Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002498

Physical Location & Seq

Genomic Coordinates Reverse (-)

30263157 .. 30267636

Gene Structure 4480 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1174600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGCCAGGAGAAGTAATTTCGTTGCAAGAAGACGAAAAGACTAGAACTTTTGATTTCGAAACCTCCGAGGACGAGAGGCGGAGACCTCGGAGCAGATCTCTTAAGAAAAGGACGACAAATGCTTCCTTGAGATTAACAAACACTCCTAGAAAGCGTGGCACGCGTGTGGCCAATTGTCGCTACGCATCGATTTCAATTGAAAATGCTCGGGATGCTGAGGAGGAAGAAGCTGTGATTACGTTTCGCGATGCATTGGTTGCTAAAGATATGCTCCCGGCTCAATTCGATGATTATCATACAATGCTGAGATTTTTGAAGGCGAGGAAGTTTGACATTGATAAATCTTTTCAAATGTGGGCAGAAATGCTCAACTGGAGGAGAGAGTATGGAGTAGAATCTATTGTGAAGGATTTTGTGTACAGTGAATATGAAGAGGTTCAGCGTTTCTATCCTCATGGTTATCATGGTGTAGACAAGGCAGGGCGACCTCTTTATATTGAAAGACTTGGAAAAGTTGAACCTAGCAAGCTAATGAATGTCACCACAGTGGAACGGTTTCTAAAATATCATGTCCAGGGTTTTGAGAAGGCTTTTGCTGAGAAATTCCCAGCATGTTCTATTGCGGCCAAAAAGCATATAGATTCTACAACCACTATTCTAGATGTCCAGGGGATGAACTGGATGAGTTTTGGCAAGGTTGCACATGACCTTGTTATGCGCATGCAGAAAATGGACGGTGATAACTATCCTGAGACATTACATCAAATGTTTGTGGTAAATGCTGGTAATGGGTTCAAGCTACTTTGGAACACAGCAAAAGGCTTTCTTGACCCAAGGACTACTGGAAAGATACATGTCTTAGGCAACAGATTTCAAAACAAGCTTTTGGAAATCATAGACTTGAGCCAACTTCCGGATTTCCTTGGGGGAACTTGTTCGTGTCCAAATGAAGGCGGGTGCCTTAGGTCTGGAAAGGGACCCTGGAATGATCCAGAAATAATGAAACTGGTACACGTTGGAGAAACAATGCACCTGAGGAAAATCAAAGGTTCTGATGGTGACGATGTCGGAATCAAGCTGCTTACCTCTAAGATTACAGATAGTGAAATAGTATCTGCAGCTGAGTCAAGATCAGAAATGAGGTCAAGTACTTCTGCCTTCTTGCAGCTATCAAAGTCTAACTCTGACGAAGAGAGGGCAACTGAGTCTGCTGCATCTCCCTGCAGCCTAGTTGAATCAGGCAGTACTGCAGGAAGAATGGAAGTTGCTAACTCAAGTTTAAAAAGTGATTCACCCATTAACTTCACCCCAAGAAGGTCGCTGAAGACATCCATTCCACATGTAGCGAGTTTAACCGTTCACCTCATACTCAAAATATTAACAGGCATATATCTCGTATTCCCAATGTTAGGGAGGATATTTGCAGCATGTTATTCAGATAGCTGCTTAAAAACACGATTCAAACCTCTGCCAGTTGAAAATCCAAGTTCTCAGGAACAACAGAATTCACAGGGAATAGATGTAGGCCCCCTCTGGCAGAGGCTGCAGCAGTTAGAAGGATTGGTTACCAATTTGACAAACAAACCTACTAAAATTCCTCAAGAGAAAGAAGACATGCTTCACGAATCTTTAAATCGCATTAAATCTATAGAGTACGACTTACAGAAGACAAAGAAAGCATTGTTCGCAACTGCATCAAAGCAAGTGGAGCTTTCTAAGTCGCTGAAAAGTTTAAAAGATGACAGTTTAACCGGATCAAACTCATGTTGGCCAAGAAGTTGCCGTTACGACCACGACCCAGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

603

Amino Acids

68.08

Weight (kDa)

8.77

