Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013558

Physical Location & Seq

Genomic Coordinates Forward (+)

2096463 .. 2107175

Gene Structure 10713 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1017200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3732 bp

ATGTCACTTCTGAGTAGATTCTTTTACAAAAGACCCCCAGATGGGTTGCTCGAATTCGTAGAGAGAGTATACGTATTTGATTCCTGTTTTTCCACTGAAGTCTTGCCTGATGAAATGTACCAAATATATCTACATGAAATCATTACTGAGTTACATGAAGAATTCCCCGACTCGTCCTTCCTTGCATTCAACTTTCGGGAGGGTGAGAAACGTAGCCAATTTGCTGAGATATTATGCGAATATGATGTTACAGTTATGGATTATCCGCGACAGTACGAGGGTTGTCCACTCCTTCCATTATCTCTTATTCAACATTTTCTTCGTGTTTGTGAGAGTTGGCTCTTGAATGACAAGCAACAAAATGTTATTCTTCTCCATTGTGAGAGGGGAGGTTGGCCACTCTTGGCTTTCCTTTTAGCTAGCTTTCTCATTTTCAGAAAACTGCATAGTGGGGAACGCAAGACGCTTGAAATTGTACATCGAGAAGCTCCTAAAGGTTTATTGCAGCTTTTGTCGCCTTTAAATCCTCTCCCATCTCAGCTGCGGTACCTTCAGTACGTGGCAAGGAGAAATATAGCTCCAGAGTGGCCACCTCCTGAGCGGGCACTTTCTTTAGACTGTGTTATACTTAGAGCCATTCCAAACTTTGATACCCAAAAAGGCTGCACTCCAGTTATCAGAATTTTTGGTCGGAATCTCCTTAGTAAGGGCGGGCTTTCAACCCAGATGATATTCTCCATGCCTAGGAAGAAGACACTTCGGAACTACCGTCAAGCAGACTGTGATGTCATCAAAATTGACATTCAGTGTTTAGTGCAAGGAGATGTTGTATTGGAGTGTGTCCACTTGGATTTGGATCCAGAGAGGGAAGTTATGATGTTCCGTATTATGTTCAACACTGCATTTATTCGTTCCAACATACTGATGCTGAATTCTGATAACTTGGACATTCTATGGGATGCAAAGGAGCGCTATCCAAAGGGCTTCCGAGCTGAGGTATTGTTTGGGGAGTTTGAGAGCATTTCTCCTCCGAGAGCTCCAACTACAACTTTGAATGGTGAGGAGAAAGGTGGATTGCCCCATGATACTTTTTCTAGGGTTCAAGAACTTTTCAATGGAGTTGAGTGGATTGATAGCAATGATGATGCGGCCTTGTGGTTTCTTAAACAGCTCTCTGTCCTAAGTGATGCGAAAGAATTGTCAAGGTTTCAAAACAAAATGAGTTTATATTCATCACCTGCTGACTCTGAAGAAGAAAATAATGCATCTAGCACTGCTGACAGTGCTGATGAAGCATTTGACACTGTTTCCAAGGCTTCAGCAGATTCAACAAAAGCATTGATGCTGGACAGTTTTGATTCTGCCCCCTTATCATATGAGAATGATGGCCCACCTGATGCGAACTTTACTTCAGAGCCTCCTGATCAAGAATCATTTGGACCCGTCCTGGATTCTCTTCATCAGCAATCATTAGATACAAGTAAAGGACCTCCACCTTGTTCATTCCCTGAATCATTGGCACCAAAATCCCTATCAACAGAACCATTATGTCTTGCTCTTCACACTCTACCATCACCTCCACCGCCACCACCGCCTCCACCGCCACCACTTCCTTTTATTGGTGTTAGTAGAGGGTCTTCACCATCCCCACCACTTCCACCCCTTAGCATATCCAGTAAAGATTCTGTGAACCGCTTACATTTGGCTACTACAAGAAGTCCACCACCGCCGCCACCACCACCACCCCTTGTTCCATCTCATTTTGTCTCTAGCAAAAGCCCACTGCCACCTCCTCCCCCACCTCCACCAACTTCTTCTACAGTGCCACCGCCGACCCCACCGACTCCTCCCTTTAAGTCTAGTCAGCAGTCTTTGCCGTCACCACCACCACCACCTCCACCACCTCCACCTCCTCCGTTTTTGGGAAGTACAACTAACAAGCTACCGCCACCCCCGCCTCCCCCTCCTCCTCCACTGTCCACTTCTAGTTCTGTTTTTAGAGCACCTCCAGCTCCATCACCTTCTCCTATGAGTGGTAGCCTTAGTACTAATAATAGGGCACCACCCCCGCCTCCTCCTCCTCCACCATCCACTTCTAGCTCTGTTTTTGGAGCACCGCCAGCTCCACCACCTCCTCCTATGAGTGGTAGCCTTGGTACTAATAGTAGGGCACCGCCCCCGCCTCCTCCTCCTCCACCGTCCATTTCTAGTTCTGGGGTTGGAGCACCCCCACCTCCACCTCCTCCTCCTATGAGTGGTAGTGTTGGTAATTATACTAGGTCACCACCCCCACCTCCACCTCCTTCAGGACCTACAAGGCCAACCCCACCTCCTCCACCACCAGCACCAATGCCACCAAGTCGCGGTGCAACAACATCTGTGCCTCCACCACCTGGAGCAAAGGGCTCTAGTGCACCACCACCACCGCCACCATCAGTTGGAAGGGGCAAAGCGTCTTCTGGGCTGGGGATTGTTGGAAAAGGCCGTGCTACTTCTATTGCCCCAAAGAAAACTTCCTTAAAGCCCTTACACTGGGTGAAAGTTAATCGAGCAATGAAAGAGAGTTTATGGGAGGATTTTCAGAAACAAGGCAATCAGCCAAGGGCTCCTGAAATAGATATATCAGAACTTGAAAGTCTGTTCTCAGCAGCCGCTGCTTCAGATGTGCATGGCAGTAAGACTGCTGGTCGACGTGGTCCTAACATTACCAAACCTGAGAAAGTGCAACTGATTGACTTGCGACGAGCGTATAACTGTGAAATTATGCTTTCAAAAGTAAAGATCCCCTTGCCAGATATGATTAATGCAGTTCTAGCTCTGGATTCTTCTGTTCTTGACATTGATCAGGTTGAGAATCTCATTAAATTTTGTCCTACCAAAGAAGAAATGGAAACTTTGAAGAACTATGCAGGCGACAAGGAAATGCTGGGGAAGTGTGAACAGTTTTTCGTAGAGCTAATGAAGGTTCCACGAGTAGAATCCAAGTTACGAGTATTTGCTTTCAAAATTACCTTTTCTAGTCAGGTGAATGATTTAAGATCTAATCTGAATACAATCAATGGTGCTGCTAATGAGGTGAAGGAATCTGTGAAATTGCGACAGATAATGCAGACAATCCTTACTTTGGGAAATGCCTTGAATCAAGGCACTGCTCGAGGATCTGCTGTAGGATTCAAATTGGACAGCCTTCTTAAATTGTCTGATACCCGTGCAAGAAACAATAAAATGACTTTAATGCATTATTTGTGTAAGCTCCTTGCTGAGAAAATGCCAGAGTTGCTGGATTTTGATAAAGATCTGATTCATTTAGAAGCTGCTTCTAAGATTCAATTGAAAGCTTTGGCTGAAGAAATGCAAGCTGTGAGTAAAGGTCTGGAAAAGGTTGAGCAAGAACTTGCTGCTTCAGAAAACGATGGAGCAATCTCTGCTGGTTTCCAAAAAGTGCTGAAGAATTTTCTTGATACTGCTGAAGCTGATGTAAGGTCGTTAATCTCCTTGTACTCTGAAGTGGGAAGAAATGCAGATTCTTTGTCTCAATACTTCGGGGAGGATCCAGCTCGGTGTCCTTTTGAACAAGTGACACATGTCTTGTTCGTTTTCGTCAAGATGTTCAAAAAGTCACGAGAGGAAAATGAAAGACTTGCTGAGGCCGAAAAAAAGAAGCTTGAAAGGGAAGCCATTAAAGACCGGACAGTAACCAATTCATCTGCAAGGAAAGATGATGTTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0032501 GO:0032502 GO:0034293 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0048229 GO:0048236 GO:0048856 GO:0048869 GO:0051015 GO:0051017 GO:0051321 GO:0051704 GO:0061572 GO:0071840 GO:0097435 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2 PTEN_C2

Protein Analysis

1244

Amino Acids

135.93

Weight (kDa)

6.36

Isoelectric Point (pI)

62.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PTEN_C2	PF10409	198 - 337	4.3e-37	C2 domain of PTEN tumour-suppressor protein
