Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013431

Physical Location & Seq

Genomic Coordinates Forward (+)

240843 .. 246501

Gene Structure 5659 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1002300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGATTCCGACTACGGCATTCCCAGAGAGCTCTCTGATCTTCAGAAACTCCGATCCCAATACCAACCCGAGCTTCCTCCTTGCCTCCAGGGAGCTACCGTGAGGGTTGAATTTGGTGATACAACCACATCTTTAGATCCCACCGATTCCCACACCATCAGTCGCTACTTTCCCCACACTTACGGTCAGCCTTTGGCTCACTTTCTCAGGGCAACTGCCAAAGTCCCTGGTGCTCAGGTTATTACTGAGCATCCCCCTGTTAGGGTGGGAGTTGTCTTTTGTGGGAGACAATCACCTGGAGGACATAATGTTATATGGGGTCTTCACAACGCTCTAAAAGTTCACAACCCTAACAATACCTTACTTGGGTTTTTGGGGGGTTCTGAAGGTTTGTTTGCACAAAAAACTCTTGAGATAACAGATGAAATTCTTGCAACCTACAAAAACCAAGGTGGTTATGATTTGCTGGGAAGGACAAAAGACCAAATTAGAACAGTTGAGCAAGTCAACGCTACACTTACCGCATGCAAGGATTTGAAATTGGATGGCCTTGTCATTATTGGAGGTGTGACCTCAAATACCGATGCCGCCCAGCTTGCAGAGACATTTGCGGAAGCAAAATGCTCAACAAAGGTGGTTGGTGTTCCTGTCACTTTGAATGGAGATCTTAAGAACCAGTTTGTCGAAGCAAATGTTGGTTTTGATACAATATGTAAGGTCAATTCCCAATTAATCAGCAATATGTGCACTGATGCTCTTTCGGCAGAGAAGTATTATTATTTCATTCGACTCATGGGCCGGAAGGCATCACATGTTGCGTTGGAGTGCACCCTCCAGTCCCATCCAAATTTGGTTATTCTTGGTGAGGAGGTGGCGGCATCAAAGCTAACTCTTTTTGACATTACAACACAAATTTGTGATGCAGTGGAAGCCCGTGCAGCACAAGACAAGAATCATGGTGTCATCCTATTACCTGAAGGACTCATAGAAAGCATTCCTGAGGTGTATGCCCTCTTGAAGGAAATTCATGGTTTGCACAGGCAAGGAGTATCTGCCGATAAAATTTCTACTCAACTTTCACCTTGGGCATCTGCATTGTTTGAATTCTTGCCACCATTTATCAAGAAACAGGTACTACTTTTGCCTGAATCAGATGACTCTGCGCAACTATCCCAGATTGAGACAGAGAAACTTCTTGCACATCTTGTGGAGGGGGAAATGAATAAGCGTCAGAAAGAGGGCACATACAAGGGGAAGAAATTTAATGCTATATGCCACTTCTTCGGTTATCAGGCTCGGGGGTCTTTACCATCAAAGTTCGATTGTGACTATGCTTATGTTCTAGGGCACATTGGCTACCATATTCTCGCAGCTGGTTTGAATGGTTACATGGCAACTATAACTAACTTGAGGAATCCTGTGAATAAGTGGCGTTGTGGTGCTGCTCCACTTACTGCAATGATGACTGTTAAGCGTTGGGCTCAAAATCCTGGGACTGCTTCAATTGGAAAACCAGCTATTCATCCCGCTACTGTAGATTTGAAAGGCAAAGCATATGACTTGTTGAGGCAAAATGCTACAAAATTCCTGCTTGATGATATCTACAGAAACCCAGGGCCTCTGCAGTTTGATGGTCCTGGTGCCGATTCTAAGGCTGTATCACTATGTGTTGAAGACCAGGATTACATGGGGCGTATCAAGAAACTGCAGGAGTATCTTGACAAGATTCGAACCATTGTAAAGCCTGGGTGCTCGCAAGAGGTTCTGAAAGCAGCTTTGAGTGTGATGGCGTCTGTGACAGAAGTCCTCTCGGTTATGTCCTCATCACCTATCAATGGCCAATCGACCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008443 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010033 GO:0015979 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0034284 GO:0034285 GO:0042221 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0047334 GO:0050896 GO:0071704 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko01100 ko01110 ko01120 ko01130 map00010 map00030 map00051 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

