Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0020141

Physical Location & Seq

Genomic Coordinates Forward (+)

32689678 .. 32693021

Gene Structure 3344 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1230000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGTTCCAGAATCCTCCAACAGTATCCTCTGCTTTGTGGGAGCCATATCCGAAAGGTGCTTGCCTCTGTATCGATGAGTTCAAGTTGCCTCAGTTATCAGCTGCAAGATCGTACAGTCTTGGGTTGAAGTTTGAGCATGGAAAACTTCATAAAGGCGGAAGTATATTTGGGACTATCTTTGCTGCCAGTAGGAAGACCAGAGTAGAACCAACTTCTAGGAAGCGAGAAGCATATCCCCAGAATGTGGATCTTCCACCTGTGCTACCTAAGAATAAGAAGAAACCGTATCCTATTCCCTTAAAGAAGATCAAGCAGTTCGCAAAGGATGACAAGAAACTTGAAGAATTGGGTATAGAGAAGCCCCTTGAACCTCCAAAAAATGGATTACTTGCCACTGATCTGATTCCTGTTGCTCACCAAGTACTAGATTCCTGGAAAGTTTTAATTAAAGGCATGGCGCAGCTGTTGCACGTCATTCCTGTATACGGATGCACTGAATGCTCTGAGGTTCATGTAAGCCACTCTGGTCATCACATACAGGACTGTCTTGGTTCAACTAGTGCTAAACGCCGAAGCTTCCACTCCTGGATCAAGGGTTCCATCAATGACGTACTTGTACCCATCGAGGCATATCATCTCTTTGACCCTTTTGGTCGACGCATTAAGCATGAAACTAGATTTCAATACGACAGGATTCCAGCTGTTGTGGAGCTGTGCATCCAAGCTGGTGTTGAGATCCCCGAGTACCCTTCACGTCGAAGGACCAAACCCATCCGGATGATTGGAAGGAAAGTGATAGATCGAGGTGGATTGCTAGAGGAGCCTCAACCAGGGTGCTCTGCAGATCCATCTTCCCTTGTTGATCTCGACACACATGGGGCTTGTGAGCGGTTTCCACCTCCACTGCCAACGGATATTCCCAGGATTGCACAGGATACAATGGATGCATATGAAACTGTTAGGTTGGGGGTGACAAAGCTGATGAAGAAATACACTGTGAAGGCATGCGGGTATTGCTCCGAGGTTCATGTGGGACCTTGGGGTCACAATGCAAAGCTTTGTGGGGAATTCAAGCACCAATGGAGGGATGGGAAGCATGGCTGGCAGGATGCTACCGTGGACGAGGTTTTACCTCCAAATTATGTATGGCACGTCGAAGACCCCAAAGGGCCTCCACTGAAGGGCGGTGCACTCAAGAAGTTCTACGGGAAGGCTCCTGCAGTGGTAGAGGTTTGCTTGCAGGCAGGTGCACGGATCCCTGAGAAATATAAACCCATGATGAGGCTTGACATTGTAGTCCCTAAAAGTGAGGAGGCACAATTAGTTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

APO_RNA-bind

Protein Analysis

444

Amino Acids

49.6

Weight (kDa)

9.11

Isoelectric Point (pI)

44.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APO_RNA-bind	PF05634	72 - 267	5.5e-102	APO RNA-binding
APO_RNA-bind	PF05634	308 - 421	7.4e-22	APO RNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020141)

