MD05G1249600.v1.1

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0020141
Physical Location & Seq
Forward (+)
38117731 .. 38119899
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1249600.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGTTCCAGAATCCTCCAACAGTATCCTCTGCTTTGTGGGAGTCATATCCGAAGGGTGATCGAGTGCTTGCCTCTACCATTGAGTTCAAGCAGCCTCAGTTATCAGCTGCAACATCTTATGGTCTCGGGTTGAAGTTTGAGCATGGAAAGCTTCATAAAGGACAAAGTATATTTGGAGCCATCTTTTCTGCCAGTAGAAAGACCAGAGTAGAACCAACTTACAGGAAGAGAGAAGCATATCCCCAAAATGTGGACCTTCCACCTGTACTACCTAAGAATAAGAAGAAACCGTATCCTATTCCCTTAAATAAGATCAAGCAGGCCGCAAAGGAGGACAAAAAACTTGCAGAATTGGGTATAGAGAAGCCCCTCGAACCTCCAAAAAATGGATTACTTGCCCCTGATCTGATTCCCGTTGCTCACCAAGTACTAGATTCCTGGAAAATTTTAATCAAAGGCATTGCACAGCTGTTGCACGTCATTCCGGTATACGCTTGCACTGAATGCTCTGAGGTTCACGTGAGCCACTCTGGTCATCACATACAGGACTGTCTTGGTGCAACCAGTGCTAAACGCCGGAGCTTCCACTCCTGGATCAAGGGTTCCGTCAATGACGTACTTGTACCCATCGAGGCATATCATCTCTATGACCCTTTTGGTCGGCACATTAAGCATGAAACTATATTTCAATATGACAGGATTCCAGCTGTTGTGGAGCTGTGCATCCAAGCTGGTGTTGAGATCCCTGAGTACCCTTCACGTCGAAGAACCAAACCCATCTGTATGATTGGAAGGAAAGTGATCTATCGAGGTGGATTGATTGAGGAGCCTCAACCAGGTATCTCTGCAAATTCATCATCACTTATTGATCTCGATACACATGGGGCTTGTGGGTGGTTTCCACCTCCACCACCATCAGATATTCCCAAGATTGCACAGGAGACAATGGATGCATATGAAACTGTTAAGCTGGGGGTGACAAAGCTGATGGATAAATACACAGTGAAGGCATGCGGGTATTGCTCCGAGGTTCACGTGGGACCTTGGGGTCACAATGCAAAGCTTTGTGGGGAATTCAAGCACCAATGGAGGGATGGGAAGCATGGCTGGCAGGATGCTACCGTGGATGAAGTTTTCCCTCCAAATTACATATGGTATGTCGAAGACCCCAAAGGGCCTCCGCTGAAGGGCGGTACACTGAAGAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

404

Amino Acids

45.08

Weight (kDa)

8.98

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 74 - 268 2e-99 APO RNA-binding
APO_RNA-bind PF05634 310 - 392 2.1e-13 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020141)