PFK

Protein Analysis

618

Amino Acids

67.42

Weight (kDa)

6.79

Isoelectric Point (pI)

41.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	88 - 348	6.8e-26	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20950 AT1G76550
fragaria_vesca	FvH4_5g26380 FvH4_5g26380
malus_domestica	MD06G1002300.v1.1
prunus_persica	Prupe.5G003400_v2.0.a1
pyrus_communis	pycom06g00240
rosa_chinensis	RchiOBHm_Chr7g0217221
rosa_laevigata	RLG00000002501
rosa_multiflora	Rmu_sc0007586.1_g000002 Rmu_sc0022408.1_g000001
rosa_roxburghii	Rroxscaffold_3G00242490
rosa_rugosa	Rorug07G0167300
rosa_samantha	Rh7AG306300 Rh7BG297600 Rh7CG325400 Rh7DG306100
rosa_wichuraiana	Rw7G026020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1164
AccB1I	GGYRCC	1 cut(s)	1640
AciI	CCGC	5 cut(s)	522, 588, 611, 875, 1527
AclWI	GGATC	2 cut(s)	48, 131
AcoI	YGGCCR	1 cut(s)	1837
AcsI	RAATTY	9 cut(s)	110, 426, 847, 912, 1023, 1062, 1103, 1259, 1583
AcuI	CTGAAG	3 cut(s)	26, 405, 996
AcyI	GRCGYC	1 cut(s)	1790
AdeI	CACNNNGTG	1 cut(s)	1667
AfaI	GTAC	1 cut(s)	1134
AfiI	CCNNNNNNNGG	5 cut(s)	261, 262, 366, 1018, 1835
AflII	CTTAAG	1 cut(s)	668
AflIII	ACRYGT	1 cut(s)	811
AgsI	TTSAA	9 cut(s)	110, 538, 658, 1018, 1103, 1381, 1503, 1543, 1673
AjnI	CCWGG	8 cut(s)	87, 226, 295, 1489, 1612, 1636, 1677, 1744
AjuI	GAANNNNNNNTTGG	4 cut(s)	57, 89, 678, 710
AluBI	AGCT	8 cut(s)	31, 73, 95, 595, 886, 1373, 1517, 1775
AluI	AGCT	8 cut(s)	31, 73, 95, 595, 886, 1373, 1517, 1775
Alw21I	GWGCWC	5 cut(s)	33, 235, 749, 830, 1754
Alw26I	GTCTC	3 cut(s)	280, 596, 1175
Alw44I	GTGCAC	2 cut(s)	745, 826
AlwI	GGATC	2 cut(s)	48, 131
Ama87I	CYCGRG	2 cut(s)	68, 1296
AoxI	GGCC	4 cut(s)	547, 796, 1616, 1837
ApaLI	GTGCAC	2 cut(s)	745, 826
ApeKI	GCWGC	4 cut(s)	938, 1370, 1442, 1772
ApoI	RAATTY	9 cut(s)	110, 426, 847, 912, 1023, 1062, 1103, 1259, 1583
AseI	ATTAAT	1 cut(s)	731
Asp700I	GAANNNNTTC	1 cut(s)	993
AspLEI	GCGC	1 cut(s)	1165
AspS9I	GGNCC	3 cut(s)	796, 1616, 1634
AsuHPI	GGTGA	5 cut(s)	128, 285, 875, 1071, 1818
AsuII	TTCGAA	1 cut(s)	1729
AvaI	CYCGRG	2 cut(s)	68, 1296
AvaII	GGWCC	1 cut(s)	1634
AxyI	CCTNAGG	1 cut(s)	999
