MD10G1127200.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014658
Physical Location & Seq
Forward (+)
20744137 .. 20748033
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1127200.v1.1.491

Sequence Viewer

Length: 2202 bp
ATGGGGGGATGTGTTGCCTCCAAGCTGGGGGAAGAAGGAGGAGTTGTGACAATTTGTAGGGAGAGAAAGCGGCAGCTGAAGCTGGCGGTGGAGAAAAGGTACGCTCTTGCGGAGGCGCATTGTAAGTACTGCCATGCTTTGTTTGCGGTGGCTGCCGCCGTGGACTTCTTTGTTGCAGGCTACTCTTCCCCTCCTAAACCTTACCTCATCACATTTTCACCGCACTCTACTCCGGCGGCAGAGGCGGAGGTGGCGGCTCCGCTTCCGATCACTGAAAATGTAATAACCAACCCAATGTTCCTTCAGCATAGGCCGTCTGAGTCGACCCATGAGTCCATTGCTTGTCACTCACGTGGTTCTTCAACGTCCTCAGACTCCTCTGAGGAGGAGAGAGAAGAAGAGGTGCAAAAAACAGAAGAGAGAGAAGAACATGGATGTGGGTACTTTTATATGGAAATGCCACCAGCAATGCTGTCACCACAGAGAGATTTTGGGTGGGATTTCTTCAACCCGTTCGATGCCGTGAGGCCGGAGGTTATAAGCGGGTACAGGCGGAGCTCCGACGAGGATCTGAGGGTGGTGAGGGAGGAGGAAGGGATACCCGAACTAGAAGAGGAAGAAGAGGAGAGTCAGGTAGAAAACAAGGCAGTGGTGGTGGAAGATAACGGCGGAATAGAGCAGCATGACGGCGGAGCTGAGGTGGTGAAGGTGGTTGATGAGGGGGATAATGTGAGGGAGGGGGAGCAAAAGGGGCTTGCTGTGATTGATACACCAGCTGAAGGGAGGGAGCTGTTGGAAGCATTGAAGGACATTGTGGACCTTTTTTCTAGGGCTTCTGATTCTGCAAAGGACGTGTCCAGGATGCTTGATGCTAATAAACTGCAAATGCAGTCTGGTTTGGAGGAAATTAAAGAGAACTCAACAAAGCTAATTGAAGCTATAACATGGCATAACTCGACTTCATCGTCTAAGCTTTCGTCATGTAAGAGTCTTGTGGCATCGAGATCTAAGGGTTCGTCAACATGGACGGAATTTAAGAATGATCTTTTCGATGATTATGGTGGAATGGATTCAGGAAGTCATTCACTTACTCTGGAAAGGCTATATGCCTGGGAAAAGAAGCTCTATGACCAAGTTAAGGCCGGAGATATGACACGGAAACTTTATGAGAAAAAATGCTCGCGACTTAAACACCACGACATAGGAGACAACGAGACCATGGACAAAACTCAAGCTGCAGTAAAGGATTTATACGCCAGAATCTTAATTGCAATTCGGAGTGCCGAGTCAATTTCCAAGAGAATCCAGATATTGAGAGATGATGAACTGCAGCCTCAAATTGTTGAGTTGTTGAAAGGCTTAATGCGCACCTGGAAAATTATGTTGGAATCTCATGAAACCCAACACAAGATCCTTTTCGAGGTGAAATCTTTTGCTGGCTCTGCGTATGGAAAGTTCAGCAACAACTCTCACATGCGTGCTACGCTTCAGCTTCTTGTCGAGCTTCAGAATTGGCATGCGTGCTTTAGGGAGTACGTCGCTGCACAAAAAGCATATGTTGAAACTCTCCACGGTTGGCTAATTAAGTTTGTGGTCCCTGAGGAGGAATTATACTCCAGGAGCAGGAGTTCAGCTGCTCCGTATGGGGTTAATGGCCCCCCGTTGCTCTTGATCTGCCATGATTGGTTATCTTCAATGGAGAAATTACCGGATACTTTGGTGACCATTGCCTTGAGAAGCTTTACAAAGGACGTGAGGGCTCTGTTGAATCAGCAAGGGGAAGAGCAGAAACAAAAACGGAAAGTTGACAGCCTAGCCAAAGAGCTCGACCGAAGAATTCGTGCATTCCAGAAGACAGAGAGCAGGTTTCTCGAGCCAAAGCTCGTGGAACAGAAATGCGAGACAGATATTGAACATAAGACCGAGTCCTTGACAGAAAGAAAGGAGCAGATAGACATGTTCAGAAGAAAGGTGGACATGGAGAGGGAAAAACACCAAAACTACGTGCAGGAAACTCAAAGGGTCACATTAAACGTAATCCAGACTGGGTTTTGTTCAGTTTTCGAGTCCTTAGCGGAGTTCTCCAGGGCTTCACAGAAACTGTACGGCGATCTCGTGACCTGCAGCGAAAATGCAGATAAAGCTGGGAACCCGGCGTATATAGAAGGCGGCACCAAGGATGACGAAAATGGCAGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

734

Amino Acids

82.98

Weight (kDa)

5.34

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 3 - 59 4.6e-17 Protein of unknown function (DUF630)
DUF632 PF04782 264 - 586 1.2e-102 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014658)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15580
malus_domestica MD05G1124400.v1.1 MD10G1127200.v1.1
prunus_persica Prupe.8G168100_v2.0.a1
pyrus_communis pycom05g11840
rosa_chinensis RchiOBHm_Chr6g0278761
rosa_laevigata RLG00000013195
rosa_multiflora Rmu_sc0004380.1_g000001 Rmu_sc0027143.1_g000002
rosa_roxburghii Rroxscaffold_7G00189650 Rroxscaffold_7G00189920
rosa_rugosa Rorug06G0118700.1
rosa_samantha Rh6AG227500 Rh6BG233100 Rh6CG235300 Rh6DG226800
rosa_wichuraiana Rw6G019960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AarI CACCTGC 1 cut(s) 2187
AasI GACNNNNNNGTC 2 cut(s) 331, 964
Acc16I TGCGCA 1 cut(s) 1367
Acc36I ACCTGC 3 cut(s) 1854, 2129, 2187
AccB1I GGYRCC 1 cut(s) 2171
AccI GTMKAC 1 cut(s) 323
AccII CGCG 1 cut(s) 1183
AclWI GGATC 2 cut(s) 576, 1405
AcsI RAATTY 2 cut(s) 1031, 1836
AcuI CTGAAG 5 cut(s) 98, 287, 798, 1472, 1490
AcvI CACGTG 1 cut(s) 353
AfaI GTAC 6 cut(s) 101, 128, 443, 548, 1535, 2105
AfiI CCNNNNNNNGG 6 cut(s) 27, 779, 1138, 1420, 1603, 1623
AflIII ACRYGT 2 cut(s) 852, 1956
AgsI TTSAA 9 cut(s) 363, 508, 805, 935, 1354, 1562, 1695, 1768, 1913
AjiI CACGTC 2 cut(s) 853, 1753
AjnI CCWGG 5 cut(s) 857, 1109, 1370, 1616, 2084
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 1367, 1399
AleI CACNNNNGTG 2 cut(s) 351, 1476
AloI GAACNNNNNNTCC 2 cut(s) 1612, 1644
Alw21I GWGCWC 2 cut(s) 560, 1827
Alw26I GTCTC 3 cut(s) 1200, 1208, 1895
AlwI GGATC 2 cut(s) 576, 1405
AlwNI CAGNNNCTG 1 cut(s) 2101
Ama87I CYCGRG 1 cut(s) 1871
AoxI GGCC 4 cut(s) 311, 527, 1140, 1654