Species Orthologous Gene IDs
malus_domestica MD05G1249600.v1.1 MD10G1230000.v1.1
prunus_persica Prupe.4G114500_v2.0.a1 Prupe.4G114500_v2.0.a1
pyrus_communis pycom05g22690 pycom10g19360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1047
AccB7I CCANNNNNTGG 2 cut(s) 250, 386
AccI GTMKAC 1 cut(s) 489
AciI CCGC 4 cut(s) 324, 1014, 1181, 1191
AclWI GGATC 2 cut(s) 602, 736
AcsI RAATTY 3 cut(s) 444, 850, 1073
AcuI CTGAAG 1 cut(s) 1205
AcvI CACGTG 2 cut(s) 520, 1036
AfaI GTAC 6 cut(s) 267, 429, 618, 624, 752, 1195
AfiI CCNNNNNNNGG 5 cut(s) 250, 386, 836, 1090, 1187
AgsI TTSAA 4 cut(s) 88, 133, 689, 1078
AjiI CACGTC 2 cut(s) 478, 761
AjnI CCWGG 3 cut(s) 437, 590, 835
AloI GAACNNNNNNTCC 2 cut(s) 586, 618
Alw26I GTCTC 2 cut(s) 128, 935
AlwI GGATC 2 cut(s) 602, 736
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 2 cut(s) 321, 1175
ApeKI GCWGC 2 cut(s) 91, 107
ApoI RAATTY 3 cut(s) 444, 850, 1073
Asp700I GAANNNNTTC 1 cut(s) 1205
AspS9I GGNCC 3 cut(s) 253, 1040, 1175
AsuHPI GGTGA 3 cut(s) 68, 413, 988
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 2 cut(s) 253, 1040
BbrPI CACGTG 2 cut(s) 520, 1036
BbsI GAAGAC 1 cut(s) 1170
BbvI GCAGC 2 cut(s) 94, 103
BccI CCATC 6 cut(s) 188, 635, 785, 922, 982, 1088
BciT130I CCWGG 3 cut(s) 439, 592, 837
BciVI GTATCC 2 cut(s) 34, 303
BcoDI GTCTC 2 cut(s) 128, 935
BfaI CTAG 2 cut(s) 431, 1210
BfuI GTATCC 2 cut(s) 34, 303
BisI GCNGC 3 cut(s) 92, 108, 324
BlsI GCNGC 3 cut(s) 93, 109, 325
BmcAI AGTACT 1 cut(s) 429
Bme1390I CCNGG 3 cut(s) 439, 592, 837
Bme18I GGWCC 2 cut(s) 253, 1040
BmeT110I CYCGRG 1 cut(s) 125
BmgBI CACGTC 2 cut(s) 478, 761
BmgT120I GGNCC 3 cut(s) 253, 1040, 1175
BmiI GGNNCC 4 cut(s) 178, 604, 828, 1041
BmrFI CCNGG 3 cut(s) 439, 592, 837
BmsI GCATC 3 cut(s) 732, 940, 1105
BpiI GAAGAC 1 cut(s) 1170
BsaAI YACGTR 2 cut(s) 520, 1036
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 1026, 1043, 1122
BsaWI WCCGGW 1 cut(s) 484
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 386, 836, 1090, 1187
Bse1I ACTGG 2 cut(s) 192, 564
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 3 cut(s) 439, 592, 837
BseDI CCNNGG 3 cut(s) 1026, 1043, 1122
BseGI GGATG 5 cut(s) 723, 955, 1099, 1120, 1132
BseLI CCNNNNNNNGG 5 cut(s) 250, 386, 836, 1090, 1187
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 3 cut(s) 110, 501, 738
BseNI ACTGG 2 cut(s) 192, 564
BseRI GAGGAG 1 cut(s) 839
BseXI GCAGC 2 cut(s) 94, 103
BseYI CCCAGC 1 cut(s) 970
BshFI GGCC 2 cut(s) 323, 1177
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 2 cut(s) 485, 577
BslFI GGGAC 1 cut(s) 1053
BslI CCNNNNNNNGG 5 cut(s) 250, 386, 836, 1090, 1187
BsmAI GTCTC 2 cut(s) 128, 935
BsmFI GGGAC 1 cut(s) 1053
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 2 cut(s) 323, 1177
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
BspACI CCGC 4 cut(s) 324, 1014, 1181, 1191
BspANI GGCC 2 cut(s) 323, 1177
BspCNI CTCAG 3 cut(s) 109, 502, 739
BspLI GGNNCC 4 cut(s) 178, 604, 828, 1041
BspPI GGATC 2 cut(s) 602, 736
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 2 cut(s) 192, 564
BssECI CCNNGG 3 cut(s) 1026, 1043, 1122
BssMI GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
BssNAI GTATAC 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 1043
Bst1107I GTATAC 1 cut(s) 490
Bst2UI CCWGG 3 cut(s) 439, 592, 837
Bst4CI ACNGT 6 cut(s) 22, 291, 551, 964, 1003, 1123
Bst6I CTCTTC 1 cut(s) 221
BstAPI GCANNNNNTGC 2 cut(s) 504, 566
