Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0018276

Physical Location & Seq

Genomic Coordinates Forward (+)

31586811 .. 31589183

Gene Structure 2373 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1235900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGAGAGAGATTCTGCACATCCAGGGAGGGCAATGTGGGAACCAAATAGGATCAAAGTTCTGGGAGGTTGTGTGCGACGAGCACGGCATAGACCCCACCGGGCAGTACACCGGTAACACTGACATTCAGTTGGAGAGGGTCAATGTTTATTACAACGAGGCCAGCGGCGGCCGTTACGTGCCTCGCGCGGTGCTCATGGACCTCGAGCCAGGCACCATGGACAGCATTCGCGCTGGGCCTTACGGCCAGATTTTCCGGCCAGATAACTTTGTTTTTGGACAGTCCGGGGCCGGAAACAATTGGGCTAAAGGTCATTACACGGAAGGCGCTGAGCTTATTGATTCGGTGCTCGATGTTGTTAGGAAAGAGGCTGAGAGCTGTGACTGCTTGCAAGGATTTCAAGTATGTCACTCACTTGGAGGCGGAACCGGCTCCGGAATGGGAACTCTGCTCATTTCAAAGATCAGAGAAGAATTCCCAGATCGAATGATGCTCACCTTCTCTGTCTTTCCATCTCCGAAGGTTTCAGACACTGTTGTAGAGCCTTACAATGCCACACTTTCCGTTCACCAACTGGTCGAGAATGGCGATGAGTGTATGGTCCTCGACAATGAAGCCCTCTATGATATCTGCTTTAGGACTCTCAAGCTCACCACCCCAAGCTTTGGGGATCTGAATCATCTGATTTCGGGAACTATGAGCGGAGTTACTTGTTGCCTACGCTTCCCTGGACAATTGAACTCAGATCTGCGAAAGCTAGCCGTGAACTTAATCCCATTCCCACGACTACACTTCTTCATGGTGGGATTTGCGCCTCTGACATCTCGAGGATCGCAGCAGTACTGTGCATTGTCTGTCCCCGAACTGACACAACAAATGTGGGATGCCAAAAACATGATGTGCGCAGCTGATCCGCGCCACGGACGTTACCTCACAGCATCAGCGATGTTTCGAGGTAAAATGAGCACAAAAGAAGTAGACGAACAGATGATGAATGTGCAGAACAAGAACTCCTCCTACTTCGTTGAATGGATTCCGAACAATGTGAAATCCAGCGTTTGTGACATTCCGCCGCAAGGTCTTATCATGTCTTCGACATTCATTGGGAATTCGACTTCTATACAAGAGATGTTCCGAAGAGTAAGCGAGCAGTTCACGGCGATGTTTAGGAGGAAGGCCTTCTTGCACTGGTATACCGGAGAAGGAATGGATGAGATGGAGTTTACGGAAGCGGAGAGCAACATGAACGATCTTGTTGCGGAATACCAACAGTACCAAGATGCCACGATTGATGAAGATGGCGATTACGTCGAAGAGGAAGAAGGGATGGGAGGTGGAGAAGAACACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04145 ko04540 ko05130 map04145 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

50.19

Weight (kDa)

4.7

Isoelectric Point (pI)

37.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 211	1.1e-63	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	261 - 382	1.6e-39	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0018276)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g17280
malus_domestica	MD06G1226500.v1.1 MD14G1235900.v1.1
prunus_persica	Prupe.5G232700_v2.0.a1
pyrus_communis	pycom06g20280
rosa_chinensis	RchiOBHm_Chr7g0178491
rosa_laevigata	RLG00000005442
rosa_multiflora	Rmu_sc0010235.1_g000002
rosa_rugosa	Rorug06G0417600
rosa_wichuraiana	Rw7G001270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	904
