Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g17280 |
| malus_domestica | MD06G1226500.v1.1 MD14G1235900.v1.1 |
| prunus_persica | Prupe.5G232700_v2.0.a1 |
| pyrus_communis | pycom06g20280 |
| rosa_chinensis | RchiOBHm_Chr7g0178491 |
| rosa_laevigata | RLG00000005442 |
| rosa_multiflora | Rmu_sc0010235.1_g000002 |
| rosa_rugosa | Rorug06G0417600 |
| rosa_wichuraiana | Rw7G001270 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 549 |
| AccB1I | GGYRCC | 1 cut(s) | 212 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 60, 665, 920 |
| AccBSI | CCGCTC | 1 cut(s) | 702 |
| AccI | GTMKAC | 1 cut(s) | 1193 |
| AciI | CCGC | 8 cut(s) | 165, 168, 188, 587, 702, 761, 942, 1232 |
| AclWI | GGATC | 5 cut(s) | 45, 58, 766, 838, 905 |
| AcoI | YGGCCR | 4 cut(s) | 100, 169, 244, 922 |
| AcsI | RAATTY | 2 cut(s) | 473, 1108 |
| AcuI | CTGAAG | 1 cut(s) | 619 |
| AfaI | GTAC | 2 cut(s) | 107, 842 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 60, 665, 920, 1076 |
| AgsI | TTSAA | 3 cut(s) | 401, 676, 1292 |
| AjnI | CCWGG | 3 cut(s) | 21, 208, 727 |
| AleI | CACNNNNGTG | 1 cut(s) | 536 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 995, 1027 |
| AluBI | AGCT | 6 cut(s) | 334, 617, 649, 663, 736, 757 |
| AluI | AGCT | 6 cut(s) | 334, 617, 649, 663, 736, 757 |
| Alw21I | GWGCWC | 4 cut(s) | 84, 195, 336, 968 |
| AlwI | GGATC | 5 cut(s) | 45, 58, 766, 838, 905 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 533 |
| Ama87I | CYCGRG | 2 cut(s) | 183, 825 |
| ApeKI | GCWGC | 1 cut(s) | 905 |
| ApoI | RAATTY | 2 cut(s) | 473, 1108 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1178 |
| AspLEI | GCGC | 1 cut(s) | 329 |
| AspS9I | GGNCC | 7 cut(s) | 199, 231, 236, 258, 288, 303, 601 |
| AsuC2I | CCSGG | 5 cut(s) | 112, 184, 185, 230, 479 |
| AsuHPI | GGTGA | 4 cut(s) | 487, 560, 643, 775 |
| AsuNHI | GCTAGC | 1 cut(s) | 757 |
| AvaI | CYCGRG | 2 cut(s) | 183, 825 |
| AvaII | GGWCC | 3 cut(s) | 199, 236, 601 |
| BaeGI | GKGCMC | 1 cut(s) | 183 |
| BalI | TGGCCA | 1 cut(s) | 246 |
| BamHI | GGATCC | 1 cut(s) | 50 |
| BanI | GGYRCC | 1 cut(s) | 212 |
| BanII | GRGCYC | 1 cut(s) | 336 |
| BbsI | GAAGAC | 2 cut(s) | 265, 1083 |
| Bbv12I | GWGCWC | 4 cut(s) | 84, 195, 336, 968 |
| BbvI | GCAGC | 1 cut(s) | 892 |
| BccI | CCATC | 2 cut(s) | 520, 1210 |
| BceAI | ACGGC | 4 cut(s) | 100, 156, 909, 1173 |
| BciT130I | CCWGG | 3 cut(s) | 23, 210, 729 |
| BcnI | CCSGG | 5 cut(s) | 112, 184, 185, 230, 479 |
| BfaI | CTAG | 1 cut(s) | 758 |
| BfoI | RGCGCY | 1 cut(s) | 330 |
| BisI | GCNGC | 3 cut(s) | 166, 169, 906 |
| BlpI | GCTNAGC | 1 cut(s) | 330 |
