MD05G1233100.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022428
Physical Location & Seq
Forward (+)
36591832 .. 36593123
1292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1233100.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGTGAAGAGAAAGACTAGTAGGGTTAGGGTTAGGGTTGAATCTGATAAGGATGAGGAAATTTTGGGGACTAATAAGCTTGAATTGAGAAAGTTGAAGAGGATTCGCGAAGAAGAGGAATCGCTCAAGATGTCCTACCGACGTCAAGAGGAGTTTGAACGGCAGGAACTAGAGAGACTGGCGTGTGTATCTTCTTCCTTTGCTGCCTGCCGCCTTGAAATTCAGGCAGCCGAGATATTTAGAAAACAGGTGGAAGCGCTGAAAAAGCGCGAGTTGGAAAAACGGTTGATATCCCTTGGCTACACATTCAACCCAGACGATGGAATATTACTTCATCACCACTTAAGGGAAAAGTGCCTCGAAAACGCTCTTGGTTTTTATGCCATCCTTGAAGAAGCTGACGTCTACGCAAAGCATCCTCAGAACCTGATAGAGAACTACCGTGCTCTCGCTGCTGGAAATGTTGGGTATTTCTTCACCCGTAGCCGCCCAAATCAAACTCAACCTGATACTAGTACAAGTACAGATCATGTTCAGGCCGGACATTGGATCTTTGGCAAAGAATTGGACGTGCTTCATCAGGGCGAGAAAGTCGGTGTTAAACAATTACTAGAGTATTGTGCAGCGGGCGCCAAGACCGGATACAAGATCATCGAGTACAGACTCGATCCTTGTCCAGACAACTTAAAGGATGGTCCTTGGTTCCTTTGTAAGCTGTACAAGGACGGGAGTTACATGGAGGTGCAGAGTTCGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

253

Amino Acids

29.25

Weight (kDa)

7.62

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 100 - 222 8.5e-15 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022428)