BaeGI	GKGCMC	4 cut(s)	749, 830, 1244, 1350
BalI	TGGCCA	1 cut(s)	1839
BanI	GGYRCC	1 cut(s)	1640
BanII	GRGCYC	2 cut(s)	33, 1483
BbsI	GAAGAC	2 cut(s)	314, 1680
Bbv12I	GWGCWC	5 cut(s)	33, 235, 749, 830, 1754
BbvI	GCAGC	4 cut(s)	950, 1382, 1429, 1784
BccI	CCATC	6 cut(s)	164, 539, 849, 1318, 1625, 1780
BceAI	ACGGC	1 cut(s)	31
BciT130I	CCWGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
BcoDI	GTCTC	3 cut(s)	280, 596, 1175
BfaI	CTAG	1 cut(s)	1343
BfmI	CTRYAG	4 cut(s)	1533, 1603, 1622, 1706
BfrI	CTTAAG	1 cut(s)	668
BglII	AGATCT	1 cut(s)	664
BisI	GCNGC	6 cut(s)	588, 876, 939, 1371, 1443, 1773
BlsI	GCNGC	6 cut(s)	589, 877, 940, 1372, 1444, 1774
Bme1390I	CCNGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
Bme18I	GGWCC	1 cut(s)	1634
BmeT110I	CYCGRG	2 cut(s)	68, 1296
BmgT120I	GGNCC	3 cut(s)	796, 1616, 1634
BmiI	GGNNCC	1 cut(s)	1642
BmrFI	CCNGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
BmsI	GCATC	7 cut(s)	259, 574, 742, 815, 887, 910, 1097
BoxI	GACNNNNGTC	1 cut(s)	1802
BpiI	GAAGAC	2 cut(s)	314, 1680
BpmI	CTGGAG	3 cut(s)	71, 318, 818
Bpu10I	CCTNAGC	1 cut(s)	234
Bpu14I	TTCGAA	1 cut(s)	1729
BpuEI	CTTGAG	2 cut(s)	431, 1429
BsaHI	GRCGYC	1 cut(s)	1790
BsaJI	CCNNGG	8 cut(s)	88, 226, 448, 1082, 1490, 1612, 1613, 1745
BsaXI	ACNNNNNCTCC	2 cut(s)	84, 114
Bsc4I	CCNNNNNNNGG	5 cut(s)	261, 262, 366, 1018, 1835
Bse1I	ACTGG	2 cut(s)	676, 835
Bse21I	CCTNAGG	1 cut(s)	999
Bse3DI	GCAATG	1 cut(s)	1464
BseBI	CCWGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
BseDI	CCNNGG	8 cut(s)	88, 226, 448, 1082, 1490, 1612, 1613, 1745
BseGI	GGATG	5 cut(s)	250, 550, 841, 963, 1522
BseLI	CCNNNNNNNGG	5 cut(s)	261, 262, 366, 1018, 1835
BseMI	GCAATG	1 cut(s)	1464
BseMII	CTCAG	4 cut(s)	220, 237, 248, 990
BseNI	ACTGG	2 cut(s)	676, 835
BseRI	GAGGAG	1 cut(s)	881
BseSI	GKGCMC	4 cut(s)	749, 830, 1244, 1350
BseXI	GCAGC	4 cut(s)	950, 1382, 1429, 1784
BseYI	CCCAGC	2 cut(s)	466, 591
BsgI	GTGCAG	1 cut(s)	957
BshFI	GGCC	4 cut(s)	549, 798, 1618, 1839
BshNI	GGYRCC	1 cut(s)	1640
BsiHKAI	GWGCWC	5 cut(s)	33, 235, 749, 830, 1754
BsiHKCI	CYCGRG	2 cut(s)	68, 1296
BsiSI	CCGG	1 cut(s)	799
BslFI	GGGAC	3 cut(s)	209, 823, 1507