| BlsI | GCNGC | 3 cut(s) | 167, 170, 907 |
| BmcAI | AGTACT | 1 cut(s) | 842 |
| Bme1390I | CCNGG | 8 cut(s) | 23, 112, 184, 185, 210, 230, 479, 729 |
| Bme18I | GGWCC | 3 cut(s) | 199, 236, 601 |
| BmeT110I | CYCGRG | 2 cut(s) | 183, 825 |
| BmgT120I | GGNCC | 7 cut(s) | 199, 231, 236, 258, 288, 303, 601 |
| BmiI | GGNNCC | 6 cut(s) | 41, 52, 214, 238, 289, 543 |
| BmrFI | CCNGG | 8 cut(s) | 23, 112, 184, 185, 210, 230, 479, 729 |
| BmrI | ACTGGG | 2 cut(s) | 425, 438 |
| BmsI | GCATC | 5 cut(s) | 234, 480, 874, 947, 1270 |
| BmtI | GCTAGC | 1 cut(s) | 761 |
| BmuI | ACTGGG | 2 cut(s) | 425, 438 |
| BpiI | GAAGAC | 2 cut(s) | 265, 1083 |
| BpmI | CTGGAG | 1 cut(s) | 1218 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 330 |
| BpuEI | CTTGAG | 2 cut(s) | 224, 629 |
| BpuMI | CCSGG | 5 cut(s) | 112, 184, 185, 230, 479 |
| BsaAI | YACGTR | 1 cut(s) | 178 |
| BsaBI | GATNNNNATC | 1 cut(s) | 675 |
| BsaJI | CCNNGG | 8 cut(s) | 22, 182, 183, 216, 727, 919, 969, 1284 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 995, 1025, 1129, 1159 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 60, 665, 920, 1076 |
| Bse118I | RCCGGY | 2 cut(s) | 98, 290 |
| Bse1I | ACTGG | 7 cut(s) | 103, 420, 433, 579, 1053, 1193, 1201 |
| Bse3DI | GCAATG | 1 cut(s) | 38 |
| Bse8I | GATNNNNATC | 1 cut(s) | 675 |
| BseBI | CCWGG | 3 cut(s) | 23, 210, 729 |
| BseDI | CCNNGG | 8 cut(s) | 22, 182, 183, 216, 727, 919, 969, 1284 |
| BseGI | GGATG | 7 cut(s) | 18, 225, 595, 889, 938, 1065, 1216 |
| BseJI | GATNNNNATC | 1 cut(s) | 675 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 60, 665, 920, 1076 |
| BseMI | GCAATG | 1 cut(s) | 38 |
| BseMII | CTCAG | 2 cut(s) | 321, 756 |
| BseNI | ACTGG | 7 cut(s) | 103, 420, 433, 579, 1053, 1193, 1201 |
| BseRI | GAGGAG | 1 cut(s) | 1003 |
| BseSI | GKGCMC | 1 cut(s) | 183 |
| BseX3I | CGGCCG | 1 cut(s) | 169 |
| BseXI | GCAGC | 1 cut(s) | 892 |
| BsgI | GTGCAG | 1 cut(s) | 1019 |
| Bsh1285I | CGRYCG | 1 cut(s) | 172 |
| BshNI | GGYRCC | 1 cut(s) | 212 |
| BsiEI | CGRYCG | 1 cut(s) | 172 |
| BsiHKAI | GWGCWC | 4 cut(s) | 84, 195, 336, 968 |
| BsiHKCI | CYCGRG | 2 cut(s) | 183, 825 |
| BsiSI | CCGG | 8 cut(s) | 99, 111, 184, 229, 234, 256, 291, 479 |
| BslFI | GGGAC | 2 cut(s) | 842, 1342 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 60, 665, 920, 1076 |
| BsmFI | GGGAC | 2 cut(s) | 842, 1342 |
| BsmI | GAATGC | 1 cut(s) | 589 |
| BsoBI | CYCGRG | 2 cut(s) | 183, 825 |
| Bsp1286I | GDGCHC | 5 cut(s) | 84, 183, 195, 336, 968 |
| Bsp143I | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 330 |
| Bsp19I | CCATGG | 2 cut(s) | 216, 919 |