Isoelectric Point (pI)

48.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	98 - 120	5.6e-06	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	153 - 310	1.7e-27	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002498)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24840 AT3G24840 AT3G24840 AT3G24840 AT3G24840 AT3G24840 AT3G24840
fragaria_vesca	FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970 FvH4_2g18970
malus_domestica	MD05G1174600.v1.1 MD10G1163800.v1.1
prunus_persica	Prupe.8G202100_v2.0.a1 Prupe.8G202100_v2.0.a1 Prupe.8G202100_v2.0.a1 Prupe.8G202100_v2.0.a1 Prupe.8G202100_v2.0.a1 Prupe.8G202100_v2.0.a1
pyrus_communis	pycom05g16150 pycom10g14120
rosa_chinensis	RchiOBHm_Chr6g0284741
rosa_laevigata	RLG00000012722
rosa_multiflora	Rmu_sc0008596.1_g000005 Rmu_sc0022173.1_g000001
rosa_roxburghii	Rroxscaffold_7G00184420
rosa_rugosa	Rorug06G0166200 Rorug06G0166200 Rorug06G0166300
rosa_samantha	Rh6AG279200 Rh6BG279300 Rh6CG281500 Rh6DG274200
rosa_wichuraiana	Rw6G024000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	719
AccB1I	GGYRCC	1 cut(s)	957
AccI	GTMKAC	1 cut(s)	471
AccII	CGCG	2 cut(s)	163, 246
AccIII	TCCGGA	1 cut(s)	913
AciI	CCGC	3 cut(s)	79, 623, 954
AclWI	GGATC	2 cut(s)	983, 1762
AcoI	YGGCCR	3 cut(s)	168, 624, 1769
AcsI	RAATTY	3 cut(s)	602, 1504, 1593
AcuI	CTGAAG	1 cut(s)	1343
AfaI	GTAC	5 cut(s)	419, 1011, 1150, 1246, 1655
AfiI	CCNNNNNNNGG	4 cut(s)	913, 950, 1314, 1409
AflII	CTTAAG	1 cut(s)	101
AflIII	ACRYGT	4 cut(s)	161, 853, 1012, 1339
AjnI	CCWGG	5 cut(s)	4, 573, 666, 980, 1798
AleI	CACNNNNGTG	2 cut(s)	164, 545
AluBI	AGCT	9 cut(s)	230, 529, 799, 883, 1078, 1121, 1168, 1443, 1711
AluI	AGCT	9 cut(s)	230, 529, 799, 883, 1078, 1121, 1168, 1443, 1711
Alw26I	GTCTC	2 cut(s)	76, 746
AlwI	GGATC	2 cut(s)	983, 1762
AlwNI	CAGNNNCTG	1 cut(s)	1543
Ama87I	CYCGRG	1 cut(s)	207
Aor13HI	TCCGGA	1 cut(s)	913
AoxI	GGCC	4 cut(s)	168, 624, 1525, 1769
ApeKI	GCWGC	9 cut(s)	1078, 1118, 1165, 1211, 1224, 1424, 1443, 1543, 1546
ApoI	RAATTY	3 cut(s)	602, 1504, 1593
Asp700I	GAANNNNTTC	3 cut(s)	435, 555, 934
AspLEI	GCGC	1 cut(s)	720
AspS9I	GGNCC	2 cut(s)	977, 1526
AsuC2I	CCSGG	1 cut(s)	275
AsuHPI	GGTGA	6 cut(s)	532, 749, 1070, 1284, 1297, 1352
