Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0013431

Physical Location & Seq

Genomic Coordinates Forward (+)

34798867 .. 34804069

Gene Structure 5203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00242490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGGATTCCGACTACGGCATTCCCCGAGAGCTCTCCGACGTCCAGAAGCTCCGATCTCAATACCAGCCGGAGCTTCCTCCTTGCCTTCAGGGAACTACTGTAAGAGTTGAATTTGGGGACACAACAACAGCTGCAGACCCCACAGATGCCAATGCCATTGCTCGATACTTTCCACACACTTATGGTCAGCCTTTGGCTCACTTTCTCAGGGCAACTGCAAAAAAAGTTGCTGGTGCTCAGGTTATTACTGAGCATCCAGCTATGAGGGTCGGAATTGTCTTTTGTGGGAGACAATCTCCTGGAGGACACAATGTCATCTGGGGCCTTCTCAAGGCTCTCAAAATTCATAACCCCAAAAGTACCTTAATTGGGTTTTTGGGTGGTTCTGAAGGTTTATTTGCACAAAAAACTCTTGAGATAACAGATGATGTTCTTGCCACCTACAAAAATCAAGGCGGTTATGATTTGCTTGGACGGACAAGGGATCAAATTAGAACAAAGGAGCAAGTCAACGCTACGTTAACTGCATGCAATGCTATGAAGTTGGATGGCCTTGTCATAATTGGAGGGGTGACATCAAACACTGATGCCGCCCAGCTTGCAGAGACATTCGCCGAAGCAAAATGCCAGACAAAGGTGGTTGGGGTTCCTGTCACTATGAACGGAGACCTTAAGAACCAGTTTGTGGAAGCTAATGTTGGTTTTGATACGGTGTGCAAGGTCAATTCCCAGCTCATCAGCAATATGTGCACAGATGCTCTTTCTGCTGAAAAGTATTATTATTTCATTCGACTTATGGGCCGGAAGGCATCACATGTTGCCTTGGAGTGCACCCTTCAATCTCATCCAAACTTGGTAATTCTTGGTGAGGAGGTGGCTGCATCCAAGCTAACACTTTTTGACATTACAACCCGAATCTGTGATGCAATCCAAGATCGTGCCACACAAGAGAAGAATCATGGTGTCATCCTATTACCGGAAGGACTCATAGAAAGCATTCCTGAAGTGTATGCCCTCTTGAAGGAAATTCATGGTTTGTTTAGGCAAGGAGTTTCTGCCGATAAGATTTCTTCTCAACTCTCACCGTGGGCTTCTGCTCTGTTTGAATTTTTGCCACATTTTATCAAGAAACAGCTGCTTCTTTTGCCTGAATCAGATGACTCTGCACAGTTATCTCAGATTGAGACAGAGAAACTTTTTGCACATCTTGTGGAGGTCGAGATGAACAAGCGCCAGAAAGAAGGCACATACAAGGGGAAGAAGTTTAATGCTATCTGCCACTTTTTCGGTTATCAGGCTCGGGGGTCTTTACCATCAAAATTCGATTGTGACTATGCCTATGTTCTAGGGCACATCTGCTACCACATTCTTGCTGCTGGTTTGAATGGTTACATGGCAACTATAACTAACCTGAAAAATCCATTGAACAAGTGGCGTTGTGGCGCTGCTCCACTTACGGCAATGATGACTGTGAAGCGCTGGTCTCAAAATCCTGGGTCTACTGCATCTATTGGAAAACCTGGCATTTTTCCAGCTATCGTAGATTTGAAAGGCAAAGCATATGAGCTGCTGAGAAAAAATGCTACAAAATTCCTGCTGGATGATATCTACAGAAATCCTGGGCCTCTTCAATTTGAAGGTCCTGGTGCTGATTCTAAGGCTGTAACACTGTGCGTTGAAGACCAGGATTACATGGGGCGCATCAAGAAATTGCAGGAATATCTTGACCAGATTCGAACCATTGTAAAGCCTGGGTGCTCACAGGAGGTTCTGAAAGCAGCTTTGAGTGTCATGGGCTCTGTAACAGAGGTCCTAACTGTTATGTCCTCATCTTCTTTGAATGCCCCGTCATCTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008443 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010033 GO:0015979 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0034284 GO:0034285 GO:0042221 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0047334 GO:0050896 GO:0071704 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko01100 ko01110 ko01120 ko01130 map00010 map00030 map00051 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

