MD05G1157000.v1.1

D-amino acid dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010015
Physical Location & Seq
Reverse (-)
28649441 .. 28660698
11258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1157000.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGGCTGCAGCTTCGACTTTTCTCTCGCACCCGAAGCCCCACACAAACGACGCCGTCTCGTCTTTACGAAGTCACTTCAAGCCATCCAGCTTCGCCTCCCAGTCAACCTCCTTCGGCTCCTCCAAATTCGCATCCAAATCGCTCTCCTTGCGCACCGCCGCAACCCGATCCGGCCGACCCGAACCCGCCACCTTGTCCCATTCGGAATCCCGGTCTGATCAAACATTCGATGTTTTAGTAATCGGAGCTGGAATAATCGGGTTGACCATTGCCCGGCAGTTCCTAATCGGGTCGGACCTGTCGGTCGCCGTAATTGACAAGTCTGTCCCTTGCTCTGGCGCCACCGGTGCCGGCCAAGGGTATATATGGATGGGACACAAAACTCCCGGCAGCGACCTTTGGGATTTGGGGCTCAGAAGCCAGAAGCTTTGGGAGGAATTGGCTGAGAGTTTGATTGAGCAAGGTTTGGACCCTCTGCAACTATTGGGTTGGAAGAAAACAGGAAGCTTGCTAGTTGGTAGAACTCCTGAGGACTTAGACGTGTTGAAAAGGAGGGTTAAGCAGGTATGTGAAGCCGGGTTAAGAGCGGAGTACTTGTCTTCCAGTGACTTGCATGTAAAGGAACCTGAACTCTTGGCTGATAAAGACACTGGAGCTGCCTTTCTACCTGATGATTGCCAGTTGGATGCACGCCGTGGTGTTGAATATCTTGAACAGGGTAACAGGAATTATGCATCGAAGGGTAGATATGCTGAGTTTTATAATCATCCGGTGATTAGTTTATTAAGATCTGGTGGCAATGGGGATGTTGTGGCTATTAAGACTTCTAGGAATACATTGCACTGCAAGAAGGCTATCGTAGTCGCAGCTGGTTGTTGGAGTGGGTCTTTGATGCGTGACCTGCTGAAAGAATCAGAAATTGTGCTGGATGTCCCCGTGAAACCACGAAAGGGTCACCTTTTAGTGCTTGAGAATTTTAATTCCTTTCATCTCAATCATGGGCTGATGGAGGTGGGATACGTTGATCATCAAACTGCAAATCCGCTTCCAAGCATATCGACTTCTGAATTGCTTAATCAAGATGAACAATCCTTGTCTGTGTCAATGACAGCCACTATGGATACAATGGGGAACATTATTCTTGGAAGCAGCCGCCAATTTGCTGGGTTCTGCACCGAGTTGGAAGAATCCATTATTAGCCGTATATGGGAGCGAGCTGGGGAGTTCTTTCCCAAATTAAAAGAAAAGCTCTTTTCAGATATCAGTAAGGGTAGAGAAGTGCGAGTAGGATTACGACCTTACATGCCAGATGGGAAGCCAGTGATTGGGCCTATGCCCGGTTTGGCAAACGTGTTCCTTGCAACTGGGCATGAAGGGGGAGGACTTTCCTTGGCTCTAGGGACTGCCGAAATGCTCACGGATATGGTGCTAGGAAATTCTGAGAAGGTTAACTCTGCACCGTTCACTGTTCATGGTCGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

495

Amino Acids

53.48

Weight (kDa)

6.55

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 77 - 475 5.7e-57 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
AasI GACNNNNNNGTC 2 cut(s) 302, 323
Acc16I TGCGCA 1 cut(s) 152
Acc36I ACCTGC 2 cut(s) 553, 909
AccB1I GGYRCC 2 cut(s) 338, 347
AccB7I CCANNNNNTGG 1 cut(s) 1325
AccBSI CCGCTC 1 cut(s) 587
AciI CCGC 6 cut(s) 156, 159, 186, 587, 1043, 1153
AclWI GGATC 1 cut(s) 162
AcoI YGGCCR 2 cut(s) 172, 352
AcsI RAATTY 3 cut(s) 125, 973, 1435
AcyI GRCGYC 2 cut(s) 51, 339
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 7 cut(s) 273, 335, 357, 950, 1207, 1325, 1337
AflIII ACRYGT 2 cut(s) 540, 1350
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 4 cut(s) 79, 547, 704, 713
AjiI CACGTC 1 cut(s) 541
AluBI AGCT 9 cut(s) 11, 90, 248, 427, 507, 656, 869, 1217, 1249
AluI AGCT 9 cut(s) 11, 90, 248, 427, 507, 656, 869, 1217, 1249
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 1 cut(s) 162
AoxI GGCC 3 cut(s) 172, 352, 1328
ApeKI GCWGC 6 cut(s) 5, 8, 390, 656, 866, 1149
