MD06G1178600.v1.1

Nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002055
Physical Location & Seq
Forward (+)
31864355 .. 31869603
5249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1178600.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGGCAGGCGGAGGCGGCGGCGGAGCTGCAAAAGTGGATGAGCCACAACCACATCCACCAAAAGAACAGCTACCCAACATTTCTTACTGCATTACAAGTCCACCTCCATGGCCCGATGCCATTCTCCTTGGTTTTCAACACTACCTTGTGATGCTTGGAACTTCAGTTCTCATCCCCAGTGCTCTTGTTCCCCAGATGGGAGGAGGAAATGAAGAGAAGGCCAAGGTGATCCAGACCCTGCTTTTTGTGGCTGGTTTGAACACATTGCAACAGACTTTGTTTGGGACTAGACTACCCGCAGTGATTGGAGGGTCGTATACGTTTGTCCCGACCACAATTTCCATCATATTAGCTGGTCGATTCAGTGATAGTCAAGACCCTATAGAGAAATTTAAGAGGACAATGCGGGCAATCCAGGGTGCTCTTATTGTTGCTTCAACTCTTCAGATAGTCTTAGGCTTCAGTGGCCTTTGGCGTAATGTTGTAAGGTTTTTAAGCCCACTTTCAGTTGTTCCTTTGGTAGCAATGGTTGGTTTTGGACTCTACGAGTTAGGTTTTCCAGTGCTTGCCAAATGTGTGGAGATTGGACTGCCAGAGCTTGTCATTGTAGTATTTATTTCTCAGTATATGCCCCACATAATTCACAGAGGAAAGCCTATATTTGATCGTTTCGCTGTCATATTTTCCGTGGTGATTGTGTGGATATATGCTCACCTACTCACTGTTGGTGGAGCATATAATGGTACCGGCCCAAAAACACAGACAAGCTGCCGTACTGATCGTGCTGGACTTATAAATGCTGCTCCGTGGATTAGAGTTCCATATCCCTTCCAATGGGGAGCACCTTCCTTTGATGCTGGTGAAGCCTTTGCTATGATGGCAGCGGCTTTAGTTGCACTTGTTGAGTCCACTGGTGCTTTCATCGCTGTGTCAAGATATGCTAGTGCAACTCCAGTGCCACCTGCTATTCTGAGCCGTGGAGTTGGTTGGCAGGGAGTTGGCATTCTGGTTTCGGGCTTGTTTGGAACTGCGACTGGATCCTCAGTATCTGTTGAAAATGCTGGTCTTTTGGCGTTGACGCGTGTTGGCAGTCGAAGGGTTGTGCAAATATCTGCTGGTTTCATGATATTTTTCTCCATTCTGGGCAAATTTGGAGCACTCTTCGCGTCGATTCCAGGGCCAATCTTTGCTGCTTTGTACTGTCTGTTCTTTGCATATGTTGGTGCCGGAGGTCTTAGCTTTCTTCAGTTCTGCAACCTGAACAGCTTCCGAACAAAGTTCATATTGGGCTTCTCTATCTTCATTGGCTTGTCCGTGCCACAGTACTTCAATGAGTACACCGCCATCAACGGATACGGTCCAGTCCACACAGGAGGAAGATGGTTCAACGATATGATCAATGTCCCTCTACAATCAGAAGCTTTTGTCGCAGGCTGCGTCGCATATTTCTTGGACAACACGCTGCAACGAAAGGATAGTTCAGTTAAGAAAGACCGAGGTAAAACGTGGTGGAACAAGTTCCGGTCCTTCAAGGGGGATTCAAGGAGTGAGGAGTTTTATTCGCTACCATTCAATCTAAACAAGTACTTCCCATCCGTATGA

Protein Analysis

534

Amino Acids

57.56

Weight (kDa)

9.44

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 443 3.9e-67 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 1 cut(s) 970
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 2 cut(s) 743, 1223
AccI GTMKAC 1 cut(s) 317
AccII CGCG 2 cut(s) 1081, 1166
AciI CCGC 8 cut(s) 9, 15, 18, 21, 297, 406, 884, 1341
AclWI GGATC 3 cut(s) 223, 1032, 1045
AcsI RAATTY 2 cut(s) 389, 1148
AcuI CTGAAG 4 cut(s) 147, 428, 445, 1229
AfaI GTAC 6 cut(s) 745, 775, 1199, 1325, 1337, 1586
AfiI CCNNNNNNNGG 3 cut(s) 197, 834, 1533
AflIII ACRYGT 1 cut(s) 1079