BslI	CCNNNNNNNGG	5 cut(s)	261, 262, 366, 1018, 1835
BsmAI	GTCTC	3 cut(s)	280, 596, 1175
BsmFI	GGGAC	3 cut(s)	209, 823, 1507
BsmI	GAATGC	2 cut(s)	18, 993
BsnI	GGCC	4 cut(s)	549, 798, 1618, 1839
BsoBI	CYCGRG	2 cut(s)	68, 1296
Bsp119I	TTCGAA	1 cut(s)	1729
Bsp1286I	GDGCHC	8 cut(s)	33, 235, 749, 830, 1244, 1350, 1483, 1754
Bsp143I	GATC	4 cut(s)	37, 53, 136, 664
BspACI	CCGC	5 cut(s)	522, 588, 611, 875, 1527
BspANI	GGCC	4 cut(s)	549, 798, 1618, 1839
BspCNI	CTCAG	4 cut(s)	219, 238, 247, 991
BspLI	GGNNCC	1 cut(s)	1642
BspMAI	CTGCAG	2 cut(s)	1626, 1710
BspPI	GGATC	2 cut(s)	48, 131
BspT104I	TTCGAA	1 cut(s)	1729
BspT107I	GGYRCC	1 cut(s)	1640
BspTI	CTTAAG	1 cut(s)	668
BsrDI	GCAATG	1 cut(s)	1464
BsrI	ACTGG	2 cut(s)	676, 835
BssECI	CCNNGG	8 cut(s)	88, 226, 448, 1082, 1490, 1612, 1613, 1745
BssMI	GATC	4 cut(s)	37, 53, 136, 664
BssNI	GRCGYC	1 cut(s)	1790
BssT1I	CCWWGG	2 cut(s)	448, 1082
Bst2UI	CCWGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
Bst4CI	ACNGT	5 cut(s)	100, 185, 496, 1468, 1534
BstACI	GRCGYC	1 cut(s)	1790
BstAFI	CTTAAG	1 cut(s)	668
BstAPI	GCANNNNNTGC	1 cut(s)	744
BstBI	TTCGAA	1 cut(s)	1729
BstC8I	GCNNGC	3 cut(s)	526, 597, 1754
BstDEI	CTNAG	5 cut(s)	206, 234, 246, 999, 1650
BstENI	CCTNNNNNAGG	1 cut(s)	1016
BstF5I	GGATG	5 cut(s)	250, 550, 841, 963, 1522
BstHHI	GCGC	1 cut(s)	1165
BstKTI	GATC	4 cut(s)	40, 56, 139, 667
BstMAI	GTCTC	3 cut(s)	280, 596, 1175
BstMBI	GATC	4 cut(s)	37, 53, 136, 664
BstMWI	GCNNNNNNNGC	5 cut(s)	596, 744, 761, 929, 938
BstNI	CCWGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
BstNSI	RCATGY	2 cut(s)	528, 815
BstPAI	GACNNNNGTC	1 cut(s)	1802
BstSCI	CCNGG	8 cut(s)	87, 226, 295, 1489, 1612, 1636, 1677, 1744
BstSFI	CTRYAG	4 cut(s)	1533, 1603, 1622, 1706
BstSLI	GKGCMC	4 cut(s)	749, 830, 1244, 1350
BstV1I	GCAGC	4 cut(s)	950, 1382, 1429, 1784
BstV2I	GAAGAC	2 cut(s)	314, 1680
BstX2I	RGATCY	2 cut(s)	136, 664
BstYI	RGATCY	2 cut(s)	136, 664
Bsu36I	CCTNAGG	1 cut(s)	999
BsuRI	GGCC	4 cut(s)	549, 798, 1618, 1839
BtsCI	GGATG	5 cut(s)	250, 550, 841, 963, 1522
BtsI	GCAGTG	1 cut(s)	930
BtsIMutI	CAGTG	2 cut(s)	747, 930