AccB1I	GGYRCC	1 cut(s)	212
AccB7I	CCANNNNNTGG	1 cut(s)	665
AccBSI	CCGCTC	1 cut(s)	702
AccI	GTMKAC	2 cut(s)	978, 1193
AccII	CGCG	4 cut(s)	186, 188, 231, 916
AccIII	TCCGGA	1 cut(s)	434
AclWI	GGATC	4 cut(s)	58, 678, 838, 905
AcoI	YGGCCR	3 cut(s)	169, 244, 257
AcsI	RAATTY	2 cut(s)	473, 1108
AfaI	GTAC	3 cut(s)	107, 842, 1274
AfiI	CCNNNNNNNGG	3 cut(s)	665, 920, 1076
AgeI	ACCGGT	1 cut(s)	110
AgsI	TTSAA	4 cut(s)	401, 459, 739, 1028
AjnI	CCWGG	3 cut(s)	21, 208, 727
AloI	GAACNNNNNNTCC	2 cut(s)	995, 1027
AluBI	AGCT	6 cut(s)	334, 378, 649, 663, 757, 908
AluI	AGCT	6 cut(s)	334, 378, 649, 663, 757, 908
Alw21I	GWGCWC	4 cut(s)	84, 195, 351, 968
AlwI	GGATC	4 cut(s)	58, 678, 838, 905
AlwNI	CAGNNNCTG	1 cut(s)	533
Ama87I	CYCGRG	2 cut(s)	203, 825
Aor13HI	TCCGGA	1 cut(s)	434
AoxI	GGCC	7 cut(s)	159, 169, 236, 244, 257, 288, 1176
ApeKI	GCWGC	2 cut(s)	835, 905
ApoI	RAATTY	2 cut(s)	473, 1108
AsiGI	ACCGGT	1 cut(s)	110
Asp700I	GAANNNNTTC	2 cut(s)	1032, 1178
AspLEI	GCGC	6 cut(s)	188, 233, 329, 814, 905, 918
AspS9I	GGNCC	4 cut(s)	199, 236, 288, 601
AsuC2I	CCSGG	2 cut(s)	100, 286
AsuHPI	GGTGA	3 cut(s)	487, 560, 643
AsuNHI	GCTAGC	1 cut(s)	757
AvaI	CYCGRG	2 cut(s)	203, 825
AvaII	GGWCC	2 cut(s)	199, 601
BanI	GGYRCC	1 cut(s)	212
BbsI	GAAGAC	1 cut(s)	1083
Bbv12I	GWGCWC	4 cut(s)	84, 195, 351, 968
BbvI	GCAGC	2 cut(s)	847, 917
BccI	CCATC	4 cut(s)	520, 1210, 1292, 1321
BceAI	ACGGC	5 cut(s)	100, 156, 259, 746, 1173
BcgI	CGANNNNNNTGC	2 cut(s)	1238, 1272
BciT130I	CCWGG	3 cut(s)	23, 210, 729
BcnI	CCSGG	2 cut(s)	100, 286
BfaI	CTAG	1 cut(s)	758
BfoI	RGCGCY	1 cut(s)	330
BglII	AGATCT	1 cut(s)	745
BisI	GCNGC	5 cut(s)	166, 169, 836, 906, 1073
BlpI	GCTNAGC	1 cut(s)	330
BlsI	GCNGC	5 cut(s)	167, 170, 837, 907, 1074
BmcAI	AGTACT	1 cut(s)	842
Bme1390I	CCNGG	5 cut(s)	23, 100, 210, 286, 729
Bme18I	GGWCC	2 cut(s)	199, 601
BmeT110I	CYCGRG	2 cut(s)	203, 825
BmgT120I	GGNCC	4 cut(s)	199, 236, 288, 601
BmiI	GGNNCC	5 cut(s)	41, 214, 289, 427, 433
BmrFI	CCNGG	5 cut(s)	23, 100, 210, 286, 729
BmsI	GCATC	4 cut(s)	480, 874, 947, 1270
BmtI	GCTAGC	1 cut(s)	761
BpiI	GAAGAC	1 cut(s)	1083
Bpu1102I	GCTNAGC	1 cut(s)	330
BpuEI	CTTGAG	1 cut(s)	629
BpuMI	CCSGG	2 cut(s)	100, 286
BsaAI	YACGTR	1 cut(s)	178
BsaBI	GATNNNNATC	1 cut(s)	675
BsaJI	CCNNGG	5 cut(s)	22, 216, 285, 727, 919
BsaWI	WCCGGW	3 cut(s)	110, 434, 1196
BsaXI	ACNNNNNCTCC	4 cut(s)	56, 86, 995, 1025
