Prupe.4G114500_v2.0.a1

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0020141
Physical Location & Seq
Reverse (-)
6113642 .. 6117428
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G114500.1

Sequence Viewer

Length: 1332 bp
ATGTTCCATAAGCCTCCAACAGTATCCTCTGCTTTGTGGGAACCATATCAGAAAGGTGCTTGCCTCTGTACCGTTGAGTTCAAGCGAACTCAACTATCAGCTGCGAGTTCATATAGTGTCGGGTTCAAGTTTGAGCATGGAAAGCTTCATAAAGGAGGAAGTATTTTGGGAACTATCTTTTCTGCCAGTAGGAAGCCCAGGGTAGAACCAACCTTAAGGAAGCGAGAAACATATCCCCAAAATGTGGATCTTCCACCCGTACTACCTAAGCAAAAGAAGAAACCCTATCCTATTCCCTTCAAGAAGATCAAGCAGGTTGCAAAGAAGGACAAAAAACTTGCAGAAATGGGTATAGAGAAGCCCCTCGACCCTCCGAAAAATGGATTACTTGCCCCTGATTTAATTCCTGTTGCTTACCAAGTACTAGATGCGTGGAAAGTTTTAATTAAAGGCCTTGGCCAGCTGTTGTATGTTATTCCTGTATACGGCTGCAATGAATGCTCTGAGGTTCATGTGAGCCACTCTGGTCATCACATGCAGGACTGTCTTGGCCCAACCAATTCCAAGCGCCGAAGCTTCCATTCCTGGATTAAGGGTTCTATCAATGACATACTTGTACCCATCGAGGCATATCATCTGTATGATCCTTTTGGTCGGCGCATTAAACATGAAACTAGATTTCAATATGACAGGATTCCAGCTATCGTGGAGCTGTGCATCCAAGCTGGTGTGGAAATACCGGAGTACCCTTCACGTCGAAGAACCAAACCTATCCGAATGATTGGAAGGAAAGTAATTGATCGTGGTGGATTGGTTGAGGAGCCACAGCCATGGCGCGCTGCGAATCCATCTTCACTTGTTGATCTCGATACACATGGGGCTTGTGAGCGGTTTCCACCTCCCCTGCCATCAGACATTCCCAAGATTGCACAGGAGACAATGGATGCATATGAAACTGTGAGGTTCGGGGTCACGAAGCTGATGAAGAAATACACTGTGAAGGCATGTGGGTATTGCACAGAGGTTCATGTTGGACCTTGGGGTCACAACGCAAAGCTTTGTGGGGAATTCAAGCACCAATGGAGGGATGGGAAGCATGGTTGGCAAGACGCTACCGTGGATGAAGTTTTCCCACCAAACTATGTATGGCATGTCAAAGACCCTAAAGGGCCTCCAATGAAAGGGGGTGCACTCAAGAAGTTCTATGGGAAGGCTCCTGCGGTGGTAGAGGTATGCTTGCAGGCAGGTGCACAGATCCCTGAGAAATACAAACCCATGATGAGGCTTGACATTGTAGTCCCTGACAGTGAGGAGGCACTGTTAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

444

Amino Acids

49.91

Weight (kDa)

9.36

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020141)

