MD06G1102200.v1.1

Intracellular protein transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010735
Physical Location & Seq
Forward (+)
23904541 .. 23909676
5136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1102200.v1.1.491

Sequence Viewer

Length: 2493 bp
ATGATAAAACATCGCTGGAGAGAGCTAGCTAAGTCCTTTAATAATCATGTTGATCCTGAAACAACTGAGCGGCTGAAGAGGACTAAAACAGAAATTGAAAACAATGTGACAAGGATCTTCAAGCTTATAAAAATCGAAGACCAAGGGAAGAAGGATGTAAACCGAAAAGATTTGAAGCAAGAGTCGGAACTTGTTGCACTCATTGAGAACTTCTACCAACAGTACCAGTCACTATATGCTTTATATGATCATCTAGTAGGAGAGTCTGGGAGAATCGTTCATGGCAAGAAAGAACAAAAAGGTTTTTCCATGTCTCCCCCAAGCTCAGATTCCGAATATTATTCCTCAGAAGACGTTGAAGGTAACAATGCCAGATTGGAAAGAGCTGACAGCATCAAGCATGAACTTAAGAACGAATATTCCGAAGGATCTGGTGATCTGAAGCGAAAATCGGTCTCTGAAAATGGAGAAAAGGAGGCGATAAATTCAGAATATTTGGCAGCATTGAGAAAAATAAAACAATCAGAAATTGTAGACAATGATCTGAAGGATGAAAAAGTAAGAGAACTCTCAGCTCTTGTCGAAGCGCATGAGGCTCATGGGAATCATTCCTCAGCTCGGGTAAAAGAGTTAGAGGGACAGTTAACAGGCTTTAAAATGGAGTTGGAATCCTTATGCAGCCAGAAAAGAGATTTGGAAGCATGGAAAGAAGGTAAATCTGCTGAAGCTAAACAGCTAGGAGATAAAAATATCGGACTACATGCGCGAATTTTGGAACTTGAGTTAGTTTTAAAAGAAAGAGAGGATGAATTATCTGATTTCAAGAACAAACTCAAGGAAAATGAGGAGAGCTCAGCGTCGAAAATTTCAGACTTGATGACACATGCCGAAAATATGCAACAGGAAGTTGATTCGTTGTGTGCGCAAAAAGGTGAAATGGAGAAACAGATGGTGAGTAAGAAAAATGAATCAGCGACGCAAGTCAAAGGCTTAAGTGAGCAGGTCAATGCAATGCAGAAGGAATTGAAGATCCTGCACCAGCAGAAAATTGAATCACAAGCACAACTGGATGAGAAGAATAAAGAAATCTCCAATCATCTGCAACAGATGGAAATTCTGAGAGAGGAATTAACAAAAAAGGACACGGTTGAGAGGAAAATGATGGAAGAAAAAGGAAGTTTTCTTGTGAAATTGAATGACTTGGAATCGGAAGTGAACTCTCTACGCAACAAAAAAAGGAATGTGGAAAAGCAGATTAAAAACAGAAACCATGAGAACAGCAAGTTGAGACAGGAAAATGAAAGTCTTCTTTCTAGAATTTTCAGGTTGGAGAGAACTTTGACTGAGAGAGGGGATGAGATTTACGCTCTTCGTGGAGAATGCGAGCATGGAAAGAACGAAGCTTCTGCTCGACTTACTGAATTCACGACGCAGGTCAGCAATTTGAAACAGGAAATGGATTCCTTGCAGGCTCAGAAAAGCCAGTTGGACTTGCAGATTGAGATACAGAACAAGCGCTATTTGGAAAAACTGACTGAGATGGAAAATCTAAACGATAACTTGACAGACAAGATTGGCCAAATTGACAGAGATAACCAGAAGTATTTGGAAATACTGAGTGAGAAGGAAAATCAAAACCATAATTTGACAGTCAAGATTTCCGATCAGCAGAAAATTATCAAGAAACATGAAGAAACTTTCAAGAAGTTCAACAAGGAGCATAAACAAGCTAAAATTTGGTTTCCTCAATCTAAGTTAAATGTACAATTTGCCGAAAGGAAGATGGAAGAATTAGCAGAGAAGTACCGAATCAATCTTGAAGACAAAGTGCGCCTCTTGTACCAGAGGATCAGGGTAGCGGAACAAATACATAACGAAAACAAGGAGAGCTACAAGAAGTTCAAGGAGATGTACGAGAAAGAAAATGAAGATCTTAAAGAAAAGCTAGCAACGTATCAAGATCCAGACAGAAAGATGAAGCAAATATCGGAAACAGCGAAAAGTGCATTGCAAGGATTGGACTTAGTGGTGCTTAAATTTGAGGAGGGACACAAGAATTTTCAAAACCAGATTGCCAAAATATCGGAGGAGCTTGAGTCTGCAAAAACTTGGGTTAAAGAGAATGCTGGCAAGATCAAACGGTTAAAGCACAACGTAGATTACTTGACCACACAACTGAATGAGAAGGAAGAGCAAGAACGTGTGTTGAGAGAGAAAGTTTGGGAGTTGGAATCATCGGGGAGCAAGGAAACTGGAGAGAAGCTCAACTTAATGAAAGAGTTTAGCCAACTTGAGAGTCAGGTGGGAAATCTTGAGAGGGAAGTAAAGTACAAGGATGAAGATTTGTTGAGCCTTGGGGAGGAGAAGAGGGAGGCCATACGGCAACTCTGCGTGTTGGTTGATCATCACCGAAGCCGCTACTATGATCTGAAGGAAGCAGTGTCAAAGAGGTCAGCTGCAACAACTACTAGAGGCAGCAAGACTACAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
KIP1

