MD05G1233300.v1.1

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022428
Physical Location & Seq
Reverse (-)
36608511 .. 36609977
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1233300.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGTGAAGAGAAAGACTAGTACTAATAATACTAGTAATACTAGCAGGGTTAGGGTTAGGGTTAGGGTTGAATCTGATAAGGATGAGGAAATTTTGGGGACTAATAAGCTTGAATTGAGAAAGTTGAAGAGGATTCGCGAAGAAGAGGAATCGCTCAAGATTTCCTTCCGACTTGCAGAGGAGCTTGAACGGCAGGAACTAGAGAGACTGGCGTGTGTATCTTCTTCCTTTGCTGCCTGCCGCCTTGAAATTCGGGCAGCCGAGATATTTAGAAAACAGGCGGAAGCACTGAAAAAGCGAGAGTTGGAAAAACGGTTGATATCCCTTGGTTACACATTCAACCCAGACGATGGAATATTACTTCATCACCACTTAAGGGAAAAGTGCCTCGAAAACGCTCTTGGTTTTTATGCCATCCTTGAAGAAGCTGACATCTACGCAAAGCATCCTCAGAACCTAATAGAAAACTACCGTGCTCTCGCTGCTGGAAATGTTGGGTATTTCTTCACCCGTAGCCGCCCAAATGAAACTCAACCTCATACTAGTACAAGTACAGATCATGTTCAGGCCGGACATTGGATCTTTGGCAAAGAATTGGACGTGCTTCATCAGGGCGAGAAAGTTGGTGTTAAACAATTACTAGAGTATTGTGCAGCGGGCGCCAAGACCGGATACAAGATCATCGAGTACAGACTCGATCCTTGTCCAGACAACTTAAAGGATGGTCCTTGGTTCCTTTGTAAGCTGTACAAGGACGGGAGTTACATGGAGGTGCAGAGTTCGCTCTAA

Protein Analysis

263

Amino Acids

30.19

Weight (kDa)

8.13

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 110 - 232 7.5e-15 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022428)