AsuII	TTCGAA	1 cut(s)	57
AvaI	CYCGRG	1 cut(s)	207
AvaII	GGWCC	1 cut(s)	977
AxyI	CCTNAGG	2 cut(s)	962, 1034
BalI	TGGCCA	2 cut(s)	170, 1771
BanI	GGYRCC	1 cut(s)	957
BbsI	GAAGAC	4 cut(s)	36, 1331, 1617, 1673
BbvCI	CCTCAGC	1 cut(s)	216
BbvI	GCAGC	9 cut(s)	1065, 1130, 1177, 1198, 1236, 1430, 1436, 1530, 1558
BccI	CCATC	1 cut(s)	1049
BceAI	ACGGC	1 cut(s)	1767
BcgI	CGANNNNNNTGC	2 cut(s)	1375, 1409
BciT130I	CCWGG	5 cut(s)	6, 575, 668, 982, 1800
BcnI	CCSGG	1 cut(s)	275
BcoDI	GTCTC	2 cut(s)	76, 746
BfaI	CTAG	5 cut(s)	42, 147, 522, 659, 1229
BfmI	CTRYAG	5 cut(s)	1116, 1222, 1248, 1544, 1647
BfrI	CTTAAG	1 cut(s)	101
BglII	AGATCT	1 cut(s)	95
BmcAI	AGTACT	2 cut(s)	1150, 1246
Bme1390I	CCNGG	6 cut(s)	6, 275, 575, 668, 982, 1800
Bme18I	GGWCC	1 cut(s)	977
BmeT110I	CYCGRG	1 cut(s)	207
BmgT120I	GGNCC	2 cut(s)	977, 1526
BmiI	GGNNCC	4 cut(s)	959, 978, 979, 1528
BmrFI	CCNGG	6 cut(s)	6, 275, 575, 668, 982, 1800
BmsI	GCATC	5 cut(s)	194, 202, 238, 1223, 1703
BpiI	GAAGAC	4 cut(s)	36, 1331, 1617, 1673
BplI	GAGNNNNNCTC	2 cut(s)	82, 114
BpmI	CTGGAG	1 cut(s)	394
Bpu10I	CCTNAGC	1 cut(s)	216
Bpu14I	TTCGAA	1 cut(s)	57
BpuEI	CTTGAG	4 cut(s)	148, 922, 1258, 1584
BpuMI	CCSGG	1 cut(s)	275
Bsa29I	ATCGAT	1 cut(s)	188
BsaBI	GATNNNNATC	2 cut(s)	291, 744
BsaI	GGTCTC	1 cut(s)	76
BsaJI	CCNNGG	8 cut(s)	66, 86, 574, 667, 833, 922, 980, 1798
BsaWI	WCCGGW	2 cut(s)	913, 1751
BsaXI	ACNNNNNCTCC	2 cut(s)	127, 157
Bsc4I	CCNNNNNNNGG	4 cut(s)	913, 950, 1314, 1409
Bse1I	ACTGG	5 cut(s)	377, 683, 847, 1011, 1472
Bse21I	CCTNAGG	2 cut(s)	962, 1034
Bse8I	GATNNNNATC	2 cut(s)	291, 744
BseAI	TCCGGA	1 cut(s)	913
BseBI	CCWGG	5 cut(s)	6, 575, 668, 982, 1800
BseCI	ATCGAT	1 cut(s)	188
BseDI	CCNNGG	8 cut(s)	66, 86, 574, 667, 833, 922, 980, 1798
BseGI	GGATG	4 cut(s)	217, 678, 687, 1328
BseJI	GATNNNNATC	2 cut(s)	291, 744
BseLI	CCNNNNNNNGG	4 cut(s)	913, 950, 1314, 1409
BseMII	CTCAG	8 cut(s)	207, 296, 588, 741, 1025, 1113, 1194, 1505
BseNI	ACTGG	5 cut(s)	377, 683, 847, 1011, 1472
BseRI	GAGGAG	2 cut(s)	233, 391
BseXI	GCAGC	9 cut(s)	1065, 1130, 1177, 1198, 1236, 1430, 1436, 1530, 1558
BseYI	CCCAGC	1 cut(s)	607
Bsh1236I	CGCG	2 cut(s)	163, 246
BshFI	GGCC	4 cut(s)	170, 626, 1527, 1771
