pycom05g21100
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0022428
Physical Location & Seq
Reverse (-)
23790734 .. 23792194
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21100.2

Sequence Viewer

Length: 819 bp
ATGAAAATAACGCGGGTCACTCAGATTTATCCGTCCCAAACGGACAACTTGGGGTTGGATTTTGGCTCAGAAGAATCAACAACGATAGCGATGGTGAAGAGAAAGACTAGTACTAATAATACTAGTAGAGTTAGGGTTAGGGTTGAATCTGACAAGGATGAGGAAATTTTGGGGACTAATAAGCTTGAATTGAGAAAGTTAAAGAGGATTCGCGAACAAGAGGAAGCGCTCAAGATTTCCCACCGACGTCAAGAGGATCTTGAACGGCAGGAACTAGAGAGACTGGCGTGGGTATCTTCTTCCTTTGCTGCCTGCCGCCTTGAAATTCAGGCAGCCGAGATGTTTAGAAAACAGGTGGAAGCACTGAAAAAGCGAGAGTTGGAAAAACGGTTGATATCCCTTGGTTACACATTCAACCCAGACGATGGAATATTACTTCATCACCACTTAAGGGAAAAGTGCCTCGAAAACGCTCTTGGTTTTTATGCCATTCTTGAAGAAGCTGACGTCTACGCAAAGCATCCTCAGAACCTAATAGAAAACTACCGTGCTCTTGCTGCTGGAAATGTTAGGTATTTCTTCACCCGTAGCCGCCCAAATGAAACTCAACCTGATACTAGTACAGGTACAGATCATGTTCAGGCCGGACATTGGATCTTTGGCAAAGAATTGGACGTGCTTCATCAGGGCGAGAAAGTCGGTGTTAAACAATTACTTGAGTATTGTGCAGCGGGCGCCAAGACCAGATATAAGATCGATCGAGTACAGACTCGATCCTTGTCCAGACAACTTAAAGGATGGTCCTTGGTTCCTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

273

Amino Acids

31.46

Weight (kDa)

9.12

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022428)

