Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0015633

Physical Location & Seq

Genomic Coordinates Forward (+)

2982030 .. 2985337

Gene Structure 3308 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1024300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGGCAGAGAGTGCGGTCAACTTTGTGCTCGACAAGATCAAACCGTTTTTCGAAAATGGGGTTCAACTGCTCAGAGGGGTTCGAGCAGAACTTGTGTACCTCAAAGGGGAATTGGAGCGCATGAGAGCCTTACTGAGGGATGCCGATGCAATGGAAGAAAGTGACGAGGAGCTCAAAGTATGGGTTAAGCAAGTGCGAAATGTTGCTTATGACGCAGAAGATCTTCTAGATGAATTTGCGATTCTCCAAGCTTATAACAGTCAAAGCGTCCAAATGTACTGTCCATACTATAGGCTCTGTGGCTGTGTCAAGGACTTGAAAGCTCATTACCGGGTTGCTTGGGAGTTACAGAGCATCAACACCCGAATTCGAGATATTTTTGCAGTTTACAAAAGACTTCTTCCAAAGCTTAATGCTGTGAAAGGTTCAATATTTAACAATCCAGGTGACACATGGCATGACCGACGAGAGGATGCTCTTCTTTTAGAAAACACTGATGTTGTGGGCATACACAAGCGTAAAAAGAAACTTGTCAGGATGCTGGTCGAGGGTGGCTCTGGACTTGAAGTAGTTTCAGTCACTGGCATGGGAGGTATGGGGAAGACCACCTTGGTGAAGAAAGTCTACGATGATGATGAAGTGAAGAAACATTTCAAGCCCCGTGCTTGGATCACGGTTTCTCAATCTTTTCGGGTAGAGGATCTCCTTAAAGACATTATTCACAAACTCTTTGATGCAATCAGGAGGCCAGTTCCAGAAGGTGTGCACGACAAGAATAGCAATGAATTGAAAGCAATAATTAAGAAATTTCTGCAGAAAAGGAGGTACCTGATTGTTCTTGATGATGTATGGCACGCTGACAAATGGGAAACTGTCAAATATGCTCTGCCCAAAGGGAAGTTTGGCAGCAGAATCATGCTCACTACGCGTAATGCTGTATTCACCACTTGCTCGGAATCCAAAAGCAAGGTCTATCACTTGAAGCCCTTGCCTGCTGACGAGTCCTGGAAGCTGTTCATTAAGAAGGCATTTCTGGGGAAGCCATGTCCTCCTTATTTGGTGGAAAGATGTCAAGGTATCGTGAAAAGGTGTGAGGGCTTGCCCCTTGCAATTGTGGCAATAAGTGGGGTACTGGCTACAAAAGACAAGCGCAGGATAGATGAATGGGATTTCATTTGCCGCAGTCTTGGAGCTGAACTTCATGGCAATAACAAACTTGAAGATTTAAAGAAAGTACTTTCTCTGAGTTTTAATGATTTGCCCTATTATCTCAAGGCTTGCTTCCTATACTTGAGCCTCTTTCCCGAGGGTCATCTGATTCGGCGTATGAGACTGATTCGCTTATGGATAGCGGAAGGATTTGTCGAAGCTATAGAAGGAAGAACTTTGGAGGAAGTAGCTGAGGACTACCTCAAGGAGCTCTTGAACAGAAATTTGATCCTGGTGGGAGACACCACTAGTGATGGGAGGGTCAAAACTTATCGGCTTCATGGCCTTTTGCGGGAGATTATTATTTCAAAGTCAAGGGATCAAAACTTTGCAGCCATAGTCAAAGACCAGAACACAATATGGCCTGATAGAGTTCGTCGTCTGTCAATACATAATGCATTGGACACTGTACAAGAAAAAAGGTCAGTTCCTCAACTTCGTTCCTTGTTTATGTTTGGGGCGGTTGCTAGGTCATCCATGAGAAAGTATTTTGCCACTGGGTTTAGGCTGCTTGGGGTTTTAGATTTGGAAGCTGCACCTTTAAAGAAATTTCCAAGGGAAATCCTGGACCTGTTTTATTTAAAGTATCTAAGCTTGAGGAAAACCCAGGTTAAATTCATTCCAAGAAGCATAGGGAATCTTAGTAACCTGGAGACGTTGGATCTCAAAAAGACCAATGTCACTGAATTGCCTCTTGAGATACTGAAGCTCCAAAAACTTCGCCATCTCCTGGTTTATCGTCTTAAGAATGAATCTTATGCACACTTCTATTCCAAGTTTGGCTTCTCGGCGCTTTCAAACATAGGAGAATTGCAGTCCCTGCAAAAGCTCTGCTTTATAGAGGCAAATGATCAGAGTTGTGGGATGACAATGAGGGAGTTAGGTAAGCTTAAGCAATTGAGGAGGCTTGGAATTATGAAATTGAGAAAACAAGATGGATTGGCTTTGTGCTTGTCAATTGAAAGTTTAACCAAGCTGCGTGCGCTTTCTGTAACATCAACGGGAGAGAGTGAGGTTATTGATCTCCATCACATTTCTTCCCCTCCTCAGTTCCTCGAACGCCTATACTTGATAGGACGATTGGAAGAAATGCCGAACTGGATACCTTCACTTAACAGTTTGGCCAGACTGTTTTTGAAATGGAGTCAGTTGGAAGATGATCCACTCGTACATCTTCAAGACCTGCCCAACCTTATTCATCTTGAATTGCTCCATGCTTGTGATAGCGACATGTTATCCTTCAATGGAAGGGGATTCAAAAAGCTCAAGGTCTTGGGTCTCGACAAATTTGACAAGCTCCAATGCGTGAAGGTGGAGGAGGGAGCAATGCCATGCCTTGAGAAGCTAACTATCCAACGATGCAAGTCGATGAAGAGGGTACCCTCGGGAGTGAAGCACCTTACCAAACTGAAGTTACTTGAATTCTTTGAAATGCCGAATGAATTAATCTTGAAACTGCGACCAGATGGAGAAGGCGAAGATTATGAGGAAGTTCAACATATCCCAGATGTTTATTCAGCCTATTGGAGAGATGGGGGCTGGGATGTGTATTCAATAGAGAGCTTCGGGGAGATAGAAAATGCTCCTGTCCGGAGAAGACATGATCAGCTTCGTCCTCTATGGAAGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009605 GO:0009607 GO:0009626 GO:0009814 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0043207 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0098542 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

947

Amino Acids

109.27

Weight (kDa)

9.24

Isoelectric Point (pI)

43.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 85	6.5e-25	Rx N-terminal domain
NB-ARC	PF00931	172 - 345	1.8e-42	NB-ARC domain
WHD_DRP	PF23559	433 - 504	2.2e-20	Disease resistance protein Winged helix domain
LRR_14	PF23598	548 - 876	3.9e-46	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g23430 FvH4_5g23430 FvH4_5g23430
malus_domestica	MD06G1024300.v1.1
prunus_persica	Prupe.5G025300_v2.0.a1 Prupe.5G025300_v2.0.a1
pyrus_communis	pycom06g01970
rosa_chinensis	RchiOBHm_Chr7g0212491
rosa_multiflora	Rmu_sc0007576.1_g000013
rosa_roxburghii	Rroxscaffold_3G00246750
rosa_rugosa	Rorug07G0134600 Rorug07G0134800
rosa_samantha	Rh7BG261400 Rh7CG284200 Rh7DG274100
rosa_wichuraiana	Rw7G022880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	255
Acc36I	ACCTGC	1 cut(s)	2400
Acc65I	GGTACC	2 cut(s)	825, 2587
AccB1I	GGYRCC	2 cut(s)	825, 2587
AccB7I	CCANNNNNTGG	1 cut(s)	1939
AccI	GTMKAC	1 cut(s)	624
AccII	CGCG	1 cut(s)	928
AccIII	TCCGGA	1 cut(s)	2799
AciI	CCGC	5 cut(s)	14, 1180, 1352, 1501, 1670