ApeKI GCWGC 9 cut(s) 73, 152, 679, 1235, 1330, 1541, 1634, 2124, 2193
ApoI RAATTY 2 cut(s) 1031, 1836
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
Asp700I GAANNNNTTC 2 cut(s) 1069, 1081
AspLEI GCGC 2 cut(s) 118, 1368
AspS9I GGNCC 3 cut(s) 817, 1594, 1655
AsuC2I CCSGG 1 cut(s) 2153
AsuHPI GGTGA 6 cut(s) 210, 468, 592, 715, 1435, 1732
AvaI CYCGRG 1 cut(s) 1871
AvaII GGWCC 2 cut(s) 817, 1594
AxyI CCTNAGG 1 cut(s) 1599
BaeI ACNNNNGTAYC 2 cut(s) 1704, 1737
BanI GGYRCC 1 cut(s) 2171
BanII GRGCYC 3 cut(s) 560, 1762, 1827
BauI CACGAG 2 cut(s) 1883, 2114
BbrPI CACGTG 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 1859
Bbv12I GWGCWC 2 cut(s) 560, 1827
BbvCI CCTCAGC 1 cut(s) 696
BbvI GCAGC 8 cut(s) 85, 139, 691, 1222, 1342, 1528, 1621, 2136
BceAI ACGGC 6 cut(s) 143, 298, 506, 682, 703, 2122
BcgI CGANNNNNNTGC 2 cut(s) 1852, 1886
BciT130I CCWGG 5 cut(s) 859, 1111, 1372, 1618, 2086
BciVI GTATCC 2 cut(s) 591, 1705
BcnI CCSGG 1 cut(s) 2153
BcoDI GTCTC 3 cut(s) 1200, 1208, 1895
BfaI CTAG 3 cut(s) 608, 828, 1814
BfmI CTRYAG 3 cut(s) 1236, 1328, 2122
BfuAI ACCTGC 3 cut(s) 1854, 2129, 2187
BfuI GTATCC 2 cut(s) 591, 1705
BglII AGATCT 1 cut(s) 1004
BmcAI AGTACT 1 cut(s) 128
Bme1390I CCNGG 6 cut(s) 859, 1111, 1372, 1618, 2086, 2153
Bme18I GGWCC 2 cut(s) 817, 1594
BmeT110I CYCGRG 1 cut(s) 1871
BmgBI CACGTC 2 cut(s) 853, 1753
BmgT120I GGNCC 3 cut(s) 817, 1594, 1655
BmiI GGNNCC 5 cut(s) 258, 1596, 1657, 2150, 2173
BmrFI CCNGG 6 cut(s) 859, 1111, 1372, 1618, 2086, 2153
BmrI ACTGGG 1 cut(s) 2055
BmsI GCATC 4 cut(s) 508, 852, 859, 1007
BmuI ACTGGG 1 cut(s) 2055
BpiI GAAGAC 1 cut(s) 1859
BpmI CTGGAG 2 cut(s) 1600, 2068
Bpu10I CCTNAGC 2 cut(s) 696, 2071
BpuEI CTTGAG 2 cut(s) 1215, 1753
BpuMI CCSGG 1 cut(s) 2153
BsaAI YACGTR 2 cut(s) 353, 2005
BsaI GGTCTC 1 cut(s) 1208
BsaJI CCNNGG 6 cut(s) 159, 1110, 1218, 1570, 2085, 2175
BsaWI WCCGGW 1 cut(s) 1708
BsaXI ACNNNNNCTCC 8 cut(s) 30, 60, 83, 113, 684, 714, 1612, 1642
Bsc4I CCNNNNNNNGG 6 cut(s) 27, 779, 1138, 1420, 1603, 1623
Bse1I ACTGG 1 cut(s) 2050
Bse21I CCTNAGG 1 cut(s) 1599
Bse3DI GCAATG 3 cut(s) 336, 474, 1725
BseBI CCWGG 5 cut(s) 859, 1111, 1372, 1618, 2086