Cac8I	GCNNGC	3 cut(s)	526, 597, 1754
CfoI	GCGC	1 cut(s)	1165
Cfr13I	GGNCC	3 cut(s)	796, 1616, 1634
CseI	GACGC	2 cut(s)	1217, 1779
Csp6I	GTAC	1 cut(s)	1133
CspCI	CAANNNNNGTGG	2 cut(s)	615, 650
CviAII	CATG	7 cut(s)	525, 793, 812, 956, 1028, 1390, 1687
CviQI	GTAC	1 cut(s)	1133
DdeI	CTNAG	5 cut(s)	206, 234, 246, 999, 1650
DpnI	GATC	4 cut(s)	39, 55, 138, 666
DpnII	GATC	4 cut(s)	37, 53, 136, 664
DraIII	CACNNNGTG	1 cut(s)	1667
EaeI	YGGCCR	1 cut(s)	1837
Ecl136II	GAGCTC	1 cut(s)	31
Eco130I	CCWWGG	2 cut(s)	448, 1082
Eco24I	GRGCYC	2 cut(s)	33, 1483
Eco32I	GATATC	1 cut(s)	1600
Eco47I	GGWCC	1 cut(s)	1634
Eco53kI	GAGCTC	1 cut(s)	31
Eco57I	CTGAAG	3 cut(s)	26, 405, 996
Eco81I	CCTNAGG	1 cut(s)	999
Eco88I	CYCGRG	2 cut(s)	68, 1296
EcoICRI	GAGCTC	1 cut(s)	31
EcoNI	CCTNNNNNAGG	1 cut(s)	1016
EcoO109I	RGGNCCY	1 cut(s)	1616
EcoRI	GAATTC	1 cut(s)	1103
EcoRII	CCWGG	8 cut(s)	87, 226, 295, 1489, 1612, 1636, 1677, 1744
EcoRV	GATATC	1 cut(s)	1600
EcoT14I	CCWWGG	2 cut(s)	448, 1082
EcoT38I	GRGCYC	2 cut(s)	33, 1483
ErhI	CCWWGG	2 cut(s)	448, 1082
FaeI	CATG	7 cut(s)	528, 796, 815, 959, 1031, 1393, 1690
FaqI	GGGAC	3 cut(s)	209, 823, 1507
FatI	CATG	7 cut(s)	524, 792, 811, 955, 1027, 1389, 1686
FauI	CCCGC	1 cut(s)	1534
FauNDI	CATATG	1 cut(s)	1555
Fnu4HI	GCNGC	6 cut(s)	588, 876, 939, 1371, 1443, 1773
FokI	GGATG	5 cut(s)	237, 557, 828, 950, 1509
FriOI	GRGCYC	2 cut(s)	33, 1483
Fsp4HI	GCNGC	6 cut(s)	588, 876, 939, 1371, 1443, 1773
FspBI	CTAG	1 cut(s)	1343
FspI	TGCGCA	1 cut(s)	1164
GlaI	GCGC	1 cut(s)	1164
GluI	GCNGC	6 cut(s)	588, 876, 939, 1371, 1443, 1773
GsaI	CCCAGC	2 cut(s)	470, 595
GsuI	CTGGAG	3 cut(s)	71, 318, 818
HaeIII	GGCC	4 cut(s)	549, 798, 1618, 1839
HapII	CCGG	1 cut(s)	799
HgaI	GACGC	2 cut(s)	1217, 1779
HhaI	GCGC	1 cut(s)	1165
Hin1I	GRCGYC	1 cut(s)	1790
Hin1II	CATG	7 cut(s)	528, 796, 815, 959, 1031, 1393, 1690
Hin6I	GCGC	1 cut(s)	1163
HinP1I	GCGC	1 cut(s)	1163
HincII	GTYRAC	1 cut(s)	508
HindII	GTYRAC	1 cut(s)	508
HpaII	CCGG	1 cut(s)	799
HphI	GGTGA	5 cut(s)	128, 285, 875, 1071, 1818
Hpy166II	GTNNAC	4 cut(s)	343, 508, 747, 828
Hpy188I	TCNGA	8 cut(s)	10, 37, 45, 53, 385, 1153, 1233, 1767