FH2	PF02181	834 - 1205	4.2e-121	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013558)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31810 AT1G31810
fragaria_vesca	FvH4_5g24110 FvH4_5g24110
malus_domestica	MD06G1017200.v1.1
prunus_persica	Prupe.5G019200_v2.0.a1
pyrus_communis	pycom06g01390
rosa_chinensis	RchiOBHm_Chr7g0213641
rosa_laevigata	RLG00000002794
rosa_multiflora	Rmu_sc0008101.1_g000010
rosa_roxburghii	Rroxscaffold_3G00245720
rosa_rugosa	Rorug07G0143800 Rorug07G0143900
rosa_samantha	Rh7AG276700 Rh7BG270900 Rh7CG295700 Rh7DG283000
rosa_wichuraiana	Rw7G023880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1246
AasI	GACNNNNNNGTC	1 cut(s)	785
Acc36I	ACCTGC	1 cut(s)	1246
Acc65I	GGTACC	1 cut(s)	546
AccB1I	GGYRCC	4 cut(s)	546, 1519, 2059, 2170
AccB7I	CCANNNNNTGG	2 cut(s)	2393, 2438
AccBSI	CCGCTC	1 cut(s)	601
AccI	GTMKAC	2 cut(s)	69, 2689
AccII	CGCG	2 cut(s)	268, 2364
AclWI	GGATC	6 cut(s)	851, 864, 2776, 3166, 3545, 3558
AcoI	YGGCCR	2 cut(s)	395, 587
AcsI	RAATTY	6 cut(s)	53, 161, 681, 931, 2864, 3451
AdeI	CACNNNGTG	1 cut(s)	2536
AfaI	GTAC	9 cut(s)	119, 275, 477, 548, 557, 1930, 2047, 2158, 3500
AfeI	AGCGCT	1 cut(s)	971
AflIII	ACRYGT	1 cut(s)	3583
AhdI	GACNNNNNGTC	1 cut(s)	2685
AjiI	CACGTC	1 cut(s)	2693
AjnI	CCWGG	2 cut(s)	1446, 2392
AjuI	GAANNNNNNNTTGG	2 cut(s)	2497, 2529
Alw21I	GWGCWC	5 cut(s)	1039, 2005, 2116, 2227, 2416
Alw26I	GTCTC	2 cut(s)	1771, 3537
Alw44I	GTGCAC	1 cut(s)	2412
AlwI	GGATC	6 cut(s)	851, 864, 2776, 3166, 3545, 3558
AlwNI	CAGNNNCTG	1 cut(s)	2654
Ama87I	CYCGRG	1 cut(s)	3153
Aor51HI	AGCGCT	1 cut(s)	971
AoxI	GGCC	7 cut(s)	395, 587, 1149, 1387, 2318, 2482, 3648
ApaLI	GTGCAC	1 cut(s)	2412
ApeKI	GCWGC	8 cut(s)	505, 541, 663, 2648, 2654, 3065, 3314, 3398
ApoI	RAATTY	6 cut(s)	53, 161, 681, 931, 2864, 3451
ArsI	GACNNNNNNTTYG	2 cut(s)	1184, 1216
AseI	ATTAAT	1 cut(s)	2802
Asp718I	GGTACC	1 cut(s)	546
AspA2I	CCTAGG	1 cut(s)	743
AspLEI	GCGC	1 cut(s)	972
AspS9I	GGNCC	5 cut(s)	1388, 1439, 1487, 2309, 2696
AsuNHI	GCTAGC	1 cut(s)	419
AvaI	CYCGRG	1 cut(s)	3153
AvaII	GGWCC	4 cut(s)	1439, 1487, 2309, 2696
AvrII	CCTAGG	1 cut(s)	743
BaeGI	GKGCMC	4 cut(s)	607, 2062, 2173, 2416
BalI	TGGCCA	2 cut(s)	397, 589
BamHI	GGATCC	2 cut(s)	856, 3550
BanI	GGYRCC	4 cut(s)	546, 1519, 2059, 2170
BanII	GRGCYC	3 cut(s)	1039, 2408, 2608
BarI	GAAGNNNNNNTAC	2 cut(s)	1621, 1653