BseDI CCNNGG 6 cut(s) 159, 1110, 1218, 1570, 2085, 2175
BseGI GGATG 4 cut(s) 14, 440, 867, 2185
BseLI CCNNNNNNNGG 6 cut(s) 27, 779, 1138, 1420, 1603, 1623
BseMI GCAATG 3 cut(s) 336, 474, 1725
BseMII CTCAG 6 cut(s) 309, 372, 384, 563, 687, 1590
BseNI ACTGG 1 cut(s) 2050
BseRI GAGGAG 7 cut(s) 54, 367, 398, 401, 602, 638, 1616
BseXI GCAGC 8 cut(s) 85, 139, 691, 1222, 1342, 1528, 1621, 2136
BseYI CCCAGC 2 cut(s) 25, 2144
BsgI GTGCAG 2 cut(s) 1527, 2027
Bsh1236I CGCG 1 cut(s) 1183
Bsh1285I CGRYCG 1 cut(s) 1831
BshFI GGCC 4 cut(s) 313, 529, 1142, 1656
BshNI GGYRCC 1 cut(s) 2171
BsiEI CGRYCG 1 cut(s) 1831
BsiHKAI GWGCWC 2 cut(s) 560, 1827
BsiHKCI CYCGRG 1 cut(s) 1871
BsiSI CCGG 5 cut(s) 233, 530, 1143, 1709, 2153
BslFI GGGAC 1 cut(s) 1580
BslI CCNNNNNNNGG 6 cut(s) 27, 779, 1138, 1420, 1603, 1623
BsmAI GTCTC 3 cut(s) 1200, 1208, 1895
BsmFI GGGAC 1 cut(s) 1580
BsmI GAATGC 1 cut(s) 1844
BsnI GGCC 4 cut(s) 313, 529, 1142, 1656
Bso31I GGTCTC 1 cut(s) 1208
BsoBI CYCGRG 1 cut(s) 1871
Bsp1286I GDGCHC 3 cut(s) 560, 1762, 1827
Bsp143I GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
Bsp19I CCATGG 1 cut(s) 1218
Bsp68I TCGCGA 1 cut(s) 1183
BspANI GGCC 4 cut(s) 313, 529, 1142, 1656
BspCNI CTCAG 6 cut(s) 310, 373, 383, 564, 688, 1591
BspFNI CGCG 1 cut(s) 1183
BspHI TCATGA 1 cut(s) 1393
BspLI GGNNCC 5 cut(s) 258, 1596, 1657, 2150, 2173
BspMAI CTGCAG 3 cut(s) 1240, 1332, 2126
BspMI ACCTGC 3 cut(s) 1854, 2129, 2187
BspPI GGATC 2 cut(s) 576, 1405
BspQI GCTCTTC 1 cut(s) 1776
BspT107I GGYRCC 1 cut(s) 2171
BspTNI GGTCTC 1 cut(s) 1208
BsrDI GCAATG 3 cut(s) 336, 474, 1725
BsrI ACTGG 1 cut(s) 2050
BssECI CCNNGG 6 cut(s) 159, 1110, 1218, 1570, 2085, 2175
BssMI GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
BssSI CACGAG 2 cut(s) 1883, 2114
BssT1I CCWWGG 2 cut(s) 1218, 2175
Bst2BI CACGAG 2 cut(s) 1883, 2114
Bst2UI CCWGG 5 cut(s) 859, 1111, 1372, 1618, 2086
Bst4CI ACNGT 2 cut(s) 1574, 2103
Bst6I CTCTTC 6 cut(s) 190, 393, 411, 606, 615, 1776
BstBAI YACGTR 2 cut(s) 353, 2005
BstC8I GCNNGC 8 cut(s) 84, 178, 756, 1181, 1438, 1479, 1518, 1522
BstDEI CTNAG 9 cut(s) 318, 370, 381, 572, 696, 969, 1008, 1599, 2071
