pycom05g11840

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014658
Physical Location & Seq
Forward (+)
15400876 .. 15402387
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g11840.1

Sequence Viewer

Length: 1329 bp
ATGAAGCTAATAATTCTTCACTTATCCTATTTCAATGCAGAGAACTCAACAAAGCTAATTCAAGCTATAACATGGCATAACTCGACTTCATCGTCTAAGCTTTCGTCGTGCAAGAGTCTCGTGGCATCCAGATCTAAGGGTTCGTCAACATGGACGGAATTTAACAATGATCTTTTCAATGATTATGGAGGAATGGATTCAGGAAGTCATTCACTTACTCTAGAAAGGCTATATGCCTGGGAAAAGAAGCTCTATGAAGAAGTTAAGGCTGGAGATATGACAAGGAAACTGTATGAGAAAAAATGCTCACGACTTAAACACCACGACATAGGAGACAACGAGACTATGGACAAAACTCGAGCTGCAGTAAAGGATTTATATGCCAGGATCTTAATTGCAATTCGGAGTGCTGAGTCAATCTCCAAGAGAATCCAGATACTGAGAGATGAAGAACTGCAGCCTCAAATTGTGGAGCTGTTGAAAGGCTTAATGCACACCTGGAAAATTATGTTGGAATCTCATGAAACCCAATACAAGATCCTTTCCGAGGTGAAATCTTTTGCTGGCTCTGCATATGGAAAGTTCAGCAACATCTCTCACATGCGTGCAACGCTTCAGCTCCTCGCTGAGCTTCAGAATTGGCATGCATGCTTTAAAGAGTACGTCGCTGCACAAAAAGCATATGTTGAAACTCTCCATGGTTGGCTAACCAAGTTTGTGATCCCTGAAGAAAACTTCTACTCGGGGAGCAGGAGTTCAGCTGTGCCATTTGGCGTCAATGGCCCCCCGTTGCTCATTATCTGCCATGATTGGTTATCTTCCATGGAGAAATTACCAGATACTTTGGTGACCATTGCCTTGAGAAGCTTTACAAAGGACGTGAGGGCTCTGTTGAATCAGCAAGGGGAAGAGCAGAAACAAAAAAGGAAAGTTGACAGCCTAGCCAAAGAGCTCGACCGAAGAATTCGTGCATTCCAGAAGACAGAGAGCAGGTTTCTGGAGCCAAAGCTCACAGAACAGAAATGTGAGACAGATATGGAACATAAGACCGAGTCCTTGACAGAGAAGAAGGAGCAGATAGATATGTTCAGAAGAAAGGTGGACATGGAGAGGGAAAAACACCAAAATTACGTGGAGGAAACTCAAAGGATCACATTGAATGGAATCCAGACTGGGTTTTGTTCAGTTTTCGAGTCCTTAGCGGAGTTTTCCAGGGCTTCACAGAAACTGTACAGCGATCTTGTGACTTGCAGTGAAAATGCAGATAAAGCTGGGAACCCCTCATATATAGAAGGCAGCACCAAGGATGACGAAAACAGCAGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

443

Amino Acids

50.82

Weight (kDa)

