MD14G1121400.v1.1

Intracellular protein transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010735
Physical Location & Seq
Forward (+)
19609517 .. 19614183
4667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1121400.v1.1.491

Sequence Viewer

Length: 2499 bp
ATGATAAAGCATCGCTGGAGGGAGATAGCTAAGTCCTTTAATAATCATGTTGATCCTGAAAAAACTGAGCAGCTGAAGAGGACTAAAACAGAAATTGAAAATAATGTGACGAGGATCTTCAAGCTTATAAAAAACGAAGACCCTGGGAAGAAAGATGGAAACCGAAAAGATTTGAAGAAAGAGTCAGAACTTGTTGCGCTCATTGAGAACTTCTACGAACAGTACCAGTCACTATACGCTTTGTATGATCATCTTGTAGGAGAGTCTGGGAGAATCGTTCGTGGCAAGAAAGAGCGAAAAGGTTTTTCCATGTCTCCCTCCAGCTCAGATTCCGAATACCATTCTTCAGAAGACATTGAAGGTAATAATGCCAGATTGGAAAGATCTGACAGCATCAAGCATGAACTTAAAAATGAACATTCGGAAGGCTCTGATGATCTGAAGCGAAAGTCGGTATCTGGAAATGGAGAAAAGGAAGCAATAAATTCAGAATATTTGGCGGCATTGAGAAAACTAACACAAGCAGAGATTGTAGACACTGATTTGAAGGATGAAGTTGATGAAAAAGTAAAGGAACTTTCAGCTCTTGTCGAAGCTCACGAGGCTCATGGGAATCATTCGTCAGCTCGAGTAAAAGAGTTGGAGGGACAGTTAACTGGTTTTAAAATGGAGTTGGAATCCTTGTGCAGCCAGAAACGAGATTTGGAAGCATGGAAGGAAGGAAAATCTGCTGAAGCTAAACAGCTAGGAGATAAAAATATTGGATTACATGCGCACATTTTGGAACTTGAGTTAGTTTTAAAAGAAAGAGATGATGAAATATCTGATCTCAAGAACAAACTCAAGGGAAATGAGGAGAGCTCAGTATCAAAAATTTCAGAATTGATGACACAAGTTGAAAACATGCAACAGGAAGTTGATTCGTTGTGTTTGCAAAAAGGTGAACTGGAGAAAAAGATGGTGAGTAAAAAGAATGAATCAGTAGCGCAAGTCAAGGGCTTAAGCGAGCACGTCAATGCAATGCGGAAGGAATTGAAGATCATTCGCCAGCAAAAAACTGAATTAGAAACGCAACTGGATAAGAAAAACAAAGAAATCTCCAAGCATCTGCAACAAATGGAAAATCTAAAAGATGAGTTAACAAAAAGGGAAACAGTTGAGAAGAAAATGATGGAAGACAAAGAACATTTTCTCGCGAAGGTGAAGGACTTGGAATCAGAAGTGAACTCTCTACGCAATAAAAAGAAGAATTTGGAAAAGCAGATTAAAAACAGAAACCATGAGAACAACAAGTTGAGACAAGAAAATGAGACTCTCATTTCTAGAATTTTTGGGTTGGAGAGAGCGTTGACCGAGAGAGGGGATGAGATTTACGCTCTTCGTGAAGAATGCGAAAATGGAAATATTGAAGCTTCTGCTCAACTTATGAAATTCACGACGCAGGTAAGCAATTTGAAACAAGAAATGGATTCCTTGCAGGCTCAGAAAAGCCAGTTGGATTTGCAGATTGAGATACAAAACAAGCACTATTTGAACAAACTGACTCAAATGGAAAATCGAAACGATAACTTGACAGACACGTTTGGCCGGATTGAGAGAGAGAACCAAAAGTATATGGAAATACTGAGTGAGAAGGAAAATCAAAACCATAACTTGACAGTCAAGATTTCCGATCAGCAGAAGATTATTAAGGAACATGAAGAAACTATCAAGAAGTTCAACATGGAGCATAAACAAGCTAAAATTTGGTTTTCGGAATCCAAGTTGAATGTACGATTTGTCGAACGGAAGATGGAAGAATTGGCAGAGAAGTATCGAACCAATCTTGAAGACAGCGTGCGCCTCTTATACCAGAGAATCAGGGTAGCAGAACAAATACACATTGAAAACAAGGAAAGCTACAAGAAGTTCAAAGAGATGTACGAGAAAGAAAACGAAGATCTTAAAGAAAAGCTAGCAACGTATCAAGATCCGGACACAAAGTTGAAGCAAATATCGAAAATAGCAAAAAGTACATTGCATGAATTGGACTTAGTGATGCTTAAATTTGAGGAGGGACACAAGAACTTTGAAAACCGGATTGCGAAAATGACAGAGGAGCTTGAGTCTGCAAAAACTTGGGTTAGCGAGAATGCCGGTGAGATCAAACGTTTAAAGCACAATGTAGATTTCTTAACCAGACAAATGAATGAAAAGGAAGAGCAGGAGAGTGTGTTGAGAGAAAAAGTTTGGAAGTTGGAAGCATCCGTGGGCAAGGAAGCTGGGGAGAGGCTGAACTTAGTAAAAGGGTTAAGCCAACTAGAGAGTAAGGTGGCAAATCTTGAGAAAGAAGTAAAGGACAAGGATGAAGACTTGCTGAGTCTTGGGGAGGAGAAGAGGGAGGCCATACGGCAGCTCTGCATGTTGATCGATCATCACCGGAGCCGCTACGATGATCTGAAGGAAGCGGTGTCAAAGAGGTCAGCTGCAACAGCTGCCGGAGGCAGGATTGCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
KIP1

Protein Analysis

833

Amino Acids

96.98

Weight (kDa)

6.24

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 13 - 83 2.9e-14 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
Acc16I TGCGCA 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 1432
AccI GTMKAC 1 cut(s) 534
AccII CGCG 1 cut(s) 1196
AccIII TCCGGA 1 cut(s) 1972
AciI CCGC 4 cut(s) 500, 1024, 2425, 2447
AclI AACGTT 1 cut(s) 2149
AclWI GGATC 3 cut(s) 47, 122, 1964
AcoI YGGCCR 1 cut(s) 1585
AcsI RAATTY 7 cut(s) 484, 873, 1249, 1326, 1430, 1743, 2045
AcuI CTGAAG 5 cut(s) 95, 330, 461, 753, 2459
AfaI GTAC 4 cut(s) 224, 1773, 1922, 2014
AfiI CCNNNNNNNGG 2 cut(s) 1359, 2484
AflII CTTAAG 1 cut(s) 1000
AflIII ACRYGT 1 cut(s) 1578
AjiI CACGTC 1 cut(s) 1012
AjnI CCWGG 1 cut(s) 142
AjuI GAANNNNNNNTTGG 4 cut(s) 686, 718, 1478, 1510
Alw21I GWGCWC 2 cut(s) 863, 1011
Alw26I GTCTC 3 cut(s) 318, 1291, 1304
AlwI GGATC 3 cut(s) 47, 122, 1964
Ama87I CYCGRG 1 cut(s) 627
Aor13HI TCCGGA 1 cut(s) 1972
AoxI GGCC 2 cut(s) 1585, 2382
ApeKI GCWGC 5 cut(s) 70, 687, 2392, 2465, 2474
ApoI RAATTY 7 cut(s) 484, 873, 1249, 1326, 1430, 1743, 2045
Asp700I GAANNNNTTC 1 cut(s) 1188
AspLEI GCGC 4 cut(s) 199, 775, 988, 1842
AsuHPI GGTGA 5 cut(s) 953, 973, 1213, 2150, 2408
AsuNHI GCTAGC 1 cut(s) 1954
AvaI CYCGRG 1 cut(s) 627
BanII GRGCYC 1 cut(s) 863
BauI CACGAG 1 cut(s) 599
BbsI GAAGAC 5 cut(s) 144, 357, 1182, 1836, 2355
Bbv12I GWGCWC 2 cut(s) 863, 1011
BbvI GCAGC 5 cut(s) 82, 699, 2404, 2452, 2461
BccI CCATC 4 cut(s) 149, 952, 1165, 1786
BceAI ACGGC 1 cut(s) 2405
BcgI CGANNNNNNTGC 2 cut(s) 2389, 2423
BciT130I CCWGG 1 cut(s) 144
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 3 cut(s) 318, 1291, 1304