PFK

Protein Analysis

619

Amino Acids

67.84

Weight (kDa)

8.07

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	89 - 400	1.6e-27	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20950 AT1G76550
fragaria_vesca	FvH4_5g26380 FvH4_5g26380
malus_domestica	MD06G1002300.v1.1
prunus_persica	Prupe.5G003400_v2.0.a1
pyrus_communis	pycom06g00240
rosa_chinensis	RchiOBHm_Chr7g0217221
rosa_laevigata	RLG00000002501
rosa_multiflora	Rmu_sc0007586.1_g000002 Rmu_sc0022408.1_g000001
rosa_roxburghii	Rroxscaffold_3G00242490
rosa_rugosa	Rorug07G0167300
rosa_samantha	Rh7AG306300 Rh7BG297600 Rh7CG325400 Rh7DG306100
rosa_wichuraiana	Rw7G026020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	42
AccB7I	CCANNNNNTGG	1 cut(s)	685
AccI	GTMKAC	1 cut(s)	1499
AciI	CCGC	2 cut(s)	456, 591
AclWI	GGATC	1 cut(s)	492
AcsI	RAATTY	6 cut(s)	110, 342, 1026, 1106, 1319, 1589
AcuI	CTGAAG	3 cut(s)	71, 408, 1023
AcyI	GRCGYC	1 cut(s)	39
AfaI	GTAC	1 cut(s)	361
AfeI	AGCGCT	1 cut(s)	1478
AfiI	CCNNNNNNNGG	6 cut(s)	331, 369, 634, 685, 976, 1021
AflII	CTTAAG	1 cut(s)	671
AflIII	ACRYGT	1 cut(s)	814
AhdI	GACNNNNNGTC	1 cut(s)	311
AjnI	CCWGG	7 cut(s)	298, 1492, 1519, 1618, 1642, 1683, 1750
AjuI	GAANNNNNNNTTGG	2 cut(s)	681, 713
Alw21I	GWGCWC	5 cut(s)	33, 238, 752, 833, 1760
Alw26I	GTCTC	5 cut(s)	283, 599, 660, 1178, 1488
Alw44I	GTGCAC	2 cut(s)	748, 829
AlwI	GGATC	1 cut(s)	492
Ama87I	CYCGRG	2 cut(s)	24, 1299
Aor51HI	AGCGCT	1 cut(s)	1478
AoxI	GGCC	4 cut(s)	322, 550, 799, 1622
ApaLI	GTGCAC	2 cut(s)	748, 829
ApeKI	GCWGC	7 cut(s)	131, 878, 1135, 1373, 1445, 1567, 1778
ApoI	RAATTY	6 cut(s)	110, 342, 1026, 1106, 1319, 1589
Asp700I	GAANNNNTTC	1 cut(s)	996
AspLEI	GCGC	4 cut(s)	1233, 1445, 1479, 1701
AspS9I	GGNCC	5 cut(s)	322, 799, 1622, 1640, 1810
AsuHPI	GGTGA	3 cut(s)	583, 878, 1074
AsuII	TTCGAA	1 cut(s)	1735
AvaI	CYCGRG	2 cut(s)	24, 1299
AvaII	GGWCC	2 cut(s)	1640, 1810
BaeGI	GKGCMC	3 cut(s)	752, 833, 1353
BanII	GRGCYC	2 cut(s)	33, 1799
BbsI	GAAGAC	1 cut(s)	1686
Bbv12I	GWGCWC	5 cut(s)	33, 238, 752, 833, 1760
BbvI	GCAGC	7 cut(s)	118, 865, 1122, 1360, 1432, 1554, 1790
BccI	CCATC	2 cut(s)	542, 1321
BceAI	ACGGC	2 cut(s)	31, 1473
BciT130I	CCWGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