Bsc4I	CCNNNNNNNGG	3 cut(s)	665, 920, 1076
Bse118I	RCCGGY	2 cut(s)	110, 428
Bse1I	ACTGG	2 cut(s)	579, 1193
Bse3DI	GCAATG	1 cut(s)	38
Bse8I	GATNNNNATC	1 cut(s)	675
BseAI	TCCGGA	1 cut(s)	434
BseBI	CCWGG	3 cut(s)	23, 210, 729
BseDI	CCNNGG	5 cut(s)	22, 216, 285, 727, 919
BseGI	GGATG	4 cut(s)	18, 889, 1216, 1332
BseJI	GATNNNNATC	1 cut(s)	675
BseLI	CCNNNNNNNGG	3 cut(s)	665, 920, 1076
BseMI	GCAATG	1 cut(s)	38
BseMII	CTCAG	3 cut(s)	321, 363, 756
BseNI	ACTGG	2 cut(s)	579, 1193
BseRI	GAGGAG	1 cut(s)	1003
BseX3I	CGGCCG	1 cut(s)	169
BseXI	GCAGC	2 cut(s)	847, 917
BseYI	CCCAGC	1 cut(s)	233
BsgI	GTGCAG	1 cut(s)	1019
Bsh1236I	CGCG	4 cut(s)	186, 188, 231, 916
Bsh1285I	CGRYCG	1 cut(s)	172
BshFI	GGCC	7 cut(s)	161, 171, 238, 246, 259, 290, 1178
BshNI	GGYRCC	1 cut(s)	212
BshTI	ACCGGT	1 cut(s)	110
BsiEI	CGRYCG	1 cut(s)	172
BsiHKAI	GWGCWC	4 cut(s)	84, 195, 351, 968
BsiHKCI	CYCGRG	2 cut(s)	203, 825
BsiSI	CCGG	8 cut(s)	99, 111, 256, 285, 291, 429, 435, 1197
BslFI	GGGAC	1 cut(s)	842
BslI	CCNNNNNNNGG	3 cut(s)	665, 920, 1076
BsmFI	GGGAC	1 cut(s)	842
BsmI	GAATGC	1 cut(s)	225
BsnI	GGCC	7 cut(s)	161, 171, 238, 246, 259, 290, 1178
BsoBI	CYCGRG	2 cut(s)	203, 825
Bsp1286I	GDGCHC	4 cut(s)	84, 195, 351, 968
Bsp13I	TCCGGA	1 cut(s)	434
Bsp143I	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
Bsp1720I	GCTNAGC	1 cut(s)	330
Bsp19I	CCATGG	1 cut(s)	216
BspANI	GGCC	7 cut(s)	161, 171, 238, 246, 259, 290, 1178
BspCNI	CTCAG	3 cut(s)	322, 364, 755
BspEI	TCCGGA	1 cut(s)	434
BspFNI	CGCG	4 cut(s)	186, 188, 231, 916
BspLI	GGNNCC	5 cut(s)	41, 214, 289, 427, 433
BspOI	GCTAGC	1 cut(s)	761
BspPI	GGATC	4 cut(s)	58, 678, 838, 905
BspT107I	GGYRCC	1 cut(s)	212
BsrBI	CCGCTC	1 cut(s)	702
BsrDI	GCAATG	1 cut(s)	38
BsrFI	RCCGGY	2 cut(s)	110, 428
BsrI	ACTGG	2 cut(s)	579, 1193
BssAI	RCCGGY	2 cut(s)	110, 428
BssECI	CCNNGG	5 cut(s)	22, 216, 285, 727, 919
BssMI	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
BssNAI	GTATAC	1 cut(s)	1194
BssT1I	CCWWGG	1 cut(s)	216
Bst1107I	GTATAC	1 cut(s)	1194
Bst2UI	CCWGG	3 cut(s)	23, 210, 729
Bst4CI	ACNGT	4 cut(s)	282, 535, 845, 1272
Bst6I	CTCTTC	2 cut(s)	1132, 1308
BstBAI	YACGTR	1 cut(s)	178
BstC8I	GCNNGC	4 cut(s)	163, 389, 759, 1148
BstDEI	CTNAG	3 cut(s)	330, 372, 742
BstDSI	CCRYGG	2 cut(s)	216, 919
BstF5I	GGATG	4 cut(s)	18, 889, 1216, 1332
BstFNI	CGCG	4 cut(s)	186, 188, 231, 916
BstH2I	RGCGCY	1 cut(s)	330
BstHHI	GCGC	6 cut(s)	188, 233, 329, 814, 905, 918
