MD07G1073900.v1.1

protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0019577
Physical Location & Seq
Reverse (-)
7017507 .. 7021706
4200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1073900.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGAGACTATTCGAAAGCTCCAAACTCAGCCTCTTCAAGTTCAAAGCTTCTCTATTAAGCTCACAAGTGACCCCTTTTGGCCCCTTCAATTCCTTTGTACAAAAAAGATGGCTAAAGCCTGTAATTTCAGCCCGAACACGATTAGAAGATCGAACCAGGGACTCAAAGCTAGACAGACTCACAACCCACCTCAAGAAGCTCGATATCATCCTAAAACTCCACGACCTCATGTCCAGTCGGAAGCGTGGACCCTTTGTGTCCTTGCAACTAATGTCTCGATGGAGGAACCTTGTGGGGCTTAATGTAGGCATTGGTGCCTTTGTTCACAAATACCCACATATTTTTGAGGTGTTTACGCATCCGGTGCGGCGCAGTTTGTGTTGCAGGATCAGTAAGAAAATGAGGGGTTTAATAGAGGAAGAGGCTATGGCTATGAAACAATGGGAATTCGAAGTTGTTCATCGTGTAAAGAGGTTGTTGATGATGTCTGTGAATGGCACTCTTCATGTCCATGCTTTGAGGCTGGTCAGGAAAGAATTTGGATTGCCCGAGGATTTCAGAGAATCCATTCTTGGTAAATATTCAAAGGATTTTAGGTTGATTGATTTAGAGGTTGTAGAACTGATTAGTAGAGATGAGAATTTGGATGTTGCTGAAGTTGAGAAATGGCGAGAAAAGGAATTTAGAGAGAAGTGGTTGAGTGAGTTCGAGACAAAGTATGCATTCCCTATAAATTTCCCAACAGGGTATGAAATCCAGGCAGGATTTAGAGATAAGTTGAAGAATTGGCAAAGGCTTCCTTATGTGAAGCCTTATGACAGGAAAGAGATTGCCAGCATTCGTACTTGTGGAGGGATAGAGAGGTATGAGAAACGGGCGGTTGGTATTCTTCACGAGTTCTTGAGTTTGTCAGTAGAGAAGATGGTTGAGGTTGAAAGATTAGCTCATTTTCGGAAAGATTTTGCTATGGTTGTTAATGTGCGGGAGCTTATCTTGAAGCACCCTGGAATTTTTTACATATCAACCAGACGTAATACTCAAACCGTTTTTCTTAGAGAGGCATATAGTAAAGGGTGCTTAATTGAGTCTAATCCGATATATGCTGTTAGGAGGAAAACTTTAGACCTTGTTTTCCTAGGGTGTCGAAATACTAGAGGAATGCCAGCTCCAGAGGAAATTAAGGCGTTCAAAGTAGATGGGGATGTCAAAATGGATGGTGACTGGGTTATTCCAATGTTAGAGAACTTGGACAATGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

421

Amino Acids

49.35

Weight (kDa)