Protein Analysis

831

Amino Acids

97.05

Weight (kDa)

6.29

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 13 - 83 1.9e-12 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
Acc16I TGCGCA 1 cut(s) 924
Acc36I ACCTGC 2 cut(s) 991, 1423
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 534
AccII CGCG 1 cut(s) 766
AciI CCGC 3 cut(s) 70, 1859, 2416
AclWI GGATC 6 cut(s) 47, 122, 436, 1024, 1856, 1955
AcoI YGGCCR 1 cut(s) 1576
AcsI RAATTY 9 cut(s) 484, 768, 864, 1113, 1317, 1421, 1734, 2036, 2056
AcuI CTGAAG 5 cut(s) 95, 461, 566, 744, 2450
AfaI GTAC 6 cut(s) 224, 1764, 1805, 1841, 1913, 2330
AfeI AGCGCT 1 cut(s) 1517
AfiI CCNNNNNNNGG 2 cut(s) 618, 2359
AflII CTTAAG 2 cut(s) 407, 991
AflIII ACRYGT 1 cut(s) 2200
AjuI GAANNNNNNNTTGG 6 cut(s) 313, 345, 677, 709, 1469, 1501
AloI GAACNNNNNNTCC 2 cut(s) 404, 436
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 3 cut(s) 318, 460, 1282
AlwI GGATC 6 cut(s) 47, 122, 436, 1024, 1856, 1955
Ama87I CYCGRG 1 cut(s) 618
Aor51HI AGCGCT 1 cut(s) 1517
AoxI GGCC 2 cut(s) 1576, 2373
ApeKI GCWGC 4 cut(s) 500, 678, 2456, 2475
ApoI RAATTY 9 cut(s) 484, 768, 864, 1113, 1317, 1421, 1734, 2036, 2056
Asp700I GAANNNNTTC 1 cut(s) 1305
AspLEI GCGC 5 cut(s) 589, 766, 925, 1518, 1833
AsuHPI GGTGA 4 cut(s) 446, 944, 964, 2399
AsuNHI GCTAGC 2 cut(s) 25, 1945
AvaI CYCGRG 1 cut(s) 618
BalI TGGCCA 1 cut(s) 1578
BanII GRGCYC 1 cut(s) 854
BbsI GAAGAC 4 cut(s) 144, 357, 1298, 1827
Bbv12I GWGCWC 1 cut(s) 854
BbvCI CCTCAGC 1 cut(s) 613
BbvI GCAGC 4 cut(s) 512, 690, 2443, 2487
BccI CCATC 5 cut(s) 943, 1102, 1156, 1534, 1777
BceAI ACGGC 1 cut(s) 2396
BcgI CGANNNNNNTGC 2 cut(s) 1388, 1422
BclI TGATCA 2 cut(s) 247, 2401
BcoDI GTCTC 3 cut(s) 318, 460, 1282
BfaI CTAG 6 cut(s) 26, 254, 737, 1314, 1946, 2469
BfoI RGCGCY 1 cut(s) 1519
BfrI CTTAAG 2 cut(s) 407, 991
BfuAI ACCTGC 2 cut(s) 991, 1423
BglII AGATCT 1 cut(s) 1930
BisI GCNGC 6 cut(s) 71, 501, 679, 2416, 2457, 2476
BlpI GCTNAGC 1 cut(s) 853
BlsI GCNGC 6 cut(s) 72, 502, 680, 2417, 2458, 2477
BmeT110I CYCGRG 1 cut(s) 618
BmsI GCATC 1 cut(s) 402
BmtI GCTAGC 2 cut(s) 29, 1949
BoxI GACNNNNGTC 2 cut(s) 980, 1433
BpiI GAAGAC 4 cut(s) 144, 357, 1298, 1827
BplI GAGNNNNNCTC 4 cut(s) 836, 868, 2247, 2279
BpmI CTGGAG 2 cut(s) 37, 2274
Bpu10I CCTNAGC 1 cut(s) 613
Bpu1102I GCTNAGC 1 cut(s) 853
BpuEI CTTGAG 5 cut(s) 800, 818, 2114, 2312, 2333
BsaI GGTCTC 1 cut(s) 460
BsaJI CCNNGG 2 cut(s) 142, 2353
BsaXI ACNNNNNCTCC 2 cut(s) 2081, 2111
