pycom05g22690

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0020141
Physical Location & Seq
Forward (+)
24921011 .. 24922239
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22690.2

Sequence Viewer

Length: 1098 bp
ATGTGGATCTATCAGGATGTGCCTGTACTACCTAAGAATAAGAAGAAACCGTATCCTATTCCCTTAAATAAGATCAAGCAGGCCGCAAAGGAGGACAAAAAACTTGCAGAATTGGGTATAGAGAAGCCCCTCGAACCTCCAAAAAATGGATTACTTGCCCCTGATCTGATTCCCGTTGCTCACCATGTACTAGATTCCTGGAAAATTTTAATTAAAGGCATTGCGCAGCTGTTGCACATCATTCCGGTATACGCTTGCACTGAATGCTCTGAGGTTCATGTGAGCCACTCTGGTCATCACATACAGGACTGTCTTGGTGCAACCAGTGCTAAACGCCGGAGCTTCCACTCCTGGATCAAGGGTTCCGTCAATGACGTACTTGTACCCATCGAGGCATATCATCTCTATGACCCTTTTGGTCGGCGAATTAAGCATGAAACTAGATTTCAATACGACAGGATTCCAGCTGTTGTAGAGCTGTGCATCCAAGCTGGTGTCGAGATCCCTGAGTACCCTTCACGTCGAAGAACCAAACCCATCCGTATGATTGGAAGGAAAGTGATCGATCGAGGTGGATTGATTGAGGAGCCTCTACCAGGGATCTCTGCAAATCCATCATCACTTATTGATCTCGATACACATGGGGCTTGTGGGCGGTTTCCACCTCCACCACCATCAGATATTCCCAAGATTGCACAGGAGACAATGGATGCATATGAAACTGTTAAGTTGGGGGTGACGAAGCTGATGGATAAATACACAGTGAAGGCATGCGGGTATTGCTCCGAGGTTCACGTGGGACCTTGGGGTCACAATGCGAAGCTTTGTGGGGAATTCAAGCACCAATGGAGGGATGGGAAGCATGGCTGGCAGGATGCTACCGTGGATGAACTTTTCCCTCCAAATTACGTATGGCATGTCGAAGACCCCAAAGGGCCTCCGTTGAAGGGCGGTGCACTCAAGAAGTTCTACGGGAAGGCTCCGGCAGTTGTAGAGGTTTGCTTGCAGGCAGGTGCACAGATCCCTGAGAAATATAAACCCATGATGAGGCTTGACATTGTTGTCCCTGAAAGTGAGGAGGCACAATTAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

366

Amino Acids

40.83

Weight (kDa)

8.57

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020141)

