC 1 cut(s) 1942
PvuII CAGCTG 3 cut(s) 76, 794, 1652
RruI TCGCGA 1 cut(s) 1201
RsaI GTAC 5 cut(s) 128, 446, 551, 1553, 2123
RsaNI GTAC 5 cut(s) 127, 445, 550, 1552, 2122
RseI CAYNNNNRTG 2 cut(s) 351, 1494
SacI GAGCTC 2 cut(s) 563, 1845
SalI GTCGAC 1 cut(s) 322
SapI GCTCTTC 2 cut(s) 639, 1794
SaqAI TTAA 8 cut(s) 575, 927, 1053, 1155, 1206, 1283, 1379, 1545
Sau3AI GATC 7 cut(s) 267, 1022, 1060, 1278, 1428, 2040, 2128
Sau96I GGNCC 3 cut(s) 835, 1612, 1673
SchI GAGTC 8 cut(s) 257, 329, 368, 640, 1015, 1313, 1952, 2093
ScrFI CCNGG 7 cut(s) 662, 877, 1129, 1276, 1390, 1715, 2104
SduI GDGCHC 3 cut(s) 563, 1780, 1845
SfaNI GCATC 4 cut(s) 870, 877, 1025, 2199
SfcI CTRYAG 2 cut(s) 1254, 1346
Sfr274I CTCGAG 1 cut(s) 1248
SinI GGWCC 2 cut(s) 835, 1612
SlaI CTCGAG 1 cut(s) 1248
SmiMI CAYNNNNRTG 2 cut(s) 351, 1494
SmlI CTYRAG 2 cut(s) 1248, 1750
SmoI CTYRAG 2 cut(s) 1248, 1750
SphI GCATGC 2 cut(s) 1538, 1542
SspMI CTAG 3 cut(s) 846, 1112, 1832
SstI GAGCTC 2 cut(s) 563, 1845
StyD4I CCNGG 7 cut(s) 660, 875, 1127, 1274, 1388, 1713, 2102
StyI CCWWGG 3 cut(s) 1236, 1588, 1831
TaaI ACNGT 4 cut(s) 122, 315, 1182, 2121
TaiI ACGT 3 cut(s) 420, 1557, 1773
TaqI TCGA 5 cut(s) 323, 519, 1249, 1845, 2082
TaqII GACCGA 1 cut(s) 1863
TatI WGTACW 1 cut(s) 2121
TauI GCSGC 5 cut(s) 158, 244, 257, 743, 2190
TfiI GAWTC 6 cut(s) 857, 1088, 1320, 1406, 1786, 2055
Tru1I TTAA 8 cut(s) 575, 927, 1053, 1155, 1206, 1283, 1379, 1545
Tru9I TTAA 8 cut(s) 575, 927, 1053, 1155, 1206, 1283, 1379, 1545
TscAI CASTG 6 cut(s) 125, 277, 825, 1263, 1675, 2149
TseFI GTSAC 5 cut(s) 46, 344, 477, 1738, 2134
TseI GCWGC 8 cut(s) 73, 152, 687, 1253, 1348, 1559, 1652, 2211
Tsp45I GTSAC 5 cut(s) 46, 344, 477, 1738, 2134
TspDTI ATGAA 5 cut(s) 345, 969, 1161, 1353, 1428
TspGWI ACGGA 3 cut(s) 1061, 1955, 2067
TspRI CASTG 6 cut(s) 125, 277, 825, 1263, 1675, 2149
Tth111I GACNNNGTC 1 cut(s) 1942
Van91I CCANNNNNTGG 1 cut(s) 667
VpaK11BI GGWCC 2 cut(s) 835, 1612
XagI CCTNNNNNAGG 2 cut(s) 110, 1436
XapI RAATTY 1 cut(s) 1049
XbaI TCTAGA 1 cut(s) 1111
XceI RCATGY 4 cut(s) 1495, 1538, 1542, 1978
XhoI CTCGAG 1 cut(s) 1248
XmaJI CCTAGG 1 cut(s) 1831
XmiI GTMKAC 1 cut(s) 323
XmnI GAANNNNTTC 2 cut(s) 1087, 1099
XspI CTAG 3 cut(s) 846, 1112, 1832
Zsp2I ATGCAT 1 cut(s) 1540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.