| BspACI | CCGC | 8 cut(s) | 165, 168, 188, 587, 702, 761, 942, 1232 |
| BspCNI | CTCAG | 2 cut(s) | 322, 755 |
| BspLI | GGNNCC | 6 cut(s) | 41, 52, 214, 238, 289, 543 |
| BspOI | GCTAGC | 1 cut(s) | 761 |
| BspPI | GGATC | 5 cut(s) | 45, 58, 766, 838, 905 |
| BspT107I | GGYRCC | 1 cut(s) | 212 |
| BsrBI | CCGCTC | 1 cut(s) | 702 |
| BsrDI | GCAATG | 1 cut(s) | 38 |
| BsrFI | RCCGGY | 2 cut(s) | 98, 290 |
| BsrI | ACTGG | 7 cut(s) | 103, 420, 433, 579, 1053, 1193, 1201 |
| BssAI | RCCGGY | 2 cut(s) | 98, 290 |
| BssECI | CCNNGG | 8 cut(s) | 22, 182, 183, 216, 727, 919, 969, 1284 |
| BssMI | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| BssNAI | GTATAC | 1 cut(s) | 1194 |
| BssT1I | CCWWGG | 3 cut(s) | 216, 919, 969 |
| Bst1107I | GTATAC | 1 cut(s) | 1194 |
| Bst2UI | CCWGG | 3 cut(s) | 23, 210, 729 |
| Bst4CI | ACNGT | 4 cut(s) | 380, 535, 1152, 1288 |
| BstBAI | YACGTR | 1 cut(s) | 178 |
| BstC8I | GCNNGC | 4 cut(s) | 163, 292, 389, 759 |
| BstDEI | CTNAG | 3 cut(s) | 330, 742, 851 |
| BstDSI | CCRYGG | 3 cut(s) | 216, 919, 1284 |
| BstF5I | GGATG | 7 cut(s) | 18, 225, 595, 889, 938, 1065, 1216 |
| BstH2I | RGCGCY | 1 cut(s) | 330 |
| BstHHI | GCGC | 1 cut(s) | 329 |
| BstKTI | GATC | 6 cut(s) | 53, 465, 774, 833, 913, 1252 |
| BstMBI | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| BstMCI | CGRYCG | 1 cut(s) | 172 |
| BstNI | CCWGG | 3 cut(s) | 23, 210, 729 |
| BstSCI | CCNGG | 8 cut(s) | 21, 110, 182, 183, 208, 228, 477, 727 |
| BstSLI | GKGCMC | 1 cut(s) | 183 |
| BstV1I | GCAGC | 1 cut(s) | 892 |
| BstV2I | GAAGAC | 2 cut(s) | 265, 1083 |
| BstX2I | RGATCY | 1 cut(s) | 50 |
| BstYI | RGATCY | 1 cut(s) | 50 |
| BstZ17I | GTATAC | 1 cut(s) | 1194 |
| BstZI | CGGCCG | 1 cut(s) | 169 |
| BtgI | CCRYGG | 3 cut(s) | 216, 919, 1284 |
| BtsCI | GGATG | 7 cut(s) | 18, 225, 595, 889, 938, 1065, 1216 |
| BtsIMutI | CAGTG | 4 cut(s) | 413, 531, 1060, 1186 |
| Cac8I | GCNNGC | 4 cut(s) | 163, 292, 389, 759 |
| CaiI | CAGNNNCTG | 1 cut(s) | 533 |
| CfoI | GCGC | 1 cut(s) | 329 |
| Cfr10I | RCCGGY | 2 cut(s) | 98, 290 |
| Cfr13I | GGNCC | 7 cut(s) | 199, 231, 236, 258, 288, 303, 601 |
| Cfr9I | CCCGGG | 1 cut(s) | 183 |
| CseI | GACGC | 1 cut(s) | 844 |
| Csp6I | GTAC | 2 cut(s) | 106, 841 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 860, 895 |
| CviAII | CATG | 8 cut(s) | 196, 217, 799, 895, 920, 946, 1087, 1243 |
| CviQI | GTAC | 2 cut(s) | 106, 841 |
| DdeI | CTNAG | 3 cut(s) | 330, 742, 851 |
| DpnI | GATC | 6 cut(s) | 52, 464, 773, 832, 912, 1251 |
| DpnII | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| EaeI | YGGCCR | 4 cut(s) | 100, 169, 244, 922 |