Species Orthologous Gene IDs
malus_domestica MD05G1249600.v1.1 MD10G1230000.v1.1
prunus_persica Prupe.4G114500_v2.0.a1 Prupe.4G114500_v2.0.a1
pyrus_communis pycom05g22690 pycom10g19360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1001
AasI GACNNNNNNGTC 2 cut(s) 807, 1061
Acc16I TGCGCA 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 146
AccI GTMKAC 1 cut(s) 249
AciI CCGC 4 cut(s) 84, 655, 774, 951
AclWI GGATC 5 cut(s) 14, 362, 496, 608, 1015
AcsI RAATTY 2 cut(s) 204, 833
AcvI CACGTG 1 cut(s) 796
AfaI GTAC 5 cut(s) 27, 189, 378, 384, 512
AfiI CCNNNNNNNGG 5 cut(s) 146, 596, 850, 947, 1047
AgsI TTSAA 3 cut(s) 449, 838, 946
AjiI CACGTC 1 cut(s) 521
AjnI CCWGG 3 cut(s) 197, 350, 595
AloI GAACNNNNNNTCC 2 cut(s) 346, 378
AluBI AGCT 7 cut(s) 229, 342, 467, 478, 491, 745, 823
AluI AGCT 7 cut(s) 229, 342, 467, 478, 491, 745, 823
Alw21I GWGCWC 2 cut(s) 958, 1018
Alw26I GTCTC 1 cut(s) 695
Alw44I GTGCAC 2 cut(s) 954, 1014
AlwI GGATC 5 cut(s) 14, 362, 496, 608, 1015
AoxI GGCC 2 cut(s) 81, 935
ApaLI GTGCAC 2 cut(s) 954, 1014
ApeKI GCWGC 1 cut(s) 226
ApoI RAATTY 2 cut(s) 204, 833
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 800, 935
AsuHPI GGTGA 2 cut(s) 173, 748
AvaII GGWCC 1 cut(s) 800
BaeGI GKGCMC 2 cut(s) 958, 1018
BbrPI CACGTG 1 cut(s) 796
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 2 cut(s) 958, 1018
BbvI GCAGC 1 cut(s) 238
BccI CCATC 6 cut(s) 395, 545, 622, 682, 742, 848
BciT130I CCWGG 3 cut(s) 199, 352, 597
BciVI GTATCC 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 2 cut(s) 191, 441
BfuAI ACCTGC 1 cut(s) 1001
BfuI GTATCC 1 cut(s) 63
BisI GCNGC 2 cut(s) 84, 227
BlsI GCNGC 2 cut(s) 85, 228
Bme1390I CCNGG 3 cut(s) 199, 352, 597
Bme18I GGWCC 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 521
BmgT120I GGNCC 2 cut(s) 800, 935
BmiI GGNNCC 4 cut(s) 364, 588, 801, 981
BmrFI CCNGG 3 cut(s) 199, 352, 597
BmsI GCATC 3 cut(s) 492, 700, 865
BpiI GAAGAC 1 cut(s) 930
BpuEI CTTGAG 1 cut(s) 944
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 2 cut(s) 796, 910
BsaJI CCNNGG 4 cut(s) 596, 786, 803, 882
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 596, 850, 947, 1047
Bse1I ACTGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 219
BseBI CCWGG 3 cut(s) 199, 352, 597
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 4 cut(s) 596, 786, 803, 882
BseGI GGATG 7 cut(s) 22, 483, 537, 715, 859, 880, 892
BseLI CCNNNNNNNGG 5 cut(s) 146, 596, 850, 947, 1047
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 3 cut(s) 261, 498, 1017
BseNI ACTGG 1 cut(s) 324
BseRI GAGGAG 2 cut(s) 599, 1091
BseSI GKGCMC 2 cut(s) 958, 1018
BseXI GCAGC 1 cut(s) 238
Bsh1285I CGRYCG 1 cut(s) 568
BshFI GGCC 2 cut(s) 83, 937
BshVI ATCGAT 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 568
BsiHKAI GWGCWC 2 cut(s) 958, 1018
BsiSI CCGG 3 cut(s) 245, 337, 983
BslFI GGGAC 2 cut(s) 813, 1049
BslI CCNNNNNNNGG 5 cut(s) 146, 596, 850, 947, 1047
BsmAI GTCTC 1 cut(s) 695
BsmFI GGGAC 2 cut(s) 813, 1049
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 2 cut(s) 83, 937
Bsp1286I GDGCHC 2 cut(s) 958, 1018
BspACI CCGC 4 cut(s) 84, 655, 774, 951
BspANI GGCC 2 cut(s) 83, 937
BspCNI CTCAG 3 cut(s) 262, 499, 1018
BspDI ATCGAT 1 cut(s) 564
BspLI GGNNCC 4 cut(s) 364, 588, 801, 981
BspMI ACCTGC 1 cut(s) 1001
BspPI GGATC 5 cut(s) 14, 362, 496, 608, 1015
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 1 cut(s) 324