Species	Orthologous Gene IDs
malus_domestica	MD05G1249600.v1.1 MD10G1230000.v1.1
prunus_persica	Prupe.4G114500_v2.0.a1 Prupe.4G114500_v2.0.a1
pyrus_communis	pycom05g22690 pycom10g19360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1235
AasI	GACNNNNNNGTC	2 cut(s)	1041, 1295
Acc36I	ACCTGC	1 cut(s)	1235
AccB7I	CCANNNNNTGG	2 cut(s)	244, 380
AccBSI	CCGCTC	1 cut(s)	889
AccI	GTMKAC	2 cut(s)	483, 655
AccIII	TCCGGA	1 cut(s)	774
AciI	CCGC	4 cut(s)	156, 889, 1008, 1185
AclWI	GGATC	6 cut(s)	255, 596, 730, 839, 1249, 1262
AcsI	RAATTY	1 cut(s)	1067
AcuI	CTGAAG	1 cut(s)	1199
AfaI	GTAC	5 cut(s)	113, 423, 612, 618, 746
AfiI	CCNNNNNNNGG	7 cut(s)	244, 380, 485, 830, 1084, 1181, 1281
AgsI	TTSAA	7 cut(s)	82, 127, 341, 368, 555, 683, 1072
AhlI	ACTAGT	1 cut(s)	557
AjiI	CACGTC	3 cut(s)	472, 755, 1153
AjnI	CCWGG	4 cut(s)	431, 584, 829, 920
AloI	GAACNNNNNNTCC	2 cut(s)	580, 612
AluBI	AGCT	8 cut(s)	101, 463, 576, 701, 712, 725, 979, 1057
AluI	AGCT	8 cut(s)	101, 463, 576, 701, 712, 725, 979, 1057
Alw21I	GWGCWC	3 cut(s)	839, 1192, 1252
Alw44I	GTGCAC	2 cut(s)	1188, 1248
AlwI	GGATC	6 cut(s)	255, 596, 730, 839, 1249, 1262
Ama87I	CYCGRG	1 cut(s)	740
Aor13HI	TCCGGA	1 cut(s)	774
AoxI	GGCC	1 cut(s)	1169
ApaLI	GTGCAC	2 cut(s)	1188, 1248
ApeKI	GCWGC	3 cut(s)	101, 182, 460
ApoI	RAATTY	1 cut(s)	1067
AspLEI	GCGC	1 cut(s)	460
AspS9I	GGNCC	3 cut(s)	762, 1034, 1169
AsuHPI	GGTGA	2 cut(s)	407, 982
AvaI	CYCGRG	1 cut(s)	740
AvaII	GGWCC	2 cut(s)	762, 1034
BaeGI	GKGCMC	2 cut(s)	1192, 1252
BamHI	GGATCC	1 cut(s)	1254
BbsI	GAAGAC	2 cut(s)	200, 1164
Bbv12I	GWGCWC	3 cut(s)	839, 1192, 1252
BbvI	GCAGC	3 cut(s)	88, 169, 472
BccI	CCATC	5 cut(s)	608, 629, 779, 856, 1082
BciT130I	CCWGG	4 cut(s)	433, 586, 831, 922
BciVI	GTATCC	3 cut(s)	34, 297, 928
BcuI	ACTAGT	1 cut(s)	557
BfaI	CTAG	5 cut(s)	216, 425, 558, 675, 815
BfmI	CTRYAG	2 cut(s)	840, 1218
BfuAI	ACCTGC	1 cut(s)	1235
BfuI	GTATCC	3 cut(s)	34, 297, 928
BisI	GCNGC	3 cut(s)	102, 183, 461
BlsI	GCNGC	3 cut(s)	103, 184, 462
BmcAI	AGTACT	1 cut(s)	423
Bme1390I	CCNGG	4 cut(s)	433, 586, 831, 922
Bme18I	GGWCC	2 cut(s)	762, 1034
BmeT110I	CYCGRG	1 cut(s)	740
BmgBI	CACGTC	3 cut(s)	472, 755, 1153
BmgT120I	GGNCC	3 cut(s)	762, 1034, 1169
