Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010771

Physical Location & Seq

Genomic Coordinates Reverse (-)

22947488 .. 22952317

Gene Structure 4830 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1095800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGTTAACAGAAGAGGTCAATGTAGGGAAAATGCCCTTTTCTTGTATAAGAGCTTTGGTTTGCTTTTCGGTGCTTTGAGCATTCCCCCTCTTTATGTTTATAAATGTGCATTTTCTGGAGGATTGCTCAATTACCAAACCGACGATGCGATATTTGGGGTGTTTTCAGTGATTTTTTGGACTCTTACTCTTATTTCTTTGCTAAAGTATGTCCTTGTCATGTTGAGCGCCGACGACAATGGTCAAGGTGGTGTTATTGCATTATACGCGCTCCTTTGCAGAAATGCGAAATTCTGTTTGCTGCCTAATTATCAGGCATCTGATGAGGAGATCTCCACATACCGCTACCCCGGTTGTTCCAATACGAACAGGCCCACTTCTCCATTGAAAAGGTTTATCGAGAGACATAAAAGTGCAAAAACCTGTCTACTTCTTGTAGTTTTATTTGGAGCTTCCATGGTGATTTGTGTTGGTATCCTCACTCCTGTAATATCAATTTTTGCATCTGTGGAGGGGCTACAATTTCAAGCAACGAATTTGCATAGTGGTGTGGTGGTTCTTATTGCCTGTATTTTATTGCTTGCCCTTTTTGTTTTGCAACACCGTGGCATCCACAAATTGGCCTTCATTTTCACTCCAATTATGATCCTTTGGCTACTGTTAATTGCTGCTGTTGGAATCTACAATATCATCGAGTGGAATCCAAGGGTATATCAGGCTCTTTCTCCGTATTACATTTACATATTCTTTAAGACGACAGGAAAAGATGGCTGGCTTTCTCTTGGAGGAATACTTTTATGTGTGACTGGAACTGAAGCCATCTTTGCGGATCTTGGCCACTTCAGAACAGCACCAGTAAGGGTTGCATTTTCTTTTGTGATTTACCCATGCCTAATACTTCAATACATGGGACAAGCTGCGTTTCTTTCAAGAAATCTATCTGCAGTATCTATGAGCTTTTTCGCTTCTATTCCACCTCCCTTGTTGTGGCCAGTACTTGTGGTTGCACCTTTGGCTGCCATTGTTGCCAGTCAACCTGTTATCTCTTCCACATTCTCAGTTGTCAAGCAATGTCAGGCAATACGATGTTTCCCTCGCGTCAAGGTTGTACATACAAAGAGACGGATATCTGGTCAGATATACATCCCTGAGTTGAACTGGTTTCTTATGATCATCAGTCTGGCTGTCACAATTGGTTTTCGAGACACAATTCGTATAGCAAATGCTTATGGGATTGCATTTTTGGGTGTTACAATCATAACGACATGGTTGGTATCGCTAGTCATCGATCTTGTTTGGCATCAGAGTTTCATGCTTTCCCTTTTATTTTGTGTACTTTTCGGTTCAGTTGAAGCCATCTACCTTTCATCTTTGTGCGTGAGAATCCTCAAAGGTACATGGCTTCCCCTGGTTTTGTCTGCTGTCTTCTTGGTAGTTATGTATGTCTGGCAATATGGCACGCAGAAGAAGTATTTGTATGACTTGCATAACAAGGTTTCAATGAAGTGGATGCTCACGCTGGGTCCTAGTCTTGGCATTGTTAGGGTCCCTGGAATTGGCCTCATCTACACTGAATTGTCTACCGGAGTCCCAGCGTCATTTACTCATTTCTTAACCAACCTACCGGCTTTTTACCAAGTGATTGTCTTCGTTTGCGTTAAAACTGTTCCTGTTCCTTATGTTCCCCAAAAAGAAAGGTATCTTGTTGGCCGTGTTGGACCCAAATCTTTTGGGATGTACCGTTGTATGGTTCGGAATGGGTACAAAGACGTCTTTAAAAATGGGGACGACTTTGAAATTGATGTGGTGATGAGCATAGCAGAGTTCATCCAAATGGAAGCAGAAGGTTCTGGAACCCCTGAAGGCACTGTGGACCGCCGTATGGCAGTTGTGAAGACATCTGGGAAGTTTGGCACGGTGTTGGTTATGGCACAAACTTCTGGCCTTGGAGAAAGCAGCAGTTCAAGTTCTTCAGCGATTGTGAGCAGTGCCAAGTCTCCCACGCTGCAGAACTTGCAGGCCAACTATGAGCAAGAAGCACCAAAACTCAACTATTGGAGGCGGGCTCGGTTTGGATTGCTGGATGCAAAATGCAAAGACTCCCGCCTAAAGGAACAGCTCCAGGAACTTGTGGAAGCAAAGCATGCCGGGGTAGCATATGTAATAGGTCAGGCATACTTAAAGGCGAAATGGAATTCATCGTTCTTAAAGAAGTGCGCTATTGATGTTGCCTACTCTTTCTTACGCAAGAATTGCCGCTCTCCCGCTGTTACTCTGAACATCCCTCATATTTGTTTGATCAAGGTGGGCATGAACTACATCGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0031090 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

776

Amino Acids

86.19

Weight (kDa)

9.4

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	16 - 505	9.1e-144	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	517 - 773	3.8e-22	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010771)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23640
fragaria_vesca	FvH4_5g03590 FvH4_5g03590 FvH4_5g03590 FvH4_5g03590
malus_domestica	MD06G1095800.v1.1 MD14G1115800.v1.1 MD14G1115900.v1.1
prunus_persica	Prupe.5G110400_v2.0.a1
pyrus_communis	pycom06g09210
rosa_chinensis	RchiOBHm_Chr7g0196121
rosa_laevigata	RLG00000004062
rosa_multiflora	Rmu_sc0003017.1_g000008
rosa_roxburghii	Rroxscaffold_3G00259760
rosa_rugosa	Rorug07G0030800
rosa_samantha	Rh7AG156700 Rh7BG158400 Rh7CG162200 Rh7DG157500
rosa_wichuraiana	Rw7G013380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	102
AatII	GACGTC	1 cut(s)	1773
AccB7I	CCANNNNNTGG	1 cut(s)	619
AccBSI	CCGCTC	1 cut(s)	2259
AccI	GTMKAC	2 cut(s)	427, 1580
AccII	CGCG	2 cut(s)	269, 1098
AciI	CCGC	7 cut(s)	343, 827, 1876, 2062, 2104, 2257, 2265
AclWI	GGATC	2 cut(s)	640, 837
AcoI	YGGCCR	3 cut(s)	835, 989, 1708
AcsI	RAATTY	3 cut(s)	290, 535, 2194
AcuI	CTGAAG	4 cut(s)	826, 834, 1881, 1956
AcyI	GRCGYC	1 cut(s)	1770
AfaI	GTAC	6 cut(s)	996, 1110, 1335, 1396, 1739, 1763
AfiI	CCNNNNNNNGG	7 cut(s)	619, 987, 1532, 1556, 1747, 1882, 2110
AgsI	TTSAA	9 cut(s)	388, 527, 902, 930, 1156, 1352, 1500, 1796, 1965
AjnI	CCWGG	3 cut(s)	1407, 1549, 2121