BcoDI	GTCTC	5 cut(s)	283, 599, 660, 1178, 1488
BfaI	CTAG	1 cut(s)	1346
BfmI	CTRYAG	2 cut(s)	132, 1609
BfoI	RGCGCY	3 cut(s)	1234, 1446, 1480
BfrI	CTTAAG	1 cut(s)	671
BisI	GCNGC	8 cut(s)	132, 591, 879, 1136, 1374, 1446, 1568, 1779
BlsI	GCNGC	8 cut(s)	133, 592, 880, 1137, 1375, 1447, 1569, 1780
Bme1390I	CCNGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
Bme18I	GGWCC	2 cut(s)	1640, 1810
BmeRI	GACNNNNNGTC	1 cut(s)	311
BmeT110I	CYCGRG	2 cut(s)	24, 1299
BmgT120I	GGNCC	5 cut(s)	322, 799, 1622, 1640, 1810
BmiI	GGNNCC	2 cut(s)	323, 648
BmrFI	CCNGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
BmsI	GCATC	9 cut(s)	136, 262, 577, 745, 818, 890, 913, 1514, 1710
BpiI	GAAGAC	1 cut(s)	1686
BplI	GAGNNNNNCTC	2 cut(s)	280, 312
BpmI	CTGGAG	1 cut(s)	321
Bpu10I	CCTNAGC	1 cut(s)	237
Bpu14I	TTCGAA	1 cut(s)	1735
BpuEI	CTTGAG	2 cut(s)	314, 434
BsaHI	GRCGYC	1 cut(s)	39
BsaI	GGTCTC	2 cut(s)	660, 1488
BsaJI	CCNNGG	5 cut(s)	822, 1085, 1493, 1619, 1751
BsaWI	WCCGGW	1 cut(s)	976
Bsc4I	CCNNNNNNNGG	6 cut(s)	331, 369, 634, 685, 976, 1021
Bse1I	ACTGG	1 cut(s)	679
Bse3DI	GCAATG	3 cut(s)	156, 538, 1467
BseBI	CCWGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
BseDI	CCNNGG	5 cut(s)	822, 1085, 1493, 1619, 1751
BseGI	GGATG	6 cut(s)	253, 553, 844, 881, 966, 1606
BseLI	CCNNNNNNNGG	6 cut(s)	331, 369, 634, 685, 976, 1021
BseMI	GCAATG	3 cut(s)	156, 538, 1467
BseMII	CTCAG	5 cut(s)	220, 240, 251, 1190, 1562
BseNI	ACTGG	1 cut(s)	679
BseRI	GAGGAG	1 cut(s)	884
BseSI	GKGCMC	3 cut(s)	752, 833, 1353
BseXI	GCAGC	7 cut(s)	118, 865, 1122, 1360, 1432, 1554, 1790
BseYI	CCCAGC	2 cut(s)	594, 729
BsgI	GTGCAG	1 cut(s)	1149
BshFI	GGCC	4 cut(s)	324, 552, 801, 1624
BsiHKAI	GWGCWC	5 cut(s)	33, 238, 752, 833, 1760
BsiHKCI	CYCGRG	2 cut(s)	24, 1299
BsiSI	CCGG	3 cut(s)	68, 802, 977
BslFI	GGGAC	1 cut(s)	131
BslI	CCNNNNNNNGG	6 cut(s)	331, 369, 634, 685, 976, 1021
BsmAI	GTCTC	5 cut(s)	283, 599, 660, 1178, 1488
BsmFI	GGGAC	1 cut(s)	131
BsmI	GAATGC	3 cut(s)	18, 996, 1846
BsnI	GGCC	4 cut(s)	324, 552, 801, 1624
Bso31I	GGTCTC	2 cut(s)	660, 1488
BsoBI	CYCGRG	2 cut(s)	24, 1299
Bsp119I	TTCGAA	1 cut(s)	1735
Bsp1286I	GDGCHC	7 cut(s)	33, 238, 752, 833, 1353, 1760, 1799