8.02

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 14 - 295 3.9e-93 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014658)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15580
malus_domestica MD05G1124400.v1.1 MD10G1127200.v1.1
prunus_persica Prupe.8G168100_v2.0.a1
pyrus_communis pycom05g11840
rosa_chinensis RchiOBHm_Chr6g0278761
rosa_laevigata RLG00000013195
rosa_multiflora Rmu_sc0004380.1_g000001 Rmu_sc0027143.1_g000002
rosa_roxburghii Rroxscaffold_7G00189650 Rroxscaffold_7G00189920
rosa_rugosa Rorug06G0118700.1
rosa_samantha Rh6AG227500 Rh6BG233100 Rh6CG235300 Rh6DG226800
rosa_wichuraiana Rw6G019960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1314
AasI GACNNNNNNGTC 1 cut(s) 91
Acc36I ACCTGC 2 cut(s) 981, 1314
AciI CCGC 1 cut(s) 1202
AclWI GGATC 4 cut(s) 395, 532, 715, 1157
AcsI RAATTY 2 cut(s) 158, 963
AcuI CTGAAG 3 cut(s) 599, 617, 747
AcyI GRCGYC 1 cut(s) 774
AfaI GTAC 2 cut(s) 662, 1232
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 7 cut(s) 34, 62, 178, 481, 689, 895, 1159
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 4 cut(s) 236, 383, 497, 1211
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AleI CACNNNNGTG 1 cut(s) 603
AloI GAACNNNNNNTCC 2 cut(s) 739, 771
Alw21I GWGCWC 1 cut(s) 954
Alw26I GTCTC 4 cut(s) 122, 327, 335, 1022
AlwI GGATC 4 cut(s) 395, 532, 715, 1157
AlwNI CAGNNNCTG 2 cut(s) 439, 1228
Ama87I CYCGRG 2 cut(s) 357, 742
AoxI GGCC 1 cut(s) 781
ApeKI GCWGC 5 cut(s) 362, 457, 668, 1296, 1320
ApoI RAATTY 2 cut(s) 158, 963
Asp700I GAANNNNTTC 2 cut(s) 196, 208
AspS9I GGNCC 1 cut(s) 782
AsuHPI GGTGA 2 cut(s) 562, 859
AvaI CYCGRG 2 cut(s) 357, 742
BaeI ACNNNNGTAYC 2 cut(s) 831, 864
BanII GRGCYC 2 cut(s) 889, 954
BauI CACGAG 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 986
Bbv12I GWGCWC 1 cut(s) 954
BbvI GCAGC 4 cut(s) 349, 469, 655, 1308
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 4 cut(s) 238, 385, 499, 1213
BcoDI GTCTC 4 cut(s) 122, 327, 335, 1022
BfaI CTAG 2 cut(s) 221, 941
BfmI CTRYAG 2 cut(s) 363, 455
BfuAI ACCTGC 2 cut(s) 981, 1314
BglII AGATCT 1 cut(s) 131
BisI GCNGC 5 cut(s) 363, 458, 669, 1297, 1321
BlpI GCTNAGC 1 cut(s) 627
BlsI GCNGC 5 cut(s) 364, 459, 670, 1298, 1322
Bme1390I CCNGG 4 cut(s) 238, 385, 499, 1213
BmeT110I CYCGRG 2 cut(s) 357, 742
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 3 cut(s) 784, 1002, 1277
BmrFI CCNGG 4 cut(s) 238, 385, 499, 1213
BmrI ACTGGG 1 cut(s) 1182
BmsI GCATC 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 1182
BpiI GAAGAC 1 cut(s) 986
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpmI CTGGAG 2 cut(s) 291, 1019
Bpu10I CCTNAGC 1 cut(s) 1198
Bpu1102I GCTNAGC 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 880
BsaAI YACGTR 1 cut(s) 1132
BsaHI GRCGYC 1 cut(s) 774
BsaJI CCNNGG 6 cut(s) 237, 546, 697, 822, 1212, 1302
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 1177