BfaI CTAG 4 cut(s) 746, 1323, 1955, 2300
BfrI CTTAAG 1 cut(s) 1000
BfuAI ACCTGC 1 cut(s) 1432
BglII AGATCT 2 cut(s) 383, 1939
BisI GCNGC 7 cut(s) 71, 501, 688, 2393, 2425, 2466, 2475
BlsI GCNGC 7 cut(s) 72, 502, 689, 2394, 2426, 2467, 2476
Bme1390I CCNGG 1 cut(s) 144
BmeT110I CYCGRG 1 cut(s) 627
BmgBI CACGTC 1 cut(s) 1012
BmiI GGNNCC 1 cut(s) 2423
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 5 cut(s) 19, 402, 1114, 2028, 2252
BmtI GCTAGC 1 cut(s) 1958
BpiI GAAGAC 5 cut(s) 144, 357, 1182, 1836, 2355
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpmI CTGGAG 3 cut(s) 37, 304, 968
BpuEI CTTGAG 5 cut(s) 809, 815, 827, 2123, 2342
Bsa29I ATCGAT 1 cut(s) 2409
BsaJI CCNNGG 3 cut(s) 142, 143, 2247
BsaWI WCCGGW 3 cut(s) 1972, 2076, 2418
BsaXI ACNNNNNCTCC 8 cut(s) 635, 665, 1331, 1361, 2090, 2120, 2198, 2228
Bsc4I CCNNNNNNNGG 2 cut(s) 1359, 2484
Bse118I RCCGGY 1 cut(s) 2135
Bse1I ACTGG 5 cut(s) 226, 661, 951, 1080, 1492
Bse3DI GCAATG 2 cut(s) 1026, 2015
BseAI TCCGGA 1 cut(s) 1972
BseBI CCWGG 1 cut(s) 144
BseCI ATCGAT 1 cut(s) 2409
BseDI CCNNGG 3 cut(s) 142, 143, 2247
BseGI GGATG 4 cut(s) 556, 1369, 2243, 2350
BseLI CCNNNNNNNGG 2 cut(s) 1359, 2484
BseMI GCAATG 2 cut(s) 1026, 2015
BseMII CTCAG 6 cut(s) 57, 339, 876, 1496, 1616, 2348
BseNI ACTGG 5 cut(s) 226, 661, 951, 1080, 1492
BseRI GAGGAG 4 cut(s) 869, 2066, 2111, 2384
BseXI GCAGC 5 cut(s) 82, 699, 2404, 2452, 2461
BseYI CCCAGC 1 cut(s) 2261
BsgI GTGCAG 1 cut(s) 706
Bsh1236I CGCG 1 cut(s) 1196
BshFI GGCC 2 cut(s) 1587, 2384
BshVI ATCGAT 1 cut(s) 2409
BsiHKAI GWGCWC 2 cut(s) 863, 1011
BsiHKCI CYCGRG 1 cut(s) 627
BsiSI CCGG 6 cut(s) 1588, 1973, 2077, 2136, 2419, 2478
BslFI GGGAC 2 cut(s) 660, 2070
BslI CCNNNNNNNGG 2 cut(s) 1359, 2484
BsmAI GTCTC 3 cut(s) 318, 1291, 1304
BsmFI GGGAC 2 cut(s) 660, 2070
BsmI GAATGC 2 cut(s) 1394, 2137
BsnI GGCC 2 cut(s) 1587, 2384
BsoBI CYCGRG 1 cut(s) 627
Bsp1286I GDGCHC 2 cut(s) 863, 1011
Bsp13I TCCGGA 1 cut(s) 1972
Bsp68I TCGCGA 1 cut(s) 1196
BspACI CCGC 4 cut(s) 500, 1024, 2425, 2447
BspANI GGCC 2 cut(s) 1587, 2384
BspCNI CTCAG 6 cut(s) 58, 338, 875, 1495, 1617, 2349
BspDI ATCGAT 1 cut(s) 2409
BspEI TCCGGA 1 cut(s) 1972
BspFNI CGCG 1 cut(s) 1196
BspLI GGNNCC 1 cut(s) 2423
BspMI ACCTGC 1 cut(s) 1432
BspOI GCTAGC 1 cut(s) 1958
BspPI GGATC 3 cut(s) 47, 122, 1964
