MD05G1180200.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018512
Physical Location & Seq
Forward (+)
30798145 .. 30798909
765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1180200.v1.1.491

Sequence Viewer

Length: 765 bp
ATGTCCACCGTGGAATTCCACGAAACTCTGATGAACCAAGTGCTTCCCTCGGCCTGGTCGCACAATCCTCTAACTACCCTCAAACTCATATGCAACTTCATAGACAAACGCGGCAACGGTGGAAAACGCAACCAAGACAAAGCTTTCTTCACAGCGGCGGTTTGGCTCTACCAGAACCACCCCAAAACTCTTGCCTGCGATCTCGTGACAATTTCTGGCTCCTTCGGGCATATCCTAGACCTTCCATGCATTCTTTCGCAGGTTCTACCGGGCGAAGCTCGCTTGAAGTTCACTAGGAGGATAAGGGAGGAGGCGGATTCAAAAATGGTGAAGGTAAAGGAAAAGGATGCCAAGTCAAAAGCAAGCGACTTCTTGTTGAGGAGGAAGACGATCCACAAGGCGGCTCAAAGAGATACGACCGCTTTTTGCACGATTGTGTTTCAGATATTTTCGCCCAATGCCTCAAGTCTGATATTGAAAAATTGCATAATCACAATGATCATGACCATGACCATCATGATGATTACTGCTTGGAGATTACCCAAGCGGCTACCTCTTTGCCTTGTTGATCATCATATGTTGTTCGAAAACATTGCAAGGAAGGTTTTCCCGCCAGAATCATGCCGCCGAGAAGGGGATGGTAGTGTTTGTTGTATTGACCGACTGAGGAAGGAGGTTTTGTCGCCCTTGAGAAAAACTATGGCGCCAAGGGGCTGGGAACCTGCCAATCGGTGGGGTTATAGAGCAGAGTACAAACGAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

255

Amino Acids

29.18

Weight (kDa)