AluBI	AGCT	5 cut(s)	54, 452, 917, 957, 2119
AluI	AGCT	5 cut(s)	54, 452, 917, 957, 2119
Alw26I	GTCTC	4 cut(s)	397, 1114, 1197, 2001
AlwI	GGATC	2 cut(s)	640, 837
AoxI	GGCC	8 cut(s)	371, 621, 835, 989, 1558, 1708, 1942, 2019
ApeKI	GCWGC	6 cut(s)	301, 668, 917, 1016, 1956, 2005
ApoI	RAATTY	3 cut(s)	290, 535, 2194
AspLEI	GCGC	3 cut(s)	230, 271, 2219
AspS9I	GGNCC	5 cut(s)	372, 1523, 1546, 1718, 1873
AsuC2I	CCSGG	2 cut(s)	351, 2149
AsuHPI	GGTGA	2 cut(s)	472, 1819
AvaII	GGWCC	4 cut(s)	1523, 1546, 1718, 1873
BalI	TGGCCA	2 cut(s)	837, 991
BanII	GRGCYC	1 cut(s)	2068
BbsI	GAAGAC	3 cut(s)	1417, 1639, 1901
BbvI	GCAGC	6 cut(s)	288, 655, 904, 1003, 1968, 1992
BccI	CCATC	3 cut(s)	761, 827, 1364
BceAI	ACGGC	2 cut(s)	1695, 1863
BciT130I	CCWGG	3 cut(s)	1409, 1551, 2123
BciVI	GTATCC	1 cut(s)	485
BclI	TGATCA	2 cut(s)	1170, 2298
BcnI	CCSGG	2 cut(s)	351, 2149
BcoDI	GTCTC	4 cut(s)	397, 1114, 1197, 2001
BfaI	CTAG	2 cut(s)	1280, 1527
BfmI	CTRYAG	2 cut(s)	942, 2006
BfoI	RGCGCY	1 cut(s)	231
BfuI	GTATCC	1 cut(s)	485
BglII	AGATCT	1 cut(s)	330
BisI	GCNGC	7 cut(s)	302, 669, 918, 1017, 1957, 2006, 2257
BlsI	GCNGC	7 cut(s)	303, 670, 919, 1018, 1958, 2007, 2258
BmcAI	AGTACT	1 cut(s)	996
Bme1390I	CCNGG	5 cut(s)	351, 1409, 1551, 2123, 2149
Bme18I	GGWCC	4 cut(s)	1523, 1546, 1718, 1873
BmgT120I	GGNCC	5 cut(s)	372, 1523, 1546, 1718, 1873
BmiI	GGNNCC	5 cut(s)	1524, 1547, 1548, 1720, 1855
BmrFI	CCNGG	5 cut(s)	351, 1409, 1551, 2123, 2149
BmsI	GCATC	7 cut(s)	136, 326, 512, 618, 1309, 1500, 2074
BoxI	GACNNNNGTC	1 cut(s)	240
BpiI	GAAGAC	3 cut(s)	1417, 1639, 1901
BplI	GAGNNNNNCTC	6 cut(s)	111, 143, 317, 349, 2050, 2082
BpmI	CTGGAG	2 cut(s)	138, 2105
BpuMI	CCSGG	2 cut(s)	351, 2149
Bsa29I	ATCGAT	1 cut(s)	1287
BsaBI	GATNNNNATC	1 cut(s)	1142
BsaHI	GRCGYC	1 cut(s)	1770
BsaJI	CCNNGG	8 cut(s)	349, 456, 604, 704, 1407, 1549, 1945, 2148
BsaWI	WCCGGW	1 cut(s)	1583
Bsc4I	CCNNNNNNNGG	7 cut(s)	619, 987, 1532, 1556, 1747, 1882, 2110
Bse118I	RCCGGY	1 cut(s)	1624
Bse1I	ACTGG	5 cut(s)	811, 854, 992, 1029, 1163
Bse3DI	GCAATG	1 cut(s)	1076
Bse8I	GATNNNNATC	1 cut(s)	1142
BseBI	CCWGG	3 cut(s)	1409, 1551, 2123
BseCI	ATCGAT	1 cut(s)	1287