AclWI	GGATC	6 cut(s)	677, 708, 1432, 1536, 1878, 2363
AcoI	YGGCCR	1 cut(s)	2331
AcsI	RAATTY	8 cut(s)	233, 366, 806, 1432, 1757, 1823, 2495, 2630
AcuI	CTGAAG	2 cut(s)	1934, 2639
AfaI	GTAC	8 cut(s)	98, 278, 827, 1131, 1236, 1620, 2379, 2589
AfiI	CCNNNNNNNGG	6 cut(s)	106, 135, 469, 666, 1501, 1939
AflII	CTTAAG	2 cut(s)	1952, 2099
AflIII	ACRYGT	2 cut(s)	926, 2439
AhlI	ACTAGT	1 cut(s)	1457
AjnI	CCWGG	7 cut(s)	442, 1004, 1440, 1773, 1815, 1857, 1938
AjuI	GAANNNNNNNTTGG	4 cut(s)	593, 625, 1976, 2008
AleI	CACNNNNGTG	1 cut(s)	611
Alw21I	GWGCWC	4 cut(s)	30, 174, 768, 1422
Alw26I	GTCTC	4 cut(s)	1324, 1443, 1856, 2492
Alw44I	GTGCAC	1 cut(s)	764
AlwI	GGATC	6 cut(s)	677, 708, 1432, 1536, 1878, 2363
AlwNI	CAGNNNCTG	2 cut(s)	581, 2029
Ama87I	CYCGRG	2 cut(s)	1304, 2593
Aor13HI	TCCGGA	1 cut(s)	2799
AoxI	GGCC	4 cut(s)	746, 1492, 1571, 2331
ApaLI	GTGCAC	1 cut(s)	764
ApeKI	GCWGC	5 cut(s)	906, 1541, 1717, 1742, 2185
ApoI	RAATTY	8 cut(s)	233, 366, 806, 1432, 1757, 1823, 2495, 2630
ArsI	GACNNNNNNTTYG	4 cut(s)	265, 297, 954, 986
AseI	ATTAAT	1 cut(s)	2654
Asp700I	GAANNNNTTC	3 cut(s)	222, 650, 1013
Asp718I	GGTACC	2 cut(s)	825, 2587
AspLEI	GCGC	4 cut(s)	120, 1152, 2002, 2194
AspS9I	GGNCC	1 cut(s)	1777
AsuC2I	CCSGG	1 cut(s)	332
AsuHPI	GGTGA	3 cut(s)	458, 625, 934
AsuII	TTCGAA	1 cut(s)	51
AvaI	CYCGRG	2 cut(s)	1304, 2593
AvaII	GGWCC	1 cut(s)	1777
BaeGI	GKGCMC	1 cut(s)	768
BaeI	ACNNNNGTAYC	2 cut(s)	80, 113
BalI	TGGCCA	1 cut(s)	2333
BanI	GGYRCC	2 cut(s)	825, 2587
BanII	GRGCYC	2 cut(s)	174, 1422
BarI	GAAGNNNNNNTAC	2 cut(s)	608, 640
BbsI	GAAGAC	2 cut(s)	608, 2812
Bbv12I	GWGCWC	4 cut(s)	30, 174, 768, 1422
BbvCI	CCTCAGC	1 cut(s)	1401
BbvI	GCAGC	5 cut(s)	918, 1553, 1704, 1729, 2172
BccI	CCATC	6 cut(s)	1457, 1941, 2138, 2244, 2669, 2735
BciT130I	CCWGG	7 cut(s)	444, 1006, 1442, 1775, 1817, 1859, 1940
BciVI	GTATCC	1 cut(s)	2304
BclI	TGATCA	2 cut(s)	2059, 2812
BcnI	CCSGG	1 cut(s)	332
BcoDI	GTCTC	4 cut(s)	1324, 1443, 1856, 2492
BcuI	ACTAGT	1 cut(s)	1457
BfaI	CTAG	3 cut(s)	227, 1458, 1677
BfmI	CTRYAG	3 cut(s)	289, 812, 1371
BfoI	RGCGCY	1 cut(s)	2003
BfrI	CTTAAG	2 cut(s)	1952, 2099
BfuAI	ACCTGC	1 cut(s)	2400
BfuI	GTATCC	1 cut(s)	2304
BglII	AGATCT	1 cut(s)	220
BisI	GCNGC	6 cut(s)	907, 1180, 1542, 1718, 1743, 2186