BauI	CACGAG	2 cut(s)	2970, 3621
BbsI	GAAGAC	3 cut(s)	758, 1629, 2448
Bbv12I	GWGCWC	5 cut(s)	1039, 2005, 2116, 2227, 2416
BbvCI	CCTCAGC	2 cut(s)	993, 3645
BbvI	GCAGC	8 cut(s)	517, 528, 650, 2641, 2660, 3052, 3301, 3385
BceAI	ACGGC	2 cut(s)	1861, 2469
BciT130I	CCWGG	2 cut(s)	1448, 2394
BclI	TGATCA	2 cut(s)	1423, 2842
BcoDI	GTCTC	2 cut(s)	1771, 3537
BfmI	CTRYAG	2 cut(s)	1818, 3165
BfoI	RGCGCY	1 cut(s)	973
BfuAI	ACCTGC	1 cut(s)	1246
BglII	AGATCT	2 cut(s)	3038, 3295
BlnI	CCTAGG	1 cut(s)	743
BmcAI	AGTACT	1 cut(s)	2047
Bme1390I	CCNGG	2 cut(s)	1448, 2394
Bme18I	GGWCC	4 cut(s)	1439, 1487, 2309, 2696
BmeRI	GACNNNNNGTC	1 cut(s)	2685
BmeT110I	CYCGRG	1 cut(s)	3153
BmgBI	CACGTC	1 cut(s)	2693
BmgT120I	GGNCC	5 cut(s)	1388, 1439, 1487, 2309, 2696
BmiI	GGNNCC	9 cut(s)	548, 858, 1441, 1521, 2061, 2172, 2607, 2967, 3552
BmrFI	CCNGG	2 cut(s)	1448, 2394
BmrI	ACTGGG	1 cut(s)	2542
BmsI	GCATC	7 cut(s)	915, 949, 1135, 1177, 1274, 1332, 1387
BmtI	GCTAGC	1 cut(s)	423
BmuI	ACTGGG	1 cut(s)	2542
BoxI	GACNNNNGTC	1 cut(s)	3584
BpiI	GAAGAC	3 cut(s)	758, 1629, 2448
BplI	GAGNNNNNCTC	2 cut(s)	1009, 1041
BpmI	CTGGAG	4 cut(s)	564, 654, 1992, 2415
Bpu10I	CCTNAGC	4 cut(s)	597, 993, 1664, 3645
BsaAI	YACGTR	2 cut(s)	73, 559
BsaBI	GATNNNNATC	2 cut(s)	855, 3294
BsaJI	CCNNGG	4 cut(s)	743, 1311, 2152, 2600
BsaWI	WCCGGW	1 cut(s)	3687
BsaXI	ACNNNNNCTCC	4 cut(s)	813, 843, 2314, 2344
Bse1I	ACTGG	3 cut(s)	671, 1674, 2537
Bse3DI	GCAATG	2 cut(s)	1144, 2559
Bse8I	GATNNNNATC	2 cut(s)	855, 3294
BseBI	CCWGG	2 cut(s)	1448, 2394
BseDI	CCNNGG	4 cut(s)	743, 1311, 2152, 2600
BseGI	GGATG	3 cut(s)	964, 1643, 2087
BseJI	GATNNNNATC	2 cut(s)	855, 3294
BseMI	GCAATG	2 cut(s)	1144, 2559
BseMII	CTCAG	9 cut(s)	138, 216, 551, 588, 984, 2658, 2706, 3252, 3636
BseNI	ACTGG	3 cut(s)	671, 1674, 2537
BseSI	GKGCMC	4 cut(s)	607, 2062, 2173, 2416
BseXI	GCAGC	8 cut(s)	517, 528, 650, 2641, 2660, 3052, 3301, 3385
BseYI	CCCAGC	2 cut(s)	2464, 2926
BsgI	GTGCAG	1 cut(s)	649
Bsh1236I	CGCG	2 cut(s)	268, 2364
BshFI	GGCC	7 cut(s)	397, 589, 1151, 1389, 2320, 2484, 3650
BshNI	GGYRCC	4 cut(s)	546, 1519, 2059, 2170
BsiHKAI	GWGCWC	5 cut(s)	1039, 2005, 2116, 2227, 2416
BsiHKCI	CYCGRG	1 cut(s)	3153
BsiSI	CCGG	1 cut(s)	3688
BsmAI	GTCTC	2 cut(s)	1771, 3537
BsmI	GAATGC	1 cut(s)	185
BsnI	GGCC	7 cut(s)	397, 589, 1151, 1389, 2320, 2484, 3650