BseDI	CCNNGG	8 cut(s)	349, 456, 604, 704, 1407, 1549, 1945, 2148
BseGI	GGATG	7 cut(s)	609, 1143, 1515, 1740, 1827, 2089, 2280
BseJI	GATNNNNATC	1 cut(s)	1142
BseLI	CCNNNNNNNGG	7 cut(s)	619, 987, 1532, 1556, 1747, 1882, 2110
BseMI	GCAATG	1 cut(s)	1076
BseMII	CTCAG	2 cut(s)	1071, 1140
BseNI	ACTGG	5 cut(s)	811, 854, 992, 1029, 1163
BseRI	GAGGAG	1 cut(s)	341
BseXI	GCAGC	6 cut(s)	288, 655, 904, 1003, 1968, 1992
BseYI	CCCAGC	2 cut(s)	1519, 1591
Bsh1236I	CGCG	2 cut(s)	269, 1098
BshFI	GGCC	8 cut(s)	373, 623, 837, 991, 1560, 1710, 1944, 2021
BshVI	ATCGAT	1 cut(s)	1287
BsiSI	CCGG	4 cut(s)	351, 1584, 1625, 2148
BslFI	GGGAC	4 cut(s)	924, 1532, 1574, 1799
BslI	CCNNNNNNNGG	7 cut(s)	619, 987, 1532, 1556, 1747, 1882, 2110
BsmAI	GTCTC	4 cut(s)	397, 1114, 1197, 2001
BsmBI	CGTCTC	1 cut(s)	1114
BsmFI	GGGAC	4 cut(s)	924, 1532, 1574, 1799
BsmI	GAATGC	1 cut(s)	81
BsnI	GGCC	8 cut(s)	373, 623, 837, 991, 1560, 1710, 1944, 2021
Bsp1286I	GDGCHC	1 cut(s)	2068
Bsp1407I	TGTACA	1 cut(s)	1108
Bsp143I	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
Bsp19I	CCATGG	1 cut(s)	456
BspACI	CCGC	7 cut(s)	343, 827, 1876, 2062, 2104, 2257, 2265
BspANI	GGCC	8 cut(s)	373, 623, 837, 991, 1560, 1710, 1944, 2021
BspCNI	CTCAG	2 cut(s)	1070, 1141
BspDI	ATCGAT	1 cut(s)	1287
BspFNI	CGCG	2 cut(s)	269, 1098
BspLI	GGNNCC	5 cut(s)	1524, 1547, 1548, 1720, 1855
BspMAI	CTGCAG	2 cut(s)	946, 2010
BspPI	GGATC	2 cut(s)	640, 837
BsrBI	CCGCTC	1 cut(s)	2259
BsrDI	GCAATG	1 cut(s)	1076
BsrFI	RCCGGY	1 cut(s)	1624
BsrGI	TGTACA	1 cut(s)	1108
BsrI	ACTGG	5 cut(s)	811, 854, 992, 1029, 1163
BssAI	RCCGGY	1 cut(s)	1624
BssECI	CCNNGG	8 cut(s)	349, 456, 604, 704, 1407, 1549, 1945, 2148
BssMI	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
BssNI	GRCGYC	1 cut(s)	1770
BssT1I	CCWWGG	3 cut(s)	456, 704, 1945
Bst2UI	CCWGG	3 cut(s)	1409, 1551, 2123
Bst4CI	ACNGT	6 cut(s)	605, 660, 1666, 1742, 1870, 1918
Bst6I	CTCTTC	2 cut(s)	7, 1051
BstACI	GRCGYC	1 cut(s)	1770
BstAPI	GCANNNNNTGC	3 cut(s)	2014, 2144, 2253
BstAUI	TGTACA	1 cut(s)	1108
BstC8I	GCNNGC	6 cut(s)	582, 773, 1460, 2019, 2064, 2145
BstDEI	CTNAG	2 cut(s)	1057, 1149