BspQI GCTCTTC 2 cut(s) 1383, 2193
BspTI CTTAAG 1 cut(s) 1000
BsrDI GCAATG 2 cut(s) 1026, 2015
BsrFI RCCGGY 1 cut(s) 2135
BsrI ACTGG 5 cut(s) 226, 661, 951, 1080, 1492
BssAI RCCGGY 1 cut(s) 2135
BssECI CCNNGG 3 cut(s) 142, 143, 2247
BssSI CACGAG 1 cut(s) 599
Bst2BI CACGAG 1 cut(s) 599
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 4 cut(s) 222, 651, 1156, 1660
Bst6I CTCTTC 4 cut(s) 71, 1383, 2193, 2369
BstAFI CTTAAG 1 cut(s) 1000
BstAPI GCANNNNNTGC 1 cut(s) 2474
BstC8I GCNNGC 5 cut(s) 1007, 1049, 1479, 1838, 1956
BstDSI CCRYGG 1 cut(s) 2247
BstF5I GGATG 4 cut(s) 556, 1369, 2243, 2350
BstFNI CGCG 1 cut(s) 1196
BstHHI GCGC 4 cut(s) 199, 775, 988, 1842
BstMAI GTCTC 3 cut(s) 318, 1291, 1304
BstMWI GCNNNNNNNGC 3 cut(s) 602, 2471, 2474
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 3 cut(s) 773, 907, 2404
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 1196
BstV1I GCAGC 5 cut(s) 82, 699, 2404, 2452, 2461
BstV2I GAAGAC 5 cut(s) 144, 357, 1182, 1836, 2355
BstX2I RGATCY 4 cut(s) 114, 383, 1939, 1969
BstYI RGATCY 4 cut(s) 114, 383, 1939, 1969
Bsu15I ATCGAT 1 cut(s) 2409
BsuRI GGCC 2 cut(s) 1587, 2384
BsuTUI ATCGAT 1 cut(s) 2409
BtgI CCRYGG 1 cut(s) 2247
BtrI CACGTC 1 cut(s) 1012
BtsCI GGATG 4 cut(s) 556, 1369, 2243, 2350
BtsIMutI CAGTG 1 cut(s) 537
BtuMI TCGCGA 1 cut(s) 1196
BveI ACCTGC 1 cut(s) 1432
Cac8I GCNNGC 5 cut(s) 1007, 1049, 1479, 1838, 1956
CfoI GCGC 4 cut(s) 199, 775, 988, 1842
Cfr10I RCCGGY 1 cut(s) 2135
ClaI ATCGAT 1 cut(s) 2409
CseI GACGC 1 cut(s) 1447
Csp6I GTAC 4 cut(s) 223, 1772, 1921, 2013
CviQI GTAC 4 cut(s) 223, 1772, 1921, 2013
DraI TTTAAA 3 cut(s) 664, 801, 2154
EaeI YGGCCR 1 cut(s) 1585
Eam1104I CTCTTC 4 cut(s) 71, 1383, 2193, 2369
EarI CTCTTC 4 cut(s) 71, 1383, 2193, 2369
Ecl136II GAGCTC 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 863
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 5 cut(s) 95, 330, 461, 753, 2459
Eco88I CYCGRG 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 861
EcoRII CCWGG 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 863
FaqI GGGAC 2 cut(s) 660, 2070
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 7 cut(s) 71, 501, 688, 2393, 2425, 2466, 2475
FokI GGATG 4 cut(s) 563, 1376, 2230, 2357