BlsI	GCNGC	6 cut(s)	908, 1181, 1543, 1719, 1744, 2187
BmcAI	AGTACT	1 cut(s)	1236
Bme1390I	CCNGG	8 cut(s)	332, 444, 1006, 1442, 1775, 1817, 1859, 1940
Bme18I	GGWCC	1 cut(s)	1777
BmeT110I	CYCGRG	2 cut(s)	1304, 2593
BmgT120I	GGNCC	1 cut(s)	1777
BmiI	GGNNCC	2 cut(s)	827, 2589
BmrFI	CCNGG	8 cut(s)	332, 444, 1006, 1442, 1775, 1817, 1859, 1940
BmrI	ACTGGG	1 cut(s)	1716
BmsI	GCATC	7 cut(s)	130, 136, 363, 463, 528, 724, 2558
BmuI	ACTGGG	1 cut(s)	1716
BoxI	GACNNNNGTC	1 cut(s)	1886
BpiI	GAAGAC	2 cut(s)	608, 2812
BplI	GAGNNNNNCTC	2 cut(s)	539, 571
BpmI	CTGGAG	1 cut(s)	1880
Bpu10I	CCTNAGC	1 cut(s)	1401
Bpu14I	TTCGAA	1 cut(s)	51
BpuEI	CTTGAG	7 cut(s)	1256, 1312, 1397, 1825, 1925, 2459, 2567
BpuMI	CCSGG	1 cut(s)	332
BsaBI	GATNNNNATC	1 cut(s)	2235
BsaI	GGTCTC	1 cut(s)	2492
BsaJI	CCNNGG	5 cut(s)	609, 1305, 1763, 1815, 2592
BsaWI	WCCGGW	1 cut(s)	2799
BsaXI	ACNNNNNCTCC	2 cut(s)	1902, 1932
Bsc4I	CCNNNNNNNGG	6 cut(s)	106, 135, 469, 666, 1501, 1939
Bse1I	ACTGG	5 cut(s)	586, 749, 1137, 1711, 2312
Bse3DI	GCAATG	3 cut(s)	156, 787, 2541
Bse8I	GATNNNNATC	1 cut(s)	2235
BseAI	TCCGGA	1 cut(s)	2799
BseBI	CCWGG	7 cut(s)	444, 1006, 1442, 1775, 1817, 1859, 1940
BseDI	CCNNGG	5 cut(s)	609, 1305, 1763, 1815, 2592
BseGI	GGATG	6 cut(s)	145, 478, 543, 1682, 2079, 2758
BseJI	GATNNNNATC	1 cut(s)	2235
BseLI	CCNNNNNNNGG	6 cut(s)	106, 135, 469, 666, 1501, 1939
BseMI	GCAATG	3 cut(s)	156, 787, 2541
BseMII	CTCAG	5 cut(s)	85, 125, 1235, 1392, 2270
BseNI	ACTGG	5 cut(s)	586, 749, 1137, 1711, 2312
BseRI	GAGGAG	4 cut(s)	182, 2125, 2244, 2540
BseSI	GKGCMC	1 cut(s)	768
BseXI	GCAGC	5 cut(s)	918, 1553, 1704, 1729, 2172
BseYI	CCCAGC	1 cut(s)	2748
BsgI	GTGCAG	1 cut(s)	1728
Bsh1236I	CGCG	1 cut(s)	928
BshFI	GGCC	4 cut(s)	748, 1494, 1573, 2333
BshNI	GGYRCC	2 cut(s)	825, 2587
BsiHKAI	GWGCWC	4 cut(s)	30, 174, 768, 1422
BsiHKCI	CYCGRG	2 cut(s)	1304, 2593
BsiSI	CCGG	2 cut(s)	331, 2800
BslFI	GGGAC	1 cut(s)	2011
BslI	CCNNNNNNNGG	6 cut(s)	106, 135, 469, 666, 1501, 1939
BsmAI	GTCTC	4 cut(s)	1324, 1443, 1856, 2492
BsmBI	CGTCTC	1 cut(s)	1856
BsmFI	GGGAC	1 cut(s)	2011
BsnI	GGCC	4 cut(s)	748, 1494, 1573, 2333
Bso31I	GGTCTC	1 cut(s)	2492
BsoBI	CYCGRG	2 cut(s)	1304, 2593
Bsp119I	TTCGAA	1 cut(s)	51
Bsp1286I	GDGCHC	4 cut(s)	30, 174, 768, 1422