BstDSI	CCRYGG	2 cut(s)	456, 604
BstF5I	GGATG	7 cut(s)	609, 1143, 1515, 1740, 1827, 2089, 2280
BstFNI	CGCG	2 cut(s)	269, 1098
BstH2I	RGCGCY	1 cut(s)	231
BstHHI	GCGC	3 cut(s)	230, 271, 2219
BstKTI	GATC	6 cut(s)	333, 648, 832, 1173, 1291, 2301
BstMAI	GTCTC	4 cut(s)	397, 1114, 1197, 2001
BstMBI	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
BstMWI	GCNNNNNNNGC	8 cut(s)	266, 824, 1013, 1025, 2014, 2144, 2153, 2253
BstNI	CCWGG	3 cut(s)	1409, 1551, 2123
BstNSI	RCATGY	1 cut(s)	2147
BstPAI	GACNNNNGTC	1 cut(s)	240
BstSCI	CCNGG	5 cut(s)	349, 1407, 1549, 2121, 2147
BstSFI	CTRYAG	2 cut(s)	942, 2006
BstUI	CGCG	2 cut(s)	269, 1098
BstV1I	GCAGC	6 cut(s)	288, 655, 904, 1003, 1968, 1992
BstV2I	GAAGAC	3 cut(s)	1417, 1639, 1901
BstX2I	RGATCY	2 cut(s)	330, 829
BstYI	RGATCY	2 cut(s)	330, 829
Bsu15I	ATCGAT	1 cut(s)	1287
BsuI	GTATCC	1 cut(s)	485
BsuRI	GGCC	8 cut(s)	373, 623, 837, 991, 1560, 1710, 1944, 2021
BsuTUI	ATCGAT	1 cut(s)	1287
BtgI	CCRYGG	2 cut(s)	456, 604
BtsCI	GGATG	7 cut(s)	609, 1143, 1515, 1740, 1827, 2089, 2280
BtsI	GCAGTG	1 cut(s)	1993
BtsIMutI	CAGTG	4 cut(s)	174, 1569, 1866, 1993
Cac8I	GCNNGC	6 cut(s)	582, 773, 1460, 2019, 2064, 2145
CfoI	GCGC	3 cut(s)	230, 271, 2219
Cfr10I	RCCGGY	1 cut(s)	1624
Cfr13I	GGNCC	5 cut(s)	372, 1523, 1546, 1718, 1873
ClaI	ATCGAT	1 cut(s)	1287
CseI	GACGC	2 cut(s)	1087, 1584
Csp6I	GTAC	6 cut(s)	995, 1109, 1334, 1395, 1738, 1762
CspCI	CAANNNNNGTGG	2 cut(s)	963, 998
CviAII	CATG	9 cut(s)	220, 457, 888, 907, 1266, 1312, 1398, 2144, 2311
CviQI	GTAC	6 cut(s)	995, 1109, 1334, 1395, 1738, 1762
DdeI	CTNAG	2 cut(s)	1057, 1149
DpnI	GATC	6 cut(s)	332, 647, 831, 1172, 1290, 2300
DpnII	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
DraI	TTTAAA	1 cut(s)	1777
EaeI	YGGCCR	3 cut(s)	835, 989, 1708
Eam1104I	CTCTTC	2 cut(s)	7, 1051
EarI	CTCTTC	2 cut(s)	7, 1051
Eco130I	CCWWGG	3 cut(s)	456, 704, 1945
Eco24I	GRGCYC	1 cut(s)	2068
Eco32I	GATATC	1 cut(s)	1128
Eco47I	GGWCC	4 cut(s)	1523, 1546, 1718, 1873
Eco57I	CTGAAG	4 cut(s)	826, 834, 1881, 1956
EcoO109I	RGGNCCY	2 cut(s)	1523, 1546
EcoRI	GAATTC	1 cut(s)	2194
EcoRII	CCWGG	3 cut(s)	1407, 1549, 2121