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 7 cut(s) 71, 501, 688, 2393, 2425, 2466, 2475
FspAI RTGCGCAY 1 cut(s) 774
FspBI CTAG 4 cut(s) 746, 1323, 1955, 2300
FspI TGCGCA 1 cut(s) 774
GlaI GCGC 4 cut(s) 198, 774, 987, 1841
GluI GCNGC 7 cut(s) 71, 501, 688, 2393, 2425, 2466, 2475
GsaI CCCAGC 1 cut(s) 2265
GsuI CTGGAG 3 cut(s) 37, 304, 968
HaeIII GGCC 2 cut(s) 1587, 2384
HapII CCGG 6 cut(s) 1588, 1973, 2077, 2136, 2419, 2478
HgaI GACGC 1 cut(s) 1447
HhaI GCGC 4 cut(s) 199, 775, 988, 1842
Hin6I GCGC 4 cut(s) 197, 773, 986, 1840
HinP1I GCGC 4 cut(s) 197, 773, 986, 1840
HincII GTYRAC 3 cut(s) 654, 1140, 1350
HindII GTYRAC 3 cut(s) 654, 1140, 1350
HindIII AAGCTT 2 cut(s) 122, 1410
HpaI GTTAAC 2 cut(s) 654, 1140
HpaII CCGG 6 cut(s) 1588, 1973, 2077, 2136, 2419, 2478
HphI GGTGA 5 cut(s) 953, 973, 1213, 2150, 2408
Hpy166II GTNNAC 6 cut(s) 535, 654, 944, 1140, 1225, 1350
Hpy8I GTNNAC 6 cut(s) 535, 654, 944, 1140, 1225, 1350
Hpy99I CGWCG 1 cut(s) 1441
HpyCH4III ACNGT 4 cut(s) 222, 651, 1156, 1660
HpyCH4IV ACGT 4 cut(s) 1011, 1580, 1961, 2149
HpyF10VI GCNNNNNNNGC 3 cut(s) 602, 2471, 2474
HpySE526I ACGT 4 cut(s) 1011, 1580, 1961, 2149
HspAI GCGC 4 cut(s) 197, 773, 986, 1840
Kpn2I TCCGGA 1 cut(s) 1972
Ksp22I TGATCA 1 cut(s) 247
KspAI GTTAAC 2 cut(s) 654, 1140
LguI GCTCTTC 2 cut(s) 1383, 2193
LmnI GCTCC 3 cut(s) 1726, 2098, 2421
Lsp1109I GCAGC 5 cut(s) 82, 699, 2404, 2452, 2461
LweI GCATC 5 cut(s) 19, 402, 1114, 2028, 2252
MaeI CTAG 4 cut(s) 746, 1323, 1955, 2300
MaeII ACGT 4 cut(s) 1011, 1580, 1961, 2149
MaeIII GTNAC 2 cut(s) 106, 228
MflI RGATCY 4 cut(s) 114, 383, 1939, 1969
MhlI GDGCHC 2 cut(s) 863, 1011
MlyI GAGTC 6 cut(s) 191, 272, 1306, 1537, 2114, 2368
MmeI TCCRAC 5 cut(s) 621, 654, 1317, 1476, 2217
MroI TCCGGA 1 cut(s) 1972
MroXI GAANNNNTTC 1 cut(s) 1188
MslI CAYNNNNRTG 1 cut(s) 1014
MspA1I CMGCKG 3 cut(s) 73, 2465, 2474
MspCI CTTAAG 1 cut(s) 1000
MspI CCGG 6 cut(s) 1588, 1973, 2077, 2136, 2419, 2478
MspR9I CCNGG 1 cut(s) 144
Mva1269I GAATGC 2 cut(s) 1394, 2137
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 1196
MwoI GCNNNNNNNGC 3 cut(s) 602, 2471, 2474
NheI GCTAGC 1 cut(s) 1954
NlaIV GGNNCC 1 cut(s) 2423
NmuCI GTSAC 2 cut(s) 106, 228
NruI TCGCGA 1 cut(s) 1196
NsbI TGCGCA 1 cut(s) 774
NspI RCATGY 3 cut(s) 773, 907, 2404