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 4 cut(s) 238, 385, 499, 1213
BseDI CCNNGG 6 cut(s) 237, 546, 697, 822, 1212, 1302
BseGI GGATG 2 cut(s) 125, 1312
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 3 cut(s) 402, 431, 618
BseNI ACTGG 1 cut(s) 1177
BseRI GAGGAG 1 cut(s) 611
BseXI GCAGC 4 cut(s) 349, 469, 655, 1308
BseYI CCCAGC 1 cut(s) 1271
BsgI GTGCAG 1 cut(s) 654
Bsh1285I CGRYCG 1 cut(s) 958
BshFI GGCC 1 cut(s) 783
BsiEI CGRYCG 1 cut(s) 958
BsiHKAI GWGCWC 1 cut(s) 954
BsiHKCI CYCGRG 2 cut(s) 357, 742
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 4 cut(s) 122, 327, 335, 1022
BsmI GAATGC 1 cut(s) 971
BsnI GGCC 1 cut(s) 783
BsoBI CYCGRG 2 cut(s) 357, 742
Bsp1286I GDGCHC 2 cut(s) 889, 954
Bsp1407I TGTACA 1 cut(s) 1230
Bsp143I GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
Bsp1720I GCTNAGC 1 cut(s) 627
Bsp19I CCATGG 2 cut(s) 697, 822
BspACI CCGC 1 cut(s) 1202
BspANI GGCC 1 cut(s) 783
BspCNI CTCAG 3 cut(s) 403, 432, 619
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 3 cut(s) 784, 1002, 1277
BspMAI CTGCAG 2 cut(s) 367, 459
BspMI ACCTGC 2 cut(s) 981, 1314
BspPI GGATC 4 cut(s) 395, 532, 715, 1157
BspQI GCTCTTC 1 cut(s) 903
BsrDI GCAATG 1 cut(s) 852
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 1 cut(s) 1177
BssECI CCNNGG 6 cut(s) 237, 546, 697, 822, 1212, 1302
BssMI GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
BssNI GRCGYC 1 cut(s) 774
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 3 cut(s) 697, 822, 1302
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 4 cut(s) 238, 385, 499, 1213
Bst4CI ACNGT 2 cut(s) 291, 1230
Bst6I CTCTTC 1 cut(s) 903
BstACI GRCGYC 1 cut(s) 774
BstAUI TGTACA 1 cut(s) 1230
BstBAI YACGTR 1 cut(s) 1132
BstC8I GCNNGC 4 cut(s) 565, 606, 645, 649
BstDEI CTNAG 6 cut(s) 96, 135, 411, 440, 627, 1198
BstDSI CCRYGG 2 cut(s) 697, 822
BstEII GGTNACC 1 cut(s) 847
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 2 cut(s) 125, 1312
BstKTI GATC 7 cut(s) 134, 172, 390, 540, 723, 1152, 1240
BstMAI GTCTC 4 cut(s) 122, 327, 335, 1022
BstMBI GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
BstMCI CGRYCG 1 cut(s) 958
BstMWI GCNNNNNNNGC 5 cut(s) 569, 610, 677, 780, 1268
BstNI CCWGG 4 cut(s) 238, 385, 499, 1213
BstNSI RCATGY 3 cut(s) 604, 647, 651
BstPI GGTNACC 1 cut(s) 847
BstSCI CCNGG 4 cut(s) 236, 383, 497, 1211
BstSFI CTRYAG 2 cut(s) 363, 455
BstV1I GCAGC 4 cut(s) 349, 469, 655, 1308
BstV2I GAAGAC 1 cut(s) 986
BstX2I RGATCY 3 cut(s) 131, 387, 537
BstYI RGATCY 3 cut(s) 131, 387, 537
BsuRI GGCC 1 cut(s) 783
BtgI CCRYGG 2 cut(s) 697, 822
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 2 cut(s) 125, 1312
BtsI GCAGTG 1 cut(s) 1258
BtsIMutI CAGTG 1 cut(s) 1258
BveI ACCTGC 2 cut(s) 981, 1314
Cac8I GCNNGC 4 cut(s) 565, 606, 645, 649
CaiI CAGNNNCTG 2 cut(s) 439, 1228
CciI TCATGA 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 782
CseI GACGC 1 cut(s) 763
Csp6I GTAC 2 cut(s) 661, 1231