EcoRV	GATATC	1 cut(s)	1128
EcoT14I	CCWWGG	3 cut(s)	456, 704, 1945
EcoT38I	GRGCYC	1 cut(s)	2068
ErhI	CCWWGG	3 cut(s)	456, 704, 1945
Esp3I	CGTCTC	1 cut(s)	1114
FaeI	CATG	9 cut(s)	223, 460, 891, 910, 1269, 1315, 1401, 2147, 2314
FaqI	GGGAC	4 cut(s)	924, 1532, 1574, 1799
FatI	CATG	9 cut(s)	219, 456, 887, 906, 1265, 1311, 1397, 2143, 2310
FauI	CCCGC	3 cut(s)	2055, 2111, 2272
FauNDI	CATATG	1 cut(s)	2158
FbaI	TGATCA	2 cut(s)	1170, 2298
FblI	GTMKAC	2 cut(s)	427, 1580
Fnu4HI	GCNGC	7 cut(s)	302, 669, 918, 1017, 1957, 2006, 2257
FokI	GGATG	7 cut(s)	596, 1130, 1522, 1747, 1814, 2096, 2267
FriOI	GRGCYC	1 cut(s)	2068
Fsp4HI	GCNGC	7 cut(s)	302, 669, 918, 1017, 1957, 2006, 2257
FspBI	CTAG	2 cut(s)	1280, 1527
GlaI	GCGC	3 cut(s)	229, 270, 2218
GluI	GCNGC	7 cut(s)	302, 669, 918, 1017, 1957, 2006, 2257
GsaI	CCCAGC	2 cut(s)	1523, 1595
GsuI	CTGGAG	2 cut(s)	138, 2105
HaeII	RGCGCY	1 cut(s)	231
HaeIII	GGCC	8 cut(s)	373, 623, 837, 991, 1560, 1710, 1944, 2021
HapII	CCGG	4 cut(s)	351, 1584, 1625, 2148
HgaI	GACGC	2 cut(s)	1087, 1584
HhaI	GCGC	3 cut(s)	230, 271, 2219
Hin1I	GRCGYC	1 cut(s)	1770
Hin1II	CATG	9 cut(s)	223, 460, 891, 910, 1269, 1315, 1401, 2147, 2314
Hin6I	GCGC	3 cut(s)	228, 269, 2217
HinP1I	GCGC	3 cut(s)	228, 269, 2217
HincII	GTYRAC	2 cut(s)	7, 1034
HindII	GTYRAC	2 cut(s)	7, 1034
HinfI	GANTC	6 cut(s)	181, 678, 700, 1383, 1587, 2099
HpaI	GTTAAC	1 cut(s)	7
HpaII	CCGG	4 cut(s)	351, 1584, 1625, 2148
HphI	GGTGA	2 cut(s)	472, 1819
Hpy166II	GTNNAC	6 cut(s)	7, 428, 1034, 1334, 1581, 1873
Hpy188I	TCNGA	6 cut(s)	322, 845, 1137, 1305, 1755, 2277
Hpy188III	TCNNGA	5 cut(s)	117, 400, 930, 1202, 1851
Hpy8I	GTNNAC	6 cut(s)	7, 428, 1034, 1334, 1581, 1873
Hpy99I	CGWCG	2 cut(s)	146, 236
HpyAV	CCTTC	3 cut(s)	634, 1838, 1856
HpyCH4III	ACNGT	6 cut(s)	605, 660, 1666, 1742, 1870, 1918
HpyCH4IV	ACGT	1 cut(s)	1770
HpyF10VI	GCNNNNNNNGC	8 cut(s)	266, 824, 1013, 1025, 2014, 2144, 2153, 2253
HpyF3I	CTNAG	2 cut(s)	1057, 1149
HpySE526I	ACGT	1 cut(s)	1770
Hsp92I	GRCGYC	1 cut(s)	1770
Hsp92II	CATG	9 cut(s)	223, 460, 891, 910, 1269, 1315, 1401, 2147, 2314
HspAI	GCGC	3 cut(s)	228, 269, 2217