BstDSI CCRYGG 3 cut(s) 159, 1218, 1570
BstEII GGTNACC 1 cut(s) 1720
BstENI CCTNNNNNAGG 1 cut(s) 1418
BstF5I GGATG 4 cut(s) 14, 440, 867, 2185
BstFNI CGCG 1 cut(s) 1183
BstHHI GCGC 2 cut(s) 118, 1368
BstKTI GATC 7 cut(s) 270, 571, 1007, 1045, 1413, 1674, 2113
BstMAI GTCTC 3 cut(s) 1200, 1208, 1895
BstMBI GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
BstMCI CGRYCG 1 cut(s) 1831
BstNI CCWGG 5 cut(s) 859, 1111, 1372, 1618, 2086
BstNSI RCATGY 3 cut(s) 1477, 1520, 1960
BstPI GGTNACC 1 cut(s) 1720
BstSCI CCNGG 6 cut(s) 857, 1109, 1370, 1616, 2084, 2151
BstSFI CTRYAG 3 cut(s) 1236, 1328, 2122
BstUI CGCG 1 cut(s) 1183
BstV1I GCAGC 8 cut(s) 85, 139, 691, 1222, 1342, 1528, 1621, 2136
BstV2I GAAGAC 1 cut(s) 1859
BstX2I RGATCY 3 cut(s) 568, 1004, 1410
BstYI RGATCY 3 cut(s) 568, 1004, 1410
Bsu36I CCTNAGG 1 cut(s) 1599
BsuI GTATCC 2 cut(s) 591, 1705
BsuRI GGCC 4 cut(s) 313, 529, 1142, 1656
BtgI CCRYGG 3 cut(s) 159, 1218, 1570
BtrI CACGTC 2 cut(s) 853, 1753
BtsCI GGATG 4 cut(s) 14, 440, 867, 2185
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 270, 654
BtuMI TCGCGA 1 cut(s) 1183
BveI ACCTGC 3 cut(s) 1854, 2129, 2187
Cac8I GCNNGC 8 cut(s) 84, 178, 756, 1181, 1438, 1479, 1518, 1522
CaiI CAGNNNCTG 1 cut(s) 2101
CciI TCATGA 1 cut(s) 1393
CfoI GCGC 2 cut(s) 118, 1368
Cfr13I GGNCC 3 cut(s) 817, 1594, 1655
Csp6I GTAC 6 cut(s) 100, 127, 442, 547, 1534, 2104
CspCI CAANNNNNGTGG 2 cut(s) 1866, 1901
CviQI GTAC 6 cut(s) 100, 127, 442, 547, 1534, 2104
DdeI CTNAG 9 cut(s) 318, 370, 381, 572, 696, 969, 1008, 1599, 2071
DpnI GATC 7 cut(s) 269, 570, 1006, 1044, 1412, 1673, 2112
DpnII GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
DrdI GACNNNNNNGTC 2 cut(s) 331, 964
DseDI GACNNNNNNGTC 2 cut(s) 331, 964
Eam1104I CTCTTC 6 cut(s) 190, 393, 411, 606, 615, 1776
EarI CTCTTC 6 cut(s) 190, 393, 411, 606, 615, 1776
EciI GGCGGA 4 cut(s) 260, 568, 684, 705
Ecl136II GAGCTC 2 cut(s) 558, 1825
Eco130I CCWWGG 2 cut(s) 1218, 2175
Eco24I GRGCYC 3 cut(s) 560, 1762, 1827
Eco31I GGTCTC 1 cut(s) 1208
Eco47I GGWCC 2 cut(s) 817, 1594
Eco53kI GAGCTC 2 cut(s) 558, 1825
Eco57I CTGAAG 5 cut(s) 98, 287, 798, 1472, 1490