ApoI RAATTY 3 cut(s) 125, 973, 1435
AsiGI ACCGGT 1 cut(s) 344
AspLEI GCGC 2 cut(s) 153, 341
AspS9I GGNCC 3 cut(s) 295, 469, 1328
AsuC2I CCSGG 5 cut(s) 211, 274, 387, 577, 1338
AsuHPI GGTGA 2 cut(s) 784, 947
AvaII GGWCC 2 cut(s) 295, 469
AxyI CCTNAGG 1 cut(s) 528
BanI GGYRCC 2 cut(s) 338, 347
BanII GRGCYC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 591
BbvI GCAGC 5 cut(s) 20, 402, 643, 878, 1161
BccI CCATC 4 cut(s) 91, 364, 1000, 1304
BceAI ACGGC 4 cut(s) 38, 293, 678, 1185
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciVI GTATCC 2 cut(s) 1010, 1114
BclI TGATCA 2 cut(s) 217, 1024
BcnI CCSGG 5 cut(s) 211, 274, 387, 577, 1338
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 4 cut(s) 512, 828, 1397, 1430
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 342
BfuAI ACCTGC 2 cut(s) 553, 909
BfuI GTATCC 2 cut(s) 1010, 1114
BglII AGATCT 1 cut(s) 788
BisI GCNGC 8 cut(s) 6, 9, 159, 391, 657, 867, 1150, 1153
BlsI GCNGC 8 cut(s) 7, 10, 160, 392, 658, 868, 1151, 1154
BmcAI AGTACT 1 cut(s) 593
Bme1390I CCNGG 5 cut(s) 211, 274, 387, 577, 1338
Bme18I GGWCC 2 cut(s) 295, 469
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 3 cut(s) 295, 469, 1328
BmiI GGNNCC 5 cut(s) 118, 340, 349, 471, 624
BmrFI CCNGG 5 cut(s) 211, 274, 387, 577, 1338
BmrI ACTGGG 2 cut(s) 94, 1374
BmsI GCATC 4 cut(s) 140, 676, 743, 882
BmuI ACTGGG 2 cut(s) 94, 1374
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 989
BpuMI CCSGG 5 cut(s) 211, 274, 387, 577, 1338
BsaHI GRCGYC 2 cut(s) 51, 339
BsaJI CCNNGG 3 cut(s) 355, 694, 1389
BsaWI WCCGGW 2 cut(s) 344, 769
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 7 cut(s) 273, 335, 357, 950, 1207, 1325, 1337
Bse118I RCCGGY 2 cut(s) 344, 350
Bse1I ACTGG 6 cut(s) 100, 603, 655, 679, 1319, 1369
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 267, 805, 836
BseDI CCNNGG 3 cut(s) 355, 694, 1389
BseGI GGATG 7 cut(s) 83, 131, 375, 691, 766, 811, 934
BseLI CCNNNNNNNGG 7 cut(s) 273, 335, 357, 950, 1207, 1325, 1337
BseMI GCAATG 3 cut(s) 267, 805, 836
BseMII CTCAG 5 cut(s) 427, 435, 519, 744, 1431
BseNI ACTGG 6 cut(s) 100, 603, 655, 679, 1319, 1369
BseRI GAGGAG 1 cut(s) 109
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 5 cut(s) 20, 402, 643, 878, 1161
BseYI CCCAGC 2 cut(s) 1163, 1217
BsgI GTGCAG 2 cut(s) 1156, 1440
Bsh1285I CGRYCG 2 cut(s) 175, 306
BshFI GGCC 3 cut(s) 174, 354, 1330
BshNI GGYRCC 2 cut(s) 338, 347
BshTI ACCGGT 1 cut(s) 344
BsiEI CGRYCG 2 cut(s) 175, 306
BsiSI CCGG 9 cut(s) 171, 211, 274, 345, 351, 387, 576, 770, 1338
BslFI GGGAC 5 cut(s) 181, 311, 387, 917, 1414
BslI CCNNNNNNNGG 7 cut(s) 273, 335, 357, 950, 1207, 1325, 1337
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 5 cut(s) 181, 311, 387, 917, 1414
BsnI GGCC 3 cut(s) 174, 354, 1330
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 4 cut(s) 167, 217, 788, 1024