Bsp143I	GATC	3 cut(s)	53, 484, 934
BspACI	CCGC	2 cut(s)	456, 591
BspANI	GGCC	4 cut(s)	324, 552, 801, 1624
BspCNI	CTCAG	5 cut(s)	219, 241, 250, 1189, 1563
BspLI	GGNNCC	2 cut(s)	323, 648
BspMAI	CTGCAG	1 cut(s)	136
BspPI	GGATC	1 cut(s)	492
BspT104I	TTCGAA	1 cut(s)	1735
BspTI	CTTAAG	1 cut(s)	671
BspTNI	GGTCTC	2 cut(s)	660, 1488
BsrDI	GCAATG	3 cut(s)	156, 538, 1467
BsrI	ACTGG	1 cut(s)	679
BssECI	CCNNGG	5 cut(s)	822, 1085, 1493, 1619, 1751
BssMI	GATC	3 cut(s)	53, 484, 934
BssNI	GRCGYC	1 cut(s)	39
BssT1I	CCWWGG	1 cut(s)	822
Bst2UI	CCWGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
Bst4CI	ACNGT	7 cut(s)	100, 712, 1086, 1170, 1471, 1671, 1819
Bst6I	CTCTTC	1 cut(s)	1632
BstACI	GRCGYC	1 cut(s)	39
BstAFI	CTTAAG	1 cut(s)	671
BstAPI	GCANNNNNTGC	2 cut(s)	533, 747
BstBI	TTCGAA	1 cut(s)	1735
BstC8I	GCNNGC	2 cut(s)	529, 600
BstDEI	CTNAG	6 cut(s)	206, 237, 249, 1176, 1571, 1656
BstDSI	CCRYGG	1 cut(s)	1085
BstENI	CCTNNNNNAGG	2 cut(s)	329, 1019
BstF5I	GGATG	6 cut(s)	253, 553, 844, 881, 966, 1606
BstH2I	RGCGCY	3 cut(s)	1234, 1446, 1480
BstHHI	GCGC	4 cut(s)	1233, 1445, 1479, 1701
BstKTI	GATC	3 cut(s)	56, 487, 937
BstMAI	GTCTC	5 cut(s)	283, 599, 660, 1178, 1488
BstMBI	GATC	3 cut(s)	53, 484, 934
BstMWI	GCNNNNNNNGC	5 cut(s)	533, 599, 747, 764, 1144
BstNI	CCWGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
BstNSI	RCATGY	2 cut(s)	531, 818
BstSCI	CCNGG	7 cut(s)	298, 1492, 1519, 1618, 1642, 1683, 1750
BstSFI	CTRYAG	2 cut(s)	132, 1609
BstSLI	GKGCMC	3 cut(s)	752, 833, 1353
BstV1I	GCAGC	7 cut(s)	118, 865, 1122, 1360, 1432, 1554, 1790
BstV2I	GAAGAC	1 cut(s)	1686
BsuRI	GGCC	4 cut(s)	324, 552, 801, 1624
BtgI	CCRYGG	1 cut(s)	1085
BtsCI	GGATG	6 cut(s)	253, 553, 844, 881, 966, 1606
BtsIMutI	CAGTG	2 cut(s)	582, 1667
Cac8I	GCNNGC	2 cut(s)	529, 600
CfoI	GCGC	4 cut(s)	1233, 1445, 1479, 1701
Cfr13I	GGNCC	5 cut(s)	322, 799, 1622, 1640, 1810
Csp6I	GTAC	1 cut(s)	360
CviAII	CATG	7 cut(s)	528, 815, 959, 1031, 1393, 1693, 1792
CviQI	GTAC	1 cut(s)	360
DdeI	CTNAG	6 cut(s)	206, 237, 249, 1176, 1571, 1656
DpnI	GATC	3 cut(s)	55, 486, 936
DpnII	GATC	3 cut(s)	53, 484, 934
DriI	GACNNNNNGTC	1 cut(s)	311
Eam1104I	CTCTTC	1 cut(s)	1632