Species Orthologous Gene IDs
malus_domestica MD05G1233100.v1.1 MD05G1233300.v1.1 MD05G1233400.v1.1
pyrus_communis pycom05g21100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 250, 510
AccB1I GGYRCC 1 cut(s) 734
AccB7I CCANNNNNTGG 1 cut(s) 425
AccI GTMKAC 1 cut(s) 510
AccII CGCG 2 cut(s) 13, 213
AciI CCGC 4 cut(s) 13, 316, 592, 731
AclWI GGATC 3 cut(s) 264, 662, 768
AcsI RAATTY 2 cut(s) 165, 324
AcyI GRCGYC 3 cut(s) 247, 507, 735
AfaI GTAC 4 cut(s) 112, 622, 628, 765
AfeI AGCGCT 1 cut(s) 228
AfiI CCNNNNNNNGG 3 cut(s) 425, 451, 651
AflII CTTAAG 1 cut(s) 448
AgsI TTSAA 6 cut(s) 146, 188, 263, 323, 415, 497
AhlI ACTAGT 3 cut(s) 107, 122, 617
AjiI CACGTC 1 cut(s) 676
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AluBI AGCT 2 cut(s) 184, 503
AluI AGCT 2 cut(s) 184, 503
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 3 cut(s) 264, 662, 768
Aor51HI AGCGCT 1 cut(s) 228
AoxI GGCC 1 cut(s) 642
ApeKI GCWGC 4 cut(s) 308, 332, 557, 728
ApoI RAATTY 2 cut(s) 165, 324
AspLEI GCGC 2 cut(s) 229, 737
AspS9I GGNCC 1 cut(s) 801
AsuHPI GGTGA 3 cut(s) 106, 434, 574
AvaII GGWCC 1 cut(s) 801
BanI GGYRCC 1 cut(s) 734
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 4 cut(s) 295, 344, 544, 740
BccI CCATC 3 cut(s) 85, 419, 792
BceAI ACGGC 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 274
BcuI ACTAGT 3 cut(s) 107, 122, 617
BfaI CTAG 4 cut(s) 108, 123, 275, 618
BfoI RGCGCY 2 cut(s) 230, 738
BfrI CTTAAG 1 cut(s) 448
BisI GCNGC 6 cut(s) 309, 316, 333, 558, 592, 729
BlsI GCNGC 6 cut(s) 310, 317, 334, 559, 593, 730
BmcAI AGTACT 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 801
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 1 cut(s) 801
BmiI GGNNCC 2 cut(s) 736, 810
BmsI GCATC 1 cut(s) 529
BpuEI CTTGAG 2 cut(s) 215, 737
Bsa29I ATCGAT 1 cut(s) 756
BsaHI GRCGYC 3 cut(s) 247, 507, 735
BsaJI CCNNGG 2 cut(s) 400, 804
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 451, 651
Bse1I ACTGG 1 cut(s) 288
BseCI ATCGAT 1 cut(s) 756
BseDI CCNNGG 2 cut(s) 400, 804
BseGI GGATG 3 cut(s) 163, 520, 803
BseLI CCNNNNNNNGG 3 cut(s) 425, 451, 651
BseMII CTCAG 3 cut(s) 35, 81, 539
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 4 cut(s) 295, 344, 544, 740
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 2 cut(s) 13, 213
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 1 cut(s) 644
BshNI GGYRCC 1 cut(s) 734
BshVI ATCGAT 1 cut(s) 756
BsiEI CGRYCG 1 cut(s) 760
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 1 cut(s) 645
BslFI GGGAC 2 cut(s) 19, 187
BslI CCNNNNNNNGG 3 cut(s) 425, 451, 651
BsmAI GTCTC 1 cut(s) 274
BsmFI GGGAC 2 cut(s) 19, 187
BsnI GGCC 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 553
Bsp143I GATC 6 cut(s) 256, 631, 654, 753, 757, 773
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 4 cut(s) 13, 316, 592, 731
BspANI GGCC 1 cut(s) 644
BspCNI CTCAG 3 cut(s) 34, 80, 538
BspDI ATCGAT 1 cut(s) 756
BspFNI CGCG 2 cut(s) 13, 213
BspLI GGNNCC 2 cut(s) 736, 810
BspPI GGATC 3 cut(s) 264, 662, 768
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 1 cut(s) 448
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 2 cut(s) 400, 804