KflI	GGGWCCC	1 cut(s)	1546
Ksp22I	TGATCA	2 cut(s)	1170, 2298
KspAI	GTTAAC	1 cut(s)	7
Kzo9I	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
LmnI	GCTCC	3 cut(s)	276, 449, 2124
Lsp1109I	GCAGC	6 cut(s)	288, 655, 904, 1003, 1968, 1992
LweI	GCATC	7 cut(s)	136, 326, 512, 618, 1309, 1500, 2074
MaeI	CTAG	2 cut(s)	1280, 1527
MaeII	ACGT	1 cut(s)	1770
MaeIII	GTNAC	4 cut(s)	802, 1186, 1249, 2269
MalI	GATC	6 cut(s)	332, 647, 831, 1172, 1290, 2300
MbiI	CCGCTC	1 cut(s)	2259
MboI	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
MboII	GAAGA	7 cut(s)	24, 1038, 1417, 1477, 1639, 1906, 1962
MfeI	CAATTG	1 cut(s)	1191
MflI	RGATCY	2 cut(s)	330, 829
MhlI	GDGCHC	1 cut(s)	2068
MlsI	TGGCCA	2 cut(s)	837, 991
MluNI	TGGCCA	2 cut(s)	837, 991
MlyI	GAGTC	3 cut(s)	175, 1596, 2093
MmeI	TCCRAC	2 cut(s)	655, 1696
Mox20I	TGGCCA	2 cut(s)	837, 991
MscI	TGGCCA	2 cut(s)	837, 991
MseI	TTAA	8 cut(s)	6, 662, 750, 1613, 1659, 1776, 2180, 2207
MslI	CAYNNNNRTG	4 cut(s)	411, 546, 1372, 1832
Msp20I	TGGCCA	2 cut(s)	837, 991
MspA1I	CMGCKG	1 cut(s)	2267
MspI	CCGG	4 cut(s)	351, 1584, 1625, 2148
MspR9I	CCNGG	5 cut(s)	351, 1409, 1551, 2123, 2149
MunI	CAATTG	1 cut(s)	1191
Mva1269I	GAATGC	1 cut(s)	81
MvaI	CCWGG	3 cut(s)	1409, 1551, 2123
MvnI	CGCG	2 cut(s)	269, 1098
MwoI	GCNNNNNNNGC	8 cut(s)	266, 824, 1013, 1025, 2014, 2144, 2153, 2253
NciI	CCSGG	2 cut(s)	351, 2149
NcoI	CCATGG	1 cut(s)	456
NdeI	CATATG	1 cut(s)	2158
NdeII	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
NlaIII	CATG	9 cut(s)	223, 460, 891, 910, 1269, 1315, 1401, 2147, 2314
NlaIV	GGNNCC	5 cut(s)	1524, 1547, 1548, 1720, 1855
NmuCI	GTSAC	2 cut(s)	802, 1186
NspI	RCATGY	1 cut(s)	2147
PaeI	GCATGC	1 cut(s)	2147
PctI	GAATGC	1 cut(s)	81
PfeI	GAWTC	3 cut(s)	678, 700, 1383
PflMI	CCANNNNNTGG	1 cut(s)	619
PfoI	TCCNGGA	1 cut(s)	2121
PkrI	GCNGC	7 cut(s)	303, 670, 919, 1018, 1958, 2007, 2258
PleI	GAGTC	3 cut(s)	175, 1595, 2093
PpsI	GAGTC	3 cut(s)	175, 1595, 2093
PpuMI	RGGWCCY	2 cut(s)	1523, 1546
PshAI	GACNNNNGTC	1 cut(s)	240
PsiI	TTATAA	1 cut(s)	102
Psp5II	RGGWCCY	2 cut(s)	1523, 1546
Psp6I	CCWGG	3 cut(s)	1407, 1549, 2121
PspFI	CCCAGC	2 cut(s)	1519, 1591