9.75

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 9 - 129 1.5e-17 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 250, 730
AccB1I GGYRCC 1 cut(s) 703
AccB7I CCANNNNNTGG 1 cut(s) 732
AccII CGCG 1 cut(s) 111
AciI CCGC 9 cut(s) 111, 155, 158, 314, 401, 420, 547, 611, 625
AclWI GGATC 1 cut(s) 385
AcsI RAATTY 1 cut(s) 14
AcyI GRCGYC 1 cut(s) 704
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 4 cut(s) 54, 400, 634, 732
AgsI TTSAA 3 cut(s) 286, 321, 478
AjnI CCWGG 1 cut(s) 53
AleI CACNNNNGTG 1 cut(s) 434
AluBI AGCT 2 cut(s) 143, 278
AluI AGCT 2 cut(s) 143, 278
AlwI GGATC 1 cut(s) 385
AoxI GGCC 1 cut(s) 51
ApoI RAATTY 1 cut(s) 14
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 1 cut(s) 706
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 340
AsuII TTCGAA 1 cut(s) 585
BanI GGYRCC 1 cut(s) 703
BauI CACGAG 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 392
BccI CCATC 2 cut(s) 521, 632
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 1 cut(s) 55
BclI TGATCA 2 cut(s) 498, 568
BcnI CCSGG 1 cut(s) 270
BfaI CTAG 2 cut(s) 236, 294
BfoI RGCGCY 1 cut(s) 707
BfuAI ACCTGC 2 cut(s) 250, 730
BisI GCNGC 5 cut(s) 112, 156, 402, 548, 625
BlsI GCNGC 5 cut(s) 113, 157, 403, 549, 626
Bme1390I CCNGG 2 cut(s) 55, 270
BmiI GGNNCC 3 cut(s) 220, 705, 720
BmrFI CCNGG 2 cut(s) 55, 270
BmsI GCATC 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 392
Bpu14I TTCGAA 1 cut(s) 585
BpuEI CTTGAG 2 cut(s) 448, 709
BpuMI CCSGG 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 704
BsaJI CCNNGG 3 cut(s) 9, 48, 707
BsaXI ACNNNNNCTCC 2 cut(s) 665, 695
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 400, 634, 732
Bse3DI GCAATG 1 cut(s) 591
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 3 cut(s) 9, 48, 707
BseGI GGATG 2 cut(s) 352, 643
BseLI CCNNNNNNNGG 4 cut(s) 54, 400, 634, 732
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 1 cut(s) 656
BseRI GAGGAG 2 cut(s) 323, 394
BseYI CCCAGC 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 703
BsiEI CGRYCG 1 cut(s) 420
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 4 cut(s) 54, 400, 634, 732
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 1 cut(s) 53
Bsp119I TTCGAA 1 cut(s) 585
Bsp143I GATC 4 cut(s) 199, 390, 498, 568
BspACI CCGC 9 cut(s) 111, 155, 158, 314, 401, 420, 547, 611, 625
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 657
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 2 cut(s) 501, 516
BspLI GGNNCC 3 cut(s) 220, 705, 720
BspMI ACCTGC 2 cut(s) 250, 730
BspPI GGATC 1 cut(s) 385
BspT104I TTCGAA 1 cut(s) 585
BspT107I GGYRCC 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 591
BssECI CCNNGG 3 cut(s) 9, 48, 707
BssMI GATC 4 cut(s) 199, 390, 498, 568
BssNI GRCGYC 1 cut(s) 704
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 707
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 55
Bst4CI ACNGT 2 cut(s) 10, 119
BstACI GRCGYC 1 cut(s) 704
BstBI TTCGAA 1 cut(s) 585
BstC8I GCNNGC 3 cut(s) 196, 280, 364
BstDEI CTNAG 1 cut(s) 665
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 352, 643
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 707
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 4 cut(s) 202, 393, 501, 571
BstMBI GATC 4 cut(s) 199, 390, 498, 568
BstMCI CGRYCG 1 cut(s) 420
BstMWI GCNNNNNNNGC 1 cut(s) 279
BstNI CCWGG 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 53, 268
BstUI CGCG 1 cut(s) 111
BstV2I GAAGAC 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 714
BsuRI GGCC 1 cut(s) 53
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 2 cut(s) 352, 643
BveI ACCTGC 2 cut(s) 250, 730
Cac8I GCNNGC 3 cut(s) 196, 280, 364
CciI TCATGA 2 cut(s) 501, 516
CfoI GCGC 1 cut(s) 706
Csp6I GTAC 1 cut(s) 751
CviAII CATG 5 cut(s) 246, 502, 508, 517, 621
CviJI RGCY 8 cut(s) 53, 143, 166, 219, 278, 404, 550, 714
CviKI_1 RGCY 8 cut(s) 53, 143, 166, 219, 278, 404, 550, 714
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 1 cut(s) 665