Bsp13I	TCCGGA	1 cut(s)	2799
Bsp1407I	TGTACA	1 cut(s)	1618
BspACI	CCGC	5 cut(s)	14, 1180, 1352, 1501, 1670
BspANI	GGCC	4 cut(s)	748, 1494, 1573, 2333
BspCNI	CTCAG	5 cut(s)	84, 126, 1236, 1393, 2269
BspEI	TCCGGA	1 cut(s)	2799
BspFNI	CGCG	1 cut(s)	928
BspLI	GGNNCC	2 cut(s)	827, 2589
BspMAI	CTGCAG	1 cut(s)	816
BspMI	ACCTGC	1 cut(s)	2400
BspPI	GGATC	6 cut(s)	677, 708, 1432, 1536, 1878, 2363
BspQI	GCTCTTC	1 cut(s)	483
BspT104I	TTCGAA	1 cut(s)	51
BspT107I	GGYRCC	2 cut(s)	825, 2587
BspTI	CTTAAG	2 cut(s)	1952, 2099
BspTNI	GGTCTC	1 cut(s)	2492
BsrDI	GCAATG	3 cut(s)	156, 787, 2541
BsrGI	TGTACA	1 cut(s)	1618
BsrI	ACTGG	5 cut(s)	586, 749, 1137, 1711, 2312
BssECI	CCNNGG	5 cut(s)	609, 1305, 1763, 1815, 2592
BssT1I	CCWWGG	2 cut(s)	609, 1763
Bst2UI	CCWGG	7 cut(s)	444, 1006, 1442, 1775, 1817, 1859, 1940
Bst4CI	ACNGT	8 cut(s)	45, 260, 281, 676, 874, 1618, 2327, 2340
Bst6I	CTCTTC	2 cut(s)	483, 2576
BstAFI	CTTAAG	2 cut(s)	1952, 2099
BstAPI	GCANNNNNTGC	2 cut(s)	11, 2029
BstAUI	TGTACA	1 cut(s)	1618
BstBI	TTCGAA	1 cut(s)	51
BstC8I	GCNNGC	5 cut(s)	855, 993, 1100, 1279, 2190
BstDEI	CTNAG	7 cut(s)	71, 134, 1244, 1401, 1799, 1850, 2256
BstENI	CCTNNNNNAGG	1 cut(s)	133
BstF5I	GGATG	6 cut(s)	145, 478, 543, 1682, 2079, 2758
BstFNI	CGCG	1 cut(s)	928
BstH2I	RGCGCY	1 cut(s)	2003
BstHHI	GCGC	4 cut(s)	120, 1152, 2002, 2194
BstMAI	GTCTC	4 cut(s)	1324, 1443, 1856, 2492
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 212, 925, 1115, 2029, 2191
BstNI	CCWGG	7 cut(s)	444, 1006, 1442, 1775, 1817, 1859, 1940
BstNSI	RCATGY	1 cut(s)	2443
BstPAI	GACNNNNGTC	1 cut(s)	1886
BstSCI	CCNGG	8 cut(s)	330, 442, 1004, 1440, 1773, 1815, 1857, 1938
BstSFI	CTRYAG	3 cut(s)	289, 812, 1371
BstSLI	GKGCMC	1 cut(s)	768
BstUI	CGCG	1 cut(s)	928
BstV1I	GCAGC	5 cut(s)	918, 1553, 1704, 1729, 2172
BstV2I	GAAGAC	2 cut(s)	608, 2812
BstX2I	RGATCY	3 cut(s)	220, 700, 1870
BstYI	RGATCY	3 cut(s)	220, 700, 1870
BsuI	GTATCC	1 cut(s)	2304
BsuRI	GGCC	4 cut(s)	748, 1494, 1573, 2333
BtsCI	GGATG	6 cut(s)	145, 478, 543, 1682, 2079, 2758
BtsIMutI	CAGTG	5 cut(s)	492, 579, 1614, 1704, 1890
BveI	ACCTGC	1 cut(s)	2400
Cac8I	GCNNGC	5 cut(s)	855, 993, 1100, 1279, 2190
CaiI	CAGNNNCTG	2 cut(s)	581, 2029
CfoI	GCGC	4 cut(s)	120, 1152, 2002, 2194
Cfr13I	GGNCC	1 cut(s)	1777