BsoBI	CYCGRG	1 cut(s)	3153
Bsp1407I	TGTACA	1 cut(s)	475
Bsp143I	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
BspANI	GGCC	7 cut(s)	397, 589, 1151, 1389, 2320, 2484, 3650
BspCNI	CTCAG	9 cut(s)	139, 217, 550, 589, 985, 2657, 2707, 3253, 3637
BspFNI	CGCG	2 cut(s)	268, 2364
BspLI	GGNNCC	9 cut(s)	548, 858, 1441, 1521, 2061, 2172, 2607, 2967, 3552
BspMI	ACCTGC	1 cut(s)	1246
BspOI	GCTAGC	1 cut(s)	423
BspPI	GGATC	6 cut(s)	851, 864, 2776, 3166, 3545, 3558
BspQI	GCTCTTC	1 cut(s)	1563
BspT107I	GGYRCC	4 cut(s)	546, 1519, 2059, 2170
BsrBI	CCGCTC	1 cut(s)	601
BsrDI	GCAATG	2 cut(s)	1144, 2559
BsrGI	TGTACA	1 cut(s)	475
BsrI	ACTGG	3 cut(s)	671, 1674, 2537
BssECI	CCNNGG	4 cut(s)	743, 1311, 2152, 2600
BssMI	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
BssNAI	GTATAC	1 cut(s)	70
BssSI	CACGAG	2 cut(s)	2970, 3621
BssT1I	CCWWGG	4 cut(s)	743, 1311, 2152, 2600
Bst1107I	GTATAC	1 cut(s)	70
Bst2BI	CACGAG	2 cut(s)	2970, 3621
Bst2UI	CCWGG	2 cut(s)	1448, 2394
Bst6I	CTCTTC	2 cut(s)	1461, 1563
BstAPI	GCANNNNNTGC	3 cut(s)	1873, 2654, 3425
BstAUI	TGTACA	1 cut(s)	475
BstBAI	YACGTR	2 cut(s)	73, 559
BstC8I	GCNNGC	6 cut(s)	421, 603, 713, 2121, 2911, 3357
BstEII	GGTNACC	1 cut(s)	2280
BstENI	CCTNNNNNAGG	2 cut(s)	704, 2304
BstF5I	GGATG	3 cut(s)	964, 1643, 2087
BstFNI	CGCG	2 cut(s)	268, 2364
BstH2I	RGCGCY	1 cut(s)	973
BstHHI	GCGC	1 cut(s)	972
BstKTI	GATC	8 cut(s)	859, 1426, 2784, 2845, 3041, 3161, 3298, 3553
BstMAI	GTCTC	2 cut(s)	1771, 3537
BstMBI	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
BstNI	CCWGG	2 cut(s)	1448, 2394
BstNSI	RCATGY	1 cut(s)	3587
BstPAI	GACNNNNGTC	1 cut(s)	3584
BstPI	GGTNACC	1 cut(s)	2280
BstSCI	CCNGG	2 cut(s)	1446, 2392
BstSFI	CTRYAG	2 cut(s)	1818, 3165
BstSLI	GKGCMC	4 cut(s)	607, 2062, 2173, 2416
BstSNI	TACGTA	1 cut(s)	73
BstUI	CGCG	2 cut(s)	268, 2364
BstV1I	GCAGC	8 cut(s)	517, 528, 650, 2641, 2660, 3052, 3301, 3385
BstV2I	GAAGAC	3 cut(s)	758, 1629, 2448
BstX2I	RGATCY	6 cut(s)	856, 2781, 3038, 3158, 3295, 3550
BstYI	RGATCY	6 cut(s)	856, 2781, 3038, 3158, 3295, 3550
BstZ17I	GTATAC	1 cut(s)	70
BsuRI	GGCC	7 cut(s)	397, 589, 1151, 1389, 2320, 2484, 3650
BtrI	CACGTC	1 cut(s)	2693
BtsCI	GGATG	3 cut(s)	964, 1643, 2087
BtsI	GCAGTG	4 cut(s)	897, 1272, 1781, 3147
BveI	ACCTGC	1 cut(s)	1246
Cac8I	GCNNGC	6 cut(s)	421, 603, 713, 2121, 2911, 3357