BspACI CCGC 6 cut(s) 156, 159, 186, 587, 1043, 1153
BspANI GGCC 3 cut(s) 174, 354, 1330
BspCNI CTCAG 5 cut(s) 426, 436, 520, 745, 1432
BspLI GGNNCC 5 cut(s) 118, 340, 349, 471, 624
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 2 cut(s) 553, 909
BspPI GGATC 1 cut(s) 162
BspT107I GGYRCC 2 cut(s) 338, 347
BsrBI CCGCTC 1 cut(s) 587
BsrDI GCAATG 3 cut(s) 267, 805, 836
BsrFI RCCGGY 2 cut(s) 344, 350
BsrI ACTGG 6 cut(s) 100, 603, 655, 679, 1319, 1369
BssAI RCCGGY 2 cut(s) 344, 350
BssECI CCNNGG 3 cut(s) 355, 694, 1389
BssMI GATC 4 cut(s) 167, 217, 788, 1024
BssNI GRCGYC 2 cut(s) 51, 339
BssT1I CCWWGG 2 cut(s) 355, 1389
Bst4CI ACNGT 2 cut(s) 1461, 1468
BstACI GRCGYC 2 cut(s) 51, 339
BstC8I GCNNGC 4 cut(s) 352, 509, 691, 1215
BstDEI CTNAG 6 cut(s) 413, 444, 528, 535, 753, 1440
BstDSI CCRYGG 1 cut(s) 694
BstEII GGTNACC 1 cut(s) 953
BstF5I GGATG 7 cut(s) 83, 131, 375, 691, 766, 811, 934
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 2 cut(s) 153, 341
BstKTI GATC 4 cut(s) 170, 220, 791, 1027
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 4 cut(s) 167, 217, 788, 1024
BstMCI CGRYCG 2 cut(s) 175, 306
BstMWI GCNNNNNNNGC 4 cut(s) 34, 148, 347, 901
BstNSI RCATGY 2 cut(s) 617, 1306
BstPI GGTNACC 1 cut(s) 953
BstSCI CCNGG 5 cut(s) 209, 272, 385, 575, 1336
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 5 cut(s) 20, 402, 643, 878, 1161
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 1 cut(s) 788
BstXI CCANNNNNNTGG 1 cut(s) 1163
BstYI RGATCY 1 cut(s) 788
BstZI CGGCCG 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 2 cut(s) 1010, 1114
BsuRI GGCC 3 cut(s) 174, 354, 1330
BtgI CCRYGG 1 cut(s) 694
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 7 cut(s) 83, 131, 375, 691, 766, 811, 934
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 5 cut(s) 610, 648, 841, 1326, 1464
BveI ACCTGC 2 cut(s) 553, 909
Cac8I GCNNGC 4 cut(s) 352, 509, 691, 1215
CfoI GCGC 2 cut(s) 153, 341
Cfr10I RCCGGY 2 cut(s) 344, 350
Cfr13I GGNCC 3 cut(s) 295, 469, 1328
CseI GACGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 592
CspAI ACCGGT 1 cut(s) 344
CviAII CATG 5 cut(s) 614, 998, 1303, 1370, 1472
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 6 cut(s) 413, 444, 528, 535, 753, 1440
DinI GGCGCC 1 cut(s) 340
DpnI GATC 4 cut(s) 169, 219, 790, 1026
DpnII GATC 4 cut(s) 167, 217, 788, 1024
DraIII CACNNNGTG 1 cut(s) 695
DrdI GACNNNNNNGTC 2 cut(s) 302, 323
DseDI GACNNNNNNGTC 2 cut(s) 302, 323
EaeI YGGCCR 2 cut(s) 172, 352
EagI CGGCCG 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 355, 1389
Eco24I GRGCYC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 1261
Eco47I GGWCC 2 cut(s) 295, 469
Eco52I CGGCCG 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 953
EcoO65I GGTNACC 1 cut(s) 953
EcoRV GATATC 1 cut(s) 1261
EcoT14I CCWWGG 2 cut(s) 355, 1389