Hpy188III	TCNNGA	6 cut(s)	410, 998, 1015, 1123, 1699, 1718
Hpy8I	GTNNAC	4 cut(s)	343, 508, 747, 828
HpyAV	CCTTC	5 cut(s)	380, 465, 796, 971, 1012
HpyCH4III	ACNGT	5 cut(s)	100, 185, 496, 1468, 1534
HpyF10VI	GCNNNNNNNGC	5 cut(s)	596, 744, 761, 929, 938
HpyF3I	CTNAG	5 cut(s)	206, 234, 246, 999, 1650
Hsp92I	GRCGYC	1 cut(s)	1790
Hsp92II	CATG	7 cut(s)	528, 796, 815, 959, 1031, 1393, 1690
HspAI	GCGC	1 cut(s)	1163
Kzo9I	GATC	4 cut(s)	37, 53, 136, 664
LmnI	GCTCC	2 cut(s)	92, 1450
Lsp1109I	GCAGC	4 cut(s)	950, 1382, 1429, 1784
LweI	GCATC	7 cut(s)	259, 574, 742, 815, 887, 910, 1097
MaeI	CTAG	1 cut(s)	1343
MaeIII	GTNAC	5 cut(s)	568, 649, 1325, 1385, 1795
MalI	GATC	4 cut(s)	39, 55, 138, 666
MboI	GATC	4 cut(s)	37, 53, 136, 664
MboII	GAAGA	5 cut(s)	32, 314, 1267, 1273, 1685
MfeI	CAATTG	1 cut(s)	1503
MflI	RGATCY	2 cut(s)	136, 664
MhlI	GDGCHC	8 cut(s)	33, 235, 749, 830, 1244, 1350, 1483, 1754
MlsI	TGGCCA	1 cut(s)	1839
MluNI	TGGCCA	1 cut(s)	1839
MlyI	GAGTC	3 cut(s)	783, 975, 1151
MmeI	TCCRAC	2 cut(s)	33, 801
Mox20I	TGGCCA	1 cut(s)	1839
MroXI	GAANNNNTTC	1 cut(s)	993
MscI	TGGCCA	1 cut(s)	1839
MseI	TTAA	5 cut(s)	669, 731, 1263, 1470, 1852
Msp20I	TGGCCA	1 cut(s)	1839
MspA1I	CMGCKG	1 cut(s)	1373
MspCI	CTTAAG	1 cut(s)	668
MspI	CCGG	1 cut(s)	799
MspR9I	CCNGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
MunI	CAATTG	1 cut(s)	1503
Mva1269I	GAATGC	2 cut(s)	18, 993
MvaI	CCWGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
MwoI	GCNNNNNNNGC	5 cut(s)	596, 744, 761, 929, 938
NdeI	CATATG	1 cut(s)	1555
NdeII	GATC	4 cut(s)	37, 53, 136, 664
NlaIII	CATG	7 cut(s)	528, 796, 815, 959, 1031, 1393, 1690
NlaIV	GGNNCC	1 cut(s)	1642
NmuCI	GTSAC	4 cut(s)	568, 649, 1325, 1795
NsbI	TGCGCA	1 cut(s)	1164
NspI	RCATGY	2 cut(s)	528, 815
NspV	TTCGAA	1 cut(s)	1729
PaeI	GCATGC	1 cut(s)	528
PasI	CCCWGGG	1 cut(s)	1613
PciI	ACATGT	1 cut(s)	811
PctI	GAATGC	2 cut(s)	18, 993
PdmI	GAANNNNTTC	1 cut(s)	993
PfeI	GAWTC	7 cut(s)	5, 146, 952, 1148, 1414, 1646, 1726
PkrI	GCNGC	6 cut(s)	589, 877, 940, 1372, 1444, 1774
PleI	GAGTC	3 cut(s)	783, 975, 1151
PpsI	GAGTC	3 cut(s)	783, 975, 1151
PscI	ACATGT	1 cut(s)	811