BstBAI YACGTR 2 cut(s) 520, 1036
BstC8I GCNNGC 5 cut(s) 69, 321, 496, 1012, 1109
BstDEI CTNAG 4 cut(s) 96, 273, 510, 747
BstDSI CCRYGG 1 cut(s) 1122
BstENI CCTNNNNNAGG 1 cut(s) 834
BstF5I GGATG 5 cut(s) 723, 955, 1099, 1120, 1132
BstKTI GATC 7 cut(s) 61, 315, 406, 597, 744, 804, 871
BstMAI GTCTC 2 cut(s) 128, 935
BstMBI GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
BstMWI GCNNNNNNNGC 6 cut(s) 148, 504, 566, 670, 1020, 1108
BstNI CCWGG 3 cut(s) 439, 592, 837
BstNSI RCATGY 1 cut(s) 1014
BstSCI CCNGG 3 cut(s) 437, 590, 835
BstV1I GCAGC 2 cut(s) 94, 103
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 1 cut(s) 741
BstYI RGATCY 1 cut(s) 741
BstZ17I GTATAC 1 cut(s) 490
BsuI GTATCC 2 cut(s) 34, 303
BsuRI GGCC 2 cut(s) 323, 1177
BtgI CCRYGG 1 cut(s) 1122
BtrI CACGTC 2 cut(s) 478, 761
BtsCI GGATG 5 cut(s) 723, 955, 1099, 1120, 1132
BtsIMutI CAGTG 4 cut(s) 498, 571, 1008, 1196
Cac8I GCNNGC 5 cut(s) 69, 321, 496, 1012, 1109
Cfr13I GGNCC 3 cut(s) 253, 1040, 1175
CsiI ACCWGGT 1 cut(s) 835
Csp6I GTAC 6 cut(s) 266, 428, 617, 623, 751, 1194
CviAII CATG 5 cut(s) 143, 674, 881, 1011, 1103
CviQI GTAC 6 cut(s) 266, 428, 617, 623, 751, 1194
DdeI CTNAG 4 cut(s) 96, 273, 510, 747
DpnI GATC 7 cut(s) 60, 314, 405, 596, 743, 803, 870
DpnII GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
DrdI GACNNNNNNGTC 1 cut(s) 1047
DseDI GACNNNNNNGTC 1 cut(s) 1047
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 1043
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 253, 1040
Eco57I CTGAAG 1 cut(s) 1205
Eco72I CACGTG 2 cut(s) 520, 1036
Eco88I CYCGRG 1 cut(s) 125
EcoNI CCTNNNNNAGG 1 cut(s) 834
EcoO109I RGGNCCY 2 cut(s) 1040, 1175
EcoRI GAATTC 1 cut(s) 1073
EcoRII CCWGG 3 cut(s) 437, 590, 835
EcoT14I CCWWGG 1 cut(s) 1043
EcoT22I ATGCAT 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 1043
FaeI CATG 5 cut(s) 146, 677, 884, 1014, 1106
FaqI GGGAC 1 cut(s) 1053
FatI CATG 5 cut(s) 142, 673, 880, 1010, 1102
FauI CCCGC 1 cut(s) 1007
FauNDI CATATG 2 cut(s) 955, 1151
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 3 cut(s) 92, 108, 324
FokI GGATG 5 cut(s) 710, 962, 1106, 1127, 1139
Fsp4HI GCNGC 3 cut(s) 92, 108, 324
FspBI CTAG 2 cut(s) 431, 1210
GluI GCNGC 3 cut(s) 92, 108, 324
GsaI CCCAGC 1 cut(s) 974
HaeIII GGCC 2 cut(s) 323, 1177
HapII CCGG 2 cut(s) 485, 577
Hin1II CATG 5 cut(s) 146, 677, 884, 1014, 1106
HindIII AAGCTT 2 cut(s) 149, 1061
HinfI GANTC 5 cut(s) 10, 41, 409, 434, 700
HpaII CCGG 2 cut(s) 485, 577
HphI GGTGA 3 cut(s) 68, 413, 988
Hpy166II GTNNAC 5 cut(s) 253, 490, 517, 1033, 1196
Hpy188I TCNGA 5 cut(s) 51, 408, 511, 919, 1027
Hpy188III TCNNGA 2 cut(s) 7, 872
Hpy8I GTNNAC 5 cut(s) 253, 490, 517, 1033, 1196
Hpy99I CGWCG 1 cut(s) 765
HpyAV CCTTC 6 cut(s) 46, 266, 765, 786, 1000, 1180
HpyCH4III ACNGT 6 cut(s) 22, 291, 551, 964, 1003, 1123
HpyCH4IV ACGT 5 cut(s) 477, 519, 615, 760, 1035
HpyF10VI GCNNNNNNNGC 6 cut(s) 148, 504, 566, 670, 1020, 1108
HpyF3I CTNAG 4 cut(s) 96, 273, 510, 747
HpySE526I ACGT 5 cut(s) 477, 519, 615, 760, 1035
Hsp92II CATG 5 cut(s) 146, 677, 884, 1014, 1106
Kzo9I GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
LmnI GCTCC 5 cut(s) 176, 579, 715, 826, 1028
Lsp1109I GCAGC 2 cut(s) 94, 103
LweI GCATC 3 cut(s) 732, 940, 1105
MabI ACCWGGT 1 cut(s) 835
MaeI CTAG 2 cut(s) 431, 1210
MaeII ACGT 5 cut(s) 477, 519, 615, 760, 1035
MaeIII GTNAC 2 cut(s) 976, 1049
MalI GATC 7 cut(s) 60, 314, 405, 596, 743, 803, 870