Species Orthologous Gene IDs
malus_domestica MD05G1249600.v1.1 MD10G1230000.v1.1
prunus_persica Prupe.4G114500_v2.0.a1 Prupe.4G114500_v2.0.a1
pyrus_communis pycom05g22690 pycom10g19360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1235
AasI GACNNNNNNGTC 2 cut(s) 1041, 1295
Acc36I ACCTGC 2 cut(s) 304, 1235
AccB7I CCANNNNNTGG 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 889
AccI GTMKAC 1 cut(s) 483
AccII CGCG 1 cut(s) 837
AciI CCGC 2 cut(s) 889, 1220
AclWI GGATC 3 cut(s) 255, 638, 1249
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 1 cut(s) 1067
AfaI GTAC 5 cut(s) 70, 261, 423, 618, 746
AfiI CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1084, 1181, 1281
AflII CTTAAG 1 cut(s) 214
AgsI TTSAA 5 cut(s) 82, 127, 301, 683, 1072
AjiI CACGTC 1 cut(s) 755
AjnI CCWGG 2 cut(s) 197, 584
AloI GAACNNNNNNTCC 2 cut(s) 580, 612
AluBI AGCT 9 cut(s) 101, 145, 463, 576, 701, 712, 725, 979, 1057
AluI AGCT 9 cut(s) 101, 145, 463, 576, 701, 712, 725, 979, 1057
Alw21I GWGCWC 2 cut(s) 1192, 1252
Alw26I GTCTC 1 cut(s) 929
Alw44I GTGCAC 2 cut(s) 1188, 1248
AlwI GGATC 3 cut(s) 255, 638, 1249
AoxI GGCC 4 cut(s) 451, 457, 550, 1169
ApaLI GTGCAC 2 cut(s) 1188, 1248
ApeKI GCWGC 3 cut(s) 101, 489, 839
ApoI RAATTY 1 cut(s) 1067
AspLEI GCGC 4 cut(s) 570, 660, 837, 839
AspS9I GGNCC 3 cut(s) 551, 1034, 1169
AvaII GGWCC 1 cut(s) 1034
BaeGI GKGCMC 2 cut(s) 1192, 1252
BaeI ACNNNNGTAYC 2 cut(s) 728, 761
BalI TGGCCA 1 cut(s) 459
Bbv12I GWGCWC 2 cut(s) 1192, 1252
BbvI GCAGC 3 cut(s) 88, 476, 826
BccI CCATC 4 cut(s) 629, 856, 916, 1082
BceAI ACGGC 1 cut(s) 502
BciT130I CCWGG 2 cut(s) 199, 586
BciVI GTATCC 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 929
BfaI CTAG 2 cut(s) 425, 675
BfoI RGCGCY 1 cut(s) 571
BfrI CTTAAG 1 cut(s) 214
BfuAI ACCTGC 2 cut(s) 304, 1235
BfuI GTATCC 1 cut(s) 34
BisI GCNGC 3 cut(s) 102, 490, 840
BlsI GCNGC 3 cut(s) 103, 491, 841
BmcAI AGTACT 1 cut(s) 423
Bme1390I CCNGG 2 cut(s) 199, 586
Bme18I GGWCC 1 cut(s) 1034
BmgBI CACGTC 1 cut(s) 755
BmgT120I GGNCC 3 cut(s) 551, 1034, 1169
BmiI GGNNCC 3 cut(s) 42, 822, 1215
BmrFI CCNGG 2 cut(s) 199, 586
BmsI GCATC 3 cut(s) 418, 726, 934
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 1178
BsaJI CCNNGG 6 cut(s) 197, 198, 454, 830, 1037, 1116
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 1 cut(s) 1304
Bsc4I CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1084, 1181, 1281
Bse1I ACTGG 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 2 cut(s) 199, 586
BseDI CCNNGG 6 cut(s) 197, 198, 454, 830, 1037, 1116
BseGI GGATG 4 cut(s) 717, 949, 1093, 1126
BseLI CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1084, 1181, 1281
