MD05G1257700.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018512
Physical Location & Seq
Reverse (-)
39401765 .. 39402214
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1257700.v1.1.491

Sequence Viewer

Length: 450 bp
ATGCTGTTCAAAAACATAGCAAGGAAGGTTTTCCCGCCAGAATCATGCCGCCGAGAAGGGGACGGTAGTGTTTGTTGTAATTACCGACTGAGGAAGGAGGTTTTGTCGCCCTTGAGAAAAACTAAGGCGCCAGAGGGCTGGGAACCTGCCAACCGGTGGGGTTATAGAGCGGAGTCCAAGCGAAGGCAACTAGCTGCAGCCAATGAGATTGATCCTGGAGATGCCGCGCTGCTTCCGCATGACATATTAAGCTATGTCATGTATCCCGAGCTCGAGCAAGATGCTGATGTTAAGTGGAAGAAAATGGTGAAGGTCTACTTAAAGCAGGGGAAGCTGAAGAATTGGTTGGCTGTGTGTGATGTCGACAACTCCTTGTCGGCGGGTTTGGCTTTGGGGCTTTTGTTGCCTCAATTGAGTGACGAGCCATGGAAGGGAAATGTGGTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

150

Amino Acids

16.88

Weight (kDa)

9.04

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 156
AccBSI CCGCTC 1 cut(s) 170
AccI GTMKAC 2 cut(s) 315, 363
AccII CGCG 1 cut(s) 227
AciI CCGC 6 cut(s) 35, 49, 170, 225, 236, 380
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 128
AfiI CCNNNNNNNGG 4 cut(s) 58, 156, 183, 431
AgeI ACCGGT 1 cut(s) 153
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 4 cut(s) 194, 252, 271, 334
AluI AGCT 4 cut(s) 194, 252, 271, 334
Alw21I GWGCWC 1 cut(s) 273
AlwI GGATC 1 cut(s) 206
Ama87I CYCGRG 2 cut(s) 266, 272
ApeKI GCWGC 3 cut(s) 194, 197, 229
AsiGI ACCGGT 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 2 cut(s) 130, 229
AsuHPI GGTGA 2 cut(s) 319, 436
AvaI CYCGRG 2 cut(s) 266, 272
BanI GGYRCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 181, 209, 216
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 216
BciVI GTATCC 1 cut(s) 273
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 154
BfuI GTATCC 1 cut(s) 273
BisI GCNGC 5 cut(s) 49, 195, 198, 225, 230
BlsI GCNGC 5 cut(s) 50, 196, 199, 226, 231
Bme1390I CCNGG 1 cut(s) 216
BmeT110I CYCGRG 2 cut(s) 266, 272
BmiI GGNNCC 2 cut(s) 129, 144
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 2 cut(s) 211, 271
BpmI CTGGAG 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 425
BsaWI WCCGGW 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 156, 183, 431
Bse118I RCCGGY 1 cut(s) 153
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 425
BseLI CCNNNNNNNGG 4 cut(s) 58, 156, 183, 431
BseMII CTCAG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 181, 209, 216
BseYI CCCAGC 1 cut(s) 138
Bsh1236I CGCG 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 127
BshTI ACCGGT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 2 cut(s) 266, 272
BsiSI CCGG 1 cut(s) 154
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 4 cut(s) 58, 156, 183, 431
BsmFI GGGAC 1 cut(s) 74
BsoBI CYCGRG 2 cut(s) 266, 272
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 211
Bsp19I CCATGG 1 cut(s) 425
BspACI CCGC 6 cut(s) 35, 49, 170, 225, 236, 380
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 2 cut(s) 129, 144
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 127
BsrBI CCGCTC 1 cut(s) 170
BsrFI RCCGGY 1 cut(s) 153
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 425
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 128
BstDEI CTNAG 2 cut(s) 89, 123
BstDSI CCRYGG 1 cut(s) 425
BstEII GGTNACC 1 cut(s) 442
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 130, 229
BstKTI GATC 1 cut(s) 214
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 4 cut(s) 235, 331, 386, 403
BstNI CCWGG 1 cut(s) 216
BstPI GGTNACC 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 214
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 3 cut(s) 181, 209, 216
BstXI CCANNNNNNTGG 1 cut(s) 138
BsuI GTATCC 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 425
BveI ACCTGC 1 cut(s) 154
CfoI GCGC 2 cut(s) 130, 229
Cfr10I RCCGGY 1 cut(s) 153
CspAI ACCGGT 1 cut(s) 153
CviAII CATG 4 cut(s) 45, 239, 259, 426
DdeI CTNAG 2 cut(s) 89, 123
DinI GGCGCC 1 cut(s) 129
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
Ecl136II GAGCTC 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 425
