Rw7G002020

Nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002055
Physical Location & Seq
Forward (+)
1661466 .. 1666458
4993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002020.1

Sequence Viewer

Length: 1596 bp
ATGGCTGGAGGAGGTGCTGCAAAGGTCGATGAGCCACAGCCTCATCCACCCAAAGAACAACTACCTGGCATTTCTTACTGCATTACAAGTCCACCTCCATGGCCCGAGGCCATTCTCCTTGGGTTTCAACATTATCTTGTGATGCTTGGCACTTCAGTTCTCATTCCTAGTGCTCTTGTTCCCCAGATGGGAGGTGGAAATGAGGAGAAGGCCAAGGTGATCCAGACCCTACTCTTTGTTGCTGGTTTGAACACCTTGAAGCAAACATTGTTTGGGACTAGACTACCTGCAGTGATTGGAGGGTCATACACGTTTGTTCCCACCACAGTTTCAATCATATTAGCTAGTCGATTCAGTGATTCTCAAGACCTCCCTATAGACAAATTTAAGAGAACAATGCGGGCAATTCAAGGAGCTCTTATTGTGGCCTCAACCCTTCAGATAGTCTTAGGCTTCAGTGGCCTTTGGCGTAATGTTGCAAGGTTTTTAAGTCCACTGTCAATTGTTCCATTGATAGGACTAGTTGGTTTTGGACTCTACGAGTTAGGTTTTCCTCTGCTTGCCAAATGTGTTGAGATCGGACTGCCAGAGCTTATCATTTTAGTATTTGTCTCTCAGTATTTGCCCCACATATTTCACAGAGGAAAACATGTATTTGATCGTTTTGCTGTTCTATTTGCCATTGTGATCGTGTGGATTTATGCTCATCTGCTCACTGTTGGCGGGGCATATAATGATTCAGGCCCCAAAACACAAGCAAGTTGCCGTACTGACCGTGCTGGACTTATAGATGGTGCTCCATGGATAAGAGTTCCGTATCCCTTCCAATGGGGAGCACCTACATTTGATGCTGGTGAAGCCTTTGCTATGATGACTGCGTCTTTTGTTGCTCTCGTAGAGTCCACTGGTGCTTTCATTGTTGTATCAAGGTATGCTAGTGCAACTCCAGTGCCACCTGCTATTCTGAGTCGTGGGGTTGGTTGGCAGGGTGTTGGGATTCTGATTTCCGGGCTATTTGGAACTGTGACCGGATCCTCAGTATCTGTAGAGAATGCTGGTCTTTTGGCGTTAACACGTGTTGGCAGTCGAAGGGTTGTGCAAATATCTGCTGGGTTCATGATATTTTTCTCCATTCTGGGCAAATTTGGAGCAGTCTTCGCTTCAATCCCAGGGCCCATCATTGCTGCTTTATATTGTCTGTTCTTTGCTTATGTTGGTTCGGGGGGTCTTAGCTTTCTCCAGTTCTGCAACCTGAACAGCTTTCGAACCAAGTTCATATTAGGCTTCTCTATCTTCATGGGTTTGTCTATACCACAGTACTTCAATGAGTACACCCTCCTCAACGGTTATGGTCCAGTCCACACAGGAGGAAGATGGTTTAATGATATGATCAATGTCCCTTTCCAATCGGAAGCGTTTGTGGCGGGTATTATTGCATATTTCTTGGACAACACGCTGCATCGAAATGACAACACAGTTAGGAAGGACAGGGGTAAACATTGGTGGGACAAGTTCCGGTCATTCAAGGGTGATTCAAGGAGTGAGGAGTTCTATTCATTGCCGTTCAATCTAAACAAGTATTTTCCATCTGTATGA

Protein Analysis

531

Amino Acids

57.86

Weight (kDa)