BssECI CCNNGG 4 cut(s) 596, 786, 803, 882
BssNAI GTATAC 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 803
Bst1107I GTATAC 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 199, 352, 597
Bst4CI ACNGT 5 cut(s) 51, 311, 724, 763, 883
BstAPI GCANNNNNTGC 3 cut(s) 232, 264, 326
BstBAI YACGTR 2 cut(s) 796, 910
BstC8I GCNNGC 6 cut(s) 81, 256, 772, 869, 1004, 1008
BstDEI CTNAG 4 cut(s) 33, 270, 507, 1026
BstDSI CCRYGG 1 cut(s) 882
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 7 cut(s) 22, 483, 537, 715, 859, 880, 892
BstHHI GCGC 1 cut(s) 226
BstMAI GTCTC 1 cut(s) 695
BstMCI CGRYCG 1 cut(s) 568
BstMWI GCNNNNNNNGC 6 cut(s) 232, 264, 326, 430, 780, 868
BstNI CCWGG 3 cut(s) 199, 352, 597
BstNSI RCATGY 2 cut(s) 774, 920
BstSCI CCNGG 3 cut(s) 197, 350, 595
BstSLI GKGCMC 2 cut(s) 958, 1018
BstSNI TACGTA 1 cut(s) 910
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 930
BstX2I RGATCY 4 cut(s) 6, 501, 600, 1020
BstYI RGATCY 4 cut(s) 6, 501, 600, 1020
BstZ17I GTATAC 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 564
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 2 cut(s) 83, 937
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 882
BtrI CACGTC 1 cut(s) 521
BtsCI GGATG 7 cut(s) 22, 483, 537, 715, 859, 880, 892
BtsIMutI CAGTG 3 cut(s) 258, 331, 768
BveI ACCTGC 1 cut(s) 1001
Cac8I GCNNGC 6 cut(s) 81, 256, 772, 869, 1004, 1008
CfoI GCGC 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 800, 935
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 5 cut(s) 26, 188, 377, 383, 511
CviAII CATG 8 cut(s) 185, 278, 434, 641, 771, 863, 917, 1042
CviQI GTAC 5 cut(s) 26, 188, 377, 383, 511
DdeI CTNAG 4 cut(s) 33, 270, 507, 1026
DrdI GACNNNNNNGTC 2 cut(s) 807, 1061
DseDI GACNNNNNNGTC 2 cut(s) 807, 1061
Eco105I TACGTA 1 cut(s) 910
Eco130I CCWWGG 1 cut(s) 803
Eco47I GGWCC 1 cut(s) 800
Eco72I CACGTG 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 2 cut(s) 800, 935
EcoRI GAATTC 1 cut(s) 833
EcoRII CCWGG 3 cut(s) 197, 350, 595
EcoT14I CCWWGG 1 cut(s) 803
EcoT22I ATGCAT 1 cut(s) 715
ErhI CCWWGG 1 cut(s) 803
FaeI CATG 8 cut(s) 188, 281, 437, 644, 774, 866, 920, 1045
FaqI GGGAC 2 cut(s) 813, 1049
FatI CATG 8 cut(s) 184, 277, 433, 640, 770, 862, 916, 1041
FauI CCCGC 1 cut(s) 767
FauNDI CATATG 1 cut(s) 715
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 84, 227
FokI GGATG 7 cut(s) 29, 470, 524, 722, 866, 887, 899
Fsp4HI GCNGC 2 cut(s) 84, 227
FspBI CTAG 2 cut(s) 191, 441
FspI TGCGCA 1 cut(s) 225
GlaI GCGC 1 cut(s) 225
GluI GCNGC 2 cut(s) 84, 227
HaeIII GGCC 2 cut(s) 83, 937
HapII CCGG 3 cut(s) 245, 337, 983
HhaI GCGC 1 cut(s) 226
Hin1II CATG 8 cut(s) 188, 281, 437, 644, 774, 866, 920, 1045
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 3 cut(s) 169, 194, 460
HpaII CCGG 3 cut(s) 245, 337, 983
HphI GGTGA 2 cut(s) 173, 748
Hpy166II GTNNAC 4 cut(s) 250, 793, 956, 1016
Hpy188I TCNGA 4 cut(s) 168, 271, 679, 787
Hpy188III TCNNGA 4 cut(s) 14, 499, 632, 961
Hpy8I GTNNAC 4 cut(s) 250, 793, 956, 1016
Hpy99I CGWCG 1 cut(s) 525
HpyAV CCTTC 5 cut(s) 525, 546, 760, 940, 970
HpyCH4III ACNGT 5 cut(s) 51, 311, 724, 763, 883
HpyCH4IV ACGT 4 cut(s) 375, 520, 795, 909
HpyF10VI GCNNNNNNNGC 6 cut(s) 232, 264, 326, 430, 780, 868
HpyF3I CTNAG 4 cut(s) 33, 270, 507, 1026
HpySE526I ACGT 4 cut(s) 375, 520, 795, 909
Hsp92II CATG 8 cut(s) 188, 281, 437, 644, 774, 866, 920, 1045