BshNI	GGYRCC	1 cut(s)	957
BshVI	ATCGAT	1 cut(s)	188
BsiHKCI	CYCGRG	1 cut(s)	207
BsiSI	CCGG	3 cut(s)	275, 914, 1752
BslFI	GGGAC	1 cut(s)	990
BslI	CCNNNNNNNGG	4 cut(s)	913, 950, 1314, 1409
BsmAI	GTCTC	2 cut(s)	76, 746
BsmFI	GGGAC	1 cut(s)	990
BsnI	GGCC	4 cut(s)	170, 626, 1527, 1771
Bso31I	GGTCTC	1 cut(s)	76
BsoBI	CYCGRG	1 cut(s)	207
Bsp119I	TTCGAA	1 cut(s)	57
Bsp13I	TCCGGA	1 cut(s)	913
Bsp1407I	TGTACA	1 cut(s)	417
Bsp143I	GATC	4 cut(s)	95, 988, 1130, 1754
Bsp68I	TCGCGA	1 cut(s)	246
BspACI	CCGC	3 cut(s)	79, 623, 954
BspANI	GGCC	4 cut(s)	170, 626, 1527, 1771
BspCNI	CTCAG	8 cut(s)	208, 297, 589, 742, 1026, 1114, 1195, 1504
BspDI	ATCGAT	1 cut(s)	188
BspEI	TCCGGA	1 cut(s)	913
BspFNI	CGCG	2 cut(s)	163, 246
BspLI	GGNNCC	4 cut(s)	959, 978, 979, 1528
BspMAI	CTGCAG	4 cut(s)	1120, 1226, 1252, 1548
BspPI	GGATC	2 cut(s)	983, 1762
BspT104I	TTCGAA	1 cut(s)	57
BspT107I	GGYRCC	1 cut(s)	957
BspTI	CTTAAG	1 cut(s)	101
BspTNI	GGTCTC	1 cut(s)	76
BsrGI	TGTACA	1 cut(s)	417
BsrI	ACTGG	5 cut(s)	377, 683, 847, 1011, 1472
BssECI	CCNNGG	8 cut(s)	66, 86, 574, 667, 833, 922, 980, 1798
BssMI	GATC	4 cut(s)	95, 988, 1130, 1754
BssT1I	CCWWGG	2 cut(s)	833, 922
Bst2UI	CCWGG	5 cut(s)	6, 575, 668, 982, 1800
Bst4CI	ACNGT	6 cut(s)	422, 547, 555, 737, 1357, 1745
Bst6I	CTCTTC	2 cut(s)	426, 1185
BstAFI	CTTAAG	1 cut(s)	101
BstAPI	GCANNNNNTGC	2 cut(s)	257, 1543
BstAUI	TGTACA	1 cut(s)	417
BstBI	TTCGAA	1 cut(s)	57
BstC8I	GCNNGC	3 cut(s)	161, 527, 722
BstEII	GGTNACC	1 cut(s)	1564
BstF5I	GGATG	4 cut(s)	217, 678, 687, 1328
BstFNI	CGCG	2 cut(s)	163, 246
BstHHI	GCGC	1 cut(s)	720
BstKTI	GATC	4 cut(s)	98, 991, 1133, 1757
BstMAI	GTCTC	2 cut(s)	76, 746
BstMBI	GATC	4 cut(s)	95, 988, 1130, 1754
BstMWI	GCNNNNNNNGC	6 cut(s)	160, 212, 257, 1543, 1685, 1708
BstNI	CCWGG	5 cut(s)	6, 575, 668, 982, 1800
BstNSI	RCATGY	6 cut(s)	615, 724, 857, 1343, 1431, 1618
BstPI	GGTNACC	1 cut(s)	1564
BstSCI	CCNGG	6 cut(s)	4, 273, 573, 666, 980, 1798
BstSFI	CTRYAG	5 cut(s)	1116, 1222, 1248, 1544, 1647
BstUI	CGCG	2 cut(s)	163, 246
BstV1I	GCAGC	9 cut(s)	1065, 1130, 1177, 1198, 1236, 1430, 1436, 1530, 1558
BstV2I	GAAGAC	4 cut(s)	36, 1331, 1617, 1673
BstX2I	RGATCY	1 cut(s)	95
BstYI	RGATCY	1 cut(s)	95
Bsu15I	ATCGAT	1 cut(s)	188