9.3

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 37 - 441 8.5e-66 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc36I ACCTGC 2 cut(s) 295, 964
AciI CCGC 3 cut(s) 400, 723, 1424
AclWI GGATC 3 cut(s) 214, 1026, 1039
AcsI RAATTY 2 cut(s) 383, 1142
AcuI CTGAAG 3 cut(s) 138, 422, 439
AcvI CACGTG 1 cut(s) 1076
AfaI GTAC 3 cut(s) 769, 1319, 1331
AfiI CCNNNNNNNGG 3 cut(s) 188, 515, 828
AflIII ACRYGT 4 cut(s) 309, 649, 1073, 1075
AhlI ACTAGT 1 cut(s) 520
AjnI CCWGG 2 cut(s) 64, 1168
AluBI AGCT 5 cut(s) 344, 416, 592, 1233, 1260
AluI AGCT 5 cut(s) 344, 416, 592, 1233, 1260
Alw21I GWGCWC 4 cut(s) 175, 418, 799, 838
Alw26I GTCTC 1 cut(s) 616
AlwI GGATC 3 cut(s) 214, 1026, 1039
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 7 cut(s) 101, 108, 210, 426, 460, 742, 1172
ApaI GGGCCC 1 cut(s) 1176
ApeKI GCWGC 3 cut(s) 17, 1184, 1456
ApoI RAATTY 2 cut(s) 383, 1142
AspS9I GGNCC 5 cut(s) 102, 743, 1172, 1173, 1352
AsuC2I CCSGG 1 cut(s) 1009
AsuHPI GGTGA 3 cut(s) 229, 866, 1541
AsuII TTCGAA 1 cut(s) 1264
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 1352
BaeGI GKGCMC 1 cut(s) 1176
BamHI GGATCC 1 cut(s) 1031
BanII GRGCYC 2 cut(s) 418, 1176
BbrPI CACGTG 1 cut(s) 1076
BbsI GAAGAC 1 cut(s) 1147
Bbv12I GWGCWC 4 cut(s) 175, 418, 799, 838
BbvI GCAGC 3 cut(s) 4, 1171, 1443
BccI CCATC 5 cut(s) 181, 785, 1184, 1368, 1594
BceAI ACGGC 2 cut(s) 750, 1546
BciT130I CCWGG 2 cut(s) 66, 1170
BciVI GTATCC 1 cut(s) 828
BclI TGATCA 1 cut(s) 1389
BcnI CCSGG 1 cut(s) 1009
BcoDI GTCTC 1 cut(s) 616
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 5 cut(s) 168, 279, 345, 521, 936
BfmI CTRYAG 3 cut(s) 288, 375, 1044
BfuAI ACCTGC 2 cut(s) 295, 964
BfuI GTATCC 1 cut(s) 828
BisI GCNGC 3 cut(s) 18, 1185, 1457
BlsI GCNGC 3 cut(s) 19, 1186, 1458
BmcAI AGTACT 1 cut(s) 1319
Bme1390I CCNGG 3 cut(s) 66, 1009, 1170
Bme18I GGWCC 1 cut(s) 1352
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 5 cut(s) 102, 743, 1172, 1173, 1352
BmiI GGNNCC 3 cut(s) 745, 1033, 1174
BmrFI CCNGG 3 cut(s) 66, 1009, 1170
BmsI GCATC 3 cut(s) 132, 838, 1468
BpiI GAAGAC 1 cut(s) 1147
BpmI CTGGAG 3 cut(s) 27, 930, 1223
Bpu14I TTCGAA 1 cut(s) 1264
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 1009
BsaAI YACGTR 1 cut(s) 1076
BsaJI CCNNGG 7 cut(s) 98, 105, 118, 213, 800, 1168, 1169
BsaWI WCCGGW 2 cut(s) 1028, 1515
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 515, 828
Bse1I ACTGG 4 cut(s) 910, 947, 1240, 1355
Bse3DI GCAATG 2 cut(s) 1179, 1556
BseBI CCWGG 2 cut(s) 66, 1170
BseDI CCNNGG 7 cut(s) 98, 105, 118, 213, 800, 1168, 1169
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 3 cut(s) 188, 515, 828
BseMI GCAATG 2 cut(s) 1179, 1556
BseMII CTCAG 3 cut(s) 629, 956, 1050
BseNI ACTGG 4 cut(s) 910, 947, 1240, 1355
BseRI GAGGAG 4 cut(s) 24, 218, 1328, 1559
BseSI GKGCMC 1 cut(s) 1176
BseXI GCAGC 3 cut(s) 4, 1171, 1443