AgsI TTSAA 9 cut(s) 137, 259, 438, 1055, 1330, 1387, 1531, 1542, 1573
AjnI CCWGG 2 cut(s) 414, 1174
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 8 cut(s) 26, 70, 353, 598, 768, 1239, 1266, 1421
AluI AGCT 8 cut(s) 26, 70, 353, 598, 768, 1239, 1266, 1421
Alw21I GWGCWC 4 cut(s) 184, 424, 844, 1159
AlwI GGATC 3 cut(s) 223, 1032, 1045
AlwNI CAGNNNCTG 2 cut(s) 238, 1049
AoxI GGCC 5 cut(s) 110, 219, 466, 748, 1178
ApeKI GCWGC 7 cut(s) 26, 768, 800, 881, 1190, 1434, 1462
ApoI RAATTY 2 cut(s) 389, 1148
Asp700I GAANNNNTTC 2 cut(s) 1265, 1517
Asp718I GGTACC 1 cut(s) 743
AspS9I GGNCC 5 cut(s) 111, 749, 1178, 1358, 1524
AsuHPI GGTGA 4 cut(s) 238, 703, 704, 872
AvaII GGWCC 2 cut(s) 1358, 1524
BamHI GGATCC 1 cut(s) 1037
BanI GGYRCC 2 cut(s) 743, 1223
Bbv12I GWGCWC 4 cut(s) 184, 424, 844, 1159
BbvI GCAGC 7 cut(s) 13, 755, 787, 893, 1177, 1421, 1449
BccI CCATC 6 cut(s) 190, 350, 871, 1352, 1374, 1600
BceAI ACGGC 2 cut(s) 756, 960
BciT130I CCWGG 2 cut(s) 416, 1176
BciVI GTATCC 1 cut(s) 1346
BclI TGATCA 1 cut(s) 1395
BfaI CTAG 2 cut(s) 288, 942
BfmI CTRYAG 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 970
BfuI GTATCC 1 cut(s) 1346
BmcAI AGTACT 2 cut(s) 1325, 1586
Bme1390I CCNGG 2 cut(s) 416, 1176
Bme18I GGWCC 2 cut(s) 1358, 1524
BmgT120I GGNCC 5 cut(s) 111, 749, 1178, 1358, 1524
BmiI GGNNCC 3 cut(s) 745, 1039, 1225
BmrFI CCNGG 2 cut(s) 416, 1176
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 3 cut(s) 106, 141, 844
BmuI ACTGGG 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 936
BsaJI CCNNGG 9 cut(s) 107, 127, 222, 415, 687, 806, 976, 1175, 1495
BsaWI WCCGGW 1 cut(s) 1521
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 834, 1533
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 6 cut(s) 177, 560, 916, 953, 1039, 1361
Bse3DI GCAATG 2 cut(s) 263, 531
BseBI CCWGG 2 cut(s) 416, 1176
BseDI CCNNGG 9 cut(s) 107, 127, 222, 415, 687, 806, 976, 1175, 1495
BseGI GGATG 4 cut(s) 43, 52, 171, 1592
BseLI CCNNNNNNNGG 3 cut(s) 197, 834, 1533
BseMI GCAATG 2 cut(s) 263, 531
BseMII CTCAG 3 cut(s) 635, 962, 1056
BseNI ACTGG 6 cut(s) 177, 560, 916, 953, 1039, 1361
BseRI GAGGAG 2 cut(s) 216, 1565
BseXI GCAGC 7 cut(s) 13, 755, 787, 893, 1177, 1421, 1449
Bsh1236I CGCG 2 cut(s) 1081, 1166
BshFI GGCC 5 cut(s) 112, 221, 468, 750, 1180
BshNI GGYRCC 2 cut(s) 743, 1223
BsiHKAI GWGCWC 4 cut(s) 184, 424, 844, 1159
BsiSI CCGG 3 cut(s) 747, 1227, 1522
BslFI GGGAC 3 cut(s) 298, 311, 1388
BslI CCNNNNNNNGG 3 cut(s) 197, 834, 1533
BsmFI GGGAC 3 cut(s) 298, 311, 1388