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 355, 1389
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 5 cut(s) 617, 1001, 1306, 1373, 1475
FaqI GGGAC 5 cut(s) 181, 311, 387, 917, 1414
FatI CATG 5 cut(s) 613, 997, 1302, 1369, 1471
FauI CCCGC 1 cut(s) 193
FbaI TGATCA 2 cut(s) 217, 1024
Fnu4HI GCNGC 8 cut(s) 6, 9, 159, 391, 657, 867, 1150, 1153
FokI GGATG 7 cut(s) 70, 118, 382, 698, 753, 818, 941
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 8 cut(s) 6, 9, 159, 391, 657, 867, 1150, 1153
FspBI CTAG 4 cut(s) 512, 828, 1397, 1430
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 2 cut(s) 152, 340
GluI GCNGC 8 cut(s) 6, 9, 159, 391, 657, 867, 1150, 1153
GsaI CCCAGC 2 cut(s) 1167, 1221
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 3 cut(s) 174, 354, 1330
HapII CCGG 9 cut(s) 171, 211, 274, 345, 351, 387, 576, 770, 1338
HgaI GACGC 1 cut(s) 59
HhaI GCGC 2 cut(s) 153, 341
Hin1I GRCGYC 2 cut(s) 51, 339
Hin1II CATG 5 cut(s) 617, 1001, 1306, 1373, 1475
Hin6I GCGC 2 cut(s) 151, 339
HinP1I GCGC 2 cut(s) 151, 339
HincII GTYRAC 3 cut(s) 105, 264, 1450
HindII GTYRAC 3 cut(s) 105, 264, 1450
HindIII AAGCTT 2 cut(s) 425, 505
HinfI GANTC 3 cut(s) 206, 911, 1187
HpaI GTTAAC 1 cut(s) 1450
HpaII CCGG 9 cut(s) 171, 211, 274, 345, 351, 387, 576, 770, 1338
HphI GGTGA 2 cut(s) 784, 947
Hpy166II GTNNAC 4 cut(s) 105, 264, 1450, 1464
Hpy188I TCNGA 9 cut(s) 205, 217, 245, 295, 416, 916, 1066, 1258, 1441
Hpy188III TCNNGA 3 cut(s) 527, 710, 1079
Hpy8I GTNNAC 4 cut(s) 105, 264, 1450, 1464
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 5 cut(s) 121, 733, 844, 1367, 1438
HpyCH4III ACNGT 2 cut(s) 1461, 1468
HpyCH4IV ACGT 3 cut(s) 540, 1020, 1350
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 148, 347, 901
HpyF3I CTNAG 6 cut(s) 413, 444, 528, 535, 753, 1440
HpySE526I ACGT 3 cut(s) 540, 1020, 1350
Hsp92I GRCGYC 2 cut(s) 51, 339
Hsp92II CATG 5 cut(s) 617, 1001, 1306, 1373, 1475
HspAI GCGC 2 cut(s) 151, 339
KasI GGCGCC 1 cut(s) 338
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Ksp22I TGATCA 2 cut(s) 217, 1024
KspAI GTTAAC 1 cut(s) 1450
Kzo9I GATC 4 cut(s) 167, 217, 788, 1024
LmnI GCTCC 4 cut(s) 122, 245, 653, 1210
Lsp1109I GCAGC 5 cut(s) 20, 402, 643, 878, 1161
LweI GCATC 4 cut(s) 140, 676, 743, 882
MaeI CTAG 4 cut(s) 512, 828, 1397, 1430
MaeII ACGT 3 cut(s) 540, 1020, 1350
MaeIII GTNAC 5 cut(s) 71, 605, 719, 896, 953
MalI GATC 4 cut(s) 169, 219, 790, 1026
MbiI CCGCTC 1 cut(s) 587
MboI GATC 4 cut(s) 167, 217, 788, 1024
MboII GAAGA 3 cut(s) 505, 591, 1196
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 1 cut(s) 414
Mly113I GGCGCC 1 cut(s) 339
MmeI TCCRAC 5 cut(s) 273, 470, 663, 857, 1161
MnlI CCTC 9 cut(s) 106, 118, 130, 427, 483, 523, 546, 1003, 1373
Mph1103I ATGCAT 1 cut(s) 736
MroNI GCCGGC 1 cut(s) 350
MseI TTAA 8 cut(s) 558, 581, 785, 819, 978, 1074, 1238, 1449
MslI CAYNNNNRTG 1 cut(s) 1421