Bsc4I CCNNNNNNNGG 2 cut(s) 618, 2359
Bse1I ACTGG 4 cut(s) 226, 1071, 1483, 2257
Bse3DI GCAATG 2 cut(s) 1017, 2006
BseDI CCNNGG 2 cut(s) 142, 2353
BseGI GGATG 6 cut(s) 160, 556, 811, 1075, 1360, 2341
BseLI CCNNNNNNNGG 2 cut(s) 618, 2359
BseMI GCAATG 2 cut(s) 1017, 2006
BseNI ACTGG 4 cut(s) 226, 1071, 1483, 2257
BseRI GAGGAG 4 cut(s) 860, 2057, 2102, 2375
BseXI GCAGC 4 cut(s) 512, 690, 2443, 2487
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 2 cut(s) 1578, 2375
BsiHKAI GWGCWC 1 cut(s) 854
BsiHKCI CYCGRG 1 cut(s) 618
BslFI GGGAC 2 cut(s) 651, 2061
BslI CCNNNNNNNGG 2 cut(s) 618, 2359
BsmAI GTCTC 3 cut(s) 318, 460, 1282
BsmFI GGGAC 2 cut(s) 651, 2061
BsmI GAATGC 2 cut(s) 1385, 2128
BsnI GGCC 2 cut(s) 1578, 2375
Bso31I GGTCTC 1 cut(s) 460
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 1 cut(s) 854
Bsp1407I TGTACA 1 cut(s) 1762
Bsp1720I GCTNAGC 1 cut(s) 853
BspACI CCGC 3 cut(s) 70, 1859, 2416
BspANI GGCC 2 cut(s) 1578, 2375
BspFNI CGCG 1 cut(s) 766
BspMI ACCTGC 2 cut(s) 991, 1423
BspOI GCTAGC 2 cut(s) 29, 1949
BspPI GGATC 6 cut(s) 47, 122, 436, 1024, 1856, 1955
BspQI GCTCTTC 2 cut(s) 1374, 2184
BspTI CTTAAG 2 cut(s) 407, 991
BspTNI GGTCTC 1 cut(s) 460
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 1017, 2006
BsrGI TGTACA 1 cut(s) 1762
BsrI ACTGG 4 cut(s) 226, 1071, 1483, 2257
BssECI CCNNGG 2 cut(s) 142, 2353
BssT1I CCWWGG 2 cut(s) 142, 2353
Bst4CI ACNGT 5 cut(s) 222, 642, 1147, 1651, 2142
Bst6I CTCTTC 4 cut(s) 71, 1374, 2184, 2360
BstAFI CTTAAG 2 cut(s) 407, 991
BstAUI TGTACA 1 cut(s) 1762
BstC8I GCNNGC 5 cut(s) 27, 1385, 1470, 1947, 2128
BstENI CCTNNNNNAGG 1 cut(s) 2357
BstF5I GGATG 6 cut(s) 160, 556, 811, 1075, 1360, 2341
BstFNI CGCG 1 cut(s) 766
BstH2I RGCGCY 1 cut(s) 1519
BstHHI GCGC 5 cut(s) 589, 766, 925, 1518, 1833
BstMAI GTCTC 3 cut(s) 318, 460, 1282
BstMWI GCNNNNNNNGC 2 cut(s) 593, 2003
BstNSI RCATGY 2 cut(s) 764, 887
BstPAI GACNNNNGTC 2 cut(s) 980, 1433
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 4 cut(s) 512, 690, 2443, 2487
BstV2I GAAGAC 4 cut(s) 144, 357, 1298, 1827
BstX2I RGATCY 5 cut(s) 114, 428, 1029, 1930, 1960
BstYI RGATCY 5 cut(s) 114, 428, 1029, 1930, 1960
BsuRI GGCC 2 cut(s) 1578, 2375
BtsCI GGATG 6 cut(s) 160, 556, 811, 1075, 1360, 2341
BtsI GCAGTG 1 cut(s) 2445
BtsIMutI CAGTG 1 cut(s) 2445
BveI ACCTGC 2 cut(s) 991, 1423
Cac8I GCNNGC 5 cut(s) 27, 1385, 1470, 1947, 2128
CfoI GCGC 5 cut(s) 589, 766, 925, 1518, 1833