PaeR7I CTCGAG 1 cut(s) 627
PasI CCCWGGG 1 cut(s) 143
PciSI GCTCTTC 2 cut(s) 1383, 2193
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PctI GAATGC 2 cut(s) 1394, 2137
PdmI GAANNNNTTC 1 cut(s) 1188
PkrI GCNGC 7 cut(s) 72, 502, 689, 2394, 2426, 2467, 2476
PleI GAGTC 6 cut(s) 190, 271, 1306, 1537, 2113, 2367
PpsI GAGTC 6 cut(s) 190, 271, 1306, 1537, 2113, 2367
PsiI TTATAA 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 863
Psp1406I AACGTT 1 cut(s) 2149
Psp6I CCWGG 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 2261
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 2423
PspXI VCTCGAGB 1 cut(s) 627
PsuI RGATCY 4 cut(s) 114, 383, 1939, 1969
PvuII CAGCTG 3 cut(s) 73, 2465, 2474
RruI TCGCGA 1 cut(s) 1196
RsaI GTAC 4 cut(s) 224, 1773, 1922, 2014
RsaNI GTAC 4 cut(s) 223, 1772, 1921, 2013
RseI CAYNNNNRTG 1 cut(s) 1014
SacI GAGCTC 1 cut(s) 863
SapI GCTCTTC 2 cut(s) 1383, 2193
SatI GCNGC 7 cut(s) 71, 501, 688, 2393, 2425, 2466, 2475
SchI GAGTC 6 cut(s) 191, 272, 1306, 1537, 2114, 2368
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 2 cut(s) 863, 1011
SfaNI GCATC 5 cut(s) 19, 402, 1114, 2028, 2252
Sfr274I CTCGAG 1 cut(s) 627
SlaI CTCGAG 1 cut(s) 627
SmiMI CAYNNNNRTG 1 cut(s) 1014
SmlI CTYRAG 7 cut(s) 627, 788, 830, 842, 1000, 2102, 2321
SmoI CTYRAG 7 cut(s) 627, 788, 830, 842, 1000, 2102, 2321
SsiI CCGC 4 cut(s) 500, 1024, 2425, 2447
SspI AATATT 3 cut(s) 494, 760, 1405
SspMI CTAG 4 cut(s) 746, 1323, 1955, 2300
SstI GAGCTC 1 cut(s) 863
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 4 cut(s) 222, 651, 1156, 1660
TaiI ACGT 4 cut(s) 1014, 1583, 1964, 2152
TaqI TCGA 7 cut(s) 591, 628, 1558, 1782, 1816, 1997, 2409
TaqII GACCGA 1 cut(s) 1367
TatI WGTACW 1 cut(s) 2012
TauI GCSGC 2 cut(s) 503, 2427
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 2 cut(s) 106, 228
TseI GCWGC 5 cut(s) 70, 687, 2392, 2465, 2474
Tsp45I GTSAC 2 cut(s) 106, 228
TspGWI ACGGA 2 cut(s) 1801, 2236
TspRI CASTG 1 cut(s) 544
Vha464I CTTAAG 1 cut(s) 1000
XapI RAATTY 7 cut(s) 484, 873, 1249, 1326, 1430, 1743, 2045
XbaI TCTAGA 1 cut(s) 1322
XceI RCATGY 3 cut(s) 773, 907, 2404
XhoI CTCGAG 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 1188
XspI CTAG 4 cut(s) 746, 1323, 1955, 2300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.