| EagI | CGGCCG | 1 cut(s) | 169 |
| EciI | GGCGGA | 2 cut(s) | 931, 1247 |
| Ecl136II | GAGCTC | 1 cut(s) | 334 |
| EclXI | CGGCCG | 1 cut(s) | 169 |
| Eco130I | CCWWGG | 3 cut(s) | 216, 919, 969 |
| Eco147I | AGGCCT | 1 cut(s) | 1178 |
| Eco24I | GRGCYC | 1 cut(s) | 336 |
| Eco32I | GATATC | 1 cut(s) | 628 |
| Eco47I | GGWCC | 3 cut(s) | 199, 236, 601 |
| Eco52I | CGGCCG | 1 cut(s) | 169 |
| Eco53kI | GAGCTC | 1 cut(s) | 334 |
| Eco57I | CTGAAG | 1 cut(s) | 619 |
| Eco88I | CYCGRG | 2 cut(s) | 183, 825 |
| EcoICRI | GAGCTC | 1 cut(s) | 334 |
| EcoRI | GAATTC | 2 cut(s) | 473, 1108 |
| EcoRII | CCWGG | 3 cut(s) | 21, 208, 727 |
| EcoRV | GATATC | 1 cut(s) | 628 |
| EcoT14I | CCWWGG | 3 cut(s) | 216, 919, 969 |
| EcoT38I | GRGCYC | 1 cut(s) | 336 |
| ErhI | CCWWGG | 3 cut(s) | 216, 919, 969 |
| FaeI | CATG | 8 cut(s) | 199, 220, 802, 898, 923, 949, 1090, 1246 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1166, 1198 |
| FaqI | GGGAC | 2 cut(s) | 842, 1342 |
| FatI | CATG | 8 cut(s) | 195, 216, 798, 894, 919, 945, 1086, 1242 |
| FblI | GTMKAC | 1 cut(s) | 1193 |
| Fnu4HI | GCNGC | 3 cut(s) | 166, 169, 906 |
| FokI | GGATG | 7 cut(s) | 5, 212, 602, 896, 925, 1052, 1223 |
| FriOI | GRGCYC | 1 cut(s) | 336 |
| Fsp4HI | GCNGC | 3 cut(s) | 166, 169, 906 |
| FspBI | CTAG | 1 cut(s) | 758 |
| GlaI | GCGC | 1 cut(s) | 328 |
| GluI | GCNGC | 3 cut(s) | 166, 169, 906 |
| GsuI | CTGGAG | 1 cut(s) | 1218 |
| HaeII | RGCGCY | 1 cut(s) | 330 |
| HapII | CCGG | 8 cut(s) | 99, 111, 184, 229, 234, 256, 291, 479 |
| HgaI | GACGC | 1 cut(s) | 844 |
| HhaI | GCGC | 1 cut(s) | 329 |
| Hin1II | CATG | 8 cut(s) | 199, 220, 802, 898, 923, 949, 1090, 1246 |
| Hin6I | GCGC | 1 cut(s) | 327 |
| HinP1I | GCGC | 1 cut(s) | 327 |
| HindIII | AAGCTT | 1 cut(s) | 661 |
| HinfI | GANTC | 5 cut(s) | 10, 341, 676, 723, 1033 |
| HpaII | CCGG | 8 cut(s) | 99, 111, 184, 229, 234, 256, 291, 479 |
| HphI | GGTGA | 4 cut(s) | 487, 560, 643, 775 |
| Hpy166II | GTNNAC | 7 cut(s) | 108, 568, 766, 1148, 1155, 1194, 1224 |
| Hpy188I | TCNGA | 8 cut(s) | 467, 529, 684, 745, 752, 819, 953, 1038 |
| Hpy188III | TCNNGA | 7 cut(s) | 435, 637, 690, 825, 1135, 1168, 1337 |
| Hpy8I | GTNNAC | 7 cut(s) | 108, 568, 766, 1148, 1155, 1194, 1224 |
| HpyAV | CCTTC | 5 cut(s) | 317, 508, 1168, 1189, 1196 |
| HpyCH4III | ACNGT | 4 cut(s) | 380, 535, 1152, 1288 |
| HpyCH4IV | ACGT | 1 cut(s) | 177 |
| HpyCH4V | TGCA | 6 cut(s) | 16, 391, 812, 1000, 1186, 1259 |
| HpyF3I | CTNAG | 3 cut(s) | 330, 742, 851 |
| HpySE526I | ACGT | 1 cut(s) | 177 |
| Hsp92II | CATG | 8 cut(s) | 199, 220, 802, 898, 923, 949, 1090, 1246 |