Eco72I CACGTG 1 cut(s) 353
Eco81I CCTNAGG 1 cut(s) 1599
Eco88I CYCGRG 1 cut(s) 1871
Eco91I GGTNACC 1 cut(s) 1720
EcoICRI GAGCTC 2 cut(s) 558, 1825
EcoNI CCTNNNNNAGG 1 cut(s) 1418
EcoO65I GGTNACC 1 cut(s) 1720
EcoRI GAATTC 1 cut(s) 1836
EcoRII CCWGG 5 cut(s) 857, 1109, 1370, 1616, 2084
EcoT14I CCWWGG 2 cut(s) 1218, 2175
EcoT38I GRGCYC 3 cut(s) 560, 1762, 1827
ErhI CCWWGG 2 cut(s) 1218, 2175
FaqI GGGAC 1 cut(s) 1580
FauI CCCGC 1 cut(s) 536
FauNDI CATATG 1 cut(s) 1555
FblI GTMKAC 1 cut(s) 323
FokI GGATG 4 cut(s) 21, 447, 874, 2192
FriOI GRGCYC 3 cut(s) 560, 1762, 1827
FspAI RTGCGCAY 1 cut(s) 1367
FspBI CTAG 3 cut(s) 608, 828, 1814
FspI TGCGCA 1 cut(s) 1367
GlaI GCGC 2 cut(s) 117, 1367
GsaI CCCAGC 2 cut(s) 29, 2148
GsuI CTGGAG 2 cut(s) 1600, 2068
HaeIII GGCC 4 cut(s) 313, 529, 1142, 1656
HapII CCGG 5 cut(s) 233, 530, 1143, 1709, 2153
HhaI GCGC 2 cut(s) 118, 1368
Hin6I GCGC 2 cut(s) 116, 1366
HinP1I GCGC 2 cut(s) 116, 1366
HincII GTYRAC 3 cut(s) 324, 1020, 1807
HindII GTYRAC 3 cut(s) 324, 1020, 1807
HindIII AAGCTT 2 cut(s) 971, 1738
HpaII CCGG 5 cut(s) 233, 530, 1143, 1709, 2153
HphI GGTGA 6 cut(s) 210, 468, 592, 715, 1435, 1732
Hpy166II GTNNAC 6 cut(s) 163, 324, 817, 1020, 1807, 1975
Hpy8I GTNNAC 6 cut(s) 163, 324, 817, 1020, 1807, 1975
Hpy99I CGWCG 2 cut(s) 566, 1541
HpyAV CCTTC 7 cut(s) 29, 311, 587, 700, 773, 799, 2159
HpyCH4III ACNGT 2 cut(s) 1574, 2103
HpyCH4IV ACGT 7 cut(s) 352, 365, 852, 1536, 1752, 2004, 2034
HpyF3I CTNAG 9 cut(s) 318, 370, 381, 572, 696, 969, 1008, 1599, 2071
HpySE526I ACGT 7 cut(s) 352, 365, 852, 1536, 1752, 2004, 2034
HspAI GCGC 2 cut(s) 116, 1366
Kzo9I GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
LguI GCTCTTC 1 cut(s) 1776
LmnI GCTCC 9 cut(s) 262, 555, 563, 692, 742, 787, 1620, 1642, 1945
Lsp1109I GCAGC 8 cut(s) 85, 139, 691, 1222, 1342, 1528, 1621, 2136
LweI GCATC 4 cut(s) 508, 852, 859, 1007
MaeI CTAG 3 cut(s) 608, 828, 1814
MaeII ACGT 7 cut(s) 352, 365, 852, 1536, 1752, 2004, 2034
MaeIII GTNAC 6 cut(s) 46, 344, 474, 1720, 2023, 2116
MalI GATC 7 cut(s) 269, 570, 1006, 1044, 1412, 1673, 2112
MboI GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