BmiI	GGNNCC	6 cut(s)	42, 598, 822, 1035, 1215, 1256
BmrFI	CCNGG	4 cut(s)	433, 586, 831, 922
BmsI	GCATC	4 cut(s)	479, 726, 934, 1099
BpiI	GAAGAC	2 cut(s)	200, 1164
BpuEI	CTTGAG	1 cut(s)	1178
Bsa29I	ATCGAT	1 cut(s)	72
BsaJI	CCNNGG	5 cut(s)	830, 920, 1020, 1037, 1116
BsaWI	WCCGGW	1 cut(s)	774
Bsc4I	CCNNNNNNNGG	7 cut(s)	244, 380, 485, 830, 1084, 1181, 1281
Bse1I	ACTGG	1 cut(s)	186
BseAI	TCCGGA	1 cut(s)	774
BseBI	CCWGG	4 cut(s)	433, 586, 831, 922
BseCI	ATCGAT	1 cut(s)	72
BseDI	CCNNGG	5 cut(s)	830, 920, 1020, 1037, 1116
BseGI	GGATG	8 cut(s)	331, 494, 717, 771, 783, 949, 1093, 1114
BseLI	CCNNNNNNNGG	7 cut(s)	244, 380, 485, 830, 1084, 1181, 1281
BseMII	CTCAG	3 cut(s)	104, 495, 1251
BseNI	ACTGG	1 cut(s)	186
BseRI	GAGGAG	2 cut(s)	833, 1325
BseSI	GKGCMC	2 cut(s)	1192, 1252
BseXI	GCAGC	3 cut(s)	88, 169, 472
BshFI	GGCC	1 cut(s)	1171
BshVI	ATCGAT	1 cut(s)	72
BsiHKAI	GWGCWC	3 cut(s)	839, 1192, 1252
BsiHKCI	CYCGRG	1 cut(s)	740
BsiSI	CCGG	1 cut(s)	775
BslFI	GGGAC	3 cut(s)	184, 1047, 1283
BslI	CCNNNNNNNGG	7 cut(s)	244, 380, 485, 830, 1084, 1181, 1281
BsmFI	GGGAC	3 cut(s)	184, 1047, 1283
BsmI	GAATGC	1 cut(s)	503
BsnI	GGCC	1 cut(s)	1171
BsoBI	CYCGRG	1 cut(s)	740
Bsp1286I	GDGCHC	3 cut(s)	839, 1192, 1252
Bsp13I	TCCGGA	1 cut(s)	774
BspACI	CCGC	4 cut(s)	156, 889, 1008, 1185
BspANI	GGCC	1 cut(s)	1171
BspCNI	CTCAG	3 cut(s)	103, 496, 1252
BspDI	ATCGAT	1 cut(s)	72
BspEI	TCCGGA	1 cut(s)	774
BspLI	GGNNCC	6 cut(s)	42, 598, 822, 1035, 1215, 1256
BspMAI	CTGCAG	2 cut(s)	844, 1222
BspMI	ACCTGC	1 cut(s)	1235
BspPI	GGATC	6 cut(s)	255, 596, 730, 839, 1249, 1262
BsrBI	CCGCTC	1 cut(s)	889
BsrI	ACTGG	1 cut(s)	186
BssECI	CCNNGG	5 cut(s)	830, 920, 1020, 1037, 1116
BssNAI	GTATAC	1 cut(s)	484
BssT1I	CCWWGG	1 cut(s)	1037
Bst1107I	GTATAC	1 cut(s)	484
Bst2UI	CCWGG	4 cut(s)	433, 586, 831, 922
Bst4CI	ACNGT	7 cut(s)	22, 116, 285, 545, 958, 997, 1117
BstAPI	GCANNNNNTGC	2 cut(s)	466, 498
BstC8I	GCNNGC	5 cut(s)	61, 1006, 1103, 1238, 1242
BstDEI	CTNAG	4 cut(s)	90, 267, 504, 1260
BstDSI	CCRYGG	1 cut(s)	1116
BstENI	CCTNNNNNAGG	1 cut(s)	828
BstF5I	GGATG	8 cut(s)	331, 494, 717, 771, 783, 949, 1093, 1114
BstHHI	GCGC	1 cut(s)	460
BstMWI	GCNNNNNNNGC	5 cut(s)	466, 498, 820, 1014, 1102