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 5 cut(s) 112, 221, 468, 750, 1180
Bsp1286I GDGCHC 4 cut(s) 184, 424, 844, 1159
Bsp143I GATC 5 cut(s) 228, 664, 778, 1037, 1395
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 8 cut(s) 9, 15, 18, 21, 297, 406, 884, 1341
BspANI GGCC 5 cut(s) 112, 221, 468, 750, 1180
BspCNI CTCAG 3 cut(s) 634, 963, 1055
BspFNI CGCG 2 cut(s) 1081, 1166
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 3 cut(s) 745, 1039, 1225
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 3 cut(s) 223, 1032, 1045
BspT107I GGYRCC 2 cut(s) 743, 1223
BsrDI GCAATG 2 cut(s) 263, 531
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 6 cut(s) 177, 560, 916, 953, 1039, 1361
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 9 cut(s) 107, 127, 222, 415, 687, 806, 976, 1175, 1495
BssMI GATC 5 cut(s) 228, 664, 778, 1037, 1395
BssNAI GTATAC 1 cut(s) 318
BssT1I CCWWGG 3 cut(s) 107, 127, 222
Bst1107I GTATAC 1 cut(s) 318
Bst2UI CCWGG 2 cut(s) 416, 1176
Bst4CI ACNGT 4 cut(s) 724, 1202, 1323, 1358
Bst6I CTCTTC 3 cut(s) 207, 447, 1166
BstC8I GCNNGC 4 cut(s) 7, 408, 567, 1432
BstDEI CTNAG 5 cut(s) 454, 621, 971, 1042, 1235
BstDSI CCRYGG 4 cut(s) 107, 687, 806, 976
BstF5I GGATG 4 cut(s) 43, 52, 171, 1592
BstFNI CGCG 2 cut(s) 1081, 1166
BstKTI GATC 5 cut(s) 231, 667, 781, 1040, 1398
BstMBI GATC 5 cut(s) 228, 664, 778, 1037, 1395
BstMWI GCNNNNNNNGC 8 cut(s) 15, 465, 863, 878, 893, 923, 1163, 1427
BstNI CCWGG 2 cut(s) 416, 1176
BstSCI CCNGG 2 cut(s) 414, 1174
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 2 cut(s) 1081, 1166
BstV1I GCAGC 7 cut(s) 13, 755, 787, 893, 1177, 1421, 1449
BstX2I RGATCY 1 cut(s) 1037
BstXI CCANNNNNNTGG 2 cut(s) 108, 577
BstYI RGATCY 1 cut(s) 1037
BstZ17I GTATAC 1 cut(s) 318
BsuI GTATCC 1 cut(s) 1346
BsuRI GGCC 5 cut(s) 112, 221, 468, 750, 1180
BtgI CCRYGG 4 cut(s) 107, 687, 806, 976
BtgZI GCGATG 1 cut(s) 907
BtsCI GGATG 4 cut(s) 43, 52, 171, 1592
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 8 cut(s) 184, 306, 370, 469, 567, 720, 909, 960
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 4 cut(s) 7, 408, 567, 1432
CaiI CAGNNNCTG 2 cut(s) 238, 1049
CciI TCATGA 1 cut(s) 1122
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 5 cut(s) 111, 749, 1178, 1358, 1524
CseI GACGC 3 cut(s) 1087, 1155, 1426
Csp6I GTAC 6 cut(s) 744, 774, 1198, 1324, 1336, 1585
CviAII CATG 2 cut(s) 108, 1123
CviQI GTAC 6 cut(s) 744, 774, 1198, 1324, 1336, 1585
DdeI CTNAG 5 cut(s) 454, 621, 971, 1042, 1235
DpnI GATC 5 cut(s) 230, 666, 780, 1039, 1397