BstKTI	GATC	8 cut(s)	53, 465, 484, 673, 748, 833, 913, 1252
BstMBI	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
BstMCI	CGRYCG	1 cut(s)	172
BstMWI	GCNNNNNNNGC	2 cut(s)	384, 429
BstNI	CCWGG	3 cut(s)	23, 210, 729
BstSCI	CCNGG	5 cut(s)	21, 98, 208, 284, 727
BstUI	CGCG	4 cut(s)	186, 188, 231, 916
BstV1I	GCAGC	2 cut(s)	847, 917
BstV2I	GAAGAC	1 cut(s)	1083
BstX2I	RGATCY	2 cut(s)	670, 745
BstYI	RGATCY	2 cut(s)	670, 745
BstZ17I	GTATAC	1 cut(s)	1194
BstZI	CGGCCG	1 cut(s)	169
BsuRI	GGCC	7 cut(s)	161, 171, 238, 246, 259, 290, 1178
BtgI	CCRYGG	2 cut(s)	216, 919
BtgZI	GCGATG	3 cut(s)	603, 959, 1175
BtsCI	GGATG	4 cut(s)	18, 889, 1216, 1332
BtsIMutI	CAGTG	4 cut(s)	117, 531, 1186, 1345
Cac8I	GCNNGC	4 cut(s)	163, 389, 759, 1148
CaiI	CAGNNNCTG	1 cut(s)	533
CfoI	GCGC	6 cut(s)	188, 233, 329, 814, 905, 918
Cfr10I	RCCGGY	2 cut(s)	110, 428
Cfr13I	GGNCC	4 cut(s)	199, 236, 288, 601
Csp6I	GTAC	3 cut(s)	106, 841, 1273
CspAI	ACCGGT	1 cut(s)	110
CspCI	CAANNNNNGTGG	2 cut(s)	860, 895
CviAII	CATG	6 cut(s)	196, 217, 799, 895, 1087, 1243
CviQI	GTAC	3 cut(s)	106, 841, 1273
DdeI	CTNAG	3 cut(s)	330, 372, 742
DpnI	GATC	8 cut(s)	52, 464, 483, 672, 747, 832, 912, 1251
DpnII	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
EaeI	YGGCCR	3 cut(s)	169, 244, 257
EagI	CGGCCG	1 cut(s)	169
Eam1104I	CTCTTC	2 cut(s)	1132, 1308
EarI	CTCTTC	2 cut(s)	1132, 1308
EciI	GGCGGA	2 cut(s)	438, 1059
EclXI	CGGCCG	1 cut(s)	169
Eco130I	CCWWGG	1 cut(s)	216
Eco147I	AGGCCT	1 cut(s)	1178
Eco32I	GATATC	1 cut(s)	628
Eco47I	GGWCC	2 cut(s)	199, 601
Eco52I	CGGCCG	1 cut(s)	169
Eco88I	CYCGRG	2 cut(s)	203, 825
EcoRI	GAATTC	2 cut(s)	473, 1108
EcoRII	CCWGG	3 cut(s)	21, 208, 727
EcoRV	GATATC	1 cut(s)	628
EcoT14I	CCWWGG	1 cut(s)	216
ErhI	CCWWGG	1 cut(s)	216
FaeI	CATG	6 cut(s)	199, 220, 802, 898, 1090, 1246
FalI	AAGNNNNNCTT	2 cut(s)	1166, 1198
FaqI	GGGAC	1 cut(s)	842
FatI	CATG	6 cut(s)	195, 216, 798, 894, 1086, 1242
FblI	GTMKAC	2 cut(s)	978, 1193
Fnu4HI	GCNGC	5 cut(s)	166, 169, 836, 906, 1073
FokI	GGATG	4 cut(s)	5, 896, 1223, 1339
Fsp4HI	GCNGC	5 cut(s)	166, 169, 836, 906, 1073
FspBI	CTAG	1 cut(s)	758
FspI	TGCGCA	1 cut(s)	904
GlaI	GCGC	6 cut(s)	187, 232, 328, 813, 904, 917
GluI	GCNGC	5 cut(s)	166, 169, 836, 906, 1073
GsaI	CCCAGC	1 cut(s)	237
HaeII	RGCGCY	1 cut(s)	330
HaeIII	GGCC	7 cut(s)	161, 171, 238, 246, 259, 290, 1178
HapII	CCGG	8 cut(s)	99, 111, 256, 285, 291, 429, 435, 1197
HhaI	GCGC	6 cut(s)	188, 233, 329, 814, 905, 918