Eco24I GRGCYC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 2 cut(s) 266, 272
Eco91I GGTNACC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 271
EcoO65I GGTNACC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 273
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 425
FaeI CATG 4 cut(s) 48, 242, 262, 429
FaiI YATR 8 cut(s) 17, 46, 165, 240, 245, 255, 260, 427
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 74
FatI CATG 4 cut(s) 44, 238, 258, 425
FauI CCCGC 2 cut(s) 42, 373
FblI GTMKAC 2 cut(s) 315, 363
Fnu4HI GCNGC 5 cut(s) 49, 195, 198, 225, 230
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 5 cut(s) 49, 195, 198, 225, 230
FspBI CTAG 1 cut(s) 191
GlaI GCGC 2 cut(s) 129, 228
GluI GCNGC 5 cut(s) 49, 195, 198, 225, 230
GsaI CCCAGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 237
HaeII RGCGCY 1 cut(s) 131
HapII CCGG 1 cut(s) 154
HhaI GCGC 2 cut(s) 130, 229
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 4 cut(s) 48, 242, 262, 429
Hin6I GCGC 2 cut(s) 128, 227
HinP1I GCGC 2 cut(s) 128, 227
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 2 cut(s) 41, 173
HpaII CCGG 1 cut(s) 154
HphI GGTGA 2 cut(s) 319, 436
Hpy166II GTNNAC 2 cut(s) 316, 364
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 2 cut(s) 316, 364
HpyAV CCTTC 6 cut(s) 19, 50, 88, 177, 304, 424
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 331, 386, 403
HpyF3I CTNAG 2 cut(s) 89, 123
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 4 cut(s) 48, 242, 262, 429
HspAI GCGC 2 cut(s) 128, 227
KasI GGCGCC 1 cut(s) 127
Kzo9I GATC 1 cut(s) 211
LpnPI CCDG 8 cut(s) 51, 124, 144, 159, 167, 201, 228, 311
Lsp1109I GCAGC 3 cut(s) 181, 209, 216
LweI GCATC 2 cut(s) 211, 271
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 416, 442
MalI GATC 1 cut(s) 213
MbiI CCGCTC 1 cut(s) 170
MboI GATC 1 cut(s) 211
MboII GAAGA 2 cut(s) 310, 349
MfeI CAATTG 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 3 cut(s) 79, 340, 410
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 4 cut(s) 84, 91, 127, 417
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 3 cut(s) 248, 291, 320
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 216
MunI CAATTG 1 cut(s) 410
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 235, 331, 386, 403
NarI GGCGCC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 425
NdeII GATC 1 cut(s) 211
NlaIII CATG 4 cut(s) 48, 242, 262, 429
NlaIV GGNNCC 2 cut(s) 129, 144
NmeAIII GCCGAG 1 cut(s) 77
NmuCI GTSAC 2 cut(s) 416, 442
PaeR7I CTCGAG 1 cut(s) 272
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 156
PfoI TCCNGGA 1 cut(s) 214
PinAI ACCGGT 1 cut(s) 153
PkrI GCNGC 5 cut(s) 50, 196, 199, 226, 231
PleI GAGTC 1 cut(s) 181
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 214
PspEI GGTNACC 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 129, 144
PspXI VCTCGAGB 1 cut(s) 272
PstI CTGCAG 1 cut(s) 199
SacI GAGCTC 1 cut(s) 273
SalI GTCGAC 1 cut(s) 362
SaqAI TTAA 3 cut(s) 248, 291, 320
SatI GCNGC 5 cut(s) 49, 195, 198, 225, 230
Sau3AI GATC 1 cut(s) 211
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 273
SetI ASST 9 cut(s) 30, 102, 148, 196, 254, 273, 315, 336, 449
SfaNI GCATC 2 cut(s) 211, 271
SfcI CTRYAG 1 cut(s) 195
SfoI GGCGCC 1 cut(s) 129
Sfr274I CTCGAG 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 112, 272
SmoI CTYRAG 2 cut(s) 112, 272
Sse9I AATT 3 cut(s) 79, 340, 410
SsiI CCGC 6 cut(s) 35, 49, 170, 225, 236, 380
SspDI GGCGCC 1 cut(s) 127
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 273, 363
TasI AATT 3 cut(s) 79, 340, 410
TauI GCSGC 2 cut(s) 51, 227
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 3 cut(s) 248, 291, 320
Tru9I TTAA 3 cut(s) 248, 291, 320
TseFI GTSAC 2 cut(s) 416, 442
TseI GCWGC 3 cut(s) 194, 197, 229
Tsp45I GTSAC 2 cut(s) 416, 442
Van91I CCANNNNNTGG 1 cut(s) 156
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 2 cut(s) 315, 363
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.