CviQI GTAC 2 cut(s) 661, 1231
DdeI CTNAG 6 cut(s) 96, 135, 411, 440, 627, 1198
DpnI GATC 7 cut(s) 133, 171, 389, 539, 722, 1151, 1239
DpnII GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
DraI TTTAAA 1 cut(s) 655
DrdI GACNNNNNNGTC 1 cut(s) 91
DseDI GACNNNNNNGTC 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
Ecl136II GAGCTC 1 cut(s) 952
Eco130I CCWWGG 3 cut(s) 697, 822, 1302
Eco24I GRGCYC 2 cut(s) 889, 954
Eco53kI GAGCTC 1 cut(s) 952
Eco57I CTGAAG 3 cut(s) 599, 617, 747
Eco88I CYCGRG 2 cut(s) 357, 742
Eco91I GGTNACC 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 952
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoO65I GGTNACC 1 cut(s) 847
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 4 cut(s) 236, 383, 497, 1211
EcoT14I CCWWGG 3 cut(s) 697, 822, 1302
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 2 cut(s) 889, 954
ErhI CCWWGG 3 cut(s) 697, 822, 1302
FauNDI CATATG 2 cut(s) 574, 682
Fnu4HI GCNGC 5 cut(s) 363, 458, 669, 1297, 1321
FokI GGATG 2 cut(s) 112, 1319
FriOI GRGCYC 2 cut(s) 889, 954
Fsp4HI GCNGC 5 cut(s) 363, 458, 669, 1297, 1321
FspBI CTAG 2 cut(s) 221, 941
GluI GCNGC 5 cut(s) 363, 458, 669, 1297, 1321
GsaI CCCAGC 1 cut(s) 1275
GsuI CTGGAG 2 cut(s) 291, 1019
HaeIII GGCC 1 cut(s) 783
HgaI GACGC 1 cut(s) 763
Hin1I GRCGYC 1 cut(s) 774
HincII GTYRAC 2 cut(s) 147, 934
HindII GTYRAC 2 cut(s) 147, 934
HindIII AAGCTT 2 cut(s) 98, 865
HinfI GANTC 9 cut(s) 115, 197, 413, 429, 515, 895, 1052, 1164, 1193
HphI GGTGA 2 cut(s) 562, 859
Hpy166II GTNNAC 3 cut(s) 147, 934, 1102
Hpy188I TCNGA 4 cut(s) 405, 547, 636, 1091
Hpy188III TCNNGA 9 cut(s) 129, 201, 221, 309, 433, 521, 976, 998, 1168
Hpy8I GTNNAC 3 cut(s) 147, 934, 1102
Hpy99I CGWCG 2 cut(s) 109, 668
HpyAV CCTTC 2 cut(s) 1063, 1286
HpyCH4III ACNGT 2 cut(s) 291, 1230
HpyCH4IV ACGT 3 cut(s) 663, 879, 1131
HpyF10VI GCNNNNNNNGC 5 cut(s) 569, 610, 677, 780, 1268
HpyF3I CTNAG 6 cut(s) 96, 135, 411, 440, 627, 1198
HpySE526I ACGT 3 cut(s) 663, 879, 1131
Hsp92I GRCGYC 1 cut(s) 774
Kzo9I GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
LguI GCTCTTC 1 cut(s) 903
LmnI GCTCC 5 cut(s) 472, 624, 747, 1000, 1072
Lsp1109I GCAGC 4 cut(s) 349, 469, 655, 1308
LweI GCATC 1 cut(s) 134
MaeI CTAG 2 cut(s) 221, 941
MaeII ACGT 3 cut(s) 663, 879, 1131
MaeIII GTNAC 2 cut(s) 847, 1243
MalI GATC 7 cut(s) 133, 171, 389, 539, 722, 1151, 1239
MboI GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
MflI RGATCY 3 cut(s) 131, 387, 537
MhlI GDGCHC 2 cut(s) 889, 954
MlyI GAGTC 4 cut(s) 124, 422, 1061, 1202
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 8 cut(s) 182, 471, 541, 632, 876, 1104, 1129, 1291
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 2 cut(s) 196, 208
MseI TTAA 6 cut(s) 162, 264, 315, 392, 488, 654
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 761
MspR9I CCNGG 4 cut(s) 238, 385, 499, 1213
Mva1269I GAATGC 1 cut(s) 971