Bsu36I	CCTNAGG	2 cut(s)	962, 1034
BsuRI	GGCC	4 cut(s)	170, 626, 1527, 1771
BsuTUI	ATCGAT	1 cut(s)	188
BtgZI	GCGATG	1 cut(s)	261
BtsCI	GGATG	4 cut(s)	217, 678, 687, 1328
BtsIMutI	CAGTG	2 cut(s)	427, 552
BtuMI	TCGCGA	1 cut(s)	246
Cac8I	GCNNGC	3 cut(s)	161, 527, 722
CaiI	CAGNNNCTG	1 cut(s)	1543
CfoI	GCGC	1 cut(s)	720
Cfr13I	GGNCC	2 cut(s)	977, 1526
ClaI	ATCGAT	1 cut(s)	188
Csp6I	GTAC	5 cut(s)	418, 1010, 1149, 1245, 1654
CspCI	CAANNNNNGTGG	2 cut(s)	1686, 1721
CviQI	GTAC	5 cut(s)	418, 1010, 1149, 1245, 1654
DpnI	GATC	4 cut(s)	97, 990, 1132, 1756
DpnII	GATC	4 cut(s)	95, 988, 1130, 1754
DraI	TTTAAA	3 cut(s)	1281, 1632, 1734
EaeI	YGGCCR	3 cut(s)	168, 624, 1769
Eam1104I	CTCTTC	2 cut(s)	426, 1185
EarI	CTCTTC	2 cut(s)	426, 1185
EciI	GGCGGA	1 cut(s)	94
Eco130I	CCWWGG	2 cut(s)	833, 922
Eco31I	GGTCTC	1 cut(s)	76
Eco47I	GGWCC	1 cut(s)	977
Eco57I	CTGAAG	1 cut(s)	1343
Eco81I	CCTNAGG	2 cut(s)	962, 1034
Eco88I	CYCGRG	1 cut(s)	207
Eco91I	GGTNACC	1 cut(s)	1564
EcoO109I	RGGNCCY	2 cut(s)	977, 1526
EcoO65I	GGTNACC	1 cut(s)	1564
EcoRI	GAATTC	1 cut(s)	1504
EcoRII	CCWGG	5 cut(s)	4, 573, 666, 980, 1798
EcoT14I	CCWWGG	2 cut(s)	833, 922
EcoT22I	ATGCAT	1 cut(s)	253
ErhI	CCWWGG	2 cut(s)	833, 922
FalI	AAGNNNNNCTT	6 cut(s)	810, 842, 1602, 1634, 1695, 1727
FaqI	GGGAC	1 cut(s)	990
FauI	CCCGC	1 cut(s)	947
FblI	GTMKAC	1 cut(s)	471
FokI	GGATG	4 cut(s)	224, 685, 694, 1315
FspAI	RTGCGCAY	1 cut(s)	719
FspBI	CTAG	5 cut(s)	42, 147, 522, 659, 1229
FspI	TGCGCA	1 cut(s)	719
GlaI	GCGC	1 cut(s)	719
GsaI	CCCAGC	1 cut(s)	611
GsuI	CTGGAG	1 cut(s)	394
HaeIII	GGCC	4 cut(s)	170, 626, 1527, 1771
HapII	CCGG	3 cut(s)	275, 914, 1752
HhaI	GCGC	1 cut(s)	720
Hin6I	GCGC	1 cut(s)	718
HinP1I	GCGC	1 cut(s)	718
HindIII	AAGCTT	1 cut(s)	881
HinfI	GANTC	9 cut(s)	395, 641, 1071, 1124, 1205, 1235, 1289, 1458, 1625
HpaII	CCGG	3 cut(s)	275, 914, 1752
HphI	GGTGA	6 cut(s)	532, 749, 1070, 1284, 1297, 1352
Hpy166II	GTNNAC	4 cut(s)	418, 472, 1012, 1360
Hpy188I	TCNGA	7 cut(s)	67, 90, 1054, 1070, 1135, 1186, 1438
Hpy8I	GTNNAC	4 cut(s)	418, 472, 1012, 1360
HpyAV	CCTTC	7 cut(s)	310, 400, 580, 944, 1168, 1308, 1550
HpyCH4III	ACNGT	6 cut(s)	422, 547, 555, 737, 1357, 1745
HpyCH4IV	ACGT	2 cut(s)	239, 1014