CaiI	CAGNNNCTG	1 cut(s)	2654
CfoI	GCGC	1 cut(s)	972
Cfr13I	GGNCC	5 cut(s)	1388, 1439, 1487, 2309, 2696
CseI	GACGC	2 cut(s)	472, 2442
Csp6I	GTAC	9 cut(s)	118, 274, 476, 547, 556, 1929, 2046, 2157, 3499
CviAII	CATG	6 cut(s)	134, 155, 739, 1082, 2669, 3584
CviQI	GTAC	9 cut(s)	118, 274, 476, 547, 556, 1929, 2046, 2157, 3499
DpnI	GATC	8 cut(s)	858, 1425, 2783, 2844, 3040, 3160, 3297, 3552
DpnII	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
DraI	TTTAAA	1 cut(s)	522
DraIII	CACNNNGTG	1 cut(s)	2536
DrdI	GACNNNNNNGTC	1 cut(s)	785
DriI	GACNNNNNGTC	1 cut(s)	2685
DseDI	GACNNNNNNGTC	1 cut(s)	785
EaeI	YGGCCR	2 cut(s)	395, 587
Eam1104I	CTCTTC	2 cut(s)	1461, 1563
Eam1105I	GACNNNNNGTC	1 cut(s)	2685
EarI	CTCTTC	2 cut(s)	1461, 1563
Ecl136II	GAGCTC	1 cut(s)	1037
Eco105I	TACGTA	1 cut(s)	73
Eco130I	CCWWGG	4 cut(s)	743, 1311, 2152, 2600
Eco24I	GRGCYC	3 cut(s)	1039, 2408, 2608
Eco47I	GGWCC	4 cut(s)	1439, 1487, 2309, 2696
Eco47III	AGCGCT	1 cut(s)	971
Eco53kI	GAGCTC	1 cut(s)	1037
Eco88I	CYCGRG	1 cut(s)	3153
Eco91I	GGTNACC	1 cut(s)	2280
EcoICRI	GAGCTC	1 cut(s)	1037
EcoNI	CCTNNNNNAGG	2 cut(s)	704, 2304
EcoO109I	RGGNCCY	2 cut(s)	1487, 2309
EcoO65I	GGTNACC	1 cut(s)	2280
EcoRI	GAATTC	3 cut(s)	53, 161, 931
EcoRII	CCWGG	2 cut(s)	1446, 2392
EcoT14I	CCWWGG	4 cut(s)	743, 1311, 2152, 2600
EcoT22I	ATGCAT	2 cut(s)	1267, 3240
EcoT38I	GRGCYC	3 cut(s)	1039, 2408, 2608
ErhI	CCWWGG	4 cut(s)	743, 1311, 2152, 2600
FaeI	CATG	6 cut(s)	137, 158, 742, 1085, 2672, 3587
FalI	AAGNNNNNCTT	2 cut(s)	2771, 2803
FatI	CATG	6 cut(s)	133, 154, 738, 1081, 2668, 3583
FauI	CCCGC	5 cut(s)	594, 704, 1962, 2076, 2187
FauNDI	CATATG	1 cut(s)	1375
FbaI	TGATCA	2 cut(s)	1423, 2842
FblI	GTMKAC	2 cut(s)	69, 2689
FokI	GGATG	3 cut(s)	971, 1630, 2074
FriOI	GRGCYC	3 cut(s)	1039, 2408, 2608
GlaI	GCGC	1 cut(s)	971
GsaI	CCCAGC	2 cut(s)	2468, 2930
GsuI	CTGGAG	4 cut(s)	564, 654, 1992, 2415
HaeII	RGCGCY	1 cut(s)	973
HaeIII	GGCC	7 cut(s)	397, 589, 1151, 1389, 2320, 2484, 3650
HapII	CCGG	1 cut(s)	3688
HgaI	GACGC	2 cut(s)	472, 2442
HhaI	GCGC	1 cut(s)	972
Hin1II	CATG	6 cut(s)	137, 158, 742, 1085, 2672, 3587
Hin6I	GCGC	1 cut(s)	970
HinP1I	GCGC	1 cut(s)	970
HincII	GTYRAC	1 cut(s)	2690
HindII	GTYRAC	1 cut(s)	2690
HindIII	AAGCTT	2 cut(s)	3336, 3662
HpaII	CCGG	1 cut(s)	3688
Hpy166II	GTNNAC	9 cut(s)	70, 287, 844, 1690, 1721, 1980, 2414, 2690, 2939