HspAI GCGC 1 cut(s) 224
LmnI GCTCC 4 cut(s) 339, 586, 788, 985
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 3 cut(s) 492, 700, 865
MaeI CTAG 2 cut(s) 191, 441
MaeII ACGT 4 cut(s) 375, 520, 795, 909
MaeIII GTNAC 2 cut(s) 736, 809
MboII GAAGA 3 cut(s) 55, 537, 935
MflI RGATCY 4 cut(s) 6, 501, 600, 1020
MhlI GDGCHC 2 cut(s) 958, 1018
MluCI AATT 7 cut(s) 110, 204, 210, 426, 833, 904, 1085
Mph1103I ATGCAT 1 cut(s) 715
MseI TTAA 5 cut(s) 65, 209, 213, 429, 726
MslI CAYNNNNRTG 2 cut(s) 405, 542
MspA1I CMGCKG 2 cut(s) 229, 467
MspI CCGG 3 cut(s) 245, 337, 983
MspR9I CCNGG 3 cut(s) 199, 352, 597
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 3 cut(s) 199, 352, 597
MwoI GCNNNNNNNGC 6 cut(s) 232, 264, 326, 430, 780, 868
NdeI CATATG 1 cut(s) 715
NlaIII CATG 8 cut(s) 188, 281, 437, 644, 774, 866, 920, 1045
NlaIV GGNNCC 4 cut(s) 364, 588, 801, 981
NmuCI GTSAC 2 cut(s) 736, 809
NsbI TGCGCA 1 cut(s) 225
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 2 cut(s) 774, 920
PacI TTAATTAA 1 cut(s) 213
PaeI GCATGC 1 cut(s) 774
PaqCI CACCTGC 1 cut(s) 1001
PctI GAATGC 1 cut(s) 269
PfeI GAWTC 3 cut(s) 169, 194, 460
PflMI CCANNNNNTGG 1 cut(s) 146
PfoI TCCNGGA 2 cut(s) 197, 350
PkrI GCNGC 2 cut(s) 85, 228
Ple19I CGATCG 1 cut(s) 568
PmaCI CACGTG 1 cut(s) 796
PmlI CACGTG 1 cut(s) 796
Ppu21I YACGTR 2 cut(s) 796, 910
PpuMI RGGWCCY 1 cut(s) 800
Psp5II RGGWCCY 1 cut(s) 800
Psp6I CCWGG 3 cut(s) 197, 350, 595
PspCI CACGTG 1 cut(s) 796
PspGI CCWGG 3 cut(s) 197, 350, 595
PspN4I GGNNCC 4 cut(s) 364, 588, 801, 981
PspPI GGNCC 2 cut(s) 800, 935
PspPPI RGGWCCY 1 cut(s) 800
PsuI RGATCY 4 cut(s) 6, 501, 600, 1020
PvuI CGATCG 1 cut(s) 568
PvuII CAGCTG 2 cut(s) 229, 467
RsaI GTAC 5 cut(s) 27, 189, 378, 384, 512
RsaNI GTAC 5 cut(s) 26, 188, 377, 383, 511
RseI CAYNNNNRTG 2 cut(s) 405, 542
SaqAI TTAA 5 cut(s) 65, 209, 213, 429, 726
SatI GCNGC 2 cut(s) 84, 227
Sau96I GGNCC 2 cut(s) 800, 935
ScrFI CCNGG 3 cut(s) 199, 352, 597
SduI GDGCHC 2 cut(s) 958, 1018
SfaNI GCATC 3 cut(s) 492, 700, 865
SinI GGWCC 1 cut(s) 800
SmiMI CAYNNNNRTG 2 cut(s) 405, 542
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
SnaBI TACGTA 1 cut(s) 910
SphI GCATGC 1 cut(s) 774
Sse9I AATT 7 cut(s) 110, 204, 210, 426, 833, 904, 1085
SsiI CCGC 4 cut(s) 84, 655, 774, 951
SspMI CTAG 2 cut(s) 191, 441
StyD4I CCNGG 3 cut(s) 197, 350, 595
StyI CCWWGG 1 cut(s) 803
TaaI ACNGT 5 cut(s) 51, 311, 724, 763, 883
TaiI ACGT 4 cut(s) 378, 523, 798, 912
TaqI TCGA 8 cut(s) 132, 390, 498, 523, 564, 568, 633, 921
TasI AATT 7 cut(s) 110, 204, 210, 426, 833, 904, 1085
TatI WGTACW 2 cut(s) 25, 187
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 3 cut(s) 169, 194, 460
Tru1I TTAA 5 cut(s) 65, 209, 213, 429, 726
Tru9I TTAA 5 cut(s) 65, 209, 213, 429, 726
TscAI CASTG 3 cut(s) 265, 331, 768
TseFI GTSAC 2 cut(s) 736, 809
TseI GCWGC 1 cut(s) 226
Tsp45I GTSAC 2 cut(s) 736, 809
TspDTI ATGAA 4 cut(s) 266, 450, 732, 903
TspGWI ACGGA 3 cut(s) 355, 530, 930
TspRI CASTG 3 cut(s) 265, 331, 768
Van91I CCANNNNNTGG 1 cut(s) 146
VneI GTGCAC 2 cut(s) 954, 1014
VpaK11BI GGWCC 1 cut(s) 800
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 2 cut(s) 204, 833
XceI RCATGY 2 cut(s) 774, 920
XcmI CCANNNNNNNNNTGG 2 cut(s) 851, 909
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 2 cut(s) 191, 441
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.