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 2 cut(s) 495, 1251
BseNI ACTGG 1 cut(s) 186
BsePI GCGCGC 1 cut(s) 835
BseRI GAGGAG 2 cut(s) 833, 1325
BseSI GKGCMC 2 cut(s) 1192, 1252
BseXI GCAGC 3 cut(s) 88, 476, 826
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 4 cut(s) 453, 459, 552, 1171
BsiHKAI GWGCWC 2 cut(s) 1192, 1252
BsiSI CCGG 1 cut(s) 740
BslFI GGGAC 1 cut(s) 1283
BslI CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1084, 1181, 1281
BsmAI GTCTC 1 cut(s) 929
BsmFI GGGAC 1 cut(s) 1283
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 4 cut(s) 453, 459, 552, 1171
Bsp1286I GDGCHC 2 cut(s) 1192, 1252
Bsp143I GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 2 cut(s) 889, 1220
BspANI GGCC 4 cut(s) 453, 459, 552, 1171
BspCNI CTCAG 2 cut(s) 496, 1252
BspFNI CGCG 1 cut(s) 837
BspLI GGNNCC 3 cut(s) 42, 822, 1215
BspMI ACCTGC 2 cut(s) 304, 1235
BspPI GGATC 3 cut(s) 255, 638, 1249
BspTI CTTAAG 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 889
BsrDI GCAATG 1 cut(s) 499
BsrI ACTGG 1 cut(s) 186
BssECI CCNNGG 6 cut(s) 197, 198, 454, 830, 1037, 1116
BssHII GCGCGC 1 cut(s) 835
BssMI GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
BssNAI GTATAC 1 cut(s) 484
BssT1I CCWWGG 3 cut(s) 454, 830, 1037
Bst1107I GTATAC 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 199, 586
Bst4CI ACNGT 8 cut(s) 22, 73, 545, 958, 997, 1117, 1307, 1320
BstAFI CTTAAG 1 cut(s) 214
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 5 cut(s) 61, 461, 837, 1238, 1242
BstDEI CTNAG 3 cut(s) 267, 504, 1260
BstDSI CCRYGG 2 cut(s) 830, 1116
BstF5I GGATG 4 cut(s) 717, 949, 1093, 1126
BstFNI CGCG 1 cut(s) 837
BstH2I RGCGCY 1 cut(s) 571
BstHHI GCGC 4 cut(s) 570, 660, 837, 839
BstKTI GATC 6 cut(s) 250, 309, 646, 802, 865, 1257
BstMAI GTCTC 1 cut(s) 929
BstMBI GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
BstMWI GCNNNNNNNGC 3 cut(s) 142, 498, 1102
BstNI CCWGG 2 cut(s) 199, 586
BstNSI RCATGY 3 cut(s) 538, 1008, 1154
BstSCI CCNGG 2 cut(s) 197, 584
BstSLI GKGCMC 2 cut(s) 1192, 1252
BstUI CGCG 1 cut(s) 837
BstV1I GCAGC 3 cut(s) 88, 476, 826
BstX2I RGATCY 2 cut(s) 247, 1254
BstXI CCANNNNNNTGG 1 cut(s) 831
BstYI RGATCY 2 cut(s) 247, 1254
BstZ17I GTATAC 1 cut(s) 484
BsuI GTATCC 1 cut(s) 34
BsuRI GGCC 4 cut(s) 453, 459, 552, 1171
BtgI CCRYGG 2 cut(s) 830, 1116
BtrI CACGTC 1 cut(s) 755
BtsCI GGATG 4 cut(s) 717, 949, 1093, 1126
BtsIMutI CAGTG 3 cut(s) 993, 1312, 1316
BveI ACCTGC 2 cut(s) 304, 1235
Cac8I GCNNGC 5 cut(s) 61, 461, 837, 1238, 1242
CfoI GCGC 4 cut(s) 570, 660, 837, 839
Cfr13I GGNCC 3 cut(s) 551, 1034, 1169
CseI GACGC 1 cut(s) 1118
Csp6I GTAC 5 cut(s) 69, 260, 422, 617, 745