CseI	GACGC	2 cut(s)	221, 256
Csp6I	GTAC	8 cut(s)	97, 277, 826, 1130, 1235, 1619, 2378, 2588
CviQI	GTAC	8 cut(s)	97, 277, 826, 1130, 1235, 1619, 2378, 2588
DdeI	CTNAG	7 cut(s)	71, 134, 1244, 1401, 1799, 1850, 2256
DraI	TTTAAA	3 cut(s)	1227, 1752, 1791
EaeI	YGGCCR	1 cut(s)	2331
Eam1104I	CTCTTC	2 cut(s)	483, 2576
EarI	CTCTTC	2 cut(s)	483, 2576
Ecl136II	GAGCTC	2 cut(s)	172, 1420
Eco130I	CCWWGG	2 cut(s)	609, 1763
Eco24I	GRGCYC	2 cut(s)	174, 1422
Eco31I	GGTCTC	1 cut(s)	2492
Eco47I	GGWCC	1 cut(s)	1777
Eco53kI	GAGCTC	2 cut(s)	172, 1420
Eco57I	CTGAAG	2 cut(s)	1934, 2639
Eco88I	CYCGRG	2 cut(s)	1304, 2593
EcoICRI	GAGCTC	2 cut(s)	172, 1420
EcoNI	CCTNNNNNAGG	1 cut(s)	133
EcoRI	GAATTC	2 cut(s)	366, 2630
EcoRII	CCWGG	7 cut(s)	442, 1004, 1440, 1773, 1815, 1857, 1938
EcoT14I	CCWWGG	2 cut(s)	609, 1763
EcoT22I	ATGCAT	1 cut(s)	1609
EcoT38I	GRGCYC	2 cut(s)	174, 1422
ErhI	CCWWGG	2 cut(s)	609, 1763
Esp3I	CGTCTC	1 cut(s)	1856
FaqI	GGGAC	1 cut(s)	2011
FauI	CCCGC	1 cut(s)	1494
FbaI	TGATCA	2 cut(s)	2059, 2812
FblI	GTMKAC	1 cut(s)	624
Fnu4HI	GCNGC	6 cut(s)	907, 1180, 1542, 1718, 1743, 2186
FokI	GGATG	6 cut(s)	152, 485, 550, 1669, 2086, 2765
FriOI	GRGCYC	2 cut(s)	174, 1422
Fsp4HI	GCNGC	6 cut(s)	907, 1180, 1542, 1718, 1743, 2186
FspBI	CTAG	3 cut(s)	227, 1458, 1677
GlaI	GCGC	4 cut(s)	119, 1151, 2001, 2193
GluI	GCNGC	6 cut(s)	907, 1180, 1542, 1718, 1743, 2186
GsaI	CCCAGC	1 cut(s)	2752
GsuI	CTGGAG	1 cut(s)	1880
HaeII	RGCGCY	1 cut(s)	2003
HaeIII	GGCC	4 cut(s)	748, 1494, 1573, 2333
HapII	CCGG	2 cut(s)	331, 2800
HgaI	GACGC	2 cut(s)	221, 256
HhaI	GCGC	4 cut(s)	120, 1152, 2002, 2194
Hin6I	GCGC	4 cut(s)	118, 1150, 2000, 2192
HinP1I	GCGC	4 cut(s)	118, 1150, 2000, 2192
HincII	GTYRAC	1 cut(s)	19
HindII	GTYRAC	1 cut(s)	19
HindIII	AAGCTT	4 cut(s)	249, 407, 1801, 2096
HpaII	CCGG	2 cut(s)	331, 2800
HphI	GGTGA	3 cut(s)	458, 625, 934
Hpy166II	GTNNAC	5 cut(s)	19, 97, 388, 625, 766
Hpy188I	TCNGA	5 cut(s)	74, 955, 1245, 1317, 2064
Hpy8I	GTNNAC	5 cut(s)	19, 97, 388, 625, 766
Hpy99I	CGWCG	2 cut(s)	468, 1590
HpyCH4III	ACNGT	8 cut(s)	45, 260, 281, 676, 874, 1618, 2327, 2340
HpyCH4IV	ACGT	1 cut(s)	1865
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 212, 925, 1115, 2029, 2191
HpyF3I	CTNAG	7 cut(s)	71, 134, 1244, 1401, 1799, 1850, 2256