PshAI	GACNNNNGTC	1 cut(s)	1802
PshBI	ATTAAT	1 cut(s)	731
Psp124BI	GAGCTC	1 cut(s)	33
Psp6I	CCWGG	8 cut(s)	87, 226, 295, 1489, 1612, 1636, 1677, 1744
PspFI	CCCAGC	2 cut(s)	466, 591
PspGI	CCWGG	8 cut(s)	87, 226, 295, 1489, 1612, 1636, 1677, 1744
PspN4I	GGNNCC	1 cut(s)	1642
PspPI	GGNCC	3 cut(s)	796, 1616, 1634
PstI	CTGCAG	2 cut(s)	1626, 1710
PsuI	RGATCY	2 cut(s)	136, 664
PvuII	CAGCTG	1 cut(s)	1373
RsaI	GTAC	1 cut(s)	1134
RsaNI	GTAC	1 cut(s)	1133
SacI	GAGCTC	1 cut(s)	33
SaqAI	TTAA	5 cut(s)	669, 731, 1263, 1470, 1852
SatI	GCNGC	6 cut(s)	588, 876, 939, 1371, 1443, 1773
Sau3AI	GATC	4 cut(s)	37, 53, 136, 664
Sau96I	GGNCC	3 cut(s)	796, 1616, 1634
SchI	GAGTC	3 cut(s)	783, 975, 1151
ScrFI	CCNGG	8 cut(s)	89, 228, 297, 1491, 1614, 1638, 1679, 1746
SduI	GDGCHC	8 cut(s)	33, 235, 749, 830, 1244, 1350, 1483, 1754
SfaNI	GCATC	7 cut(s)	259, 574, 742, 815, 887, 910, 1097
SfcI	CTRYAG	4 cut(s)	1533, 1603, 1622, 1706
SfuI	TTCGAA	1 cut(s)	1729
SinI	GGWCC	1 cut(s)	1634
SmlI	CTYRAG	3 cut(s)	410, 668, 1408
SmoI	CTYRAG	3 cut(s)	410, 668, 1408
SphI	GCATGC	1 cut(s)	528
SsiI	CCGC	5 cut(s)	522, 588, 611, 875, 1527
SspMI	CTAG	1 cut(s)	1343
SstI	GAGCTC	1 cut(s)	33
StyD4I	CCNGG	8 cut(s)	87, 226, 295, 1489, 1612, 1636, 1677, 1744
StyI	CCWWGG	2 cut(s)	448, 1082
TaaI	ACNGT	5 cut(s)	100, 185, 496, 1468, 1534
TaqI	TCGA	5 cut(s)	684, 787, 1320, 1729, 1844
TauI	GCSGC	2 cut(s)	590, 878
TfiI	GAWTC	7 cut(s)	5, 146, 952, 1148, 1414, 1646, 1726
Tru1I	TTAA	5 cut(s)	669, 731, 1263, 1470, 1852
Tru9I	TTAA	5 cut(s)	669, 731, 1263, 1470, 1852
TscAI	CASTG	2 cut(s)	754, 930
TseFI	GTSAC	4 cut(s)	568, 649, 1325, 1795
TseI	GCWGC	4 cut(s)	938, 1370, 1442, 1772
Tsp45I	GTSAC	4 cut(s)	568, 649, 1325, 1795
TspDTI	ATGAA	5 cut(s)	438, 772, 1016, 1235, 1511
TspRI	CASTG	2 cut(s)	754, 930
Vha464I	CTTAAG	1 cut(s)	668
VneI	GTGCAC	2 cut(s)	745, 826
VpaK11BI	GGWCC	1 cut(s)	1634
VspI	ATTAAT	1 cut(s)	731
XagI	CCTNNNNNAGG	1 cut(s)	1016
XapI	RAATTY	9 cut(s)	110, 426, 847, 912, 1023, 1062, 1103, 1259, 1583
XceI	RCATGY	2 cut(s)	528, 815
XmnI	GAANNNNTTC	1 cut(s)	993
XspI	CTAG	1 cut(s)	1343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.