CviQI GTAC 5 cut(s) 69, 260, 422, 617, 745
DdeI CTNAG 3 cut(s) 267, 504, 1260
DpnI GATC 6 cut(s) 249, 308, 645, 801, 864, 1256
DpnII GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
DrdI GACNNNNNNGTC 2 cut(s) 1041, 1295
DseDI GACNNNNNNGTC 2 cut(s) 1041, 1295
EaeI YGGCCR 1 cut(s) 457
Eco130I CCWWGG 3 cut(s) 454, 830, 1037
Eco147I AGGCCT 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 1034
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRI GAATTC 1 cut(s) 1067
EcoRII CCWGG 2 cut(s) 197, 584
EcoT14I CCWWGG 3 cut(s) 454, 830, 1037
EcoT22I ATGCAT 1 cut(s) 949
ErhI CCWWGG 3 cut(s) 454, 830, 1037
FaqI GGGAC 1 cut(s) 1283
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 3 cut(s) 102, 490, 840
FokI GGATG 4 cut(s) 704, 956, 1100, 1133
Fsp4HI GCNGC 3 cut(s) 102, 490, 840
FspBI CTAG 2 cut(s) 425, 675
GlaI GCGC 4 cut(s) 569, 659, 836, 838
GluI GCNGC 3 cut(s) 102, 490, 840
HaeII RGCGCY 1 cut(s) 571
HaeIII GGCC 4 cut(s) 453, 459, 552, 1171
HapII CCGG 1 cut(s) 740
HgaI GACGC 1 cut(s) 1118
HhaI GCGC 4 cut(s) 570, 660, 837, 839
Hin6I GCGC 4 cut(s) 568, 658, 835, 837
HinP1I GCGC 4 cut(s) 568, 658, 835, 837
HindIII AAGCTT 3 cut(s) 143, 574, 1055
HinfI GANTC 2 cut(s) 694, 844
HpaII CCGG 1 cut(s) 740
Hpy166II GTNNAC 3 cut(s) 484, 1190, 1250
Hpy188I TCNGA 5 cut(s) 51, 375, 505, 776, 913
Hpy188III TCNNGA 4 cut(s) 301, 866, 973, 1195
Hpy8I GTNNAC 3 cut(s) 484, 1190, 1250
Hpy99I CGWCG 1 cut(s) 759
HpyAV CCTTC 6 cut(s) 307, 319, 759, 780, 994, 1204
HpyCH4III ACNGT 8 cut(s) 22, 73, 545, 958, 997, 1117, 1307, 1320
HpyCH4IV ACGT 1 cut(s) 754
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 498, 1102
HpyF3I CTNAG 3 cut(s) 267, 504, 1260
HpySE526I ACGT 1 cut(s) 754
HspAI GCGC 4 cut(s) 568, 658, 835, 837
Kzo9I GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
LmnI GCTCC 3 cut(s) 709, 820, 1219
Lsp1109I GCAGC 3 cut(s) 88, 476, 826
LweI GCATC 3 cut(s) 418, 726, 934
MaeI CTAG 2 cut(s) 425, 675
MaeII ACGT 1 cut(s) 754
MaeIII GTNAC 2 cut(s) 970, 1043
MalI GATC 6 cut(s) 249, 308, 645, 801, 864, 1256
MbiI CCGCTC 1 cut(s) 889
MboI GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
MboII GAAGA 6 cut(s) 242, 289, 316, 771, 843, 997
MflI RGATCY 2 cut(s) 247, 1254
MhlI GDGCHC 2 cut(s) 1192, 1252
MlsI TGGCCA 1 cut(s) 459
MluCI AATT 5 cut(s) 402, 444, 559, 795, 1067
MluNI TGGCCA 1 cut(s) 459
MmeI TCCRAC 2 cut(s) 41, 1012
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 1 cut(s) 949
MscI TGGCCA 1 cut(s) 459
MseI TTAA 6 cut(s) 215, 401, 443, 447, 591, 663
MslI CAYNNNNRTG 2 cut(s) 639, 829
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 2 cut(s) 101, 463