HpySE526I	ACGT	1 cut(s)	1865
HspAI	GCGC	4 cut(s)	118, 1150, 2000, 2192
Kpn2I	TCCGGA	1 cut(s)	2799
KpnI	GGTACC	2 cut(s)	829, 2591
Ksp22I	TGATCA	2 cut(s)	2059, 2812
LguI	GCTCTTC	1 cut(s)	483
LmnI	GCTCC	9 cut(s)	115, 169, 1190, 1417, 1923, 2424, 2511, 2531, 2797
Lsp1109I	GCAGC	5 cut(s)	918, 1553, 1704, 1729, 2172
LweI	GCATC	7 cut(s)	130, 136, 363, 463, 528, 724, 2558
MaeI	CTAG	3 cut(s)	227, 1458, 1677
MaeII	ACGT	1 cut(s)	1865
MaeIII	GTNAC	8 cut(s)	161, 345, 446, 577, 1853, 1888, 2200, 2622
MfeI	CAATTG	3 cut(s)	1110, 2105, 2166
MflI	RGATCY	3 cut(s)	220, 700, 1870
MhlI	GDGCHC	4 cut(s)	30, 174, 768, 1422
MlsI	TGGCCA	1 cut(s)	2333
MluI	ACGCGT	1 cut(s)	926
MluNI	TGGCCA	1 cut(s)	2333
MlyI	GAGTC	2 cut(s)	1010, 2362
MmeI	TCCRAC	3 cut(s)	1848, 2340, 2587
Mox20I	TGGCCA	1 cut(s)	2333
Mph1103I	ATGCAT	1 cut(s)	1609
MroI	TCCGGA	1 cut(s)	2799
MroXI	GAANNNNTTC	3 cut(s)	222, 650, 1013
MscI	TGGCCA	1 cut(s)	2333
MslI	CAYNNNNRTG	3 cut(s)	584, 611, 2427
Msp20I	TGGCCA	1 cut(s)	2333
MspCI	CTTAAG	2 cut(s)	1952, 2099
MspI	CCGG	2 cut(s)	331, 2800
MspR9I	CCNGG	8 cut(s)	332, 444, 1006, 1442, 1775, 1817, 1859, 1940
MunI	CAATTG	3 cut(s)	1110, 2105, 2166
MvaI	CCWGG	7 cut(s)	444, 1006, 1442, 1775, 1817, 1859, 1940
MvnI	CGCG	1 cut(s)	928
MwoI	GCNNNNNNNGC	6 cut(s)	11, 212, 925, 1115, 2029, 2191
NciI	CCSGG	1 cut(s)	332
NlaIV	GGNNCC	2 cut(s)	827, 2589
NmeAIII	GCCGAG	1 cut(s)	1976
NmuCI	GTSAC	4 cut(s)	161, 446, 577, 1888
NsiI	ATGCAT	1 cut(s)	1609
NspI	RCATGY	1 cut(s)	2443
NspV	TTCGAA	1 cut(s)	51
OliI	CACNNNNGTG	1 cut(s)	611
PciI	ACATGT	1 cut(s)	2439
PciSI	GCTCTTC	1 cut(s)	483
PdmI	GAANNNNTTC	3 cut(s)	222, 650, 1013
PfeI	GAWTC	8 cut(s)	241, 912, 956, 1318, 1336, 1846, 1961, 2463
PflMI	CCANNNNNTGG	1 cut(s)	1939
PfoI	TCCNGGA	2 cut(s)	1004, 1773
PkrI	GCNGC	6 cut(s)	908, 1181, 1543, 1719, 1744, 2187
PleI	GAGTC	2 cut(s)	1009, 2361
PpsI	GAGTC	2 cut(s)	1009, 2361
PscI	ACATGT	1 cut(s)	2439
PshAI	GACNNNNGTC	1 cut(s)	1886
PshBI	ATTAAT	1 cut(s)	2654
PsiI	TTATAA	1 cut(s)	255
Psp124BI	GAGCTC	2 cut(s)	174, 1422
Psp6I	CCWGG	7 cut(s)	442, 1004, 1440, 1773, 1815, 1857, 1938
PspFI	CCCAGC	1 cut(s)	2748
PspGI	CCWGG	7 cut(s)	442, 1004, 1440, 1773, 1815, 1857, 1938
PspN4I	GGNNCC	2 cut(s)	827, 2589
PspPI	GGNCC	1 cut(s)	1777