MspA1I CMGCKG 1 cut(s) 869
MspI CCGG 9 cut(s) 171, 211, 274, 345, 351, 387, 576, 770, 1338
MspR9I CCNGG 5 cut(s) 211, 274, 387, 577, 1338
MwoI GCNNNNNNNGC 4 cut(s) 34, 148, 347, 901
NaeI GCCGGC 1 cut(s) 352
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 5 cut(s) 211, 274, 387, 577, 1338
NdeII GATC 4 cut(s) 167, 217, 788, 1024
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 5 cut(s) 617, 1001, 1306, 1373, 1475
NlaIV GGNNCC 5 cut(s) 118, 340, 349, 471, 624
NmuCI GTSAC 4 cut(s) 71, 605, 896, 953
NsbI TGCGCA 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 2 cut(s) 617, 1306
PdiI GCCGGC 1 cut(s) 352
PfeI GAWTC 3 cut(s) 206, 911, 1187
PflFI GACNNNGTC 1 cut(s) 53
PflMI CCANNNNNTGG 1 cut(s) 1325
PinAI ACCGGT 1 cut(s) 344
PkrI GCNGC 8 cut(s) 7, 10, 160, 392, 658, 868, 1151, 1154
PluTI GGCGCC 1 cut(s) 342
PsiI TTATAA 1 cut(s) 762
PspEI GGTNACC 1 cut(s) 953
PspFI CCCAGC 2 cut(s) 1163, 1217
PspN4I GGNNCC 5 cut(s) 118, 340, 349, 471, 624
PspPI GGNCC 3 cut(s) 295, 469, 1328
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 788
PsyI GACNNNGTC 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 1 cut(s) 1421
SaqAI TTAA 8 cut(s) 558, 581, 785, 819, 978, 1074, 1238, 1449
SatI GCNGC 8 cut(s) 6, 9, 159, 391, 657, 867, 1150, 1153
Sau3AI GATC 4 cut(s) 167, 217, 788, 1024
Sau96I GGNCC 3 cut(s) 295, 469, 1328
ScaI AGTACT 1 cut(s) 593
ScrFI CCNGG 5 cut(s) 211, 274, 387, 577, 1338
SduI GDGCHC 1 cut(s) 414
SfaNI GCATC 4 cut(s) 140, 676, 743, 882
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 340
SgrAI CRCCGGYG 1 cut(s) 344
SinI GGWCC 2 cut(s) 295, 469
SmiMI CAYNNNNRTG 1 cut(s) 1421
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
SsiI CCGC 6 cut(s) 156, 159, 186, 587, 1043, 1153
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 4 cut(s) 512, 828, 1397, 1430
StyD4I CCNGG 5 cut(s) 209, 272, 385, 575, 1336
StyI CCWWGG 2 cut(s) 355, 1389
TaaI ACNGT 2 cut(s) 1461, 1468
TaiI ACGT 3 cut(s) 543, 1023, 1353
TaqI TCGA 5 cut(s) 14, 228, 737, 1058, 1477
TaqII GACCGA 1 cut(s) 292
TatI WGTACW 1 cut(s) 591
TauI GCSGC 2 cut(s) 161, 1155
TfiI GAWTC 3 cut(s) 206, 911, 1187
Tru1I TTAA 8 cut(s) 558, 581, 785, 819, 978, 1074, 1238, 1449
Tru9I TTAA 8 cut(s) 558, 581, 785, 819, 978, 1074, 1238, 1449
TscAI CASTG 5 cut(s) 610, 655, 848, 1326, 1471
TseFI GTSAC 4 cut(s) 71, 605, 896, 953
TseI GCWGC 6 cut(s) 5, 8, 390, 656, 866, 1149
Tsp45I GTSAC 4 cut(s) 71, 605, 896, 953
TspDTI ATGAA 4 cut(s) 977, 1098, 1386, 1460
TspGWI ACGGA 1 cut(s) 1433
TspRI CASTG 5 cut(s) 610, 655, 848, 1326, 1471
Tth111I GACNNNGTC 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 1325
VpaK11BI GGWCC 2 cut(s) 295, 469
XapI RAATTY 3 cut(s) 125, 973, 1435
XceI RCATGY 2 cut(s) 617, 1306
XspI CTAG 4 cut(s) 512, 828, 1397, 1430
ZrmI AGTACT 1 cut(s) 593
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.