Eam1105I	GACNNNNNGTC	1 cut(s)	311
EarI	CTCTTC	1 cut(s)	1632
Ecl136II	GAGCTC	1 cut(s)	31
Eco130I	CCWWGG	1 cut(s)	822
Eco24I	GRGCYC	2 cut(s)	33, 1799
Eco31I	GGTCTC	2 cut(s)	660, 1488
Eco32I	GATATC	1 cut(s)	1606
Eco47I	GGWCC	2 cut(s)	1640, 1810
Eco47III	AGCGCT	1 cut(s)	1478
Eco53kI	GAGCTC	1 cut(s)	31
Eco57I	CTGAAG	3 cut(s)	71, 408, 1023
Eco88I	CYCGRG	2 cut(s)	24, 1299
EcoICRI	GAGCTC	1 cut(s)	31
EcoNI	CCTNNNNNAGG	2 cut(s)	329, 1019
EcoO109I	RGGNCCY	3 cut(s)	322, 1640, 1810
EcoRII	CCWGG	7 cut(s)	298, 1492, 1519, 1618, 1642, 1683, 1750
EcoRV	GATATC	1 cut(s)	1606
EcoT14I	CCWWGG	1 cut(s)	822
EcoT38I	GRGCYC	2 cut(s)	33, 1799
ErhI	CCWWGG	1 cut(s)	822
FaeI	CATG	7 cut(s)	531, 818, 962, 1034, 1396, 1696, 1795
FaqI	GGGAC	1 cut(s)	131
FatI	CATG	7 cut(s)	527, 814, 958, 1030, 1392, 1692, 1791
FauNDI	CATATG	1 cut(s)	1561
FblI	GTMKAC	1 cut(s)	1499
Fnu4HI	GCNGC	8 cut(s)	132, 591, 879, 1136, 1374, 1446, 1568, 1779
FokI	GGATG	6 cut(s)	240, 560, 831, 868, 953, 1613
FriOI	GRGCYC	2 cut(s)	33, 1799
Fsp4HI	GCNGC	8 cut(s)	132, 591, 879, 1136, 1374, 1446, 1568, 1779
FspBI	CTAG	1 cut(s)	1346
GlaI	GCGC	4 cut(s)	1232, 1444, 1478, 1700
GluI	GCNGC	8 cut(s)	132, 591, 879, 1136, 1374, 1446, 1568, 1779
GsaI	CCCAGC	2 cut(s)	598, 733
GsuI	CTGGAG	1 cut(s)	321
HaeII	RGCGCY	3 cut(s)	1234, 1446, 1480
HaeIII	GGCC	4 cut(s)	324, 552, 801, 1624
HapII	CCGG	3 cut(s)	68, 802, 977
HhaI	GCGC	4 cut(s)	1233, 1445, 1479, 1701
Hin1I	GRCGYC	1 cut(s)	39
Hin1II	CATG	7 cut(s)	531, 818, 962, 1034, 1396, 1696, 1795
Hin6I	GCGC	4 cut(s)	1231, 1443, 1477, 1699
HinP1I	GCGC	4 cut(s)	1231, 1443, 1477, 1699
HincII	GTYRAC	2 cut(s)	511, 522
HindII	GTYRAC	2 cut(s)	511, 522
HinfI	GANTC	8 cut(s)	5, 915, 955, 984, 1151, 1160, 1652, 1732
HpaI	GTTAAC	1 cut(s)	522
HpaII	CCGG	3 cut(s)	68, 802, 977
HphI	GGTGA	3 cut(s)	583, 878, 1074
Hpy166II	GTNNAC	5 cut(s)	511, 522, 750, 831, 1500
Hpy188I	TCNGA	8 cut(s)	10, 37, 53, 272, 388, 1156, 1179, 1773
Hpy188III	TCNNGA	8 cut(s)	43, 413, 1001, 1018, 1126, 1219, 1705, 1724
Hpy8I	GTNNAC	5 cut(s)	511, 522, 750, 831, 1500
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	9 cut(s)	95, 335, 383, 799, 845, 974, 1015, 1235, 1631