BseYI CCCAGC 1 cut(s) 1109
BshFI GGCC 7 cut(s) 103, 110, 212, 428, 462, 744, 1174
BsiHKAI GWGCWC 4 cut(s) 175, 418, 799, 838
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 3 cut(s) 1008, 1029, 1516
BslFI GGGAC 3 cut(s) 289, 1382, 1520
BslI CCNNNNNNNGG 3 cut(s) 188, 515, 828
BsmAI GTCTC 1 cut(s) 616
BsmFI GGGAC 3 cut(s) 289, 1382, 1520
BsmI GAATGC 1 cut(s) 1057
BsnI GGCC 7 cut(s) 103, 110, 212, 428, 462, 744, 1174
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 1264
Bsp120I GGGCCC 1 cut(s) 1172
Bsp1286I GDGCHC 5 cut(s) 175, 418, 799, 838, 1176
Bsp143I GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
Bsp19I CCATGG 2 cut(s) 98, 800
BspACI CCGC 3 cut(s) 400, 723, 1424
BspANI GGCC 7 cut(s) 103, 110, 212, 428, 462, 744, 1174
BspCNI CTCAG 3 cut(s) 628, 957, 1049
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 3 cut(s) 745, 1033, 1174
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 2 cut(s) 295, 964
BspPI GGATC 3 cut(s) 214, 1026, 1039
BspT104I TTCGAA 1 cut(s) 1264
BsrDI GCAATG 2 cut(s) 1179, 1556
BsrI ACTGG 4 cut(s) 910, 947, 1240, 1355
BssECI CCNNGG 7 cut(s) 98, 105, 118, 213, 800, 1168, 1169
BssMI GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
BssT1I CCWWGG 4 cut(s) 98, 118, 213, 800
Bst2UI CCWGG 2 cut(s) 66, 1170
Bst4CI ACNGT 8 cut(s) 328, 498, 718, 776, 1024, 1317, 1346, 1477
BstBAI YACGTR 1 cut(s) 1076
BstBI TTCGAA 1 cut(s) 1264
BstC8I GCNNGC 2 cut(s) 402, 561
BstDEI CTNAG 5 cut(s) 448, 615, 965, 1036, 1229
BstDSI CCRYGG 2 cut(s) 98, 800
BstF5I GGATG 1 cut(s) 43
BstKTI GATC 6 cut(s) 222, 579, 661, 690, 1034, 1392
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
BstMWI GCNNNNNNNGC 4 cut(s) 459, 857, 1157, 1421
BstNI CCWGG 2 cut(s) 66, 1170
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 3 cut(s) 64, 1007, 1168
BstSFI CTRYAG 3 cut(s) 288, 375, 1044
BstSLI GKGCMC 1 cut(s) 1176
BstV1I GCAGC 3 cut(s) 4, 1171, 1443
BstV2I GAAGAC 1 cut(s) 1147
BstX2I RGATCY 1 cut(s) 1031
BstXI CCANNNNNNTGG 1 cut(s) 99
BstYI RGATCY 1 cut(s) 1031
BsuI GTATCC 1 cut(s) 828
BsuRI GGCC 7 cut(s) 103, 110, 212, 428, 462, 744, 1174
BtgI CCRYGG 2 cut(s) 98, 800
BtsCI GGATG 1 cut(s) 43
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 7 cut(s) 297, 361, 463, 494, 714, 903, 954
BveI ACCTGC 2 cut(s) 295, 964
Cac8I GCNNGC 2 cut(s) 402, 561
CaiI CAGNNNCTG 1 cut(s) 1043
CciI TCATGA 1 cut(s) 1116
Cfr13I GGNCC 5 cut(s) 102, 743, 1172, 1173, 1352
CseI GACGC 1 cut(s) 867
Csp6I GTAC 3 cut(s) 768, 1318, 1330
CviAII CATG 5 cut(s) 99, 650, 801, 1117, 1297
CviQI GTAC 3 cut(s) 768, 1318, 1330
DdeI CTNAG 5 cut(s) 448, 615, 965, 1036, 1229
DpnI GATC 6 cut(s) 221, 578, 660, 689, 1033, 1391
DpnII GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 4 cut(s) 98, 118, 213, 800
Eco24I GRGCYC 2 cut(s) 418, 1176
Eco47I GGWCC 1 cut(s) 1352
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 3 cut(s) 138, 422, 439