Hpy8I	GTNNAC	9 cut(s)	70, 287, 844, 1690, 1721, 1980, 2414, 2690, 2939
Hpy99I	CGWCG	2 cut(s)	2694, 2745
HpyAV	CCTTC	9 cut(s)	187, 302, 560, 2031, 2313, 2436, 2956, 3073, 3197
HpyCH4IV	ACGT	4 cut(s)	72, 211, 558, 2692
HpySE526I	ACGT	4 cut(s)	72, 211, 558, 2692
Hsp92II	CATG	6 cut(s)	137, 158, 742, 1085, 2672, 3587
HspAI	GCGC	1 cut(s)	970
KpnI	GGTACC	1 cut(s)	550
Ksp22I	TGATCA	2 cut(s)	1423, 2842
Kzo9I	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
LguI	GCTCTTC	1 cut(s)	1563
Lsp1109I	GCAGC	8 cut(s)	517, 528, 650, 2641, 2660, 3052, 3301, 3385
LweI	GCATC	7 cut(s)	915, 949, 1135, 1177, 1274, 1332, 1387
MaeII	ACGT	4 cut(s)	72, 211, 558, 2692
MaeIII	GTNAC	9 cut(s)	3, 150, 247, 1878, 2280, 2985, 3577, 3618, 3694
MalI	GATC	8 cut(s)	858, 1425, 2783, 2844, 3040, 3160, 3297, 3552
MbiI	CCGCTC	1 cut(s)	601
MboI	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
MfeI	CAATTG	1 cut(s)	3329
MflI	RGATCY	6 cut(s)	856, 2781, 3038, 3158, 3295, 3550
MlsI	TGGCCA	2 cut(s)	397, 589
MluNI	TGGCCA	2 cut(s)	397, 589
MlyI	GAGTC	3 cut(s)	164, 1238, 1837
MmeI	TCCRAC	6 cut(s)	671, 939, 1064, 2200, 2419, 2455
Mox20I	TGGCCA	2 cut(s)	397, 589
Mph1103I	ATGCAT	2 cut(s)	1267, 3240
MscI	TGGCCA	2 cut(s)	397, 589
MslI	CAYNNNNRTG	1 cut(s)	923
Msp20I	TGGCCA	2 cut(s)	397, 589
MspA1I	CMGCKG	2 cut(s)	541, 2654
MspI	CCGG	1 cut(s)	3688
MspR9I	CCNGG	2 cut(s)	1448, 2394
MunI	CAATTG	1 cut(s)	3329
Mva1269I	GAATGC	1 cut(s)	185
MvaI	CCWGG	2 cut(s)	1448, 2394
MvnI	CGCG	2 cut(s)	268, 2364
NdeI	CATATG	1 cut(s)	1375
NdeII	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
NheI	GCTAGC	1 cut(s)	419
NlaIII	CATG	6 cut(s)	137, 158, 742, 1085, 2672, 3587
NlaIV	GGNNCC	9 cut(s)	548, 858, 1441, 1521, 2061, 2172, 2607, 2967, 3552
NmuCI	GTSAC	5 cut(s)	3, 1878, 2280, 3577, 3618
NsiI	ATGCAT	2 cut(s)	1267, 3240
NspI	RCATGY	1 cut(s)	3587
PaeR7I	CTCGAG	1 cut(s)	3153
PaqCI	CACCTGC	1 cut(s)	1246
PciI	ACATGT	1 cut(s)	3583
PciSI	GCTCTTC	1 cut(s)	1563
PcsI	WCGNNNNNNNCGW	1 cut(s)	766
PctI	GAATGC	1 cut(s)	185
PflFI	GACNNNGTC	1 cut(s)	2694
PflMI	CCANNNNNTGG	2 cut(s)	2393, 2438
PfoI	TCCNGGA	1 cut(s)	1446
PleI	GAGTC	3 cut(s)	164, 1238, 1837
PpsI	GAGTC	3 cut(s)	164, 1238, 1837
Ppu21I	YACGTR	2 cut(s)	73, 559
PpuMI	RGGWCCY	2 cut(s)	1487, 2309
PscI	ACATGT	1 cut(s)	3583
PshAI	GACNNNNGTC	1 cut(s)	3584