HpyCH4III	ACNGT	7 cut(s)	100, 712, 1086, 1170, 1471, 1671, 1819
HpyCH4IV	ACGT	2 cut(s)	39, 518
HpyF10VI	GCNNNNNNNGC	5 cut(s)	533, 599, 747, 764, 1144
HpyF3I	CTNAG	6 cut(s)	206, 237, 249, 1176, 1571, 1656
HpySE526I	ACGT	2 cut(s)	39, 518
Hsp92I	GRCGYC	1 cut(s)	39
Hsp92II	CATG	7 cut(s)	531, 818, 962, 1034, 1396, 1696, 1795
HspAI	GCGC	4 cut(s)	1231, 1443, 1477, 1699
KspAI	GTTAAC	1 cut(s)	522
Kzo9I	GATC	3 cut(s)	53, 484, 934
LmnI	GCTCC	4 cut(s)	54, 70, 502, 1453
Lsp1109I	GCAGC	7 cut(s)	118, 865, 1122, 1360, 1432, 1554, 1790
LweI	GCATC	9 cut(s)	136, 262, 577, 745, 818, 890, 913, 1514, 1710
MaeI	CTAG	1 cut(s)	1346
MaeII	ACGT	2 cut(s)	39, 518
MaeIII	GTNAC	6 cut(s)	571, 652, 1328, 1388, 1663, 1801
MalI	GATC	3 cut(s)	55, 486, 936
MboI	GATC	3 cut(s)	53, 484, 934
MboII	GAAGA	6 cut(s)	964, 1062, 1270, 1619, 1691, 1824
MhlI	GDGCHC	7 cut(s)	33, 238, 752, 833, 1353, 1760, 1799
MlyI	GAGTC	2 cut(s)	978, 1154
MmeI	TCCRAC	4 cut(s)	33, 60, 250, 525
MroXI	GAANNNNTTC	1 cut(s)	996
MseI	TTAA	5 cut(s)	365, 521, 672, 1266, 1858
MslI	CAYNNNNRTG	1 cut(s)	180
MspA1I	CMGCKG	2 cut(s)	131, 1135
MspCI	CTTAAG	1 cut(s)	671
MspI	CCGG	3 cut(s)	68, 802, 977
MspR9I	CCNGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
Mva1269I	GAATGC	3 cut(s)	18, 996, 1846
MvaI	CCWGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
MwoI	GCNNNNNNNGC	5 cut(s)	533, 599, 747, 764, 1144
NdeI	CATATG	1 cut(s)	1561
NdeII	GATC	3 cut(s)	53, 484, 934
NlaIII	CATG	7 cut(s)	531, 818, 962, 1034, 1396, 1696, 1795
NlaIV	GGNNCC	2 cut(s)	323, 648
NmuCI	GTSAC	3 cut(s)	571, 652, 1328
NspI	RCATGY	2 cut(s)	531, 818
NspV	TTCGAA	1 cut(s)	1735
PaeI	GCATGC	1 cut(s)	531
PciI	ACATGT	1 cut(s)	814
PctI	GAATGC	3 cut(s)	18, 996, 1846
PdmI	GAANNNNTTC	1 cut(s)	996
PfeI	GAWTC	6 cut(s)	5, 915, 955, 1151, 1652, 1732
PflMI	CCANNNNNTGG	1 cut(s)	685
PfoI	TCCNGGA	1 cut(s)	298
PkrI	GCNGC	8 cut(s)	133, 592, 880, 1137, 1375, 1447, 1569, 1780
PleI	GAGTC	2 cut(s)	978, 1154
PpsI	GAGTC	2 cut(s)	978, 1154
PpuMI	RGGWCCY	2 cut(s)	1640, 1810
PscI	ACATGT	1 cut(s)	814
Psp124BI	GAGCTC	1 cut(s)	33
Psp5II	RGGWCCY	2 cut(s)	1640, 1810
Psp6I	CCWGG	7 cut(s)	298, 1492, 1519, 1618, 1642, 1683, 1750
PspFI	CCCAGC	2 cut(s)	594, 729