BstNI	CCWGG	4 cut(s)	433, 586, 831, 922
BstNSI	RCATGY	1 cut(s)	1008
BstSCI	CCNGG	4 cut(s)	431, 584, 829, 920
BstSFI	CTRYAG	2 cut(s)	840, 1218
BstSLI	GKGCMC	2 cut(s)	1192, 1252
BstV1I	GCAGC	3 cut(s)	88, 169, 472
BstV2I	GAAGAC	2 cut(s)	200, 1164
BstX2I	RGATCY	4 cut(s)	247, 735, 844, 1254
BstYI	RGATCY	4 cut(s)	247, 735, 844, 1254
BstZ17I	GTATAC	1 cut(s)	484
Bsu15I	ATCGAT	1 cut(s)	72
BsuI	GTATCC	3 cut(s)	34, 297, 928
BsuRI	GGCC	1 cut(s)	1171
BsuTUI	ATCGAT	1 cut(s)	72
BtgI	CCRYGG	1 cut(s)	1116
BtrI	CACGTC	3 cut(s)	472, 755, 1153
BtsCI	GGATG	8 cut(s)	331, 494, 717, 771, 783, 949, 1093, 1114
BtsI	GCAGTG	2 cut(s)	902, 1227
BtsIMutI	CAGTG	6 cut(s)	393, 492, 902, 993, 1175, 1227
BveI	ACCTGC	1 cut(s)	1235
Cac8I	GCNNGC	5 cut(s)	61, 1006, 1103, 1238, 1242
CfoI	GCGC	1 cut(s)	460
Cfr13I	GGNCC	3 cut(s)	762, 1034, 1169
ClaI	ATCGAT	1 cut(s)	72
CseI	GACGC	1 cut(s)	666
Csp6I	GTAC	5 cut(s)	112, 422, 611, 617, 745
CviAII	CATG	9 cut(s)	137, 454, 512, 668, 875, 1005, 1028, 1097, 1276
CviQI	GTAC	5 cut(s)	112, 422, 611, 617, 745
DdeI	CTNAG	4 cut(s)	90, 267, 504, 1260
DrdI	GACNNNNNNGTC	2 cut(s)	1041, 1295
DseDI	GACNNNNNNGTC	2 cut(s)	1041, 1295
EciI	GGCGGA	1 cut(s)	171
Eco130I	CCWWGG	1 cut(s)	1037
Eco47I	GGWCC	2 cut(s)	762, 1034
Eco57I	CTGAAG	1 cut(s)	1199
Eco88I	CYCGRG	1 cut(s)	740
EcoNI	CCTNNNNNAGG	1 cut(s)	828
EcoO109I	RGGNCCY	2 cut(s)	1034, 1169
EcoRI	GAATTC	1 cut(s)	1067
EcoRII	CCWGG	4 cut(s)	431, 584, 829, 920
EcoT14I	CCWWGG	1 cut(s)	1037
EcoT22I	ATGCAT	1 cut(s)	949
ErhI	CCWWGG	1 cut(s)	1037
FaeI	CATG	9 cut(s)	140, 457, 515, 671, 878, 1008, 1031, 1100, 1279
FaqI	GGGAC	3 cut(s)	184, 1047, 1283
FatI	CATG	9 cut(s)	136, 453, 511, 667, 874, 1004, 1027, 1096, 1275
FauI	CCCGC	1 cut(s)	1001
FauNDI	CATATG	1 cut(s)	949
FblI	GTMKAC	2 cut(s)	483, 655
Fnu4HI	GCNGC	3 cut(s)	102, 183, 461
FokI	GGATG	8 cut(s)	338, 501, 704, 758, 790, 956, 1100, 1121
Fsp4HI	GCNGC	3 cut(s)	102, 183, 461
FspBI	CTAG	5 cut(s)	216, 425, 558, 675, 815
GlaI	GCGC	1 cut(s)	459
GluI	GCNGC	3 cut(s)	102, 183, 461
HaeIII	GGCC	1 cut(s)	1171
HapII	CCGG	1 cut(s)	775
HgaI	GACGC	1 cut(s)	666
HhaI	GCGC	1 cut(s)	460
Hin1II	CATG	9 cut(s)	140, 457, 515, 671, 878, 1008, 1031, 1100, 1279
Hin6I	GCGC	1 cut(s)	458