MspCI CTTAAG 1 cut(s) 214
MspI CCGG 1 cut(s) 740
MspR9I CCNGG 2 cut(s) 199, 586
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 2 cut(s) 199, 586
MvnI CGCG 1 cut(s) 837
MwoI GCNNNNNNNGC 3 cut(s) 142, 498, 1102
NcoI CCATGG 1 cut(s) 830
NdeI CATATG 1 cut(s) 949
NdeII GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
NlaIV GGNNCC 3 cut(s) 42, 822, 1215
NmuCI GTSAC 2 cut(s) 970, 1043
NsiI ATGCAT 1 cut(s) 949
NspI RCATGY 3 cut(s) 538, 1008, 1154
PacI TTAATTAA 1 cut(s) 447
PaqCI CACCTGC 1 cut(s) 1235
PasI CCCWGGG 1 cut(s) 198
PauI GCGCGC 1 cut(s) 835
PceI AGGCCT 1 cut(s) 453
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 2 cut(s) 694, 844
PflMI CCANNNNNTGG 1 cut(s) 244
PfoI TCCNGGA 1 cut(s) 584
PkrI GCNGC 3 cut(s) 103, 491, 841
Psp6I CCWGG 2 cut(s) 197, 584
PspGI CCWGG 2 cut(s) 197, 584
PspN4I GGNNCC 3 cut(s) 42, 822, 1215
PspPI GGNCC 3 cut(s) 551, 1034, 1169
PsuI RGATCY 2 cut(s) 247, 1254
PteI GCGCGC 1 cut(s) 835
PvuII CAGCTG 2 cut(s) 101, 463
RsaI GTAC 5 cut(s) 70, 261, 423, 618, 746
RsaNI GTAC 5 cut(s) 69, 260, 422, 617, 745
RseI CAYNNNNRTG 2 cut(s) 639, 829
SaqAI TTAA 6 cut(s) 215, 401, 443, 447, 591, 663
SatI GCNGC 3 cut(s) 102, 490, 840
Sau3AI GATC 6 cut(s) 247, 306, 643, 799, 862, 1254
Sau96I GGNCC 3 cut(s) 551, 1034, 1169
ScaI AGTACT 1 cut(s) 423
ScrFI CCNGG 2 cut(s) 199, 586
SduI GDGCHC 2 cut(s) 1192, 1252
SfaNI GCATC 3 cut(s) 418, 726, 934
SinI GGWCC 1 cut(s) 1034
SmiMI CAYNNNNRTG 2 cut(s) 639, 829
SmlI CTYRAG 2 cut(s) 214, 1193
SmoI CTYRAG 2 cut(s) 214, 1193
Sse9I AATT 5 cut(s) 402, 444, 559, 795, 1067
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 2 cut(s) 889, 1220
SspMI CTAG 2 cut(s) 425, 675
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 2 cut(s) 197, 584
StyI CCWWGG 3 cut(s) 454, 830, 1037
TaaI ACNGT 8 cut(s) 22, 73, 545, 958, 997, 1117, 1307, 1320
TaiI ACGT 1 cut(s) 757
TaqI TCGA 4 cut(s) 366, 624, 757, 867
TasI AATT 5 cut(s) 402, 444, 559, 795, 1067
TatI WGTACW 1 cut(s) 421
TfiI GAWTC 2 cut(s) 694, 844
Tru1I TTAA 6 cut(s) 215, 401, 443, 447, 591, 663
Tru9I TTAA 6 cut(s) 215, 401, 443, 447, 591, 663
TscAI CASTG 3 cut(s) 1000, 1312, 1323
TseFI GTSAC 2 cut(s) 970, 1043
TseI GCWGC 3 cut(s) 101, 489, 839
Tsp45I GTSAC 2 cut(s) 970, 1043
TspRI CASTG 3 cut(s) 1000, 1312, 1323
Van91I CCANNNNNTGG 1 cut(s) 244
Vha464I CTTAAG 1 cut(s) 214
VneI GTGCAC 2 cut(s) 1188, 1248
VpaK11BI GGWCC 1 cut(s) 1034
XapI RAATTY 1 cut(s) 1067
XceI RCATGY 3 cut(s) 538, 1008, 1154
XcmI CCANNNNNNNNNTGG 2 cut(s) 1085, 1143
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 2 cut(s) 425, 675
ZrmI AGTACT 1 cut(s) 423
Zsp2I ATGCAT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.