PshBI	ATTAAT	1 cut(s)	2802
Psp124BI	GAGCTC	1 cut(s)	1039
Psp5II	RGGWCCY	2 cut(s)	1487, 2309
Psp6I	CCWGG	2 cut(s)	1446, 2392
PspEI	GGTNACC	1 cut(s)	2280
PspFI	CCCAGC	2 cut(s)	2464, 2926
PspGI	CCWGG	2 cut(s)	1446, 2392
PspN4I	GGNNCC	9 cut(s)	548, 858, 1441, 1521, 2061, 2172, 2607, 2967, 3552
PspPI	GGNCC	5 cut(s)	1388, 1439, 1487, 2309, 2696
PspPPI	RGGWCCY	2 cut(s)	1487, 2309
PspXI	VCTCGAGB	1 cut(s)	3153
PstNI	CAGNNNCTG	1 cut(s)	2654
PsuI	RGATCY	6 cut(s)	856, 2781, 3038, 3158, 3295, 3550
PsyI	GACNNNGTC	1 cut(s)	2694
PvuII	CAGCTG	1 cut(s)	541
RsaI	GTAC	9 cut(s)	119, 275, 477, 548, 557, 1930, 2047, 2158, 3500
RsaNI	GTAC	9 cut(s)	118, 274, 476, 547, 556, 1929, 2046, 2157, 3499
RseI	CAYNNNNRTG	1 cut(s)	923
SacI	GAGCTC	1 cut(s)	1039
SalI	GTCGAC	1 cut(s)	2688
SapI	GCTCTTC	1 cut(s)	1563
Sau3AI	GATC	8 cut(s)	856, 1423, 2781, 2842, 3038, 3158, 3295, 3550
Sau96I	GGNCC	5 cut(s)	1388, 1439, 1487, 2309, 2696
ScaI	AGTACT	1 cut(s)	2047
SchI	GAGTC	3 cut(s)	164, 1238, 1837
ScrFI	CCNGG	2 cut(s)	1448, 2394
SfaNI	GCATC	7 cut(s)	915, 949, 1135, 1177, 1274, 1332, 1387
SfcI	CTRYAG	2 cut(s)	1818, 3165
Sfr274I	CTCGAG	1 cut(s)	3153
SinI	GGWCC	4 cut(s)	1439, 1487, 2309, 2696
SlaI	CTCGAG	1 cut(s)	3153
SmiMI	CAYNNNNRTG	1 cut(s)	923
SmlI	CTYRAG	1 cut(s)	3153
SmoI	CTYRAG	1 cut(s)	3153
SnaBI	TACGTA	1 cut(s)	73
SstI	GAGCTC	1 cut(s)	1039
StyD4I	CCNGG	2 cut(s)	1446, 2392
StyI	CCWWGG	4 cut(s)	743, 1311, 2152, 2600
TaiI	ACGT	4 cut(s)	75, 214, 561, 2695
TaqI	TCGA	5 cut(s)	51, 481, 2548, 2689, 3154
TatI	WGTACW	4 cut(s)	475, 1928, 2045, 3498
TauI	GCSGC	3 cut(s)	1151, 1732, 2654
TseFI	GTSAC	5 cut(s)	3, 1878, 2280, 3577, 3618
TseI	GCWGC	8 cut(s)	505, 541, 663, 2648, 2654, 3065, 3314, 3398
Tsp45I	GTSAC	5 cut(s)	3, 1878, 2280, 3577, 3618
TspGWI	ACGGA	2 cut(s)	872, 1905
Tth111I	GACNNNGTC	1 cut(s)	2694
Van91I	CCANNNNNTGG	2 cut(s)	2393, 2438
VneI	GTGCAC	1 cut(s)	2412
VpaK11BI	GGWCC	4 cut(s)	1439, 1487, 2309, 2696
VspI	ATTAAT	1 cut(s)	2802
XagI	CCTNNNNNAGG	2 cut(s)	704, 2304
XapI	RAATTY	6 cut(s)	53, 161, 681, 931, 2864, 3451
XceI	RCATGY	1 cut(s)	3587
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2884
XhoI	CTCGAG	1 cut(s)	3153
XmaJI	CCTAGG	1 cut(s)	743
XmiI	GTMKAC	2 cut(s)	69, 2689
ZrmI	AGTACT	1 cut(s)	2047
Zsp2I	ATGCAT	2 cut(s)	1267, 3240

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.