MvaI CCWGG 4 cut(s) 238, 385, 499, 1213
MwoI GCNNNNNNNGC 5 cut(s) 569, 610, 677, 780, 1268
NcoI CCATGG 2 cut(s) 697, 822
NdeI CATATG 2 cut(s) 574, 682
NdeII GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
NlaIV GGNNCC 3 cut(s) 784, 1002, 1277
NmuCI GTSAC 2 cut(s) 847, 1243
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 3 cut(s) 604, 647, 651
OliI CACNNNNGTG 1 cut(s) 603
PaeI GCATGC 2 cut(s) 647, 651
PaeR7I CTCGAG 1 cut(s) 357
PagI TCATGA 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 1314
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 1 cut(s) 971
PdmI GAANNNNTTC 2 cut(s) 196, 208
PfeI GAWTC 5 cut(s) 197, 429, 515, 895, 1164
PflFI GACNNNGTC 1 cut(s) 1051
PkrI GCNGC 5 cut(s) 364, 459, 670, 1298, 1322
PleI GAGTC 4 cut(s) 123, 421, 1060, 1201
PpsI GAGTC 4 cut(s) 123, 421, 1060, 1201
Ppu21I YACGTR 1 cut(s) 1132
Psp124BI GAGCTC 1 cut(s) 954
Psp6I CCWGG 4 cut(s) 236, 383, 497, 1211
PspEI GGTNACC 1 cut(s) 847
PspFI CCCAGC 1 cut(s) 1271
PspGI CCWGG 4 cut(s) 236, 383, 497, 1211
PspN4I GGNNCC 3 cut(s) 784, 1002, 1277
PspPI GGNCC 1 cut(s) 782
PspXI VCTCGAGB 1 cut(s) 357
PstI CTGCAG 2 cut(s) 367, 459
PstNI CAGNNNCTG 2 cut(s) 439, 1228
PsuI RGATCY 3 cut(s) 131, 387, 537
PsyI GACNNNGTC 1 cut(s) 1051
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 662, 1232
RsaNI GTAC 2 cut(s) 661, 1231
RseI CAYNNNNRTG 1 cut(s) 603
SacI GAGCTC 1 cut(s) 954
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 6 cut(s) 162, 264, 315, 392, 488, 654
SatI GCNGC 5 cut(s) 363, 458, 669, 1297, 1321
Sau3AI GATC 7 cut(s) 131, 169, 387, 537, 720, 1149, 1237
Sau96I GGNCC 1 cut(s) 782
SchI GAGTC 4 cut(s) 124, 422, 1061, 1202
ScrFI CCNGG 4 cut(s) 238, 385, 499, 1213
SduI GDGCHC 2 cut(s) 889, 954
SfaNI GCATC 1 cut(s) 134
SfcI CTRYAG 2 cut(s) 363, 455
Sfr274I CTCGAG 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 357, 859
SmoI CTYRAG 2 cut(s) 357, 859
SphI GCATGC 2 cut(s) 647, 651
SsiI CCGC 1 cut(s) 1202
SspMI CTAG 2 cut(s) 221, 941
SstI GAGCTC 1 cut(s) 954
StyD4I CCNGG 4 cut(s) 236, 383, 497, 1211
StyI CCWWGG 3 cut(s) 697, 822, 1302
TaaI ACNGT 2 cut(s) 291, 1230
TaiI ACGT 3 cut(s) 666, 882, 1134
TaqI TCGA 4 cut(s) 83, 358, 954, 1191
TaqII GACCGA 2 cut(s) 972, 1064
TatI WGTACW 1 cut(s) 1230
TfiI GAWTC 5 cut(s) 197, 429, 515, 895, 1164
Tru1I TTAA 6 cut(s) 162, 264, 315, 392, 488, 654
Tru9I TTAA 6 cut(s) 162, 264, 315, 392, 488, 654
TscAI CASTG 1 cut(s) 1258
TseFI GTSAC 2 cut(s) 847, 1243
TseI GCWGC 5 cut(s) 362, 457, 668, 1296, 1320
Tsp45I GTSAC 2 cut(s) 847, 1243
TspDTI ATGAA 5 cut(s) 17, 78, 270, 462, 537
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 1 cut(s) 1258
Tth111I GACNNNGTC 1 cut(s) 1051
XagI CCTNNNNNAGG 1 cut(s) 545
XapI RAATTY 2 cut(s) 158, 963
XbaI TCTAGA 1 cut(s) 220
XceI RCATGY 3 cut(s) 604, 647, 651
XhoI CTCGAG 1 cut(s) 357
XmnI GAANNNNTTC 2 cut(s) 196, 208
XspI CTAG 2 cut(s) 221, 941
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.