CseI GACGC 3 cut(s) 846, 985, 1438
Csp6I GTAC 6 cut(s) 223, 1763, 1804, 1840, 1912, 2329
CviQI GTAC 6 cut(s) 223, 1763, 1804, 1840, 1912, 2329
DraI TTTAAA 2 cut(s) 655, 792
EaeI YGGCCR 1 cut(s) 1576
Eam1104I CTCTTC 4 cut(s) 71, 1374, 2184, 2360
EarI CTCTTC 4 cut(s) 71, 1374, 2184, 2360
Ecl136II GAGCTC 1 cut(s) 852
Eco130I CCWWGG 2 cut(s) 142, 2353
Eco24I GRGCYC 1 cut(s) 854
Eco31I GGTCTC 1 cut(s) 460
Eco47III AGCGCT 1 cut(s) 1517
Eco53kI GAGCTC 1 cut(s) 852
Eco57I CTGAAG 5 cut(s) 95, 461, 566, 744, 2450
Eco88I CYCGRG 1 cut(s) 618
EcoICRI GAGCTC 1 cut(s) 852
EcoNI CCTNNNNNAGG 1 cut(s) 2357
EcoRI GAATTC 1 cut(s) 1421
EcoT14I CCWWGG 2 cut(s) 142, 2353
EcoT38I GRGCYC 1 cut(s) 854
ErhI CCWWGG 2 cut(s) 142, 2353
FalI AAGNNNNNCTT 2 cut(s) 2252, 2284
FaqI GGGAC 2 cut(s) 651, 2061
FbaI TGATCA 2 cut(s) 247, 2401
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 6 cut(s) 71, 501, 679, 2416, 2457, 2476
FokI GGATG 6 cut(s) 167, 563, 818, 1082, 1367, 2348
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 6 cut(s) 71, 501, 679, 2416, 2457, 2476
FspBI CTAG 6 cut(s) 26, 254, 737, 1314, 1946, 2469
FspI TGCGCA 1 cut(s) 924
GlaI GCGC 5 cut(s) 588, 765, 924, 1517, 1832
GluI GCNGC 6 cut(s) 71, 501, 679, 2416, 2457, 2476
GsuI CTGGAG 2 cut(s) 37, 2274
HaeII RGCGCY 1 cut(s) 1519
HaeIII GGCC 2 cut(s) 1578, 2375
HgaI GACGC 3 cut(s) 846, 985, 1438
HhaI GCGC 5 cut(s) 589, 766, 925, 1518, 1833
Hin6I GCGC 5 cut(s) 587, 764, 923, 1516, 1831
HinP1I GCGC 5 cut(s) 587, 764, 923, 1516, 1831
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HindIII AAGCTT 2 cut(s) 122, 1401
HpaI GTTAAC 1 cut(s) 645
HphI GGTGA 4 cut(s) 446, 944, 964, 2399
Hpy166II GTNNAC 4 cut(s) 160, 535, 645, 1216
Hpy8I GTNNAC 4 cut(s) 160, 535, 645, 1216
Hpy99I CGWCG 3 cut(s) 862, 979, 1432
HpyAV CCTTC 9 cut(s) 145, 353, 419, 541, 704, 1012, 1618, 2179, 2425
HpyCH4III ACNGT 5 cut(s) 222, 642, 1147, 1651, 2142
HpyCH4IV ACGT 4 cut(s) 354, 1952, 2154, 2200
HpyF10VI GCNNNNNNNGC 2 cut(s) 593, 2003
HpySE526I ACGT 4 cut(s) 354, 1952, 2154, 2200
HspAI GCGC 5 cut(s) 587, 764, 923, 1516, 1831
Ksp22I TGATCA 2 cut(s) 247, 2401
KspAI GTTAAC 1 cut(s) 645
LguI GCTCTTC 2 cut(s) 1374, 2184
LmnI GCTCC 3 cut(s) 1717, 2089, 2241
Lsp1109I GCAGC 4 cut(s) 512, 690, 2443, 2487
LweI GCATC 1 cut(s) 402
MaeI CTAG 6 cut(s) 26, 254, 737, 1314, 1946, 2469
MaeII ACGT 4 cut(s) 354, 1952, 2154, 2200
MaeIII GTNAC 3 cut(s) 106, 228, 362
MbiI CCGCTC 1 cut(s) 70
