MD06G1226500.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0018276
Physical Location & Seq
Forward (+)
35717852 .. 35720217
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1226500.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGAGAGAGATTCTGCACATCCAGGGAGGGCAATGTGGGAACCAAATAGGAGCAAAGTTCTGGGAGGTTGTGTGCGACGAGCACGGTATAGACCCCACCGGGCAGTACACCGGTAACACCGACATTCAGTTGGAGAGGGTGAATGTTTATTACAACGAGGCCAGCGGCGGCCGTTACGTGCCTCGCGCAGTGCTCATGGACCTCGAGCCAGGCACTATGGACAGCATCCGCGCAGGGCCTTACGGCCAGATTTTCCGACCGGATAACTTTGTTTTTGGCCAGTCCGGGGCCGGAAACAATTGGGCTAAAGGGCATTACACGGAAGGCGCTGAGCTCATTGATTCGGTGCTCGATGTTGTTAGGAAAGAGGCTGAGAATTGTGATTGCTTGCAAGGATTTCAAGTATGTCACTCACTTGGAGGCGGAACCGGTTCTGGAATGGGAACTCTCCTCATTTCAAAGGTAAGAGAAGAATTTCCGGACCGGATGATGCTCACCTTCTCTGTCTTTCCATCTCCGAAGGTTTCAGACACCGTCGTGGAGCCTTACAATGCCACACTTTCCGTTCACCAACTGGTTGAGAATGGAGATGAATGTATGGTCCTCGACAACGAAGCCCTCTACGATATATGCTTCAGGACTCTTAAGCTCACCACCCCAAGCTTTGGGGATTTGAATCATCTGATTTCGGGAACTATGAGCGGAGTTACTTGTTGTTTACGCTTCCCTGGACAATTGAACTCGGATCTGCGAAAGCTAGCCGTGAATTTAATCCCATTCCCTCGACTACACTTCTTCATGGTGGGATTTGCACCTCTGACATCTCGAGGTTCGCAGCAGTACTGCGCATTATCCGTCCCCGAACTGACACAACAAATGTGGGATGCAAAGAATATGATGTGCGCAGCTGATCCGCGCCATGGACGTTACCTCACAGCATCAGCGATGTTTCGAGGCAAAATGAGCACGAAAGAAGTTGACGAACAGATGATGAATGTGCAGAACAAGAACTCCTCCTACTTCGTTGAGTGGATTCCAAACAATGTCAAGTCCAGCGTTTGTGACATTCCACCGCAAGGTCTTATAATGTCTTCGACATTTATTGGAAATTCGACTTCTATACAAGAGATGTTTCGTAGAGTGAGCGAGCAGTTCACGGCAATGTTTAGGAGGAAGGCCTTTTTGCACTGGTATACCGGAGAGGGAATGGATGAGATGGAGTTTACGGAAGCGGAGAGTAACATGAACGATCTTGTTGCGGAATACCAACAGTACCAAGATGCCACGGTTGACGAAGATGGCGATTACGCCGAAGAGGAAGAAGGGATGGGAGGTGGGGAAGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

50.12

Weight (kDa)

4.66

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 211 2.8e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 261 - 382 1.6e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018276)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17280
malus_domestica MD06G1226500.v1.1 MD14G1235900.v1.1
prunus_persica Prupe.5G232700_v2.0.a1
pyrus_communis pycom06g20280
rosa_chinensis RchiOBHm_Chr7g0178491
rosa_laevigata RLG00000005442
rosa_multiflora Rmu_sc0010235.1_g000002
rosa_rugosa Rorug06G0417600
rosa_wichuraiana Rw7G001270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
Acc16I TGCGCA 2 cut(s) 847, 904
AccB7I CCANNNNNTGG 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 702
AccI GTMKAC 1 cut(s) 1193
AccII CGCG 3 cut(s) 186, 231, 916
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 9 cut(s) 165, 168, 229, 423, 702, 914, 1073, 1232, 1259