DpnII GATC 5 cut(s) 228, 664, 778, 1037, 1395
Eam1104I CTCTTC 3 cut(s) 207, 447, 1166
EarI CTCTTC 3 cut(s) 207, 447, 1166
EciI GGCGGA 2 cut(s) 24, 36
Eco130I CCWWGG 3 cut(s) 107, 127, 222
Eco47I GGWCC 2 cut(s) 1358, 1524
Eco57I CTGAAG 4 cut(s) 147, 428, 445, 1229
EcoRII CCWGG 2 cut(s) 414, 1174
EcoT14I CCWWGG 3 cut(s) 107, 127, 222
ErhI CCWWGG 3 cut(s) 107, 127, 222
FaeI CATG 2 cut(s) 111, 1126
FaqI GGGAC 3 cut(s) 298, 311, 1388
FatI CATG 2 cut(s) 107, 1122
FauI CCCGC 2 cut(s) 304, 399
FauNDI CATATG 1 cut(s) 1216
FbaI TGATCA 1 cut(s) 1395
FblI GTMKAC 1 cut(s) 317
FokI GGATG 4 cut(s) 39, 50, 158, 1579
FspBI CTAG 2 cut(s) 288, 942
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 5 cut(s) 112, 221, 468, 750, 1180
HapII CCGG 3 cut(s) 747, 1227, 1522
HgaI GACGC 3 cut(s) 1087, 1155, 1426
Hin1II CATG 2 cut(s) 111, 1126
HincII GTYRAC 1 cut(s) 1077
HindII GTYRAC 1 cut(s) 1077
HindIII AAGCTT 1 cut(s) 1419
HinfI GANTC 5 cut(s) 360, 540, 905, 1171, 1538
HpaII CCGG 3 cut(s) 747, 1227, 1522
HphI GGTGA 4 cut(s) 238, 703, 704, 872
Hpy166II GTNNAC 6 cut(s) 101, 318, 909, 1077, 1338, 1366
Hpy188I TCNGA 4 cut(s) 447, 972, 1271, 1417
Hpy188III TCNNGA 5 cut(s) 232, 328, 374, 933, 1123
Hpy8I GTNNAC 6 cut(s) 101, 318, 909, 1077, 1338, 1366
Hpy99I CGWCG 2 cut(s) 1171, 1442
HpyAV CCTTC 5 cut(s) 211, 838, 855, 1089, 1537
HpyCH4III ACNGT 4 cut(s) 724, 1202, 1323, 1358
HpyCH4IV ACGT 2 cut(s) 320, 1505
HpyCH4V TGCA 9 cut(s) 29, 90, 268, 896, 947, 1105, 1214, 1254, 1465
HpyF10VI GCNNNNNNNGC 8 cut(s) 15, 465, 863, 878, 893, 923, 1163, 1427
HpyF3I CTNAG 5 cut(s) 454, 621, 971, 1042, 1235
HpySE526I ACGT 2 cut(s) 320, 1505
Hsp92II CATG 2 cut(s) 111, 1126
KpnI GGTACC 1 cut(s) 747
Ksp22I TGATCA 1 cut(s) 1395
Kzo9I GATC 5 cut(s) 228, 664, 778, 1037, 1395
LmnI GCTCC 5 cut(s) 23, 731, 808, 839, 1154
Lsp1109I GCAGC 7 cut(s) 13, 755, 787, 893, 1177, 1421, 1449
LweI GCATC 3 cut(s) 106, 141, 844
MaeI CTAG 2 cut(s) 288, 942
MaeII ACGT 2 cut(s) 320, 1505
MalI GATC 5 cut(s) 230, 666, 780, 1039, 1397
MboI GATC 5 cut(s) 228, 664, 778, 1037, 1395
MboII GAAGA 6 cut(s) 224, 434, 1153, 1235, 1291, 1389
MflI RGATCY 1 cut(s) 1037
MhlI GDGCHC 4 cut(s) 184, 424, 844, 1159
MluCI AATT 4 cut(s) 336, 389, 639, 1148
MluI ACGCGT 1 cut(s) 1079
MlyI GAGTC 2 cut(s) 534, 914
MroXI GAANNNNTTC 2 cut(s) 1265, 1517
MseI TTAA 3 cut(s) 393, 494, 1485
MslI CAYNNNNRTG 3 cut(s) 106, 926, 1597
MspA1I CMGCKG 1 cut(s) 884
MspI CCGG 3 cut(s) 747, 1227, 1522
MspR9I CCNGG 2 cut(s) 416, 1176