MflI RGATCY 3 cut(s) 568, 1004, 1410
MhlI GDGCHC 3 cut(s) 560, 1762, 1827
MlyI GAGTC 8 cut(s) 329, 341, 368, 637, 997, 1295, 1934, 2075
MmeI TCCRAC 3 cut(s) 585, 774, 1365
MroXI GAANNNNTTC 2 cut(s) 1069, 1081
MseI TTAA 9 cut(s) 909, 1035, 1137, 1188, 1265, 1361, 1584, 1650, 2030
MslI CAYNNNNRTG 3 cut(s) 351, 435, 1476
MspA1I CMGCKG 3 cut(s) 76, 776, 1634
MspI CCGG 5 cut(s) 233, 530, 1143, 1709, 2153
MspR9I CCNGG 6 cut(s) 859, 1111, 1372, 1618, 2086, 2153
Mva1269I GAATGC 1 cut(s) 1844
MvaI CCWGG 5 cut(s) 859, 1111, 1372, 1618, 2086
MvnI CGCG 1 cut(s) 1183
NciI CCSGG 1 cut(s) 2153
NcoI CCATGG 1 cut(s) 1218
NdeI CATATG 1 cut(s) 1555
NdeII GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
NlaIV GGNNCC 5 cut(s) 258, 1596, 1657, 2150, 2173
NmeAIII GCCGAG 1 cut(s) 1309
NmuCI GTSAC 6 cut(s) 46, 344, 474, 1720, 2023, 2116
NruI TCGCGA 1 cut(s) 1183
NsbI TGCGCA 1 cut(s) 1367
NspI RCATGY 3 cut(s) 1477, 1520, 1960
OliI CACNNNNGTG 2 cut(s) 351, 1476
PaeI GCATGC 1 cut(s) 1520
PaeR7I CTCGAG 1 cut(s) 1871
PagI TCATGA 1 cut(s) 1393
PaqCI CACCTGC 1 cut(s) 2187
PciI ACATGT 1 cut(s) 1956
PciSI GCTCTTC 1 cut(s) 1776
PcsI WCGNNNNNNNCGW 2 cut(s) 962, 2112
PctI GAATGC 1 cut(s) 1844
PdmI GAANNNNTTC 2 cut(s) 1069, 1081
PfeI GAWTC 6 cut(s) 839, 1070, 1260, 1302, 1388, 1768
PflFI GACNNNGTC 1 cut(s) 1924
PfoI TCCNGGA 2 cut(s) 857, 1616
PleI GAGTC 8 cut(s) 328, 340, 368, 636, 996, 1294, 1933, 2074
PmaCI CACGTG 1 cut(s) 353
PmlI CACGTG 1 cut(s) 353
PpsI GAGTC 8 cut(s) 328, 340, 368, 636, 996, 1294, 1933, 2074
Ppu21I YACGTR 2 cut(s) 353, 2005
PscI ACATGT 1 cut(s) 1956
PsiI TTATAA 1 cut(s) 539
Psp124BI GAGCTC 2 cut(s) 560, 1827
Psp6I CCWGG 5 cut(s) 857, 1109, 1370, 1616, 2084
PspCI CACGTG 1 cut(s) 353
PspEI GGTNACC 1 cut(s) 1720
PspFI CCCAGC 2 cut(s) 25, 2144
PspGI CCWGG 5 cut(s) 857, 1109, 1370, 1616, 2084
PspN4I GGNNCC 5 cut(s) 258, 1596, 1657, 2150, 2173
PspPI GGNCC 3 cut(s) 817, 1594, 1655
PsrI GAACNNNNNNTAC 2 cut(s) 1439, 1471
PstI CTGCAG 3 cut(s) 1240, 1332, 2126
PstNI CAGNNNCTG 1 cut(s) 2101
PsuI RGATCY 3 cut(s) 568, 1004, 1410
PsyI GACNNNGTC 1 cut(s) 1924
PvuII CAGCTG 3 cut(s) 76, 776, 1634