Species Orthologous Gene IDs
malus_domestica MD05G1233100.v1.1 MD05G1233300.v1.1 MD05G1233400.v1.1
pyrus_communis pycom05g21100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 145, 405
AccB1I GGYRCC 1 cut(s) 629
AccB7I CCANNNNNTGG 1 cut(s) 320
AccI GTMKAC 1 cut(s) 405
AccII CGCG 2 cut(s) 108, 270
AciI CCGC 3 cut(s) 211, 487, 626
AclWI GGATC 2 cut(s) 557, 662
AcsI RAATTY 2 cut(s) 60, 219
AcyI GRCGYC 3 cut(s) 142, 402, 630
AfaI GTAC 4 cut(s) 517, 523, 659, 719
AfeI AGCGCT 1 cut(s) 258
AfiI CCNNNNNNNGG 3 cut(s) 320, 346, 546
AflII CTTAAG 1 cut(s) 343
AgsI TTSAA 7 cut(s) 41, 83, 97, 158, 218, 310, 392
AhlI ACTAGT 2 cut(s) 17, 512
AjiI CACGTC 1 cut(s) 571
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 3 cut(s) 79, 398, 715
AluI AGCT 3 cut(s) 79, 398, 715
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 2 cut(s) 557, 662
AlwNI CAGNNNCTG 1 cut(s) 427
Aor51HI AGCGCT 1 cut(s) 258
AoxI GGCC 1 cut(s) 537
ApeKI GCWGC 4 cut(s) 203, 227, 452, 623
ApoI RAATTY 2 cut(s) 60, 219
AspLEI GCGC 3 cut(s) 259, 270, 632
AspS9I GGNCC 1 cut(s) 695
AsuHPI GGTGA 3 cut(s) 16, 329, 469
AvaII GGWCC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 629
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 4 cut(s) 190, 239, 439, 635
BccI CCATC 3 cut(s) 314, 392, 686
BceAI ACGGC 1 cut(s) 176
BciVI GTATCC 1 cut(s) 635
BcoDI GTCTC 1 cut(s) 169
BcuI ACTAGT 2 cut(s) 17, 512
BfaI CTAG 4 cut(s) 18, 170, 513, 611
BfoI RGCGCY 2 cut(s) 260, 633
BfrI CTTAAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 635
BisI GCNGC 6 cut(s) 204, 211, 228, 453, 487, 624
BlsI GCNGC 6 cut(s) 205, 212, 229, 454, 488, 625
Bme18I GGWCC 1 cut(s) 695
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 2 cut(s) 631, 704
BmsI GCATC 1 cut(s) 424
BpuEI CTTGAG 1 cut(s) 110
BsaHI GRCGYC 3 cut(s) 142, 402, 630
BsaJI CCNNGG 2 cut(s) 295, 698
BsaWI WCCGGW 1 cut(s) 638
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 346, 546
Bse1I ACTGG 1 cut(s) 183
BseDI CCNNGG 2 cut(s) 295, 698
BseGI GGATG 4 cut(s) 58, 384, 415, 697
BseLI CCNNNNNNNGG 3 cut(s) 320, 346, 546
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 4 cut(s) 190, 239, 439, 635
BsgI GTGCAG 1 cut(s) 642
Bsh1236I CGCG 2 cut(s) 108, 270
BshFI GGCC 1 cut(s) 539
BshNI GGYRCC 1 cut(s) 629
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 2 cut(s) 540, 639
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 3 cut(s) 320, 346, 546
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 82
BsnI GGCC 1 cut(s) 539
Bsp1286I GDGCHC 1 cut(s) 448
Bsp1407I TGTACA 1 cut(s) 717
Bsp143I GATC 4 cut(s) 526, 549, 648, 667
Bsp68I TCGCGA 1 cut(s) 108
BspACI CCGC 3 cut(s) 211, 487, 626
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 1 cut(s) 433
BspFNI CGCG 2 cut(s) 108, 270
BspLI GGNNCC 2 cut(s) 631, 704
BspPI GGATC 2 cut(s) 557, 662
BspT107I GGYRCC 1 cut(s) 629
BspTI CTTAAG 1 cut(s) 343
BsrGI TGTACA 1 cut(s) 717
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 295, 698
BssMI GATC 4 cut(s) 526, 549, 648, 667
BssNI GRCGYC 3 cut(s) 142, 402, 630
BssT1I CCWWGG 2 cut(s) 295, 698
Bst4CI ACNGT 2 cut(s) 285, 443
Bst6I CTCTTC 3 cut(s) 2, 92, 108
BstACI GRCGYC 3 cut(s) 142, 402, 630
BstAFI CTTAAG 1 cut(s) 343
BstAUI TGTACA 1 cut(s) 717
BstC8I GCNNGC 2 cut(s) 208, 628
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 4 cut(s) 58, 384, 415, 697
BstFNI CGCG 2 cut(s) 108, 270
BstH2I RGCGCY 2 cut(s) 260, 633
BstHHI GCGC 3 cut(s) 259, 270, 632
BstKTI GATC 4 cut(s) 529, 552, 651, 670
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 4 cut(s) 526, 549, 648, 667
BstMWI GCNNNNNNNGC 4 cut(s) 265, 452, 629, 751
BstUI CGCG 2 cut(s) 108, 270
BstV1I GCAGC 4 cut(s) 190, 239, 439, 635
BstX2I RGATCY 1 cut(s) 549
BstYI RGATCY 1 cut(s) 549
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 1 cut(s) 539
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 4 cut(s) 58, 384, 415, 697
BtuMI TCGCGA 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 208, 628
CaiI CAGNNNCTG 1 cut(s) 427
CfoI GCGC 3 cut(s) 259, 270, 632
Cfr13I GGNCC 1 cut(s) 695
Csp6I GTAC 4 cut(s) 516, 522, 658, 718
CviAII CATG 2 cut(s) 530, 736
CviJI RGCY 7 cut(s) 79, 230, 300, 398, 486, 539, 715
CviKI_1 RGCY 7 cut(s) 79, 230, 300, 398, 486, 539, 715
CviQI GTAC 4 cut(s) 516, 522, 658, 718
DdeI CTNAG 1 cut(s) 420
DinI GGCGCC 1 cut(s) 631
DpnI GATC 4 cut(s) 528, 551, 650, 669
DpnII GATC 4 cut(s) 526, 549, 648, 667