HinP1I	GCGC	1 cut(s)	458
HincII	GTYRAC	1 cut(s)	656
HindII	GTYRAC	1 cut(s)	656
HindIII	AAGCTT	2 cut(s)	574, 1055
HinfI	GANTC	4 cut(s)	10, 403, 428, 694
HpaII	CCGG	1 cut(s)	775
HphI	GGTGA	2 cut(s)	407, 982
Hpy166II	GTNNAC	5 cut(s)	484, 656, 1120, 1190, 1250
Hpy188I	TCNGA	4 cut(s)	51, 402, 505, 1021
Hpy188III	TCNNGA	4 cut(s)	7, 775, 866, 1195
Hpy8I	GTNNAC	5 cut(s)	484, 656, 1120, 1190, 1250
Hpy99I	CGWCG	3 cut(s)	660, 759, 1157
HpyAV	CCTTC	6 cut(s)	753, 759, 780, 994, 1174, 1204
HpyCH4III	ACNGT	7 cut(s)	22, 116, 285, 545, 958, 997, 1117
HpyCH4IV	ACGT	4 cut(s)	471, 609, 754, 1152
HpyF10VI	GCNNNNNNNGC	5 cut(s)	466, 498, 820, 1014, 1102
HpyF3I	CTNAG	4 cut(s)	90, 267, 504, 1260
HpySE526I	ACGT	4 cut(s)	471, 609, 754, 1152
Hsp92II	CATG	9 cut(s)	140, 457, 515, 671, 878, 1008, 1031, 1100, 1279
HspAI	GCGC	1 cut(s)	458
Kpn2I	TCCGGA	1 cut(s)	774
LmnI	GCTCC	5 cut(s)	40, 709, 820, 1022, 1219
Lsp1109I	GCAGC	3 cut(s)	88, 169, 472
LweI	GCATC	4 cut(s)	479, 726, 934, 1099
MaeI	CTAG	5 cut(s)	216, 425, 558, 675, 815
MaeII	ACGT	4 cut(s)	471, 609, 754, 1152
MaeIII	GTNAC	2 cut(s)	970, 1043
MbiI	CCGCTC	1 cut(s)	889
MboII	GAAGA	8 cut(s)	205, 242, 289, 316, 353, 843, 997, 1169
MflI	RGATCY	4 cut(s)	247, 735, 844, 1254
MhlI	GDGCHC	3 cut(s)	839, 1192, 1252
MluCI	AATT	5 cut(s)	344, 444, 1067, 1138, 1319
MmeI	TCCRAC	1 cut(s)	41
Mph1103I	ATGCAT	1 cut(s)	949
MroI	TCCGGA	1 cut(s)	774
MseI	TTAA	4 cut(s)	299, 443, 447, 663
MslI	CAYNNNNRTG	1 cut(s)	776
MspA1I	CMGCKG	3 cut(s)	101, 463, 701
MspI	CCGG	1 cut(s)	775
MspR9I	CCNGG	4 cut(s)	433, 586, 831, 922
Mva1269I	GAATGC	1 cut(s)	503
MvaI	CCWGG	4 cut(s)	433, 586, 831, 922
MwoI	GCNNNNNNNGC	5 cut(s)	466, 498, 820, 1014, 1102
NdeI	CATATG	1 cut(s)	949
NlaIII	CATG	9 cut(s)	140, 457, 515, 671, 878, 1008, 1031, 1100, 1279
NlaIV	GGNNCC	6 cut(s)	42, 598, 822, 1035, 1215, 1256
NmuCI	GTSAC	2 cut(s)	970, 1043
NsiI	ATGCAT	1 cut(s)	949
NspI	RCATGY	1 cut(s)	1008
PacI	TTAATTAA	1 cut(s)	447
PaeI	GCATGC	1 cut(s)	1008
PaqCI	CACCTGC	1 cut(s)	1235
PctI	GAATGC	1 cut(s)	503
PfeI	GAWTC	4 cut(s)	10, 403, 428, 694
PflMI	CCANNNNNTGG	2 cut(s)	244, 380
PfoI	TCCNGGA	2 cut(s)	431, 584
PkrI	GCNGC	3 cut(s)	103, 184, 462
PpuMI	RGGWCCY	1 cut(s)	1034