BssMI GATC 6 cut(s) 256, 631, 654, 753, 757, 773
BssNI GRCGYC 3 cut(s) 247, 507, 735
BssT1I CCWWGG 2 cut(s) 400, 804
Bst4CI ACNGT 2 cut(s) 390, 548
Bst6I CTCTTC 1 cut(s) 92
BstACI GRCGYC 3 cut(s) 247, 507, 735
BstAFI CTTAAG 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 313, 733
BstDEI CTNAG 3 cut(s) 21, 67, 525
BstF5I GGATG 3 cut(s) 163, 520, 803
BstFNI CGCG 2 cut(s) 13, 213
BstH2I RGCGCY 2 cut(s) 230, 738
BstHHI GCGC 2 cut(s) 229, 737
BstKTI GATC 6 cut(s) 259, 634, 657, 756, 760, 776
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 6 cut(s) 256, 631, 654, 753, 757, 773
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 2 cut(s) 557, 734
BstUI CGCG 2 cut(s) 13, 213
BstV1I GCAGC 4 cut(s) 295, 344, 544, 740
BstX2I RGATCY 2 cut(s) 256, 654
BstYI RGATCY 2 cut(s) 256, 654
Bsu15I ATCGAT 1 cut(s) 756
BsuRI GGCC 1 cut(s) 644
BsuTUI ATCGAT 1 cut(s) 756
BtgZI GCGATG 1 cut(s) 104
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 3 cut(s) 163, 520, 803
BtsIMutI CAGTG 1 cut(s) 362
BtuMI TCGCGA 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 313, 733
CfoI GCGC 2 cut(s) 229, 737
Cfr13I GGNCC 1 cut(s) 801
ClaI ATCGAT 1 cut(s) 756
Csp6I GTAC 4 cut(s) 111, 621, 627, 764
CviAII CATG 1 cut(s) 635
CviJI RGCY 6 cut(s) 66, 184, 335, 503, 591, 644
CviKI_1 RGCY 6 cut(s) 66, 184, 335, 503, 591, 644
CviQI GTAC 4 cut(s) 111, 621, 627, 764
DdeI CTNAG 3 cut(s) 21, 67, 525
DinI GGCGCC 1 cut(s) 736
DpnI GATC 6 cut(s) 258, 633, 656, 755, 759, 775
DpnII GATC 6 cut(s) 256, 631, 654, 753, 757, 773
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 2 cut(s) 400, 804
Eco32I GATATC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 801
Eco47III AGCGCT 1 cut(s) 228
EcoRV GATATC 1 cut(s) 396
EcoT14I CCWWGG 2 cut(s) 400, 804
EgeI GGCGCC 1 cut(s) 736
EheI GGCGCC 1 cut(s) 736
ErhI CCWWGG 2 cut(s) 400, 804
FaeI CATG 1 cut(s) 638
FaiI YATR 4 cut(s) 486, 636, 750, 815
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 2 cut(s) 19, 187
FatI CATG 1 cut(s) 634
FauI CCCGC 2 cut(s) 6, 724
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 6 cut(s) 309, 316, 333, 558, 592, 729
FokI GGATG 3 cut(s) 170, 507, 810
Fsp4HI GCNGC 6 cut(s) 309, 316, 333, 558, 592, 729
FspBI CTAG 4 cut(s) 108, 123, 275, 618
GlaI GCGC 2 cut(s) 228, 736
GluI GCNGC 6 cut(s) 309, 316, 333, 558, 592, 729
HaeII RGCGCY 2 cut(s) 230, 738
HaeIII GGCC 1 cut(s) 644
HapII CCGG 1 cut(s) 645
HhaI GCGC 2 cut(s) 229, 737
Hin1I GRCGYC 3 cut(s) 247, 507, 735
Hin1II CATG 1 cut(s) 638
Hin6I GCGC 2 cut(s) 227, 735
HinP1I GCGC 2 cut(s) 227, 735
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 4 cut(s) 74, 146, 208, 769
HpaII CCGG 1 cut(s) 645
HphI GGTGA 3 cut(s) 106, 434, 574
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 4 cut(s) 24, 70, 151, 528
Hpy188III TCNNGA 6 cut(s) 212, 232, 251, 260, 494, 783
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 1 cut(s) 249
HpyCH4III ACNGT 2 cut(s) 390, 548
HpyCH4IV ACGT 3 cut(s) 247, 507, 675
HpyCH4V TGCA 1 cut(s) 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 734
HpyF3I CTNAG 3 cut(s) 21, 67, 525