Eco72I CACGTG 1 cut(s) 1076
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 416
EcoO109I RGGNCCY 2 cut(s) 743, 1172
EcoRII CCWGG 2 cut(s) 64, 1168
EcoT14I CCWWGG 4 cut(s) 98, 118, 213, 800
EcoT38I GRGCYC 2 cut(s) 418, 1176
ErhI CCWWGG 4 cut(s) 98, 118, 213, 800
FaeI CATG 5 cut(s) 102, 653, 804, 1120, 1300
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 3 cut(s) 289, 1382, 1520
FatI CATG 5 cut(s) 98, 649, 800, 1116, 1296
FauI CCCGC 3 cut(s) 393, 716, 1417
FbaI TGATCA 1 cut(s) 1389
Fnu4HI GCNGC 3 cut(s) 18, 1185, 1457
FokI GGATG 1 cut(s) 30
FriOI GRGCYC 2 cut(s) 418, 1176
Fsp4HI GCNGC 3 cut(s) 18, 1185, 1457
FspBI CTAG 5 cut(s) 168, 279, 345, 521, 936
GluI GCNGC 3 cut(s) 18, 1185, 1457
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 3 cut(s) 27, 930, 1223
HaeIII GGCC 7 cut(s) 103, 110, 212, 428, 462, 744, 1174
HapII CCGG 3 cut(s) 1008, 1029, 1516
HgaI GACGC 1 cut(s) 867
Hin1II CATG 5 cut(s) 102, 653, 804, 1120, 1300
HincII GTYRAC 1 cut(s) 1071
HindII GTYRAC 1 cut(s) 1071
HinfI GANTC 8 cut(s) 351, 359, 534, 737, 899, 967, 997, 1532
HpaI GTTAAC 1 cut(s) 1071
HpaII CCGG 3 cut(s) 1008, 1029, 1516
HphI GGTGA 3 cut(s) 229, 866, 1541
Hpy166II GTNNAC 7 cut(s) 92, 494, 903, 1071, 1332, 1360, 1496
Hpy188I TCNGA 5 cut(s) 441, 581, 966, 1002, 1411
Hpy188III TCNNGA 3 cut(s) 223, 365, 1117
Hpy8I GTNNAC 7 cut(s) 92, 494, 903, 1071, 1332, 1360, 1496
HpyAV CCTTC 5 cut(s) 202, 446, 832, 1083, 1477
HpyCH4III ACNGT 8 cut(s) 328, 498, 718, 776, 1024, 1317, 1346, 1477
HpyCH4IV ACGT 2 cut(s) 311, 1075
HpyCH4V TGCA 9 cut(s) 20, 81, 290, 479, 941, 1099, 1248, 1436, 1459
HpyF10VI GCNNNNNNNGC 4 cut(s) 459, 857, 1157, 1421
HpyF3I CTNAG 5 cut(s) 448, 615, 965, 1036, 1229
HpySE526I ACGT 2 cut(s) 311, 1075
Hsp92II CATG 5 cut(s) 102, 653, 804, 1120, 1300
Ksp22I TGATCA 1 cut(s) 1389
KspAI GTTAAC 1 cut(s) 1071
Kzo9I GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
LmnI GCTCC 4 cut(s) 413, 802, 833, 1148
Lsp1109I GCAGC 3 cut(s) 4, 1171, 1443
LweI GCATC 3 cut(s) 132, 838, 1468
MaeI CTAG 5 cut(s) 168, 279, 345, 521, 936
MaeII ACGT 2 cut(s) 311, 1075
MaeIII GTNAC 1 cut(s) 1024
MalI GATC 6 cut(s) 221, 578, 660, 689, 1033, 1391
MboI GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
MboII GAAGA 3 cut(s) 1147, 1285, 1383
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 1 cut(s) 1031
MhlI GDGCHC 5 cut(s) 175, 418, 799, 838, 1176
MluCI AATT 4 cut(s) 383, 405, 501, 1142
MlyI GAGTC 3 cut(s) 528, 908, 976
MseI TTAA 4 cut(s) 387, 488, 1070, 1380
MslI CAYNNNNRTG 3 cut(s) 97, 1464, 1591
MspI CCGG 3 cut(s) 1008, 1029, 1516
MspR9I CCNGG 3 cut(s) 66, 1009, 1170
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 1057
MvaI CCWGG 2 cut(s) 66, 1170
MwoI GCNNNNNNNGC 4 cut(s) 459, 857, 1157, 1421
NciI CCSGG 1 cut(s) 1009
NcoI CCATGG 2 cut(s) 98, 800