Hin1II	CATG	6 cut(s)	199, 220, 802, 898, 1090, 1246
Hin6I	GCGC	6 cut(s)	186, 231, 327, 812, 903, 916
HinP1I	GCGC	6 cut(s)	186, 231, 327, 812, 903, 916
HindIII	AAGCTT	1 cut(s)	661
HinfI	GANTC	5 cut(s)	10, 341, 640, 676, 1033
HpaII	CCGG	8 cut(s)	99, 111, 256, 285, 291, 429, 435, 1197
HphI	GGTGA	3 cut(s)	487, 560, 643
Hpy166II	GTNNAC	7 cut(s)	108, 568, 766, 979, 1155, 1194, 1224
Hpy188I	TCNGA	9 cut(s)	467, 519, 529, 675, 684, 745, 819, 1038, 1136
Hpy188III	TCNNGA	4 cut(s)	435, 580, 690, 825
Hpy8I	GTNNAC	7 cut(s)	108, 568, 766, 979, 1155, 1194, 1224
Hpy99I	CGWCG	2 cut(s)	80, 1313
HpyAV	CCTTC	7 cut(s)	317, 508, 514, 1168, 1189, 1196, 1316
HpyCH4III	ACNGT	4 cut(s)	282, 535, 845, 1272
HpyCH4IV	ACGT	3 cut(s)	177, 925, 1308
HpyCH4V	TGCA	5 cut(s)	16, 391, 848, 1000, 1186
HpyF10VI	GCNNNNNNNGC	2 cut(s)	384, 429
HpyF3I	CTNAG	3 cut(s)	330, 372, 742
HpySE526I	ACGT	3 cut(s)	177, 925, 1308
Hsp92II	CATG	6 cut(s)	199, 220, 802, 898, 1090, 1246
HspAI	GCGC	6 cut(s)	186, 231, 327, 812, 903, 916
Kpn2I	TCCGGA	1 cut(s)	434
Kzo9I	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
LmnI	GCTCC	1 cut(s)	437
Lsp1109I	GCAGC	2 cut(s)	847, 917
LweI	GCATC	4 cut(s)	480, 874, 947, 1270
MaeI	CTAG	1 cut(s)	758
MaeII	ACGT	3 cut(s)	177, 925, 1308
MaeIII	GTNAC	7 cut(s)	113, 173, 380, 407, 706, 926, 1061
MalI	GATC	8 cut(s)	52, 464, 483, 672, 747, 832, 912, 1251
MbiI	CCGCTC	1 cut(s)	702
MboI	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
MboII	GAAGA	7 cut(s)	482, 787, 1083, 1149, 1307, 1325, 1331
MfeI	CAATTG	2 cut(s)	298, 734
MflI	RGATCY	2 cut(s)	670, 745
MhlI	GDGCHC	4 cut(s)	84, 195, 351, 968
MluCI	AATT	4 cut(s)	298, 473, 734, 1108
MlyI	GAGTC	1 cut(s)	634
MmeI	TCCRAC	1 cut(s)	111
MroI	TCCGGA	1 cut(s)	434
MroXI	GAANNNNTTC	2 cut(s)	1032, 1178
MseI	TTAA	1 cut(s)	770
MslI	CAYNNNNRTG	1 cut(s)	1160
MspA1I	CMGCKG	2 cut(s)	165, 908
MspI	CCGG	8 cut(s)	99, 111, 256, 285, 291, 429, 435, 1197
MspR9I	CCNGG	5 cut(s)	23, 100, 210, 286, 729
MunI	CAATTG	2 cut(s)	298, 734
Mva1269I	GAATGC	1 cut(s)	225
MvaI	CCWGG	3 cut(s)	23, 210, 729
MvnI	CGCG	4 cut(s)	186, 188, 231, 916
MwoI	GCNNNNNNNGC	2 cut(s)	384, 429
NciI	CCSGG	2 cut(s)	100, 286
NcoI	CCATGG	1 cut(s)	216
NdeII	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
NheI	GCTAGC	1 cut(s)	757
NlaIII	CATG	6 cut(s)	199, 220, 802, 898, 1090, 1246
NlaIV	GGNNCC	5 cut(s)	41, 214, 289, 427, 433
NmuCI	GTSAC	3 cut(s)	380, 407, 1061