Eam1104I CTCTTC 3 cut(s) 2, 92, 108
EarI CTCTTC 3 cut(s) 2, 92, 108
Eco130I CCWWGG 2 cut(s) 295, 698
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 695
Eco47III AGCGCT 1 cut(s) 258
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 2 cut(s) 295, 698
EgeI GGCGCC 1 cut(s) 631
EheI GGCGCC 1 cut(s) 631
ErhI CCWWGG 2 cut(s) 295, 698
FaeI CATG 2 cut(s) 533, 739
FaiI YATR 3 cut(s) 381, 531, 737
FaqI GGGAC 1 cut(s) 82
FatI CATG 2 cut(s) 529, 735
FauI CCCGC 1 cut(s) 619
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 6 cut(s) 204, 211, 228, 453, 487, 624
FokI GGATG 4 cut(s) 65, 371, 402, 704
Fsp4HI GCNGC 6 cut(s) 204, 211, 228, 453, 487, 624
FspBI CTAG 4 cut(s) 18, 170, 513, 611
GlaI GCGC 3 cut(s) 258, 269, 631
GluI GCNGC 6 cut(s) 204, 211, 228, 453, 487, 624
HaeII RGCGCY 2 cut(s) 260, 633
HaeIII GGCC 1 cut(s) 539
HapII CCGG 2 cut(s) 540, 639
HhaI GCGC 3 cut(s) 259, 270, 632
Hin1I GRCGYC 3 cut(s) 142, 402, 630
Hin1II CATG 2 cut(s) 533, 739
Hin6I GCGC 3 cut(s) 257, 268, 630
HinP1I GCGC 3 cut(s) 257, 268, 630
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 4 cut(s) 41, 103, 119, 663
HpaII CCGG 2 cut(s) 540, 639
HphI GGTGA 3 cut(s) 16, 329, 469
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 2 cut(s) 46, 423
Hpy188III TCNNGA 4 cut(s) 107, 127, 146, 677
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 1 cut(s) 144
HpyCH4III ACNGT 2 cut(s) 285, 443
HpyCH4IV ACGT 3 cut(s) 142, 402, 570
HpyCH4V TGCA 2 cut(s) 623, 745
HpyF10VI GCNNNNNNNGC 4 cut(s) 265, 452, 629, 751
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 3 cut(s) 142, 402, 570
Hsp92I GRCGYC 3 cut(s) 142, 402, 630
Hsp92II CATG 2 cut(s) 533, 739
HspAI GCGC 3 cut(s) 257, 268, 630
KasI GGCGCC 1 cut(s) 629
Kzo9I GATC 4 cut(s) 526, 549, 648, 667
Lsp1109I GCAGC 4 cut(s) 190, 239, 439, 635
LweI GCATC 1 cut(s) 424
MaeI CTAG 4 cut(s) 18, 170, 513, 611
MaeII ACGT 3 cut(s) 142, 402, 570
MaeIII GTNAC 1 cut(s) 731
MalI GATC 4 cut(s) 528, 551, 650, 669
MboI GATC 4 cut(s) 526, 549, 648, 667
MboII GAAGA 8 cut(s) 19, 109, 122, 125, 183, 186, 404, 466
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 5 cut(s) 60, 83, 219, 563, 605
Mly113I GGCGCC 1 cut(s) 630
MlyI GAGTC 1 cut(s) 657
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 7 cut(s) 49, 93, 109, 142, 368, 429, 733
MseI TTAA 3 cut(s) 344, 600, 686
MslI CAYNNNNRTG 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 343
MspI CCGG 2 cut(s) 540, 639
MvnI CGCG 2 cut(s) 108, 270
MwoI GCNNNNNNNGC 4 cut(s) 265, 452, 629, 751
NarI GGCGCC 1 cut(s) 630
NdeII GATC 4 cut(s) 526, 549, 648, 667
NlaIII CATG 2 cut(s) 533, 739
NlaIV GGNNCC 2 cut(s) 631, 704
NmeAIII GCCGAG 1 cut(s) 256
NruI TCGCGA 1 cut(s) 108
PfeI GAWTC 3 cut(s) 41, 103, 119
PflMI CCANNNNNTGG 1 cut(s) 320
PkrI GCNGC 6 cut(s) 205, 212, 229, 454, 488, 625
PleI GAGTC 1 cut(s) 657
PluTI GGCGCC 1 cut(s) 633
PpsI GAGTC 1 cut(s) 657
PspN4I GGNNCC 2 cut(s) 631, 704
PspPI GGNCC 1 cut(s) 695
PstNI CAGNNNCTG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 549
RruI TCGCGA 1 cut(s) 108
RsaI GTAC 4 cut(s) 517, 523, 659, 719
RsaNI GTAC 4 cut(s) 516, 522, 658, 718
RseI CAYNNNNRTG 1 cut(s) 740
SaqAI TTAA 3 cut(s) 344, 600, 686
SatI GCNGC 6 cut(s) 204, 211, 228, 453, 487, 624
Sau3AI GATC 4 cut(s) 526, 549, 648, 667
Sau96I GGNCC 1 cut(s) 695
SchI GAGTC 1 cut(s) 657
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 1 cut(s) 424
SfoI GGCGCC 1 cut(s) 631
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 2 cut(s) 125, 343
SmoI CTYRAG 2 cut(s) 125, 343
SpeI ACTAGT 2 cut(s) 17, 512
Sse9I AATT 5 cut(s) 60, 83, 219, 563, 605
SsiI CCGC 3 cut(s) 211, 487, 626
SspDI GGCGCC 1 cut(s) 629
SspI AATATT 1 cut(s) 327
SspMI CTAG 4 cut(s) 18, 170, 513, 611
StyI CCWWGG 2 cut(s) 295, 698
TaaI ACNGT 2 cut(s) 285, 443
TaiI ACGT 3 cut(s) 145, 405, 573
TaqI TCGA 3 cut(s) 360, 654, 666
TasI AATT 5 cut(s) 60, 83, 219, 563, 605
TatI WGTACW 4 cut(s) 515, 521, 657, 717
TauI GCSGC 2 cut(s) 213, 489
TfiI GAWTC 3 cut(s) 41, 103, 119
Tru1I TTAA 3 cut(s) 344, 600, 686
Tru9I TTAA 3 cut(s) 344, 600, 686
TseI GCWGC 4 cut(s) 203, 227, 452, 623
TspDTI ATGAA 2 cut(s) 323, 566
Van91I CCANNNNNTGG 1 cut(s) 320
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 2 cut(s) 60, 219
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 4 cut(s) 18, 170, 513, 611
ZraI GACGTC 2 cut(s) 143, 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.