MboI GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
MboII GAAGA 4 cut(s) 238, 295, 777, 1175
MflI RGATCY 1 cut(s) 741
MluCI AATT 5 cut(s) 350, 444, 850, 1073, 1144
MlyI GAGTC 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 41
Mph1103I ATGCAT 1 cut(s) 955
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 4 cut(s) 305, 449, 669, 966
MslI CAYNNNNRTG 2 cut(s) 645, 782
MspA1I CMGCKG 4 cut(s) 107, 469, 707, 1183
MspI CCGG 2 cut(s) 485, 577
MspR9I CCNGG 3 cut(s) 439, 592, 837
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 3 cut(s) 439, 592, 837
MwoI GCNNNNNNNGC 6 cut(s) 148, 504, 566, 670, 1020, 1108
NdeI CATATG 2 cut(s) 955, 1151
NdeII GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
NlaIII CATG 5 cut(s) 146, 677, 884, 1014, 1106
NlaIV GGNNCC 4 cut(s) 178, 604, 828, 1041
NmuCI GTSAC 2 cut(s) 976, 1049
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 1 cut(s) 1014
PaeI GCATGC 1 cut(s) 1014
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 4 cut(s) 10, 409, 434, 700
PflMI CCANNNNNTGG 2 cut(s) 250, 386
PfoI TCCNGGA 2 cut(s) 437, 590
PkrI GCNGC 3 cut(s) 93, 109, 325
PleI GAGTC 1 cut(s) 49
PmaCI CACGTG 2 cut(s) 520, 1036
PmlI CACGTG 2 cut(s) 520, 1036
PpsI GAGTC 1 cut(s) 49
Ppu21I YACGTR 2 cut(s) 520, 1036
PpuMI RGGWCCY 1 cut(s) 1040
Psp5II RGGWCCY 1 cut(s) 1040
Psp6I CCWGG 3 cut(s) 437, 590, 835
PspCI CACGTG 2 cut(s) 520, 1036
PspFI CCCAGC 1 cut(s) 970
PspGI CCWGG 3 cut(s) 437, 590, 835
PspN4I GGNNCC 4 cut(s) 178, 604, 828, 1041
PspPI GGNCC 3 cut(s) 253, 1040, 1175
PspPPI RGGWCCY 1 cut(s) 1040
PsuI RGATCY 1 cut(s) 741
PvuII CAGCTG 3 cut(s) 107, 469, 707
RsaI GTAC 6 cut(s) 267, 429, 618, 624, 752, 1195
RsaNI GTAC 6 cut(s) 266, 428, 617, 623, 751, 1194
RseI CAYNNNNRTG 2 cut(s) 645, 782
SaqAI TTAA 4 cut(s) 305, 449, 669, 966
SatI GCNGC 3 cut(s) 92, 108, 324
Sau3AI GATC 7 cut(s) 58, 312, 403, 594, 741, 801, 868
Sau96I GGNCC 3 cut(s) 253, 1040, 1175
ScaI AGTACT 1 cut(s) 429
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 3 cut(s) 439, 592, 837
SexAI ACCWGGT 1 cut(s) 835
SfaNI GCATC 3 cut(s) 732, 940, 1105
SinI GGWCC 2 cut(s) 253, 1040
SmiMI CAYNNNNRTG 2 cut(s) 645, 782
SphI GCATGC 1 cut(s) 1014
Sse9I AATT 5 cut(s) 350, 444, 850, 1073, 1144
SsiI CCGC 4 cut(s) 324, 1014, 1181, 1191
SspMI CTAG 2 cut(s) 431, 1210
StyD4I CCNGG 3 cut(s) 437, 590, 835
StyI CCWWGG 1 cut(s) 1043
TaaI ACNGT 6 cut(s) 22, 291, 551, 964, 1003, 1123
TaiI ACGT 5 cut(s) 480, 522, 618, 763, 1038
TaqI TCGA 7 cut(s) 61, 372, 630, 763, 808, 873, 1161
TasI AATT 5 cut(s) 350, 444, 850, 1073, 1144
TatI WGTACW 2 cut(s) 265, 427
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 4 cut(s) 10, 409, 434, 700
Tru1I TTAA 4 cut(s) 305, 449, 669, 966
Tru9I TTAA 4 cut(s) 305, 449, 669, 966
TscAI CASTG 4 cut(s) 505, 571, 1008, 1203
TseFI GTSAC 2 cut(s) 976, 1049
TseI GCWGC 2 cut(s) 91, 107
Tsp45I GTSAC 2 cut(s) 976, 1049
TspDTI ATGAA 5 cut(s) 143, 690, 843, 972, 1143
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 4 cut(s) 505, 571, 1008, 1203
Van91I CCANNNNNTGG 2 cut(s) 250, 386
VpaK11BI GGWCC 2 cut(s) 253, 1040
XagI CCTNNNNNAGG 1 cut(s) 834
XapI RAATTY 3 cut(s) 444, 850, 1073
XceI RCATGY 1 cut(s) 1014
XcmI CCANNNNNNNNNTGG 2 cut(s) 1091, 1149
XmiI GTMKAC 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 2 cut(s) 431, 1210
ZrmI AGTACT 1 cut(s) 429
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.