AclWI GGATC 2 cut(s) 753, 905
AcoI YGGCCR 3 cut(s) 169, 244, 277
AcsI RAATTY 3 cut(s) 473, 766, 1108
AcuI CTGAAG 1 cut(s) 619
AfaI GTAC 3 cut(s) 107, 842, 1274
AfiI CCNNNNNNNGG 4 cut(s) 286, 665, 920, 1076
AflII CTTAAG 1 cut(s) 644
AgeI ACCGGT 2 cut(s) 110, 428
AgsI TTSAA 4 cut(s) 401, 459, 676, 739
AjnI CCWGG 3 cut(s) 21, 208, 727
AleI CACNNNNGTG 1 cut(s) 536
AloI GAACNNNNNNTCC 2 cut(s) 995, 1027
AluBI AGCT 5 cut(s) 334, 649, 663, 757, 908
AluI AGCT 5 cut(s) 334, 649, 663, 757, 908
Alw21I GWGCWC 5 cut(s) 84, 195, 336, 351, 968
AlwI GGATC 2 cut(s) 753, 905
Ama87I CYCGRG 2 cut(s) 203, 825
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 7 cut(s) 159, 169, 236, 244, 277, 288, 1176
ApeKI GCWGC 2 cut(s) 835, 905
ApoI RAATTY 3 cut(s) 473, 766, 1108
AsiGI ACCGGT 2 cut(s) 110, 428
Asp700I GAANNNNTTC 2 cut(s) 430, 474
AspLEI GCGC 6 cut(s) 188, 233, 329, 848, 905, 918
AspS9I GGNCC 5 cut(s) 199, 236, 288, 481, 601
AsuC2I CCSGG 2 cut(s) 100, 286
AsuHPI GGTGA 4 cut(s) 151, 487, 560, 643
AsuNHI GCTAGC 1 cut(s) 757
AvaI CYCGRG 2 cut(s) 203, 825
AvaII GGWCC 3 cut(s) 199, 481, 601
BalI TGGCCA 1 cut(s) 279
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 5 cut(s) 84, 195, 336, 351, 968
BbvI GCAGC 2 cut(s) 847, 917
BccI CCATC 4 cut(s) 520, 1210, 1292, 1321
BceAI ACGGC 4 cut(s) 156, 259, 746, 1173
BcgI CGANNNNNNTGC 2 cut(s) 1238, 1272
BciT130I CCWGG 3 cut(s) 23, 210, 729
BcnI CCSGG 2 cut(s) 100, 286
BfaI CTAG 1 cut(s) 758
BfoI RGCGCY 1 cut(s) 330
BfrI CTTAAG 1 cut(s) 644
BisI GCNGC 4 cut(s) 166, 169, 836, 906
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 4 cut(s) 167, 170, 837, 907
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 5 cut(s) 23, 100, 210, 286, 729
Bme18I GGWCC 3 cut(s) 199, 481, 601
BmeT110I CYCGRG 2 cut(s) 203, 825
BmgT120I GGNCC 5 cut(s) 199, 236, 288, 481, 601
BmiI GGNNCC 4 cut(s) 41, 289, 427, 543
BmrFI CCNGG 5 cut(s) 23, 100, 210, 286, 729
BmsI GCATC 5 cut(s) 234, 480, 874, 947, 1270
BmtI GCTAGC 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 1083
Bpu1102I GCTNAGC 1 cut(s) 330
BpuMI CCSGG 2 cut(s) 100, 286
BsaAI YACGTR 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 5 cut(s) 22, 285, 727, 919, 1284
BsaWI WCCGGW 6 cut(s) 110, 259, 428, 478, 483, 1196
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 995, 1025
Bsc4I CCNNNNNNNGG 4 cut(s) 286, 665, 920, 1076
Bse118I RCCGGY 2 cut(s) 110, 428
Bse1I ACTGG 3 cut(s) 280, 579, 1193
Bse3DI GCAATG 2 cut(s) 38, 1167
Bse8I GATNNNNATC 1 cut(s) 675