9.72

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 53 - 379 1.9e-109 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45790 AT5G45790
malus_domestica MD02G1242700.v1.1 MD07G1073900.v1.1
prunus_persica Prupe.2G091800_v2.0.a1
pyrus_communis pycom02g20950 pycom07g05800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 314
AciI CCGC 3 cut(s) 367, 878, 982
AclWI GGATC 1 cut(s) 395
AcsI RAATTY 6 cut(s) 446, 536, 640, 680, 733, 1008
AcuI CTGAAG 1 cut(s) 675
AfaI GTAC 2 cut(s) 99, 844
AgsI TTSAA 8 cut(s) 37, 43, 88, 585, 781, 935, 997, 1189
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 3 cut(s) 155, 756, 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 8 cut(s) 18, 47, 60, 169, 199, 944, 988, 1166
AluI AGCT 8 cut(s) 18, 47, 60, 169, 199, 944, 988, 1166
Alw26I GTCTC 2 cut(s) 279, 704
AlwI GGATC 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 1 cut(s) 79
ApoI RAATTY 6 cut(s) 446, 536, 640, 680, 733, 1008
Asp700I GAANNNNTTC 2 cut(s) 456, 567
AspA2I CCTAGG 1 cut(s) 1135
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 80, 248
AsuHPI GGTGA 1 cut(s) 1229
AsuII TTCGAA 2 cut(s) 12, 450
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 1135
BanI GGYRCC 1 cut(s) 314
BauI CACGAG 1 cut(s) 893
BccI CCATC 5 cut(s) 102, 273, 916, 1190, 1208
BciT130I CCWGG 3 cut(s) 157, 758, 1005
BcoDI GTCTC 2 cut(s) 279, 704
BfaI CTAG 3 cut(s) 170, 1136, 1152
BisI GCNGC 1 cut(s) 368
BlnI CCTAGG 1 cut(s) 1135
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 3 cut(s) 157, 758, 1005
Bme18I GGWCC 1 cut(s) 248
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 80, 248
BmiI GGNNCC 4 cut(s) 82, 250, 287, 316
BmrFI CCNGG 3 cut(s) 157, 758, 1005
BmrI ACTGGG 1 cut(s) 1231
BmsI GCATC 1 cut(s) 367
BmuI ACTGGG 1 cut(s) 1231
BpmI CTGGAG 1 cut(s) 1152
Bpu14I TTCGAA 2 cut(s) 12, 450
BpuEI CTTGAG 2 cut(s) 176, 922
BsaJI CCNNGG 4 cut(s) 156, 549, 1003, 1135
BsaWI WCCGGW 1 cut(s) 361
Bse1I ACTGG 2 cut(s) 234, 1226
BseBI CCWGG 3 cut(s) 157, 758, 1005
BseDI CCNNGG 4 cut(s) 156, 549, 1003, 1135
BseGI GGATG 5 cut(s) 207, 358, 652, 1207, 1219
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 2 cut(s) 234, 1226
BshFI GGCC 1 cut(s) 81
BshNI GGYRCC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 1 cut(s) 173
BsmAI GTCTC 2 cut(s) 279, 704
BsmFI GGGAC 1 cut(s) 173
BsmI GAATGC 3 cut(s) 722, 837, 1164
BsnI GGCC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 548
Bsp119I TTCGAA 2 cut(s) 12, 450
Bsp1407I TGTACA 1 cut(s) 97
Bsp143I GATC 2 cut(s) 148, 387
BspACI CCGC 3 cut(s) 367, 878, 982
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 4 cut(s) 82, 250, 287, 316
BspPI GGATC 1 cut(s) 395
BspT104I TTCGAA 2 cut(s) 12, 450
BspT107I GGYRCC 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 97
BsrI ACTGG 2 cut(s) 234, 1226
BssECI CCNNGG 4 cut(s) 156, 549, 1003, 1135
BssMI GATC 2 cut(s) 148, 387
BssSI CACGAG 1 cut(s) 893
BssT1I CCWWGG 1 cut(s) 1135
Bst2BI CACGAG 1 cut(s) 893
Bst2UI CCWGG 3 cut(s) 157, 758, 1005
Bst4CI ACNGT 1 cut(s) 1045
Bst6I CTCTTC 3 cut(s) 38, 414, 507
BstAPI GCANNNNNTGC 1 cut(s) 364
BstAUI TGTACA 1 cut(s) 97
BstBI TTCGAA 2 cut(s) 12, 450
BstC8I GCNNGC 2 cut(s) 835, 1164
BstDEI CTNAG 2 cut(s) 26, 1052
BstF5I GGATG 5 cut(s) 207, 358, 652, 1207, 1219
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 2 cut(s) 151, 390
BstMAI GTCTC 2 cut(s) 279, 704
BstMBI GATC 2 cut(s) 148, 387
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 3 cut(s) 157, 758, 1005
BstSCI CCNGG 3 cut(s) 155, 756, 1003
BsuRI GGCC 1 cut(s) 81
BtsCI GGATG 5 cut(s) 207, 358, 652, 1207, 1219
Cac8I GCNNGC 2 cut(s) 835, 1164
CfoI GCGC 1 cut(s) 372
Cfr13I GGNCC 2 cut(s) 80, 248
Csp6I GTAC 2 cut(s) 98, 843
CviAII CATG 3 cut(s) 229, 506, 512
CviQI GTAC 2 cut(s) 98, 843
DdeI CTNAG 2 cut(s) 26, 1052
DpnI GATC 2 cut(s) 150, 389
DpnII GATC 2 cut(s) 148, 387
DriI GACNNNNNGTC 1 cut(s) 229
Eam1104I CTCTTC 3 cut(s) 38, 414, 507