Species Orthologous Gene IDs
malus_domestica MD05G1233100.v1.1 MD05G1233300.v1.1 MD05G1233400.v1.1
pyrus_communis pycom05g21100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 350
AccII CGCG 1 cut(s) 138
AciI CCGC 4 cut(s) 241, 281, 517, 656
AclWI GGATC 2 cut(s) 587, 692
AcsI RAATTY 2 cut(s) 90, 249
AcyI GRCGYC 1 cut(s) 660
AfaI GTAC 5 cut(s) 22, 547, 553, 689, 749
AfiI CCNNNNNNNGG 3 cut(s) 350, 376, 576
AflII CTTAAG 1 cut(s) 373
AgsI TTSAA 7 cut(s) 71, 113, 127, 188, 248, 340, 422
AhlI ACTAGT 3 cut(s) 17, 32, 542
AjiI CACGTC 1 cut(s) 601
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 4 cut(s) 109, 184, 428, 745
AluI AGCT 4 cut(s) 109, 184, 428, 745
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 1 cut(s) 199
AlwI GGATC 2 cut(s) 587, 692
AoxI GGCC 1 cut(s) 567
ApeKI GCWGC 4 cut(s) 233, 257, 482, 653
ApoI RAATTY 2 cut(s) 90, 249
AspLEI GCGC 1 cut(s) 662
AspS9I GGNCC 1 cut(s) 725
AsuHPI GGTGA 3 cut(s) 16, 359, 499
AvaII GGWCC 1 cut(s) 725
BanI GGYRCC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 4 cut(s) 220, 269, 469, 665
BccI CCATC 3 cut(s) 344, 422, 716
BceAI ACGGC 1 cut(s) 206
BciVI GTATCC 1 cut(s) 665
BcoDI GTCTC 1 cut(s) 199
BcuI ACTAGT 3 cut(s) 17, 32, 542
BfaI CTAG 6 cut(s) 18, 33, 42, 200, 543, 641
BfoI RGCGCY 1 cut(s) 663
BfrI CTTAAG 1 cut(s) 373
BfuI GTATCC 1 cut(s) 665
BisI GCNGC 6 cut(s) 234, 241, 258, 483, 517, 654
BlsI GCNGC 6 cut(s) 235, 242, 259, 484, 518, 655
BmcAI AGTACT 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 725
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 725
BmiI GGNNCC 2 cut(s) 661, 734
BmsI GCATC 1 cut(s) 454
BpuEI CTTGAG 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 325, 728
BsaWI WCCGGW 1 cut(s) 668
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 376, 576
Bse1I ACTGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 325, 728
BseGI GGATG 4 cut(s) 88, 414, 445, 727
BseLI CCNNNNNNNGG 3 cut(s) 350, 376, 576
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 4 cut(s) 220, 269, 469, 665
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 569
BshNI GGYRCC 1 cut(s) 659
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 2 cut(s) 570, 669
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 350, 376, 576
BsmAI GTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 478
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 4 cut(s) 556, 579, 678, 697
Bsp68I TCGCGA 1 cut(s) 138
BspACI CCGC 4 cut(s) 241, 281, 517, 656
BspANI GGCC 1 cut(s) 569
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 661, 734
BspPI GGATC 2 cut(s) 587, 692
BspT107I GGYRCC 1 cut(s) 659
BspTI CTTAAG 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 1 cut(s) 213
BssECI CCNNGG 2 cut(s) 325, 728
BssMI GATC 4 cut(s) 556, 579, 678, 697
BssNI GRCGYC 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 325, 728
Bst4CI ACNGT 2 cut(s) 315, 473
Bst6I CTCTTC 3 cut(s) 2, 122, 138
BstACI GRCGYC 1 cut(s) 660
BstAFI CTTAAG 1 cut(s) 373
BstAUI TGTACA 1 cut(s) 747
BstC8I GCNNGC 2 cut(s) 238, 658
BstDEI CTNAG 1 cut(s) 450
BstF5I GGATG 4 cut(s) 88, 414, 445, 727
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 1 cut(s) 662
BstKTI GATC 4 cut(s) 559, 582, 681, 700
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 4 cut(s) 556, 579, 678, 697
BstMWI GCNNNNNNNGC 4 cut(s) 190, 482, 659, 781
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 4 cut(s) 220, 269, 469, 665
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BsuI GTATCC 1 cut(s) 665
BsuRI GGCC 1 cut(s) 569
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 4 cut(s) 88, 414, 445, 727
BtsIMutI CAGTG 1 cut(s) 287
BtuMI TCGCGA 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 238, 658
CfoI GCGC 1 cut(s) 662
Cfr13I GGNCC 1 cut(s) 725
Csp6I GTAC 5 cut(s) 21, 546, 552, 688, 748
CviAII CATG 2 cut(s) 560, 766
CviJI RGCY 7 cut(s) 109, 184, 260, 428, 516, 569, 745
CviKI_1 RGCY 7 cut(s) 109, 184, 260, 428, 516, 569, 745
CviQI GTAC 5 cut(s) 21, 546, 552, 688, 748
DdeI CTNAG 1 cut(s) 450
DinI GGCGCC 1 cut(s) 661
DpnI GATC 4 cut(s) 558, 581, 680, 699
DpnII GATC 4 cut(s) 556, 579, 678, 697
Eam1104I CTCTTC 3 cut(s) 2, 122, 138
EarI CTCTTC 3 cut(s) 2, 122, 138
EciI GGCGGA 1 cut(s) 296
Eco130I CCWWGG 2 cut(s) 325, 728