RruI TCGCGA 1 cut(s) 1183
RsaI GTAC 6 cut(s) 101, 128, 443, 548, 1535, 2105
RsaNI GTAC 6 cut(s) 100, 127, 442, 547, 1534, 2104
RseI CAYNNNNRTG 3 cut(s) 351, 435, 1476
SacI GAGCTC 2 cut(s) 560, 1827
SalI GTCGAC 1 cut(s) 322
SapI GCTCTTC 1 cut(s) 1776
SaqAI TTAA 9 cut(s) 909, 1035, 1137, 1188, 1265, 1361, 1584, 1650, 2030
Sau3AI GATC 7 cut(s) 267, 568, 1004, 1042, 1410, 1671, 2110
Sau96I GGNCC 3 cut(s) 817, 1594, 1655
ScaI AGTACT 1 cut(s) 128
SchI GAGTC 8 cut(s) 329, 341, 368, 637, 997, 1295, 1934, 2075
ScrFI CCNGG 6 cut(s) 859, 1111, 1372, 1618, 2086, 2153
SduI GDGCHC 3 cut(s) 560, 1762, 1827
SfaNI GCATC 4 cut(s) 508, 852, 859, 1007
SfcI CTRYAG 3 cut(s) 1236, 1328, 2122
Sfr274I CTCGAG 1 cut(s) 1871
SinI GGWCC 2 cut(s) 817, 1594
SlaI CTCGAG 1 cut(s) 1871
SmiMI CAYNNNNRTG 3 cut(s) 351, 435, 1476
SmlI CTYRAG 3 cut(s) 1230, 1732, 1871
SmoI CTYRAG 3 cut(s) 1230, 1732, 1871
SphI GCATGC 1 cut(s) 1520
SspMI CTAG 3 cut(s) 608, 828, 1814
SstI GAGCTC 2 cut(s) 560, 1827
StyD4I CCNGG 6 cut(s) 857, 1109, 1370, 1616, 2084, 2151
StyI CCWWGG 2 cut(s) 1218, 2175
TaaI ACNGT 2 cut(s) 1574, 2103
TaiI ACGT 7 cut(s) 355, 368, 855, 1539, 1755, 2007, 2037
TaqII GACCGA 2 cut(s) 1845, 1937
TatI WGTACW 1 cut(s) 126
TauI GCSGC 5 cut(s) 73, 158, 239, 257, 2172
TfiI GAWTC 6 cut(s) 839, 1070, 1260, 1302, 1388, 1768
Tru1I TTAA 9 cut(s) 909, 1035, 1137, 1188, 1265, 1361, 1584, 1650, 2030
Tru9I TTAA 9 cut(s) 909, 1035, 1137, 1188, 1265, 1361, 1584, 1650, 2030
TscAI CASTG 2 cut(s) 277, 654
TseFI GTSAC 6 cut(s) 46, 344, 474, 1720, 2023, 2116
TseI GCWGC 9 cut(s) 73, 152, 679, 1235, 1330, 1541, 1634, 2124, 2193
Tsp45I GTSAC 6 cut(s) 46, 344, 474, 1720, 2023, 2116
TspDTI ATGAA 3 cut(s) 951, 1338, 1410
TspGWI ACGGA 4 cut(s) 1043, 1171, 1629, 1813
TspRI CASTG 2 cut(s) 277, 654
Tth111I GACNNNGTC 1 cut(s) 1924
VpaK11BI GGWCC 2 cut(s) 817, 1594
XagI CCTNNNNNAGG 1 cut(s) 1418
XapI RAATTY 2 cut(s) 1031, 1836
XceI RCATGY 3 cut(s) 1477, 1520, 1960
XhoI CTCGAG 1 cut(s) 1871
XmiI GTMKAC 1 cut(s) 323
XmnI GAANNNNTTC 2 cut(s) 1069, 1081
XspI CTAG 3 cut(s) 608, 828, 1814
ZrmI AGTACT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.