PstI	CTGCAG	1 cut(s)	816
PstNI	CAGNNNCTG	2 cut(s)	581, 2029
PsuI	RGATCY	3 cut(s)	220, 700, 1870
RsaI	GTAC	8 cut(s)	98, 278, 827, 1131, 1236, 1620, 2379, 2589
RsaNI	GTAC	8 cut(s)	97, 277, 826, 1130, 1235, 1619, 2378, 2588
RseI	CAYNNNNRTG	3 cut(s)	584, 611, 2427
SacI	GAGCTC	2 cut(s)	174, 1422
SapI	GCTCTTC	1 cut(s)	483
SatI	GCNGC	6 cut(s)	907, 1180, 1542, 1718, 1743, 2186
Sau96I	GGNCC	1 cut(s)	1777
ScaI	AGTACT	1 cut(s)	1236
SchI	GAGTC	2 cut(s)	1010, 2362
ScrFI	CCNGG	8 cut(s)	332, 444, 1006, 1442, 1775, 1817, 1859, 1940
SduI	GDGCHC	4 cut(s)	30, 174, 768, 1422
SfaNI	GCATC	7 cut(s)	130, 136, 363, 463, 528, 724, 2558
SfcI	CTRYAG	3 cut(s)	289, 812, 1371
SfuI	TTCGAA	1 cut(s)	51
SinI	GGWCC	1 cut(s)	1777
SmiMI	CAYNNNNRTG	3 cut(s)	584, 611, 2427
SmlI	CTYRAG	9 cut(s)	1271, 1291, 1412, 1804, 1904, 1952, 2099, 2474, 2546
SmoI	CTYRAG	9 cut(s)	1271, 1291, 1412, 1804, 1904, 1952, 2099, 2474, 2546
SpeI	ACTAGT	1 cut(s)	1457
SsiI	CCGC	5 cut(s)	14, 1180, 1352, 1501, 1670
SspI	AATATT	1 cut(s)	432
SspMI	CTAG	3 cut(s)	227, 1458, 1677
SstI	GAGCTC	2 cut(s)	174, 1422
StyD4I	CCNGG	8 cut(s)	330, 442, 1004, 1440, 1773, 1815, 1857, 1938
StyI	CCWWGG	2 cut(s)	609, 1763
TaaI	ACNGT	8 cut(s)	45, 260, 281, 676, 874, 1618, 2327, 2340
TaiI	ACGT	1 cut(s)	1868
TaqI	TCGA	9 cut(s)	30, 51, 82, 370, 546, 1365, 2265, 2490, 2576
TaqII	GACCGA	1 cut(s)	477
TatI	WGTACW	3 cut(s)	276, 1234, 1618
TauI	GCSGC	1 cut(s)	1182
TfiI	GAWTC	8 cut(s)	241, 912, 956, 1318, 1336, 1846, 1961, 2463
TscAI	CASTG	5 cut(s)	499, 586, 1621, 1711, 1897
TseFI	GTSAC	4 cut(s)	161, 446, 577, 1888
TseI	GCWGC	5 cut(s)	906, 1541, 1717, 1742, 2185
Tsp45I	GTSAC	4 cut(s)	161, 446, 577, 1888
TspRI	CASTG	5 cut(s)	499, 586, 1621, 1711, 1897
Van91I	CCANNNNNTGG	1 cut(s)	1939
Vha464I	CTTAAG	2 cut(s)	1952, 2099
VneI	GTGCAC	1 cut(s)	764
VpaK11BI	GGWCC	1 cut(s)	1777
VspI	ATTAAT	1 cut(s)	2654
XagI	CCTNNNNNAGG	1 cut(s)	133
XapI	RAATTY	8 cut(s)	233, 366, 806, 1432, 1757, 1823, 2495, 2630
XbaI	TCTAGA	1 cut(s)	226
XceI	RCATGY	1 cut(s)	2443
XcmI	CCANNNNNNNNNTGG	1 cut(s)	450
XmiI	GTMKAC	1 cut(s)	624
XmnI	GAANNNNTTC	3 cut(s)	222, 650, 1013
XspI	CTAG	3 cut(s)	227, 1458, 1677
ZrmI	AGTACT	1 cut(s)	1236
Zsp2I	ATGCAT	1 cut(s)	1609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.