HpyF10VI	GCNNNNNNNGC	6 cut(s)	160, 212, 257, 1543, 1685, 1708
HpySE526I	ACGT	2 cut(s)	239, 1014
HspAI	GCGC	1 cut(s)	718
KflI	GGGWCCC	1 cut(s)	977
Kpn2I	TCCGGA	1 cut(s)	913
Kzo9I	GATC	4 cut(s)	95, 988, 1130, 1754
LmnI	GCTCC	3 cut(s)	90, 276, 1708
Lsp1109I	GCAGC	9 cut(s)	1065, 1130, 1177, 1198, 1236, 1430, 1436, 1530, 1558
LweI	GCATC	5 cut(s)	194, 202, 238, 1223, 1703
MaeI	CTAG	5 cut(s)	42, 147, 522, 659, 1229
MaeII	ACGT	2 cut(s)	239, 1014
MaeIII	GTNAC	4 cut(s)	538, 1058, 1564, 1784
MalI	GATC	4 cut(s)	97, 990, 1132, 1756
MboI	GATC	4 cut(s)	95, 988, 1130, 1754
MboII	GAAGA	8 cut(s)	41, 236, 443, 1202, 1266, 1336, 1622, 1678
MfeI	CAATTG	2 cut(s)	172, 195
MflI	RGATCY	1 cut(s)	95
MlsI	TGGCCA	2 cut(s)	170, 1771
MluCI	AATT	8 cut(s)	15, 172, 195, 281, 602, 1504, 1570, 1593
MluI	ACGCGT	1 cut(s)	161
MluNI	TGGCCA	2 cut(s)	170, 1771
MlyI	GAGTC	2 cut(s)	1133, 1214
MmeI	TCCRAC	2 cut(s)	997, 1048
Mox20I	TGGCCA	2 cut(s)	170, 1771
Mph1103I	ATGCAT	1 cut(s)	253
MroI	TCCGGA	1 cut(s)	913
MroXI	GAANNNNTTC	3 cut(s)	435, 555, 934
MscI	TGGCCA	2 cut(s)	170, 1771
MslI	CAYNNNNRTG	2 cut(s)	164, 545
Msp20I	TGGCCA	2 cut(s)	170, 1771
MspA1I	CMGCKG	1 cut(s)	1121
MspCI	CTTAAG	1 cut(s)	101
MspI	CCGG	3 cut(s)	275, 914, 1752
MspR9I	CCNGG	6 cut(s)	6, 275, 575, 668, 982, 1800
MunI	CAATTG	2 cut(s)	172, 195
MvaI	CCWGG	5 cut(s)	6, 575, 668, 982, 1800
MvnI	CGCG	2 cut(s)	163, 246
MwoI	GCNNNNNNNGC	6 cut(s)	160, 212, 257, 1543, 1685, 1708
NciI	CCSGG	1 cut(s)	275
NdeII	GATC	4 cut(s)	95, 988, 1130, 1754
NlaIV	GGNNCC	4 cut(s)	959, 978, 979, 1528
NmuCI	GTSAC	2 cut(s)	538, 1058
NruI	TCGCGA	1 cut(s)	246
NsbI	TGCGCA	1 cut(s)	719
NsiI	ATGCAT	1 cut(s)	253
NspI	RCATGY	6 cut(s)	615, 724, 857, 1343, 1431, 1618
NspV	TTCGAA	1 cut(s)	57
OliI	CACNNNNGTG	2 cut(s)	164, 545
PaeI	GCATGC	1 cut(s)	724
PciI	ACATGT	2 cut(s)	853, 1339
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PdmI	GAANNNNTTC	3 cut(s)	435, 555, 934
PfeI	GAWTC	7 cut(s)	395, 641, 1071, 1235, 1289, 1458, 1625
PflFI	GACNNNGTC	1 cut(s)	1064
PleI	GAGTC	2 cut(s)	1132, 1213
PpsI	GAGTC	2 cut(s)	1132, 1213
PpuMI	RGGWCCY	1 cut(s)	977
PscI	ACATGT	2 cut(s)	853, 1339
Psp5II	RGGWCCY	1 cut(s)	977
Psp6I	CCWGG	5 cut(s)	4, 573, 666, 980, 1798
PspEI	GGTNACC	1 cut(s)	1564