HpySE526I ACGT 3 cut(s) 247, 507, 675
Hsp92I GRCGYC 3 cut(s) 247, 507, 735
Hsp92II CATG 1 cut(s) 638
HspAI GCGC 2 cut(s) 227, 735
KasI GGCGCC 1 cut(s) 734
Kzo9I GATC 6 cut(s) 256, 631, 654, 753, 757, 773
Lsp1109I GCAGC 4 cut(s) 295, 344, 544, 740
LweI GCATC 1 cut(s) 529
MaeI CTAG 4 cut(s) 108, 123, 275, 618
MaeII ACGT 3 cut(s) 247, 507, 675
MaeIII GTNAC 2 cut(s) 16, 404
MalI GATC 6 cut(s) 258, 633, 656, 755, 759, 775
MboI GATC 6 cut(s) 256, 631, 654, 753, 757, 773
MboII GAAGA 6 cut(s) 83, 109, 288, 291, 509, 571
MflI RGATCY 2 cut(s) 256, 654
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 5 cut(s) 165, 188, 324, 668, 710
Mly113I GGCGCC 1 cut(s) 735
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 2 cut(s) 36, 360
MnlI CCTC 6 cut(s) 154, 198, 214, 247, 473, 534
MseI TTAA 4 cut(s) 200, 449, 705, 792
MspA1I CMGCKG 1 cut(s) 731
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 1 cut(s) 645
MvnI CGCG 2 cut(s) 13, 213
MwoI GCNNNNNNNGC 2 cut(s) 557, 734
NarI GGCGCC 1 cut(s) 735
NdeII GATC 6 cut(s) 256, 631, 654, 753, 757, 773
NlaIII CATG 1 cut(s) 638
NlaIV GGNNCC 2 cut(s) 736, 810
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 16
NruI TCGCGA 1 cut(s) 213
PfeI GAWTC 3 cut(s) 74, 146, 208
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 6 cut(s) 310, 317, 334, 559, 593, 730
Ple19I CGATCG 1 cut(s) 760
PleI GAGTC 1 cut(s) 763
PluTI GGCGCC 1 cut(s) 738
PpsI GAGTC 1 cut(s) 763
PspN4I GGNNCC 2 cut(s) 736, 810
PspPI GGNCC 1 cut(s) 801
PsuI RGATCY 2 cut(s) 256, 654
PvuI CGATCG 1 cut(s) 760
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 4 cut(s) 112, 622, 628, 765
RsaNI GTAC 4 cut(s) 111, 621, 627, 764
SaqAI TTAA 4 cut(s) 200, 449, 705, 792
SatI GCNGC 6 cut(s) 309, 316, 333, 558, 592, 729
Sau3AI GATC 6 cut(s) 256, 631, 654, 753, 757, 773
Sau96I GGNCC 1 cut(s) 801
ScaI AGTACT 1 cut(s) 112
SchI GAGTC 1 cut(s) 763
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 1 cut(s) 529
SfoI GGCGCC 1 cut(s) 736
SinI GGWCC 1 cut(s) 801
SmlI CTYRAG 3 cut(s) 230, 448, 716
SmoI CTYRAG 3 cut(s) 230, 448, 716
SpeI ACTAGT 3 cut(s) 107, 122, 617
Sse9I AATT 5 cut(s) 165, 188, 324, 668, 710
SsiI CCGC 4 cut(s) 13, 316, 592, 731
SspDI GGCGCC 1 cut(s) 734
SspI AATATT 1 cut(s) 432
SspMI CTAG 4 cut(s) 108, 123, 275, 618
StyI CCWWGG 2 cut(s) 400, 804
TaaI ACNGT 2 cut(s) 390, 548
TaiI ACGT 3 cut(s) 250, 510, 678
TaqI TCGA 4 cut(s) 465, 756, 760, 772
TasI AATT 5 cut(s) 165, 188, 324, 668, 710
TatI WGTACW 3 cut(s) 110, 620, 763
TauI GCSGC 2 cut(s) 318, 594
TfiI GAWTC 3 cut(s) 74, 146, 208
Tru1I TTAA 4 cut(s) 200, 449, 705, 792
Tru9I TTAA 4 cut(s) 200, 449, 705, 792
TscAI CASTG 1 cut(s) 369
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 4 cut(s) 308, 332, 557, 728
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 4 cut(s) 17, 428, 615, 671
TspGWI ACGGA 2 cut(s) 21, 56
TspRI CASTG 1 cut(s) 369
Van91I CCANNNNNTGG 1 cut(s) 425
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 801
XapI RAATTY 2 cut(s) 165, 324
XmiI GTMKAC 1 cut(s) 510
XspI CTAG 4 cut(s) 108, 123, 275, 618
ZraI GACGTC 2 cut(s) 248, 508
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.