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 3 cut(s) 23, 210, 729
BseDI CCNNGG 5 cut(s) 22, 285, 727, 919, 1284
BseGI GGATG 6 cut(s) 18, 225, 492, 889, 1216, 1332
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 4 cut(s) 286, 665, 920, 1076
BseMI GCAATG 2 cut(s) 38, 1167
BseMII CTCAG 2 cut(s) 321, 363
BseNI ACTGG 3 cut(s) 280, 579, 1193
BseRI GAGGAG 2 cut(s) 440, 1003
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 2 cut(s) 847, 917
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 3 cut(s) 186, 231, 916
Bsh1285I CGRYCG 2 cut(s) 172, 260
BshFI GGCC 7 cut(s) 161, 171, 238, 246, 279, 290, 1178
BshTI ACCGGT 2 cut(s) 110, 428
BsiEI CGRYCG 2 cut(s) 172, 260
BsiHKAI GWGCWC 5 cut(s) 84, 195, 336, 351, 968
BsiHKCI CYCGRG 2 cut(s) 203, 825
BsiSI CCGG 9 cut(s) 99, 111, 260, 285, 291, 429, 479, 484, 1197
BslFI GGGAC 1 cut(s) 842
BslI CCNNNNNNNGG 4 cut(s) 286, 665, 920, 1076
BsmFI GGGAC 1 cut(s) 842
BsnI GGCC 7 cut(s) 161, 171, 238, 246, 279, 290, 1178
BsoBI CYCGRG 2 cut(s) 203, 825
Bsp1286I GDGCHC 5 cut(s) 84, 195, 336, 351, 968
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 3 cut(s) 745, 910, 1249
Bsp1720I GCTNAGC 1 cut(s) 330
Bsp19I CCATGG 1 cut(s) 919
BspACI CCGC 9 cut(s) 165, 168, 229, 423, 702, 914, 1073, 1232, 1259
BspANI GGCC 7 cut(s) 161, 171, 238, 246, 279, 290, 1178
BspCNI CTCAG 2 cut(s) 322, 364
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 3 cut(s) 186, 231, 916
BspLI GGNNCC 4 cut(s) 41, 289, 427, 543
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 2 cut(s) 753, 905
BspTI CTTAAG 1 cut(s) 644
BsrBI CCGCTC 1 cut(s) 702
BsrDI GCAATG 2 cut(s) 38, 1167
BsrFI RCCGGY 2 cut(s) 110, 428
BsrI ACTGG 3 cut(s) 280, 579, 1193
BssAI RCCGGY 2 cut(s) 110, 428
BssECI CCNNGG 5 cut(s) 22, 285, 727, 919, 1284
BssMI GATC 3 cut(s) 745, 910, 1249
BssNAI GTATAC 1 cut(s) 1194
BssT1I CCWWGG 1 cut(s) 919
Bst1107I GTATAC 1 cut(s) 1194
Bst2UI CCWGG 3 cut(s) 23, 210, 729
Bst4CI ACNGT 4 cut(s) 86, 535, 1272, 1288
Bst6I CTCTTC 1 cut(s) 1308
BstAFI CTTAAG 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 4 cut(s) 163, 389, 759, 1148
BstDEI CTNAG 2 cut(s) 330, 372
BstDSI CCRYGG 2 cut(s) 919, 1284
BstF5I GGATG 6 cut(s) 18, 225, 492, 889, 1216, 1332
BstFNI CGCG 3 cut(s) 186, 231, 916
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 6 cut(s) 188, 233, 329, 848, 905, 918
BstKTI GATC 3 cut(s) 748, 913, 1252
BstMBI GATC 3 cut(s) 745, 910, 1249
BstMCI CGRYCG 2 cut(s) 172, 260
BstMWI GCNNNNNNNGC 1 cut(s) 963
BstNI CCWGG 3 cut(s) 23, 210, 729