PspGI	CCWGG	7 cut(s)	298, 1492, 1519, 1618, 1642, 1683, 1750
PspN4I	GGNNCC	2 cut(s)	323, 648
PspPI	GGNCC	5 cut(s)	322, 799, 1622, 1640, 1810
PspPPI	RGGWCCY	2 cut(s)	1640, 1810
PstI	CTGCAG	1 cut(s)	136
PvuII	CAGCTG	2 cut(s)	131, 1135
RsaI	GTAC	1 cut(s)	361
RsaNI	GTAC	1 cut(s)	360
RseI	CAYNNNNRTG	1 cut(s)	180
SacI	GAGCTC	1 cut(s)	33
SaqAI	TTAA	5 cut(s)	365, 521, 672, 1266, 1858
SatI	GCNGC	8 cut(s)	132, 591, 879, 1136, 1374, 1446, 1568, 1779
Sau3AI	GATC	3 cut(s)	53, 484, 934
Sau96I	GGNCC	5 cut(s)	322, 799, 1622, 1640, 1810
SchI	GAGTC	2 cut(s)	978, 1154
ScrFI	CCNGG	7 cut(s)	300, 1494, 1521, 1620, 1644, 1685, 1752
SduI	GDGCHC	7 cut(s)	33, 238, 752, 833, 1353, 1760, 1799
SfaNI	GCATC	9 cut(s)	136, 262, 577, 745, 818, 890, 913, 1514, 1710
SfcI	CTRYAG	2 cut(s)	132, 1609
SfuI	TTCGAA	1 cut(s)	1735
SinI	GGWCC	2 cut(s)	1640, 1810
SmiMI	CAYNNNNRTG	1 cut(s)	180
SmlI	CTYRAG	3 cut(s)	329, 413, 671
SmoI	CTYRAG	3 cut(s)	329, 413, 671
SphI	GCATGC	1 cut(s)	531
SsiI	CCGC	2 cut(s)	456, 591
SspMI	CTAG	1 cut(s)	1346
SstI	GAGCTC	1 cut(s)	33
StyD4I	CCNGG	7 cut(s)	298, 1492, 1519, 1618, 1642, 1683, 1750
StyI	CCWWGG	1 cut(s)	822
TaaI	ACNGT	7 cut(s)	100, 712, 1086, 1170, 1471, 1671, 1819
TaiI	ACGT	2 cut(s)	42, 521
TaqI	TCGA	5 cut(s)	163, 790, 1218, 1323, 1735
TauI	GCSGC	1 cut(s)	593
TfiI	GAWTC	6 cut(s)	5, 915, 955, 1151, 1652, 1732
Tru1I	TTAA	5 cut(s)	365, 521, 672, 1266, 1858
Tru9I	TTAA	5 cut(s)	365, 521, 672, 1266, 1858
TscAI	CASTG	2 cut(s)	589, 1674
TseFI	GTSAC	3 cut(s)	571, 652, 1328
TseI	GCWGC	7 cut(s)	131, 878, 1135, 1373, 1445, 1567, 1778
Tsp45I	GTSAC	3 cut(s)	571, 652, 1328
TspDTI	ATGAA	6 cut(s)	335, 554, 674, 775, 1019, 1238
TspGWI	ACGGA	2 cut(s)	490, 678
TspRI	CASTG	2 cut(s)	589, 1674
Van91I	CCANNNNNTGG	1 cut(s)	685
Vha464I	CTTAAG	1 cut(s)	671
VneI	GTGCAC	2 cut(s)	748, 829
VpaK11BI	GGWCC	2 cut(s)	1640, 1810
XagI	CCTNNNNNAGG	2 cut(s)	329, 1019
XapI	RAATTY	6 cut(s)	110, 342, 1026, 1106, 1319, 1589
XceI	RCATGY	2 cut(s)	531, 818
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1428
XmiI	GTMKAC	1 cut(s)	1499
XmnI	GAANNNNTTC	1 cut(s)	996
XspI	CTAG	1 cut(s)	1346
ZraI	GACGTC	1 cut(s)	40

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.