Psp5II	RGGWCCY	1 cut(s)	1034
Psp6I	CCWGG	4 cut(s)	431, 584, 829, 920
PspGI	CCWGG	4 cut(s)	431, 584, 829, 920
PspN4I	GGNNCC	6 cut(s)	42, 598, 822, 1035, 1215, 1256
PspPI	GGNCC	3 cut(s)	762, 1034, 1169
PspPPI	RGGWCCY	1 cut(s)	1034
PstI	CTGCAG	2 cut(s)	844, 1222
PsuI	RGATCY	4 cut(s)	247, 735, 844, 1254
PvuII	CAGCTG	3 cut(s)	101, 463, 701
RsaI	GTAC	5 cut(s)	113, 423, 612, 618, 746
RsaNI	GTAC	5 cut(s)	112, 422, 611, 617, 745
RseI	CAYNNNNRTG	1 cut(s)	776
SalI	GTCGAC	1 cut(s)	654
SaqAI	TTAA	4 cut(s)	299, 443, 447, 663
SatI	GCNGC	3 cut(s)	102, 183, 461
Sau96I	GGNCC	3 cut(s)	762, 1034, 1169
ScaI	AGTACT	1 cut(s)	423
ScrFI	CCNGG	4 cut(s)	433, 586, 831, 922
SduI	GDGCHC	3 cut(s)	839, 1192, 1252
SfaNI	GCATC	4 cut(s)	479, 726, 934, 1099
SfcI	CTRYAG	2 cut(s)	840, 1218
SinI	GGWCC	2 cut(s)	762, 1034
SmiMI	CAYNNNNRTG	1 cut(s)	776
SmlI	CTYRAG	1 cut(s)	1193
SmoI	CTYRAG	1 cut(s)	1193
SpeI	ACTAGT	1 cut(s)	557
SphI	GCATGC	1 cut(s)	1008
Sse9I	AATT	5 cut(s)	344, 444, 1067, 1138, 1319
SsiI	CCGC	4 cut(s)	156, 889, 1008, 1185
SspMI	CTAG	5 cut(s)	216, 425, 558, 675, 815
StyD4I	CCNGG	4 cut(s)	431, 584, 829, 920
StyI	CCWWGG	1 cut(s)	1037
TaaI	ACNGT	7 cut(s)	22, 116, 285, 545, 958, 997, 1117
TaiI	ACGT	4 cut(s)	474, 612, 757, 1155
TaqI	TCGA	7 cut(s)	72, 624, 655, 757, 802, 867, 1155
TasI	AATT	5 cut(s)	344, 444, 1067, 1138, 1319
TatI	WGTACW	1 cut(s)	421
TfiI	GAWTC	4 cut(s)	10, 403, 428, 694
Tru1I	TTAA	4 cut(s)	299, 443, 447, 663
Tru9I	TTAA	4 cut(s)	299, 443, 447, 663
TscAI	CASTG	6 cut(s)	400, 499, 909, 1000, 1182, 1227
TseFI	GTSAC	2 cut(s)	970, 1043
TseI	GCWGC	3 cut(s)	101, 182, 460
Tsp45I	GTSAC	2 cut(s)	970, 1043
TspDTI	ATGAA	6 cut(s)	137, 500, 684, 966, 998, 1016
TspGWI	ACGGA	3 cut(s)	501, 926, 1267
TspRI	CASTG	6 cut(s)	400, 499, 909, 1000, 1182, 1227
Van91I	CCANNNNNTGG	2 cut(s)	244, 380
VneI	GTGCAC	2 cut(s)	1188, 1248
VpaK11BI	GGWCC	2 cut(s)	762, 1034
XagI	CCTNNNNNAGG	1 cut(s)	828
XapI	RAATTY	1 cut(s)	1067
XceI	RCATGY	1 cut(s)	1008
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1085, 1143
XmiI	GTMKAC	2 cut(s)	483, 655
XspI	CTAG	5 cut(s)	216, 425, 558, 675, 815
ZrmI	AGTACT	1 cut(s)	423
Zsp2I	ATGCAT	1 cut(s)	949

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.