BstSCI CCNGG 5 cut(s) 21, 98, 208, 284, 727
BstUI CGCG 3 cut(s) 186, 231, 916
BstV1I GCAGC 2 cut(s) 847, 917
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
BstZ17I GTATAC 1 cut(s) 1194
BstZI CGGCCG 1 cut(s) 169
BsuRI GGCC 7 cut(s) 161, 171, 238, 246, 279, 290, 1178
BtgI CCRYGG 2 cut(s) 919, 1284
BtgZI GCGATG 1 cut(s) 959
BtsCI GGATG 6 cut(s) 18, 225, 492, 889, 1216, 1332
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 1186
Cac8I GCNNGC 4 cut(s) 163, 389, 759, 1148
CfoI GCGC 6 cut(s) 188, 233, 329, 848, 905, 918
Cfr10I RCCGGY 2 cut(s) 110, 428
Cfr13I GGNCC 5 cut(s) 199, 236, 288, 481, 601
CpoI CGGWCCG 1 cut(s) 481
Csp6I GTAC 3 cut(s) 106, 841, 1273
CspAI ACCGGT 2 cut(s) 110, 428
CspCI CAANNNNNGTGG 2 cut(s) 860, 895
CspI CGGWCCG 1 cut(s) 481
CviAII CATG 4 cut(s) 196, 799, 920, 1243
CviQI GTAC 3 cut(s) 106, 841, 1273
DdeI CTNAG 2 cut(s) 330, 372
DpnI GATC 3 cut(s) 747, 912, 1251
DpnII GATC 3 cut(s) 745, 910, 1249
EaeI YGGCCR 3 cut(s) 169, 244, 277
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 1308
EarI CTCTTC 1 cut(s) 1308
EciI GGCGGA 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 334
EclXI CGGCCG 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 919
Eco147I AGGCCT 1 cut(s) 1178
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 3 cut(s) 199, 481, 601
Eco52I CGGCCG 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 619
Eco88I CYCGRG 2 cut(s) 203, 825
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 3 cut(s) 21, 208, 727
EcoT14I CCWWGG 1 cut(s) 919
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 919
FaeI CATG 4 cut(s) 199, 802, 923, 1246
FaqI GGGAC 1 cut(s) 842
FatI CATG 4 cut(s) 195, 798, 919, 1242
FblI GTMKAC 1 cut(s) 1193
Fnu4HI GCNGC 4 cut(s) 166, 169, 836, 906
FokI GGATG 6 cut(s) 5, 212, 499, 896, 1223, 1339
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 4 cut(s) 166, 169, 836, 906
FspBI CTAG 1 cut(s) 758
FspI TGCGCA 2 cut(s) 847, 904
GlaI GCGC 6 cut(s) 187, 232, 328, 847, 904, 917
GluI GCNGC 4 cut(s) 166, 169, 836, 906
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 7 cut(s) 161, 171, 238, 246, 279, 290, 1178
HapII CCGG 9 cut(s) 99, 111, 260, 285, 291, 429, 479, 484, 1197
HhaI GCGC 6 cut(s) 188, 233, 329, 848, 905, 918
Hin1II CATG 4 cut(s) 199, 802, 923, 1246
Hin6I GCGC 6 cut(s) 186, 231, 327, 846, 903, 916
HinP1I GCGC 6 cut(s) 186, 231, 327, 846, 903, 916
HincII GTYRAC 2 cut(s) 979, 1291
HindII GTYRAC 2 cut(s) 979, 1291
HindIII AAGCTT 1 cut(s) 661
HinfI GANTC 5 cut(s) 10, 341, 640, 676, 1033