NsbI	TGCGCA	1 cut(s)	904
PaeR7I	CTCGAG	2 cut(s)	203, 825
PceI	AGGCCT	1 cut(s)	1178
PcsI	WCGNNNNNNNCGW	1 cut(s)	585
PctI	GAATGC	1 cut(s)	225
PdmI	GAANNNNTTC	2 cut(s)	1032, 1178
PfeI	GAWTC	4 cut(s)	10, 341, 676, 1033
PflMI	CCANNNNNTGG	1 cut(s)	665
PinAI	ACCGGT	1 cut(s)	110
PkrI	GCNGC	5 cut(s)	167, 170, 837, 907, 1074
PleI	GAGTC	1 cut(s)	634
PpsI	GAGTC	1 cut(s)	634
Ppu21I	YACGTR	1 cut(s)	178
Psp6I	CCWGG	3 cut(s)	21, 208, 727
PspFI	CCCAGC	1 cut(s)	233
PspGI	CCWGG	3 cut(s)	21, 208, 727
PspN4I	GGNNCC	5 cut(s)	41, 214, 289, 427, 433
PspPI	GGNCC	4 cut(s)	199, 236, 288, 601
PspXI	VCTCGAGB	1 cut(s)	203
PsrI	GAACNNNNNNTAC	2 cut(s)	1001, 1033
PstNI	CAGNNNCTG	1 cut(s)	533
PsuI	RGATCY	2 cut(s)	670, 745
PvuII	CAGCTG	1 cut(s)	908
RsaI	GTAC	3 cut(s)	107, 842, 1274
RsaNI	GTAC	3 cut(s)	106, 841, 1273
RseI	CAYNNNNRTG	1 cut(s)	1160
SaqAI	TTAA	1 cut(s)	770
SatI	GCNGC	5 cut(s)	166, 169, 836, 906, 1073
Sau3AI	GATC	8 cut(s)	50, 462, 481, 670, 745, 830, 910, 1249
Sau96I	GGNCC	4 cut(s)	199, 236, 288, 601
ScaI	AGTACT	1 cut(s)	842
SchI	GAGTC	1 cut(s)	634
ScrFI	CCNGG	5 cut(s)	23, 100, 210, 286, 729
SduI	GDGCHC	4 cut(s)	84, 195, 351, 968
SfaNI	GCATC	4 cut(s)	480, 874, 947, 1270
Sfr274I	CTCGAG	2 cut(s)	203, 825
SinI	GGWCC	2 cut(s)	199, 601
SlaI	CTCGAG	2 cut(s)	203, 825
SmiMI	CAYNNNNRTG	1 cut(s)	1160
SmlI	CTYRAG	3 cut(s)	203, 644, 825
SmoI	CTYRAG	3 cut(s)	203, 644, 825
Sse9I	AATT	4 cut(s)	298, 473, 734, 1108
SseBI	AGGCCT	1 cut(s)	1178
SspMI	CTAG	1 cut(s)	758
StuI	AGGCCT	1 cut(s)	1178
StyD4I	CCNGG	5 cut(s)	21, 98, 208, 284, 727
StyI	CCWWGG	1 cut(s)	216
TaaI	ACNGT	4 cut(s)	282, 535, 845, 1272
TaiI	ACGT	3 cut(s)	180, 928, 1311
TasI	AATT	4 cut(s)	298, 473, 734, 1108
TatI	WGTACW	2 cut(s)	105, 840
TauI	GCSGC	3 cut(s)	168, 171, 1075
TfiI	GAWTC	4 cut(s)	10, 341, 676, 1033
Tru1I	TTAA	1 cut(s)	770
Tru9I	TTAA	1 cut(s)	770
TscAI	CASTG	3 cut(s)	124, 538, 1193
TseFI	GTSAC	3 cut(s)	380, 407, 1061
TseI	GCWGC	2 cut(s)	835, 905
Tsp45I	GTSAC	3 cut(s)	380, 407, 1061
TspDTI	ATGAA	6 cut(s)	627, 787, 1007, 1090, 1259, 1308
TspGWI	ACGGA	4 cut(s)	335, 553, 936, 1241
TspRI	CASTG	3 cut(s)	124, 538, 1193
Van91I	CCANNNNNTGG	1 cut(s)	665
VpaK11BI	GGWCC	2 cut(s)	199, 601
XapI	RAATTY	2 cut(s)	473, 1108
XhoI	CTCGAG	2 cut(s)	203, 825
XmiI	GTMKAC	2 cut(s)	978, 1193
XmnI	GAANNNNTTC	2 cut(s)	1032, 1178
XspI	CTAG	1 cut(s)	758
ZrmI	AGTACT	1 cut(s)	842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.