| HspAI | GCGC | 1 cut(s) | 327 |
| KroI | GCCGGC | 1 cut(s) | 290 |
| KroNI | GCCGGC | 1 cut(s) | 292 |
| Kzo9I | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| LmnI | GCTCC | 2 cut(s) | 541, 1339 |
| Lsp1109I | GCAGC | 1 cut(s) | 892 |
| LweI | GCATC | 5 cut(s) | 234, 480, 874, 947, 1270 |
| MaeI | CTAG | 1 cut(s) | 758 |
| MaeII | ACGT | 1 cut(s) | 177 |
| MaeIII | GTNAC | 8 cut(s) | 74, 173, 380, 407, 706, 926, 1061, 1238 |
| MalI | GATC | 6 cut(s) | 52, 464, 773, 832, 912, 1251 |
| MbiI | CCGCTC | 1 cut(s) | 702 |
| MboI | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| MboII | GAAGA | 7 cut(s) | 265, 482, 787, 1083, 1304, 1307, 1322 |
| MfeI | CAATTG | 1 cut(s) | 298 |
| MflI | RGATCY | 1 cut(s) | 50 |
| MhlI | GDGCHC | 5 cut(s) | 84, 183, 195, 336, 968 |
| MlsI | TGGCCA | 1 cut(s) | 246 |
| MluCI | AATT | 3 cut(s) | 298, 473, 1108 |
| MluNI | TGGCCA | 1 cut(s) | 246 |
| Mox20I | TGGCCA | 1 cut(s) | 246 |
| MroNI | GCCGGC | 1 cut(s) | 290 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1178 |
| MscI | TGGCCA | 1 cut(s) | 246 |
| MslI | CAYNNNNRTG | 2 cut(s) | 536, 1160 |
| Msp20I | TGGCCA | 1 cut(s) | 246 |
| MspA1I | CMGCKG | 2 cut(s) | 165, 736 |
| MspI | CCGG | 8 cut(s) | 99, 111, 184, 229, 234, 256, 291, 479 |
| MspR9I | CCNGG | 8 cut(s) | 23, 112, 184, 185, 210, 230, 479, 729 |
| MunI | CAATTG | 1 cut(s) | 298 |
| Mva1269I | GAATGC | 1 cut(s) | 589 |
| MvaI | CCWGG | 3 cut(s) | 23, 210, 729 |
| NaeI | GCCGGC | 1 cut(s) | 292 |
| NciI | CCSGG | 5 cut(s) | 112, 184, 185, 230, 479 |
| NcoI | CCATGG | 2 cut(s) | 216, 919 |
| NdeII | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| NgoMIV | GCCGGC | 1 cut(s) | 290 |
| NheI | GCTAGC | 1 cut(s) | 757 |
| NlaIII | CATG | 8 cut(s) | 199, 220, 802, 898, 923, 949, 1090, 1246 |
| NlaIV | GGNNCC | 6 cut(s) | 41, 52, 214, 238, 289, 543 |
| NmuCI | GTSAC | 5 cut(s) | 74, 380, 407, 706, 1061 |
| OliI | CACNNNNGTG | 1 cut(s) | 536 |
| PaeR7I | CTCGAG | 1 cut(s) | 825 |
| PceI | AGGCCT | 1 cut(s) | 1178 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 922 |
| PctI | GAATGC | 1 cut(s) | 589 |
| PdiI | GCCGGC | 1 cut(s) | 292 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1178 |
| PfeI | GAWTC | 5 cut(s) | 10, 341, 676, 723, 1033 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 60, 665, 920 |
| PfoI | TCCNGGA | 1 cut(s) | 477 |
| PkrI | GCNGC | 3 cut(s) | 167, 170, 907 |
| Ppu21I | YACGTR | 1 cut(s) | 178 |
| PsiI | TTATAA | 1 cut(s) | 549 |
| Psp124BI | GAGCTC | 1 cut(s) | 336 |
| Psp6I | CCWGG | 3 cut(s) | 21, 208, 727 |
| PspGI | CCWGG | 3 cut(s) | 21, 208, 727 |