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 725
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 325, 728
EgeI GGCGCC 1 cut(s) 661
EheI GGCGCC 1 cut(s) 661
ErhI CCWWGG 2 cut(s) 325, 728
FaeI CATG 2 cut(s) 563, 769
FaiI YATR 4 cut(s) 411, 540, 561, 767
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 1 cut(s) 112
FatI CATG 2 cut(s) 559, 765
FauI CCCGC 1 cut(s) 649
Fnu4HI GCNGC 6 cut(s) 234, 241, 258, 483, 517, 654
FokI GGATG 4 cut(s) 95, 401, 432, 734
Fsp4HI GCNGC 6 cut(s) 234, 241, 258, 483, 517, 654
FspBI CTAG 6 cut(s) 18, 33, 42, 200, 543, 641
GlaI GCGC 1 cut(s) 661
GluI GCNGC 6 cut(s) 234, 241, 258, 483, 517, 654
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 1 cut(s) 569
HapII CCGG 2 cut(s) 570, 669
HhaI GCGC 1 cut(s) 662
Hin1I GRCGYC 1 cut(s) 660
Hin1II CATG 2 cut(s) 563, 769
Hin6I GCGC 1 cut(s) 660
HinP1I GCGC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 4 cut(s) 71, 133, 149, 693
HpaII CCGG 2 cut(s) 570, 669
HphI GGTGA 3 cut(s) 16, 359, 499
Hpy188I TCNGA 3 cut(s) 76, 170, 453
Hpy188III TCNNGA 3 cut(s) 137, 157, 707
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 315, 473
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 3 cut(s) 176, 653, 775
HpyF10VI GCNNNNNNNGC 4 cut(s) 190, 482, 659, 781
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 660
Hsp92II CATG 2 cut(s) 563, 769
HspAI GCGC 1 cut(s) 660
KasI GGCGCC 1 cut(s) 659
Kzo9I GATC 4 cut(s) 556, 579, 678, 697
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 4 cut(s) 220, 269, 469, 665
LweI GCATC 1 cut(s) 454
MaeI CTAG 6 cut(s) 18, 33, 42, 200, 543, 641
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 2 cut(s) 329, 761
MalI GATC 4 cut(s) 558, 581, 680, 699
MboI GATC 4 cut(s) 556, 579, 678, 697
MboII GAAGA 8 cut(s) 19, 139, 152, 155, 213, 216, 434, 496
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 478
MluCI AATT 5 cut(s) 90, 113, 249, 593, 635
Mly113I GGCGCC 1 cut(s) 660
MlyI GAGTC 1 cut(s) 687
MmeI TCCRAC 2 cut(s) 193, 285
MnlI CCTC 8 cut(s) 79, 123, 139, 172, 398, 459, 546, 763
MseI TTAA 3 cut(s) 374, 630, 716
MslI CAYNNNNRTG 1 cut(s) 770
MspA1I CMGCKG 1 cut(s) 656
MspCI CTTAAG 1 cut(s) 373
MspI CCGG 2 cut(s) 570, 669
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 190, 482, 659, 781
NarI GGCGCC 1 cut(s) 660
NdeII GATC 4 cut(s) 556, 579, 678, 697
NlaIII CATG 2 cut(s) 563, 769
NlaIV GGNNCC 2 cut(s) 661, 734
NmeAIII GCCGAG 1 cut(s) 286
NruI TCGCGA 1 cut(s) 138
PfeI GAWTC 3 cut(s) 71, 133, 149
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 6 cut(s) 235, 242, 259, 484, 518, 655
PleI GAGTC 1 cut(s) 687
PluTI GGCGCC 1 cut(s) 663
PpsI GAGTC 1 cut(s) 687
PspN4I GGNNCC 2 cut(s) 661, 734
PspPI GGNCC 1 cut(s) 725
PsuI RGATCY 1 cut(s) 579
RruI TCGCGA 1 cut(s) 138
RsaI GTAC 5 cut(s) 22, 547, 553, 689, 749
RsaNI GTAC 5 cut(s) 21, 546, 552, 688, 748
RseI CAYNNNNRTG 1 cut(s) 770
SaqAI TTAA 3 cut(s) 374, 630, 716
SatI GCNGC 6 cut(s) 234, 241, 258, 483, 517, 654
Sau3AI GATC 4 cut(s) 556, 579, 678, 697
Sau96I GGNCC 1 cut(s) 725
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 1 cut(s) 687
SduI GDGCHC 1 cut(s) 478
SetI ASST 8 cut(s) 111, 186, 430, 459, 538, 603, 747, 774
SfaNI GCATC 1 cut(s) 454
SfoI GGCGCC 1 cut(s) 661
SinI GGWCC 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 2 cut(s) 155, 373
SmoI CTYRAG 2 cut(s) 155, 373
SpeI ACTAGT 3 cut(s) 17, 32, 542
Sse9I AATT 5 cut(s) 90, 113, 249, 593, 635
SsiI CCGC 4 cut(s) 241, 281, 517, 656
SspDI GGCGCC 1 cut(s) 659
SspI AATATT 1 cut(s) 357
SspMI CTAG 6 cut(s) 18, 33, 42, 200, 543, 641
StyI CCWWGG 2 cut(s) 325, 728
TaaI ACNGT 2 cut(s) 315, 473
TaiI ACGT 1 cut(s) 603
TaqI TCGA 3 cut(s) 390, 684, 696
TasI AATT 5 cut(s) 90, 113, 249, 593, 635
TatI WGTACW 5 cut(s) 20, 545, 551, 687, 747
TauI GCSGC 2 cut(s) 243, 519
TfiI GAWTC 3 cut(s) 71, 133, 149
Tru1I TTAA 3 cut(s) 374, 630, 716
Tru9I TTAA 3 cut(s) 374, 630, 716
TscAI CASTG 1 cut(s) 294
TseI GCWGC 4 cut(s) 233, 257, 482, 653
TspDTI ATGAA 3 cut(s) 353, 540, 596
TspRI CASTG 1 cut(s) 294
Van91I CCANNNNNTGG 1 cut(s) 350
Vha464I CTTAAG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 725
XapI RAATTY 2 cut(s) 90, 249
XspI CTAG 6 cut(s) 18, 33, 42, 200, 543, 641
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.