PspFI	CCCAGC	1 cut(s)	607
PspGI	CCWGG	5 cut(s)	4, 573, 666, 980, 1798
PspN4I	GGNNCC	4 cut(s)	959, 978, 979, 1528
PspPI	GGNCC	2 cut(s)	977, 1526
PspPPI	RGGWCCY	1 cut(s)	977
PstI	CTGCAG	4 cut(s)	1120, 1226, 1252, 1548
PstNI	CAGNNNCTG	1 cut(s)	1543
PsuI	RGATCY	1 cut(s)	95
PsyI	GACNNNGTC	1 cut(s)	1064
PvuII	CAGCTG	1 cut(s)	1121
RruI	TCGCGA	1 cut(s)	246
RsaI	GTAC	5 cut(s)	419, 1011, 1150, 1246, 1655
RsaNI	GTAC	5 cut(s)	418, 1010, 1149, 1245, 1654
RseI	CAYNNNNRTG	2 cut(s)	164, 545
Sau3AI	GATC	4 cut(s)	95, 988, 1130, 1754
Sau96I	GGNCC	2 cut(s)	977, 1526
ScaI	AGTACT	2 cut(s)	1150, 1246
SchI	GAGTC	2 cut(s)	1133, 1214
ScrFI	CCNGG	6 cut(s)	6, 275, 575, 668, 982, 1800
SfaNI	GCATC	5 cut(s)	194, 202, 238, 1223, 1703
SfcI	CTRYAG	5 cut(s)	1116, 1222, 1248, 1544, 1647
SfuI	TTCGAA	1 cut(s)	57
SinI	GGWCC	1 cut(s)	977
SmiMI	CAYNNNNRTG	2 cut(s)	164, 545
SmlI	CTYRAG	5 cut(s)	101, 127, 901, 1273, 1599
SmoI	CTYRAG	5 cut(s)	101, 127, 901, 1273, 1599
SphI	GCATGC	1 cut(s)	724
Sse9I	AATT	8 cut(s)	15, 172, 195, 281, 602, 1504, 1570, 1593
SsiI	CCGC	3 cut(s)	79, 623, 954
SspI	AATATT	1 cut(s)	1377
SspMI	CTAG	5 cut(s)	42, 147, 522, 659, 1229
StyD4I	CCNGG	6 cut(s)	4, 273, 573, 666, 980, 1798
StyI	CCWWGG	2 cut(s)	833, 922
TaaI	ACNGT	6 cut(s)	422, 547, 555, 737, 1357, 1745
TaiI	ACGT	2 cut(s)	242, 1017
TaqI	TCGA	3 cut(s)	57, 188, 285
TasI	AATT	8 cut(s)	15, 172, 195, 281, 602, 1504, 1570, 1593
TatI	WGTACW	3 cut(s)	417, 1148, 1244
TauI	GCSGC	1 cut(s)	626
TfiI	GAWTC	7 cut(s)	395, 641, 1071, 1235, 1289, 1458, 1625
TscAI	CASTG	2 cut(s)	427, 552
TseFI	GTSAC	2 cut(s)	538, 1058
TseI	GCWGC	9 cut(s)	1078, 1118, 1165, 1211, 1224, 1424, 1443, 1543, 1546
Tsp45I	GTSAC	2 cut(s)	538, 1058
TspDTI	ATGAA	5 cut(s)	444, 548, 689, 963, 1016
TspRI	CASTG	2 cut(s)	427, 552
Tth111I	GACNNNGTC	1 cut(s)	1064
Vha464I	CTTAAG	1 cut(s)	101
VpaK11BI	GGWCC	1 cut(s)	977
XapI	RAATTY	3 cut(s)	602, 1504, 1593
XbaI	TCTAGA	1 cut(s)	658
XceI	RCATGY	6 cut(s)	615, 724, 857, 1343, 1431, 1618
XmiI	GTMKAC	1 cut(s)	471
XmnI	GAANNNNTTC	3 cut(s)	435, 555, 934
XspI	CTAG	5 cut(s)	42, 147, 522, 659, 1229
ZrmI	AGTACT	2 cut(s)	1150, 1246
Zsp2I	ATGCAT	1 cut(s)	253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.