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 2 cut(s) 416, 1176
MvnI CGCG 2 cut(s) 1081, 1166
MwoI GCNNNNNNNGC 8 cut(s) 15, 465, 863, 878, 893, 923, 1163, 1427
NcoI CCATGG 1 cut(s) 107
NdeI CATATG 1 cut(s) 1216
NdeII GATC 5 cut(s) 228, 664, 778, 1037, 1395
NlaIII CATG 2 cut(s) 111, 1126
NlaIV GGNNCC 3 cut(s) 745, 1039, 1225
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 970
PcsI WCGNNNNNNNCGW 2 cut(s) 326, 1434
PctI GAATGC 1 cut(s) 1002
PdmI GAANNNNTTC 2 cut(s) 1265, 1517
PfeI GAWTC 3 cut(s) 360, 1171, 1538
PleI GAGTC 2 cut(s) 534, 913
PpsI GAGTC 2 cut(s) 534, 913
PsiI TTATAA 1 cut(s) 794
Psp6I CCWGG 2 cut(s) 414, 1174
PspGI CCWGG 2 cut(s) 414, 1174
PspN4I GGNNCC 3 cut(s) 745, 1039, 1225
PspPI GGNCC 5 cut(s) 111, 749, 1178, 1358, 1524
PsrI GAACNNNNNNTAC 2 cut(s) 1190, 1222
PstNI CAGNNNCTG 2 cut(s) 238, 1049
PsuI RGATCY 1 cut(s) 1037
RsaI GTAC 6 cut(s) 745, 775, 1199, 1325, 1337, 1586
RsaNI GTAC 6 cut(s) 744, 774, 1198, 1324, 1336, 1585
RseI CAYNNNNRTG 3 cut(s) 106, 926, 1597
SaqAI TTAA 3 cut(s) 393, 494, 1485
Sau3AI GATC 5 cut(s) 228, 664, 778, 1037, 1395
Sau96I GGNCC 5 cut(s) 111, 749, 1178, 1358, 1524
ScaI AGTACT 2 cut(s) 1325, 1586
SchI GAGTC 2 cut(s) 534, 914
ScrFI CCNGG 2 cut(s) 416, 1176
SduI GDGCHC 4 cut(s) 184, 424, 844, 1159
SfaNI GCATC 3 cut(s) 106, 141, 844
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 2 cut(s) 1358, 1524
SmiMI CAYNNNNRTG 3 cut(s) 106, 926, 1597
Sse9I AATT 4 cut(s) 336, 389, 639, 1148
SsiI CCGC 8 cut(s) 9, 15, 18, 21, 297, 406, 884, 1341
SspMI CTAG 2 cut(s) 288, 942
StyD4I CCNGG 2 cut(s) 414, 1174
StyI CCWWGG 3 cut(s) 107, 127, 222
TaaI ACNGT 4 cut(s) 724, 1202, 1323, 1358
TaiI ACGT 2 cut(s) 323, 1508
TaqI TCGA 3 cut(s) 358, 1093, 1169
TaqII GACCGA 1 cut(s) 1509
TasI AATT 4 cut(s) 336, 389, 639, 1148
TatI WGTACW 4 cut(s) 1197, 1323, 1335, 1584
TauI GCSGC 3 cut(s) 18, 21, 887
TfiI GAWTC 3 cut(s) 360, 1171, 1538
Tru1I TTAA 3 cut(s) 393, 494, 1485
Tru9I TTAA 3 cut(s) 393, 494, 1485
TscAI CASTG 8 cut(s) 184, 306, 370, 469, 567, 727, 916, 960
TseI GCWGC 7 cut(s) 26, 768, 800, 881, 1190, 1434, 1462
TspDTI ATGAA 5 cut(s) 225, 910, 1111, 1270, 1291
TspGWI ACGGA 5 cut(s) 676, 795, 1303, 1365, 1585
TspRI CASTG 8 cut(s) 184, 306, 370, 469, 567, 727, 916, 960
VpaK11BI GGWCC 2 cut(s) 1358, 1524
XapI RAATTY 2 cut(s) 389, 1148
XmiI GTMKAC 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 1265, 1517
XspI CTAG 2 cut(s) 288, 942
ZrmI AGTACT 2 cut(s) 1325, 1586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.