HpaII CCGG 9 cut(s) 99, 111, 260, 285, 291, 429, 479, 484, 1197
HphI GGTGA 4 cut(s) 151, 487, 560, 643
Hpy166II GTNNAC 8 cut(s) 108, 568, 719, 979, 1155, 1194, 1224, 1291
Hpy188I TCNGA 7 cut(s) 257, 519, 529, 684, 745, 819, 1349
Hpy188III TCNNGA 5 cut(s) 435, 479, 637, 690, 825
Hpy8I GTNNAC 8 cut(s) 108, 568, 719, 979, 1155, 1194, 1224, 1291
Hpy99I CGWCG 2 cut(s) 80, 539
HpyAV CCTTC 5 cut(s) 317, 508, 514, 1168, 1316
HpyCH4III ACNGT 4 cut(s) 86, 535, 1272, 1288
HpyCH4IV ACGT 2 cut(s) 177, 925
HpyCH4V TGCA 6 cut(s) 16, 391, 812, 887, 1000, 1186
HpyF10VI GCNNNNNNNGC 1 cut(s) 963
HpyF3I CTNAG 2 cut(s) 330, 372
HpySE526I ACGT 2 cut(s) 177, 925
Hsp92II CATG 4 cut(s) 199, 802, 923, 1246
HspAI GCGC 6 cut(s) 186, 231, 327, 846, 903, 916
Kpn2I TCCGGA 1 cut(s) 478
Kzo9I GATC 3 cut(s) 745, 910, 1249
LmnI GCTCC 2 cut(s) 50, 541
Lsp1109I GCAGC 2 cut(s) 847, 917
LweI GCATC 5 cut(s) 234, 480, 874, 947, 1270
MaeI CTAG 1 cut(s) 758
MaeII ACGT 2 cut(s) 177, 925
MaeIII GTNAC 7 cut(s) 113, 173, 407, 706, 926, 1061, 1238
MalI GATC 3 cut(s) 747, 912, 1251
MbiI CCGCTC 1 cut(s) 702
MboI GATC 3 cut(s) 745, 910, 1249
MboII GAAGA 6 cut(s) 482, 787, 1083, 1307, 1325, 1331
MfeI CAATTG 2 cut(s) 298, 734
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 5 cut(s) 84, 195, 336, 351, 968
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 6 cut(s) 298, 376, 473, 734, 766, 1108
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 1 cut(s) 634
MmeI TCCRAC 2 cut(s) 111, 280
Mox20I TGGCCA 1 cut(s) 279
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 2 cut(s) 430, 474
MscI TGGCCA 1 cut(s) 279
MseI TTAA 2 cut(s) 645, 770
MslI CAYNNNNRTG 2 cut(s) 536, 1160
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 165, 908
MspCI CTTAAG 1 cut(s) 644
MspI CCGG 9 cut(s) 99, 111, 260, 285, 291, 429, 479, 484, 1197
MspR9I CCNGG 5 cut(s) 23, 100, 210, 286, 729
MunI CAATTG 2 cut(s) 298, 734
MvaI CCWGG 3 cut(s) 23, 210, 729
MvnI CGCG 3 cut(s) 186, 231, 916
MwoI GCNNNNNNNGC 1 cut(s) 963
NciI CCSGG 2 cut(s) 100, 286
NcoI CCATGG 1 cut(s) 919
NdeII GATC 3 cut(s) 745, 910, 1249
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 4 cut(s) 199, 802, 923, 1246
NlaIV GGNNCC 4 cut(s) 41, 289, 427, 543
NmuCI GTSAC 2 cut(s) 407, 1061
NsbI TGCGCA 2 cut(s) 847, 904
OliI CACNNNNGTG 1 cut(s) 536
PaeR7I CTCGAG 2 cut(s) 203, 825
PceI AGGCCT 1 cut(s) 1178
PcsI WCGNNNNNNNCGW 1 cut(s) 1299
PdmI GAANNNNTTC 2 cut(s) 430, 474
PfeI GAWTC 4 cut(s) 10, 341, 676, 1033
PflFI GACNNNGTC 1 cut(s) 533
PflMI CCANNNNNTGG 1 cut(s) 665