DinI GGCGCC 1 cut(s) 705
DpnI GATC 4 cut(s) 201, 392, 500, 570
DpnII GATC 4 cut(s) 199, 390, 498, 568
EciI GGCGGA 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 707
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 707
EcoT22I ATGCAT 1 cut(s) 251
EgeI GGCGCC 1 cut(s) 705
EheI GGCGCC 1 cut(s) 705
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 5 cut(s) 249, 505, 511, 520, 624
FatI CATG 5 cut(s) 245, 501, 507, 516, 620
FauI CCCGC 1 cut(s) 618
FauNDI CATATG 2 cut(s) 89, 576
FbaI TGATCA 2 cut(s) 498, 568
Fnu4HI GCNGC 5 cut(s) 112, 156, 402, 548, 625
FokI GGATG 2 cut(s) 359, 650
Fsp4HI GCNGC 5 cut(s) 112, 156, 402, 548, 625
FspBI CTAG 2 cut(s) 236, 294
GlaI GCGC 1 cut(s) 705
GluI GCNGC 5 cut(s) 112, 156, 402, 548, 625
GsaI CCCAGC 1 cut(s) 718
HaeII RGCGCY 1 cut(s) 707
HaeIII GGCC 1 cut(s) 53
HapII CCGG 1 cut(s) 269
HhaI GCGC 1 cut(s) 706
Hin1I GRCGYC 1 cut(s) 704
Hin1II CATG 5 cut(s) 249, 505, 511, 520, 624
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 2 cut(s) 317, 617
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 2 cut(s) 6, 291
Hpy188I TCNGA 3 cut(s) 30, 444, 471
Hpy188III TCNNGA 3 cut(s) 205, 502, 517
Hpy8I GTNNAC 2 cut(s) 6, 291
HpyAV CCTTC 6 cut(s) 232, 251, 325, 595, 626, 664
HpyCH4III ACNGT 2 cut(s) 10, 119
HpyCH4V TGCA 5 cut(s) 93, 249, 429, 486, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 279
HpyF3I CTNAG 1 cut(s) 665
Hsp92I GRCGYC 1 cut(s) 704
Hsp92II CATG 5 cut(s) 249, 505, 511, 520, 624
HspAI GCGC 1 cut(s) 704
KasI GGCGCC 1 cut(s) 703
Ksp22I TGATCA 2 cut(s) 498, 568
Kzo9I GATC 4 cut(s) 199, 390, 498, 568
LmnI GCTCC 1 cut(s) 224
LweI GCATC 1 cut(s) 337
MaeI CTAG 2 cut(s) 236, 294
MaeIII GTNAC 1 cut(s) 205
MalI GATC 4 cut(s) 201, 392, 500, 570
MboI GATC 4 cut(s) 199, 390, 498, 568
MboII GAAGA 2 cut(s) 139, 397
MluCI AATT 3 cut(s) 14, 210, 481
Mly113I GGCGCC 1 cut(s) 704
Mph1103I ATGCAT 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 605
MslI CAYNNNNRTG 3 cut(s) 434, 506, 518
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 2 cut(s) 55, 270
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 1 cut(s) 55
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 279
NarI GGCGCC 1 cut(s) 704
NciI CCSGG 1 cut(s) 270
NdeI CATATG 2 cut(s) 89, 576
NdeII GATC 4 cut(s) 199, 390, 498, 568
NlaIII CATG 5 cut(s) 249, 505, 511, 520, 624
NlaIV GGNNCC 3 cut(s) 220, 705, 720
NmeAIII GCCGAG 2 cut(s) 29, 653
NmuCI GTSAC 1 cut(s) 205
NsiI ATGCAT 1 cut(s) 251
NspV TTCGAA 1 cut(s) 585
OliI CACNNNNGTG 1 cut(s) 434
PagI TCATGA 2 cut(s) 501, 516
PctI GAATGC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 317, 617
PflMI CCANNNNNTGG 1 cut(s) 732
PkrI GCNGC 5 cut(s) 113, 157, 403, 549, 626
PluTI GGCGCC 1 cut(s) 707
Psp6I CCWGG 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 3 cut(s) 220, 705, 720
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
RseI CAYNNNNRTG 3 cut(s) 434, 506, 518
SatI GCNGC 5 cut(s) 112, 156, 402, 548, 625
Sau3AI GATC 4 cut(s) 199, 390, 498, 568
ScrFI CCNGG 2 cut(s) 55, 270
SetI ASST 9 cut(s) 145, 243, 264, 280, 336, 556, 606, 678, 724
SfaNI GCATC 1 cut(s) 337
SfoI GGCGCC 1 cut(s) 705
SfuI TTCGAA 1 cut(s) 585
SmiMI CAYNNNNRTG 3 cut(s) 434, 506, 518
SmlI CTYRAG 2 cut(s) 463, 688
SmoI CTYRAG 2 cut(s) 463, 688
Sse9I AATT 3 cut(s) 14, 210, 481
SsiI CCGC 9 cut(s) 111, 155, 158, 314, 401, 420, 547, 611, 625
SspDI GGCGCC 1 cut(s) 703
SspMI CTAG 2 cut(s) 236, 294
StyD4I CCNGG 2 cut(s) 53, 268
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 2 cut(s) 10, 119
TaqI TCGA 1 cut(s) 585
TaqII GACCGA 1 cut(s) 675
TasI AATT 3 cut(s) 14, 210, 481
TatI WGTACW 1 cut(s) 750
TauI GCSGC 5 cut(s) 114, 158, 404, 550, 627
TfiI GAWTC 2 cut(s) 317, 617
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 47, 88
Van91I CCANNNNNTGG 1 cut(s) 732
XapI RAATTY 1 cut(s) 14
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 2 cut(s) 236, 294
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.