PspGI	CCWGG	3 cut(s)	1407, 1549, 2121
PspN4I	GGNNCC	5 cut(s)	1524, 1547, 1548, 1720, 1855
PspPI	GGNCC	5 cut(s)	372, 1523, 1546, 1718, 1873
PspPPI	RGGWCCY	2 cut(s)	1523, 1546
PstI	CTGCAG	2 cut(s)	946, 2010
PsuI	RGATCY	2 cut(s)	330, 829
RsaI	GTAC	6 cut(s)	996, 1110, 1335, 1396, 1739, 1763
RsaNI	GTAC	6 cut(s)	995, 1109, 1334, 1395, 1738, 1762
RseI	CAYNNNNRTG	4 cut(s)	411, 546, 1372, 1832
SaqAI	TTAA	8 cut(s)	6, 662, 750, 1613, 1659, 1776, 2180, 2207
SatI	GCNGC	7 cut(s)	302, 669, 918, 1017, 1957, 2006, 2257
Sau3AI	GATC	6 cut(s)	330, 645, 829, 1170, 1288, 2298
Sau96I	GGNCC	5 cut(s)	372, 1523, 1546, 1718, 1873
ScaI	AGTACT	1 cut(s)	996
SchI	GAGTC	3 cut(s)	175, 1596, 2093
ScrFI	CCNGG	5 cut(s)	351, 1409, 1551, 2123, 2149
SduI	GDGCHC	1 cut(s)	2068
SfaNI	GCATC	7 cut(s)	136, 326, 512, 618, 1309, 1500, 2074
SfcI	CTRYAG	2 cut(s)	942, 2006
SinI	GGWCC	4 cut(s)	1523, 1546, 1718, 1873
SmiMI	CAYNNNNRTG	4 cut(s)	411, 546, 1372, 1832
SphI	GCATGC	1 cut(s)	2147
SsiI	CCGC	7 cut(s)	343, 827, 1876, 2062, 2104, 2257, 2265
SspMI	CTAG	2 cut(s)	1280, 1527
StyD4I	CCNGG	5 cut(s)	349, 1407, 1549, 2121, 2147
StyI	CCWWGG	3 cut(s)	456, 704, 1945
TaaI	ACNGT	6 cut(s)	605, 660, 1666, 1742, 1870, 1918
TaiI	ACGT	1 cut(s)	1773
TaqI	TCGA	4 cut(s)	399, 693, 1201, 1287
TatI	WGTACW	3 cut(s)	994, 1108, 1333
TauI	GCSGC	1 cut(s)	2259
TfiI	GAWTC	3 cut(s)	678, 700, 1383
Tru1I	TTAA	8 cut(s)	6, 662, 750, 1613, 1659, 1776, 2180, 2207
Tru9I	TTAA	8 cut(s)	6, 662, 750, 1613, 1659, 1776, 2180, 2207
TscAI	CASTG	4 cut(s)	174, 1576, 1873, 1993
TseFI	GTSAC	2 cut(s)	802, 1186
TseI	GCWGC	6 cut(s)	301, 668, 917, 1016, 1956, 2005
Tsp45I	GTSAC	2 cut(s)	802, 1186
TspDTI	ATGAA	7 cut(s)	616, 1300, 1356, 1517, 1816, 2187, 2327
TspGWI	ACGGA	2 cut(s)	717, 1138
TspRI	CASTG	4 cut(s)	174, 1576, 1873, 1993
Van91I	CCANNNNNTGG	1 cut(s)	619
VpaK11BI	GGWCC	4 cut(s)	1523, 1546, 1718, 1873
XapI	RAATTY	3 cut(s)	290, 535, 2194
XceI	RCATGY	1 cut(s)	2147
XmiI	GTMKAC	2 cut(s)	427, 1580
XspI	CTAG	2 cut(s)	1280, 1527
ZraI	GACGTC	1 cut(s)	1771
ZrmI	AGTACT	1 cut(s)	996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.