PinAI ACCGGT 2 cut(s) 110, 428
PkrI GCNGC 4 cut(s) 167, 170, 837, 907
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
Ppu21I YACGTR 1 cut(s) 178
PsiI TTATAA 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 21, 208, 727
PspGI CCWGG 3 cut(s) 21, 208, 727
PspN4I GGNNCC 4 cut(s) 41, 289, 427, 543
PspPI GGNCC 5 cut(s) 199, 236, 288, 481, 601
PspXI VCTCGAGB 1 cut(s) 203
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PsuI RGATCY 1 cut(s) 745
PsyI GACNNNGTC 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 3 cut(s) 107, 842, 1274
RsaNI GTAC 3 cut(s) 106, 841, 1273
RseI CAYNNNNRTG 2 cut(s) 536, 1160
Rsr2I CGGWCCG 1 cut(s) 481
RsrII CGGWCCG 1 cut(s) 481
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 645, 770
SatI GCNGC 4 cut(s) 166, 169, 836, 906
Sau3AI GATC 3 cut(s) 745, 910, 1249
Sau96I GGNCC 5 cut(s) 199, 236, 288, 481, 601
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 1 cut(s) 634
ScrFI CCNGG 5 cut(s) 23, 100, 210, 286, 729
SduI GDGCHC 5 cut(s) 84, 195, 336, 351, 968
SfaNI GCATC 5 cut(s) 234, 480, 874, 947, 1270
Sfr274I CTCGAG 2 cut(s) 203, 825
SinI GGWCC 3 cut(s) 199, 481, 601
SlaI CTCGAG 2 cut(s) 203, 825
SmiMI CAYNNNNRTG 2 cut(s) 536, 1160
SmlI CTYRAG 3 cut(s) 203, 644, 825
SmoI CTYRAG 3 cut(s) 203, 644, 825
Sse9I AATT 6 cut(s) 298, 376, 473, 734, 766, 1108
SseBI AGGCCT 1 cut(s) 1178
SsiI CCGC 9 cut(s) 165, 168, 229, 423, 702, 914, 1073, 1232, 1259
SspMI CTAG 1 cut(s) 758
SstI GAGCTC 1 cut(s) 336
StuI AGGCCT 1 cut(s) 1178
StyD4I CCNGG 5 cut(s) 21, 98, 208, 284, 727
StyI CCWWGG 1 cut(s) 919
TaaI ACNGT 4 cut(s) 86, 535, 1272, 1288
TaiI ACGT 2 cut(s) 180, 928
TaqI TCGA 8 cut(s) 204, 351, 606, 784, 826, 952, 1094, 1112
TasI AATT 6 cut(s) 298, 376, 473, 734, 766, 1108
TatI WGTACW 2 cut(s) 105, 840
TauI GCSGC 2 cut(s) 168, 171
TfiI GAWTC 4 cut(s) 10, 341, 676, 1033
Tru1I TTAA 2 cut(s) 645, 770
Tru9I TTAA 2 cut(s) 645, 770
TscAI CASTG 2 cut(s) 195, 1193
TseFI GTSAC 2 cut(s) 407, 1061
TseI GCWGC 2 cut(s) 835, 905
Tsp45I GTSAC 2 cut(s) 407, 1061
TspDTI ATGAA 4 cut(s) 606, 787, 1007, 1259
TspGWI ACGGA 4 cut(s) 335, 553, 844, 1241
TspRI CASTG 2 cut(s) 195, 1193
Tth111I GACNNNGTC 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 665
Vha464I CTTAAG 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 199, 481, 601
XapI RAATTY 3 cut(s) 473, 766, 1108
XhoI CTCGAG 2 cut(s) 203, 825
XmiI GTMKAC 1 cut(s) 1193
XmnI GAANNNNTTC 2 cut(s) 430, 474
XspI CTAG 1 cut(s) 758
ZrmI AGTACT 1 cut(s) 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.