| PspN4I | GGNNCC | 6 cut(s) | 41, 52, 214, 238, 289, 543 |
| PspPI | GGNCC | 7 cut(s) | 199, 231, 236, 258, 288, 303, 601 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1001, 1033 |
| PstNI | CAGNNNCTG | 1 cut(s) | 533 |
| PsuI | RGATCY | 1 cut(s) | 50 |
| PvuII | CAGCTG | 1 cut(s) | 736 |
| RsaI | GTAC | 2 cut(s) | 107, 842 |
| RsaNI | GTAC | 2 cut(s) | 106, 841 |
| RseI | CAYNNNNRTG | 2 cut(s) | 536, 1160 |
| SacI | GAGCTC | 1 cut(s) | 336 |
| SatI | GCNGC | 3 cut(s) | 166, 169, 906 |
| Sau3AI | GATC | 6 cut(s) | 50, 462, 771, 830, 910, 1249 |
| Sau96I | GGNCC | 7 cut(s) | 199, 231, 236, 258, 288, 303, 601 |
| ScaI | AGTACT | 1 cut(s) | 842 |
| ScrFI | CCNGG | 8 cut(s) | 23, 112, 184, 185, 210, 230, 479, 729 |
| SduI | GDGCHC | 5 cut(s) | 84, 183, 195, 336, 968 |
| SfaNI | GCATC | 5 cut(s) | 234, 480, 874, 947, 1270 |
| Sfr274I | CTCGAG | 1 cut(s) | 825 |
| SinI | GGWCC | 3 cut(s) | 199, 236, 601 |
| SlaI | CTCGAG | 1 cut(s) | 825 |
| SmaI | CCCGGG | 1 cut(s) | 185 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 536, 1160 |
| SmlI | CTYRAG | 3 cut(s) | 203, 644, 825 |
| SmoI | CTYRAG | 3 cut(s) | 203, 644, 825 |
| Sse9I | AATT | 3 cut(s) | 298, 473, 1108 |
| SseBI | AGGCCT | 1 cut(s) | 1178 |
| SsiI | CCGC | 8 cut(s) | 165, 168, 188, 587, 702, 761, 942, 1232 |
| SspMI | CTAG | 1 cut(s) | 758 |
| SstI | GAGCTC | 1 cut(s) | 336 |
| StuI | AGGCCT | 1 cut(s) | 1178 |
| StyD4I | CCNGG | 8 cut(s) | 21, 110, 182, 183, 208, 228, 477, 727 |
| StyI | CCWWGG | 3 cut(s) | 216, 919, 969 |
| TaaI | ACNGT | 4 cut(s) | 380, 535, 1152, 1288 |
| TaiI | ACGT | 1 cut(s) | 180 |
| TaqI | TCGA | 5 cut(s) | 784, 826, 978, 1094, 1302 |
| TasI | AATT | 3 cut(s) | 298, 473, 1108 |
| TatI | WGTACW | 2 cut(s) | 105, 840 |
| TauI | GCSGC | 2 cut(s) | 168, 171 |
| TfiI | GAWTC | 5 cut(s) | 10, 341, 676, 723, 1033 |
| TscAI | CASTG | 4 cut(s) | 420, 538, 1060, 1193 |
| TseFI | GTSAC | 5 cut(s) | 74, 380, 407, 706, 1061 |
| TseI | GCWGC | 1 cut(s) | 905 |
| Tsp45I | GTSAC | 5 cut(s) | 74, 380, 407, 706, 1061 |
| TspDTI | ATGAA | 7 cut(s) | 627, 787, 996, 1007, 1090, 1259, 1323 |
| TspGWI | ACGGA | 1 cut(s) | 1241 |
| TspMI | CCCGGG | 1 cut(s) | 183 |
| TspRI | CASTG | 4 cut(s) | 420, 538, 1060, 1193 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 60, 665, 920 |
| VpaK11BI | GGWCC | 3 cut(s) | 199, 236, 601 |
| XapI | RAATTY | 2 cut(s) | 473, 1108 |
| XhoI | CTCGAG | 1 cut(s) | 825 |
| XmaI | CCCGGG | 1 cut(s) | 183 |
| XmiI | GTMKAC | 1 cut(s) | 1193 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1178 |
| XspI | CTAG | 1 cut(s) | 758 |
| ZrmI | AGTACT | 1 cut(s) | 842 |