NdeII GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
NlaIII CATG 5 cut(s) 102, 653, 804, 1120, 1300
NlaIV GGNNCC 3 cut(s) 745, 1033, 1174
NmuCI GTSAC 1 cut(s) 1024
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 1264
PagI TCATGA 1 cut(s) 1116
PaqCI CACCTGC 1 cut(s) 964
PasI CCCWGGG 1 cut(s) 1169
PciI ACATGT 1 cut(s) 649
PctI GAATGC 1 cut(s) 1057
PfeI GAWTC 5 cut(s) 351, 359, 737, 997, 1532
PflFI GACNNNGTC 1 cut(s) 877
PkrI GCNGC 3 cut(s) 19, 1186, 1458
PleI GAGTC 3 cut(s) 528, 907, 975
PmaCI CACGTG 1 cut(s) 1076
PmlI CACGTG 1 cut(s) 1076
PpsI GAGTC 3 cut(s) 528, 907, 975
Ppu21I YACGTR 1 cut(s) 1076
PscI ACATGT 1 cut(s) 649
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 2 cut(s) 64, 1168
PspCI CACGTG 1 cut(s) 1076
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 2 cut(s) 64, 1168
PspN4I GGNNCC 3 cut(s) 745, 1033, 1174
PspOMI GGGCCC 1 cut(s) 1172
PspPI GGNCC 5 cut(s) 102, 743, 1172, 1173, 1352
PstI CTGCAG 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 1 cut(s) 1031
PsyI GACNNNGTC 1 cut(s) 877
RsaI GTAC 3 cut(s) 769, 1319, 1331
RsaNI GTAC 3 cut(s) 768, 1318, 1330
RseI CAYNNNNRTG 3 cut(s) 97, 1464, 1591
SacI GAGCTC 1 cut(s) 418
SaqAI TTAA 4 cut(s) 387, 488, 1070, 1380
SatI GCNGC 3 cut(s) 18, 1185, 1457
Sau3AI GATC 6 cut(s) 219, 576, 658, 687, 1031, 1389
Sau96I GGNCC 5 cut(s) 102, 743, 1172, 1173, 1352
ScaI AGTACT 1 cut(s) 1319
SchI GAGTC 3 cut(s) 528, 908, 976
ScrFI CCNGG 3 cut(s) 66, 1009, 1170
SduI GDGCHC 5 cut(s) 175, 418, 799, 838, 1176
SfaNI GCATC 3 cut(s) 132, 838, 1468
SfcI CTRYAG 3 cut(s) 288, 375, 1044
SfuI TTCGAA 1 cut(s) 1264
SinI GGWCC 1 cut(s) 1352
SmiMI CAYNNNNRTG 3 cut(s) 97, 1464, 1591
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 4 cut(s) 383, 405, 501, 1142
SsiI CCGC 3 cut(s) 400, 723, 1424
SspMI CTAG 5 cut(s) 168, 279, 345, 521, 936
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 3 cut(s) 64, 1007, 1168
StyI CCWWGG 4 cut(s) 98, 118, 213, 800
TaaI ACNGT 8 cut(s) 328, 498, 718, 776, 1024, 1317, 1346, 1477
TaiI ACGT 2 cut(s) 314, 1078
TaqI TCGA 5 cut(s) 27, 349, 1087, 1264, 1462
TasI AATT 4 cut(s) 383, 405, 501, 1142
TatI WGTACW 2 cut(s) 1317, 1329
TfiI GAWTC 5 cut(s) 351, 359, 737, 997, 1532
Tru1I TTAA 4 cut(s) 387, 488, 1070, 1380
Tru9I TTAA 4 cut(s) 387, 488, 1070, 1380
TscAI CASTG 7 cut(s) 297, 361, 463, 501, 721, 910, 954
TseFI GTSAC 1 cut(s) 1024
TseI GCWGC 3 cut(s) 17, 1184, 1456
Tsp45I GTSAC 1 cut(s) 1024
TspDTI ATGAA 5 cut(s) 904, 1105, 1264, 1285, 1545
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 7 cut(s) 297, 361, 463, 501, 721, 910, 954
Tth111I GACNNNGTC 1 cut(s) 877
VpaK11BI GGWCC 1 cut(s) 1352
XapI RAATTY 2 cut(s) 383, 1142
XceI RCATGY 1 cut(s) 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XspI CTAG 5 cut(s) 168, 279, 345, 521, 936
ZrmI AGTACT 1 cut(s) 1319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.