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 3 cut(s) 38, 414, 507
Eco130I CCWWGG 1 cut(s) 1135
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 155, 756, 1003
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 1135
EcoT22I ATGCAT 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 1135
FaeI CATG 3 cut(s) 232, 509, 515
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FaqI GGGAC 1 cut(s) 173
FatI CATG 3 cut(s) 228, 505, 511
FauI CCCGC 1 cut(s) 975
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 5 cut(s) 194, 345, 659, 1214, 1226
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 3 cut(s) 170, 1136, 1152
GlaI GCGC 1 cut(s) 371
GluI GCNGC 1 cut(s) 368
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 362
HhaI GCGC 1 cut(s) 372
Hin1II CATG 3 cut(s) 232, 509, 515
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 45
HinfI GANTC 4 cut(s) 161, 177, 563, 1085
HpaII CCGG 1 cut(s) 362
HphI GGTGA 1 cut(s) 1229
Hpy166II GTNNAC 3 cut(s) 248, 325, 354
Hpy188I TCNGA 4 cut(s) 240, 560, 954, 1095
Hpy188III TCNNGA 8 cut(s) 193, 276, 529, 709, 893, 901, 994, 1169
Hpy8I GTNNAC 3 cut(s) 248, 325, 354
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 1 cut(s) 1045
HpyCH4IV ACGT 1 cut(s) 1030
HpyCH4V TGCA 3 cut(s) 265, 384, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 2 cut(s) 26, 1052
HpySE526I ACGT 1 cut(s) 1030
Hsp92II CATG 3 cut(s) 232, 509, 515
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 2 cut(s) 148, 387
LmnI GCTCC 3 cut(s) 23, 985, 1171
LweI GCATC 1 cut(s) 367
MaeI CTAG 3 cut(s) 170, 1136, 1152
MaeII ACGT 1 cut(s) 1030
MaeIII GTNAC 2 cut(s) 67, 1217
MalI GATC 2 cut(s) 150, 389
MboI GATC 2 cut(s) 148, 387
MboII GAAGA 7 cut(s) 25, 158, 431, 494, 793, 881, 931
MlyI GAGTC 3 cut(s) 155, 171, 1094
MmeI TCCRAC 1 cut(s) 218
Mph1103I ATGCAT 1 cut(s) 724
MroXI GAANNNNTTC 2 cut(s) 456, 567
MseI TTAA 6 cut(s) 56, 300, 410, 975, 1079, 1179
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 3 cut(s) 157, 758, 1005
Mva1269I GAATGC 3 cut(s) 722, 837, 1164
MvaI CCWGG 3 cut(s) 157, 758, 1005
MwoI GCNNNNNNNGC 1 cut(s) 364
NdeII GATC 2 cut(s) 148, 387
NlaIII CATG 3 cut(s) 232, 509, 515
NlaIV GGNNCC 4 cut(s) 82, 250, 287, 316
NmuCI GTSAC 2 cut(s) 67, 1217
NsiI ATGCAT 1 cut(s) 724
NspV TTCGAA 2 cut(s) 12, 450
PctI GAATGC 3 cut(s) 722, 837, 1164
PdmI GAANNNNTTC 2 cut(s) 456, 567
PfeI GAWTC 1 cut(s) 563
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 3 cut(s) 155, 171, 1093
PpsI GAGTC 3 cut(s) 155, 171, 1093
Psp6I CCWGG 3 cut(s) 155, 756, 1003
PspGI CCWGG 3 cut(s) 155, 756, 1003
PspN4I GGNNCC 4 cut(s) 82, 250, 287, 316
PspPI GGNCC 2 cut(s) 80, 248
RsaI GTAC 2 cut(s) 99, 844
RsaNI GTAC 2 cut(s) 98, 843
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 6 cut(s) 56, 300, 410, 975, 1079, 1179
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 2 cut(s) 148, 387
Sau96I GGNCC 2 cut(s) 80, 248
SchI GAGTC 3 cut(s) 155, 171, 1094
ScrFI CCNGG 3 cut(s) 157, 758, 1005
SfaNI GCATC 1 cut(s) 367
SfuI TTCGAA 2 cut(s) 12, 450
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 2 cut(s) 191, 901
SmoI CTYRAG 2 cut(s) 191, 901
SsiI CCGC 3 cut(s) 367, 878, 982
SspI AATATT 1 cut(s) 581
SspMI CTAG 3 cut(s) 170, 1136, 1152
StyD4I CCNGG 3 cut(s) 155, 756, 1003
StyI CCWWGG 1 cut(s) 1135
TaaI ACNGT 1 cut(s) 1045
TaiI ACGT 1 cut(s) 1033
TaqI TCGA 7 cut(s) 12, 151, 201, 277, 450, 708, 1144
TatI WGTACW 1 cut(s) 97
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 6 cut(s) 56, 300, 410, 975, 1079, 1179
Tru9I TTAA 6 cut(s) 56, 300, 410, 975, 1079, 1179
TseFI GTSAC 2 cut(s) 67, 1217
Tsp45I GTSAC 2 cut(s) 67, 1217
TspDTI ATGAA 4 cut(s) 449, 449, 494, 765
VpaK11BI GGWCC 1 cut(s) 248
XapI RAATTY 6 cut(s) 446, 536, 640, 680, 733, 1008
XmaJI CCTAGG 1 cut(s) 1135
XmnI GAANNNNTTC 2 cut(s) 456, 567
XspI CTAG 3 cut(s) 170, 1136, 1152
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.