MflI RGATCY 5 cut(s) 114, 428, 1029, 1930, 1960
MhlI GDGCHC 1 cut(s) 854
MlsI TGGCCA 1 cut(s) 1578
MluNI TGGCCA 1 cut(s) 1578
MlyI GAGTC 4 cut(s) 191, 272, 2105, 2305
MmeI TCCRAC 5 cut(s) 165, 645, 1308, 1467, 2208
Mox20I TGGCCA 1 cut(s) 1578
MroXI GAANNNNTTC 1 cut(s) 1305
MscI TGGCCA 1 cut(s) 1578
Msp20I TGGCCA 1 cut(s) 1578
MspA1I CMGCKG 1 cut(s) 2456
MspCI CTTAAG 2 cut(s) 407, 991
Mva1269I GAATGC 2 cut(s) 1385, 2128
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 2 cut(s) 593, 2003
NheI GCTAGC 2 cut(s) 25, 1945
NmuCI GTSAC 2 cut(s) 106, 228
NsbI TGCGCA 1 cut(s) 924
NspI RCATGY 2 cut(s) 764, 887
PciSI GCTCTTC 2 cut(s) 1374, 2184
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 1994
PctI GAATGC 2 cut(s) 1385, 2128
PdmI GAANNNNTTC 1 cut(s) 1305
PkrI GCNGC 6 cut(s) 72, 502, 680, 2417, 2458, 2477
PleI GAGTC 4 cut(s) 190, 271, 2104, 2304
PpsI GAGTC 4 cut(s) 190, 271, 2104, 2304
PshAI GACNNNNGTC 2 cut(s) 980, 1433
PsiI TTATAA 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 854
PsuI RGATCY 5 cut(s) 114, 428, 1029, 1930, 1960
PvuII CAGCTG 1 cut(s) 2456
RsaI GTAC 6 cut(s) 224, 1764, 1805, 1841, 1913, 2330
RsaNI GTAC 6 cut(s) 223, 1763, 1804, 1840, 1912, 2329
SacI GAGCTC 1 cut(s) 854
SapI GCTCTTC 2 cut(s) 1374, 2184
SatI GCNGC 6 cut(s) 71, 501, 679, 2416, 2457, 2476
SchI GAGTC 4 cut(s) 191, 272, 2105, 2305
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 1 cut(s) 402
SmlI CTYRAG 7 cut(s) 407, 779, 833, 991, 2093, 2291, 2312
SmoI CTYRAG 7 cut(s) 407, 779, 833, 991, 2093, 2291, 2312
SsiI CCGC 3 cut(s) 70, 1859, 2416
SspI AATATT 3 cut(s) 338, 419, 494
SspMI CTAG 6 cut(s) 26, 254, 737, 1314, 1946, 2469
SstI GAGCTC 1 cut(s) 854
StyI CCWWGG 2 cut(s) 142, 2353
TaaI ACNGT 5 cut(s) 222, 642, 1147, 1651, 2142
TaiI ACGT 4 cut(s) 357, 1955, 2157, 2203
TaqI TCGA 4 cut(s) 135, 582, 860, 1411
TaqII GACCGA 1 cut(s) 442
TatI WGTACW 2 cut(s) 1762, 2328
TauI GCSGC 2 cut(s) 73, 2418
TscAI CASTG 1 cut(s) 2445
TseFI GTSAC 2 cut(s) 106, 228
TseI GCWGC 4 cut(s) 500, 678, 2456, 2475
Tsp45I GTSAC 2 cut(s) 106, 228
TspRI CASTG 1 cut(s) 2445
Vha464I CTTAAG 2 cut(s) 407, 991
XagI CCTNNNNNAGG 1 cut(s) 2357
XapI RAATTY 9 cut(s) 484, 768, 864, 1113, 1317, 1421, 1734, 2036, 2056
XbaI TCTAGA 1 cut(s) 1313
XceI RCATGY 2 cut(s) 764, 887
XmiI GTMKAC 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 1305
XspI CTAG 6 cut(s) 26, 254, 737, 1314, 1946, 2469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.